Rorug06G0254300

Photosystem II 22 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0008089
Physical Location & Seq
Reverse (-)
40077172 .. 40078752
1581 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0254300.1

Sequence Viewer

Length: 990 bp
ATGGAAACTAGCACCACTCAGAATGGTAGTAGTAGTATCATGTGGTTCTTCAGGGATAAAGGGTTTGATGATAAGAGTATTAGTGAAATGTTCAAAAAATGCAAGCGTCTTGAGAATGTGAATAGGGAAAGTGCCTCTGAGAATTGGGCTTATCTGGCAAGTATTGGAATTCAAGAGAGGAAGCTACCTTCTGTTGTGTCGAAGTGCCCCAAGATACTTACTTTAGGTCTTCATGAGAAGCTTGTACCTACGGTCGAGTGCCTGACCACGCTTGGCACAAAACCGCGTGAAGTTGCGTCCGCCATTGCCAAATTCCCTCACATTCTGTCGCATAGTGTGGAAGAGAAGCTCTGTCCACTGTTGGCTTTCTTTGAGGCATTCGGTGTTCCTCAGAAGCAACTTGGCAAAATGATTTTGCTGAATCCTAGGCTCATCAGTTACAGCATTGAAACAAAGCTGAAGGACATTGTGGACTTTCTTGCTGATCTTGGCCTTGCCAGAGATGGGATGATTGGCAAAGTACTGGTGAAGAACCCATTTATCATGGGTTATAGTGTTGATAAAAGACTGCGGCCTACTGCAGAGTTTTTGAAATCCTTTGGTCTCACAGACCAGGGTATTCAGACAGTGGCAATACACTTTCCAGAAGTCCTGTGTAGGGATGTGGACAAGATTTTGAGACCCAATTTCGATTTTTTGAAAAGATCTGGATTTGAAGATGGGCAGATAGCAGCTCTGGTGACTGGTTATCCTCCCATTTTGATCAAAAGCATCCACAATTCTTTAGAACCCAGAATCAAGTTTTTGGTAGAGGTAATGGGAAGACGAATTGATGAAGTCACCGATTATCCTGACTTCTTTCGCCATGGTTTGAAGAAAAAAGTGGAGCGGCGACACAAACTGTTGAAACAGACGACGACTGATTGCAGCTTAAGTGAAATGCTGGACTGCAATCAAAAGAAGTTCCTATTGAAGTTTGGTTTGGCTTGA

Protein Analysis

329

Amino Acids

37.33

Weight (kDa)

9.24

Isoelectric Point (pI)

32.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mTERF PF02536 16 - 323 2.4e-58 mTERF
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 889
AccII CGCG 1 cut(s) 286
AciI CCGC 4 cut(s) 284, 300, 571, 889
AcsI RAATTY 2 cut(s) 168, 311
AcuI CTGAAG 2 cut(s) 34, 479
AfaI GTAC 2 cut(s) 246, 522
AfiI CCNNNNNNNGG 2 cut(s) 504, 658
AflII CTTAAG 1 cut(s) 931
AgsI TTSAA 9 cut(s) 94, 173, 449, 592, 700, 716, 874, 907, 973
AjnI CCWGG 1 cut(s) 612
AjuI GAANNNNNNNTTGG 1 cut(s) 965
AluBI AGCT 6 cut(s) 184, 241, 349, 457, 734, 930
AluI AGCT 6 cut(s) 184, 241, 349, 457, 734, 930
Alw26I GTCTC 2 cut(s) 608, 673
AoxI GGCC 2 cut(s) 490, 572
ApeKI GCWGC 2 cut(s) 731, 927
ApoI RAATTY 2 cut(s) 168, 311
AspA2I CCTAGG 1 cut(s) 425
AsuHPI GGTGA 3 cut(s) 538, 751, 832
AvrII CCTAGG 1 cut(s) 425
BaeGI GKGCMC 1 cut(s) 209
BbsI GAAGAC 2 cut(s) 221, 829
BbvI GCAGC 2 cut(s) 743, 939
BccI CCATC 2 cut(s) 497, 713
BciT130I CCWGG 1 cut(s) 614
BclI TGATCA 1 cut(s) 762
BcoDI GTCTC 2 cut(s) 608, 673
BfaI CTAG 2 cut(s) 9, 426
BfmI CTRYAG 1 cut(s) 579
BfrI CTTAAG 1 cut(s) 931
BglII AGATCT 1 cut(s) 704
BisI GCNGC 4 cut(s) 572, 732, 890, 928
BlnI CCTAGG 1 cut(s) 425
BlsI GCNGC 4 cut(s) 573, 733, 891, 929
BmcAI AGTACT 1 cut(s) 522
Bme1390I CCNGG 1 cut(s) 614
BmrFI CCNGG 1 cut(s) 614
BmsI GCATC 1 cut(s) 780
BpiI GAAGAC 2 cut(s) 221, 829
BpuEI CTTGAG 1 cut(s) 131
BsaI GGTCTC 2 cut(s) 608, 673
BsaJI CCNNGG 3 cut(s) 425, 613, 865
Bsc4I CCNNNNNNNGG 2 cut(s) 504, 658
Bse1I ACTGG 2 cut(s) 528, 748
Bse3DI GCAATG 1 cut(s) 303
BseBI CCWGG 1 cut(s) 614
BseDI CCNNGG 3 cut(s) 425, 613, 865
BseGI GGATG 3 cut(s) 513, 667, 771
BseLI CCNNNNNNNGG 2 cut(s) 504, 658
BseMI GCAATG 1 cut(s) 303
BseMII CTCAG 3 cut(s) 32, 129, 404
BseNI ACTGG 2 cut(s) 528, 748
BseSI GKGCMC 1 cut(s) 209
BseXI GCAGC 2 cut(s) 743, 939
Bsh1236I CGCG 1 cut(s) 286
Bsh1285I CGRYCG 1 cut(s) 255
BshFI GGCC 2 cut(s) 492, 574
BsiEI CGRYCG 1 cut(s) 255
BslI CCNNNNNNNGG 2 cut(s) 504, 658
BsmAI GTCTC 2 cut(s) 608, 673
BsmI GAATGC 1 cut(s) 377
BsnI GGCC 2 cut(s) 492, 574
Bso31I GGTCTC 2 cut(s) 608, 673
Bsp1286I GDGCHC 1 cut(s) 209
Bsp143I GATC 3 cut(s) 484, 704, 762
Bsp19I CCATGG 1 cut(s) 865
BspACI CCGC 4 cut(s) 284, 300, 571, 889
BspANI GGCC 2 cut(s) 492, 574
BspCNI CTCAG 3 cut(s) 31, 130, 403
BspFNI CGCG 1 cut(s) 286
BspHI TCATGA 1 cut(s) 232
BspMAI CTGCAG 1 cut(s) 583
BspTI CTTAAG 1 cut(s) 931
BspTNI GGTCTC 2 cut(s) 608, 673
BsrBI CCGCTC 1 cut(s) 889
BsrDI GCAATG 1 cut(s) 303
BsrI ACTGG 2 cut(s) 528, 748
BssECI CCNNGG 3 cut(s) 425, 613, 865
BssMI GATC 3 cut(s) 484, 704, 762
BssT1I CCWWGG 2 cut(s) 425, 865
Bst2UI CCWGG 1 cut(s) 614
Bst4CI ACNGT 4 cut(s) 253, 360, 628, 903
Bst6I CTCTTC 1 cut(s) 336
BstAFI CTTAAG 1 cut(s) 931
BstC8I GCNNGC 1 cut(s) 104
BstDEI CTNAG 3 cut(s) 18, 138, 390
BstDSI CCRYGG 1 cut(s) 865
BstF5I GGATG 3 cut(s) 513, 667, 771
BstFNI CGCG 1 cut(s) 286
BstKTI GATC 3 cut(s) 487, 707, 765
BstMAI GTCTC 2 cut(s) 608, 673
BstMBI GATC 3 cut(s) 484, 704, 762
BstMCI CGRYCG 1 cut(s) 255
BstMWI GCNNNNNNNGC 1 cut(s) 155
BstNI CCWGG 1 cut(s) 614
BstSCI CCNGG 1 cut(s) 612
BstSFI CTRYAG 1 cut(s) 579
BstSLI GKGCMC 1 cut(s) 209
BstUI CGCG 1 cut(s) 286
BstV1I GCAGC 2 cut(s) 743, 939
BstV2I GAAGAC 2 cut(s) 221, 829
BstX2I RGATCY 1 cut(s) 704
BstYI RGATCY 1 cut(s) 704
BsuRI GGCC 2 cut(s) 492, 574
BtgI CCRYGG 1 cut(s) 865
BtsCI GGATG 3 cut(s) 513, 667, 771
BtsIMutI CAGTG 2 cut(s) 356, 633
Cac8I GCNNGC 1 cut(s) 104
CciI TCATGA 1 cut(s) 232
CseI GACGC 2 cut(s) 95, 285
Csp6I GTAC 2 cut(s) 245, 521
CviAII CATG 4 cut(s) 40, 233, 544, 866
CviQI GTAC 2 cut(s) 245, 521
DdeI CTNAG 3 cut(s) 18, 138, 390
DpnI GATC 3 cut(s) 486, 706, 764
DpnII GATC 3 cut(s) 484, 704, 762
Eam1104I CTCTTC 1 cut(s) 336
EarI CTCTTC 1 cut(s) 336
EciI GGCGGA 1 cut(s) 289
Eco130I CCWWGG 2 cut(s) 425, 865
Eco31I GGTCTC 2 cut(s) 608, 673
Eco57I CTGAAG 2 cut(s) 34, 479
EcoRI GAATTC 1 cut(s) 168
EcoRII CCWGG 1 cut(s) 612
EcoT14I CCWWGG 2 cut(s) 425, 865
ErhI CCWWGG 2 cut(s) 425, 865
FaeI CATG 4 cut(s) 43, 236, 547, 869
FaiI YATR 6 cut(s) 41, 234, 333, 545, 552, 867
FatI CATG 4 cut(s) 39, 232, 543, 865
FbaI TGATCA 1 cut(s) 762
Fnu4HI GCNGC 4 cut(s) 572, 732, 890, 928
FokI GGATG 3 cut(s) 520, 674, 758
Fsp4HI GCNGC 4 cut(s) 572, 732, 890, 928
FspBI CTAG 2 cut(s) 9, 426
GluI GCNGC 4 cut(s) 572, 732, 890, 928
HaeIII GGCC 2 cut(s) 492, 574
HgaI GACGC 2 cut(s) 95, 285
Hin1II CATG 4 cut(s) 43, 236, 547, 869
HindIII AAGCTT 1 cut(s) 239
HinfI GANTC 2 cut(s) 421, 795
HphI GGTGA 3 cut(s) 538, 751, 832
Hpy166II GTNNAC 3 cut(s) 356, 472, 667
Hpy188I TCNGA 4 cut(s) 21, 139, 393, 624
Hpy188III TCNNGA 6 cut(s) 110, 173, 233, 644, 708, 851
Hpy8I GTNNAC 3 cut(s) 356, 472, 667
Hpy99I CGWCG 1 cut(s) 919
HpyAV CCTTC 2 cut(s) 198, 454
HpyCH4III ACNGT 4 cut(s) 253, 360, 628, 903
HpyCH4V TGCA 4 cut(s) 102, 581, 927, 951
HpyF10VI GCNNNNNNNGC 1 cut(s) 155
HpyF3I CTNAG 3 cut(s) 18, 138, 390
Hsp92II CATG 4 cut(s) 43, 236, 547, 869
Ksp22I TGATCA 1 cut(s) 762
Kzo9I GATC 3 cut(s) 484, 704, 762
LmnI GCTCC 1 cut(s) 886
Lsp1109I GCAGC 2 cut(s) 743, 939
LweI GCATC 1 cut(s) 780
MaeI CTAG 2 cut(s) 9, 426
MaeIII GTNAC 3 cut(s) 437, 739, 838
MalI GATC 3 cut(s) 486, 706, 764
MbiI CCGCTC 1 cut(s) 889
MboI GATC 3 cut(s) 484, 704, 762
MboII GAAGA 7 cut(s) 40, 221, 353, 541, 728, 834, 886
MflI RGATCY 1 cut(s) 704
MhlI GDGCHC 1 cut(s) 209
MluCI AATT 6 cut(s) 142, 168, 311, 685, 778, 828
MnlI CCTC 7 cut(s) 145, 171, 327, 367, 399, 762, 805
MseI TTAA 1 cut(s) 932
MspCI CTTAAG 1 cut(s) 931
MspR9I CCNGG 1 cut(s) 614
Mva1269I GAATGC 1 cut(s) 377
MvaI CCWGG 1 cut(s) 614
MvnI CGCG 1 cut(s) 286
MwoI GCNNNNNNNGC 1 cut(s) 155
NcoI CCATGG 1 cut(s) 865
NdeII GATC 3 cut(s) 484, 704, 762
NlaIII CATG 4 cut(s) 43, 236, 547, 869
NmuCI GTSAC 2 cut(s) 739, 838
PagI TCATGA 1 cut(s) 232
PctI GAATGC 1 cut(s) 377
PfeI GAWTC 2 cut(s) 421, 795
PkrI GCNGC 4 cut(s) 573, 733, 891, 929
Psp6I CCWGG 1 cut(s) 612
PspGI CCWGG 1 cut(s) 612
PstI CTGCAG 1 cut(s) 583
PsuI RGATCY 1 cut(s) 704
RsaI GTAC 2 cut(s) 246, 522
RsaNI GTAC 2 cut(s) 245, 521
SaqAI TTAA 1 cut(s) 932
SatI GCNGC 4 cut(s) 572, 732, 890, 928
Sau3AI GATC 3 cut(s) 484, 704, 762
ScaI AGTACT 1 cut(s) 522
ScrFI CCNGG 1 cut(s) 614
SduI GDGCHC 1 cut(s) 209
SfaNI GCATC 1 cut(s) 780
SfcI CTRYAG 1 cut(s) 579
SmlI CTYRAG 2 cut(s) 110, 931
SmoI CTYRAG 2 cut(s) 110, 931
Sse9I AATT 6 cut(s) 142, 168, 311, 685, 778, 828
SsiI CCGC 4 cut(s) 284, 300, 571, 889
SspMI CTAG 2 cut(s) 9, 426
StyD4I CCNGG 1 cut(s) 612
StyI CCWWGG 2 cut(s) 425, 865
TaaI ACNGT 4 cut(s) 253, 360, 628, 903
TaqI TCGA 3 cut(s) 200, 255, 690
TasI AATT 6 cut(s) 142, 168, 311, 685, 778, 828
TatI WGTACW 1 cut(s) 520
TauI GCSGC 2 cut(s) 574, 892
TfiI GAWTC 2 cut(s) 421, 795
Tru1I TTAA 1 cut(s) 932
Tru9I TTAA 1 cut(s) 932
TscAI CASTG 2 cut(s) 363, 633
TseFI GTSAC 2 cut(s) 739, 838
TseI GCWGC 2 cut(s) 731, 927
Tsp45I GTSAC 2 cut(s) 739, 838
TspDTI ATGAA 2 cut(s) 221, 849
TspRI CASTG 2 cut(s) 363, 633
Vha464I CTTAAG 1 cut(s) 931
XapI RAATTY 2 cut(s) 168, 311
XmaJI CCTAGG 1 cut(s) 425
XspI CTAG 2 cut(s) 9, 426
ZrmI AGTACT 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.