Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7B

Orthogroup ID

OG0000463

Physical Location & Seq

Genomic Coordinates Reverse (-)

3423482 .. 3433020

Gene Structure 9539 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7BG052500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3129 bp

ATGGGGAGCGAGAGCAATTTGAAGACGTGGGTTTCCGATAAGCTGATGACATTGCTCGGTTATTCTCAGCCGGCTGTTGTTCAGTACATTATTGGATTAACTAAACAAGCAAAGTCTCCAGCTGATGTGGTGGAGAAGCTTTTAGATTCGGGGTGGTCCTCCTCGTCTGATACACGTAAATTTGCTGAAGAAATATTCTCCAGAGTGCCCCACAAATCATCCGGACCGAATGTTTATCAGAAACAAGAGAGGGAGGCTGCAATGCTGGTGAGAAAGCAGAAGACATATGCTCTATTGGATGCAGATGATGACGATGATGATGGTGATAAGTCTTCTGTTCCAGTAGTTTCCGAGTCCAGGAAAAGTGATTCCCATAAGAAACGGTTCAGGAGGAAGGCCTCGAGTCAAGACGATGAAGATGATGAGGTGATTGTACACCAGGAAGAAGTGAGGCGGGTTAAAAGACGAACTTCCCCAGATGAAGATGATGGTTCAGAGTCCGAAGAAGAAAGATTGCGTGATCAAAGAGAGCGGGAGCAATTAGAGCGCAATATAAGGGAGAGGGACACAGCAGCTACACGAAAGTTAACAGAGAGAAAGTTATCAAAAAAAGAGGAAGAGGAGGCTATTCGGAGAAACAAGGCTTCAGAGCGAAATGAAACTGAAGATTTAAGACTAGTTTCAAGACAAGAATATTTAAAGAAAAGACAGCAGAAGAAACTGGAGGAAATCAGAGATGAAATAGAAGATGAGCAATACTTATTTGAGCACGTGGAGCTCACTGAATTGGAACGTCGTGAATTAAGTTACAAGAAGCAAATACTTGAGGCTGTAACGAAGCGGGCAGTTGAGGACGAGAATCAAAATGAGTACAGGATTCCTGATGCCTATGATGTGGAGGGGGGTGTCAATCAGGAGAAGAGATTTTCTGTGGCCATGCAACGCTACAGGGACCTGGCTGGGGATAAAATGAATCCATTTGCAGAACAGGAAGCTTGGGAGGATCACCAAATTGGAAAGGCAACACTGCAGTATGGTTCAAAAAATAAAAAGCGATCTGATGAGTATCAATATGTATTTGAGGACCAGATTGATTTCATCAAGGCATCAGTAATGGATGGTGACCAGTTTGAGGATGGGCAACCGGCGGATTTACTTGAGTCGAGAGCGAAATCAGAGTTGGAGAAGCTCCAGGAGGACAGAAAAACATTACCCATATACTCCTATCGTGATGAGTTGCTCAAAGCTGTTGATAACCATCAGGTTCTTGTTATTGTTGGTGAAACCGGTTCTGGGAAAACTACGCAAATACCCCAGTATCTTCACGAGGCCGGATACACAAAACGTGGAAAGATTGGGTGCACACAGCCACGACGAGTTGCTGCTATGAGTGTTGCTGCAAGGGTTTCTCAAGAAATGGGTGTGAAACTTGGGCATGAGGTAGGTTATTCCATTCGTTTCGAGGATTGCACATCGGAAAAGACGGTTTTGAAATATATGACAGATGGGATGTTGTTGCGAGAATTCCTTGGTGAACCAGATCTGGCAGGCTACAGTGTGGTTATGGTGGATGAGGCCCATGAGAGAACACTTTCAACTGATATTCTATTTGGATTAGTAAAGGATATTGCTCGATTTCGACCTGATCTTAAACTACTTATCTCAAGTGCAACACTTGATGCTGAGAAGTTCAGTGATTATTTTGATTCTGCTCCAATTTTCAAAATTCCGGGGAGGCGTTATCCTGTTGAAATAAATTACACTAAAGCACCAGAAGCTGATTACTTGGATGCAGCAATTGTTACTGCACTGCAAATCCATGTGACAGAAGCATCTGGAGATATATTAGTGTTCCTGACAGGTCAAGAAGAAATTGAAACAGCGGAAGAAATATTGAAGCACAGAACAAGGGGCTTAGGGACAAAAATTGCCGAGCTTATTATTTGTCCCATATATGCAAACCTACCTACTGAGCTACAATCCAAAATTTTTGAGCCCACACCTGAAGGGGCAAGGAAAGTTGTCCTTGCGACAAATATAGCTGAAACTTCACTAACTATTGATGGGATCAAATATGTTATTGACCCAGGGTTTTGCAAGATGAAGTCATATAATCCAAGGACTGGGATGGAGTCGTTGCTGGTCGCTCCCATCTCAAAGGCATCAGCCAATCAGAGGGCAGGTCGATCTGGGAGAACGGGTCCTGGAAAGTGCTACCGGCTATATACTATGTTCAATTACCAAACTGAGTTAGAAGATAACACGGTACCAGAAATACAGAGGACTAACCTTGCAAATGTTGTGCTCATGCTGAAAAGCCTTGGTATCCATGACCTATTACATTTTGATTTTATGGACCCGCCACCATCAGAGGCATTACTGAAAGCCTTGGAACTGTTATTTGCACTAAGTGCATTAAATAAAGTGGGTGAGTTGACTAAAGTTGGTAGACGGATGGCCGAGTTTCCTCTTGATCCTATGCTATCTAAGATGATAGTTGCTTCTGATAAGTACAAGTGTTCAGATGAGATCATTTCCATTGCTTCCATGCTTTCCATTGGTAATTCAATATTTTACCGTCCAAAGGACAAACAAGTCCATGCTGACAATGCACGGTTGAATTTTCACACTGGGAATGTGGGAGATCACATCGCATTGCTCAAGGTTTTTAATTCATGGAAAGAGACCAATTTCTCAACACAATGGTGTTACGAGAATTATATACAGGTCAGGAGTATGAAGCGCGCTAGAGATATTAGAGACCAGCTTGAGGGACTCTTGGAGAGGGTCGAGATTGAGCAGGTCTCAAATCCTGATTTTGAAGTTATAAAGAAGGCCATTACATCTGGCTTTTTCCCTCATTCCGCAAGACTGCAAAAGAGTGGAGCTTATAGAACAGTCAAACATCCGCAGACTGTCCACATACACCCCAGCTCAGGGCTTTCTCAGGTGCTTCCAAGATGGGTTATATACCATGAGTTGGTGCTTACAACCAAGGAATATATGAGACAGGTAACGGAACTGAAGCCAGAGTGGTTGGTGGAAATAGCTCCGCATTATTACCAGCTAAAGGATGTTGAAGATTCGGTGTCAAAGAAAATGCCTCGTGGAGAAGGGCGCGCATCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009790 GO:0009791 GO:0009793 GO:0009892 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0016020 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031047 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1042

Amino Acids

119.13

Weight (kDa)

5.75

Isoelectric Point (pI)

45.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Helicase_C	PF00271	608 - 734	1.1e-11	Helicase conserved C-terminal domain
WHD_HA2	PF04408	796 - 824	1e-09	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	825 - 886	4.7e-11	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	943 - 1020	7.1e-24	Oligonucleotide/oligosaccharide-binding (OB)-fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000463)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32490 AT1G32490 AT2G35340 AT2G35340 AT4G16680 AT4G16680
fragaria_vesca	FvH4_1g06760 FvH4_1g06761 FvH4_1g06770 FvH4_3g40380 FvH4_3g40450 FvH4_5g14240 FvH4_5g14240 FvH4_5g14240 FvH4_5g14240 FvH4_7g01330 FvH4_7g01330
malus_domestica	MD02G1072400.v1.1 MD02G1072500.v1.1 MD02G1088100.v1.1 MD06G1200100.v1.1 MD07G1285300.v1.1 MD08G1042900.v1.1
prunus_persica	Prupe.5G201700_v2.0.a1 Prupe.5G201700_v2.0.a1 Prupe.6G014300_v2.0.a1 Prupe.6G159800_v2.0.a1 Prupe.7G201500_v2.0.a1 Prupe.8G103900_v2.0.a1 Prupe.8G104500_v2.0.a1
pyrus_communis	pycom02g05640 pycom02g05650 pycom14g17310
rosa_chinensis	RchiOBHm_Chr2g0092491 RchiOBHm_Chr2g0092541 RchiOBHm_Chr5g0073091 RchiOBHm_Chr6g0263651 RchiOBHm_Chr6g0263711 RchiOBHm_Chr7g0182681
rosa_laevigata	RLG00000005109 RLG00000014320 RLG00000014322 RLG00000014324 RLG00000014326 RLG00000014327 RLG00000016318 RLG00000016322 RLG00000036362
rosa_multiflora	Rmu_sc0000014.1_g000045 Rmu_sc0002174.1_g000001 Rmu_sc0003570.1_g000001 Rmu_sc0004296.1_g000005 Rmu_sc0004296.1_g000014 Rmu_sc0005592.1_g000025
rosa_roxburghii	Rroxscaffold_176G00431160 Rroxscaffold_176G00431170 Rroxscaffold_2G00148990 Rroxscaffold_2G00149040 Rroxscaffold_3G00271070 Rroxscaffold_6G00409880 Rroxscaffold_7G00204020 Rroxscaffold_7G00204040 Rroxscaffold_7G00204070
rosa_rugosa	Rorug02G0027100 Rorug02G0027200 Rorug02G0027300 Rorug05G0421000 Rorug06G0449100
rosa_samantha	Rh2AG072900 Rh2AG073200 Rh2BG073500 Rh2BG074100 Rh2CG074700 Rh2DG071600 Rh2DG072300 Rh5AG478000 Rh5BG498700 Rh5DG510800 Rh6AG125300 Rh6BG121400 Rh6CG119800 Rh7AG052900 Rh7BG052500 Rh7CG054000 Rh7DG052300
rosa_wichuraiana	Rw0G005280 Rw2G005970 Rw2G006000 Rw5G044390 Rw6G010890 Rw7G004310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2828
AasI	GACNNNNNNGTC	1 cut(s)	2594
Acc36I	ACCTGC	2 cut(s)	2171, 2791
Acc65I	GGTACC	1 cut(s)	2266
AccB1I	GGYRCC	1 cut(s)	2266
AccB7I	CCANNNNNTGG	2 cut(s)	2123, 2980
AccBSI	CCGCTC	1 cut(s)	532
AccI	GTMKAC	1 cut(s)	2449
AccII	CGCG	2 cut(s)	2745, 3120
AccIII	TCCGGA	1 cut(s)	221
AciI	CCGC	9 cut(s)	454, 532, 841, 1148, 1883, 2360, 2865, 2909, 3053
AclWI	GGATC	3 cut(s)	1011, 2075, 2468
AcoI	YGGCCR	2 cut(s)	933, 2457
AcsI	RAATTY	5 cut(s)	179, 1523, 1725, 1986, 2620
AcuI	CTGAAG	5 cut(s)	207, 630, 684, 2025, 3044
AcvI	CACGTG	1 cut(s)	772
AdeI	CACNNNGTG	1 cut(s)	2411
AfaI	GTAC	5 cut(s)	86, 435, 872, 2268, 2513
AflIII	ACRYGT	1 cut(s)	173
AgeI	ACCGGT	1 cut(s)	1286
AhlI	ACTAGT	1 cut(s)	676
AjiI	CACGTC	1 cut(s)	27
AjnI	CCWGG	6 cut(s)	356, 438, 954, 1191, 2086, 2203
AjuI	GAANNNNNNNTTGG	2 cut(s)	1163, 1195
AleI	CACNNNNGTG	1 cut(s)	2704
Alw21I	GWGCWC	4 cut(s)	771, 780, 1364, 2307
Alw26I	GTCTC	5 cut(s)	120, 2678, 2754, 2809, 3001
Alw44I	GTGCAC	1 cut(s)	1360
AlwI	GGATC	3 cut(s)	1011, 2075, 2468
AlwNI	CAGNNNCTG	1 cut(s)	1778
Ama87I	CYCGRG	1 cut(s)	400
Aor13HI	TCCGGA	1 cut(s)	221
AoxI	GGCC	6 cut(s)	396, 933, 1329, 1575, 2457, 2835
ApaLI	GTGCAC	1 cut(s)	1360
ApeKI	GCWGC	5 cut(s)	257, 572, 1382, 1397, 1793
ApoI	RAATTY	5 cut(s)	179, 1523, 1725, 1986, 2620
AsiGI	ACCGGT	1 cut(s)	1286
Asp700I	GAANNNNTTC	2 cut(s)	383, 1591
Asp718I	GGTACC	1 cut(s)	2266
AspLEI	GCGC	5 cut(s)	549, 2745, 2747, 3120, 3122
AspS9I	GGNCC	7 cut(s)	156, 224, 952, 1084, 1576, 2201, 2356
AsuC2I	CCSGG	1 cut(s)	1731
AsuHPI	GGTGA	8 cut(s)	280, 335, 439, 998, 1133, 1292, 1544, 2441
AvaI	CYCGRG	1 cut(s)	400
AvaII	GGWCC	6 cut(s)	156, 224, 952, 1084, 2201, 2356
BaeGI	GKGCMC	2 cut(s)	210, 1364
BaeI	ACNNNNGTAYC	2 cut(s)	1301, 1334
BalI	TGGCCA	1 cut(s)	935
BanI	GGYRCC	1 cut(s)	2266
BanII	GRGCYC	2 cut(s)	780, 1998
BauI	CACGAG	2 cut(s)	1325, 3105
BbrPI	CACGTG	1 cut(s)	772
BbsI	GAAGAC	3 cut(s)	29, 287, 324
Bbv12I	GWGCWC	4 cut(s)	771, 780, 1364, 2307
BbvI	GCAGC	5 cut(s)	244, 584, 1369, 1384, 1805
BciT130I	CCWGG	6 cut(s)	358, 440, 956, 1193, 2088, 2205
BciVI	GTATCC	2 cut(s)	1328, 2336
BclI	TGATCA	1 cut(s)	520
BcnI	CCSGG	1 cut(s)	1731
BcoDI	GTCTC	5 cut(s)	120, 2678, 2754, 2809, 3001
BcuI	ACTAGT	1 cut(s)	676
BfaI	CTAG	2 cut(s)	677, 2748
BfmI	CTRYAG	3 cut(s)	946, 1028, 1552
BfuAI	ACCTGC	2 cut(s)	2171, 2791
BfuI	GTATCC	2 cut(s)	1328, 2336
BglII	AGATCT	1 cut(s)	1540
BisI	GCNGC	5 cut(s)	258, 573, 1383, 1398, 1794
BlsI	GCNGC	5 cut(s)	259, 574, 1384, 1399, 1795
Bme1390I	CCNGG	7 cut(s)	358, 440, 956, 1193, 1731, 2088, 2205
Bme18I	GGWCC	6 cut(s)	156, 224, 952, 1084, 2201, 2356
BmeT110I	CYCGRG	1 cut(s)	400
BmgBI	CACGTC	1 cut(s)	27
BmgT120I	GGNCC	7 cut(s)	156, 224, 952, 1084, 1576, 2201, 2356
BmiI	GGNNCC	4 cut(s)	953, 2202, 2268, 2358
BmrFI	CCNGG	7 cut(s)	358, 440, 956, 1193, 1731, 2088, 2205
BmrI	ACTGGG	3 cut(s)	1309, 2133, 2640
BmsI	GCATC	7 cut(s)	289, 874, 1115, 1669, 1780, 1841, 2171
BmuI	ACTGGG	3 cut(s)	1309, 2133, 2640
BpiI	GAAGAC	3 cut(s)	29, 287, 324
BplI	GAGNNNNNCTC	2 cut(s)	2789, 2821
BpmI	CTGGAG	5 cut(s)	102, 184, 743, 1175, 1857
Bpu10I	CCTNAGC	2 cut(s)	1915, 2935
BpuEI	CTTGAG	6 cut(s)	845, 1178, 1395, 1648, 2645, 2789
BpuMI	CCSGG	1 cut(s)	1731
BsaAI	YACGTR	2 cut(s)	176, 772
BsaBI	GATNNNNATC	2 cut(s)	1065, 1257
BsaI	GGTCTC	3 cut(s)	2678, 2754, 2809
BsaJI	CCNNGG	8 cut(s)	1528, 1730, 2086, 2087, 2117, 2320, 2388, 2994
BsaWI	WCCGGW	2 cut(s)	221, 1286
BsaXI	ACNNNNNCTCC	2 cut(s)	2185, 2215
Bse118I	RCCGGY	4 cut(s)	70, 1144, 1286, 2217
Bse1I	ACTGG	6 cut(s)	341, 726, 1126, 1315, 2128, 2635
Bse3DI	GCAATG	4 cut(s)	50, 267, 2538, 2654
Bse8I	GATNNNNATC	2 cut(s)	1065, 1257
BseAI	TCCGGA	1 cut(s)	221
BseBI	CCWGG	6 cut(s)	358, 440, 956, 1193, 2088, 2205
BseDI	CCNNGG	8 cut(s)	1528, 1730, 2086, 2087, 2117, 2320, 2388, 2994
BseJI	GATNNNNATC	2 cut(s)	1065, 1257
BseMI	GCAATG	4 cut(s)	50, 267, 2538, 2654
BseMII	CTCAG	6 cut(s)	80, 1674, 1962, 2238, 2949, 2960
BseNI	ACTGG	6 cut(s)	341, 726, 1126, 1315, 2128, 2635
BsePI	GCGCGC	2 cut(s)	2743, 3118
BseRI	GAGGAG	2 cut(s)	151, 635
BseSI	GKGCMC	2 cut(s)	210, 1364
BseXI	GCAGC	5 cut(s)	244, 584, 1369, 1384, 1805
BseYI	CCCAGC	2 cut(s)	959, 2930
BsgI	GTGCAG	1 cut(s)	1791
Bsh1236I	CGCG	2 cut(s)	2745, 3120
BshFI	GGCC	6 cut(s)	398, 935, 1331, 1577, 2459, 2837
BshNI	GGYRCC	1 cut(s)	2266
BshTI	ACCGGT	1 cut(s)	1286
BsiHKAI	GWGCWC	4 cut(s)	771, 780, 1364, 2307
BsiHKCI	CYCGRG	1 cut(s)	400
BsiSI	CCGG	7 cut(s)	71, 222, 1145, 1287, 1332, 1730, 2218
BslFI	GGGAC	5 cut(s)	578, 965, 1932, 1933, 2787
BsmAI	GTCTC	5 cut(s)	120, 2678, 2754, 2809, 3001
BsmFI	GGGAC	5 cut(s)	578, 965, 1932, 1933, 2787
BsnI	GGCC	6 cut(s)	398, 935, 1331, 1577, 2459, 2837
Bso31I	GGTCTC	3 cut(s)	2678, 2754, 2809
BsoBI	CYCGRG	1 cut(s)	400
Bsp1286I	GDGCHC	6 cut(s)	210, 771, 780, 1364, 1998, 2307
Bsp13I	TCCGGA	1 cut(s)	221
Bsp1407I	TGTACA	1 cut(s)	433
BspACI	CCGC	9 cut(s)	454, 532, 841, 1148, 1883, 2360, 2865, 2909, 3053
BspANI	GGCC	6 cut(s)	398, 935, 1331, 1577, 2459, 2837
BspCNI	CTCAG	6 cut(s)	79, 1675, 1963, 2239, 2948, 2959
BspEI	TCCGGA	1 cut(s)	221
BspFNI	CGCG	2 cut(s)	2745, 3120
BspLI	GGNNCC	4 cut(s)	953, 2202, 2268, 2358
BspMAI	CTGCAG	1 cut(s)	1032
BspMI	ACCTGC	2 cut(s)	2171, 2791
BspPI	GGATC	3 cut(s)	1011, 2075, 2468
BspT107I	GGYRCC	1 cut(s)	2266
BspTNI	GGTCTC	3 cut(s)	2678, 2754, 2809
BsrBI	CCGCTC	1 cut(s)	532
BsrDI	GCAATG	4 cut(s)	50, 267, 2538, 2654
BsrFI	RCCGGY	4 cut(s)	70, 1144, 1286, 2217
BsrGI	TGTACA	1 cut(s)	433
BsrI	ACTGG	6 cut(s)	341, 726, 1126, 1315, 2128, 2635
BssAI	RCCGGY	4 cut(s)	70, 1144, 1286, 2217
BssECI	CCNNGG	8 cut(s)	1528, 1730, 2086, 2087, 2117, 2320, 2388, 2994
BssHII	GCGCGC	2 cut(s)	2743, 3118
BssSI	CACGAG	2 cut(s)	1325, 3105
BssT1I	CCWWGG	5 cut(s)	1528, 2117, 2320, 2388, 2994
Bst2BI	CACGAG	2 cut(s)	1325, 3105
Bst2UI	CCWGG	6 cut(s)	358, 440, 956, 1193, 2088, 2205
Bst4CI	ACNGT	9 cut(s)	384, 1486, 1556, 2266, 2397, 2579, 2616, 2899, 2917
Bst6I	CTCTTC	2 cut(s)	612, 914
BstAPI	GCANNNNNTGC	1 cut(s)	2411
BstAUI	TGTACA	1 cut(s)	433
BstBAI	YACGTR	2 cut(s)	176, 772
BstC8I	GCNNGC	5 cut(s)	72, 843, 1549, 2745, 3120
BstDEI	CTNAG	9 cut(s)	66, 1683, 1915, 1971, 2247, 2408, 2487, 2935, 2946
BstEII	GGTNACC	1 cut(s)	1121
BstFNI	CGCG	2 cut(s)	2745, 3120
BstHHI	GCGC	5 cut(s)	549, 2745, 2747, 3120, 3122
BstMAI	GTCTC	5 cut(s)	120, 2678, 2754, 2809, 3001
BstMWI	GCNNNNNNNGC	5 cut(s)	544, 775, 1775, 2411, 2609
BstNI	CCWGG	6 cut(s)	358, 440, 956, 1193, 2088, 2205
BstPI	GGTNACC	1 cut(s)	1121
BstSCI	CCNGG	7 cut(s)	356, 438, 954, 1191, 1729, 2086, 2203
BstSFI	CTRYAG	3 cut(s)	946, 1028, 1552
BstSLI	GKGCMC	2 cut(s)	210, 1364
BstUI	CGCG	2 cut(s)	2745, 3120
BstV1I	GCAGC	5 cut(s)	244, 584, 1369, 1384, 1805
BstV2I	GAAGAC	3 cut(s)	29, 287, 324
BstX2I	RGATCY	1 cut(s)	1540
BstYI	RGATCY	1 cut(s)	1540
BsuI	GTATCC	2 cut(s)	1328, 2336
BsuRI	GGCC	6 cut(s)	398, 935, 1331, 1577, 2459, 2837
BtgZI	GCGATG	1 cut(s)	2635
BtrI	CACGTC	1 cut(s)	27
BtsI	GCAGTG	2 cut(s)	1025, 1808
BtsIMutI	CAGTG	6 cut(s)	780, 1025, 1561, 1699, 1808, 2628
BveI	ACCTGC	2 cut(s)	2171, 2791
Cac8I	GCNNGC	5 cut(s)	72, 843, 1549, 2745, 3120
CaiI	CAGNNNCTG	1 cut(s)	1778
CfoI	GCGC	5 cut(s)	549, 2745, 2747, 3120, 3122
Cfr10I	RCCGGY	4 cut(s)	70, 1144, 1286, 2217
Cfr13I	GGNCC	7 cut(s)	156, 224, 952, 1084, 1576, 2201, 2356
CpoI	CGGWCCG	1 cut(s)	224
Csp6I	GTAC	5 cut(s)	85, 434, 871, 2267, 2512
CspAI	ACCGGT	1 cut(s)	1286
CspI	CGGWCCG	1 cut(s)	224
CviQI	GTAC	5 cut(s)	85, 434, 871, 2267, 2512
DdeI	CTNAG	9 cut(s)	66, 1683, 1915, 1971, 2247, 2408, 2487, 2935, 2946
DraI	TTTAAA	1 cut(s)	699
DraIII	CACNNNGTG	1 cut(s)	2411
DrdI	GACNNNNNNGTC	1 cut(s)	2594
DseDI	GACNNNNNNGTC	1 cut(s)	2594
EaeI	YGGCCR	2 cut(s)	933, 2457
Eam1104I	CTCTTC	2 cut(s)	612, 914
EarI	CTCTTC	2 cut(s)	612, 914
EciI	GGCGGA	1 cut(s)	1163
Ecl136II	GAGCTC	1 cut(s)	778
Eco130I	CCWWGG	5 cut(s)	1528, 2117, 2320, 2388, 2994
Eco147I	AGGCCT	1 cut(s)	398
Eco24I	GRGCYC	2 cut(s)	780, 1998
Eco31I	GGTCTC	3 cut(s)	2678, 2754, 2809
Eco47I	GGWCC	6 cut(s)	156, 224, 952, 1084, 2201, 2356
Eco53kI	GAGCTC	1 cut(s)	778
Eco57I	CTGAAG	5 cut(s)	207, 630, 684, 2025, 3044
Eco72I	CACGTG	1 cut(s)	772
Eco88I	CYCGRG	1 cut(s)	400
Eco91I	GGTNACC	1 cut(s)	1121
EcoICRI	GAGCTC	1 cut(s)	778
EcoO109I	RGGNCCY	2 cut(s)	952, 2201
EcoO65I	GGTNACC	1 cut(s)	1121
EcoRI	GAATTC	1 cut(s)	1523
EcoRII	CCWGG	6 cut(s)	356, 438, 954, 1191, 2086, 2203
EcoT14I	CCWWGG	5 cut(s)	1528, 2117, 2320, 2388, 2994
EcoT38I	GRGCYC	2 cut(s)	780, 1998
ErhI	CCWWGG	5 cut(s)	1528, 2117, 2320, 2388, 2994
FalI	AAGNNNNNCTT	4 cut(s)	454, 486, 2010, 2042
FaqI	GGGAC	5 cut(s)	578, 965, 1932, 1933, 2787
FauI	CCCGC	4 cut(s)	447, 525, 834, 2367
FauNDI	CATATG	1 cut(s)	286
FbaI	TGATCA	1 cut(s)	520
FblI	GTMKAC	1 cut(s)	2449
Fnu4HI	GCNGC	5 cut(s)	258, 573, 1383, 1398, 1794
FriOI	GRGCYC	2 cut(s)	780, 1998
Fsp4HI	GCNGC	5 cut(s)	258, 573, 1383, 1398, 1794
FspBI	CTAG	2 cut(s)	677, 2748
GlaI	GCGC	5 cut(s)	548, 2744, 2746, 3119, 3121
GluI	GCNGC	5 cut(s)	258, 573, 1383, 1398, 1794
GsaI	CCCAGC	2 cut(s)	963, 2934
GsuI	CTGGAG	5 cut(s)	102, 184, 743, 1175, 1857
HaeIII	GGCC	6 cut(s)	398, 935, 1331, 1577, 2459, 2837
HapII	CCGG	7 cut(s)	71, 222, 1145, 1287, 1332, 1730, 2218
HhaI	GCGC	5 cut(s)	549, 2745, 2747, 3120, 3122
Hin6I	GCGC	5 cut(s)	547, 2743, 2745, 3118, 3120
HinP1I	GCGC	5 cut(s)	547, 2743, 2745, 3118, 3120
HincII	GTYRAC	2 cut(s)	588, 2436
HindII	GTYRAC	2 cut(s)	588, 2436
HindIII	AAGCTT	2 cut(s)	137, 993
HpaI	GTTAAC	1 cut(s)	588
HpaII	CCGG	7 cut(s)	71, 222, 1145, 1287, 1332, 1730, 2218
HphI	GGTGA	8 cut(s)	280, 335, 439, 998, 1133, 1292, 1544, 2441
Hpy166II	GTNNAC	7 cut(s)	436, 588, 1362, 1535, 2436, 2450, 2920
Hpy8I	GTNNAC	7 cut(s)	436, 588, 1362, 1535, 2436, 2450, 2920
Hpy99I	CGWCG	2 cut(s)	798, 1377
HpyAV	CCTTC	4 cut(s)	388, 2000, 2827, 3107
HpyCH4III	ACNGT	9 cut(s)	384, 1486, 1556, 2266, 2397, 2579, 2616, 2899, 2917
HpyCH4IV	ACGT	5 cut(s)	26, 175, 771, 793, 1345
HpyF10VI	GCNNNNNNNGC	5 cut(s)	544, 775, 1775, 2411, 2609
HpyF3I	CTNAG	9 cut(s)	66, 1683, 1915, 1971, 2247, 2408, 2487, 2935, 2946
HpySE526I	ACGT	5 cut(s)	26, 175, 771, 793, 1345
HspAI	GCGC	5 cut(s)	547, 2743, 2745, 3118, 3120
Kpn2I	TCCGGA	1 cut(s)	221
KpnI	GGTACC	1 cut(s)	2270
KroI	GCCGGC	1 cut(s)	70
KroNI	GCCGGC	1 cut(s)	72
Ksp22I	TGATCA	1 cut(s)	520
KspAI	GTTAAC	1 cut(s)	588
LmnI	GCTCC	8 cut(s)	6, 535, 775, 1194, 1717, 2152, 2885, 3055
Lsp1109I	GCAGC	5 cut(s)	244, 584, 1369, 1384, 1805
LweI	GCATC	7 cut(s)	289, 874, 1115, 1669, 1780, 1841, 2171
MaeI	CTAG	2 cut(s)	677, 2748
MaeII	ACGT	5 cut(s)	26, 175, 771, 793, 1345
MaeIII	GTNAC	7 cut(s)	806, 832, 1121, 1801, 1822, 2708, 3013
MbiI	CCGCTC	1 cut(s)	532
MfeI	CAATTG	1 cut(s)	1797
MflI	RGATCY	1 cut(s)	1540
MhlI	GDGCHC	6 cut(s)	210, 771, 780, 1364, 1998, 2307
MlsI	TGGCCA	1 cut(s)	935
MluNI	TGGCCA	1 cut(s)	935
MlyI	GAGTC	6 cut(s)	362, 412, 506, 1169, 2141, 2769
MmeI	TCCRAC	1 cut(s)	1161
Mox20I	TGGCCA	1 cut(s)	935
MroI	TCCGGA	1 cut(s)	221
MroNI	GCCGGC	1 cut(s)	70
MroXI	GAANNNNTTC	2 cut(s)	383, 1591
MscI	TGGCCA	1 cut(s)	935
MseI	TTAA	9 cut(s)	98, 459, 587, 671, 698, 803, 1650, 2417, 2670
MslI	CAYNNNNRTG	1 cut(s)	2704
Msp20I	TGGCCA	1 cut(s)	935
MspA1I	CMGCKG	2 cut(s)	122, 1883
MspI	CCGG	7 cut(s)	71, 222, 1145, 1287, 1332, 1730, 2218
MspR9I	CCNGG	7 cut(s)	358, 440, 956, 1193, 1731, 2088, 2205
MunI	CAATTG	1 cut(s)	1797
MvaI	CCWGG	6 cut(s)	358, 440, 956, 1193, 2088, 2205
MvnI	CGCG	2 cut(s)	2745, 3120
MwoI	GCNNNNNNNGC	5 cut(s)	544, 775, 1775, 2411, 2609
NaeI	GCCGGC	1 cut(s)	72
NciI	CCSGG	1 cut(s)	1731
NdeI	CATATG	1 cut(s)	286
NgoMIV	GCCGGC	1 cut(s)	70
NlaIV	GGNNCC	4 cut(s)	953, 2202, 2268, 2358
NmeAIII	GCCGAG	2 cut(s)	1957, 2485
NmuCI	GTSAC	2 cut(s)	1121, 1822
OliI	CACNNNNGTG	1 cut(s)	2704
PaeR7I	CTCGAG	1 cut(s)	400
PasI	CCCWGGG	1 cut(s)	2087
PauI	GCGCGC	2 cut(s)	2743, 3118
PceI	AGGCCT	1 cut(s)	398
PdiI	GCCGGC	1 cut(s)	72
PdmI	GAANNNNTTC	2 cut(s)	383, 1591
PfeI	GAWTC	7 cut(s)	146, 368, 859, 877, 973, 1706, 3083
PflMI	CCANNNNNTGG	2 cut(s)	2123, 2980
PfoI	TCCNGGA	3 cut(s)	356, 1191, 2203
PinAI	ACCGGT	1 cut(s)	1286
PkrI	GCNGC	5 cut(s)	259, 574, 1384, 1399, 1795
PleI	GAGTC	6 cut(s)	361, 411, 505, 1168, 2140, 2769
PmaCI	CACGTG	1 cut(s)	772
PmlI	CACGTG	1 cut(s)	772
PpsI	GAGTC	6 cut(s)	361, 411, 505, 1168, 2140, 2769
Ppu21I	YACGTR	2 cut(s)	176, 772
PpuMI	RGGWCCY	2 cut(s)	952, 2201
PsiI	TTATAA	1 cut(s)	2828
Psp124BI	GAGCTC	1 cut(s)	780
Psp5II	RGGWCCY	2 cut(s)	952, 2201
Psp6I	CCWGG	6 cut(s)	356, 438, 954, 1191, 2086, 2203
PspCI	CACGTG	1 cut(s)	772
PspEI	GGTNACC	1 cut(s)	1121
PspFI	CCCAGC	2 cut(s)	959, 2930
PspGI	CCWGG	6 cut(s)	356, 438, 954, 1191, 2086, 2203
PspN4I	GGNNCC	4 cut(s)	953, 2202, 2268, 2358
PspPI	GGNCC	7 cut(s)	156, 224, 952, 1084, 1576, 2201, 2356
PspPPI	RGGWCCY	2 cut(s)	952, 2201
PspXI	VCTCGAGB	1 cut(s)	400
PstI	CTGCAG	1 cut(s)	1032
PstNI	CAGNNNCTG	1 cut(s)	1778
PsuI	RGATCY	1 cut(s)	1540
PteI	GCGCGC	2 cut(s)	2743, 3118
PvuII	CAGCTG	1 cut(s)	122
RsaI	GTAC	5 cut(s)	86, 435, 872, 2268, 2513
RsaNI	GTAC	5 cut(s)	85, 434, 871, 2267, 2512
RseI	CAYNNNNRTG	1 cut(s)	2704
Rsr2I	CGGWCCG	1 cut(s)	224
RsrII	CGGWCCG	1 cut(s)	224
SacI	GAGCTC	1 cut(s)	780
SaqAI	TTAA	9 cut(s)	98, 459, 587, 671, 698, 803, 1650, 2417, 2670
SatI	GCNGC	5 cut(s)	258, 573, 1383, 1398, 1794
Sau96I	GGNCC	7 cut(s)	156, 224, 952, 1084, 1576, 2201, 2356
SchI	GAGTC	6 cut(s)	362, 412, 506, 1169, 2141, 2769
ScrFI	CCNGG	7 cut(s)	358, 440, 956, 1193, 1731, 2088, 2205
SduI	GDGCHC	6 cut(s)	210, 771, 780, 1364, 1998, 2307
SfaNI	GCATC	7 cut(s)	289, 874, 1115, 1669, 1780, 1841, 2171
SfcI	CTRYAG	3 cut(s)	946, 1028, 1552
Sfr274I	CTCGAG	1 cut(s)	400
SinI	GGWCC	6 cut(s)	156, 224, 952, 1084, 2201, 2356
SlaI	CTCGAG	1 cut(s)	400
SmiMI	CAYNNNNRTG	1 cut(s)	2704
SmlI	CTYRAG	7 cut(s)	400, 824, 1157, 1410, 1663, 2660, 2768
SmoI	CTYRAG	7 cut(s)	400, 824, 1157, 1410, 1663, 2660, 2768
SpeI	ACTAGT	1 cut(s)	676
SseBI	AGGCCT	1 cut(s)	398
SsiI	CCGC	9 cut(s)	454, 532, 841, 1148, 1883, 2360, 2865, 2909, 3053
SspI	AATATT	4 cut(s)	195, 695, 1893, 2571
SspMI	CTAG	2 cut(s)	677, 2748
SstI	GAGCTC	1 cut(s)	780
StuI	AGGCCT	1 cut(s)	398
StyD4I	CCNGG	7 cut(s)	356, 438, 954, 1191, 1729, 2086, 2203
StyI	CCWWGG	5 cut(s)	1528, 2117, 2320, 2388, 2994
TaaI	ACNGT	9 cut(s)	384, 1486, 1556, 2266, 2397, 2579, 2616, 2899, 2917
TaiI	ACGT	5 cut(s)	29, 178, 774, 796, 1348
TaqI	TCGA	7 cut(s)	401, 1163, 1461, 1633, 1639, 2185, 2790
TaqII	GACCGA	1 cut(s)	241
TatI	WGTACW	4 cut(s)	84, 433, 870, 2511
TfiI	GAWTC	7 cut(s)	146, 368, 859, 877, 973, 1706, 3083
Tru1I	TTAA	9 cut(s)	98, 459, 587, 671, 698, 803, 1650, 2417, 2670
Tru9I	TTAA	9 cut(s)	98, 459, 587, 671, 698, 803, 1650, 2417, 2670
TscAI	CASTG	6 cut(s)	787, 1032, 1561, 1699, 1815, 2635
TseFI	GTSAC	2 cut(s)	1121, 1822
TseI	GCWGC	5 cut(s)	257, 572, 1382, 1397, 1793
Tsp45I	GTSAC	2 cut(s)	1121, 1822
TspDTI	ATGAA	9 cut(s)	429, 495, 672, 753, 986, 1087, 2117, 2664, 2753
TspGWI	ACGGA	2 cut(s)	2467, 3032
TspRI	CASTG	6 cut(s)	787, 1032, 1561, 1699, 1815, 2635
Van91I	CCANNNNNTGG	2 cut(s)	2123, 2980
VneI	GTGCAC	1 cut(s)	1360
VpaK11BI	GGWCC	6 cut(s)	156, 224, 952, 1084, 2201, 2356
XapI	RAATTY	5 cut(s)	179, 1523, 1725, 1986, 2620
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1133
XhoI	CTCGAG	1 cut(s)	400
XmiI	GTMKAC	1 cut(s)	2449
XmnI	GAANNNNTTC	2 cut(s)	383, 1591
XspI	CTAG	2 cut(s)	677, 2748

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.