Pre-mRNA-splicing factor ATP-dependent RNA helicase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 341 |
| AclWI | GGATC | 3 cut(s) | 198, 394, 685 |
| AcoI | YGGCCR | 2 cut(s) | 156, 285 |
| AcsI | RAATTY | 2 cut(s) | 632, 640 |
| AcuI | CTGAAG | 2 cut(s) | 314, 477 |
| AfaI | GTAC | 6 cut(s) | 119, 317, 515, 748, 846, 948 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 796, 942, 943 |
| AgsI | TTSAA | 1 cut(s) | 129 |
| AhlI | ACTAGT | 1 cut(s) | 868 |
| AjnI | CCWGG | 1 cut(s) | 230 |
| AluBI | AGCT | 6 cut(s) | 26, 104, 480, 585, 623, 884 |
| AluI | AGCT | 6 cut(s) | 26, 104, 480, 585, 623, 884 |
| Alw21I | GWGCWC | 2 cut(s) | 227, 967 |
| Alw26I | GTCTC | 2 cut(s) | 444, 597 |
| AlwI | GGATC | 3 cut(s) | 198, 394, 685 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 26 |
| Ama87I | CYCGRG | 1 cut(s) | 966 |
| AoxI | GGCC | 4 cut(s) | 156, 285, 483, 933 |
| ApeKI | GCWGC | 6 cut(s) | 17, 26, 53, 71, 148, 440 |
| ApoI | RAATTY | 2 cut(s) | 632, 640 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 443, 475 |
| AspS9I | GGNCC | 3 cut(s) | 484, 804, 934 |
| AsuHPI | GGTGA | 3 cut(s) | 409, 700, 724 |
| AvaI | CYCGRG | 1 cut(s) | 966 |
| AvaII | GGWCC | 1 cut(s) | 804 |
| BalI | TGGCCA | 2 cut(s) | 158, 287 |
| BauI | CACGAG | 1 cut(s) | 199 |
| Bbv12I | GWGCWC | 2 cut(s) | 227, 967 |
| BbvI | GCAGC | 6 cut(s) | 4, 13, 40, 58, 160, 452 |
| BccI | CCATC | 1 cut(s) | 542 |
| BciT130I | CCWGG | 1 cut(s) | 232 |
| BciVI | GTATCC | 1 cut(s) | 188 |
| BcoDI | GTCTC | 2 cut(s) | 444, 597 |
| BcuI | ACTAGT | 1 cut(s) | 868 |
| BfaI | CTAG | 5 cut(s) | 30, 215, 446, 524, 869 |
| BfmI | CTRYAG | 2 cut(s) | 18, 950 |
| BfuI | GTATCC | 1 cut(s) | 188 |
| BisI | GCNGC | 6 cut(s) | 18, 27, 54, 72, 149, 441 |
| BlsI | GCNGC | 6 cut(s) | 19, 28, 55, 73, 150, 442 |
| BmcAI | AGTACT | 1 cut(s) | 119 |
| Bme1390I | CCNGG | 1 cut(s) | 232 |
| Bme18I | GGWCC | 1 cut(s) | 804 |
| BmeT110I | CYCGRG | 1 cut(s) | 966 |
| BmgT120I | GGNCC | 3 cut(s) | 484, 804, 934 |
| BmiI | GGNNCC | 2 cut(s) | 805, 936 |
| BmrFI | CCNGG | 1 cut(s) | 232 |
| BpmI | CTGGAG | 1 cut(s) | 444 |
| BsaJI | CCNNGG | 2 cut(s) | 78, 814 |
| BsaWI | WCCGGW | 1 cut(s) | 626 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 417, 447 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 796, 942, 943 |
| Bse1I | ACTGG | 2 cut(s) | 288, 868 |
| Bse3DI | GCAATG | 2 cut(s) | 318, 717 |
| BseBI | CCWGG | 1 cut(s) | 232 |
| BseDI | CCNNGG | 2 cut(s) | 78, 814 |
| BseGI | GGATG | 1 cut(s) | 553 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 796, 942, 943 |
| BseMI | GCAATG | 2 cut(s) | 318, 717 |
| BseMII | CTCAG | 1 cut(s) | 384 |
| BseNI | ACTGG | 2 cut(s) | 288, 868 |
| BseXI | GCAGC | 6 cut(s) | 4, 13, 40, 58, 160, 452 |
| BseYI | CCCAGC | 1 cut(s) | 829 |
| BsgI | GTGCAG | 1 cut(s) | 561 |
| BshFI | GGCC | 4 cut(s) | 158, 287, 485, 935 |
| BsiHKAI | GWGCWC | 2 cut(s) | 227, 967 |
| BsiHKCI | CYCGRG | 1 cut(s) | 966 |
| BsiSI | CCGG | 1 cut(s) | 627 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 796, 942, 943 |
| BsmAI | GTCTC | 2 cut(s) | 444, 597 |
| BsnI | GGCC | 4 cut(s) | 158, 287, 485, 935 |
| BsoBI | CYCGRG | 1 cut(s) | 966 |
| Bsp1286I | GDGCHC | 2 cut(s) | 227, 967 |
| Bsp1407I | TGTACA | 1 cut(s) | 315 |
| Bsp143I | GATC | 5 cut(s) | 203, 399, 463, 567, 690 |
| Bsp19I | CCATGG | 1 cut(s) | 78 |
| BspACI | CCGC | 1 cut(s) | 341 |
| BspANI | GGCC | 4 cut(s) | 158, 287, 485, 935 |
| BspCNI | CTCAG | 1 cut(s) | 383 |
| BspLI | GGNNCC | 2 cut(s) | 805, 936 |
| BspMAI | CTGCAG | 1 cut(s) | 22 |
| BspPI | GGATC | 3 cut(s) | 198, 394, 685 |
| BspQI | GCTCTTC | 1 cut(s) | 920 |
| BsrDI | GCAATG | 2 cut(s) | 318, 717 |
| BsrGI | TGTACA | 1 cut(s) | 315 |
| BsrI | ACTGG | 2 cut(s) | 288, 868 |
| BssECI | CCNNGG | 2 cut(s) | 78, 814 |
| BssMI | GATC | 5 cut(s) | 203, 399, 463, 567, 690 |
| BssSI | CACGAG | 1 cut(s) | 199 |
| BssT1I | CCWWGG | 2 cut(s) | 78, 814 |
| Bst2BI | CACGAG | 1 cut(s) | 199 |
| Bst2UI | CCWGG | 1 cut(s) | 232 |
| Bst6I | CTCTTC | 2 cut(s) | 600, 920 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 26 |
| BstAUI | TGTACA | 1 cut(s) | 315 |
| BstC8I | GCNNGC | 1 cut(s) | 345 |
| BstDEI | CTNAG | 1 cut(s) | 370 |
| BstDSI | CCRYGG | 1 cut(s) | 78 |
| BstF5I | GGATG | 1 cut(s) | 553 |
| BstKTI | GATC | 5 cut(s) | 206, 402, 466, 570, 693 |
| BstMAI | GTCTC | 2 cut(s) | 444, 597 |
| BstMBI | GATC | 5 cut(s) | 203, 399, 463, 567, 690 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 23, 26, 80, 272, 349 |
| BstNI | CCWGG | 1 cut(s) | 232 |
| BstSCI | CCNGG | 1 cut(s) | 230 |
| BstSFI | CTRYAG | 2 cut(s) | 18, 950 |
| BstV1I | GCAGC | 6 cut(s) | 4, 13, 40, 58, 160, 452 |
| BstX2I | RGATCY | 1 cut(s) | 203 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 85 |
| BstYI | RGATCY | 1 cut(s) | 203 |
| BsuI | GTATCC | 1 cut(s) | 188 |
| BsuRI | GGCC | 4 cut(s) | 158, 287, 485, 935 |
| BtgI | CCRYGG | 1 cut(s) | 78 |
| BtsCI | GGATG | 1 cut(s) | 553 |
| BtsI | GCAGTG | 2 cut(s) | 159, 225 |
| BtsIMutI | CAGTG | 3 cut(s) | 159, 225, 414 |
| Cac8I | GCNNGC | 1 cut(s) | 345 |
| CaiI | CAGNNNCTG | 1 cut(s) | 26 |
| Cfr13I | GGNCC | 3 cut(s) | 484, 804, 934 |
| Csp6I | GTAC | 6 cut(s) | 118, 316, 514, 747, 845, 947 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 796, 831 |
| CviAII | CATG | 6 cut(s) | 79, 313, 511, 571, 736, 848 |
| CviQI | GTAC | 6 cut(s) | 118, 316, 514, 747, 845, 947 |
| DdeI | CTNAG | 1 cut(s) | 370 |
| DpnI | GATC | 5 cut(s) | 205, 401, 465, 569, 692 |
| DpnII | GATC | 5 cut(s) | 203, 399, 463, 567, 690 |
| DraI | TTTAAA | 1 cut(s) | 855 |
| EaeI | YGGCCR | 2 cut(s) | 156, 285 |
| Eam1104I | CTCTTC | 2 cut(s) | 600, 920 |
| EarI | CTCTTC | 2 cut(s) | 600, 920 |
| Eco130I | CCWWGG | 2 cut(s) | 78, 814 |
| Eco47I | GGWCC | 1 cut(s) | 804 |
| Eco57I | CTGAAG | 2 cut(s) | 314, 477 |
| Eco88I | CYCGRG | 1 cut(s) | 966 |
| EcoO109I | RGGNCCY | 1 cut(s) | 934 |
| EcoRII | CCWGG | 1 cut(s) | 230 |
| EcoT14I | CCWWGG | 2 cut(s) | 78, 814 |
| ErhI | CCWWGG | 2 cut(s) | 78, 814 |
| FaeI | CATG | 6 cut(s) | 82, 316, 514, 574, 739, 851 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 93, 125 |
| FatI | CATG | 6 cut(s) | 78, 312, 510, 570, 735, 847 |
| FauNDI | CATATG | 2 cut(s) | 64, 904 |
| Fnu4HI | GCNGC | 6 cut(s) | 18, 27, 54, 72, 149, 441 |
| FokI | GGATG | 1 cut(s) | 560 |
| Fsp4HI | GCNGC | 6 cut(s) | 18, 27, 54, 72, 149, 441 |
| FspBI | CTAG | 5 cut(s) | 30, 215, 446, 524, 869 |
| GluI | GCNGC | 6 cut(s) | 18, 27, 54, 72, 149, 441 |
| GsaI | CCCAGC | 1 cut(s) | 833 |
| GsuI | CTGGAG | 1 cut(s) | 444 |
| HaeIII | GGCC | 4 cut(s) | 158, 287, 485, 935 |
| HapII | CCGG | 1 cut(s) | 627 |
| Hin1II | CATG | 6 cut(s) | 82, 316, 514, 574, 739, 851 |
| HincII | GTYRAC | 1 cut(s) | 757 |
| HindII | GTYRAC | 1 cut(s) | 757 |
| HinfI | GANTC | 4 cut(s) | 300, 333, 363, 786 |
| HpaII | CCGG | 1 cut(s) | 627 |
| HphI | GGTGA | 3 cut(s) | 409, 700, 724 |
| Hpy166II | GTNNAC | 2 cut(s) | 757, 807 |
| Hpy188I | TCNGA | 2 cut(s) | 294, 768 |
| Hpy188III | TCNNGA | 3 cut(s) | 207, 372, 505 |
| Hpy8I | GTNNAC | 2 cut(s) | 757, 807 |
| Hpy99I | CGWCG | 1 cut(s) | 275 |
| HpyAV | CCTTC | 2 cut(s) | 45, 289 |
| HpyCH4IV | ACGT | 1 cut(s) | 270 |
| HpyCH4V | TGCA | 6 cut(s) | 20, 56, 148, 323, 542, 841 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 23, 26, 80, 272, 349 |
| HpyF3I | CTNAG | 1 cut(s) | 370 |
| HpySE526I | ACGT | 1 cut(s) | 270 |
| Hsp92II | CATG | 6 cut(s) | 82, 316, 514, 574, 739, 851 |
| Kzo9I | GATC | 5 cut(s) | 203, 399, 463, 567, 690 |
| LguI | GCTCTTC | 1 cut(s) | 920 |
| Lsp1109I | GCAGC | 6 cut(s) | 4, 13, 40, 58, 160, 452 |
| MaeI | CTAG | 5 cut(s) | 30, 215, 446, 524, 869 |
| MaeII | ACGT | 1 cut(s) | 270 |
| MaeIII | GTNAC | 2 cut(s) | 249, 859 |
| MalI | GATC | 5 cut(s) | 205, 401, 465, 569, 692 |
| MboI | GATC | 5 cut(s) | 203, 399, 463, 567, 690 |
| MboII | GAAGA | 4 cut(s) | 198, 358, 587, 937 |
| MfeI | CAATTG | 1 cut(s) | 152 |
| MflI | RGATCY | 1 cut(s) | 203 |
| MhlI | GDGCHC | 2 cut(s) | 227, 967 |
| MlsI | TGGCCA | 2 cut(s) | 158, 287 |
| MluCI | AATT | 3 cut(s) | 152, 632, 640 |
| MluNI | TGGCCA | 2 cut(s) | 158, 287 |
| MlyI | GAGTC | 3 cut(s) | 309, 327, 795 |
| MmeI | TCCRAC | 1 cut(s) | 763 |
| MnlI | CCTC | 4 cut(s) | 397, 419, 497, 538 |
| Mox20I | TGGCCA | 2 cut(s) | 158, 287 |
| MscI | TGGCCA | 2 cut(s) | 158, 287 |
| MseI | TTAA | 1 cut(s) | 854 |
| MslI | CAYNNNNRTG | 1 cut(s) | 83 |
| Msp20I | TGGCCA | 2 cut(s) | 158, 287 |
| MspA1I | CMGCKG | 1 cut(s) | 884 |
| MspI | CCGG | 1 cut(s) | 627 |
| MspR9I | CCNGG | 1 cut(s) | 232 |
| MunI | CAATTG | 1 cut(s) | 152 |
| MvaI | CCWGG | 1 cut(s) | 232 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 23, 26, 80, 272, 349 |
| NcoI | CCATGG | 1 cut(s) | 78 |
| NdeI | CATATG | 2 cut(s) | 64, 904 |
| NdeII | GATC | 5 cut(s) | 203, 399, 463, 567, 690 |
| NlaIII | CATG | 6 cut(s) | 82, 316, 514, 574, 739, 851 |
| NlaIV | GGNNCC | 2 cut(s) | 805, 936 |
| NmuCI | GTSAC | 2 cut(s) | 249, 859 |
| PciSI | GCTCTTC | 1 cut(s) | 920 |
| PfeI | GAWTC | 1 cut(s) | 363 |
| PkrI | GCNGC | 6 cut(s) | 19, 28, 55, 73, 150, 442 |
| PleI | GAGTC | 3 cut(s) | 308, 327, 794 |
| PpsI | GAGTC | 3 cut(s) | 308, 327, 794 |
| Psp6I | CCWGG | 1 cut(s) | 230 |
| PspFI | CCCAGC | 1 cut(s) | 829 |
| PspGI | CCWGG | 1 cut(s) | 230 |
| PspN4I | GGNNCC | 2 cut(s) | 805, 936 |
| PspPI | GGNCC | 3 cut(s) | 484, 804, 934 |
| PstI | CTGCAG | 1 cut(s) | 22 |
| PstNI | CAGNNNCTG | 1 cut(s) | 26 |
| PsuI | RGATCY | 1 cut(s) | 203 |
| PvuII | CAGCTG | 1 cut(s) | 884 |
| RsaI | GTAC | 6 cut(s) | 119, 317, 515, 748, 846, 948 |
| RsaNI | GTAC | 6 cut(s) | 118, 316, 514, 747, 845, 947 |
| RseI | CAYNNNNRTG | 1 cut(s) | 83 |
| SapI | GCTCTTC | 1 cut(s) | 920 |
| SaqAI | TTAA | 1 cut(s) | 854 |
| SatI | GCNGC | 6 cut(s) | 18, 27, 54, 72, 149, 441 |
| Sau3AI | GATC | 5 cut(s) | 203, 399, 463, 567, 690 |
| Sau96I | GGNCC | 3 cut(s) | 484, 804, 934 |
| ScaI | AGTACT | 1 cut(s) | 119 |
| SchI | GAGTC | 3 cut(s) | 309, 327, 795 |
| ScrFI | CCNGG | 1 cut(s) | 232 |
| SduI | GDGCHC | 2 cut(s) | 227, 967 |
| SetI | ASST | 9 cut(s) | 28, 106, 273, 399, 482, 519, 587, 625, 886 |
| SfcI | CTRYAG | 2 cut(s) | 18, 950 |
| SinI | GGWCC | 1 cut(s) | 804 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 83 |
| SpeI | ACTAGT | 1 cut(s) | 868 |
| Sse9I | AATT | 3 cut(s) | 152, 632, 640 |
| SsiI | CCGC | 1 cut(s) | 341 |
| SspI | AATATT | 1 cut(s) | 307 |
| SspMI | CTAG | 5 cut(s) | 30, 215, 446, 524, 869 |
| StyD4I | CCNGG | 1 cut(s) | 230 |
| StyI | CCWWGG | 2 cut(s) | 78, 814 |
| TaiI | ACGT | 1 cut(s) | 273 |
| TaqI | TCGA | 1 cut(s) | 184 |
| TasI | AATT | 3 cut(s) | 152, 632, 640 |
| TatI | WGTACW | 3 cut(s) | 117, 315, 844 |
| TfiI | GAWTC | 1 cut(s) | 363 |
| Tru1I | TTAA | 1 cut(s) | 854 |
| Tru9I | TTAA | 1 cut(s) | 854 |
| TscAI | CASTG | 3 cut(s) | 166, 232, 414 |
| TseFI | GTSAC | 2 cut(s) | 249, 859 |
| TseI | GCWGC | 6 cut(s) | 17, 26, 53, 71, 148, 440 |
| Tsp45I | GTSAC | 2 cut(s) | 249, 859 |
| TspDTI | ATGAA | 4 cut(s) | 82, 447, 693, 891 |
| TspRI | CASTG | 3 cut(s) | 166, 232, 414 |
| VpaK11BI | GGWCC | 1 cut(s) | 804 |
| XapI | RAATTY | 2 cut(s) | 632, 640 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 682 |
| XspI | CTAG | 5 cut(s) | 30, 215, 446, 524, 869 |
| ZrmI | AGTACT | 1 cut(s) | 119 |