Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0000463

Physical Location & Seq

Genomic Coordinates Reverse (-)

34754072 .. 34760536

Gene Structure 6465 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1285300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2346 bp

ATGCAGGAGGCTATTCGGAGAACAAATGCTTTTGAGAAAATTGACATTGAAGATTTAAGAAAAGTTTCAAGACAAGAATATCTAAAGAAAAGGGAGCAAAAGAAGTTGGAGGAAATCAGAGATGATTTAGAGGATGAGCAATACTTATTTGATGGCGTGAAACTCACTGAAGCAGAATATCGCGAATTAAGTTACAAGAAACAAATATACGAAATTGTGAGGAAGAGGTCAGATGAGCCTGAGGATGCCAATGAGTACAGGATGCCGGATGCCTATGATGACAAGAATGGTGTTAATCAGGAGAAGAGATTTTCTAGTGCTGGGGATAAAATGATCCCATTTGCAGAACAGGAAGCTTGGGAGGATCACCAAATTGGAAAGGCAACGCTGAAATTTGGTTCAAAAAATAAAAAGCAAATGTCAGATGACTATCGAATTGTATTTGAGGACCAGATTGAGTTTATCAAGGCATCGGTGATGGATGGTGATAAGTTTGATGATGATGGGGAACCCTCCGAGTTACTTGTGTCGAGGGTGAAATCAGCCTTAGAGAAGCTCCAGGAGGATAGAAAAACATTACCCATCTACACATACCGTGAATCATTGCTCGATGCTGTTGAAAAACATCAGGTCCTTATTATTGTTGGTGAAACCGGTTCTGGAAAAACTACACAGATACCCCAATATCTTCACGAGGCCGGATACACAAAGCGTGGAAAGATTGGATGCACACAACCACGGCGAGTTGCTGCTATGAGTGTTGCTGCAAGGGTTTCTCAAGAAATGGGGGTTAAACTTGGGCATGAGGTTGGTTATTCCATTCGTTTTGAGGATTGCACATCCGAAAAGACTGTTTTGAAATATATGACAGATGGAATGCTGTTGCAAGAATTCCTTGGTGAACCCGATCTGGCTAGCTACAGTGTCGTGATGGTGGACGAGGCCCATGAGCGAACACTGTCAACTGATATTCTATTTGGATTAGTAAAGGATATTGCTCGGTTTAGACCCGATCTTAAACTGCTTATCTCAAGTGCAACGCTTGATGCTGAAAAGTTCAGTGATTATTTTGATTCTGCTCCAATTTTTAAAATTCCTGGGAGGAGGTATCCTGTTGAAATACATTACACGAAAGCACCAGAAGCTGATTACTTAGATGCAGCAATTGTAACTGCACTCCAAATCCATGTGACGCAACCTCCAGGAGATATATTGGTGTTCCTCACTGGTCAAGAAGAAATCGAGACAGCAGAAGAAATAATGAAGCACAGAACACGGGGTCTAGGGACAAAAATTGCTGAGCTGATTATTTGTCCCATATATGCAAACCTACCAACTGAGCTGCAATCAAAAATTTTTGAAACCCACACACTGAAGGGGCAAGGAAGGTTATCCTTGCCACAAATATTGCAGAAACTTCGCTTACCATTGATGGGATCAAATATGACTGGTATGGAGTCGTTGTTAATCACTCCCATCTCAAAGGCATCAGCAATGCAGAGGGCAGGTCGATCTGGTCGAACGGGTCCTGGAAAGTGCTACCGCTTATATACTGCCTACAATTATTACAATGATTTGGACGACAACACAGTACCAGAAGTGCAGAGGACTAATCTCGCAAATGTTGTGCTTTCATTGAAGAGCCTTGGTATTCATGACTTGTTACATTTTGATTTTATGGACCCTCCACCATCTGAAGCATTACTAAAAGCCCTGGAACTTTTATTTGCACTAAGTGCATTAAATAAGGTGGGTGAGTTGACTAAAGTTGGTCGAAGGATGGCAGAGTTTCCACTTGACCCCATGCTATCTAAGATGATAGTTGCTTCTGATAAGTACAAGTGTTCAGATGAGATTATTTCCATTGCTGCCATGCTTTCCATTGGTAATTTCAATCTTTTACCGTCCAAAGGACAAGCAAGTCCATGCGGACAATGCACGATTGAATTTTCACATGGGGAACGTCGGAGATCACATTGCGTTGCTCAAGTTTATAATTCATGGAAAGAGACCAATTATTCAACCCAGTGGTGTTACGAGAATTATGTACAGGTGAGAAGCATGAAGCGTGCTAGAGATATTAGAGACCAGCTCGAGGGACTCTTGGAGAGGGTGGAGATTGAGCTGGTTTCAAATATGAGTGATAACGAGACTATAAAGAAGGCCATTACATCTGGTTTCTTCCCTCATTCTGCAAAGCTTCAAAAGAATGGATCTTATAGAACGGTCAAACATCCGCAGACTGTCCATATACATCCGAGCTCAGGGCTGTCACAGGTAAAGAAGAATTGCATTATCCTCGTTGCTACTTGCCATATGAATTTAGTCTTTTGGTTTGCAATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009790 GO:0009791 GO:0009793 GO:0009892 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0016020 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031047 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

782

Amino Acids

88.79

Weight (kDa)

6.13

Isoelectric Point (pI)

42.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WHD_HA2	PF04408	571 - 599	7.3e-10	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	600 - 639	2.3e-08	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	719 - 761	1.3e-07	Oligonucleotide/oligosaccharide-binding (OB)-fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000463)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32490 AT1G32490 AT2G35340 AT2G35340 AT4G16680 AT4G16680
fragaria_vesca	FvH4_1g06760 FvH4_1g06761 FvH4_1g06770 FvH4_3g40380 FvH4_3g40450 FvH4_5g14240 FvH4_5g14240 FvH4_5g14240 FvH4_5g14240 FvH4_7g01330 FvH4_7g01330
malus_domestica	MD02G1072400.v1.1 MD02G1072500.v1.1 MD02G1088100.v1.1 MD06G1200100.v1.1 MD07G1285300.v1.1 MD08G1042900.v1.1
prunus_persica	Prupe.5G201700_v2.0.a1 Prupe.5G201700_v2.0.a1 Prupe.6G014300_v2.0.a1 Prupe.6G159800_v2.0.a1 Prupe.7G201500_v2.0.a1 Prupe.8G103900_v2.0.a1 Prupe.8G104500_v2.0.a1
pyrus_communis	pycom02g05640 pycom02g05650 pycom14g17310
rosa_chinensis	RchiOBHm_Chr2g0092491 RchiOBHm_Chr2g0092541 RchiOBHm_Chr5g0073091 RchiOBHm_Chr6g0263651 RchiOBHm_Chr6g0263711 RchiOBHm_Chr7g0182681
rosa_laevigata	RLG00000005109 RLG00000014320 RLG00000014322 RLG00000014324 RLG00000014326 RLG00000014327 RLG00000016318 RLG00000016322 RLG00000036362
rosa_multiflora	Rmu_sc0000014.1_g000045 Rmu_sc0002174.1_g000001 Rmu_sc0003570.1_g000001 Rmu_sc0004296.1_g000005 Rmu_sc0004296.1_g000014 Rmu_sc0005592.1_g000025
rosa_roxburghii	Rroxscaffold_176G00431160 Rroxscaffold_176G00431170 Rroxscaffold_2G00148990 Rroxscaffold_2G00149040 Rroxscaffold_3G00271070 Rroxscaffold_6G00409880 Rroxscaffold_7G00204020 Rroxscaffold_7G00204040 Rroxscaffold_7G00204070
rosa_rugosa	Rorug02G0027100 Rorug02G0027200 Rorug02G0027300 Rorug05G0421000 Rorug06G0449100
rosa_samantha	Rh2AG072900 Rh2AG073200 Rh2BG073500 Rh2BG074100 Rh2CG074700 Rh2DG071600 Rh2DG072300 Rh5AG478000 Rh5BG498700 Rh5DG510800 Rh6AG125300 Rh6BG121400 Rh6CG119800 Rh7AG052900 Rh7BG052500 Rh7CG054000 Rh7DG052300
rosa_wichuraiana	Rw0G005280 Rw2G005970 Rw2G006000 Rw5G044390 Rw6G010890 Rw7G004310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1995
AasI	GACNNNNNNGTC	1 cut(s)	1920
Acc36I	ACCTGC	1 cut(s)	1496
AccII	CGCG	1 cut(s)	183
AciI	CCGC	3 cut(s)	1543, 1929, 2237
AclWI	GGATC	4 cut(s)	328, 372, 1444, 2221
AcsI	RAATTY	6 cut(s)	392, 890, 1092, 1353, 1946, 2320
AcuI	CTGAAG	3 cut(s)	189, 1394, 1716
AdeI	CACNNNGTG	1 cut(s)	1736
AfaI	GTAC	4 cut(s)	257, 1593, 1838, 2049
AfiI	CCNNNNNNNGG	4 cut(s)	1433, 1910, 2095, 2264
AgeI	ACCGGT	1 cut(s)	653
AjnI	CCWGG	5 cut(s)	558, 1096, 1201, 1528, 1713
Alw21I	GWGCWC	1 cut(s)	2264
Alw26I	GTCTC	4 cut(s)	1238, 2003, 2079, 2144
AlwI	GGATC	4 cut(s)	328, 372, 1444, 2221
AlwNI	CAGNNNCTG	1 cut(s)	1145
Ama87I	CYCGRG	1 cut(s)	2093
AoxI	GGCC	3 cut(s)	696, 942, 2163
ApeKI	GCWGC	5 cut(s)	749, 764, 1160, 1342, 1868
ApoI	RAATTY	6 cut(s)	392, 890, 1092, 1353, 1946, 2320
AsiGI	ACCGGT	1 cut(s)	653
Asp700I	GAANNNNTTC	1 cut(s)	64
AspS9I	GGNCC	5 cut(s)	448, 631, 943, 1526, 1681
AsuHPI	GGTGA	8 cut(s)	359, 487, 497, 547, 659, 911, 1766, 2065
AsuNHI	GCTAGC	1 cut(s)	914
AvaI	CYCGRG	1 cut(s)	2093
AvaII	GGWCC	4 cut(s)	448, 631, 1526, 1681
AxyI	CCTNAGG	1 cut(s)	240
BanII	GRGCYC	1 cut(s)	2264
BauI	CACGAG	1 cut(s)	692
Bbv12I	GWGCWC	1 cut(s)	2264
BbvI	GCAGC	5 cut(s)	736, 751, 1172, 1329, 1855
BceAI	ACGGC	1 cut(s)	755
BcgI	CGANNNNNNTGC	4 cut(s)	1400, 1434, 2281, 2315
BciT130I	CCWGG	5 cut(s)	560, 1098, 1203, 1530, 1715
BciVI	GTATCC	2 cut(s)	695, 1119
BcoDI	GTCTC	4 cut(s)	1238, 2003, 2079, 2144
BfaI	CTAG	4 cut(s)	315, 915, 1283, 2073
BfmI	CTRYAG	1 cut(s)	919
BfuAI	ACCTGC	1 cut(s)	1496
BfuI	GTATCC	2 cut(s)	695, 1119
BisI	GCNGC	5 cut(s)	750, 765, 1161, 1343, 1869
BlpI	GCTNAGC	1 cut(s)	1299
BlsI	GCNGC	5 cut(s)	751, 766, 1162, 1344, 1870
Bme1390I	CCNGG	5 cut(s)	560, 1098, 1203, 1530, 1715
Bme18I	GGWCC	4 cut(s)	448, 631, 1526, 1681
BmeT110I	CYCGRG	1 cut(s)	2093
BmgT120I	GGNCC	5 cut(s)	448, 631, 943, 1526, 1681
BmiI	GGNNCC	3 cut(s)	510, 1527, 1683
BmrFI	CCNGG	5 cut(s)	560, 1098, 1203, 1530, 1715
BmrI	ACTGGG	1 cut(s)	2020
BmsI	GCATC	9 cut(s)	235, 252, 259, 479, 601, 716, 1036, 1147, 1496
BmtI	GCTAGC	1 cut(s)	918
BmuI	ACTGGG	1 cut(s)	2020
BplI	GAGNNNNNCTC	2 cut(s)	2076, 2108
BpmI	CTGGAG	2 cut(s)	542, 1185
Bpu10I	CCTNAGC	1 cut(s)	2263
Bpu1102I	GCTNAGC	1 cut(s)	1299
BpuEI	CTTGAG	3 cut(s)	762, 1015, 1971
BsaBI	GATNNNNATC	1 cut(s)	429
BsaI	GGTCTC	2 cut(s)	2003, 2079
BsaJI	CCNNGG	5 cut(s)	737, 895, 1097, 1645, 1713
BsaWI	WCCGGW	1 cut(s)	653
BsaXI	ACNNNNNCTCC	4 cut(s)	1183, 1213, 1448, 1478
Bsc4I	CCNNNNNNNGG	4 cut(s)	1433, 1910, 2095, 2264
Bse118I	RCCGGY	1 cut(s)	653
Bse1I	ACTGG	3 cut(s)	1231, 1453, 2026
Bse21I	CCTNAGG	1 cut(s)	240
Bse3DI	GCAATG	4 cut(s)	602, 1500, 1863, 1975
Bse8I	GATNNNNATC	1 cut(s)	429
BseBI	CCWGG	5 cut(s)	560, 1098, 1203, 1530, 1715
BseDI	CCNNGG	5 cut(s)	737, 895, 1097, 1645, 1713
BseJI	GATNNNNATC	1 cut(s)	429
BseLI	CCNNNNNNNGG	4 cut(s)	1433, 1910, 2095, 2264
BseMI	GCAATG	4 cut(s)	602, 1500, 1863, 1975
BseMII	CTCAG	4 cut(s)	231, 1290, 1329, 2277
BseNI	ACTGG	3 cut(s)	1231, 1453, 2026
BseRI	GAGGAG	1 cut(s)	1117
BseXI	GCAGC	5 cut(s)	736, 751, 1172, 1329, 1855
BseYI	CCCAGC	1 cut(s)	320
BsgI	GTGCAG	2 cut(s)	1158, 1622
Bsh1236I	CGCG	1 cut(s)	183
BshFI	GGCC	3 cut(s)	698, 944, 2165
BshTI	ACCGGT	1 cut(s)	653
BsiHKAI	GWGCWC	1 cut(s)	2264
BsiHKCI	CYCGRG	1 cut(s)	2093
BsiSI	CCGG	3 cut(s)	266, 654, 699
BslFI	GGGAC	3 cut(s)	1299, 1300, 2112
BslI	CCNNNNNNNGG	4 cut(s)	1433, 1910, 2095, 2264
BsmAI	GTCTC	4 cut(s)	1238, 2003, 2079, 2144
BsmFI	GGGAC	3 cut(s)	1299, 1300, 2112
BsmI	GAATGC	1 cut(s)	882
BsnI	GGCC	3 cut(s)	698, 944, 2165
Bso31I	GGTCTC	2 cut(s)	2003, 2079
BsoBI	CYCGRG	1 cut(s)	2093
Bsp1286I	GDGCHC	1 cut(s)	2264
Bsp1407I	TGTACA	1 cut(s)	2047
Bsp143I	GATC	8 cut(s)	333, 364, 907, 1012, 1436, 1511, 1970, 2213
Bsp1720I	GCTNAGC	1 cut(s)	1299
Bsp68I	TCGCGA	1 cut(s)	183
BspACI	CCGC	3 cut(s)	1543, 1929, 2237
BspANI	GGCC	3 cut(s)	698, 944, 2165
BspCNI	CTCAG	4 cut(s)	232, 1291, 1330, 2276
BspFNI	CGCG	1 cut(s)	183
BspHI	TCATGA	1 cut(s)	1654
BspLI	GGNNCC	3 cut(s)	510, 1527, 1683
BspMI	ACCTGC	1 cut(s)	1496
BspOI	GCTAGC	1 cut(s)	918
BspPI	GGATC	4 cut(s)	328, 372, 1444, 2221
BspQI	GCTCTTC	1 cut(s)	1634
BspTNI	GGTCTC	2 cut(s)	2003, 2079
BsrDI	GCAATG	4 cut(s)	602, 1500, 1863, 1975
BsrFI	RCCGGY	1 cut(s)	653
BsrGI	TGTACA	1 cut(s)	2047
BsrI	ACTGG	3 cut(s)	1231, 1453, 2026
BssAI	RCCGGY	1 cut(s)	653
BssECI	CCNNGG	5 cut(s)	737, 895, 1097, 1645, 1713
BssMI	GATC	8 cut(s)	333, 364, 907, 1012, 1436, 1511, 1970, 2213
BssSI	CACGAG	1 cut(s)	692
BssT1I	CCWWGG	2 cut(s)	895, 1645
Bst2BI	CACGAG	1 cut(s)	692
Bst2UI	CCWGG	5 cut(s)	560, 1098, 1203, 1530, 1715
Bst4CI	ACNGT	8 cut(s)	596, 853, 923, 960, 1591, 1905, 2227, 2245
Bst6I	CTCTTC	3 cut(s)	218, 299, 1634
BstAPI	GCANNNNNTGC	1 cut(s)	1736
BstAUI	TGTACA	1 cut(s)	2047
BstC8I	GCNNGC	2 cut(s)	916, 2070
BstDEI	CTNAG	8 cut(s)	240, 547, 1153, 1299, 1338, 1733, 1812, 2263
BstDSI	CCRYGG	1 cut(s)	737
BstFNI	CGCG	1 cut(s)	183
BstKTI	GATC	8 cut(s)	336, 367, 910, 1015, 1439, 1514, 1973, 2216
BstMAI	GTCTC	4 cut(s)	1238, 2003, 2079, 2144
BstMBI	GATC	8 cut(s)	333, 364, 907, 1012, 1436, 1511, 1970, 2213
BstMWI	GCNNNNNNNGC	3 cut(s)	1142, 1736, 1935
BstNI	CCWGG	5 cut(s)	560, 1098, 1203, 1530, 1715
BstSCI	CCNGG	5 cut(s)	558, 1096, 1201, 1528, 1713
BstSFI	CTRYAG	1 cut(s)	919
BstUI	CGCG	1 cut(s)	183
BstV1I	GCAGC	5 cut(s)	736, 751, 1172, 1329, 1855
BstX2I	RGATCY	1 cut(s)	2213
BstYI	RGATCY	1 cut(s)	2213
Bsu36I	CCTNAGG	1 cut(s)	240
BsuI	GTATCC	2 cut(s)	695, 1119
BsuRI	GGCC	3 cut(s)	698, 944, 2165
BtgI	CCRYGG	1 cut(s)	737
BtsIMutI	CAGTG	7 cut(s)	165, 928, 956, 1066, 1224, 1370, 2033
BtuMI	TCGCGA	1 cut(s)	183
BveI	ACCTGC	1 cut(s)	1496
Cac8I	GCNNGC	2 cut(s)	916, 2070
CaiI	CAGNNNCTG	1 cut(s)	1145
CciI	TCATGA	1 cut(s)	1654
Cfr10I	RCCGGY	1 cut(s)	653
Cfr13I	GGNCC	5 cut(s)	448, 631, 943, 1526, 1681
CseI	GACGC	1 cut(s)	1201
Csp6I	GTAC	4 cut(s)	256, 1592, 1837, 2048
CspAI	ACCGGT	1 cut(s)	653
CspCI	CAANNNNNGTGG	2 cut(s)	1389, 1424
CviQI	GTAC	4 cut(s)	256, 1592, 1837, 2048
DdeI	CTNAG	8 cut(s)	240, 547, 1153, 1299, 1338, 1733, 1812, 2263
DpnI	GATC	8 cut(s)	335, 366, 909, 1014, 1438, 1513, 1972, 2215
DpnII	GATC	8 cut(s)	333, 364, 907, 1012, 1436, 1511, 1970, 2213
DraI	TTTAAA	1 cut(s)	1090
DraIII	CACNNNGTG	1 cut(s)	1736
DrdI	GACNNNNNNGTC	1 cut(s)	1920
DseDI	GACNNNNNNGTC	1 cut(s)	1920
Eam1104I	CTCTTC	3 cut(s)	218, 299, 1634
EarI	CTCTTC	3 cut(s)	218, 299, 1634
Ecl136II	GAGCTC	1 cut(s)	2262
Eco130I	CCWWGG	2 cut(s)	895, 1645
Eco24I	GRGCYC	1 cut(s)	2264
Eco31I	GGTCTC	2 cut(s)	2003, 2079
Eco47I	GGWCC	4 cut(s)	448, 631, 1526, 1681
Eco53kI	GAGCTC	1 cut(s)	2262
Eco57I	CTGAAG	3 cut(s)	189, 1394, 1716
Eco81I	CCTNAGG	1 cut(s)	240
Eco88I	CYCGRG	1 cut(s)	2093
EcoICRI	GAGCTC	1 cut(s)	2262
EcoO109I	RGGNCCY	2 cut(s)	631, 1526
EcoRI	GAATTC	1 cut(s)	890
EcoRII	CCWGG	5 cut(s)	558, 1096, 1201, 1528, 1713
EcoT14I	CCWWGG	2 cut(s)	895, 1645
EcoT38I	GRGCYC	1 cut(s)	2264
ErhI	CCWWGG	2 cut(s)	895, 1645
FalI	AAGNNNNNCTT	2 cut(s)	879, 911
FaqI	GGGAC	3 cut(s)	1299, 1300, 2112
FauNDI	CATATG	1 cut(s)	2316
Fnu4HI	GCNGC	5 cut(s)	750, 765, 1161, 1343, 1869
FriOI	GRGCYC	1 cut(s)	2264
Fsp4HI	GCNGC	5 cut(s)	750, 765, 1161, 1343, 1869
FspBI	CTAG	4 cut(s)	315, 915, 1283, 2073
GluI	GCNGC	5 cut(s)	750, 765, 1161, 1343, 1869
GsaI	CCCAGC	1 cut(s)	324
GsuI	CTGGAG	2 cut(s)	542, 1185
HaeIII	GGCC	3 cut(s)	698, 944, 2165
HapII	CCGG	3 cut(s)	266, 654, 699
HgaI	GACGC	1 cut(s)	1201
HincII	GTYRAC	2 cut(s)	963, 1761
HindII	GTYRAC	2 cut(s)	963, 1761
HindIII	AAGCTT	2 cut(s)	354, 2198
HinfI	GANTC	4 cut(s)	599, 1073, 1457, 2100
HpaII	CCGG	3 cut(s)	266, 654, 699
HphI	GGTGA	8 cut(s)	359, 487, 497, 547, 659, 911, 1766, 2065
Hpy166II	GTNNAC	4 cut(s)	902, 937, 963, 1761
Hpy8I	GTNNAC	4 cut(s)	902, 937, 963, 1761
Hpy99I	CGWCG	1 cut(s)	1968
HpyAV	CCTTC	4 cut(s)	1369, 1380, 1770, 2155
HpyCH4III	ACNGT	8 cut(s)	596, 853, 923, 960, 1591, 1905, 2227, 2245
HpyCH4IV	ACGT	1 cut(s)	1963
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1142, 1736, 1935
HpyF3I	CTNAG	8 cut(s)	240, 547, 1153, 1299, 1338, 1733, 1812, 2263
HpySE526I	ACGT	1 cut(s)	1963
Kzo9I	GATC	8 cut(s)	333, 364, 907, 1012, 1436, 1511, 1970, 2213
LguI	GCTCTTC	1 cut(s)	1634
LmnI	GCTCC	3 cut(s)	94, 561, 1084
Lsp1109I	GCAGC	5 cut(s)	736, 751, 1172, 1329, 1855
LweI	GCATC	9 cut(s)	235, 252, 259, 479, 601, 716, 1036, 1147, 1496
MaeI	CTAG	4 cut(s)	315, 915, 1283, 2073
MaeII	ACGT	1 cut(s)	1963
MaeIII	GTNAC	7 cut(s)	191, 519, 1168, 1189, 1662, 2033, 2271
MalI	GATC	8 cut(s)	335, 366, 909, 1014, 1438, 1513, 1972, 2215
MboI	GATC	8 cut(s)	333, 364, 907, 1012, 1436, 1511, 1970, 2213
MboII	GAAGA	9 cut(s)	62, 235, 316, 680, 1247, 1265, 1651, 2173, 2296
MfeI	CAATTG	1 cut(s)	1164
MflI	RGATCY	1 cut(s)	2213
MhlI	GDGCHC	1 cut(s)	2264
MlyI	GAGTC	2 cut(s)	1466, 2094
MmeI	TCCRAC	2 cut(s)	87, 1946
MroXI	GAANNNNTTC	1 cut(s)	64
MseI	TTAA	8 cut(s)	56, 188, 294, 792, 1017, 1089, 1466, 1742
MspI	CCGG	3 cut(s)	266, 654, 699
MspR9I	CCNGG	5 cut(s)	560, 1098, 1203, 1530, 1715
MunI	CAATTG	1 cut(s)	1164
Mva1269I	GAATGC	1 cut(s)	882
MvaI	CCWGG	5 cut(s)	560, 1098, 1203, 1530, 1715
MvnI	CGCG	1 cut(s)	183
MwoI	GCNNNNNNNGC	3 cut(s)	1142, 1736, 1935
NdeI	CATATG	1 cut(s)	2316
NdeII	GATC	8 cut(s)	333, 364, 907, 1012, 1436, 1511, 1970, 2213
NheI	GCTAGC	1 cut(s)	914
NlaIV	GGNNCC	3 cut(s)	510, 1527, 1683
NmuCI	GTSAC	2 cut(s)	1189, 2271
NruI	TCGCGA	1 cut(s)	183
PaeR7I	CTCGAG	1 cut(s)	2093
PagI	TCATGA	1 cut(s)	1654
PciSI	GCTCTTC	1 cut(s)	1634
PcsI	WCGNNNNNNNCGW	1 cut(s)	1516
PctI	GAATGC	1 cut(s)	882
PdmI	GAANNNNTTC	1 cut(s)	64
PfeI	GAWTC	2 cut(s)	599, 1073
PfoI	TCCNGGA	3 cut(s)	558, 1201, 1528
PinAI	ACCGGT	1 cut(s)	653
PkrI	GCNGC	5 cut(s)	751, 766, 1162, 1344, 1870
PleI	GAGTC	2 cut(s)	1465, 2094
PpsI	GAGTC	2 cut(s)	1465, 2094
PpuMI	RGGWCCY	2 cut(s)	631, 1526
PsiI	TTATAA	1 cut(s)	1995
Psp124BI	GAGCTC	1 cut(s)	2264
Psp5II	RGGWCCY	2 cut(s)	631, 1526
Psp6I	CCWGG	5 cut(s)	558, 1096, 1201, 1528, 1713
PspFI	CCCAGC	1 cut(s)	320
PspGI	CCWGG	5 cut(s)	558, 1096, 1201, 1528, 1713
PspN4I	GGNNCC	3 cut(s)	510, 1527, 1683
PspPI	GGNCC	5 cut(s)	448, 631, 943, 1526, 1681
PspPPI	RGGWCCY	2 cut(s)	631, 1526
PspXI	VCTCGAGB	1 cut(s)	2093
PstNI	CAGNNNCTG	1 cut(s)	1145
PsuI	RGATCY	1 cut(s)	2213
RruI	TCGCGA	1 cut(s)	183
RsaI	GTAC	4 cut(s)	257, 1593, 1838, 2049
RsaNI	GTAC	4 cut(s)	256, 1592, 1837, 2048
SacI	GAGCTC	1 cut(s)	2264
SapI	GCTCTTC	1 cut(s)	1634
SaqAI	TTAA	8 cut(s)	56, 188, 294, 792, 1017, 1089, 1466, 1742
SatI	GCNGC	5 cut(s)	750, 765, 1161, 1343, 1869
Sau3AI	GATC	8 cut(s)	333, 364, 907, 1012, 1436, 1511, 1970, 2213
Sau96I	GGNCC	5 cut(s)	448, 631, 943, 1526, 1681
SchI	GAGTC	2 cut(s)	1466, 2094
ScrFI	CCNGG	5 cut(s)	560, 1098, 1203, 1530, 1715
SduI	GDGCHC	1 cut(s)	2264
SfaNI	GCATC	9 cut(s)	235, 252, 259, 479, 601, 716, 1036, 1147, 1496
SfcI	CTRYAG	1 cut(s)	919
Sfr274I	CTCGAG	1 cut(s)	2093
SinI	GGWCC	4 cut(s)	448, 631, 1526, 1681
SlaI	CTCGAG	1 cut(s)	2093
SmlI	CTYRAG	4 cut(s)	777, 1030, 1986, 2093
SmoI	CTYRAG	4 cut(s)	777, 1030, 1986, 2093
SsiI	CCGC	3 cut(s)	1543, 1929, 2237
SspI	AATATT	1 cut(s)	1407
SspMI	CTAG	4 cut(s)	315, 915, 1283, 2073
SstI	GAGCTC	1 cut(s)	2264
StyD4I	CCNGG	5 cut(s)	558, 1096, 1201, 1528, 1713
StyI	CCWWGG	2 cut(s)	895, 1645
TaaI	ACNGT	8 cut(s)	596, 853, 923, 960, 1591, 1905, 2227, 2245
TaiI	ACGT	1 cut(s)	1966
TaqI	TCGA	8 cut(s)	433, 530, 609, 1242, 1510, 1519, 1774, 2094
TatI	WGTACW	3 cut(s)	255, 1836, 2047
TfiI	GAWTC	2 cut(s)	599, 1073
Tru1I	TTAA	8 cut(s)	56, 188, 294, 792, 1017, 1089, 1466, 1742
Tru9I	TTAA	8 cut(s)	56, 188, 294, 792, 1017, 1089, 1466, 1742
TscAI	CASTG	7 cut(s)	172, 928, 963, 1066, 1231, 1377, 2033
TseFI	GTSAC	2 cut(s)	1189, 2271
TseI	GCWGC	5 cut(s)	749, 764, 1160, 1342, 1868
Tsp45I	GTSAC	2 cut(s)	1189, 2271
TspDTI	ATGAA	6 cut(s)	1277, 1623, 1643, 1989, 2078, 2333
TspRI	CASTG	7 cut(s)	172, 928, 963, 1066, 1231, 1377, 2033
VpaK11BI	GGWCC	4 cut(s)	448, 631, 1526, 1681
XapI	RAATTY	6 cut(s)	392, 890, 1092, 1353, 1946, 2320
XhoI	CTCGAG	1 cut(s)	2093
XmnI	GAANNNNTTC	1 cut(s)	64
XspI	CTAG	4 cut(s)	315, 915, 1283, 2073

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.