Pre-mRNA-splicing factor ATP-dependent RNA helicase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 962 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 491, 1351 |
| AccII | CGCG | 2 cut(s) | 1491, 1493 |
| AciI | CCGC | 5 cut(s) | 586, 827, 971, 1236, 1424 |
| AclWI | GGATC | 1 cut(s) | 1264 |
| AcsI | RAATTY | 2 cut(s) | 192, 988 |
| AcuI | CTGAAG | 2 cut(s) | 701, 1460 |
| AdeI | CACNNNGTG | 1 cut(s) | 779 |
| AfaI | GTAC | 3 cut(s) | 636, 881, 1356 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 491, 952, 1138, 1307, 1351 |
| AgeI | ACCGGT | 1 cut(s) | 1504 |
| AgsI | TTSAA | 8 cut(s) | 131, 190, 218, 281, 936, 1176, 1247, 1451 |
| AjnI | CCWGG | 5 cut(s) | 196, 301, 571, 756, 1043 |
| AluBI | AGCT | 8 cut(s) | 245, 403, 442, 1041, 1135, 1168, 1243, 1305 |
| AluI | AGCT | 8 cut(s) | 245, 403, 442, 1041, 1135, 1168, 1243, 1305 |
| Alw26I | GTCTC | 6 cut(s) | 196, 338, 1046, 1122, 1187, 1372 |
| AlwI | GGATC | 1 cut(s) | 1264 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 245, 572 |
| AoxI | GGCC | 1 cut(s) | 1206 |
| ApeKI | GCWGC | 2 cut(s) | 260, 442 |
| ApoI | RAATTY | 2 cut(s) | 192, 988 |
| AsiGI | ACCGGT | 1 cut(s) | 1504 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 21 |
| AspLEI | GCGC | 3 cut(s) | 1491, 1493, 1495 |
| AspS9I | GGNCC | 3 cut(s) | 569, 724, 1502 |
| AsuHPI | GGTGA | 3 cut(s) | 809, 1108, 1396 |
| AsuII | TTCGAA | 1 cut(s) | 57 |
| AvaII | GGWCC | 3 cut(s) | 569, 724, 1502 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 261, 293 |
| BauI | CACGAG | 1 cut(s) | 1476 |
| BbvI | GCAGC | 2 cut(s) | 272, 429 |
| BccI | CCATC | 6 cut(s) | 61, 490, 742, 817, 1326, 1381 |
| BciT130I | CCWGG | 5 cut(s) | 198, 303, 573, 758, 1045 |
| BciVI | GTATCC | 1 cut(s) | 219 |
| BcoDI | GTCTC | 6 cut(s) | 196, 338, 1046, 1122, 1187, 1372 |
| BfaI | CTAG | 2 cut(s) | 383, 1116 |
| BfmI | CTRYAG | 1 cut(s) | 1280 |
| BfuI | GTATCC | 1 cut(s) | 219 |
| BisI | GCNGC | 2 cut(s) | 261, 443 |
| BlpI | GCTNAGC | 1 cut(s) | 399 |
| BlsI | GCNGC | 2 cut(s) | 262, 444 |
| Bme1390I | CCNGG | 5 cut(s) | 198, 303, 573, 758, 1045 |
| Bme18I | GGWCC | 3 cut(s) | 569, 724, 1502 |
| BmgT120I | GGNCC | 3 cut(s) | 569, 724, 1502 |
| BmiI | GGNNCC | 1 cut(s) | 726 |
| BmrFI | CCNGG | 5 cut(s) | 198, 303, 573, 758, 1045 |
| BmrI | ACTGGG | 2 cut(s) | 501, 1063 |
| BmsI | GCATC | 3 cut(s) | 135, 247, 539 |
| BmuI | ACTGGG | 2 cut(s) | 501, 1063 |
| BpmI | CTGGAG | 1 cut(s) | 324 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1306 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 399 |
| Bpu14I | TTCGAA | 1 cut(s) | 57 |
| BpuEI | CTTGAG | 2 cut(s) | 1013, 1157 |
| BsaI | GGTCTC | 2 cut(s) | 1046, 1122 |
| BsaJI | CCNNGG | 6 cut(s) | 197, 485, 688, 756, 1365, 1531 |
| BsaWI | WCCGGW | 1 cut(s) | 1504 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 491, 952, 1138, 1307, 1351 |
| Bse118I | RCCGGY | 1 cut(s) | 1504 |
| Bse1I | ACTGG | 3 cut(s) | 331, 496, 1069 |
| Bse3DI | GCAATG | 5 cut(s) | 543, 743, 921, 1017, 1022 |
| BseBI | CCWGG | 5 cut(s) | 198, 303, 573, 758, 1045 |
| BseDI | CCNNGG | 6 cut(s) | 197, 485, 688, 756, 1365, 1531 |
| BseGI | GGATG | 3 cut(s) | 501, 828, 1541 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 491, 952, 1138, 1307, 1351 |
| BseMI | GCAATG | 5 cut(s) | 543, 743, 921, 1017, 1022 |
| BseMII | CTCAG | 5 cut(s) | 140, 390, 429, 1320, 1514 |
| BseNI | ACTGG | 3 cut(s) | 331, 496, 1069 |
| BsePI | GCGCGC | 2 cut(s) | 1489, 1491 |
| BseXI | GCAGC | 2 cut(s) | 272, 429 |
| BsgI | GTGCAG | 2 cut(s) | 258, 665 |
| Bsh1236I | CGCG | 2 cut(s) | 1491, 1493 |
| Bsh1285I | CGRYCG | 1 cut(s) | 986 |
| BshFI | GGCC | 1 cut(s) | 1208 |
| BshTI | ACCGGT | 1 cut(s) | 1504 |
| BsiEI | CGRYCG | 1 cut(s) | 986 |
| BsiSI | CCGG | 2 cut(s) | 1400, 1505 |
| BslFI | GGGAC | 3 cut(s) | 399, 400, 1155 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 491, 952, 1138, 1307, 1351 |
| BsmAI | GTCTC | 6 cut(s) | 196, 338, 1046, 1122, 1187, 1372 |
| BsmFI | GGGAC | 3 cut(s) | 399, 400, 1155 |
| BsnI | GGCC | 1 cut(s) | 1208 |
| Bso31I | GGTCTC | 2 cut(s) | 1046, 1122 |
| Bsp119I | TTCGAA | 1 cut(s) | 57 |
| Bsp143I | GATC | 5 cut(s) | 111, 554, 983, 1012, 1256 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 399 |
| BspACI | CCGC | 5 cut(s) | 586, 827, 971, 1236, 1424 |
| BspANI | GGCC | 1 cut(s) | 1208 |
| BspCNI | CTCAG | 5 cut(s) | 141, 391, 430, 1319, 1515 |
| BspFNI | CGCG | 2 cut(s) | 1491, 1493 |
| BspHI | TCATGA | 1 cut(s) | 697 |
| BspLI | GGNNCC | 1 cut(s) | 726 |
| BspMAI | CTGCAG | 1 cut(s) | 1284 |
| BspPI | GGATC | 1 cut(s) | 1264 |
| BspQI | GCTCTTC | 1 cut(s) | 677 |
| BspT104I | TTCGAA | 1 cut(s) | 57 |
| BspTNI | GGTCTC | 2 cut(s) | 1046, 1122 |
| BsrDI | GCAATG | 5 cut(s) | 543, 743, 921, 1017, 1022 |
| BsrFI | RCCGGY | 1 cut(s) | 1504 |
| BsrI | ACTGG | 3 cut(s) | 331, 496, 1069 |
| BssAI | RCCGGY | 1 cut(s) | 1504 |
| BssECI | CCNNGG | 6 cut(s) | 197, 485, 688, 756, 1365, 1531 |
| BssHII | GCGCGC | 2 cut(s) | 1489, 1491 |
| BssMI | GATC | 5 cut(s) | 111, 554, 983, 1012, 1256 |
| BssSI | CACGAG | 1 cut(s) | 1476 |
| BssT1I | CCWWGG | 3 cut(s) | 485, 688, 1365 |
| Bst2BI | CACGAG | 1 cut(s) | 1476 |
| Bst2UI | CCWGG | 5 cut(s) | 198, 303, 573, 758, 1045 |
| Bst4CI | ACNGT | 7 cut(s) | 161, 207, 634, 947, 1270, 1288, 1547 |
| Bst6I | CTCTTC | 1 cut(s) | 677 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 779 |
| BstBI | TTCGAA | 1 cut(s) | 57 |
| BstC8I | GCNNGC | 3 cut(s) | 1113, 1491, 1493 |
| BstDEI | CTNAG | 8 cut(s) | 149, 253, 399, 438, 776, 855, 1306, 1523 |
| BstF5I | GGATG | 3 cut(s) | 501, 828, 1541 |
| BstFNI | CGCG | 2 cut(s) | 1491, 1493 |
| BstHHI | GCGC | 3 cut(s) | 1491, 1493, 1495 |
| BstKTI | GATC | 5 cut(s) | 114, 557, 986, 1015, 1259 |
| BstMAI | GTCTC | 6 cut(s) | 196, 338, 1046, 1122, 1187, 1372 |
| BstMBI | GATC | 5 cut(s) | 111, 554, 983, 1012, 1256 |
| BstMCI | CGRYCG | 1 cut(s) | 986 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 779, 977 |
| BstNI | CCWGG | 5 cut(s) | 198, 303, 573, 758, 1045 |
| BstSCI | CCNGG | 5 cut(s) | 196, 301, 571, 756, 1043 |
| BstSFI | CTRYAG | 1 cut(s) | 1280 |
| BstUI | CGCG | 2 cut(s) | 1491, 1493 |
| BstV1I | GCAGC | 2 cut(s) | 272, 429 |
| BstX2I | RGATCY | 1 cut(s) | 1256 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 13 |
| BstYI | RGATCY | 1 cut(s) | 1256 |
| BsuI | GTATCC | 1 cut(s) | 219 |
| BsuRI | GGCC | 1 cut(s) | 1208 |
| BtsCI | GGATG | 3 cut(s) | 501, 828, 1541 |
| BtsIMutI | CAGTG | 4 cut(s) | 166, 324, 1076, 1416 |
| Cac8I | GCNNGC | 3 cut(s) | 1113, 1491, 1493 |
| CaiI | CAGNNNCTG | 2 cut(s) | 245, 572 |
| CciI | TCATGA | 1 cut(s) | 697 |
| CfoI | GCGC | 3 cut(s) | 1491, 1493, 1495 |
| Cfr10I | RCCGGY | 1 cut(s) | 1504 |
| Cfr13I | GGNCC | 3 cut(s) | 569, 724, 1502 |
| Csp6I | GTAC | 3 cut(s) | 635, 880, 1355 |
| CspAI | ACCGGT | 1 cut(s) | 1504 |
| CviAII | CATG | 7 cut(s) | 109, 287, 698, 847, 968, 1105, 1346 |
| CviQI | GTAC | 3 cut(s) | 635, 880, 1355 |
| DdeI | CTNAG | 8 cut(s) | 149, 253, 399, 438, 776, 855, 1306, 1523 |
| DpnI | GATC | 5 cut(s) | 113, 556, 985, 1014, 1258 |
| DpnII | GATC | 5 cut(s) | 111, 554, 983, 1012, 1256 |
| DraIII | CACNNNGTG | 1 cut(s) | 779 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 962 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 962 |
| Eam1104I | CTCTTC | 1 cut(s) | 677 |
| EarI | CTCTTC | 1 cut(s) | 677 |
| EciI | GGCGGA | 1 cut(s) | 842 |
| Eco130I | CCWWGG | 3 cut(s) | 485, 688, 1365 |
| Eco31I | GGTCTC | 2 cut(s) | 1046, 1122 |
| Eco47I | GGWCC | 3 cut(s) | 569, 724, 1502 |
| Eco57I | CTGAAG | 2 cut(s) | 701, 1460 |
| EcoO109I | RGGNCCY | 1 cut(s) | 569 |
| EcoRII | CCWGG | 5 cut(s) | 196, 301, 571, 756, 1043 |
| EcoT14I | CCWWGG | 3 cut(s) | 485, 688, 1365 |
| ErhI | CCWWGG | 3 cut(s) | 485, 688, 1365 |
| FaeI | CATG | 7 cut(s) | 112, 290, 701, 850, 971, 1108, 1349 |
| FaqI | GGGAC | 3 cut(s) | 399, 400, 1155 |
| FatI | CATG | 7 cut(s) | 108, 286, 697, 846, 967, 1104, 1345 |
| Fnu4HI | GCNGC | 2 cut(s) | 261, 443 |
| FokI | GGATG | 3 cut(s) | 508, 835, 1548 |
| Fsp4HI | GCNGC | 2 cut(s) | 261, 443 |
| FspBI | CTAG | 2 cut(s) | 383, 1116 |
| GlaI | GCGC | 3 cut(s) | 1490, 1492, 1494 |
| GluI | GCNGC | 2 cut(s) | 261, 443 |
| GsuI | CTGGAG | 1 cut(s) | 324 |
| HaeIII | GGCC | 1 cut(s) | 1208 |
| HapII | CCGG | 2 cut(s) | 1400, 1505 |
| HhaI | GCGC | 3 cut(s) | 1491, 1493, 1495 |
| Hin1II | CATG | 7 cut(s) | 112, 290, 701, 850, 971, 1108, 1349 |
| Hin6I | GCGC | 3 cut(s) | 1489, 1491, 1493 |
| HinP1I | GCGC | 3 cut(s) | 1489, 1491, 1493 |
| HincII | GTYRAC | 1 cut(s) | 804 |
| HindII | GTYRAC | 1 cut(s) | 804 |
| HindIII | AAGCTT | 1 cut(s) | 1241 |
| HinfI | GANTC | 7 cut(s) | 33, 173, 500, 1099, 1143, 1454, 1527 |
| HpaII | CCGG | 2 cut(s) | 1400, 1505 |
| HphI | GGTGA | 3 cut(s) | 809, 1108, 1396 |
| Hpy166II | GTNNAC | 1 cut(s) | 804 |
| Hpy188I | TCNGA | 5 cut(s) | 66, 874, 892, 1329, 1532 |
| Hpy188III | TCNNGA | 2 cut(s) | 332, 698 |
| Hpy8I | GTNNAC | 1 cut(s) | 804 |
| HpyAV | CCTTC | 6 cut(s) | 542, 813, 1198, 1242, 1478, 1493 |
| HpyCH4III | ACNGT | 7 cut(s) | 161, 207, 634, 947, 1270, 1288, 1547 |
| HpyCH4IV | ACGT | 1 cut(s) | 1460 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 779, 977 |
| HpyF3I | CTNAG | 8 cut(s) | 149, 253, 399, 438, 776, 855, 1306, 1523 |
| HpySE526I | ACGT | 1 cut(s) | 1460 |
| Hsp92II | CATG | 7 cut(s) | 112, 290, 701, 850, 971, 1108, 1349 |
| HspAI | GCGC | 3 cut(s) | 1489, 1491, 1493 |
| Kzo9I | GATC | 5 cut(s) | 111, 554, 983, 1012, 1256 |
| LguI | GCTCTTC | 1 cut(s) | 677 |
| LmnI | GCTCC | 3 cut(s) | 184, 1046, 1426 |
| Lsp1109I | GCAGC | 2 cut(s) | 272, 429 |
| LweI | GCATC | 3 cut(s) | 135, 247, 539 |
| MaeI | CTAG | 2 cut(s) | 383, 1116 |
| MaeII | ACGT | 1 cut(s) | 1460 |
| MaeIII | GTNAC | 7 cut(s) | 155, 268, 289, 705, 1076, 1314, 1552 |
| MalI | GATC | 5 cut(s) | 113, 556, 985, 1014, 1258 |
| MboI | GATC | 5 cut(s) | 111, 554, 983, 1012, 1256 |
| MboII | GAAGA | 7 cut(s) | 46, 347, 365, 694, 1216, 1453, 1463 |
| MfeI | CAATTG | 1 cut(s) | 264 |
| MflI | RGATCY | 1 cut(s) | 1256 |
| MlyI | GAGTC | 4 cut(s) | 27, 509, 1108, 1137 |
| MroXI | GAANNNNTTC | 1 cut(s) | 21 |
| MseI | TTAA | 3 cut(s) | 116, 785, 1394 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1094 |
| MspI | CCGG | 2 cut(s) | 1400, 1505 |
| MspR9I | CCNGG | 5 cut(s) | 198, 303, 573, 758, 1045 |
| MunI | CAATTG | 1 cut(s) | 264 |
| MvaI | CCWGG | 5 cut(s) | 198, 303, 573, 758, 1045 |
| MvnI | CGCG | 2 cut(s) | 1491, 1493 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 779, 977 |
| NdeII | GATC | 5 cut(s) | 111, 554, 983, 1012, 1256 |
| NlaIII | CATG | 7 cut(s) | 112, 290, 701, 850, 971, 1108, 1349 |
| NlaIV | GGNNCC | 1 cut(s) | 726 |
| NmuCI | GTSAC | 3 cut(s) | 155, 289, 1314 |
| NspV | TTCGAA | 1 cut(s) | 57 |
| PagI | TCATGA | 1 cut(s) | 697 |
| PauI | GCGCGC | 2 cut(s) | 1489, 1491 |
| PciSI | GCTCTTC | 1 cut(s) | 677 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 559, 1108 |
| PdmI | GAANNNNTTC | 1 cut(s) | 21 |
| PfeI | GAWTC | 3 cut(s) | 173, 1454, 1527 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 491, 1351 |
| PfoI | TCCNGGA | 2 cut(s) | 571, 1043 |
| PinAI | ACCGGT | 1 cut(s) | 1504 |
| PkrI | GCNGC | 2 cut(s) | 262, 444 |
| Ple19I | CGATCG | 1 cut(s) | 986 |
| PleI | GAGTC | 4 cut(s) | 27, 508, 1107, 1137 |
| PpsI | GAGTC | 4 cut(s) | 27, 508, 1107, 1137 |
| PpuMI | RGGWCCY | 1 cut(s) | 569 |
| Psp5II | RGGWCCY | 1 cut(s) | 569 |
| Psp6I | CCWGG | 5 cut(s) | 196, 301, 571, 756, 1043 |
| PspGI | CCWGG | 5 cut(s) | 196, 301, 571, 756, 1043 |
| PspN4I | GGNNCC | 1 cut(s) | 726 |
| PspPI | GGNCC | 3 cut(s) | 569, 724, 1502 |
| PspPPI | RGGWCCY | 1 cut(s) | 569 |
| PstI | CTGCAG | 1 cut(s) | 1284 |
| PstNI | CAGNNNCTG | 2 cut(s) | 245, 572 |
| PsuI | RGATCY | 1 cut(s) | 1256 |
| PteI | GCGCGC | 2 cut(s) | 1489, 1491 |
| PvuI | CGATCG | 1 cut(s) | 986 |
| RsaI | GTAC | 3 cut(s) | 636, 881, 1356 |
| RsaNI | GTAC | 3 cut(s) | 635, 880, 1355 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1094 |
| SapI | GCTCTTC | 1 cut(s) | 677 |
| SaqAI | TTAA | 3 cut(s) | 116, 785, 1394 |
| SatI | GCNGC | 2 cut(s) | 261, 443 |
| Sau3AI | GATC | 5 cut(s) | 111, 554, 983, 1012, 1256 |
| Sau96I | GGNCC | 3 cut(s) | 569, 724, 1502 |
| SchI | GAGTC | 4 cut(s) | 27, 509, 1108, 1137 |
| ScrFI | CCNGG | 5 cut(s) | 198, 303, 573, 758, 1045 |
| SfaNI | GCATC | 3 cut(s) | 135, 247, 539 |
| SfcI | CTRYAG | 1 cut(s) | 1280 |
| SfuI | TTCGAA | 1 cut(s) | 57 |
| SinI | GGWCC | 3 cut(s) | 569, 724, 1502 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1094 |
| SmlI | CTYRAG | 2 cut(s) | 1028, 1136 |
| SmoI | CTYRAG | 2 cut(s) | 1028, 1136 |
| SsiI | CCGC | 5 cut(s) | 586, 827, 971, 1236, 1424 |
| SspMI | CTAG | 2 cut(s) | 383, 1116 |
| StyD4I | CCNGG | 5 cut(s) | 196, 301, 571, 756, 1043 |
| StyI | CCWWGG | 3 cut(s) | 485, 688, 1365 |
| TaaI | ACNGT | 7 cut(s) | 161, 207, 634, 947, 1270, 1288, 1547 |
| TaiI | ACGT | 1 cut(s) | 1463 |
| TaqI | TCGA | 6 cut(s) | 57, 553, 562, 817, 986, 1516 |
| TatI | WGTACW | 1 cut(s) | 879 |
| TfiI | GAWTC | 3 cut(s) | 173, 1454, 1527 |
| Tru1I | TTAA | 3 cut(s) | 116, 785, 1394 |
| Tru9I | TTAA | 3 cut(s) | 116, 785, 1394 |
| TscAI | CASTG | 4 cut(s) | 166, 331, 1076, 1416 |
| TseFI | GTSAC | 3 cut(s) | 155, 289, 1314 |
| TseI | GCWGC | 2 cut(s) | 260, 442 |
| Tsp45I | GTSAC | 3 cut(s) | 155, 289, 1314 |
| TspDTI | ATGAA | 5 cut(s) | 377, 485, 686, 1121, 1362 |
| TspRI | CASTG | 4 cut(s) | 166, 331, 1076, 1416 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 491, 1351 |
| VpaK11BI | GGWCC | 3 cut(s) | 569, 724, 1502 |
| XapI | RAATTY | 2 cut(s) | 192, 988 |
| XmnI | GAANNNNTTC | 1 cut(s) | 21 |
| XspI | CTAG | 2 cut(s) | 383, 1116 |