pycom15g20540

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009943
Physical Location & Seq
Reverse (-)
14716167 .. 14717303
1137 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g20540.1

Sequence Viewer

Length: 1137 bp
ATGGAGGCAAACATCTGTGATGTCAATCATTTGGACGCTGATGTCCTTCTGCCTCCACGAAAGCGTCTGCTTGCTGGATTGAAGAAACAGAGTCCGGAGGGTGATAGTGCTGCATGGCTGTCTCTAGTTGCTACTTCAGCTGCAGCTGCAGATTCAGCATCAGCTTCGGCTTCTGCATCTTCTTCTTCTTCTTCTTCTCATTCTTCAAGTGAATTTAACACTCGCCTCAACAGTATATTGAGTTCTCATAACTCTAATCTTTCGCCCGAGGAGCTGGTAGAGGTCTCGAATTCGGCTGCTGCTGCTGCAGCGAAGGCTGCAGAGGTTGCGAGAGCTGCAGCTGAGGAAAAAGCTGCAATAGCTGCAAAGGCAGTGGCTGCAGCGAAGACTGCTTTAGATTTGGTTGCCTCTTTTTCCGAAGAGGCAGCAGGCAAGGAAAAACACCTGAAAAAGAACAAGCTGAAGAAGCATGTTCCAGTTCAGCTCTTGTACAAAAAATACCAACCAATTGAAAATTGTAAGAAGGACGAAGAGTTGGCCCGGAAATTGCACAGAGCTATAAATAGCTCTCCGAGAATTACGAAGAATTCATCAAGTGCTGACGGCAAGGGTCATAAACACAAGAAGCCTAAAATTTCACATGGTTCCGAGAAAGTTAGGGTTCCCAATGGGGTAATTGTATTGGAACAGAACCCAGCATATACTTGCAATGGACATTCTGTAGCAGATAAGGTCAACGTTGAAGGCACCATCCAAGACGTGTACGAAAATGGTGGACCTGATAGAGTTGGTCGAATGGAGATGGATAATGGAGAAGCAGAATCAAGTCGTACGAAGGAGAAAAATTGGGATGGTGCGAGTACCACCGGTAAAAAGAGGGGAAGAGTGAAGCTAAAAAAGTTGCCGTTGAGCAATTGCACTTTCAGGGATCAAGCAAATCTGAAAGAAGATACGGATGCAAGAGGCTCCCCATTGATTGACATGAACATGGGTAACCCTACTGCCGCAAAGAAGCCTTTGTTCCCTGTGGAGTCTTCTGCCGATAGTATGCTGCCAATTGAAGCCACACCGGTGTGGAAATGCAAGGATTTCAAGACTCCGGCATGCATGAATCAAAACAAAGTCATGCAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

379

Amino Acids

40.42

Weight (kDa)

9.21

Isoelectric Point (pI)

41.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009943)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17540 AT2G17540 AT2G17540 AT2G17540 AT4G35510
fragaria_vesca FvH4_1g10260
malus_domestica MD02G1114600.v1.1 MD15G1230400.v1.1
prunus_persica Prupe.7G183600_v2.0.a1
pyrus_communis pycom02g08950 pycom15g20540
rosa_chinensis RchiOBHm_Chr2g0097081
rosa_laevigata RLG00000016701
rosa_multiflora Rmu_co8013720.1_g000001 Rmu_sc0017953.1_g000005
rosa_roxburghii Rroxscaffold_2G00144990
rosa_rugosa Rorug02G0063500
rosa_samantha Rh2BG113000 Rh2CG115200 Rh2DG114400
rosa_wichuraiana Rw2G008640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 41
AccB1I GGYRCC 1 cut(s) 746
AccIII TCCGGA 1 cut(s) 94
AciI CCGC 1 cut(s) 1005
AclI AACGTT 1 cut(s) 738
AclWI GGATC 1 cut(s) 936
AcsI RAATTY 4 cut(s) 212, 289, 586, 633
AcuI CTGAAG 2 cut(s) 120, 482
AfaI GTAC 4 cut(s) 491, 764, 832, 862
AflIII ACRYGT 1 cut(s) 759
AgeI ACCGGT 2 cut(s) 866, 1069
AgsI TTSAA 6 cut(s) 82, 207, 512, 743, 1061, 1093
AjiI CACGTC 1 cut(s) 760
AleI CACNNNNGTG 2 cut(s) 1070, 1072
Alw26I GTCTC 2 cut(s) 126, 289
AlwI GGATC 1 cut(s) 936
AlwNI CAGNNNCTG 1 cut(s) 377
Ama87I CYCGRG 1 cut(s) 266
Aor13HI TCCGGA 1 cut(s) 94
AoxI GGCC 1 cut(s) 537
ApoI RAATTY 4 cut(s) 212, 289, 586, 633
AsiGI ACCGGT 2 cut(s) 866, 1069
AspS9I GGNCC 2 cut(s) 538, 776
AsuC2I CCSGG 1 cut(s) 541
AsuHPI GGTGA 1 cut(s) 113
AvaI CYCGRG 1 cut(s) 266
AvaII GGWCC 1 cut(s) 776
BanI GGYRCC 1 cut(s) 746
BbsI GAAGAC 2 cut(s) 392, 1026
BbvCI CCTCAGC 1 cut(s) 342
BccI CCATC 3 cut(s) 758, 796, 845
BceAI ACGGC 2 cut(s) 619, 889
BcgI CGANNNNNNTGC 2 cut(s) 147, 181
BcnI CCSGG 1 cut(s) 541
BcoDI GTCTC 2 cut(s) 126, 289
BfaI CTAG 1 cut(s) 125
BfmI CTRYAG 7 cut(s) 141, 147, 306, 318, 336, 378, 720
Bme1390I CCNGG 1 cut(s) 541
Bme18I GGWCC 1 cut(s) 776
BmeT110I CYCGRG 1 cut(s) 266
BmgBI CACGTC 1 cut(s) 760
BmgT120I GGNCC 2 cut(s) 538, 776
BmiI GGNNCC 4 cut(s) 646, 663, 748, 967
BmrFI CCNGG 1 cut(s) 541
BmsI GCATC 3 cut(s) 167, 185, 946
BpiI GAAGAC 2 cut(s) 392, 1026
Bpu10I CCTNAGC 1 cut(s) 342
BpuMI CCSGG 1 cut(s) 541
BsaBI GATNNNNATC 1 cut(s) 24
BsaI GGTCTC 1 cut(s) 289
BsaJI CCNNGG 1 cut(s) 267
BsaWI WCCGGW 3 cut(s) 94, 866, 1069
Bse118I RCCGGY 2 cut(s) 866, 1069
Bse1I ACTGG 1 cut(s) 476
Bse3DI GCAATG 1 cut(s) 715
Bse8I GATNNNNATC 1 cut(s) 24
BseAI TCCGGA 1 cut(s) 94
BseDI CCNNGG 1 cut(s) 267
BseGI GGATG 3 cut(s) 750, 856, 961
BseJI GATNNNNATC 1 cut(s) 24
BseMI GCAATG 1 cut(s) 715
BseMII CTCAG 1 cut(s) 333
BseNI ACTGG 1 cut(s) 476
BseRI GAGGAG 1 cut(s) 284
BseYI CCCAGC 1 cut(s) 694
BshFI GGCC 1 cut(s) 539
BshNI GGYRCC 1 cut(s) 746
BshTI ACCGGT 2 cut(s) 866, 1069
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 5 cut(s) 95, 541, 867, 1070, 1100
BsiWI CGTACG 1 cut(s) 830
BsmAI GTCTC 2 cut(s) 126, 289
BsnI GGCC 1 cut(s) 539
Bso31I GGTCTC 1 cut(s) 289
BsoBI CYCGRG 1 cut(s) 266
Bsp13I TCCGGA 1 cut(s) 94
Bsp1407I TGTACA 1 cut(s) 489
Bsp143I GATC 1 cut(s) 928
BspACI CCGC 1 cut(s) 1005
BspANI GGCC 1 cut(s) 539
BspCNI CTCAG 1 cut(s) 334
BspEI TCCGGA 1 cut(s) 94
BspLI GGNNCC 4 cut(s) 646, 663, 748, 967
BspMAI CTGCAG 6 cut(s) 145, 151, 310, 322, 340, 382
BspPI GGATC 1 cut(s) 936
BspT107I GGYRCC 1 cut(s) 746
BspTNI GGTCTC 1 cut(s) 289
BsrDI GCAATG 1 cut(s) 715
BsrFI RCCGGY 2 cut(s) 866, 1069
BsrGI TGTACA 1 cut(s) 489
BsrI ACTGG 1 cut(s) 476
BssAI RCCGGY 2 cut(s) 866, 1069
BssECI CCNNGG 1 cut(s) 267
BssMI GATC 1 cut(s) 928
Bst4CI ACNGT 1 cut(s) 233
Bst6I CTCTTC 3 cut(s) 414, 525, 877
BstAPI GCANNNNNTGC 3 cut(s) 326, 362, 377
BstAUI TGTACA 1 cut(s) 489
BstC8I GCNNGC 3 cut(s) 72, 430, 1105
BstDEI CTNAG 1 cut(s) 342
BstEII GGTNACC 1 cut(s) 992
BstF5I GGATG 3 cut(s) 750, 856, 961
BstKTI GATC 1 cut(s) 931
BstMAI GTCTC 2 cut(s) 126, 289
BstMBI GATC 1 cut(s) 928
BstNSI RCATGY 2 cut(s) 473, 1107
BstPI GGTNACC 1 cut(s) 992
BstSCI CCNGG 1 cut(s) 539
BstSFI CTRYAG 7 cut(s) 141, 147, 306, 318, 336, 378, 720
BstV2I GAAGAC 2 cut(s) 392, 1026
BsuRI GGCC 1 cut(s) 539
BtrI CACGTC 1 cut(s) 760
BtsCI GGATG 3 cut(s) 750, 856, 961
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 1 cut(s) 378
Cac8I GCNNGC 3 cut(s) 72, 430, 1105
CaiI CAGNNNCTG 1 cut(s) 377
Cfr10I RCCGGY 2 cut(s) 866, 1069
Cfr13I GGNCC 2 cut(s) 538, 776
CseI GACGC 2 cut(s) 44, 53
Csp6I GTAC 4 cut(s) 490, 763, 831, 861
CspAI ACCGGT 2 cut(s) 866, 1069
CspCI CAANNNNNGTGG 2 cut(s) 354, 389
CviAII CATG 8 cut(s) 114, 470, 641, 982, 988, 1104, 1108, 1126
CviQI GTAC 4 cut(s) 490, 763, 831, 861
DdeI CTNAG 1 cut(s) 342
DpnI GATC 1 cut(s) 930
DpnII GATC 1 cut(s) 928
DrdI GACNNNNNNGTC 1 cut(s) 41
DseDI GACNNNNNNGTC 1 cut(s) 41
Eam1104I CTCTTC 3 cut(s) 414, 525, 877
EarI CTCTTC 3 cut(s) 414, 525, 877
Eco31I GGTCTC 1 cut(s) 289
Eco47I GGWCC 1 cut(s) 776
Eco57I CTGAAG 2 cut(s) 120, 482
Eco88I CYCGRG 1 cut(s) 266
Eco91I GGTNACC 1 cut(s) 992
EcoO65I GGTNACC 1 cut(s) 992
EcoRI GAATTC 2 cut(s) 289, 586
EcoT22I ATGCAT 1 cut(s) 1109
FaeI CATG 8 cut(s) 117, 473, 644, 985, 991, 1107, 1111, 1129
FatI CATG 8 cut(s) 113, 469, 640, 981, 987, 1103, 1107, 1125
FokI GGATG 3 cut(s) 737, 863, 968
FspBI CTAG 1 cut(s) 125
GsaI CCCAGC 1 cut(s) 698
HaeIII GGCC 1 cut(s) 539
HapII CCGG 5 cut(s) 95, 541, 867, 1070, 1100
HgaI GACGC 2 cut(s) 44, 53
Hin1II CATG 8 cut(s) 117, 473, 644, 985, 991, 1107, 1111, 1129
HincII GTYRAC 1 cut(s) 736
HindII GTYRAC 1 cut(s) 736
HinfI GANTC 6 cut(s) 91, 152, 821, 1031, 1096, 1111
HpaII CCGG 5 cut(s) 95, 541, 867, 1070, 1100
HphI GGTGA 1 cut(s) 113
Hpy166II GTNNAC 3 cut(s) 736, 763, 776
Hpy188I TCNGA 4 cut(s) 418, 573, 649, 942
Hpy188III TCNNGA 4 cut(s) 95, 286, 1093, 1134
Hpy8I GTNNAC 3 cut(s) 736, 763, 776
HpyAV CCTTC 5 cut(s) 56, 307, 517, 737, 829
HpyCH4III ACNGT 1 cut(s) 233
HpyCH4IV ACGT 2 cut(s) 738, 759
HpyF3I CTNAG 1 cut(s) 342
HpySE526I ACGT 2 cut(s) 738, 759
Hsp92II CATG 8 cut(s) 117, 473, 644, 985, 991, 1107, 1111, 1129
Kpn2I TCCGGA 1 cut(s) 94
Kzo9I GATC 1 cut(s) 928
LmnI GCTCC 2 cut(s) 271, 971
LweI GCATC 3 cut(s) 167, 185, 946
MaeI CTAG 1 cut(s) 125
MaeII ACGT 2 cut(s) 738, 759
MaeIII GTNAC 1 cut(s) 992
MalI GATC 1 cut(s) 930
MboI GATC 1 cut(s) 928
MfeI CAATTG 3 cut(s) 507, 913, 1056
MlyI GAGTC 3 cut(s) 100, 1040, 1090
Mph1103I ATGCAT 1 cut(s) 1109
MroI TCCGGA 1 cut(s) 94
MseI TTAA 1 cut(s) 216
MslI CAYNNNNRTG 3 cut(s) 986, 1070, 1072
MspA1I CMGCKG 3 cut(s) 140, 146, 341
MspI CCGG 5 cut(s) 95, 541, 867, 1070, 1100
MspR9I CCNGG 1 cut(s) 541
MunI CAATTG 3 cut(s) 507, 913, 1056
NciI CCSGG 1 cut(s) 541
NdeII GATC 1 cut(s) 928
NlaIII CATG 8 cut(s) 117, 473, 644, 985, 991, 1107, 1111, 1129
NlaIV GGNNCC 4 cut(s) 646, 663, 748, 967
NsiI ATGCAT 1 cut(s) 1109
NspI RCATGY 2 cut(s) 473, 1107
OliI CACNNNNGTG 2 cut(s) 1070, 1072
PaeI GCATGC 1 cut(s) 1107
PfeI GAWTC 3 cut(s) 152, 821, 1111
Pfl23II CGTACG 1 cut(s) 830
PinAI ACCGGT 2 cut(s) 866, 1069
PleI GAGTC 3 cut(s) 99, 1039, 1090
PpsI GAGTC 3 cut(s) 99, 1039, 1090
Psp1406I AACGTT 1 cut(s) 738
PspEI GGTNACC 1 cut(s) 992
PspFI CCCAGC 1 cut(s) 694
PspLI CGTACG 1 cut(s) 830
PspN4I GGNNCC 4 cut(s) 646, 663, 748, 967
PspPI GGNCC 2 cut(s) 538, 776
PsrI GAACNNNNNNTAC 2 cut(s) 226, 258
PstI CTGCAG 6 cut(s) 145, 151, 310, 322, 340, 382
PstNI CAGNNNCTG 1 cut(s) 377
PvuII CAGCTG 3 cut(s) 140, 146, 341
RsaI GTAC 4 cut(s) 491, 764, 832, 862
RsaNI GTAC 4 cut(s) 490, 763, 831, 861
RseI CAYNNNNRTG 3 cut(s) 986, 1070, 1072
SaqAI TTAA 1 cut(s) 216
Sau3AI GATC 1 cut(s) 928
Sau96I GGNCC 2 cut(s) 538, 776
SchI GAGTC 3 cut(s) 100, 1040, 1090
ScrFI CCNGG 1 cut(s) 541
SfaNI GCATC 3 cut(s) 167, 185, 946
SfcI CTRYAG 7 cut(s) 141, 147, 306, 318, 336, 378, 720
SgrAI CRCCGGYG 1 cut(s) 1069
SinI GGWCC 1 cut(s) 776
SmiMI CAYNNNNRTG 3 cut(s) 986, 1070, 1072
SphI GCATGC 1 cut(s) 1107
SsiI CCGC 1 cut(s) 1005
SspMI CTAG 1 cut(s) 125
StyD4I CCNGG 1 cut(s) 539
TaaI ACNGT 1 cut(s) 233
TaiI ACGT 2 cut(s) 741, 762
TaqI TCGA 2 cut(s) 287, 793
TatI WGTACW 1 cut(s) 489
TauI GCSGC 1 cut(s) 1007
TfiI GAWTC 3 cut(s) 152, 821, 1111
Tru1I TTAA 1 cut(s) 216
Tru9I TTAA 1 cut(s) 216
TscAI CASTG 1 cut(s) 378
TspDTI ATGAA 3 cut(s) 579, 998, 1124
TspGWI ACGGA 1 cut(s) 968
TspRI CASTG 1 cut(s) 378
VpaK11BI GGWCC 1 cut(s) 776
XapI RAATTY 4 cut(s) 212, 289, 586, 633
XceI RCATGY 2 cut(s) 473, 1107
XspI CTAG 1 cut(s) 125
Zsp2I ATGCAT 1 cut(s) 1109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.