Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0000463

Physical Location & Seq

Genomic Coordinates Forward (+)

8027815 .. 8037331

Gene Structure 9517 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g14240.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3129 bp

ATGGGGAGCGAGAGCAATTTGAAGACGTGGGTTTCCGATAAGCTGATGACATTGCTCGGTTATTCTCAGCCAGCTGTTGTTCAGTACATTATTGGAATAAGTAAACAAGCTAAGTCTCCGGCTGAAGTGGTGGTGAAGCTTGTAGATTCCGGGTGGACCTCCTCGTCTGATACACGTAAATTTGCTCAGGAAATATTCTCCAAAGTGCCCCACAAATCATCTGGACCGAATGATTATCAGAAACAAGAGAGGGAAGCCGCGATGTTGGTTAGGAAGCAGAAGACATATGCTCTCTTGGATGCAGATGACGACGATGATGAGGATGATAAATCTGCTGTGCCAGTAGTTTCCGAGTCCAGGAAAAGTGATTCCCATAAGAAACGGTTCAGGAAGAAGGCCTCGAGTGAAGACGACGAAGACGATGAGGTGATTGTACACCAGGAAGATGTGAGACGGGTTAAAAGACGAACTTCCCCGGATGAAGATGATGGTTCAGAGTCGGAAGAGGAAAGGTTGCGTGATCAAAGAGAGCGGGAGGAATTAGAGCGCAATTTAAGGGAGAGGGACGCCGCAAATACACGAAAGTTAACAGAGAGAAAGTTATCAAAAAAAGAGGAAGAGGAGGCTATTCGGAGAAATAAGGCTTCAGAGCGAAATGAAACTGAAGATTTAAGAGTAGTTTCAAGACAAGAGTATTTAAAGAAAAGAGAGCAGAAGAAATTGGAGGAAATGAGAGATGAGATAGAAGATGAGCAATACTTATTTGAGAACGTGGAGCTCACTGAAGCGGAACGTCGTGAATATAGTTACAAGAAGAAAATACTTGAGGCTGTGGAAAAGCGGGCAGTGGAGGATGAGAATCAAAATGAGTACAGGATTCCTGATGCCTATGATGTGGAGGGGGGTGTCAATCAGGAGAAGAGGTTTAATGTGGCCTTAACACGTTACAGGGACCTGGCTGGGGAGAAAATGAATCCATTTGCAGAACAGGAAGCTTGGGAGGATCACCAAATTGGAAAGGCAACACTGAAGTATGGTTCAAAAAACAAAAAGCGATCTGATGAGTATCAATTTGTATTTGAGGACCAGATTGATTTTATCAAGGCATCAGTAATGGATGGTGATCAGTTTGAAGATGCAGAACCCACTGATTTACTTGAGTTGAGAGCGAAATCAGAGTTGGAGAAGCTCCAGGAGGACAGAAAAACATTACCCATATACCTTTATCGTGATGAGTTGCTCAAAGCTGTTGATGACCATCAGGTTCTTGTTATCGTGGGTGAAACCGGTTCTGGAAAAACTACGCAAATACCTCAGTATCTTCATGAGGCCGGATACACAAAACGTGGAAAGATTGGGTGCACACAGCCACGACGAGTTGCTGCTATGAGTGTTGCTGCAAGGGTTTCTCAAGAAATGGGTGTGAAACTTGGGCATGAGGTAGGTTATTCCATTCGTTTTGAGGATTGCACATCAGAGAAGACGGTTTTGAAATATATGACAGATGGGATGTTGTTGCGAGAATTCCTTGGTGAACCAGATCTGGCAGGCTACAGTGTGGTTATGGTGGATGAGGCCCATGAGAGGACACTTTCAACTGATATTCTATTTGGATTAGTAAAGGATATTGCTCGATTTCGACCTGATCTTAAACTACTTATCTCGAGTGCAACGCTTGATGCTGAGAAGTTCAGTGATTATTTTGATTCTGCTCCAATTTTCAAAATTCCGGGGCGGCGTTATCCTGTTGAAATAAATTACACAAAAGCACCAGAAGCTGATTATTTGGATGCAGCAATTGTGACTGCACTGCAAATCCATGTGACAGAAGCACCTGGAGATATATTAGTGTTCCTGACAGGTCAGGAAGAAATTGAAACAGCAGAAGAAATATTGAAGCACAGAACAAGGGGCTTAGGGACAAAAATTGCGGAGCTTATTATTTGTCCTATATATGCAAACCTACCTACAGAGCTGCAAGCCAAAATTTTTGAGCCCACACCTGAAGGGGCAAGGAAAGTTGTGCTTGCCACAAATATAGCTGAAACTTCATTAACTATTGATGGGATCAAATATGTTATTGACCCAGGGTTTTGCAAGATGAAGTCATACAATCCAAGGACTGGGATGGAGTCGTTGCTGGTCGCTCCCATTTCAAAGGCATCAGCCAATCAGAGGGCAGGTCGATCTGGGAGAACGGGTCCCGGAAAGTGCTACCGGCTATATACTATGTTCAATTACCAAACTGAGTTAGAAGATAACACGGTACCCGAAATACAGAGGACTAACCTTGCAAATGTTGTGCTCATGCTGAAGAGTCTTGGTATCCATGACCTATTACATTTTGATTTTATGGACCCTCCACCATCAGAGGCATTACTGAAAGCCCTGGAACTGTTATTTGCACTAAGTGCATTGAATAAAGTGGGTGAATTGACTAAAGTTGGTAGAAGGATGGCGGAGTTTCCTCTTGATCCTATGCTATCTAAGATGATAGTTGCTTCTGACAAGTACAAGTGTTCAGACGAGATCATTTCCATTGCTTCCATGCTTTCCATTGGTAATTCAATATTTTACCGTCCAAAGGACAAGCAAGTCCATGCTGACAATGCACGGTTGAATTTTCACACTGGGAATGTGGGAGATCACATTGCATTGCTCAAGGTTTTTAATTCATGGAAAGAGACTAATTTCTCAACACAATGGTGTTACGAAAATTATATACAGGTCAGGAGTATGAAGCGCGCTAGAGATATCAGAGACCAGCTTGAGGGACTCTTAGAGAGGGTCGAGATTGAGCAGGTCTCAAATCTTGATTTTGAAGTTATAAAGAAGGCCATTACATCTGGCTTTTTCCCTCATTCTTCAAGACTGCAAAAGAGTGGAGCTTATAGAACAGTCAAACATCCACAGACTGTCCACATACATCCCAGCTCAGGGCTTTCTCAGGTGCTTCCAAGATGGGTCATATACCATGAGTTAGTGCTAACAACCAAGGAATATATGAGACAGGTAACGGAACTCAAGCCAGAGTGGTTGGTGGAAATAGCTCCGCATTATTACCAGCTAAAAGATGTTGAAGACTCTGTGACAAAGAAAATGCCTCGTGGAGAAGGACGGGCATCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009790 GO:0009791 GO:0009793 GO:0009892 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0016020 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031047 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1043

Amino Acids

119.26

Weight (kDa)

5.6

Isoelectric Point (pI)

43.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Helicase_C	PF00271	608 - 734	1.2e-11	Helicase conserved C-terminal domain
WHD_HA2	PF04408	796 - 824	9.7e-10	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	825 - 886	4.5e-11	Helicase associated domain (HA2), ratchet-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000463)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32490 AT1G32490 AT2G35340 AT2G35340 AT4G16680 AT4G16680
fragaria_vesca	FvH4_1g06760 FvH4_1g06761 FvH4_1g06770 FvH4_3g40380 FvH4_3g40450 FvH4_5g14240 FvH4_5g14240 FvH4_5g14240 FvH4_5g14240 FvH4_7g01330 FvH4_7g01330
malus_domestica	MD02G1072400.v1.1 MD02G1072500.v1.1 MD02G1088100.v1.1 MD06G1200100.v1.1 MD07G1285300.v1.1 MD08G1042900.v1.1
prunus_persica	Prupe.5G201700_v2.0.a1 Prupe.5G201700_v2.0.a1 Prupe.6G014300_v2.0.a1 Prupe.6G159800_v2.0.a1 Prupe.7G201500_v2.0.a1 Prupe.8G103900_v2.0.a1 Prupe.8G104500_v2.0.a1
pyrus_communis	pycom02g05640 pycom02g05650 pycom14g17310
rosa_chinensis	RchiOBHm_Chr2g0092491 RchiOBHm_Chr2g0092541 RchiOBHm_Chr5g0073091 RchiOBHm_Chr6g0263651 RchiOBHm_Chr6g0263711 RchiOBHm_Chr7g0182681
rosa_laevigata	RLG00000005109 RLG00000014320 RLG00000014322 RLG00000014324 RLG00000014326 RLG00000014327 RLG00000016318 RLG00000016322 RLG00000036362
rosa_multiflora	Rmu_sc0000014.1_g000045 Rmu_sc0002174.1_g000001 Rmu_sc0003570.1_g000001 Rmu_sc0004296.1_g000005 Rmu_sc0004296.1_g000014 Rmu_sc0005592.1_g000025
rosa_roxburghii	Rroxscaffold_176G00431160 Rroxscaffold_176G00431170 Rroxscaffold_2G00148990 Rroxscaffold_2G00149040 Rroxscaffold_3G00271070 Rroxscaffold_6G00409880 Rroxscaffold_7G00204020 Rroxscaffold_7G00204040 Rroxscaffold_7G00204070
rosa_rugosa	Rorug02G0027100 Rorug02G0027200 Rorug02G0027300 Rorug05G0421000 Rorug06G0449100
rosa_samantha	Rh2AG072900 Rh2AG073200 Rh2BG073500 Rh2BG074100 Rh2CG074700 Rh2DG071600 Rh2DG072300 Rh5AG478000 Rh5BG498700 Rh5DG510800 Rh6AG125300 Rh6BG121400 Rh6CG119800 Rh7AG052900 Rh7BG052500 Rh7CG054000 Rh7DG052300
rosa_wichuraiana	Rw0G005280 Rw2G005970 Rw2G006000 Rw5G044390 Rw6G010890 Rw7G004310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2828
AasI	GACNNNNNNGTC	2 cut(s)	163, 2594
Acc36I	ACCTGC	2 cut(s)	2171, 2791
Acc65I	GGTACC	1 cut(s)	2266
AccB1I	GGYRCC	1 cut(s)	2266
AccB7I	CCANNNNNTGG	1 cut(s)	2123
AccBSI	CCGCTC	1 cut(s)	532
AccII	CGCG	2 cut(s)	260, 2745
AciI	CCGC	9 cut(s)	258, 532, 570, 788, 841, 1735, 1931, 2459, 3053
AclWI	GGATC	3 cut(s)	1011, 2075, 2468
AcsI	RAATTY	5 cut(s)	179, 1523, 1725, 1986, 2620
AcuI	CTGAAG	7 cut(s)	144, 630, 684, 804, 1049, 2025, 2333
AcyI	GRCGYC	1 cut(s)	567
AdeI	CACNNNGTG	1 cut(s)	2411
AfaI	GTAC	5 cut(s)	86, 435, 872, 2268, 2513
AfiI	CCNNNNNNNGG	9 cut(s)	357, 961, 1584, 2123, 2175, 2584, 2770, 2936, 2937
AflIII	ACRYGT	2 cut(s)	173, 941
AgeI	ACCGGT	1 cut(s)	1286
AjiI	CACGTC	1 cut(s)	27
AjnI	CCWGG	7 cut(s)	356, 438, 954, 1191, 1834, 2086, 2388
AjuI	GAANNNNNNNTTGG	2 cut(s)	1163, 1195
AleI	CACNNNNGTG	1 cut(s)	2704
Alw21I	GWGCWC	3 cut(s)	780, 1364, 2307
Alw26I	GTCTC	6 cut(s)	120, 445, 2678, 2754, 2809, 3001
Alw44I	GTGCAC	1 cut(s)	1360
AlwI	GGATC	3 cut(s)	1011, 2075, 2468
AlwNI	CAGNNNCTG	1 cut(s)	1778
Ama87I	CYCGRG	2 cut(s)	400, 1663
AoxI	GGCC	5 cut(s)	396, 933, 1329, 1575, 2835
ApaLI	GTGCAC	1 cut(s)	1360
ApeKI	GCWGC	4 cut(s)	1382, 1397, 1793, 1975
ApoI	RAATTY	5 cut(s)	179, 1523, 1725, 1986, 2620
AsiGI	ACCGGT	1 cut(s)	1286
Asp700I	GAANNNNTTC	1 cut(s)	383
Asp718I	GGTACC	1 cut(s)	2266
AspLEI	GCGC	3 cut(s)	549, 2745, 2747
AspS9I	GGNCC	7 cut(s)	156, 224, 952, 1084, 1576, 2201, 2356
AsuC2I	CCSGG	4 cut(s)	151, 476, 1731, 2205
AsuHPI	GGTGA	7 cut(s)	145, 439, 998, 1133, 1292, 1544, 2441
AvaI	CYCGRG	2 cut(s)	400, 1663
AvaII	GGWCC	6 cut(s)	156, 224, 952, 1084, 2201, 2356
BaeGI	GKGCMC	2 cut(s)	210, 1364
BaeI	ACNNNNGTAYC	2 cut(s)	1301, 1334
BanI	GGYRCC	1 cut(s)	2266
BanII	GRGCYC	2 cut(s)	780, 1998
BauI	CACGAG	1 cut(s)	3105
BbsI	GAAGAC	6 cut(s)	29, 287, 414, 423, 1487, 3087
Bbv12I	GWGCWC	3 cut(s)	780, 1364, 2307
BbvI	GCAGC	4 cut(s)	1369, 1384, 1805, 1962
BccI	CCATC	9 cut(s)	482, 1112, 1266, 1499, 2057, 2122, 2374, 2449, 2955
BciT130I	CCWGG	7 cut(s)	358, 440, 956, 1193, 1836, 2088, 2390
BciVI	GTATCC	2 cut(s)	1328, 2336
BclI	TGATCA	2 cut(s)	520, 1123
BcnI	CCSGG	4 cut(s)	151, 476, 1731, 2205
BcoDI	GTCTC	6 cut(s)	120, 445, 2678, 2754, 2809, 3001
BfaI	CTAG	1 cut(s)	2748
BfmI	CTRYAG	2 cut(s)	1552, 1968
BfuAI	ACCTGC	2 cut(s)	2171, 2791
BfuI	GTATCC	2 cut(s)	1328, 2336
BglII	AGATCT	1 cut(s)	1540
BisI	GCNGC	7 cut(s)	258, 570, 1383, 1398, 1736, 1794, 1976
BlsI	GCNGC	7 cut(s)	259, 571, 1384, 1399, 1737, 1795, 1977
Bme18I	GGWCC	6 cut(s)	156, 224, 952, 1084, 2201, 2356
BmeT110I	CYCGRG	2 cut(s)	400, 1663
BmgBI	CACGTC	1 cut(s)	27
BmgT120I	GGNCC	7 cut(s)	156, 224, 952, 1084, 1576, 2201, 2356
BmiI	GGNNCC	5 cut(s)	953, 2202, 2203, 2268, 2358
BmrI	ACTGGG	2 cut(s)	2133, 2640
BmsI	GCATC	7 cut(s)	289, 874, 1115, 1126, 1669, 1780, 2171
BmuI	ACTGGG	2 cut(s)	2133, 2640
BpiI	GAAGAC	6 cut(s)	29, 287, 414, 423, 1487, 3087
BplI	GAGNNNNNCTC	2 cut(s)	2789, 2821
BpmI	CTGGAG	2 cut(s)	1175, 1857
Bpu10I	CCTNAGC	3 cut(s)	186, 1915, 2935
BpuEI	CTTGAG	6 cut(s)	845, 1178, 1395, 2645, 2789, 3008
BpuMI	CCSGG	4 cut(s)	151, 476, 1731, 2205
BsaAI	YACGTR	1 cut(s)	176
BsaBI	GATNNNNATC	4 cut(s)	858, 1065, 1122, 1257
BsaHI	GRCGYC	1 cut(s)	567
BsaI	GGTCTC	2 cut(s)	2754, 2809
BsaJI	CCNNGG	8 cut(s)	474, 1528, 1730, 2086, 2087, 2117, 2388, 2994
BsaWI	WCCGGW	1 cut(s)	1286
BsaXI	ACNNNNNCTCC	4 cut(s)	908, 938, 2185, 2215
Bsc4I	CCNNNNNNNGG	9 cut(s)	357, 961, 1584, 2123, 2175, 2584, 2770, 2936, 2937
Bse118I	RCCGGY	2 cut(s)	1286, 2217
Bse1I	ACTGG	3 cut(s)	341, 2128, 2635
Bse3DI	GCAATG	4 cut(s)	50, 2538, 2649, 2654
Bse8I	GATNNNNATC	4 cut(s)	858, 1065, 1122, 1257
BseBI	CCWGG	7 cut(s)	358, 440, 956, 1193, 1836, 2088, 2390
BseDI	CCNNGG	8 cut(s)	474, 1528, 1730, 2086, 2087, 2117, 2388, 2994
BseJI	GATNNNNATC	4 cut(s)	858, 1065, 1122, 1257
BseLI	CCNNNNNNNGG	9 cut(s)	357, 961, 1584, 2123, 2175, 2584, 2770, 2936, 2937
BseMI	GCAATG	4 cut(s)	50, 2538, 2649, 2654
BseMII	CTCAG	7 cut(s)	80, 200, 1328, 1674, 2238, 2949, 2960
BseNI	ACTGG	3 cut(s)	341, 2128, 2635
BsePI	GCGCGC	1 cut(s)	2743
BseRI	GAGGAG	2 cut(s)	151, 635
BseSI	GKGCMC	2 cut(s)	210, 1364
BseXI	GCAGC	4 cut(s)	1369, 1384, 1805, 1962
BseYI	CCCAGC	2 cut(s)	959, 2930
BsgI	GTGCAG	1 cut(s)	1791
Bsh1236I	CGCG	2 cut(s)	260, 2745
BshFI	GGCC	5 cut(s)	398, 935, 1331, 1577, 2837
BshNI	GGYRCC	1 cut(s)	2266
BshTI	ACCGGT	1 cut(s)	1286
BsiHKAI	GWGCWC	3 cut(s)	780, 1364, 2307
BsiHKCI	CYCGRG	2 cut(s)	400, 1663
BsiSI	CCGG	8 cut(s)	119, 150, 476, 1287, 1332, 1730, 2205, 2218
BslFI	GGGAC	5 cut(s)	578, 965, 1933, 2187, 2787
BslI	CCNNNNNNNGG	9 cut(s)	357, 961, 1584, 2123, 2175, 2584, 2770, 2936, 2937
BsmAI	GTCTC	6 cut(s)	120, 445, 2678, 2754, 2809, 3001
BsmBI	CGTCTC	1 cut(s)	445
BsmFI	GGGAC	5 cut(s)	578, 965, 1933, 2187, 2787
BsnI	GGCC	5 cut(s)	398, 935, 1331, 1577, 2837
Bso31I	GGTCTC	2 cut(s)	2754, 2809
BsoBI	CYCGRG	2 cut(s)	400, 1663
Bsp1286I	GDGCHC	5 cut(s)	210, 780, 1364, 1998, 2307
Bsp1407I	TGTACA	1 cut(s)	433
BspACI	CCGC	9 cut(s)	258, 532, 570, 788, 841, 1735, 1931, 2459, 3053
BspANI	GGCC	5 cut(s)	398, 935, 1331, 1577, 2837
BspCNI	CTCAG	7 cut(s)	79, 199, 1327, 1675, 2239, 2948, 2959
BspFNI	CGCG	2 cut(s)	260, 2745
BspHI	TCATGA	1 cut(s)	1324
BspLI	GGNNCC	5 cut(s)	953, 2202, 2203, 2268, 2358
BspMI	ACCTGC	2 cut(s)	2171, 2791
BspPI	GGATC	3 cut(s)	1011, 2075, 2468
BspT107I	GGYRCC	1 cut(s)	2266
BspTNI	GGTCTC	2 cut(s)	2754, 2809
BsrBI	CCGCTC	1 cut(s)	532
BsrDI	GCAATG	4 cut(s)	50, 2538, 2649, 2654
BsrFI	RCCGGY	2 cut(s)	1286, 2217
BsrGI	TGTACA	1 cut(s)	433
BsrI	ACTGG	3 cut(s)	341, 2128, 2635
BssAI	RCCGGY	2 cut(s)	1286, 2217
BssECI	CCNNGG	8 cut(s)	474, 1528, 1730, 2086, 2087, 2117, 2388, 2994
BssHII	GCGCGC	1 cut(s)	2743
BssNI	GRCGYC	1 cut(s)	567
BssSI	CACGAG	1 cut(s)	3105
BssT1I	CCWWGG	3 cut(s)	1528, 2117, 2994
Bst2BI	CACGAG	1 cut(s)	3105
Bst2UI	CCWGG	7 cut(s)	358, 440, 956, 1193, 1836, 2088, 2390
Bst4CI	ACNGT	9 cut(s)	384, 1486, 1556, 2266, 2397, 2579, 2616, 2899, 2917
Bst6I	CTCTTC	4 cut(s)	498, 612, 914, 2309
BstACI	GRCGYC	1 cut(s)	567
BstAPI	GCANNNNNTGC	1 cut(s)	2411
BstAUI	TGTACA	1 cut(s)	433
BstBAI	YACGTR	1 cut(s)	176
BstC8I	GCNNGC	6 cut(s)	72, 843, 1549, 1980, 2028, 2745
BstFNI	CGCG	2 cut(s)	260, 2745
BstHHI	GCGC	3 cut(s)	549, 2745, 2747
BstMAI	GTCTC	6 cut(s)	120, 445, 2678, 2754, 2809, 3001
BstMWI	GCNNNNNNNGC	3 cut(s)	1775, 2411, 2609
BstNI	CCWGG	7 cut(s)	358, 440, 956, 1193, 1836, 2088, 2390
BstSFI	CTRYAG	2 cut(s)	1552, 1968
BstSLI	GKGCMC	2 cut(s)	210, 1364
BstUI	CGCG	2 cut(s)	260, 2745
BstV1I	GCAGC	4 cut(s)	1369, 1384, 1805, 1962
BstV2I	GAAGAC	6 cut(s)	29, 287, 414, 423, 1487, 3087
BstX2I	RGATCY	1 cut(s)	1540
BstYI	RGATCY	1 cut(s)	1540
BsuI	GTATCC	2 cut(s)	1328, 2336
BsuRI	GGCC	5 cut(s)	398, 935, 1331, 1577, 2837
BtgZI	GCGATG	1 cut(s)	275
BtrI	CACGTC	1 cut(s)	27
BtsI	GCAGTG	2 cut(s)	852, 1808
BtsIMutI	CAGTG	8 cut(s)	780, 852, 1025, 1146, 1561, 1699, 1808, 2628
BveI	ACCTGC	2 cut(s)	2171, 2791
Cac8I	GCNNGC	6 cut(s)	72, 843, 1549, 1980, 2028, 2745
CaiI	CAGNNNCTG	1 cut(s)	1778
CciI	TCATGA	1 cut(s)	1324
CfoI	GCGC	3 cut(s)	549, 2745, 2747
Cfr10I	RCCGGY	2 cut(s)	1286, 2217
Cfr13I	GGNCC	7 cut(s)	156, 224, 952, 1084, 1576, 2201, 2356
CseI	GACGC	1 cut(s)	575
Csp6I	GTAC	5 cut(s)	85, 434, 871, 2267, 2512
CspAI	ACCGGT	1 cut(s)	1286
CviQI	GTAC	5 cut(s)	85, 434, 871, 2267, 2512
DraI	TTTAAA	1 cut(s)	699
DraIII	CACNNNGTG	1 cut(s)	2411
DrdI	GACNNNNNNGTC	2 cut(s)	163, 2594
DseDI	GACNNNNNNGTC	2 cut(s)	163, 2594
Eam1104I	CTCTTC	4 cut(s)	498, 612, 914, 2309
EarI	CTCTTC	4 cut(s)	498, 612, 914, 2309
EciI	GGCGGA	1 cut(s)	2474
Ecl136II	GAGCTC	1 cut(s)	778
Eco130I	CCWWGG	3 cut(s)	1528, 2117, 2994
Eco147I	AGGCCT	1 cut(s)	398
Eco24I	GRGCYC	2 cut(s)	780, 1998
Eco31I	GGTCTC	2 cut(s)	2754, 2809
Eco32I	GATATC	1 cut(s)	2755
Eco47I	GGWCC	6 cut(s)	156, 224, 952, 1084, 2201, 2356
Eco53kI	GAGCTC	1 cut(s)	778
Eco57I	CTGAAG	7 cut(s)	144, 630, 684, 804, 1049, 2025, 2333
Eco88I	CYCGRG	2 cut(s)	400, 1663
EcoICRI	GAGCTC	1 cut(s)	778
EcoO109I	RGGNCCY	2 cut(s)	952, 2201
EcoRI	GAATTC	1 cut(s)	1523
EcoRII	CCWGG	7 cut(s)	356, 438, 954, 1191, 1834, 2086, 2388
EcoRV	GATATC	1 cut(s)	2755
EcoT14I	CCWWGG	3 cut(s)	1528, 2117, 2994
EcoT38I	GRGCYC	2 cut(s)	780, 1998
ErhI	CCWWGG	3 cut(s)	1528, 2117, 2994
Esp3I	CGTCTC	1 cut(s)	445
FalI	AAGNNNNNCTT	4 cut(s)	454, 486, 2010, 2042
FaqI	GGGAC	5 cut(s)	578, 965, 1933, 2187, 2787
FauI	CCCGC	2 cut(s)	525, 834
FauNDI	CATATG	1 cut(s)	286
FbaI	TGATCA	2 cut(s)	520, 1123
Fnu4HI	GCNGC	7 cut(s)	258, 570, 1383, 1398, 1736, 1794, 1976
FriOI	GRGCYC	2 cut(s)	780, 1998
Fsp4HI	GCNGC	7 cut(s)	258, 570, 1383, 1398, 1736, 1794, 1976
FspBI	CTAG	1 cut(s)	2748
GlaI	GCGC	3 cut(s)	548, 2744, 2746
GluI	GCNGC	7 cut(s)	258, 570, 1383, 1398, 1736, 1794, 1976
GsaI	CCCAGC	2 cut(s)	963, 2934
GsuI	CTGGAG	2 cut(s)	1175, 1857
HaeIII	GGCC	5 cut(s)	398, 935, 1331, 1577, 2837
HapII	CCGG	8 cut(s)	119, 150, 476, 1287, 1332, 1730, 2205, 2218
HgaI	GACGC	1 cut(s)	575
HhaI	GCGC	3 cut(s)	549, 2745, 2747
Hin1I	GRCGYC	1 cut(s)	567
Hin6I	GCGC	3 cut(s)	547, 2743, 2745
HinP1I	GCGC	3 cut(s)	547, 2743, 2745
HincII	GTYRAC	1 cut(s)	588
HindII	GTYRAC	1 cut(s)	588
HindIII	AAGCTT	2 cut(s)	137, 993
HpaI	GTTAAC	1 cut(s)	588
HpaII	CCGG	8 cut(s)	119, 150, 476, 1287, 1332, 1730, 2205, 2218
HphI	GGTGA	7 cut(s)	145, 439, 998, 1133, 1292, 1544, 2441
Hpy166II	GTNNAC	7 cut(s)	104, 156, 436, 588, 1362, 1535, 2920
Hpy8I	GTNNAC	7 cut(s)	104, 156, 436, 588, 1362, 1535, 2920
Hpy99I	CGWCG	4 cut(s)	314, 416, 798, 1377
HpyAV	CCTTC	5 cut(s)	388, 2000, 2445, 2827, 3107
HpyCH4III	ACNGT	9 cut(s)	384, 1486, 1556, 2266, 2397, 2579, 2616, 2899, 2917
HpyCH4IV	ACGT	6 cut(s)	26, 175, 771, 793, 943, 1345
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1775, 2411, 2609
HpySE526I	ACGT	6 cut(s)	26, 175, 771, 793, 943, 1345
Hsp92I	GRCGYC	1 cut(s)	567
HspAI	GCGC	3 cut(s)	547, 2743, 2745
KflI	GGGWCCC	1 cut(s)	2201
KpnI	GGTACC	1 cut(s)	2270
Ksp22I	TGATCA	2 cut(s)	520, 1123
KspAI	GTTAAC	1 cut(s)	588
LmnI	GCTCC	8 cut(s)	6, 775, 1194, 1717, 1933, 2152, 2885, 3055
Lsp1109I	GCAGC	4 cut(s)	1369, 1384, 1805, 1962
LweI	GCATC	7 cut(s)	289, 874, 1115, 1126, 1669, 1780, 2171
MaeI	CTAG	1 cut(s)	2748
MaeII	ACGT	6 cut(s)	26, 175, 771, 793, 943, 1345
MaeIII	GTNAC	7 cut(s)	806, 944, 1801, 1822, 2708, 3013, 3088
MbiI	CCGCTC	1 cut(s)	532
MfeI	CAATTG	1 cut(s)	1797
MflI	RGATCY	1 cut(s)	1540
MhlI	GDGCHC	5 cut(s)	210, 780, 1364, 1998, 2307
MlyI	GAGTC	6 cut(s)	362, 506, 2141, 2326, 2769, 3077
MmeI	TCCRAC	2 cut(s)	480, 1161
MroXI	GAANNNNTTC	1 cut(s)	383
MslI	CAYNNNNRTG	1 cut(s)	2704
MspA1I	CMGCKG	1 cut(s)	74
MspI	CCGG	8 cut(s)	119, 150, 476, 1287, 1332, 1730, 2205, 2218
MunI	CAATTG	1 cut(s)	1797
MvaI	CCWGG	7 cut(s)	358, 440, 956, 1193, 1836, 2088, 2390
MvnI	CGCG	2 cut(s)	260, 2745
MwoI	GCNNNNNNNGC	3 cut(s)	1775, 2411, 2609
NciI	CCSGG	4 cut(s)	151, 476, 1731, 2205
NdeI	CATATG	1 cut(s)	286
NlaIV	GGNNCC	5 cut(s)	953, 2202, 2203, 2268, 2358
NmuCI	GTSAC	3 cut(s)	1801, 1822, 3088
OliI	CACNNNNGTG	1 cut(s)	2704
PaeR7I	CTCGAG	2 cut(s)	400, 1663
PagI	TCATGA	1 cut(s)	1324
PasI	CCCWGGG	1 cut(s)	2087
PauI	GCGCGC	1 cut(s)	2743
PceI	AGGCCT	1 cut(s)	398
PcsI	WCGNNNNNNNCGW	1 cut(s)	417
PdmI	GAANNNNTTC	1 cut(s)	383
PfeI	GAWTC	6 cut(s)	146, 368, 859, 877, 973, 1706
PflMI	CCANNNNNTGG	1 cut(s)	2123
PfoI	TCCNGGA	3 cut(s)	356, 1191, 2203
PinAI	ACCGGT	1 cut(s)	1286
PkrI	GCNGC	7 cut(s)	259, 571, 1384, 1399, 1737, 1795, 1977
PleI	GAGTC	6 cut(s)	361, 505, 2140, 2325, 2769, 3077
PpsI	GAGTC	6 cut(s)	361, 505, 2140, 2325, 2769, 3077
Ppu21I	YACGTR	1 cut(s)	176
PpuMI	RGGWCCY	2 cut(s)	952, 2201
PsiI	TTATAA	1 cut(s)	2828
Psp124BI	GAGCTC	1 cut(s)	780
Psp5II	RGGWCCY	2 cut(s)	952, 2201
Psp6I	CCWGG	7 cut(s)	356, 438, 954, 1191, 1834, 2086, 2388
PspFI	CCCAGC	2 cut(s)	959, 2930
PspGI	CCWGG	7 cut(s)	356, 438, 954, 1191, 1834, 2086, 2388
PspN4I	GGNNCC	5 cut(s)	953, 2202, 2203, 2268, 2358
PspPI	GGNCC	7 cut(s)	156, 224, 952, 1084, 1576, 2201, 2356
PspPPI	RGGWCCY	2 cut(s)	952, 2201
PspXI	VCTCGAGB	1 cut(s)	400
PstNI	CAGNNNCTG	1 cut(s)	1778
PsuI	RGATCY	1 cut(s)	1540
PteI	GCGCGC	1 cut(s)	2743
PvuII	CAGCTG	1 cut(s)	74
RsaI	GTAC	5 cut(s)	86, 435, 872, 2268, 2513
RsaNI	GTAC	5 cut(s)	85, 434, 871, 2267, 2512
RseI	CAYNNNNRTG	1 cut(s)	2704
SacI	GAGCTC	1 cut(s)	780
SatI	GCNGC	7 cut(s)	258, 570, 1383, 1398, 1736, 1794, 1976
Sau96I	GGNCC	7 cut(s)	156, 224, 952, 1084, 1576, 2201, 2356
SchI	GAGTC	6 cut(s)	362, 506, 2141, 2326, 2769, 3077
SduI	GDGCHC	5 cut(s)	210, 780, 1364, 1998, 2307
SfaNI	GCATC	7 cut(s)	289, 874, 1115, 1126, 1669, 1780, 2171
SfcI	CTRYAG	2 cut(s)	1552, 1968
Sfr274I	CTCGAG	2 cut(s)	400, 1663
SinI	GGWCC	6 cut(s)	156, 224, 952, 1084, 2201, 2356
SlaI	CTCGAG	2 cut(s)	400, 1663
SmiMI	CAYNNNNRTG	1 cut(s)	2704
SmlI	CTYRAG	8 cut(s)	400, 824, 1157, 1410, 1663, 2660, 2768, 3023
SmoI	CTYRAG	8 cut(s)	400, 824, 1157, 1410, 1663, 2660, 2768, 3023
SseBI	AGGCCT	1 cut(s)	398
SsiI	CCGC	9 cut(s)	258, 532, 570, 788, 841, 1735, 1931, 2459, 3053
SspI	AATATT	3 cut(s)	195, 1893, 2571
SspMI	CTAG	1 cut(s)	2748
SstI	GAGCTC	1 cut(s)	780
StuI	AGGCCT	1 cut(s)	398
StyI	CCWWGG	3 cut(s)	1528, 2117, 2994
TaaI	ACNGT	9 cut(s)	384, 1486, 1556, 2266, 2397, 2579, 2616, 2899, 2917
TaiI	ACGT	6 cut(s)	29, 178, 774, 796, 946, 1348
TaqI	TCGA	6 cut(s)	401, 1633, 1639, 1664, 2185, 2790
TaqII	GACCGA	1 cut(s)	241
TatI	WGTACW	4 cut(s)	84, 433, 870, 2511
TauI	GCSGC	3 cut(s)	260, 572, 1738
TfiI	GAWTC	6 cut(s)	146, 368, 859, 877, 973, 1706
TscAI	CASTG	8 cut(s)	787, 852, 1032, 1153, 1561, 1699, 1815, 2635
TseFI	GTSAC	3 cut(s)	1801, 1822, 3088
TseI	GCWGC	4 cut(s)	1382, 1397, 1793, 1975
Tsp45I	GTSAC	3 cut(s)	1801, 1822, 3088
TspDTI	ATGAA	8 cut(s)	495, 672, 986, 1313, 2040, 2117, 2664, 2753
TspGWI	ACGGA	1 cut(s)	3032
TspRI	CASTG	8 cut(s)	787, 852, 1032, 1153, 1561, 1699, 1815, 2635
Van91I	CCANNNNNTGG	1 cut(s)	2123
VneI	GTGCAC	1 cut(s)	1360
VpaK11BI	GGWCC	6 cut(s)	156, 224, 952, 1084, 2201, 2356
XapI	RAATTY	5 cut(s)	179, 1523, 1725, 1986, 2620
XcmI	CCANNNNNNNNNTGG	1 cut(s)	218
XhoI	CTCGAG	2 cut(s)	400, 1663
XmnI	GAANNNNTTC	1 cut(s)	383
XspI	CTAG	1 cut(s)	2748

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.