Pre-mRNA-splicing factor ATP-dependent RNA helicase
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| AAA_22 | PF13401 | 103 - 237 | 1.7e-06 | AAA domain |
| WHD_HA2 | PF04408 | 481 - 507 | 2.7e-09 | Helicase associated domain (HA2), winged-helix |
| HA2_C | PF21010 | 508 - 571 | 7.9e-09 | Helicase associated domain (HA2), ratchet-like |
| OB_NTP_bind | PF07717 | 629 - 704 | 4.5e-12 | Oligonucleotide/oligosaccharide-binding (OB)-fold |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1725 |
| AccB1I | GGYRCC | 1 cut(s) | 1761 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2038 |
| AccBSI | CCGCTC | 1 cut(s) | 1828 |
| AccI | GTMKAC | 1 cut(s) | 624 |
| AccIII | TCCGGA | 1 cut(s) | 1636 |
| AciI | CCGC | 4 cut(s) | 215, 1605, 1828, 1964 |
| AclWI | GGATC | 3 cut(s) | 1181, 1400, 1413 |
| AcoI | YGGCCR | 1 cut(s) | 1124 |
| AcsI | RAATTY | 3 cut(s) | 789, 909, 2083 |
| AcuI | CTGAAG | 2 cut(s) | 1440, 1536 |
| AcvI | CACGTG | 1 cut(s) | 829 |
| AcyI | GRCGYC | 1 cut(s) | 5 |
| AdeI | CACNNNGTG | 1 cut(s) | 1460 |
| AfaI | GTAC | 2 cut(s) | 898, 1326 |
| AfeI | AGCGCT | 1 cut(s) | 1425 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 1248, 2038 |
| AflIII | ACRYGT | 2 cut(s) | 828, 1388 |
| AgeI | ACCGGT | 1 cut(s) | 139 |
| AgsI | TTSAA | 5 cut(s) | 518, 676, 908, 1049, 1230 |
| AhlI | ACTAGT | 1 cut(s) | 661 |
| AjnI | CCWGG | 2 cut(s) | 437, 816 |
| Alw21I | GWGCWC | 3 cut(s) | 33, 416, 1571 |
| Alw26I | GTCTC | 5 cut(s) | 21, 130, 954, 1058, 2059 |
| Alw44I | GTGCAC | 1 cut(s) | 412 |
| AlwI | GGATC | 3 cut(s) | 1181, 1400, 1413 |
| Ama87I | CYCGRG | 1 cut(s) | 1159 |
| Aor13HI | TCCGGA | 1 cut(s) | 1636 |
| Aor51HI | AGCGCT | 1 cut(s) | 1425 |
| AoxI | GGCC | 2 cut(s) | 630, 1124 |
| ApaLI | GTGCAC | 1 cut(s) | 412 |
| ApeKI | GCWGC | 4 cut(s) | 446, 449, 887, 977 |
| ApoI | RAATTY | 3 cut(s) | 789, 909, 2083 |
| AsiGI | ACCGGT | 1 cut(s) | 139 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 68, 157 |
| AspLEI | GCGC | 1 cut(s) | 1426 |
| AspS9I | GGNCC | 5 cut(s) | 197, 631, 1788, 1816, 2041 |
| AsuHPI | GGTGA | 6 cut(s) | 102, 154, 806, 1490, 1508, 1622 |
| AsuII | TTCGAA | 1 cut(s) | 787 |
| AvaI | CYCGRG | 1 cut(s) | 1159 |
| AvaII | GGWCC | 4 cut(s) | 197, 1788, 1816, 2041 |
| BaeGI | GKGCMC | 1 cut(s) | 416 |
| BamHI | GGATCC | 1 cut(s) | 1405 |
| BanI | GGYRCC | 1 cut(s) | 1761 |
| BbrPI | CACGTG | 1 cut(s) | 829 |
| Bbv12I | GWGCWC | 3 cut(s) | 33, 416, 1571 |
| BbvI | GCAGC | 4 cut(s) | 433, 436, 899, 989 |
| BccI | CCATC | 4 cut(s) | 554, 613, 697, 1498 |
| BciT130I | CCWGG | 2 cut(s) | 439, 818 |
| BclI | TGATCA | 2 cut(s) | 1300, 1612 |
| BcoDI | GTCTC | 5 cut(s) | 21, 130, 954, 1058, 2059 |
| BcuI | ACTAGT | 1 cut(s) | 661 |
| BfaI | CTAG | 6 cut(s) | 453, 482, 662, 693, 773, 1370 |
| BfmI | CTRYAG | 2 cut(s) | 607, 1987 |
| BfoI | RGCGCY | 1 cut(s) | 1427 |
| BisI | GCNGC | 6 cut(s) | 447, 450, 888, 978, 1829, 1964 |
| BlsI | GCNGC | 6 cut(s) | 448, 451, 889, 979, 1830, 1965 |
| Bme1390I | CCNGG | 2 cut(s) | 439, 818 |
| Bme18I | GGWCC | 4 cut(s) | 197, 1788, 1816, 2041 |
| BmeT110I | CYCGRG | 1 cut(s) | 1159 |
| BmgT120I | GGNCC | 5 cut(s) | 197, 631, 1788, 1816, 2041 |
| BmiI | GGNNCC | 4 cut(s) | 219, 1407, 1763, 1817 |
| BmrFI | CCNGG | 2 cut(s) | 439, 818 |
| BmrI | ACTGGG | 1 cut(s) | 1750 |
| BmsI | GCATC | 7 cut(s) | 583, 874, 1000, 1244, 1375, 1513, 1645 |
| BmuI | ACTGGG | 1 cut(s) | 1750 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1844, 1876 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1247 |
| Bpu14I | TTCGAA | 1 cut(s) | 787 |
| BpuEI | CTTGAG | 3 cut(s) | 447, 1700, 1844 |
| BsaAI | YACGTR | 1 cut(s) | 829 |
| BsaHI | GRCGYC | 1 cut(s) | 5 |
| BsaI | GGTCTC | 2 cut(s) | 130, 1058 |
| BsaWI | WCCGGW | 2 cut(s) | 139, 1636 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 1248, 2038 |
| Bse118I | RCCGGY | 2 cut(s) | 139, 1607 |
| Bse1I | ACTGG | 3 cut(s) | 343, 1756, 1952 |
| Bse3DI | GCAATG | 3 cut(s) | 1303, 1533, 1704 |
| BseAI | TCCGGA | 1 cut(s) | 1636 |
| BseBI | CCWGG | 2 cut(s) | 439, 818 |
| BseGI | GGATG | 4 cut(s) | 889, 1509, 1528, 1633 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 1248, 2038 |
| BseMI | GCAATG | 3 cut(s) | 1303, 1533, 1704 |
| BseMII | CTCAG | 3 cut(s) | 785, 1479, 1764 |
| BseNI | ACTGG | 3 cut(s) | 343, 1756, 1952 |
| BseRI | GAGGAG | 1 cut(s) | 798 |
| BseSI | GKGCMC | 1 cut(s) | 416 |
| BseXI | GCAGC | 4 cut(s) | 433, 436, 899, 989 |
| BseYI | CCCAGC | 1 cut(s) | 1136 |
| BsgI | GTGCAG | 1 cut(s) | 1952 |
| BshFI | GGCC | 2 cut(s) | 632, 1126 |
| BshNI | GGYRCC | 1 cut(s) | 1761 |
| BshTI | ACCGGT | 1 cut(s) | 139 |
| BsiHKAI | GWGCWC | 3 cut(s) | 33, 416, 1571 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1159 |
| BsiSI | CCGG | 4 cut(s) | 42, 140, 1608, 1637 |
| BslFI | GGGAC | 1 cut(s) | 1829 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 1248, 2038 |
| BsmAI | GTCTC | 5 cut(s) | 21, 130, 954, 1058, 2059 |
| BsmBI | CGTCTC | 2 cut(s) | 21, 954 |
| BsmFI | GGGAC | 1 cut(s) | 1829 |
| BsmI | GAATGC | 2 cut(s) | 1677, 2013 |
| BsnI | GGCC | 2 cut(s) | 632, 1126 |
| Bso31I | GGTCTC | 2 cut(s) | 130, 1058 |
| BsoBI | CYCGRG | 1 cut(s) | 1159 |
| Bsp119I | TTCGAA | 1 cut(s) | 787 |
| Bsp1286I | GDGCHC | 3 cut(s) | 33, 416, 1571 |
| Bsp13I | TCCGGA | 1 cut(s) | 1636 |
| Bsp143I | GATC | 8 cut(s) | 955, 1186, 1300, 1405, 1555, 1612, 1699, 1991 |
| BspACI | CCGC | 4 cut(s) | 215, 1605, 1828, 1964 |
| BspANI | GGCC | 2 cut(s) | 632, 1126 |
| BspCNI | CTCAG | 3 cut(s) | 786, 1480, 1763 |
| BspEI | TCCGGA | 1 cut(s) | 1636 |
| BspHI | TCATGA | 2 cut(s) | 913, 1615 |
| BspLI | GGNNCC | 4 cut(s) | 219, 1407, 1763, 1817 |
| BspPI | GGATC | 3 cut(s) | 1181, 1400, 1413 |
| BspT104I | TTCGAA | 1 cut(s) | 787 |
| BspT107I | GGYRCC | 1 cut(s) | 1761 |
| BspTNI | GGTCTC | 2 cut(s) | 130, 1058 |
| BsrBI | CCGCTC | 1 cut(s) | 1828 |
| BsrDI | GCAATG | 3 cut(s) | 1303, 1533, 1704 |
| BsrFI | RCCGGY | 2 cut(s) | 139, 1607 |
| BsrI | ACTGG | 3 cut(s) | 343, 1756, 1952 |
| BssAI | RCCGGY | 2 cut(s) | 139, 1607 |
| BssMI | GATC | 8 cut(s) | 955, 1186, 1300, 1405, 1555, 1612, 1699, 1991 |
| BssNI | GRCGYC | 1 cut(s) | 5 |
| Bst2UI | CCWGG | 2 cut(s) | 439, 818 |
| Bst4CI | ACNGT | 3 cut(s) | 69, 541, 1215 |
| Bst6I | CTCTTC | 1 cut(s) | 1365 |
| BstACI | GRCGYC | 1 cut(s) | 5 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1460 |
| BstBAI | YACGTR | 1 cut(s) | 829 |
| BstBI | TTCGAA | 1 cut(s) | 787 |
| BstC8I | GCNNGC | 2 cut(s) | 1038, 2001 |
| BstDEI | CTNAG | 6 cut(s) | 794, 1247, 1427, 1488, 1536, 1750 |
| BstF5I | GGATG | 4 cut(s) | 889, 1509, 1528, 1633 |
| BstH2I | RGCGCY | 1 cut(s) | 1427 |
| BstHHI | GCGC | 1 cut(s) | 1426 |
| BstKTI | GATC | 8 cut(s) | 958, 1189, 1303, 1408, 1558, 1615, 1702, 1994 |
| BstMAI | GTCTC | 5 cut(s) | 21, 130, 954, 1058, 2059 |
| BstMBI | GATC | 8 cut(s) | 955, 1186, 1300, 1405, 1555, 1612, 1699, 1991 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 443, 851, 1003, 1042, 1460, 1547, 1664, 1922 |
| BstNI | CCWGG | 2 cut(s) | 439, 818 |
| BstNSI | RCATGY | 1 cut(s) | 1036 |
| BstSCI | CCNGG | 2 cut(s) | 437, 816 |
| BstSFI | CTRYAG | 2 cut(s) | 607, 1987 |
| BstSLI | GKGCMC | 1 cut(s) | 416 |
| BstV1I | GCAGC | 4 cut(s) | 433, 436, 899, 989 |
| BstX2I | RGATCY | 1 cut(s) | 1405 |
| BstYI | RGATCY | 1 cut(s) | 1405 |
| BsuRI | GGCC | 2 cut(s) | 632, 1126 |
| BtsCI | GGATG | 4 cut(s) | 889, 1509, 1528, 1633 |
| BtsI | GCAGTG | 1 cut(s) | 1201 |
| BtsIMutI | CAGTG | 3 cut(s) | 951, 1201, 1763 |
| Cac8I | GCNNGC | 2 cut(s) | 1038, 2001 |
| CciI | TCATGA | 2 cut(s) | 913, 1615 |
| CfoI | GCGC | 1 cut(s) | 1426 |
| Cfr10I | RCCGGY | 2 cut(s) | 139, 1607 |
| Cfr13I | GGNCC | 5 cut(s) | 197, 631, 1788, 1816, 2041 |
| CseI | GACGC | 1 cut(s) | 1748 |
| Csp6I | GTAC | 2 cut(s) | 897, 1325 |
| CspAI | ACCGGT | 1 cut(s) | 139 |
| CviQI | GTAC | 2 cut(s) | 897, 1325 |
| DdeI | CTNAG | 6 cut(s) | 794, 1247, 1427, 1488, 1536, 1750 |
| DpnI | GATC | 8 cut(s) | 957, 1188, 1302, 1407, 1557, 1614, 1701, 1993 |
| DpnII | GATC | 8 cut(s) | 955, 1186, 1300, 1405, 1555, 1612, 1699, 1991 |
| DraIII | CACNNNGTG | 1 cut(s) | 1460 |
| EaeI | YGGCCR | 1 cut(s) | 1124 |
| Eam1104I | CTCTTC | 1 cut(s) | 1365 |
| EarI | CTCTTC | 1 cut(s) | 1365 |
| EciI | GGCGGA | 2 cut(s) | 230, 1594 |
| Eco31I | GGTCTC | 2 cut(s) | 130, 1058 |
| Eco47I | GGWCC | 4 cut(s) | 197, 1788, 1816, 2041 |
| Eco47III | AGCGCT | 1 cut(s) | 1425 |
| Eco57I | CTGAAG | 2 cut(s) | 1440, 1536 |
| Eco72I | CACGTG | 1 cut(s) | 829 |
| Eco88I | CYCGRG | 1 cut(s) | 1159 |
| EcoRI | GAATTC | 1 cut(s) | 789 |
| EcoRII | CCWGG | 2 cut(s) | 437, 816 |
| EcoT22I | ATGCAT | 1 cut(s) | 1677 |
| Esp3I | CGTCTC | 2 cut(s) | 21, 954 |
| FaqI | GGGAC | 1 cut(s) | 1829 |
| FauNDI | CATATG | 1 cut(s) | 166 |
| FbaI | TGATCA | 2 cut(s) | 1300, 1612 |
| FblI | GTMKAC | 1 cut(s) | 624 |
| Fnu4HI | GCNGC | 6 cut(s) | 447, 450, 888, 978, 1829, 1964 |
| FokI | GGATG | 4 cut(s) | 896, 1516, 1535, 1620 |
| Fsp4HI | GCNGC | 6 cut(s) | 447, 450, 888, 978, 1829, 1964 |
| FspBI | CTAG | 6 cut(s) | 453, 482, 662, 693, 773, 1370 |
| GlaI | GCGC | 1 cut(s) | 1425 |
| GluI | GCNGC | 6 cut(s) | 447, 450, 888, 978, 1829, 1964 |
| GsaI | CCCAGC | 1 cut(s) | 1140 |
| HaeII | RGCGCY | 1 cut(s) | 1427 |
| HaeIII | GGCC | 2 cut(s) | 632, 1126 |
| HapII | CCGG | 4 cut(s) | 42, 140, 1608, 1637 |
| HgaI | GACGC | 1 cut(s) | 1748 |
| HhaI | GCGC | 1 cut(s) | 1426 |
| Hin1I | GRCGYC | 1 cut(s) | 5 |
| Hin6I | GCGC | 1 cut(s) | 1424 |
| HinP1I | GCGC | 1 cut(s) | 1424 |
| HincII | GTYRAC | 2 cut(s) | 1156, 1218 |
| HindII | GTYRAC | 2 cut(s) | 1156, 1218 |
| HpaI | GTTAAC | 1 cut(s) | 1218 |
| HpaII | CCGG | 4 cut(s) | 42, 140, 1608, 1637 |
| HphI | GGTGA | 6 cut(s) | 102, 154, 806, 1490, 1508, 1622 |
| Hpy166II | GTNNAC | 6 cut(s) | 414, 625, 755, 1156, 1218, 1499 |
| Hpy188I | TCNGA | 8 cut(s) | 52, 58, 532, 795, 1283, 1555, 2016, 2089 |
| Hpy8I | GTNNAC | 6 cut(s) | 414, 625, 755, 1156, 1218, 1499 |
| Hpy99I | CGWCG | 3 cut(s) | 10, 13, 16 |
| HpyAV | CCTTC | 6 cut(s) | 396, 670, 1030, 1081, 1195, 1834 |
| HpyCH4III | ACNGT | 3 cut(s) | 69, 541, 1215 |
| HpyCH4IV | ACGT | 3 cut(s) | 828, 1330, 1390 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 443, 851, 1003, 1042, 1460, 1547, 1664, 1922 |
| HpyF3I | CTNAG | 6 cut(s) | 794, 1247, 1427, 1488, 1536, 1750 |
| HpySE526I | ACGT | 3 cut(s) | 828, 1330, 1390 |
| Hsp92I | GRCGYC | 1 cut(s) | 5 |
| HspAI | GCGC | 1 cut(s) | 1424 |
| Kpn2I | TCCGGA | 1 cut(s) | 1636 |
| Ksp22I | TGATCA | 2 cut(s) | 1300, 1612 |
| KspAI | GTTAAC | 1 cut(s) | 1218 |
| Kzo9I | GATC | 8 cut(s) | 955, 1186, 1300, 1405, 1555, 1612, 1699, 1991 |
| LmnI | GCTCC | 3 cut(s) | 217, 1204, 2113 |
| Lsp1109I | GCAGC | 4 cut(s) | 433, 436, 899, 989 |
| LweI | GCATC | 7 cut(s) | 583, 874, 1000, 1244, 1375, 1513, 1645 |
| MaeI | CTAG | 6 cut(s) | 453, 482, 662, 693, 773, 1370 |
| MaeII | ACGT | 3 cut(s) | 828, 1330, 1390 |
| MaeIII | GTNAC | 1 cut(s) | 2071 |
| MalI | GATC | 8 cut(s) | 957, 1188, 1302, 1407, 1557, 1614, 1701, 1993 |
| MbiI | CCGCTC | 1 cut(s) | 1828 |
| MboI | GATC | 8 cut(s) | 955, 1186, 1300, 1405, 1555, 1612, 1699, 1991 |
| MboII | GAAGA | 4 cut(s) | 194, 530, 1352, 1903 |
| MfeI | CAATTG | 1 cut(s) | 986 |
| MflI | RGATCY | 1 cut(s) | 1405 |
| MhlI | GDGCHC | 3 cut(s) | 33, 416, 1571 |
| MlyI | GAGTC | 4 cut(s) | 47, 85, 1151, 1376 |
| MmeI | TCCRAC | 2 cut(s) | 646, 2019 |
| Mph1103I | ATGCAT | 1 cut(s) | 1677 |
| MroI | TCCGGA | 1 cut(s) | 1636 |
| MroXI | GAANNNNTTC | 2 cut(s) | 68, 157 |
| MseI | TTAA | 5 cut(s) | 705, 1217, 1377, 1466, 2119 |
| MslI | CAYNNNNRTG | 1 cut(s) | 918 |
| MspI | CCGG | 4 cut(s) | 42, 140, 1608, 1637 |
| MspR9I | CCNGG | 2 cut(s) | 439, 818 |
| MunI | CAATTG | 1 cut(s) | 986 |
| Mva1269I | GAATGC | 2 cut(s) | 1677, 2013 |
| MvaI | CCWGG | 2 cut(s) | 439, 818 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 443, 851, 1003, 1042, 1460, 1547, 1664, 1922 |
| NdeI | CATATG | 1 cut(s) | 166 |
| NdeII | GATC | 8 cut(s) | 955, 1186, 1300, 1405, 1555, 1612, 1699, 1991 |
| NlaIV | GGNNCC | 4 cut(s) | 219, 1407, 1763, 1817 |
| NmeAIII | GCCGAG | 1 cut(s) | 1066 |
| NsiI | ATGCAT | 1 cut(s) | 1677 |
| NspI | RCATGY | 1 cut(s) | 1036 |
| NspV | TTCGAA | 1 cut(s) | 787 |
| PagI | TCATGA | 2 cut(s) | 913, 1615 |
| PctI | GAATGC | 2 cut(s) | 1677, 2013 |
| PdmI | GAANNNNTTC | 2 cut(s) | 68, 157 |
| PfeI | GAWTC | 6 cut(s) | 53, 158, 267, 764, 1619, 1972 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2038 |
| PinAI | ACCGGT | 1 cut(s) | 139 |
| PkrI | GCNGC | 6 cut(s) | 448, 451, 889, 979, 1830, 1965 |
| PleI | GAGTC | 4 cut(s) | 46, 84, 1151, 1376 |
| PmaCI | CACGTG | 1 cut(s) | 829 |
| PmlI | CACGTG | 1 cut(s) | 829 |
| PpsI | GAGTC | 4 cut(s) | 46, 84, 1151, 1376 |
| Ppu21I | YACGTR | 1 cut(s) | 829 |
| PsiI | TTATAA | 1 cut(s) | 1725 |
| Psp6I | CCWGG | 2 cut(s) | 437, 816 |
| PspCI | CACGTG | 1 cut(s) | 829 |
| PspFI | CCCAGC | 1 cut(s) | 1136 |
| PspGI | CCWGG | 2 cut(s) | 437, 816 |
| PspN4I | GGNNCC | 4 cut(s) | 219, 1407, 1763, 1817 |
| PspPI | GGNCC | 5 cut(s) | 197, 631, 1788, 1816, 2041 |
| PsuI | RGATCY | 1 cut(s) | 1405 |
| RsaI | GTAC | 2 cut(s) | 898, 1326 |
| RsaNI | GTAC | 2 cut(s) | 897, 1325 |
| RseI | CAYNNNNRTG | 1 cut(s) | 918 |
| SaqAI | TTAA | 5 cut(s) | 705, 1217, 1377, 1466, 2119 |
| SatI | GCNGC | 6 cut(s) | 447, 450, 888, 978, 1829, 1964 |
| Sau3AI | GATC | 8 cut(s) | 955, 1186, 1300, 1405, 1555, 1612, 1699, 1991 |
| Sau96I | GGNCC | 5 cut(s) | 197, 631, 1788, 1816, 2041 |
| SchI | GAGTC | 4 cut(s) | 47, 85, 1151, 1376 |
| ScrFI | CCNGG | 2 cut(s) | 439, 818 |
| SduI | GDGCHC | 3 cut(s) | 33, 416, 1571 |
| SfaNI | GCATC | 7 cut(s) | 583, 874, 1000, 1244, 1375, 1513, 1645 |
| SfcI | CTRYAG | 2 cut(s) | 607, 1987 |
| SfuI | TTCGAA | 1 cut(s) | 787 |
| SgrAI | CRCCGGYG | 1 cut(s) | 1607 |
| SinI | GGWCC | 4 cut(s) | 197, 1788, 1816, 2041 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 918 |
| SmlI | CTYRAG | 3 cut(s) | 462, 1715, 1823 |
| SmoI | CTYRAG | 3 cut(s) | 462, 1715, 1823 |
| SpeI | ACTAGT | 1 cut(s) | 661 |
| SsiI | CCGC | 4 cut(s) | 215, 1605, 1828, 1964 |
| SspMI | CTAG | 6 cut(s) | 453, 482, 662, 693, 773, 1370 |
| StyD4I | CCNGG | 2 cut(s) | 437, 816 |
| TaaI | ACNGT | 3 cut(s) | 69, 541, 1215 |
| TaiI | ACGT | 3 cut(s) | 831, 1333, 1393 |
| TaqI | TCGA | 5 cut(s) | 780, 787, 958, 1385, 2046 |
| TaqII | GACCGA | 1 cut(s) | 185 |
| TatI | WGTACW | 1 cut(s) | 1324 |
| TauI | GCSGC | 2 cut(s) | 1831, 1966 |
| TfiI | GAWTC | 6 cut(s) | 53, 158, 267, 764, 1619, 1972 |
| Tru1I | TTAA | 5 cut(s) | 705, 1217, 1377, 1466, 2119 |
| Tru9I | TTAA | 5 cut(s) | 705, 1217, 1377, 1466, 2119 |
| TscAI | CASTG | 3 cut(s) | 958, 1201, 1763 |
| TseI | GCWGC | 4 cut(s) | 446, 449, 887, 977 |
| TspGWI | ACGGA | 4 cut(s) | 1320, 1516, 1612, 2090 |
| TspRI | CASTG | 3 cut(s) | 958, 1201, 1763 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2038 |
| VneI | GTGCAC | 1 cut(s) | 412 |
| VpaK11BI | GGWCC | 4 cut(s) | 197, 1788, 1816, 2041 |
| XapI | RAATTY | 3 cut(s) | 789, 909, 2083 |
| XbaI | TCTAGA | 1 cut(s) | 692 |
| XceI | RCATGY | 1 cut(s) | 1036 |
| XmiI | GTMKAC | 1 cut(s) | 624 |
| XmnI | GAANNNNTTC | 2 cut(s) | 68, 157 |
| XspI | CTAG | 6 cut(s) | 453, 482, 662, 693, 773, 1370 |
| Zsp2I | ATGCAT | 1 cut(s) | 1677 |