Pre-mRNA-splicing factor ATP-dependent RNA helicase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 1202 |
| AccB1I | GGYRCC | 1 cut(s) | 1202 |
| AccBSI | CCGCTC | 1 cut(s) | 1054 |
| AccII | CGCG | 3 cut(s) | 685, 973, 1063 |
| AciI | CCGC | 2 cut(s) | 1054, 1061 |
| AclWI | GGATC | 2 cut(s) | 395, 408 |
| AcsI | RAATTY | 2 cut(s) | 578, 1124 |
| AfaI | GTAC | 4 cut(s) | 366, 384, 1091, 1204 |
| AflIII | ACRYGT | 1 cut(s) | 833 |
| AgsI | TTSAA | 7 cut(s) | 250, 428, 434, 451, 532, 595, 620 |
| AjnI | CCWGG | 2 cut(s) | 648, 890 |
| AluBI | AGCT | 4 cut(s) | 108, 152, 271, 734 |
| AluI | AGCT | 4 cut(s) | 108, 152, 271, 734 |
| Alw21I | GWGCWC | 1 cut(s) | 184 |
| Alw26I | GTCTC | 1 cut(s) | 801 |
| Alw44I | GTGCAC | 1 cut(s) | 180 |
| AlwI | GGATC | 2 cut(s) | 395, 408 |
| AoxI | GGCC | 1 cut(s) | 64 |
| ApaLI | GTGCAC | 1 cut(s) | 180 |
| ApeKI | GCWGC | 2 cut(s) | 731, 734 |
| ApoI | RAATTY | 2 cut(s) | 578, 1124 |
| AseI | ATTAAT | 1 cut(s) | 716 |
| Asp718I | GGTACC | 1 cut(s) | 1202 |
| AspLEI | GCGC | 2 cut(s) | 687, 973 |
| AspS9I | GGNCC | 1 cut(s) | 112 |
| AsuHPI | GGTGA | 1 cut(s) | 592 |
| AvaII | GGWCC | 1 cut(s) | 112 |
| BaeGI | GKGCMC | 1 cut(s) | 184 |
| BamHI | GGATCC | 1 cut(s) | 400 |
| BanI | GGYRCC | 1 cut(s) | 1202 |
| BbsI | GAAGAC | 1 cut(s) | 856 |
| Bbv12I | GWGCWC | 1 cut(s) | 184 |
| BbvI | GCAGC | 2 cut(s) | 721, 743 |
| BccI | CCATC | 6 cut(s) | 7, 168, 431, 499, 766, 1012 |
| BceAI | ACGGC | 1 cut(s) | 531 |
| BciT130I | CCWGG | 2 cut(s) | 650, 892 |
| BclI | TGATCA | 1 cut(s) | 187 |
| BcoDI | GTCTC | 1 cut(s) | 801 |
| BfaI | CTAG | 4 cut(s) | 116, 759, 791, 960 |
| BglII | AGATCT | 1 cut(s) | 696 |
| BisI | GCNGC | 2 cut(s) | 732, 735 |
| BlpI | GCTNAGC | 1 cut(s) | 928 |
| BlsI | GCNGC | 2 cut(s) | 733, 736 |
| Bme1390I | CCNGG | 2 cut(s) | 650, 892 |
| Bme18I | GGWCC | 1 cut(s) | 112 |
| BmgT120I | GGNCC | 1 cut(s) | 112 |
| BmiI | GGNNCC | 5 cut(s) | 402, 442, 662, 906, 1204 |
| BmrFI | CCNGG | 2 cut(s) | 650, 892 |
| BmsI | GCATC | 5 cut(s) | 461, 746, 771, 877, 934 |
| BpiI | GAAGAC | 1 cut(s) | 856 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 770, 802 |
| BpmI | CTGGAG | 1 cut(s) | 402 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 928 |
| BpuEI | CTTGAG | 1 cut(s) | 903 |
| BsaBI | GATNNNNATC | 1 cut(s) | 332 |
| BsaJI | CCNNGG | 1 cut(s) | 456 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 400, 430 |
| Bse1I | ACTGG | 3 cut(s) | 284, 419, 444 |
| Bse3DI | GCAATG | 2 cut(s) | 906, 912 |
| Bse8I | GATNNNNATC | 1 cut(s) | 332 |
| BseBI | CCWGG | 2 cut(s) | 650, 892 |
| BseDI | CCNNGG | 1 cut(s) | 456 |
| BseGI | GGATG | 2 cut(s) | 179, 476 |
| BseJI | GATNNNNATC | 1 cut(s) | 332 |
| BseMI | GCAATG | 2 cut(s) | 906, 912 |
| BseMII | CTCAG | 1 cut(s) | 942 |
| BseNI | ACTGG | 3 cut(s) | 284, 419, 444 |
| BseSI | GKGCMC | 1 cut(s) | 184 |
| BseXI | GCAGC | 2 cut(s) | 721, 743 |
| Bsh1236I | CGCG | 3 cut(s) | 685, 973, 1063 |
| BshFI | GGCC | 1 cut(s) | 66 |
| BshNI | GGYRCC | 1 cut(s) | 1202 |
| BsiHKAI | GWGCWC | 1 cut(s) | 184 |
| BsiWI | CGTACG | 1 cut(s) | 364 |
| BslFI | GGGAC | 1 cut(s) | 255 |
| BsmAI | GTCTC | 1 cut(s) | 801 |
| BsmFI | GGGAC | 1 cut(s) | 255 |
| BsmI | GAATGC | 1 cut(s) | 457 |
| BsnI | GGCC | 1 cut(s) | 66 |
| Bsp1286I | GDGCHC | 1 cut(s) | 184 |
| Bsp143I | GATC | 7 cut(s) | 122, 187, 322, 400, 465, 696, 1158 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 928 |
| BspACI | CCGC | 2 cut(s) | 1054, 1061 |
| BspANI | GGCC | 1 cut(s) | 66 |
| BspCNI | CTCAG | 1 cut(s) | 941 |
| BspFNI | CGCG | 3 cut(s) | 685, 973, 1063 |
| BspHI | TCATGA | 1 cut(s) | 574 |
| BspLI | GGNNCC | 5 cut(s) | 402, 442, 662, 906, 1204 |
| BspPI | GGATC | 2 cut(s) | 395, 408 |
| BspQI | GCTCTTC | 1 cut(s) | 90 |
| BspT107I | GGYRCC | 1 cut(s) | 1202 |
| BsrBI | CCGCTC | 1 cut(s) | 1054 |
| BsrDI | GCAATG | 2 cut(s) | 906, 912 |
| BsrI | ACTGG | 3 cut(s) | 284, 419, 444 |
| BssECI | CCNNGG | 1 cut(s) | 456 |
| BssMI | GATC | 7 cut(s) | 122, 187, 322, 400, 465, 696, 1158 |
| BssT1I | CCWWGG | 1 cut(s) | 456 |
| Bst2UI | CCWGG | 2 cut(s) | 650, 892 |
| Bst4CI | ACNGT | 3 cut(s) | 364, 871, 877 |
| Bst6I | CTCTTC | 2 cut(s) | 90, 997 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 887 |
| BstC8I | GCNNGC | 4 cut(s) | 138, 180, 897, 957 |
| BstDEI | CTNAG | 3 cut(s) | 212, 928, 1070 |
| BstF5I | GGATG | 2 cut(s) | 179, 476 |
| BstFNI | CGCG | 3 cut(s) | 685, 973, 1063 |
| BstHHI | GCGC | 2 cut(s) | 687, 973 |
| BstKTI | GATC | 7 cut(s) | 125, 190, 325, 403, 468, 699, 1161 |
| BstMAI | GTCTC | 1 cut(s) | 801 |
| BstMBI | GATC | 7 cut(s) | 122, 187, 322, 400, 465, 696, 1158 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 105, 887, 896, 1060 |
| BstNI | CCWGG | 2 cut(s) | 650, 892 |
| BstNSI | RCATGY | 1 cut(s) | 899 |
| BstSCI | CCNGG | 2 cut(s) | 648, 890 |
| BstSLI | GKGCMC | 1 cut(s) | 184 |
| BstUI | CGCG | 3 cut(s) | 685, 973, 1063 |
| BstV1I | GCAGC | 2 cut(s) | 721, 743 |
| BstV2I | GAAGAC | 1 cut(s) | 856 |
| BstX2I | RGATCY | 2 cut(s) | 400, 696 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 649 |
| BstYI | RGATCY | 2 cut(s) | 400, 696 |
| BsuRI | GGCC | 1 cut(s) | 66 |
| BtsCI | GGATG | 2 cut(s) | 179, 476 |
| BtsI | GCAGTG | 2 cut(s) | 72, 592 |
| BtsIMutI | CAGTG | 4 cut(s) | 72, 592, 747, 882 |
| Cac8I | GCNNGC | 4 cut(s) | 138, 180, 897, 957 |
| CciI | TCATGA | 1 cut(s) | 574 |
| CfoI | GCGC | 2 cut(s) | 687, 973 |
| Cfr13I | GGNCC | 1 cut(s) | 112 |
| CsiI | ACCWGGT | 1 cut(s) | 648 |
| Csp6I | GTAC | 4 cut(s) | 365, 383, 1090, 1203 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 61, 96, 520, 555 |
| CviAII | CATG | 7 cut(s) | 337, 575, 637, 896, 1109, 1153, 1178 |
| CviQI | GTAC | 4 cut(s) | 365, 383, 1090, 1203 |
| DdeI | CTNAG | 3 cut(s) | 212, 928, 1070 |
| DpnI | GATC | 7 cut(s) | 124, 189, 324, 402, 467, 698, 1160 |
| DpnII | GATC | 7 cut(s) | 122, 187, 322, 400, 465, 696, 1158 |
| Eam1104I | CTCTTC | 2 cut(s) | 90, 997 |
| EarI | CTCTTC | 2 cut(s) | 90, 997 |
| Eco130I | CCWWGG | 1 cut(s) | 456 |
| Eco47I | GGWCC | 1 cut(s) | 112 |
| EcoRI | GAATTC | 1 cut(s) | 578 |
| EcoRII | CCWGG | 2 cut(s) | 648, 890 |
| EcoT14I | CCWWGG | 1 cut(s) | 456 |
| EcoT22I | ATGCAT | 2 cut(s) | 1110, 1145 |
| ErhI | CCWWGG | 1 cut(s) | 456 |
| FaeI | CATG | 7 cut(s) | 340, 578, 640, 899, 1112, 1156, 1181 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1017, 1049, 1063, 1095 |
| FaqI | GGGAC | 1 cut(s) | 255 |
| FatI | CATG | 7 cut(s) | 336, 574, 636, 895, 1108, 1152, 1177 |
| FauNDI | CATATG | 1 cut(s) | 1139 |
| FbaI | TGATCA | 1 cut(s) | 187 |
| Fnu4HI | GCNGC | 2 cut(s) | 732, 735 |
| FokI | GGATG | 2 cut(s) | 186, 483 |
| Fsp4HI | GCNGC | 2 cut(s) | 732, 735 |
| FspBI | CTAG | 4 cut(s) | 116, 759, 791, 960 |
| GlaI | GCGC | 2 cut(s) | 686, 972 |
| GluI | GCNGC | 2 cut(s) | 732, 735 |
| GsuI | CTGGAG | 1 cut(s) | 402 |
| HaeIII | GGCC | 1 cut(s) | 66 |
| HhaI | GCGC | 2 cut(s) | 687, 973 |
| Hin1II | CATG | 7 cut(s) | 340, 578, 640, 899, 1112, 1156, 1181 |
| Hin6I | GCGC | 2 cut(s) | 685, 971 |
| HinP1I | GCGC | 2 cut(s) | 685, 971 |
| HindIII | AAGCTT | 1 cut(s) | 106 |
| HinfI | GANTC | 2 cut(s) | 794, 1172 |
| HphI | GGTGA | 1 cut(s) | 592 |
| Hpy166II | GTNNAC | 2 cut(s) | 182, 484 |
| Hpy188I | TCNGA | 7 cut(s) | 127, 327, 470, 567, 626, 811, 1211 |
| Hpy188III | TCNNGA | 3 cut(s) | 240, 434, 575 |
| Hpy8I | GTNNAC | 2 cut(s) | 182, 484 |
| Hpy99I | CGWCG | 2 cut(s) | 479, 517 |
| HpyAV | CCTTC | 3 cut(s) | 88, 739, 765 |
| HpyCH4III | ACNGT | 3 cut(s) | 364, 871, 877 |
| HpyCH4IV | ACGT | 5 cut(s) | 367, 381, 534, 833, 1088 |
| HpyCH4V | TGCA | 8 cut(s) | 182, 378, 737, 899, 947, 1084, 1108, 1143 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 105, 887, 896, 1060 |
| HpyF3I | CTNAG | 3 cut(s) | 212, 928, 1070 |
| HpySE526I | ACGT | 5 cut(s) | 367, 381, 534, 833, 1088 |
| Hsp92II | CATG | 7 cut(s) | 340, 578, 640, 899, 1112, 1156, 1181 |
| HspAI | GCGC | 2 cut(s) | 685, 971 |
| KpnI | GGTACC | 1 cut(s) | 1206 |
| Ksp22I | TGATCA | 1 cut(s) | 187 |
| Kzo9I | GATC | 7 cut(s) | 122, 187, 322, 400, 465, 696, 1158 |
| LguI | GCTCTTC | 1 cut(s) | 90 |
| LmnI | GCTCC | 1 cut(s) | 910 |
| Lsp1109I | GCAGC | 2 cut(s) | 721, 743 |
| LweI | GCATC | 5 cut(s) | 461, 746, 771, 877, 934 |
| MabI | ACCWGGT | 1 cut(s) | 648 |
| MaeI | CTAG | 4 cut(s) | 116, 759, 791, 960 |
| MaeII | ACGT | 5 cut(s) | 367, 381, 534, 833, 1088 |
| MaeIII | GTNAC | 3 cut(s) | 252, 836, 871 |
| MalI | GATC | 7 cut(s) | 124, 189, 324, 402, 467, 698, 1160 |
| MbiI | CCGCTC | 1 cut(s) | 1054 |
| MboI | GATC | 7 cut(s) | 122, 187, 322, 400, 465, 696, 1158 |
| MboII | GAAGA | 9 cut(s) | 32, 77, 308, 405, 416, 861, 953, 1014, 1037 |
| MflI | RGATCY | 2 cut(s) | 400, 696 |
| MhlI | GDGCHC | 1 cut(s) | 184 |
| MluCI | AATT | 9 cut(s) | 245, 278, 299, 578, 713, 717, 781, 1095, 1124 |
| MlyI | GAGTC | 2 cut(s) | 803, 1166 |
| MmeI | TCCRAC | 2 cut(s) | 378, 604 |
| MnlI | CCTC | 3 cut(s) | 214, 542, 943 |
| Mph1103I | ATGCAT | 2 cut(s) | 1110, 1145 |
| MseI | TTAA | 2 cut(s) | 258, 716 |
| MslI | CAYNNNNRTG | 1 cut(s) | 168 |
| MspA1I | CMGCKG | 1 cut(s) | 734 |
| MspR9I | CCNGG | 2 cut(s) | 650, 892 |
| Mva1269I | GAATGC | 1 cut(s) | 457 |
| MvaI | CCWGG | 2 cut(s) | 650, 892 |
| MvnI | CGCG | 3 cut(s) | 685, 973, 1063 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 105, 887, 896, 1060 |
| NdeI | CATATG | 1 cut(s) | 1139 |
| NdeII | GATC | 7 cut(s) | 122, 187, 322, 400, 465, 696, 1158 |
| NlaIII | CATG | 7 cut(s) | 340, 578, 640, 899, 1112, 1156, 1181 |
| NlaIV | GGNNCC | 5 cut(s) | 402, 442, 662, 906, 1204 |
| NmuCI | GTSAC | 2 cut(s) | 252, 836 |
| NsiI | ATGCAT | 2 cut(s) | 1110, 1145 |
| NspI | RCATGY | 1 cut(s) | 899 |
| PaeI | GCATGC | 1 cut(s) | 899 |
| PagI | TCATGA | 1 cut(s) | 574 |
| PciSI | GCTCTTC | 1 cut(s) | 90 |
| PctI | GAATGC | 1 cut(s) | 457 |
| Pfl23II | CGTACG | 1 cut(s) | 364 |
| PkrI | GCNGC | 2 cut(s) | 733, 736 |
| PleI | GAGTC | 2 cut(s) | 802, 1166 |
| PpsI | GAGTC | 2 cut(s) | 802, 1166 |
| PshBI | ATTAAT | 1 cut(s) | 716 |
| Psp6I | CCWGG | 2 cut(s) | 648, 890 |
| PspGI | CCWGG | 2 cut(s) | 648, 890 |
| PspLI | CGTACG | 1 cut(s) | 364 |
| PspN4I | GGNNCC | 5 cut(s) | 402, 442, 662, 906, 1204 |
| PspPI | GGNCC | 1 cut(s) | 112 |
| PsuI | RGATCY | 2 cut(s) | 400, 696 |
| PvuII | CAGCTG | 1 cut(s) | 734 |
| RsaI | GTAC | 4 cut(s) | 366, 384, 1091, 1204 |
| RsaNI | GTAC | 4 cut(s) | 365, 383, 1090, 1203 |
| RseI | CAYNNNNRTG | 1 cut(s) | 168 |
| SapI | GCTCTTC | 1 cut(s) | 90 |
| SaqAI | TTAA | 2 cut(s) | 258, 716 |
| SatI | GCNGC | 2 cut(s) | 732, 735 |
| Sau3AI | GATC | 7 cut(s) | 122, 187, 322, 400, 465, 696, 1158 |
| Sau96I | GGNCC | 1 cut(s) | 112 |
| SchI | GAGTC | 2 cut(s) | 803, 1166 |
| ScrFI | CCNGG | 2 cut(s) | 650, 892 |
| SduI | GDGCHC | 1 cut(s) | 184 |
| SexAI | ACCWGGT | 1 cut(s) | 648 |
| SfaNI | GCATC | 5 cut(s) | 461, 746, 771, 877, 934 |
| SinI | GGWCC | 1 cut(s) | 112 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 168 |
| SmlI | CTYRAG | 1 cut(s) | 918 |
| SmoI | CTYRAG | 1 cut(s) | 918 |
| SphI | GCATGC | 1 cut(s) | 899 |
| Sse9I | AATT | 9 cut(s) | 245, 278, 299, 578, 713, 717, 781, 1095, 1124 |
| SsiI | CCGC | 2 cut(s) | 1054, 1061 |
| SspI | AATATT | 1 cut(s) | 37 |
| SspMI | CTAG | 4 cut(s) | 116, 759, 791, 960 |
| StyD4I | CCNGG | 2 cut(s) | 648, 890 |
| StyI | CCWWGG | 1 cut(s) | 456 |
| TaaI | ACNGT | 3 cut(s) | 364, 871, 877 |
| TaiI | ACGT | 5 cut(s) | 370, 384, 537, 836, 1091 |
| TaqI | TCGA | 1 cut(s) | 393 |
| TasI | AATT | 9 cut(s) | 245, 278, 299, 578, 713, 717, 781, 1095, 1124 |
| Tru1I | TTAA | 2 cut(s) | 258, 716 |
| Tru9I | TTAA | 2 cut(s) | 258, 716 |
| TscAI | CASTG | 4 cut(s) | 79, 592, 747, 882 |
| TseFI | GTSAC | 2 cut(s) | 252, 836 |
| TseI | GCWGC | 2 cut(s) | 731, 734 |
| Tsp45I | GTSAC | 2 cut(s) | 252, 836 |
| TspDTI | ATGAA | 9 cut(s) | 152, 344, 486, 591, 901, 1053, 1107, 1126, 1141 |
| TspGWI | ACGGA | 2 cut(s) | 492, 897 |
| TspRI | CASTG | 4 cut(s) | 79, 592, 747, 882 |
| VneI | GTGCAC | 1 cut(s) | 180 |
| VpaK11BI | GGWCC | 1 cut(s) | 112 |
| VspI | ATTAAT | 1 cut(s) | 716 |
| XapI | RAATTY | 2 cut(s) | 578, 1124 |
| XceI | RCATGY | 1 cut(s) | 899 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 716 |
| XspI | CTAG | 4 cut(s) | 116, 759, 791, 960 |
| Zsp2I | ATGCAT | 2 cut(s) | 1110, 1145 |