Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0000463

Physical Location & Seq

Genomic Coordinates Reverse (-)

5706999 .. 5710148

Gene Structure 3150 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G006000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3150 bp

ATGGGGTGTGAGAGCAACCTGAAGACATGGGTGTCTGATAAGTTAATAGCATTGCTCGGATACTCTAATCCGGTAGTTGTTCAGTATGTTATTGGATTAACTAAAAAAGCAAACTCCCAGGCTGGTTTGGTGGACAAGCTTGTCGAATTTGGATTTGCGTCATCGACTGAAACAGGGGCATTTGCTGCAGATATCTTTGCAAGAGTGTGTGGTGAAACATCTGGTTTGAAGCAATGTGGGAAACAAGAGAGGGGAGGTGCAATGCCGGTGAGGATTATGCAGCGGACCTATGTTCTGTTGGATTCAGATGATGATGGTGATGCAGAGAGATGTTCTGTTGAGGTTGTTTCTCATTCTGTGTCTGTAACAGTGGATTTCAATAAGAAAAGATTCAGGAAGAGGGCATTGGGTCAGAAAGATGGAGATGATGATGAGGTTATTGCAGAACGTGTTAAAAGGCGGAACTTGCCAGATAAAGATGATCATGAGGATGGTTCAGAGTCAGAAGAAGAGAGATTGCGTGATCAAAGAGAAAGGGAGAAATTGGAGCAAAGTATATGGAAGAGGGACGCAGCAGCCACACGAAAACTGACAATGAAAAACTTAAGACGAAAAGGGGAAGAGGAGGCTGTTCGAAAAACCAGTGGTGATGTTGAAGGTTTAAGAAGAGCTTCAAGACAAGAATATCTAAAGGAAAGACAGAGAAAGAAAATGGATGAAATGAGGGATGATGTAGAAGATGAACGTTACTTATTTGAAGGTGTGAAGCTCACTGAAGCAGAAAACCGGGAATTAAGTTACAAGAAGCAAATATTGGAGCTTATGAGAAAACCATTAGGTCTAGATGAGGCTGAAAATGTCACTGAGTACAGGATGCCAGATGCCTATGATGATGAGGAGCGCGGTGTTACTCAACAGAAGAGATTTTCTGTGGCTTTGCAGCGCTACAAGGACGACAGTTCTGGGGATAAAATGAACTCGTTTGGACAACATAAATCTTGGGAAGATCAGCAACTTGGAAAAGCAACATTGGAATTTGGTTCAAAAAACAAAAAAAGGGTATCTGATGAGTATGATTTTGTATATGAAGACCAGATTGAGTTCGTCAGGGGGTTGGTGATCGAGGATGATAAATATGACAAGTTTGAGGATGATAAGCAACTGCAGTCCACAAAATTACTTGAGTCAAGGGAAAAAACCTTGAAGATGTTCCAGGAGGAGAGGAAAACTTTACCCATCTTCTCATTTCATGATGAGTTGCTTCAAGCTGTTAAAGATCATCAGGTTCTTGTTATTGTTGGCGAAACTGGATCTGGCAAAACTACACAGATTCCCCAGTATTTACATGAGGCAGGATATACAAGGCATGGAAAGATTGGGTGCACACAGCCACGGCGAGTTGCTGCTATGAGTGTTGCTGCCAGGGTTTCTCAAGAAATGGGTGTCAAGCTTGGACATGAGGTAGGTTATTCTATTCGTTTTGAGGATTGCACGTCTGAAAAGACCGTTTTGAAATATATGACTGATGGAATGTTATTGCGTGAATTTCTTGCTGAACCAGATTTGGCAAGCTATAGTGTGCTGATGGTGGATGAGGCCCATGAGAGAACACTCTCTACTGATATTCTATTTGCATTAGTAAAGGATATTGCTCGATTTCGACCCGGTTTTAAGCTGCTTATCTCGAGTGCAACACTTGATGCTGTGAAGTTCAGTAATTATTTTGATTGTGCCCCAATTTTCAACATTCCAGGGAGGCGGTTTCCTGTCGATATATACCACACAAAAGCACCAGAAGCTGATTACTTAGATGCTGCAATTGCTGCTGCAATTCAAATCCATGTCAGGGAACCACCTGGAGACATATTGGTCTTCCTCACGGGTCAAGAAGATATAGAAACAGCAAGCGAAACATTAAATTACAGGACAAGTGGTCTCGGGAGAAAGATATCAGAGCTGATTATCTGTCCCATATATGCAAACCTGCCTACTGAGCAGCAAGCAAAAATTTTTGAGCCCACACCTGAAGGGGCTAGAAAGGTTGTCCTTGCCACAAACATAGCTGAAACTTCTCTGACTATTGATGGGATCAAATATGTCATCGACTGTGGCTATTGCAAGATGATGTCCTATAATCCAAGGACTGGGATGGAGTCATTGCAAGTCACTCCCATCTCAAAGGCATCAGCAAGGCAGAGGGCAGGTCGATCTGGTCGAACAGGCCCTGGTAAGTGTTTTCGGTTATATACTGTTGACAGTTATATTCATGAATTAGATGATACCACAATACCTGAAATACTAAGGGCTAACCTGGCAAATGTTGTTCTTAGGCTTAAGAGCCTTGGTATCCATGACTTGATACATTTTGATTTTATGGATCCTCCACCATCGGAAGCATTACTAAAAGCCCTCGAACTGTTATTTGCTTTATCTGCATTGAATAAAATGGGAGAGTTGACTAAAGTTGGTAGGCGGATGGCAGAGTTTCCACTTGATCCAATGCTATCTAAGATGGTAGTTGCTTCTGACAGGTACAATTGCTCAAACGAGATCATTTCTATTGCTGCCATGCTTTCTGTCGGTAATTCAATCTTTTATCGTCCAAAGGACAAACAAGTCTATGCTGACAATGCAAGACGGACTTTTCACACTGGGGACACAGGAGATCAGATTGCCTTGCTTAATGTTTACAATACATGGACCGAAACAAACTACTCAAGTCAATGGTGTTATGAAAACTATATAAATGCTAGGAGCATGAAAAGAGCAAGAGATATCAGGGATCAACTTAAGAGACTCTTGGAGAGGGTCGAGATCGAGCTAACCTCAAATCTTGACGATTTAGAGTCTATAAAGAAGGCCATTACATCAGGTTTCTTCCCTCATTGTGCAAGGCTGCGAAAGAATGGATCTTATAAAATAGTCAAACATCAACAGGCTGCCTCCATACACCCCAGCTCAGGCTTGTCACAGGAGTTTCCGACATGGGTTTTATACCATGAACTGGTGCTTACATCCAAGGAATACATGAGACAGGTAACAGAGTTGAAGCCCGAGTGGTTGCTGGAAATAGCTCCACATTATTACCAACTAGAGGATGTCGATGCTGCTTTGACCTTCAAGAAAACGTCTAGTGGCAATGTAGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009790 GO:0009791 GO:0009793 GO:0009892 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0016020 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031047 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1049

Amino Acids

119.32

Weight (kDa)

6.0

Isoelectric Point (pI)

40.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_22	PF13401	426 - 553	1.8e-06	AAA domain
Helicase_C	PF00271	610 - 741	6.4e-13	Helicase conserved C-terminal domain
WHD_HA2	PF04408	803 - 831	7.2e-10	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	832 - 893	3.8e-10	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	951 - 1028	3.2e-24	Oligonucleotide/oligosaccharide-binding (OB)-fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000463)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32490 AT1G32490 AT2G35340 AT2G35340 AT4G16680 AT4G16680
fragaria_vesca	FvH4_1g06760 FvH4_1g06761 FvH4_1g06770 FvH4_3g40380 FvH4_3g40450 FvH4_5g14240 FvH4_5g14240 FvH4_5g14240 FvH4_5g14240 FvH4_7g01330 FvH4_7g01330
malus_domestica	MD02G1072400.v1.1 MD02G1072500.v1.1 MD02G1088100.v1.1 MD06G1200100.v1.1 MD07G1285300.v1.1 MD08G1042900.v1.1
prunus_persica	Prupe.5G201700_v2.0.a1 Prupe.5G201700_v2.0.a1 Prupe.6G014300_v2.0.a1 Prupe.6G159800_v2.0.a1 Prupe.7G201500_v2.0.a1 Prupe.8G103900_v2.0.a1 Prupe.8G104500_v2.0.a1
pyrus_communis	pycom02g05640 pycom02g05650 pycom14g17310
rosa_chinensis	RchiOBHm_Chr2g0092491 RchiOBHm_Chr2g0092541 RchiOBHm_Chr5g0073091 RchiOBHm_Chr6g0263651 RchiOBHm_Chr6g0263711 RchiOBHm_Chr7g0182681
rosa_laevigata	RLG00000005109 RLG00000014320 RLG00000014322 RLG00000014324 RLG00000014326 RLG00000014327 RLG00000016318 RLG00000016322 RLG00000036362
rosa_multiflora	Rmu_sc0000014.1_g000045 Rmu_sc0002174.1_g000001 Rmu_sc0003570.1_g000001 Rmu_sc0004296.1_g000005 Rmu_sc0004296.1_g000014 Rmu_sc0005592.1_g000025
rosa_roxburghii	Rroxscaffold_176G00431160 Rroxscaffold_176G00431170 Rroxscaffold_2G00148990 Rroxscaffold_2G00149040 Rroxscaffold_3G00271070 Rroxscaffold_6G00409880 Rroxscaffold_7G00204020 Rroxscaffold_7G00204040 Rroxscaffold_7G00204070
rosa_rugosa	Rorug02G0027100 Rorug02G0027200 Rorug02G0027300 Rorug05G0421000 Rorug06G0449100
rosa_samantha	Rh2AG072900 Rh2AG073200 Rh2BG073500 Rh2BG074100 Rh2CG074700 Rh2DG071600 Rh2DG072300 Rh5AG478000 Rh5BG498700 Rh5DG510800 Rh6AG125300 Rh6BG121400 Rh6CG119800 Rh7AG052900 Rh7BG052500 Rh7CG054000 Rh7DG052300
rosa_wichuraiana	Rw0G005280 Rw2G005970 Rw2G006000 Rw5G044390 Rw6G010890 Rw7G004310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2916
AasI	GACNNNNNNGTC	4 cut(s)	31, 140, 1868, 2615
Acc36I	ACCTGC	2 cut(s)	1992, 2192
AccB7I	CCANNNNNTGG	2 cut(s)	2144, 3004
AccII	CGCG	1 cut(s)	903
AciI	CCGC	5 cut(s)	283, 460, 903, 1759, 2473
AclI	AACGTT	1 cut(s)	745
AclWI	GGATC	7 cut(s)	1318, 2096, 2372, 2385, 2489, 2790, 2917
AcsI	RAATTY	4 cut(s)	146, 1034, 1544, 2007
AcuI	CTGAAG	3 cut(s)	41, 795, 2046
AfaI	GTAC	2 cut(s)	869, 2534
AfeI	AGCGCT	1 cut(s)	944
AfiI	CCNNNNNNNGG	5 cut(s)	1846, 2144, 2960, 3004, 3094
AflII	CTTAAG	3 cut(s)	604, 2333, 2789
AflIII	ACRYGT	1 cut(s)	448
AhdI	GACNNNNNGTC	1 cut(s)	1932
AjiI	CACGTC	1 cut(s)	1494
AjnI	CCWGG	7 cut(s)	117, 1212, 1421, 1750, 1855, 2224, 2310
AjuI	GAANNNNNNNTTGG	4 cut(s)	389, 421, 797, 829
Alw21I	GWGCWC	1 cut(s)	1385
Alw26I	GTCTC	4 cut(s)	1854, 1940, 2788, 3025
Alw44I	GTGCAC	1 cut(s)	1381
AlwI	GGATC	7 cut(s)	1318, 2096, 2372, 2385, 2489, 2790, 2917
AlwNI	CAGNNNCTG	2 cut(s)	1799, 2225
Ama87I	CYCGRG	3 cut(s)	1684, 1937, 3053
Aor51HI	AGCGCT	1 cut(s)	944
AoxI	GGCC	3 cut(s)	1596, 2221, 2859
ApaLI	GTGCAC	1 cut(s)	1381
ApoI	RAATTY	4 cut(s)	146, 1034, 1544, 2007
Asp700I	GAANNNNTTC	3 cut(s)	389, 670, 1208
AspLEI	GCGC	2 cut(s)	903, 945
AspS9I	GGNCC	4 cut(s)	285, 1597, 2222, 2700
AsuC2I	CCSGG	2 cut(s)	788, 1665
AsuHPI	GGTGA	5 cut(s)	224, 280, 329, 659, 1129
AsuII	TTCGAA	1 cut(s)	634
AvaI	CYCGRG	3 cut(s)	1684, 1937, 3053
AvaII	GGWCC	2 cut(s)	285, 2700
BaeGI	GKGCMC	2 cut(s)	1385, 1735
BamHI	GGATCC	1 cut(s)	2377
BanII	GRGCYC	1 cut(s)	2019
BbsI	GAAGAC	3 cut(s)	29, 1095, 1864
Bbv12I	GWGCWC	1 cut(s)	1385
BceAI	ACGGC	1 cut(s)	1409
BciT130I	CCWGG	7 cut(s)	119, 1214, 1423, 1752, 1857, 2226, 2312
BciVI	GTATCC	2 cut(s)	53, 2357
BclI	TGATCA	2 cut(s)	481, 523
BcnI	CCSGG	2 cut(s)	788, 1665
BcoDI	GTCTC	4 cut(s)	1854, 1940, 2788, 3025
BfaI	CTAG	5 cut(s)	842, 2034, 2751, 3092, 3132
BfmI	CTRYAG	3 cut(s)	186, 1163, 1573
BfoI	RGCGCY	1 cut(s)	946
BfrI	CTTAAG	3 cut(s)	604, 2333, 2789
BfuAI	ACCTGC	2 cut(s)	1992, 2192
BfuI	GTATCC	2 cut(s)	53, 2357
Bme1390I	CCNGG	9 cut(s)	119, 788, 1214, 1423, 1665, 1752, 1857, 2226, 2312
Bme18I	GGWCC	2 cut(s)	285, 2700
BmeRI	GACNNNNNGTC	1 cut(s)	1932
BmeT110I	CYCGRG	3 cut(s)	1684, 1937, 3053
BmgBI	CACGTC	1 cut(s)	1494
BmgT120I	GGNCC	4 cut(s)	285, 1597, 2222, 2700
BmiI	GGNNCC	2 cut(s)	1851, 2379
BmrFI	CCNGG	9 cut(s)	119, 788, 1214, 1423, 1665, 1752, 1857, 2226, 2312
BmrI	ACTGGG	3 cut(s)	1330, 2154, 2661
BmsI	GCATC	7 cut(s)	310, 864, 871, 1690, 1801, 2192, 3094
BmuI	ACTGGG	3 cut(s)	1330, 2154, 2661
BpiI	GAAGAC	3 cut(s)	29, 1095, 1864
BplI	GAGNNNNNCTC	2 cut(s)	2810, 2842
BpmI	CTGGAG	1 cut(s)	1878
Bpu10I	CCTNAGC	1 cut(s)	2959
Bpu14I	TTCGAA	1 cut(s)	634
BpuEI	CTTGAG	3 cut(s)	1202, 1416, 2701
BpuMI	CCSGG	2 cut(s)	788, 1665
BsaI	GGTCTC	1 cut(s)	1940
BsaJI	CCNNGG	8 cut(s)	117, 1391, 1422, 1751, 2138, 2224, 2341, 3018
BsaWI	WCCGGW	1 cut(s)	70
Bsc4I	CCNNNNNNNGG	5 cut(s)	1846, 2144, 2960, 3004, 3094
Bse118I	RCCGGY	1 cut(s)	265
Bse1I	ACTGG	6 cut(s)	642, 1312, 1336, 2149, 2656, 3009
Bse3DI	GCAATG	5 cut(s)	50, 239, 267, 2156, 3145
BseBI	CCWGG	7 cut(s)	119, 1214, 1423, 1752, 1857, 2226, 2312
BseDI	CCNNGG	8 cut(s)	117, 1391, 1422, 1751, 2138, 2224, 2341, 3018
BseLI	CCNNNNNNNGG	5 cut(s)	1846, 2144, 2960, 3004, 3094
BseMI	GCAATG	5 cut(s)	50, 239, 267, 2156, 3145
BseMII	CTCAG	3 cut(s)	855, 1983, 2973
BseNI	ACTGG	6 cut(s)	642, 1312, 1336, 2149, 2656, 3009
BseRI	GAGGAG	3 cut(s)	638, 911, 1232
BseSI	GKGCMC	2 cut(s)	1385, 1735
BseYI	CCCAGC	1 cut(s)	2954
Bsh1236I	CGCG	1 cut(s)	903
BshFI	GGCC	3 cut(s)	1598, 2223, 2861
BsiHKAI	GWGCWC	1 cut(s)	1385
BsiHKCI	CYCGRG	3 cut(s)	1684, 1937, 3053
BsiSI	CCGG	4 cut(s)	71, 266, 787, 1665
BslFI	GGGAC	3 cut(s)	581, 1953, 2669
BslI	CCNNNNNNNGG	5 cut(s)	1846, 2144, 2960, 3004, 3094
BsmAI	GTCTC	4 cut(s)	1854, 1940, 2788, 3025
BsmFI	GGGAC	3 cut(s)	581, 1953, 2669
BsnI	GGCC	3 cut(s)	1598, 2223, 2861
Bso31I	GGTCTC	1 cut(s)	1940
BsoBI	CYCGRG	3 cut(s)	1684, 1937, 3053
Bsp119I	TTCGAA	1 cut(s)	634
Bsp1286I	GDGCHC	3 cut(s)	1385, 1735, 2019
BspACI	CCGC	5 cut(s)	283, 460, 903, 1759, 2473
BspANI	GGCC	3 cut(s)	1598, 2223, 2861
BspCNI	CTCAG	3 cut(s)	856, 1984, 2972
BspFNI	CGCG	1 cut(s)	903
BspHI	TCATGA	3 cut(s)	484, 1249, 2266
BspLI	GGNNCC	2 cut(s)	1851, 2379
BspMAI	CTGCAG	2 cut(s)	190, 1167
BspMI	ACCTGC	2 cut(s)	1992, 2192
BspPI	GGATC	7 cut(s)	1318, 2096, 2372, 2385, 2489, 2790, 2917
BspQI	GCTCTTC	1 cut(s)	661
BspT104I	TTCGAA	1 cut(s)	634
BspTI	CTTAAG	3 cut(s)	604, 2333, 2789
BspTNI	GGTCTC	1 cut(s)	1940
BsrDI	GCAATG	5 cut(s)	50, 239, 267, 2156, 3145
BsrFI	RCCGGY	1 cut(s)	265
BsrI	ACTGG	6 cut(s)	642, 1312, 1336, 2149, 2656, 3009
BssAI	RCCGGY	1 cut(s)	265
BssECI	CCNNGG	8 cut(s)	117, 1391, 1422, 1751, 2138, 2224, 2341, 3018
BssT1I	CCWWGG	3 cut(s)	2138, 2341, 3018
Bst2UI	CCWGG	7 cut(s)	119, 1214, 1423, 1752, 1857, 2226, 2312
Bst4CI	ACNGT	7 cut(s)	370, 959, 1507, 2108, 2251, 2258, 2418
Bst6I	CTCTTC	6 cut(s)	392, 504, 557, 615, 661, 914
BstAFI	CTTAAG	3 cut(s)	604, 2333, 2789
BstAPI	GCANNNNNTGC	2 cut(s)	185, 1823
BstBI	TTCGAA	1 cut(s)	634
BstC8I	GCNNGC	3 cut(s)	1570, 1906, 2001
BstDEI	CTNAG	7 cut(s)	864, 1807, 1992, 2300, 2327, 2508, 2959
BstDSI	CCRYGG	1 cut(s)	1391
BstFNI	CGCG	1 cut(s)	903
BstH2I	RGCGCY	1 cut(s)	946
BstHHI	GCGC	2 cut(s)	903, 945
BstMAI	GTCTC	4 cut(s)	1854, 1940, 2788, 3025
BstMWI	GCNNNNNNNGC	9 cut(s)	185, 466, 1796, 1820, 1823, 1993, 2312, 2432, 2630
BstNI	CCWGG	7 cut(s)	119, 1214, 1423, 1752, 1857, 2226, 2312
BstSCI	CCNGG	9 cut(s)	117, 786, 1212, 1421, 1663, 1750, 1855, 2224, 2310
BstSFI	CTRYAG	3 cut(s)	186, 1163, 1573
BstSLI	GKGCMC	2 cut(s)	1385, 1735
BstUI	CGCG	1 cut(s)	903
BstV2I	GAAGAC	3 cut(s)	29, 1095, 1864
BstX2I	RGATCY	3 cut(s)	1310, 2377, 2909
BstYI	RGATCY	3 cut(s)	1310, 2377, 2909
BsuI	GTATCC	2 cut(s)	53, 2357
BsuRI	GGCC	3 cut(s)	1598, 2223, 2861
BtgI	CCRYGG	1 cut(s)	1391
BtrI	CACGTC	1 cut(s)	1494
BtsIMutI	CAGTG	5 cut(s)	375, 649, 771, 861, 2649
BveI	ACCTGC	2 cut(s)	1992, 2192
Cac8I	GCNNGC	3 cut(s)	1570, 1906, 2001
CaiI	CAGNNNCTG	2 cut(s)	1799, 2225
CciI	TCATGA	3 cut(s)	484, 1249, 2266
CfoI	GCGC	2 cut(s)	903, 945
Cfr10I	RCCGGY	1 cut(s)	265
Cfr13I	GGNCC	4 cut(s)	285, 1597, 2222, 2700
CseI	GACGC	2 cut(s)	147, 578
Csp6I	GTAC	2 cut(s)	868, 2533
CviQI	GTAC	2 cut(s)	868, 2533
DdeI	CTNAG	7 cut(s)	864, 1807, 1992, 2300, 2327, 2508, 2959
DrdI	GACNNNNNNGTC	4 cut(s)	31, 140, 1868, 2615
DriI	GACNNNNNGTC	1 cut(s)	1932
DseDI	GACNNNNNNGTC	4 cut(s)	31, 140, 1868, 2615
Eam1104I	CTCTTC	6 cut(s)	392, 504, 557, 615, 661, 914
Eam1105I	GACNNNNNGTC	1 cut(s)	1932
EarI	CTCTTC	6 cut(s)	392, 504, 557, 615, 661, 914
EciI	GGCGGA	2 cut(s)	475, 2488
Eco130I	CCWWGG	3 cut(s)	2138, 2341, 3018
Eco24I	GRGCYC	1 cut(s)	2019
Eco31I	GGTCTC	1 cut(s)	1940
Eco32I	GATATC	3 cut(s)	193, 1950, 2776
Eco47I	GGWCC	2 cut(s)	285, 2700
Eco47III	AGCGCT	1 cut(s)	944
Eco57I	CTGAAG	3 cut(s)	41, 795, 2046
Eco88I	CYCGRG	3 cut(s)	1684, 1937, 3053
EcoO109I	RGGNCCY	1 cut(s)	2222
EcoRII	CCWGG	7 cut(s)	117, 1212, 1421, 1750, 1855, 2224, 2310
EcoRV	GATATC	3 cut(s)	193, 1950, 2776
EcoT14I	CCWWGG	3 cut(s)	2138, 2341, 3018
EcoT38I	GRGCYC	1 cut(s)	2019
ErhI	CCWWGG	3 cut(s)	2138, 2341, 3018
FalI	AAGNNNNNCTT	6 cut(s)	655, 687, 2626, 2658, 2783, 2815
FaqI	GGGAC	3 cut(s)	581, 1953, 2669
FbaI	TGATCA	2 cut(s)	481, 523
FriOI	GRGCYC	1 cut(s)	2019
FspBI	CTAG	5 cut(s)	842, 2034, 2751, 3092, 3132
GlaI	GCGC	2 cut(s)	902, 944
GsaI	CCCAGC	1 cut(s)	2958
GsuI	CTGGAG	1 cut(s)	1878
HaeII	RGCGCY	1 cut(s)	946
HaeIII	GGCC	3 cut(s)	1598, 2223, 2861
HapII	CCGG	4 cut(s)	71, 266, 787, 1665
HgaI	GACGC	2 cut(s)	147, 578
HhaI	GCGC	2 cut(s)	903, 945
Hin6I	GCGC	2 cut(s)	901, 943
HinP1I	GCGC	2 cut(s)	901, 943
HincII	GTYRAC	2 cut(s)	2254, 2457
HindII	GTYRAC	2 cut(s)	2254, 2457
HindIII	AAGCTT	2 cut(s)	137, 1448
HinfI	GANTC	8 cut(s)	302, 390, 500, 1184, 1330, 2153, 2796, 2846
HpaII	CCGG	4 cut(s)	71, 266, 787, 1665
HphI	GGTGA	5 cut(s)	224, 280, 329, 659, 1129
Hpy166II	GTNNAC	6 cut(s)	133, 1170, 1383, 2254, 2457, 2689
Hpy8I	GTNNAC	6 cut(s)	133, 1170, 1383, 2254, 2457, 2689
HpyAV	CCTTC	5 cut(s)	650, 752, 2021, 2851, 3127
HpyCH4III	ACNGT	7 cut(s)	370, 959, 1507, 2108, 2251, 2258, 2418
HpyCH4IV	ACGT	4 cut(s)	448, 745, 1493, 3128
HpyF10VI	GCNNNNNNNGC	9 cut(s)	185, 466, 1796, 1820, 1823, 1993, 2312, 2432, 2630
HpyF3I	CTNAG	7 cut(s)	864, 1807, 1992, 2300, 2327, 2508, 2959
HpySE526I	ACGT	4 cut(s)	448, 745, 1493, 3128
HspAI	GCGC	2 cut(s)	901, 943
Ksp22I	TGATCA	2 cut(s)	481, 523
LguI	GCTCTTC	1 cut(s)	661
LmnI	GCTCC	5 cut(s)	547, 817, 898, 2754, 3079
LweI	GCATC	7 cut(s)	310, 864, 871, 1690, 1801, 2192, 3094
MaeI	CTAG	5 cut(s)	842, 2034, 2751, 3092, 3132
MaeII	ACGT	4 cut(s)	448, 745, 1493, 3128
MaeIII	GTNAC	8 cut(s)	364, 746, 797, 859, 907, 2164, 2967, 3037
MfeI	CAATTG	2 cut(s)	1818, 2536
MflI	RGATCY	3 cut(s)	1310, 2377, 2909
MhlI	GDGCHC	3 cut(s)	1385, 1735, 2019
MlyI	GAGTC	5 cut(s)	509, 1193, 2162, 2790, 2855
MmeI	TCCRAC	2 cut(s)	279, 3005
MroXI	GAANNNNTTC	3 cut(s)	389, 670, 1208
MslI	CAYNNNNRTG	1 cut(s)	489
MspA1I	CMGCKG	1 cut(s)	283
MspCI	CTTAAG	3 cut(s)	604, 2333, 2789
MspI	CCGG	4 cut(s)	71, 266, 787, 1665
MspR9I	CCNGG	9 cut(s)	119, 788, 1214, 1423, 1665, 1752, 1857, 2226, 2312
MunI	CAATTG	2 cut(s)	1818, 2536
MvaI	CCWGG	7 cut(s)	119, 1214, 1423, 1752, 1857, 2226, 2312
MvnI	CGCG	1 cut(s)	903
MwoI	GCNNNNNNNGC	9 cut(s)	185, 466, 1796, 1820, 1823, 1993, 2312, 2432, 2630
NciI	CCSGG	2 cut(s)	788, 1665
NlaIV	GGNNCC	2 cut(s)	1851, 2379
NmuCI	GTSAC	3 cut(s)	859, 2164, 2967
NspV	TTCGAA	1 cut(s)	634
PaeR7I	CTCGAG	1 cut(s)	1684
PagI	TCATGA	3 cut(s)	484, 1249, 2266
PciSI	GCTCTTC	1 cut(s)	661
PcsI	WCGNNNNNNNCGW	1 cut(s)	2212
PdmI	GAANNNNTTC	3 cut(s)	389, 670, 1208
PfeI	GAWTC	3 cut(s)	302, 390, 1330
PflMI	CCANNNNNTGG	2 cut(s)	2144, 3004
PfoI	TCCNGGA	1 cut(s)	1212
PleI	GAGTC	5 cut(s)	508, 1192, 2161, 2790, 2854
PpsI	GAGTC	5 cut(s)	508, 1192, 2161, 2790, 2854
PsiI	TTATAA	1 cut(s)	2916
Psp1406I	AACGTT	1 cut(s)	745
Psp6I	CCWGG	7 cut(s)	117, 1212, 1421, 1750, 1855, 2224, 2310
PspFI	CCCAGC	1 cut(s)	2954
PspGI	CCWGG	7 cut(s)	117, 1212, 1421, 1750, 1855, 2224, 2310
PspN4I	GGNNCC	2 cut(s)	1851, 2379
PspPI	GGNCC	4 cut(s)	285, 1597, 2222, 2700
PsrI	GAACNNNNNNTAC	2 cut(s)	1600, 1632
PstI	CTGCAG	2 cut(s)	190, 1167
PstNI	CAGNNNCTG	2 cut(s)	1799, 2225
PsuI	RGATCY	3 cut(s)	1310, 2377, 2909
RsaI	GTAC	2 cut(s)	869, 2534
RsaNI	GTAC	2 cut(s)	868, 2533
RseI	CAYNNNNRTG	1 cut(s)	489
SapI	GCTCTTC	1 cut(s)	661
Sau96I	GGNCC	4 cut(s)	285, 1597, 2222, 2700
SchI	GAGTC	5 cut(s)	509, 1193, 2162, 2790, 2855
ScrFI	CCNGG	9 cut(s)	119, 788, 1214, 1423, 1665, 1752, 1857, 2226, 2312
SduI	GDGCHC	3 cut(s)	1385, 1735, 2019
SfaNI	GCATC	7 cut(s)	310, 864, 871, 1690, 1801, 2192, 3094
SfcI	CTRYAG	3 cut(s)	186, 1163, 1573
Sfr274I	CTCGAG	1 cut(s)	1684
SfuI	TTCGAA	1 cut(s)	634
SinI	GGWCC	2 cut(s)	285, 2700
SlaI	CTCGAG	1 cut(s)	1684
SmiMI	CAYNNNNRTG	1 cut(s)	489
SmlI	CTYRAG	7 cut(s)	604, 1181, 1431, 1684, 2333, 2716, 2789
SmoI	CTYRAG	7 cut(s)	604, 1181, 1431, 1684, 2333, 2716, 2789
SsiI	CCGC	5 cut(s)	283, 460, 903, 1759, 2473
SspI	AATATT	1 cut(s)	813
SspMI	CTAG	5 cut(s)	842, 2034, 2751, 3092, 3132
StyD4I	CCNGG	9 cut(s)	117, 786, 1212, 1421, 1663, 1750, 1855, 2224, 2310
StyI	CCWWGG	3 cut(s)	2138, 2341, 3018
TaaI	ACNGT	7 cut(s)	370, 959, 1507, 2108, 2251, 2258, 2418
TaiI	ACGT	4 cut(s)	451, 748, 1496, 3131
TaqII	GACCGA	1 cut(s)	2717
TatI	WGTACW	1 cut(s)	867
TfiI	GAWTC	3 cut(s)	302, 390, 1330
TscAI	CASTG	5 cut(s)	375, 649, 778, 868, 2656
TseFI	GTSAC	3 cut(s)	859, 2164, 2967
Tsp45I	GTSAC	3 cut(s)	859, 2164, 2967
TspGWI	ACGGA	1 cut(s)	2653
TspRI	CASTG	5 cut(s)	375, 649, 778, 868, 2656
Van91I	CCANNNNNTGG	2 cut(s)	2144, 3004
Vha464I	CTTAAG	3 cut(s)	604, 2333, 2789
VneI	GTGCAC	1 cut(s)	1381
VpaK11BI	GGWCC	2 cut(s)	285, 2700
XapI	RAATTY	4 cut(s)	146, 1034, 1544, 2007
XbaI	TCTAGA	1 cut(s)	841
XhoI	CTCGAG	1 cut(s)	1684
XmnI	GAANNNNTTC	3 cut(s)	389, 670, 1208
XspI	CTAG	5 cut(s)	842, 2034, 2751, 3092, 3132

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.