Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0000463

Physical Location & Seq

Genomic Coordinates Forward (+)

33355467 .. 33365634

Gene Structure 10168 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1200100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3105 bp

ATGACATTGCTCGGATACTCTAATGCCACTGTTGTTCAATACATTATCGGAATAACTAAACAAGCAAAATCACCGGCTGGTGTTGTAGGCAAGCTTGTAGAATATGGGTTTTCTTCATCAGCTGAAACAAGTGCATTTGCTGAAGACATTTTTTCTAGAGTGCCTCGTAAAGAATCTGGTTTGAACCAATATCAGAAACAAGAGAGGGAAGCTGCAATGTTGGTCAGGAAGCAGCAGACATACGCTCTTTTGGATGCAGATGATGATGACGACGATGATGGAGGTAGAGCATCTGTCCCTGTTGTTTCAGAGTCAAGGAAACCAGATTCCCATAAGAAGCGGTTTAGGAAGAAGGGTTTGAGTCAAGAAGATGAAGAGGATGAGGGGATTGCACAAGAGGAAGAAAAGAGAAGGGTAAAAAGACGAACTTCGCCAGATGAAGATGATGGTTCAGAGTCAGAAGAAGAAAGATTGCGTGATCAAAGAGAGAGGGAGCAATTGGAGCGGAATATAAGGGAGCGGGACACAACAGCAACACGAAAGTTAACAGAGAGAAAGTTGTCAAAAAAGGAAGAAGAGGAGGCTATTCGGAGAACAAATGCTTTTGAGAAAGATGACATTGAAGATTTAAGAAAAGTTTCAAGACAAGAATATCTAAAGAAAAGGGAGCAAAAGAAGTTGGAGGAAATCAGAGACGATTTAGAGGATGAGCAATACTTATTTGATGGCGTGAAACTCACTGAAGCAGAATATCGCGAATTAAGTTACAAGAAACAAATATACGAAATTGTGAGGAAGAGGTCAGATGAGCCTGAGGATGCCAATGAGTACAGGATGCCGGATGCCTATGATGACGAGAATGGTGTTAATCAGGAGAAGAGATTTTCTGTGGCTGTGCAACGCTACAGGGACCAGAGTGCTGGGGATAAAATGAACCCATTTGCAGAACAGGAAGCTTGGGAGGATCACCAAATTGGAAAGGCAACGCTGAAATTTGGTTCAAAAAATAAAAAGCAAATGTCAGATGACTATCAATTTGTATTTGAGGACCAGATTGAGTTTATCAAGGCATCGGTTATGGATGGTGATAAGTTTGATGATGATGGGGAACCCTCCGAGTTAGTTGAGTCGAGGGCGAAATCAGCCTTAGAGAAGCTTCAGGAAGATAGAAAAACATTACCCATCTACACATACCGTGAATCATTGCTCGATGCTATTGAAAAACATCAGGTCCTTGTTATTGTTGGTGAAACAGGTTCTGGAAAAACTACACAGATACCCCAATATCTTCACGAGGCCGGATACACAAAGCGTGGAAAGATTGGATGCACACAACCACGGAGAGTTGCTGCTATGAGTGTTGCTGCAAGGGTTTCTCAAGAAATGGGGGTCAAACTTGGGCATGAGGTTGGTTATTCCATTCGTTTTGAGGATTGTACATCGGAAAAGACTGTTTTGAAATATATGACTGATGGAATGCTGTTGCGAGAATTCCTTGGTGAACCAGATCTGGCTAGCTACAGTGTGGTGATGGTGGACGAGGCCCATGAGCGAACACTGTCAACTGATATTCTATTTGGATTAGTAAAGGATATTGCTCGGTTTAGACCCGATCTTAAACTGCTTATCTCAAGTGCAACGCTTGATGCTGAAAAGTTCAGTGATTATTTTGATTCTGCTCCAATTTTTAAAATTCCTGGGAGGAGGTATCCTGTTGAAATACATTACACGAAAGCACCAGAAGCTGATTACTTAGATGCAGCAATTGTAACTTCACTCCAAATCCATGTGACGCAACCTCCGGGAGATATATTGGTGTTCCTCACTGGTCAAGAAGAAATCGAGACAGCAGAAGAAATAATGAAGCACAGAACACGGGGTCTAGGGACAAAAATTGCTGAGCTGATTATTTGTCCCATATATGCAAACCTACCAACTGAGCTGCAATCAAAAATTTTTGAACCCACACCTGAAGGGGCAAGGAAGGTTGTCCTAGCCACAAATATTGCAGAAACTTCGCTTACCATTGATGGGATCAAATATGTTATTGACCCTGGTTTTTGCAAGATGAAATCCTATAATCCAAGGACTGGTATGGAGTCGTTGTTAATCACTCCCATCTCAAAGGCATCAGCAATGCAGAGGGCAGGTCGATCTGGTCGAACGGGTCCTGGAAAGTGCTACCGCTTATATACTGCCTACAATTATTACAATGATTTGGACGACAACACAGTACCAGAAGTGCAGAGGACTAATCTCGCAAATGTTGTGCTTTCATTGAAGAGCCTTGGTATTCATGACTTGTTACATTTTGATTTTATGGACCCTCCACCATCTGAAGCATTACTAAAAGCCCTGGAACTTTTATTTGCACTAAGTGCATTAAATAAGGTGGGTGAGTTGACTAAAGTTGGTCGAAGGATGGCAGAGTTTCCACTTGACCCCATGCTATCTAAGATGATAGTTGCTTCTGATAAGTACAAGTGTTCAGATGAGATTATTTCCATCGCTGCCATGCTTTCCATTGGTAATTCAATCTTTTACCGTCCAAAGGACAAGCAAGTCCATGCGGACAATGCACGATTGAATTTTCACATGGGGAACGTCGGAGATCACATTGCATTGCTCAAGGTTTATAATTCATGGAAAGAGACCAATTATTCAACCCAGTGGTGTTACGAGAATTATATACAGGTGAGAAGCATGAAGCGTGCTAGAGATATCAGAGACCAGCTCGAGGGACTCTTGGAGAGGGTGGAGATTGAGCTGGTTTCAAATATGAGTGATAACGAGACTATAAAAAAGGCCATTACATCTGGCTTCTTCCCTCATTCTGCAAAGCTTCAAAAGAATGGATCTTATAGAACAGTCAAACATCCGCAGACTGTCCATATACATCCGAGCTCAGGGCTGTCACAGGTGCTTCCGAGATGGGTTATATACCATGAATTGGTACTTACGACCAAGGAATATATGAGACAGGTGACAGAATTAAAGCCAGAGTGGTTAGTGGAAATAGCTCCGCATTATTACCAGCTGAAGGATGTTGAAGATGCGTTATCAAAGAAAATGCCTCGTGGAGAAGGGCGTGCGCAAGAAGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009790 GO:0009791 GO:0009793 GO:0009892 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0016020 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031047 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1035

Amino Acids

118.14

Weight (kDa)

5.59

Isoelectric Point (pI)

45.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Helicase_C	PF00271	597 - 723	1.2e-11	Helicase conserved C-terminal domain
WHD_HA2	PF04408	785 - 813	1e-09	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	814 - 875	3.2e-11	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	933 - 1010	4.9e-24	Oligonucleotide/oligosaccharide-binding (OB)-fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000463)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32490 AT1G32490 AT2G35340 AT2G35340 AT4G16680 AT4G16680
fragaria_vesca	FvH4_1g06760 FvH4_1g06761 FvH4_1g06770 FvH4_3g40380 FvH4_3g40450 FvH4_5g14240 FvH4_5g14240 FvH4_5g14240 FvH4_5g14240 FvH4_7g01330 FvH4_7g01330
malus_domestica	MD02G1072400.v1.1 MD02G1072500.v1.1 MD02G1088100.v1.1 MD06G1200100.v1.1 MD07G1285300.v1.1 MD08G1042900.v1.1
prunus_persica	Prupe.5G201700_v2.0.a1 Prupe.5G201700_v2.0.a1 Prupe.6G014300_v2.0.a1 Prupe.6G159800_v2.0.a1 Prupe.7G201500_v2.0.a1 Prupe.8G103900_v2.0.a1 Prupe.8G104500_v2.0.a1
pyrus_communis	pycom02g05640 pycom02g05650 pycom14g17310
rosa_chinensis	RchiOBHm_Chr2g0092491 RchiOBHm_Chr2g0092541 RchiOBHm_Chr5g0073091 RchiOBHm_Chr6g0263651 RchiOBHm_Chr6g0263711 RchiOBHm_Chr7g0182681
rosa_laevigata	RLG00000005109 RLG00000014320 RLG00000014322 RLG00000014324 RLG00000014326 RLG00000014327 RLG00000016318 RLG00000016322 RLG00000036362
rosa_multiflora	Rmu_sc0000014.1_g000045 Rmu_sc0002174.1_g000001 Rmu_sc0003570.1_g000001 Rmu_sc0004296.1_g000005 Rmu_sc0004296.1_g000014 Rmu_sc0005592.1_g000025
rosa_roxburghii	Rroxscaffold_176G00431160 Rroxscaffold_176G00431170 Rroxscaffold_2G00148990 Rroxscaffold_2G00149040 Rroxscaffold_3G00271070 Rroxscaffold_6G00409880 Rroxscaffold_7G00204020 Rroxscaffold_7G00204040 Rroxscaffold_7G00204070
rosa_rugosa	Rorug02G0027100 Rorug02G0027200 Rorug02G0027300 Rorug05G0421000 Rorug06G0449100
rosa_samantha	Rh2AG072900 Rh2AG073200 Rh2BG073500 Rh2BG074100 Rh2CG074700 Rh2DG071600 Rh2DG072300 Rh5AG478000 Rh5BG498700 Rh5DG510800 Rh6AG125300 Rh6BG121400 Rh6CG119800 Rh7AG052900 Rh7BG052500 Rh7CG054000 Rh7DG052300
rosa_wichuraiana	Rw0G005280 Rw2G005970 Rw2G006000 Rw5G044390 Rw6G010890 Rw7G004310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2637
AasI	GACNNNNNNGTC	1 cut(s)	2561
Acc16I	TGCGCA	1 cut(s)	3093
Acc36I	ACCTGC	1 cut(s)	2138
AccB7I	CCANNNNNTGG	2 cut(s)	2090, 2950
AccBSI	CCGCTC	2 cut(s)	505, 520
AccII	CGCG	1 cut(s)	756
AciI	CCGC	7 cut(s)	340, 505, 520, 2185, 2570, 2879, 3023
AclWI	GGATC	3 cut(s)	972, 2042, 2863
AcsI	RAATTY	5 cut(s)	992, 1490, 1692, 1953, 2587
AcuI	CTGAAG	6 cut(s)	162, 762, 1142, 1992, 2358, 3059
AdeI	CACNNNGTG	1 cut(s)	2378
AfaI	GTAC	5 cut(s)	830, 1438, 2235, 2480, 2955
AfiI	CCNNNNNNNGG	7 cut(s)	2031, 2090, 2551, 2737, 2906, 2950, 3040
AjnI	CCWGG	4 cut(s)	1696, 2053, 2170, 2355
Alw21I	GWGCWC	1 cut(s)	2906
Alw26I	GTCTC	6 cut(s)	687, 1838, 2645, 2721, 2786, 2971
AlwI	GGATC	3 cut(s)	972, 2042, 2863
AlwNI	CAGNNNCTG	2 cut(s)	1259, 1745
Ama87I	CYCGRG	1 cut(s)	2735
AoxI	GGCC	3 cut(s)	1296, 1542, 2805
ApeKI	GCWGC	7 cut(s)	212, 232, 1349, 1364, 1760, 1942, 2510
ApoI	RAATTY	5 cut(s)	992, 1490, 1692, 1953, 2587
ArsI	GACNNNNNNTTYG	2 cut(s)	1019, 1051
Asp700I	GAANNNNTTC	1 cut(s)	637
AspLEI	GCGC	1 cut(s)	3094
AspS9I	GGNCC	6 cut(s)	910, 1048, 1231, 1543, 2168, 2323
AsuC2I	CCSGG	1 cut(s)	1803
AsuHPI	GGTGA	9 cut(s)	63, 959, 1097, 1259, 1511, 1540, 2408, 2707, 2995
AsuNHI	GCTAGC	1 cut(s)	1514
AvaI	CYCGRG	1 cut(s)	2735
AvaII	GGWCC	5 cut(s)	910, 1048, 1231, 2168, 2323
AxyI	CCTNAGG	1 cut(s)	813
BanII	GRGCYC	1 cut(s)	2906
BauI	CACGAG	2 cut(s)	1292, 3075
BbsI	GAAGAC	1 cut(s)	150
Bbv12I	GWGCWC	1 cut(s)	2906
BbvI	GCAGC	7 cut(s)	199, 244, 1336, 1351, 1772, 1929, 2497
BcgI	CGANNNNNNTGC	2 cut(s)	1998, 2032
BciT130I	CCWGG	4 cut(s)	1698, 2055, 2172, 2357
BciVI	GTATCC	3 cut(s)	8, 1295, 1719
BclI	TGATCA	1 cut(s)	478
BcnI	CCSGG	1 cut(s)	1803
BcoDI	GTCTC	6 cut(s)	687, 1838, 2645, 2721, 2786, 2971
BfaI	CTAG	5 cut(s)	156, 1515, 1883, 1994, 2715
BfmI	CTRYAG	2 cut(s)	904, 1519
BfuAI	ACCTGC	1 cut(s)	2138
BfuI	GTATCC	3 cut(s)	8, 1295, 1719
BglII	AGATCT	1 cut(s)	1507
BisI	GCNGC	7 cut(s)	213, 233, 1350, 1365, 1761, 1943, 2511
BlpI	GCTNAGC	1 cut(s)	1899
BlsI	GCNGC	7 cut(s)	214, 234, 1351, 1366, 1762, 1944, 2512
Bme1390I	CCNGG	5 cut(s)	1698, 1803, 2055, 2172, 2357
Bme18I	GGWCC	5 cut(s)	910, 1048, 1231, 2168, 2323
BmeT110I	CYCGRG	1 cut(s)	2735
BmgT120I	GGNCC	6 cut(s)	910, 1048, 1231, 1543, 2168, 2323
BmiI	GGNNCC	4 cut(s)	911, 1110, 2169, 2325
BmrFI	CCNGG	5 cut(s)	1698, 1803, 2055, 2172, 2357
BmrI	ACTGGG	1 cut(s)	2662
BmtI	GCTAGC	1 cut(s)	1518
BmuI	ACTGGG	1 cut(s)	2662
BpiI	GAAGAC	1 cut(s)	150
BplI	GAGNNNNNCTC	2 cut(s)	2718, 2750
Bpu10I	CCTNAGC	1 cut(s)	2905
Bpu1102I	GCTNAGC	1 cut(s)	1899
BpuEI	CTTGAG	3 cut(s)	1362, 1615, 2612
BpuMI	CCSGG	1 cut(s)	1803
BsaBI	GATNNNNATC	1 cut(s)	1029
BsaI	GGTCTC	2 cut(s)	2645, 2721
BsaJI	CCNNGG	8 cut(s)	1337, 1495, 1697, 2053, 2084, 2287, 2355, 2964
BsaXI	ACNNNNNCTCC	4 cut(s)	1783, 1813, 2090, 2120
Bsc4I	CCNNNNNNNGG	7 cut(s)	2031, 2090, 2551, 2737, 2906, 2950, 3040
Bse118I	RCCGGY	1 cut(s)	73
Bse1I	ACTGG	3 cut(s)	1831, 2095, 2668
Bse21I	CCTNAGG	1 cut(s)	813
Bse3DI	GCAATG	6 cut(s)	5, 222, 1202, 2142, 2616, 2621
Bse8I	GATNNNNATC	1 cut(s)	1029
BseBI	CCWGG	4 cut(s)	1698, 2055, 2172, 2357
BseDI	CCNNGG	8 cut(s)	1337, 1495, 1697, 2053, 2084, 2287, 2355, 2964
BseJI	GATNNNNATC	1 cut(s)	1029
BseLI	CCNNNNNNNGG	7 cut(s)	2031, 2090, 2551, 2737, 2906, 2950, 3040
BseMI	GCAATG	6 cut(s)	5, 222, 1202, 2142, 2616, 2621
BseMII	CTCAG	4 cut(s)	804, 1890, 1929, 2919
BseNI	ACTGG	3 cut(s)	1831, 2095, 2668
BseRI	GAGGAG	2 cut(s)	593, 1717
BseXI	GCAGC	7 cut(s)	199, 244, 1336, 1351, 1772, 1929, 2497
BseYI	CCCAGC	1 cut(s)	920
BsgI	GTGCAG	1 cut(s)	2264
Bsh1236I	CGCG	1 cut(s)	756
BshFI	GGCC	3 cut(s)	1298, 1544, 2807
BsiHKAI	GWGCWC	1 cut(s)	2906
BsiHKCI	CYCGRG	1 cut(s)	2735
BsiSI	CCGG	4 cut(s)	74, 839, 1299, 1802
BslFI	GGGAC	6 cut(s)	281, 536, 923, 1899, 1900, 2754
BslI	CCNNNNNNNGG	7 cut(s)	2031, 2090, 2551, 2737, 2906, 2950, 3040
BsmAI	GTCTC	6 cut(s)	687, 1838, 2645, 2721, 2786, 2971
BsmBI	CGTCTC	1 cut(s)	687
BsmFI	GGGAC	6 cut(s)	281, 536, 923, 1899, 1900, 2754
BsmI	GAATGC	1 cut(s)	1482
BsnI	GGCC	3 cut(s)	1298, 1544, 2807
Bso31I	GGTCTC	2 cut(s)	2645, 2721
BsoBI	CYCGRG	1 cut(s)	2735
Bsp1286I	GDGCHC	1 cut(s)	2906
Bsp1407I	TGTACA	1 cut(s)	1436
Bsp143I	GATC	8 cut(s)	478, 964, 1507, 1612, 2034, 2153, 2611, 2855
Bsp1720I	GCTNAGC	1 cut(s)	1899
Bsp68I	TCGCGA	1 cut(s)	756
BspACI	CCGC	7 cut(s)	340, 505, 520, 2185, 2570, 2879, 3023
BspANI	GGCC	3 cut(s)	1298, 1544, 2807
BspCNI	CTCAG	4 cut(s)	805, 1891, 1930, 2918
BspFNI	CGCG	1 cut(s)	756
BspHI	TCATGA	1 cut(s)	2296
BspLI	GGNNCC	4 cut(s)	911, 1110, 2169, 2325
BspMI	ACCTGC	1 cut(s)	2138
BspOI	GCTAGC	1 cut(s)	1518
BspPI	GGATC	3 cut(s)	972, 2042, 2863
BspQI	GCTCTTC	1 cut(s)	2276
BspTNI	GGTCTC	2 cut(s)	2645, 2721
BsrBI	CCGCTC	2 cut(s)	505, 520
BsrDI	GCAATG	6 cut(s)	5, 222, 1202, 2142, 2616, 2621
BsrFI	RCCGGY	1 cut(s)	73
BsrGI	TGTACA	1 cut(s)	1436
BsrI	ACTGG	3 cut(s)	1831, 2095, 2668
BssAI	RCCGGY	1 cut(s)	73
BssECI	CCNNGG	8 cut(s)	1337, 1495, 1697, 2053, 2084, 2287, 2355, 2964
BssMI	GATC	8 cut(s)	478, 964, 1507, 1612, 2034, 2153, 2611, 2855
BssSI	CACGAG	2 cut(s)	1292, 3075
BssT1I	CCWWGG	4 cut(s)	1495, 2084, 2287, 2964
Bst2BI	CACGAG	2 cut(s)	1292, 3075
Bst2UI	CCWGG	4 cut(s)	1698, 2055, 2172, 2357
Bst4CI	ACNGT	9 cut(s)	31, 1196, 1453, 1523, 1560, 2233, 2546, 2869, 2887
Bst6I	CTCTTC	5 cut(s)	369, 570, 791, 872, 2276
BstAPI	GCANNNNNTGC	1 cut(s)	2378
BstAUI	TGTACA	1 cut(s)	1436
BstC8I	GCNNGC	4 cut(s)	92, 1516, 2712, 3090
BstDEI	CTNAG	8 cut(s)	813, 1147, 1753, 1899, 1938, 2375, 2454, 2905
BstDSI	CCRYGG	1 cut(s)	1337
BstFNI	CGCG	1 cut(s)	756
BstHHI	GCGC	1 cut(s)	3094
BstKTI	GATC	8 cut(s)	481, 967, 1510, 1615, 2037, 2156, 2614, 2858
BstMAI	GTCTC	6 cut(s)	687, 1838, 2645, 2721, 2786, 2971
BstMBI	GATC	8 cut(s)	478, 964, 1507, 1612, 2034, 2153, 2611, 2855
BstMWI	GCNNNNNNNGC	5 cut(s)	502, 1142, 1742, 2378, 2576
BstNI	CCWGG	4 cut(s)	1698, 2055, 2172, 2357
BstSCI	CCNGG	5 cut(s)	1696, 1801, 2053, 2170, 2355
BstSFI	CTRYAG	2 cut(s)	904, 1519
BstUI	CGCG	1 cut(s)	756
BstV1I	GCAGC	7 cut(s)	199, 244, 1336, 1351, 1772, 1929, 2497
BstV2I	GAAGAC	1 cut(s)	150
BstX2I	RGATCY	2 cut(s)	1507, 2855
BstXI	CCANNNNNNTGG	1 cut(s)	920
BstYI	RGATCY	2 cut(s)	1507, 2855
Bsu36I	CCTNAGG	1 cut(s)	813
BsuI	GTATCC	3 cut(s)	8, 1295, 1719
BsuRI	GGCC	3 cut(s)	1298, 1544, 2807
BtgI	CCRYGG	1 cut(s)	1337
BtgZI	GCGATG	1 cut(s)	2491
BtsIMutI	CAGTG	7 cut(s)	27, 738, 1528, 1556, 1666, 1824, 2675
BtuMI	TCGCGA	1 cut(s)	756
BveI	ACCTGC	1 cut(s)	2138
Cac8I	GCNNGC	4 cut(s)	92, 1516, 2712, 3090
CaiI	CAGNNNCTG	2 cut(s)	1259, 1745
CciI	TCATGA	1 cut(s)	2296
CfoI	GCGC	1 cut(s)	3094
Cfr10I	RCCGGY	1 cut(s)	73
Cfr13I	GGNCC	6 cut(s)	910, 1048, 1231, 1543, 2168, 2323
CseI	GACGC	1 cut(s)	1801
Csp6I	GTAC	5 cut(s)	829, 1437, 2234, 2479, 2954
CspCI	CAANNNNNGTGG	2 cut(s)	1987, 2022
CviQI	GTAC	5 cut(s)	829, 1437, 2234, 2479, 2954
DdeI	CTNAG	8 cut(s)	813, 1147, 1753, 1899, 1938, 2375, 2454, 2905
DpnI	GATC	8 cut(s)	480, 966, 1509, 1614, 2036, 2155, 2613, 2857
DpnII	GATC	8 cut(s)	478, 964, 1507, 1612, 2034, 2153, 2611, 2855
DraI	TTTAAA	1 cut(s)	1690
DraIII	CACNNNGTG	1 cut(s)	2378
DrdI	GACNNNNNNGTC	1 cut(s)	2561
DseDI	GACNNNNNNGTC	1 cut(s)	2561
Eam1104I	CTCTTC	5 cut(s)	369, 570, 791, 872, 2276
EarI	CTCTTC	5 cut(s)	369, 570, 791, 872, 2276
Ecl136II	GAGCTC	1 cut(s)	2904
Eco130I	CCWWGG	4 cut(s)	1495, 2084, 2287, 2964
Eco24I	GRGCYC	1 cut(s)	2906
Eco31I	GGTCTC	2 cut(s)	2645, 2721
Eco32I	GATATC	1 cut(s)	2722
Eco47I	GGWCC	5 cut(s)	910, 1048, 1231, 2168, 2323
Eco53kI	GAGCTC	1 cut(s)	2904
Eco57I	CTGAAG	6 cut(s)	162, 762, 1142, 1992, 2358, 3059
Eco81I	CCTNAGG	1 cut(s)	813
Eco88I	CYCGRG	1 cut(s)	2735
EcoICRI	GAGCTC	1 cut(s)	2904
EcoO109I	RGGNCCY	2 cut(s)	1231, 2168
EcoRI	GAATTC	1 cut(s)	1490
EcoRII	CCWGG	4 cut(s)	1696, 2053, 2170, 2355
EcoRV	GATATC	1 cut(s)	2722
EcoT14I	CCWWGG	4 cut(s)	1495, 2084, 2287, 2964
EcoT38I	GRGCYC	1 cut(s)	2906
ErhI	CCWWGG	4 cut(s)	1495, 2084, 2287, 2964
Esp3I	CGTCTC	1 cut(s)	687
FalI	AAGNNNNNCTT	2 cut(s)	412, 444
FaqI	GGGAC	6 cut(s)	281, 536, 923, 1899, 1900, 2754
FauI	CCCGC	1 cut(s)	513
FbaI	TGATCA	1 cut(s)	478
Fnu4HI	GCNGC	7 cut(s)	213, 233, 1350, 1365, 1761, 1943, 2511
FriOI	GRGCYC	1 cut(s)	2906
Fsp4HI	GCNGC	7 cut(s)	213, 233, 1350, 1365, 1761, 1943, 2511
FspBI	CTAG	5 cut(s)	156, 1515, 1883, 1994, 2715
FspI	TGCGCA	1 cut(s)	3093
GlaI	GCGC	1 cut(s)	3093
GluI	GCNGC	7 cut(s)	213, 233, 1350, 1365, 1761, 1943, 2511
GsaI	CCCAGC	1 cut(s)	924
HaeIII	GGCC	3 cut(s)	1298, 1544, 2807
HapII	CCGG	4 cut(s)	74, 839, 1299, 1802
HgaI	GACGC	1 cut(s)	1801
HhaI	GCGC	1 cut(s)	3094
Hin6I	GCGC	1 cut(s)	3092
HinP1I	GCGC	1 cut(s)	3092
HincII	GTYRAC	3 cut(s)	546, 1563, 2403
HindII	GTYRAC	3 cut(s)	546, 1563, 2403
HindIII	AAGCTT	4 cut(s)	92, 954, 1154, 2840
HpaI	GTTAAC	1 cut(s)	546
HpaII	CCGG	4 cut(s)	74, 839, 1299, 1802
HphI	GGTGA	9 cut(s)	63, 959, 1097, 1259, 1511, 1540, 2408, 2707, 2995
Hpy166II	GTNNAC	5 cut(s)	546, 1502, 1537, 1563, 2403
Hpy8I	GTNNAC	5 cut(s)	546, 1502, 1537, 1563, 2403
Hpy99I	CGWCG	2 cut(s)	275, 2609
HpyAV	CCTTC	8 cut(s)	346, 405, 1967, 1978, 2412, 3034, 3077, 3092
HpyCH4III	ACNGT	9 cut(s)	31, 1196, 1453, 1523, 1560, 2233, 2546, 2869, 2887
HpyCH4IV	ACGT	1 cut(s)	2604
HpyF10VI	GCNNNNNNNGC	5 cut(s)	502, 1142, 1742, 2378, 2576
HpyF3I	CTNAG	8 cut(s)	813, 1147, 1753, 1899, 1938, 2375, 2454, 2905
HpySE526I	ACGT	1 cut(s)	2604
HspAI	GCGC	1 cut(s)	3092
Ksp22I	TGATCA	1 cut(s)	478
KspAI	GTTAAC	1 cut(s)	546
Kzo9I	GATC	8 cut(s)	478, 964, 1507, 1612, 2034, 2153, 2611, 2855
LguI	GCTCTTC	1 cut(s)	2276
LmnI	GCTCC	6 cut(s)	493, 502, 517, 667, 1684, 3025
Lsp1109I	GCAGC	7 cut(s)	199, 244, 1336, 1351, 1772, 1929, 2497
MaeI	CTAG	5 cut(s)	156, 1515, 1883, 1994, 2715
MaeII	ACGT	1 cut(s)	2604
MaeIII	GTNAC	7 cut(s)	764, 1768, 1789, 2304, 2675, 2913, 2983
MalI	GATC	8 cut(s)	480, 966, 1509, 1614, 2036, 2155, 2613, 2857
MbiI	CCGCTC	2 cut(s)	505, 520
MboI	GATC	8 cut(s)	478, 964, 1507, 1612, 2034, 2153, 2611, 2855
MfeI	CAATTG	2 cut(s)	497, 1764
MflI	RGATCY	2 cut(s)	1507, 2855
MhlI	GDGCHC	1 cut(s)	2906
MlyI	GAGTC	6 cut(s)	320, 370, 464, 1136, 2108, 2736
MmeI	TCCRAC	2 cut(s)	660, 2587
MroXI	GAANNNNTTC	1 cut(s)	637
MseI	TTAA	9 cut(s)	545, 629, 761, 867, 1617, 1689, 2108, 2384, 2993
MspA1I	CMGCKG	2 cut(s)	122, 3037
MspI	CCGG	4 cut(s)	74, 839, 1299, 1802
MspR9I	CCNGG	5 cut(s)	1698, 1803, 2055, 2172, 2357
MunI	CAATTG	2 cut(s)	497, 1764
Mva1269I	GAATGC	1 cut(s)	1482
MvaI	CCWGG	4 cut(s)	1698, 2055, 2172, 2357
MvnI	CGCG	1 cut(s)	756
MwoI	GCNNNNNNNGC	5 cut(s)	502, 1142, 1742, 2378, 2576
NciI	CCSGG	1 cut(s)	1803
NdeII	GATC	8 cut(s)	478, 964, 1507, 1612, 2034, 2153, 2611, 2855
NheI	GCTAGC	1 cut(s)	1514
NlaIV	GGNNCC	4 cut(s)	911, 1110, 2169, 2325
NmuCI	GTSAC	3 cut(s)	1789, 2913, 2983
NruI	TCGCGA	1 cut(s)	756
NsbI	TGCGCA	1 cut(s)	3093
PaeR7I	CTCGAG	1 cut(s)	2735
PagI	TCATGA	1 cut(s)	2296
PciSI	GCTCTTC	1 cut(s)	2276
PcsI	WCGNNNNNNNCGW	1 cut(s)	2158
PctI	GAATGC	1 cut(s)	1482
PdmI	GAANNNNTTC	1 cut(s)	637
PfeI	GAWTC	4 cut(s)	173, 326, 1199, 1673
PflMI	CCANNNNNTGG	2 cut(s)	2090, 2950
PfoI	TCCNGGA	2 cut(s)	1801, 2170
PkrI	GCNGC	7 cut(s)	214, 234, 1351, 1366, 1762, 1944, 2512
PleI	GAGTC	6 cut(s)	319, 369, 463, 1135, 2107, 2736
PpsI	GAGTC	6 cut(s)	319, 369, 463, 1135, 2107, 2736
PpuMI	RGGWCCY	2 cut(s)	1231, 2168
PsiI	TTATAA	1 cut(s)	2637
Psp124BI	GAGCTC	1 cut(s)	2906
Psp5II	RGGWCCY	2 cut(s)	1231, 2168
Psp6I	CCWGG	4 cut(s)	1696, 2053, 2170, 2355
PspFI	CCCAGC	1 cut(s)	920
PspGI	CCWGG	4 cut(s)	1696, 2053, 2170, 2355
PspN4I	GGNNCC	4 cut(s)	911, 1110, 2169, 2325
PspPI	GGNCC	6 cut(s)	910, 1048, 1231, 1543, 2168, 2323
PspPPI	RGGWCCY	2 cut(s)	1231, 2168
PspXI	VCTCGAGB	1 cut(s)	2735
PstNI	CAGNNNCTG	2 cut(s)	1259, 1745
PsuI	RGATCY	2 cut(s)	1507, 2855
PvuII	CAGCTG	2 cut(s)	122, 3037
RruI	TCGCGA	1 cut(s)	756
RsaI	GTAC	5 cut(s)	830, 1438, 2235, 2480, 2955
RsaNI	GTAC	5 cut(s)	829, 1437, 2234, 2479, 2954
SacI	GAGCTC	1 cut(s)	2906
SapI	GCTCTTC	1 cut(s)	2276
SaqAI	TTAA	9 cut(s)	545, 629, 761, 867, 1617, 1689, 2108, 2384, 2993
SatI	GCNGC	7 cut(s)	213, 233, 1350, 1365, 1761, 1943, 2511
Sau3AI	GATC	8 cut(s)	478, 964, 1507, 1612, 2034, 2153, 2611, 2855
Sau96I	GGNCC	6 cut(s)	910, 1048, 1231, 1543, 2168, 2323
SchI	GAGTC	6 cut(s)	320, 370, 464, 1136, 2108, 2736
ScrFI	CCNGG	5 cut(s)	1698, 1803, 2055, 2172, 2357
SduI	GDGCHC	1 cut(s)	2906
SfcI	CTRYAG	2 cut(s)	904, 1519
Sfr274I	CTCGAG	1 cut(s)	2735
SinI	GGWCC	5 cut(s)	910, 1048, 1231, 2168, 2323
SlaI	CTCGAG	1 cut(s)	2735
SmlI	CTYRAG	4 cut(s)	1377, 1630, 2627, 2735
SmoI	CTYRAG	4 cut(s)	1377, 1630, 2627, 2735
SsiI	CCGC	7 cut(s)	340, 505, 520, 2185, 2570, 2879, 3023
SspI	AATATT	1 cut(s)	2005
SspMI	CTAG	5 cut(s)	156, 1515, 1883, 1994, 2715
SstI	GAGCTC	1 cut(s)	2906
StyD4I	CCNGG	5 cut(s)	1696, 1801, 2053, 2170, 2355
StyI	CCWWGG	4 cut(s)	1495, 2084, 2287, 2964
TaaI	ACNGT	9 cut(s)	31, 1196, 1453, 1523, 1560, 2233, 2546, 2869, 2887
TaiI	ACGT	1 cut(s)	2607
TaqI	TCGA	7 cut(s)	1130, 1209, 1842, 2152, 2161, 2416, 2736
TatI	WGTACW	3 cut(s)	828, 1436, 2478
TfiI	GAWTC	4 cut(s)	173, 326, 1199, 1673
Tru1I	TTAA	9 cut(s)	545, 629, 761, 867, 1617, 1689, 2108, 2384, 2993
Tru9I	TTAA	9 cut(s)	545, 629, 761, 867, 1617, 1689, 2108, 2384, 2993
TscAI	CASTG	7 cut(s)	34, 745, 1528, 1563, 1666, 1831, 2675
TseFI	GTSAC	3 cut(s)	1789, 2913, 2983
TseI	GCWGC	7 cut(s)	212, 232, 1349, 1364, 1760, 1942, 2510
Tsp45I	GTSAC	3 cut(s)	1789, 2913, 2983
TspGWI	ACGGA	1 cut(s)	1354
TspRI	CASTG	7 cut(s)	34, 745, 1528, 1563, 1666, 1831, 2675
Van91I	CCANNNNNTGG	2 cut(s)	2090, 2950
VpaK11BI	GGWCC	5 cut(s)	910, 1048, 1231, 2168, 2323
XapI	RAATTY	5 cut(s)	992, 1490, 1692, 1953, 2587
XbaI	TCTAGA	1 cut(s)	155
XhoI	CTCGAG	1 cut(s)	2735
XmnI	GAANNNNTTC	1 cut(s)	637
XspI	CTAG	5 cut(s)	156, 1515, 1883, 1994, 2715

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.