MD15G1230400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009943
Physical Location & Seq
Reverse (-)
18860546 .. 18862846
2301 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1230400.v1.1.491

Sequence Viewer

Length: 1125 bp
ATGGAGGCAAACATCTGTGATGTCAATCATTTGGACGCTGACGTCCTTCTACCTCCACGAAAGCGTCTGCTTGCTGGATTGAAGAAACAGAGTCCGGAGGGTGATAGTGCTGCATGGCTGTCTCTAGTTGCTACTTCAGTTGCAGCTGCAGATTCAGCATCAGCTTCGGCTTCTGCATCTTCTTCTTCTTCGCATTCTTCAAGTGAATTTAACACTCGCCTCAACAGTATATTGAGTTCTCATACTAATCTTTCGCCCGAGGAGCTGGTAGAGGTCTCGAATTCGGCTGCTGCTGCTGCAGCAAAGGCTGCAGAGGTTGCGAGAACTGCAGCTGAGGAAAAAGCTGCAATAGCTGCAAAGGCAGTGGCTGCAGCGAAGAGTGCTTTAGATTTGGTTGCCTCTTTTTCCGAAGAGGCAGCAGGCAAGGAAAAACACCTGAAAAAGAACAAGCTGAAGAAGCATGTTCCAGTTCAGCTCTTGTACAAAAAATACCAACCAATTGAAAATTGTAAGAAGGACGAAGAGTTGGCCCGGAAATTGCACAGAGCTATAAATAGCTCTCCGAGAATTACGAAGAATTCATCAAGTGCTGATGGCAAGGGTCATAAACACAAGAAGCCTAAAATTTCACATGGTTCCGAGAAAGTTAGGGTTCCCAATGGGGTAATTGTATTGGAACAAGGCCCAGCGTCTACTTGCAATGGACATTCTGTAGCAGATAAGGTCAACGTTGAAGGCACCATCCAAGACATGTACGAAAATGGTGGACCTGATAGAGCAGGTCGAATGGAGATGGATAATGGAGAAGCAGAATCAAGTCGTACGGAGAAAAATTGGGATGCTGCGAGTACCACCGGTAAAAAGAGGGGAAGAGTGAAGCTAAAAAAGTTGCCGTTGAGCAATTGCACCTTCAGGGATCAAGCAAATCTGAAAGAAGATACGGATGCACGAGGCTCCCCATTGATTGACATGAACATGGGTAACCCTACTGCCGCAAAGAAGCCTTTGTTCCCTGTGGAGTCTTCTGCCGATAGTATGCTGCCAATTGAAGCCACACCGGTGTGGAAATGCAAGGATTTTAAGGCTCCGGCGTGCATGAATCAAAACAAAGTCATGCAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

375

Amino Acids

39.91

Weight (kDa)

9.16

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009943)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17540 AT2G17540 AT2G17540 AT2G17540 AT4G35510
fragaria_vesca FvH4_1g10260
malus_domestica MD02G1114600.v1.1 MD15G1230400.v1.1
prunus_persica Prupe.7G183600_v2.0.a1
pyrus_communis pycom02g08950 pycom15g20540
rosa_chinensis RchiOBHm_Chr2g0097081
rosa_laevigata RLG00000016701
rosa_multiflora Rmu_co8013720.1_g000001 Rmu_sc0017953.1_g000005
rosa_roxburghii Rroxscaffold_2G00144990
rosa_rugosa Rorug02G0063500
rosa_samantha Rh2BG113000 Rh2CG115200 Rh2DG114400
rosa_wichuraiana Rw2G008640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 41
AatII GACGTC 1 cut(s) 45
Acc36I ACCTGC 1 cut(s) 770
AccB1I GGYRCC 1 cut(s) 737
AccI GTMKAC 1 cut(s) 692
AccIII TCCGGA 1 cut(s) 94
AciI CCGC 1 cut(s) 993
AclI AACGTT 1 cut(s) 729
AclWI GGATC 1 cut(s) 924
AcsI RAATTY 4 cut(s) 206, 280, 577, 624
AcuI CTGAAG 3 cut(s) 120, 473, 895
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 4 cut(s) 482, 755, 823, 850
AflIII ACRYGT 1 cut(s) 750
AgeI ACCGGT 2 cut(s) 854, 1057
AgsI TTSAA 5 cut(s) 82, 201, 503, 734, 1049
AleI CACNNNNGTG 2 cut(s) 1058, 1060
Alw26I GTCTC 2 cut(s) 126, 280
AlwI GGATC 1 cut(s) 924
AlwNI CAGNNNCTG 1 cut(s) 368
Ama87I CYCGRG 1 cut(s) 257
Aor13HI TCCGGA 1 cut(s) 94
AoxI GGCC 2 cut(s) 528, 682
ApoI RAATTY 4 cut(s) 206, 280, 577, 624
AsiGI ACCGGT 2 cut(s) 854, 1057
AspS9I GGNCC 3 cut(s) 529, 683, 767
AsuC2I CCSGG 1 cut(s) 532
AsuHPI GGTGA 1 cut(s) 113
AvaI CYCGRG 1 cut(s) 257
AvaII GGWCC 1 cut(s) 767
BanI GGYRCC 1 cut(s) 737
BauI CACGAG 1 cut(s) 948
BbsI GAAGAC 1 cut(s) 1014
BbvCI CCTCAGC 1 cut(s) 333
BccI CCATC 3 cut(s) 587, 749, 787
BceAI ACGGC 1 cut(s) 877
BcgI CGANNNNNNTGC 2 cut(s) 147, 181
BcnI CCSGG 1 cut(s) 532
BcoDI GTCTC 2 cut(s) 126, 280
BfaI CTAG 1 cut(s) 125
BfmI CTRYAG 6 cut(s) 147, 297, 309, 327, 369, 711
BfuAI ACCTGC 1 cut(s) 770
Bme1390I CCNGG 1 cut(s) 532
Bme18I GGWCC 1 cut(s) 767
BmeT110I CYCGRG 1 cut(s) 257
BmgT120I GGNCC 3 cut(s) 529, 683, 767
BmiI GGNNCC 5 cut(s) 637, 654, 739, 955, 1086
BmrFI CCNGG 1 cut(s) 532
BmsI GCATC 4 cut(s) 167, 185, 829, 934
BpiI GAAGAC 1 cut(s) 1014
Bpu10I CCTNAGC 1 cut(s) 333
BpuMI CCSGG 1 cut(s) 532
BsaBI GATNNNNATC 1 cut(s) 24
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 280
BsaJI CCNNGG 1 cut(s) 258
BsaWI WCCGGW 3 cut(s) 94, 854, 1057
Bse118I RCCGGY 2 cut(s) 854, 1057
Bse1I ACTGG 1 cut(s) 467
Bse3DI GCAATG 1 cut(s) 706
Bse8I GATNNNNATC 1 cut(s) 24
BseAI TCCGGA 1 cut(s) 94
BseDI CCNNGG 1 cut(s) 258
BseGI GGATG 3 cut(s) 741, 844, 949
BseJI GATNNNNATC 1 cut(s) 24
BseMI GCAATG 1 cut(s) 706
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 1 cut(s) 467
BseRI GAGGAG 1 cut(s) 275
BseYI CCCAGC 1 cut(s) 685
BshFI GGCC 2 cut(s) 530, 684
BshNI GGYRCC 1 cut(s) 737
BshTI ACCGGT 2 cut(s) 854, 1057
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 5 cut(s) 95, 532, 855, 1058, 1088
BsiWI CGTACG 1 cut(s) 821
BsmAI GTCTC 2 cut(s) 126, 280
BsmI GAATGC 1 cut(s) 193
BsnI GGCC 2 cut(s) 530, 684
Bso31I GGTCTC 1 cut(s) 280
BsoBI CYCGRG 1 cut(s) 257
Bsp13I TCCGGA 1 cut(s) 94
Bsp1407I TGTACA 1 cut(s) 480
Bsp143I GATC 1 cut(s) 916
BspACI CCGC 1 cut(s) 993
BspANI GGCC 2 cut(s) 530, 684
BspCNI CTCAG 1 cut(s) 325
BspEI TCCGGA 1 cut(s) 94
BspLI GGNNCC 5 cut(s) 637, 654, 739, 955, 1086
BspMAI CTGCAG 5 cut(s) 151, 301, 313, 331, 373
BspMI ACCTGC 1 cut(s) 770
BspPI GGATC 1 cut(s) 924
BspT107I GGYRCC 1 cut(s) 737
BspTNI GGTCTC 1 cut(s) 280
BsrDI GCAATG 1 cut(s) 706
BsrFI RCCGGY 2 cut(s) 854, 1057
BsrGI TGTACA 1 cut(s) 480
BsrI ACTGG 1 cut(s) 467
BssAI RCCGGY 2 cut(s) 854, 1057
BssECI CCNNGG 1 cut(s) 258
BssMI GATC 1 cut(s) 916
BssNI GRCGYC 1 cut(s) 42
BssSI CACGAG 1 cut(s) 948
Bst2BI CACGAG 1 cut(s) 948
Bst4CI ACNGT 1 cut(s) 227
Bst6I CTCTTC 4 cut(s) 371, 405, 516, 865
BstACI GRCGYC 1 cut(s) 42
BstAPI GCANNNNNTGC 4 cut(s) 308, 317, 353, 368
BstAUI TGTACA 1 cut(s) 480
BstC8I GCNNGC 3 cut(s) 72, 421, 1093
BstDEI CTNAG 1 cut(s) 333
BstEII GGTNACC 1 cut(s) 980
BstF5I GGATG 3 cut(s) 741, 844, 949
BstKTI GATC 1 cut(s) 919
BstMAI GTCTC 2 cut(s) 126, 280
BstMBI GATC 1 cut(s) 916
BstNSI RCATGY 2 cut(s) 464, 754
BstPI GGTNACC 1 cut(s) 980
BstSCI CCNGG 1 cut(s) 530
BstSFI CTRYAG 6 cut(s) 147, 297, 309, 327, 369, 711
BstV2I GAAGAC 1 cut(s) 1014
BsuRI GGCC 2 cut(s) 530, 684
BtsCI GGATG 3 cut(s) 741, 844, 949
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 1 cut(s) 369
BveI ACCTGC 1 cut(s) 770
Cac8I GCNNGC 3 cut(s) 72, 421, 1093
CaiI CAGNNNCTG 1 cut(s) 368
Cfr10I RCCGGY 2 cut(s) 854, 1057
Cfr13I GGNCC 3 cut(s) 529, 683, 767
CseI GACGC 3 cut(s) 44, 53, 678
Csp6I GTAC 4 cut(s) 481, 754, 822, 849
CspAI ACCGGT 2 cut(s) 854, 1057
CspCI CAANNNNNGTGG 2 cut(s) 345, 380
CviAII CATG 8 cut(s) 114, 461, 632, 751, 970, 976, 1096, 1114
CviQI GTAC 4 cut(s) 481, 754, 822, 849
DdeI CTNAG 1 cut(s) 333
DpnI GATC 1 cut(s) 918
DpnII GATC 1 cut(s) 916
DrdI GACNNNNNNGTC 1 cut(s) 41
DseDI GACNNNNNNGTC 1 cut(s) 41
Eam1104I CTCTTC 4 cut(s) 371, 405, 516, 865
EarI CTCTTC 4 cut(s) 371, 405, 516, 865
Eco31I GGTCTC 1 cut(s) 280
Eco47I GGWCC 1 cut(s) 767
Eco57I CTGAAG 3 cut(s) 120, 473, 895
Eco88I CYCGRG 1 cut(s) 257
Eco91I GGTNACC 1 cut(s) 980
EcoO65I GGTNACC 1 cut(s) 980
EcoRI GAATTC 2 cut(s) 280, 577
FaeI CATG 8 cut(s) 117, 464, 635, 754, 973, 979, 1099, 1117
FatI CATG 8 cut(s) 113, 460, 631, 750, 969, 975, 1095, 1113
FblI GTMKAC 1 cut(s) 692
FokI GGATG 3 cut(s) 728, 851, 956
FspBI CTAG 1 cut(s) 125
GsaI CCCAGC 1 cut(s) 689
HaeIII GGCC 2 cut(s) 530, 684
HapII CCGG 5 cut(s) 95, 532, 855, 1058, 1088
HgaI GACGC 3 cut(s) 44, 53, 678
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 8 cut(s) 117, 464, 635, 754, 973, 979, 1099, 1117
HincII GTYRAC 1 cut(s) 727
HindII GTYRAC 1 cut(s) 727
HinfI GANTC 5 cut(s) 91, 152, 812, 1019, 1099
HpaII CCGG 5 cut(s) 95, 532, 855, 1058, 1088
HphI GGTGA 1 cut(s) 113
Hpy166II GTNNAC 3 cut(s) 693, 727, 767
Hpy188I TCNGA 4 cut(s) 409, 564, 640, 930
Hpy188III TCNNGA 3 cut(s) 95, 277, 1122
Hpy8I GTNNAC 3 cut(s) 693, 727, 767
HpyAV CCTTC 4 cut(s) 56, 508, 728, 919
HpyCH4III ACNGT 1 cut(s) 227
HpyCH4IV ACGT 2 cut(s) 42, 729
HpyF3I CTNAG 1 cut(s) 333
HpySE526I ACGT 2 cut(s) 42, 729
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 8 cut(s) 117, 464, 635, 754, 973, 979, 1099, 1117
Kpn2I TCCGGA 1 cut(s) 94
Kzo9I GATC 1 cut(s) 916
LmnI GCTCC 3 cut(s) 262, 959, 1090
LweI GCATC 4 cut(s) 167, 185, 829, 934
MaeI CTAG 1 cut(s) 125
MaeII ACGT 2 cut(s) 42, 729
MaeIII GTNAC 1 cut(s) 980
MalI GATC 1 cut(s) 918
MboI GATC 1 cut(s) 916
MfeI CAATTG 3 cut(s) 498, 901, 1044
MlyI GAGTC 2 cut(s) 100, 1028
MroI TCCGGA 1 cut(s) 94
MseI TTAA 2 cut(s) 210, 1080
MslI CAYNNNNRTG 3 cut(s) 974, 1058, 1060
MspA1I CMGCKG 2 cut(s) 146, 332
MspI CCGG 5 cut(s) 95, 532, 855, 1058, 1088
MspR9I CCNGG 1 cut(s) 532
MunI CAATTG 3 cut(s) 498, 901, 1044
Mva1269I GAATGC 1 cut(s) 193
NciI CCSGG 1 cut(s) 532
NdeII GATC 1 cut(s) 916
NlaIII CATG 8 cut(s) 117, 464, 635, 754, 973, 979, 1099, 1117
NlaIV GGNNCC 5 cut(s) 637, 654, 739, 955, 1086
NspI RCATGY 2 cut(s) 464, 754
OliI CACNNNNGTG 2 cut(s) 1058, 1060
PciI ACATGT 1 cut(s) 750
PctI GAATGC 1 cut(s) 193
PfeI GAWTC 3 cut(s) 152, 812, 1099
Pfl23II CGTACG 1 cut(s) 821
PinAI ACCGGT 2 cut(s) 854, 1057
PleI GAGTC 2 cut(s) 99, 1027
PpsI GAGTC 2 cut(s) 99, 1027
PscI ACATGT 1 cut(s) 750
Psp1406I AACGTT 1 cut(s) 729
PspEI GGTNACC 1 cut(s) 980
PspFI CCCAGC 1 cut(s) 685
PspLI CGTACG 1 cut(s) 821
PspN4I GGNNCC 5 cut(s) 637, 654, 739, 955, 1086
PspPI GGNCC 3 cut(s) 529, 683, 767
PsrI GAACNNNNNNTAC 2 cut(s) 220, 252
PstI CTGCAG 5 cut(s) 151, 301, 313, 331, 373
PstNI CAGNNNCTG 1 cut(s) 368
PvuII CAGCTG 2 cut(s) 146, 332
RsaI GTAC 4 cut(s) 482, 755, 823, 850
RsaNI GTAC 4 cut(s) 481, 754, 822, 849
RseI CAYNNNNRTG 3 cut(s) 974, 1058, 1060
SaqAI TTAA 2 cut(s) 210, 1080
Sau3AI GATC 1 cut(s) 916
Sau96I GGNCC 3 cut(s) 529, 683, 767
SchI GAGTC 2 cut(s) 100, 1028
ScrFI CCNGG 1 cut(s) 532
SfaNI GCATC 4 cut(s) 167, 185, 829, 934
SfcI CTRYAG 6 cut(s) 147, 297, 309, 327, 369, 711
SgrAI CRCCGGYG 1 cut(s) 1057
SinI GGWCC 1 cut(s) 767
SmiMI CAYNNNNRTG 3 cut(s) 974, 1058, 1060
SsiI CCGC 1 cut(s) 993
SspMI CTAG 1 cut(s) 125
StyD4I CCNGG 1 cut(s) 530
TaaI ACNGT 1 cut(s) 227
TaiI ACGT 2 cut(s) 45, 732
TaqI TCGA 2 cut(s) 278, 784
TatI WGTACW 1 cut(s) 480
TauI GCSGC 1 cut(s) 995
TfiI GAWTC 3 cut(s) 152, 812, 1099
Tru1I TTAA 2 cut(s) 210, 1080
Tru9I TTAA 2 cut(s) 210, 1080
TscAI CASTG 1 cut(s) 369
TspDTI ATGAA 3 cut(s) 570, 986, 1112
TspGWI ACGGA 2 cut(s) 839, 956
TspRI CASTG 1 cut(s) 369
VpaK11BI GGWCC 1 cut(s) 767
XapI RAATTY 4 cut(s) 206, 280, 577, 624
XceI RCATGY 2 cut(s) 464, 754
XmiI GTMKAC 1 cut(s) 692
XspI CTAG 1 cut(s) 125
ZraI GACGTC 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.