MD15G1348200.v1.1

Photosystem II 22 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008089
Physical Location & Seq
Forward (+)
41224378 .. 41227355
2978 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1348200.v1.1.491

Sequence Viewer

Length: 825 bp
ATGGCTCAAACCATGCTTCTCATGTCCAGTAGTGTGTCTTCAGGCCATGTCGTCGACTTGAAGTCCTCAGACCGTTTACTCCAAGTACAAGTTCAAAGACTTAGGCCTAAGTCTTTCTCTCAACTTGTTTTCAGACCCCTCCCATCATCTTCTTTTGCCTCATCATCTTCGACCGTTGTCGCTCTCTTCAAATCGAAAACAAAAGCTCCTGCCAAGAAGGCCCCGTCGCCGAAGCCGAAGGTTGAAGACGGTATCTTTGGAACTTCTGGGGGCATTGGTTTCACAAAGCAGAATGAGCTCTTTGTTGGTCGGGTTGCCATGATTGGCTTTGCTGCATCATTGTTGGGAGAAGCAATTACAGGAAAAGGAATTCTTTCACAATTGAACTTGGAAACCGGAGTTCCCATCTATGAAGCTGAACCACTTCTTCTCTTCTTCATCCTTTTCACCCTCCTTGGGGCCATTGGAGCTTTAGGTGACCGTGGTAAATTCGTCGATGACCCGCCCACTGGAATTGAAGGGGCAGTGATCCCTCCAGGCAAAGGATTTAAATCAGCCTTGGGTCTCAATGAAGGAGGTCCTCTATTTGGATTCACAAAGGCGAACGAGCTATTTGTTGGAAGATTGGCACAGTTGGGATTTGCATTCTCTCTAATAGGAGAAATCATCACAGGGAAGGGAGCTCTAGCCCAGTTAAACATTGAGACTGGAGTCCCCATTAACGAAATCGAACCCCTTGTGTTGCTCAATGTTGTCTTCTTCTTCATTGCTGCAGTGAATCCCGGAACTGGTAAATTCATCACAGATGTGGATGAAGAAGACTAG

Protein Analysis

275

Amino Acids

28.82

Weight (kDa)

6.64

Isoelectric Point (pI)

32.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroa_b-bind PF00504 94 - 218 6.2e-08 Chlorophyll A-B binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 54
AciI CCGC 1 cut(s) 503
AclWI GGATC 1 cut(s) 523
AcsI RAATTY 3 cut(s) 369, 488, 794
AcuI CTGAAG 1 cut(s) 24
AfaI GTAC 1 cut(s) 87
AfiI CCNNNNNNNGG 6 cut(s) 456, 457, 509, 542, 587, 788
AgsI TTSAA 6 cut(s) 61, 95, 190, 245, 385, 518
AhdI GACNNNNNGTC 1 cut(s) 61
AjnI CCWGG 1 cut(s) 535
AjuI GAANNNNNNNTTGG 2 cut(s) 75, 107
AleI CACNNNNGTG 1 cut(s) 806
AluBI AGCT 6 cut(s) 206, 298, 416, 470, 610, 683
AluI AGCT 6 cut(s) 206, 298, 416, 470, 610, 683
Alw21I GWGCWC 2 cut(s) 300, 685
Alw26I GTCTC 2 cut(s) 569, 698
AlwI GGATC 1 cut(s) 523
AoxI GGCC 4 cut(s) 43, 104, 219, 459
ApeKI GCWGC 2 cut(s) 332, 770
ApoI RAATTY 3 cut(s) 369, 488, 794
ArsI GACNNNNNNTTYG 2 cut(s) 239, 271
Asp700I GAANNNNTTC 2 cut(s) 373, 423
AspS9I GGNCC 3 cut(s) 220, 459, 578
AsuC2I CCSGG 1 cut(s) 783
AsuHPI GGTGA 2 cut(s) 439, 488
AvaII GGWCC 1 cut(s) 578
BanII GRGCYC 2 cut(s) 300, 685
BarI GAAGNNNNNNTAC 2 cut(s) 22, 54
BbsI GAAGAC 3 cut(s) 30, 252, 748
Bbv12I GWGCWC 2 cut(s) 300, 685
BbvI GCAGC 2 cut(s) 319, 757
BccI CCATC 2 cut(s) 151, 413
BciT130I CCWGG 1 cut(s) 537
BcnI CCSGG 1 cut(s) 783
BcoDI GTCTC 2 cut(s) 569, 698
BfaI CTAG 2 cut(s) 686, 823
BfmI CTRYAG 1 cut(s) 771
BglI GCCNNNNNGGC 1 cut(s) 218
BisI GCNGC 2 cut(s) 333, 771
BlsI GCNGC 2 cut(s) 334, 772
Bme1390I CCNGG 2 cut(s) 537, 783
Bme18I GGWCC 1 cut(s) 578
BmeRI GACNNNNNGTC 1 cut(s) 61
BmgT120I GGNCC 3 cut(s) 220, 459, 578
BmiI GGNNCC 2 cut(s) 222, 460
BmrFI CCNGG 2 cut(s) 537, 783
BmrI ACTGGG 1 cut(s) 685
BmsI GCATC 1 cut(s) 344
BmuI ACTGGG 1 cut(s) 685
BoxI GACNNNNGTC 2 cut(s) 176, 710
BpiI GAAGAC 3 cut(s) 30, 252, 748
BpmI CTGGAG 2 cut(s) 519, 729
BpuMI CCSGG 1 cut(s) 783
BsaBI GATNNNNATC 1 cut(s) 550
BsaI GGTCTC 1 cut(s) 569
BsaJI CCNNGG 3 cut(s) 454, 481, 558
BsaWI WCCGGW 1 cut(s) 395
BsaXI ACNNNNNCTCC 2 cut(s) 190, 220
Bsc4I CCNNNNNNNGG 6 cut(s) 456, 457, 509, 542, 587, 788
Bse1I ACTGG 5 cut(s) 27, 514, 691, 712, 793
Bse3DI GCAATG 1 cut(s) 765
Bse8I GATNNNNATC 1 cut(s) 550
BseBI CCWGG 1 cut(s) 537
BseDI CCNNGG 3 cut(s) 454, 481, 558
BseGI GGATG 2 cut(s) 438, 817
BseJI GATNNNNATC 1 cut(s) 550
BseLI CCNNNNNNNGG 6 cut(s) 456, 457, 509, 542, 587, 788
BseMI GCAATG 1 cut(s) 765
BseMII CTCAG 1 cut(s) 81
BseNI ACTGG 5 cut(s) 27, 514, 691, 712, 793
BseXI GCAGC 2 cut(s) 319, 757
Bsh1285I CGRYCG 1 cut(s) 174
BshFI GGCC 4 cut(s) 45, 106, 221, 461
BsiEI CGRYCG 1 cut(s) 174
BsiHKAI GWGCWC 2 cut(s) 300, 685
BsiSI CCGG 2 cut(s) 396, 783
BslFI GGGAC 1 cut(s) 698
BslI CCNNNNNNNGG 6 cut(s) 456, 457, 509, 542, 587, 788
BsmAI GTCTC 2 cut(s) 569, 698
BsmFI GGGAC 1 cut(s) 698
BsmI GAATGC 1 cut(s) 644
BsnI GGCC 4 cut(s) 45, 106, 221, 461
Bso31I GGTCTC 1 cut(s) 569
Bsp1286I GDGCHC 2 cut(s) 300, 685
Bsp143I GATC 1 cut(s) 528
BspACI CCGC 1 cut(s) 503
BspANI GGCC 4 cut(s) 45, 106, 221, 461
BspCNI CTCAG 1 cut(s) 80
BspLI GGNNCC 2 cut(s) 222, 460
BspMAI CTGCAG 1 cut(s) 775
BspPI GGATC 1 cut(s) 523
BspTNI GGTCTC 1 cut(s) 569
BsrDI GCAATG 1 cut(s) 765
BsrI ACTGG 5 cut(s) 27, 514, 691, 712, 793
BssECI CCNNGG 3 cut(s) 454, 481, 558
BssMI GATC 1 cut(s) 528
BssT1I CCWWGG 2 cut(s) 454, 558
Bst2UI CCWGG 1 cut(s) 537
Bst4CI ACNGT 5 cut(s) 74, 175, 251, 482, 633
Bst6I CTCTTC 2 cut(s) 191, 437
BstDEI CTNAG 3 cut(s) 67, 101, 108
BstDSI CCRYGG 1 cut(s) 481
BstEII GGTNACC 1 cut(s) 476
BstF5I GGATG 2 cut(s) 438, 817
BstKTI GATC 1 cut(s) 531
BstMAI GTCTC 2 cut(s) 569, 698
BstMBI GATC 1 cut(s) 528
BstMCI CGRYCG 1 cut(s) 174
BstMWI GCNNNNNNNGC 3 cut(s) 218, 295, 467
BstNI CCWGG 1 cut(s) 537
BstPAI GACNNNNGTC 2 cut(s) 176, 710
BstPI GGTNACC 1 cut(s) 476
BstSCI CCNGG 2 cut(s) 535, 781
BstSFI CTRYAG 1 cut(s) 771
BstV1I GCAGC 2 cut(s) 319, 757
BstV2I GAAGAC 3 cut(s) 30, 252, 748
BsuRI GGCC 4 cut(s) 45, 106, 221, 461
BtgI CCRYGG 1 cut(s) 481
BtsCI GGATG 2 cut(s) 438, 817
BtsI GCAGTG 2 cut(s) 531, 780
BtsIMutI CAGTG 3 cut(s) 507, 531, 780
Cfr13I GGNCC 3 cut(s) 220, 459, 578
Csp6I GTAC 1 cut(s) 86
CspCI CAANNNNNGTGG 2 cut(s) 496, 531
CviAII CATG 4 cut(s) 13, 22, 47, 319
CviQI GTAC 1 cut(s) 86
DdeI CTNAG 3 cut(s) 67, 101, 108
DpnI GATC 1 cut(s) 530
DpnII GATC 1 cut(s) 528
DraI TTTAAA 1 cut(s) 550
DriI GACNNNNNGTC 1 cut(s) 61
Eam1104I CTCTTC 2 cut(s) 191, 437
Eam1105I GACNNNNNGTC 1 cut(s) 61
EarI CTCTTC 2 cut(s) 191, 437
Ecl136II GAGCTC 2 cut(s) 298, 683
Eco130I CCWWGG 2 cut(s) 454, 558
Eco147I AGGCCT 1 cut(s) 106
Eco24I GRGCYC 2 cut(s) 300, 685
Eco31I GGTCTC 1 cut(s) 569
Eco47I GGWCC 1 cut(s) 578
Eco53kI GAGCTC 2 cut(s) 298, 683
Eco57I CTGAAG 1 cut(s) 24
Eco91I GGTNACC 1 cut(s) 476
EcoICRI GAGCTC 2 cut(s) 298, 683
EcoO109I RGGNCCY 2 cut(s) 220, 578
EcoO65I GGTNACC 1 cut(s) 476
EcoRI GAATTC 1 cut(s) 369
EcoRII CCWGG 1 cut(s) 535
EcoT14I CCWWGG 2 cut(s) 454, 558
EcoT38I GRGCYC 2 cut(s) 300, 685
ErhI CCWWGG 2 cut(s) 454, 558
FaeI CATG 4 cut(s) 16, 25, 50, 322
FaiI YATR 5 cut(s) 14, 23, 48, 320, 411
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FaqI GGGAC 1 cut(s) 698
FatI CATG 4 cut(s) 12, 21, 46, 318
FauI CCCGC 1 cut(s) 510
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 2 cut(s) 333, 771
FokI GGATG 1 cut(s) 425
FriOI GRGCYC 2 cut(s) 300, 685
Fsp4HI GCNGC 2 cut(s) 333, 771
FspBI CTAG 2 cut(s) 686, 823
GluI GCNGC 2 cut(s) 333, 771
GsuI CTGGAG 2 cut(s) 519, 729
HaeIII GGCC 4 cut(s) 45, 106, 221, 461
HapII CCGG 2 cut(s) 396, 783
Hin1II CATG 4 cut(s) 16, 25, 50, 322
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HinfI GANTC 3 cut(s) 591, 711, 778
HpaII CCGG 2 cut(s) 396, 783
HphI GGTGA 2 cut(s) 439, 488
Hpy166II GTNNAC 2 cut(s) 55, 77
Hpy188I TCNGA 2 cut(s) 70, 134
Hpy8I GTNNAC 2 cut(s) 55, 77
Hpy99I CGWCG 3 cut(s) 56, 229, 497
HpyAV CCTTC 5 cut(s) 211, 232, 512, 566, 670
HpyCH4III ACNGT 5 cut(s) 74, 175, 251, 482, 633
HpyCH4V TGCA 3 cut(s) 335, 644, 773
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 295, 467
HpyF3I CTNAG 3 cut(s) 67, 101, 108
Hsp92II CATG 4 cut(s) 16, 25, 50, 322
Kzo9I GATC 1 cut(s) 528
LmnI GCTCC 3 cut(s) 211, 467, 680
Lsp1109I GCAGC 2 cut(s) 319, 757
LweI GCATC 1 cut(s) 344
MaeI CTAG 2 cut(s) 686, 823
MaeIII GTNAC 1 cut(s) 476
MalI GATC 1 cut(s) 530
MboI GATC 1 cut(s) 528
MfeI CAATTG 1 cut(s) 380
MhlI GDGCHC 2 cut(s) 300, 685
MluCI AATT 6 cut(s) 354, 369, 380, 488, 513, 794
MlyI GAGTC 1 cut(s) 720
MmeI TCCRAC 1 cut(s) 598
MnlI CCTC 7 cut(s) 76, 149, 169, 461, 543, 569, 591
MroXI GAANNNNTTC 2 cut(s) 373, 423
MseI TTAA 3 cut(s) 549, 695, 720
MslI CAYNNNNRTG 1 cut(s) 806
MspI CCGG 2 cut(s) 396, 783
MspR9I CCNGG 2 cut(s) 537, 783
MunI CAATTG 1 cut(s) 380
Mva1269I GAATGC 1 cut(s) 644
MvaI CCWGG 1 cut(s) 537
MwoI GCNNNNNNNGC 3 cut(s) 218, 295, 467
NciI CCSGG 1 cut(s) 783
NdeII GATC 1 cut(s) 528
NlaIII CATG 4 cut(s) 16, 25, 50, 322
NlaIV GGNNCC 2 cut(s) 222, 460
NmuCI GTSAC 1 cut(s) 476
OliI CACNNNNGTG 1 cut(s) 806
PceI AGGCCT 1 cut(s) 106
PcsI WCGNNNNNNNCGW 1 cut(s) 233
PctI GAATGC 1 cut(s) 644
PdmI GAANNNNTTC 2 cut(s) 373, 423
PfeI GAWTC 2 cut(s) 591, 778
PfoI TCCNGGA 1 cut(s) 781
PkrI GCNGC 2 cut(s) 334, 772
PleI GAGTC 1 cut(s) 719
PpsI GAGTC 1 cut(s) 719
PpuMI RGGWCCY 1 cut(s) 578
PshAI GACNNNNGTC 2 cut(s) 176, 710
Psp124BI GAGCTC 2 cut(s) 300, 685
Psp5II RGGWCCY 1 cut(s) 578
Psp6I CCWGG 1 cut(s) 535
PspEI GGTNACC 1 cut(s) 476
PspGI CCWGG 1 cut(s) 535
PspN4I GGNNCC 2 cut(s) 222, 460
PspPI GGNCC 3 cut(s) 220, 459, 578
PspPPI RGGWCCY 1 cut(s) 578
PstI CTGCAG 1 cut(s) 775
RsaI GTAC 1 cut(s) 87
RsaNI GTAC 1 cut(s) 86
RseI CAYNNNNRTG 1 cut(s) 806
SacI GAGCTC 2 cut(s) 300, 685
SalI GTCGAC 1 cut(s) 53
SaqAI TTAA 3 cut(s) 549, 695, 720
SatI GCNGC 2 cut(s) 333, 771
Sau3AI GATC 1 cut(s) 528
Sau96I GGNCC 3 cut(s) 220, 459, 578
SchI GAGTC 1 cut(s) 720
ScrFI CCNGG 2 cut(s) 537, 783
SduI GDGCHC 2 cut(s) 300, 685
SetI ASST 9 cut(s) 208, 243, 300, 418, 472, 478, 580, 612, 685
SfaNI GCATC 1 cut(s) 344
SfcI CTRYAG 1 cut(s) 771
SinI GGWCC 1 cut(s) 578
SmiI ATTTAAAT 1 cut(s) 550
SmiMI CAYNNNNRTG 1 cut(s) 806
Sse9I AATT 6 cut(s) 354, 369, 380, 488, 513, 794
SseBI AGGCCT 1 cut(s) 106
SsiI CCGC 1 cut(s) 503
SspMI CTAG 2 cut(s) 686, 823
SstI GAGCTC 2 cut(s) 300, 685
StuI AGGCCT 1 cut(s) 106
StyD4I CCNGG 2 cut(s) 535, 781
StyI CCWWGG 2 cut(s) 454, 558
SwaI ATTTAAAT 1 cut(s) 550
TaaI ACNGT 5 cut(s) 74, 175, 251, 482, 633
TaqI TCGA 5 cut(s) 54, 170, 194, 495, 729
TasI AATT 6 cut(s) 354, 369, 380, 488, 513, 794
TatI WGTACW 1 cut(s) 85
TfiI GAWTC 2 cut(s) 591, 778
Tru1I TTAA 3 cut(s) 549, 695, 720
Tru9I TTAA 3 cut(s) 549, 695, 720
TscAI CASTG 3 cut(s) 514, 531, 780
TseFI GTSAC 1 cut(s) 476
TseI GCWGC 2 cut(s) 332, 770
Tsp45I GTSAC 1 cut(s) 476
TspDTI ATGAA 5 cut(s) 426, 427, 585, 754, 787
TspRI CASTG 3 cut(s) 514, 531, 780
VpaK11BI GGWCC 1 cut(s) 578
XapI RAATTY 3 cut(s) 369, 488, 794
XmiI GTMKAC 1 cut(s) 54
XmnI GAANNNNTTC 2 cut(s) 373, 423
XspI CTAG 2 cut(s) 686, 823
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.