Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0002761

Physical Location & Seq

Genomic Coordinates Forward (+)

3151185 .. 3158440

Gene Structure 7256 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1044600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3033 bp

ATGACCAGCGACGCCCAGGACTCGCATCGCAAGCACCGCGGTGCCTCTTCCGATGACGAAGAGGCCGTCGAGAAGTCGTCCAAGCGCCACAAGCACCGCCACCACCGCCGCCACCATCGTCACCGTCACGGCAGCAAAAAGCGCGAAGAGGAGACCAAGGCCGTCGTCGACGATGTCGAAGCTCCTCCACCGCCGCCTGTTGAGTTATATGATTCCGGTGTCAATGGTTCTATGCCGGTTGAAGACGTGGAGGAAGGCGAAATTCTCGACGAGGAGGGTGTCGGTGGTCGCGATGATGGGGTTTCCAATGCGGAGTCTGGTGAAATTGAGACTCCTGGAGTTGGCGATCATTCTAATAAGCGGAATCTGAGAGACAATAACATCAATAAAAATACAAAGACTGTGGGACACCTGCATGATGATGATGATGATGATGATGATGCCCGCGGTCGTGTTGGTGGCAAGTCTCGGAATGAGGCTGGTTTGCCTAGAGATTCTAGGCGACACAGGGAGTCTGGGTCTCCTTCTAGAGGAAGTGATAAGAGAAAGAATTACTTTGATGATGAAAAAGTGTATGAAGATGGAAGTAAATCAGCTAATGGGAAAAAGTTATCCTCTGGAAGTAGTGGGGACAAGTACAATACCTTGGTGCGTTCACCATCTTGTGATAGGCGCGGTGACGAGCTACATGGTAGGGGCAGATCAAGGTCGTATGACCGTTTGAGGGAGAGATCTCGTTCCCGCAGTATAATAGAAGAAGAGTCTCTTTCGAAGAGGAGACATTATCATGAACGGGACACCTCTGTGTATGTTGAGAAAAGCAAGGCTGTGCACGGCTCTGATGATGAAGGATTGACTCCTGGTATTGACAGTGTTGATCTGAGGAGGGATGATGAAAGGGAACACAATATTAGTTATAGCAGATACTCTGGATTAGGCCATAGTCGAGAAAGAGAGAGAAGTAGGGAGAGGGAGGTGGGTCAGGAGCAGAGAAGGGAGAAAGATCAAGAAAGAATCAGGGACAATGAACGGAGAAGGGAGAAAGAAAGAGAAAGGAGTAGTGACAGGGATTTGGTTAGGGATAGGAAAAGGGGAATAGGACGAGAGCATAGCAGGGAAAGAGTGATTTATATGGAAAGGAGAAGGGAAGAGCGACATAGGAGCAGGGACATTGAGAATAGGGACAGGAATAGGGATGATAGTAATAGGAGCAGGGACAGGGTCAAGGATGATAGGGACAGAAACATGGAGAGGGGCCGAGATAGGGCCAAAGATACAGACCGAAAATGGGAGAGGCGAGATGATAGAGACAGGGATAGGGGTCGAGATATGGAGAGGGAGAAATATGAGCATCATGAGGATATCTATGGAAACCGAGATAGGCATAAATCTGCTCGATCAAGGCACAATGGAAAAGGATACGACCAGGACCGAAGAAGTAAAACTGATCCAGCAAAGGTACAGAGTCCAAAGAATTATTCTGTCGAAGGGGATGAAGACATGTTAGAAAGAGATGAAGATGAAGAAGAAAGCATTCAAGTTGCTGGCCAGGAAGAGGATGATGTGGATAGAATCAAGGAAGAGTCTCGAAGAATAAAACAAGCCATCTTAGAAAAATATAAAAGTCAGCAGTCACAGCAAACAAAAGAGTTGCGTCTACAAGAACAAACTGAGTCACAGTTGCAAAAGCAATTTGAGCCTCAGCCTCTGGAGCAATCAGTGGATAAAGGTTCTGCAGATCATCTGGAGCAATCAGCAGCTTCTGTCAAACCTGGTCTAGAAACTGATGATGGAAGGAGTGATGGGGATGTGTCTGTTGCTGCATTTTCTGTTTCACCTCAGCAGAATGGTGCTGATACTTCTAGAATGCCTTCTGGTGCCACGGGGCTTGGAGAGGGTACTCCAGAGAGTGAAGGAGCTGTTGCCGTTGATGATATCTTTGGTGAGACCCCAGTTGGAGTTCAAAAATTGGGAAAAGGAGATCGTGTACAAATTGAAAGGAGTGGTATTCATGATAACTGGGATGATGCAGAAGGGTACTACAGTTACCGGTTTGGAGAATTACTTGATGCTCGATATGAAATCACTGCTGCTCATGGGAAGGGTGTCTTTTCGACAGTCGTCCGTGCAAAGAACCTTACAGTTGGTAATGGTGAACCTGAAGAAGTGGCAATTAAGATGATACGGAGCAATGACACTATGTTAAAGGCCGGTATGACTGAGTTGACCATATTGAAGAAATTAGCAGGTCACGATCCAGATAATAAGCGCCACTGTGTTCGTTTTCTGTCGAATTTCAAGTATCGAAATCATCTTTGTTTAGTGTTTGAATCCCTTCATATGAATCTTCGTGAGGTCTTAAAGAAGTTTGGGCGCAATATTGGTCTTAAGCTAACTGGCGTCAGAGAATATGCTAAGCAACTTTTTATTGCACTGAAGCATTTAAAGAACTGCGGTGTCCTTCATTGTGATATAAAGCCAGATAACATGCTGGTAAATGAGTCAAAAAATGTATTGAAGCTGTGTGATTTTGGTAATGCCATGTTCGCTGGAAAGAATGAAGTTACACCATACCTCGTCAGCCGCTTTTATCGTGCTCCTGAAATAATTCTTGGCTTGCCTTATGATCATCCACTGGATATCTGGTCAGTTGGTTGCTGTTTGTACGAACTTTATACAGGAAAAGTTCTTTTTCCCGGTGCTACAAACAATGATATGCTACGTCTACACATGGAACTGAAAGGCCCTTTCCCAAAGAAGATGCTTCGTAAGGGAGCTTTTACACATCAACATTTTGATCAGGATCTGAACTTCCATGCTACAGAAGATGATCCTGTTACAAAGAAGGCAATCAATCGGGTGATTTTTAATATAAAGCTAAAAGATATTGGATCACTTATTACGGGTTCTGGTGAGAATGCAAAGATGCTAGCTGACTTTAAAGATCTTTTAGATAAAATGTTTGTATTGGATCCAGATAAGAGGTTGACAGTATCACAAGCTTTGAACCATCCATTCATCACTGGCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016310 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase TBPIP

Protein Analysis

1011

Amino Acids

115.97

Weight (kDa)

6.69

Isoelectric Point (pI)

55.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	694 - 1007	6e-48	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	699 - 909	1e-20	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002761)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13350 AT1G13350 AT1G13350 AT3G25840 AT3G25840 AT3G53640
fragaria_vesca	FvH4_4g27640 FvH4_4g27640 FvH4_4g27640 FvH4_4g27640 FvH4_4g27640 FvH4_4g27640 FvH4_4g27640
malus_domestica	MD13G1043800.v1.1 MD16G1044600.v1.1
prunus_persica	Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1
pyrus_communis	pycom16g03900
rosa_chinensis	RchiOBHm_Chr4g0435481
rosa_laevigata	RLG00000006589
rosa_multiflora	Rmu_sc0001940.1_g000027
rosa_roxburghii	Rroxscaffold_5G00376710
rosa_rugosa	Rorug04G0285400 Rorug04G0285500
rosa_samantha	Rh4AG341100 Rh4BG349300 Rh4CG364000 Rh4DG343600
rosa_wichuraiana	Rw4G029760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	420
Acc36I	ACCTGC	2 cut(s)	420, 2237
AccB1I	GGYRCC	2 cut(s)	41, 1877
AccI	GTMKAC	3 cut(s)	168, 1657, 2725
AccII	CGCG	5 cut(s)	39, 144, 291, 447, 675
AclWI	GGATC	7 cut(s)	1442, 2249, 2811, 2825, 2899, 2966, 2979
AcoI	YGGCCR	1 cut(s)	1546
AcsI	RAATTY	2 cut(s)	261, 2293
AcuI	CTGAAG	2 cut(s)	2181, 2456
AcyI	GRCGYC	2 cut(s)	12, 2400
AfaI	GTAC	6 cut(s)	638, 1461, 1900, 1989, 2039, 2666
AfiI	CCNNNNNNNGG	6 cut(s)	341, 530, 724, 1264, 1288, 1555
AflII	CTTAAG	1 cut(s)	2387
AflIII	ACRYGT	1 cut(s)	1500
AgeI	ACCGGT	1 cut(s)	2049
AgsI	TTSAA	9 cut(s)	242, 1538, 1964, 1997, 2236, 2299, 2330, 2518, 3007
AjiI	CACGTC	1 cut(s)	247
AjnI	CCWGG	6 cut(s)	15, 334, 859, 1425, 1548, 1771
AjuI	GAANNNNNNNTTGG	4 cut(s)	1462, 1494, 2595, 2627
AleI	CACNNNNGTG	2 cut(s)	39, 803
Alw21I	GWGCWC	2 cut(s)	834, 2599
Alw44I	GTGCAC	1 cut(s)	830
AlwI	GGATC	7 cut(s)	1442, 2249, 2811, 2825, 2899, 2966, 2979
AlwNI	CAGNNNCTG	3 cut(s)	1708, 1763, 2806
AoxI	GGCC	8 cut(s)	63, 159, 937, 1255, 1266, 1546, 2208, 2743
ApaLI	GTGCAC	1 cut(s)	830
ApeKI	GCWGC	4 cut(s)	132, 1757, 1820, 2090
ApoI	RAATTY	2 cut(s)	261, 2293
ArsI	GACNNNNNNTTYG	2 cut(s)	51, 83
AsiGI	ACCGGT	1 cut(s)	2049
Asp700I	GAANNNNTTC	4 cut(s)	1533, 1870, 2334, 2607
AspLEI	GCGC	5 cut(s)	87, 144, 675, 2271, 2376
AspS9I	GGNCC	4 cut(s)	1255, 1266, 1429, 2744
AsuC2I	CCSGG	1 cut(s)	2697
AsuHPI	GGTGA	9 cut(s)	113, 332, 648, 689, 1827, 1955, 2165, 2872, 2924
AsuII	TTCGAA	1 cut(s)	770
AsuNHI	GCTAGC	1 cut(s)	2929
AvaII	GGWCC	1 cut(s)	1429
BaeGI	GKGCMC	1 cut(s)	834
BaeI	ACNNNNGTAYC	2 cut(s)	2029, 2062
BalI	TGGCCA	1 cut(s)	1548
BamHI	GGATCC	1 cut(s)	2971
BanI	GGYRCC	2 cut(s)	41, 1877
BbsI	GAAGAC	2 cut(s)	249, 1503
Bbv12I	GWGCWC	2 cut(s)	834, 2599
BbvCI	CCTCAGC	2 cut(s)	1701, 1839
BbvI	GCAGC	4 cut(s)	144, 1769, 1807, 2077
BccI	CCATC	8 cut(s)	123, 290, 575, 667, 1613, 1784, 1796, 3018
BceAI	ACGGC	5 cut(s)	50, 145, 146, 850, 1910
BciT130I	CCWGG	6 cut(s)	17, 336, 861, 1427, 1550, 1773
BciVI	GTATCC	1 cut(s)	1412
BclI	TGATCA	2 cut(s)	2626, 2797
BcnI	CCSGG	1 cut(s)	2697
BfaI	CTAG	6 cut(s)	489, 498, 528, 1778, 1863, 2930
BfmI	CTRYAG	3 cut(s)	1734, 2041, 2820
BfoI	RGCGCY	2 cut(s)	88, 2272
BfrI	CTTAAG	1 cut(s)	2387
BfuAI	ACCTGC	2 cut(s)	420, 2237
BfuI	GTATCC	1 cut(s)	1412
BglII	AGATCT	2 cut(s)	731, 2944
BisI	GCNGC	7 cut(s)	109, 133, 194, 1758, 1821, 2091, 2584
BlpI	GCTNAGC	1 cut(s)	2415
BlsI	GCNGC	7 cut(s)	110, 134, 195, 1759, 1822, 2092, 2585
Bme1390I	CCNGG	7 cut(s)	17, 336, 861, 1427, 1550, 1773, 2697
Bme18I	GGWCC	1 cut(s)	1429
BmgBI	CACGTC	1 cut(s)	247
BmgT120I	GGNCC	4 cut(s)	1255, 1266, 1429, 2744
BmiI	GGNNCC	4 cut(s)	43, 1256, 1879, 2973
BmrFI	CCNGG	7 cut(s)	17, 336, 861, 1427, 1550, 1773, 2697
BmrI	ACTGGG	2 cut(s)	1946, 2029
BmsI	GCATC	7 cut(s)	34, 430, 1360, 2017, 2059, 2751, 2916
BmtI	GCTAGC	1 cut(s)	2933
BmuI	ACTGGG	2 cut(s)	1946, 2029
BoxI	GACNNNNGTC	1 cut(s)	2120
BpiI	GAAGAC	2 cut(s)	249, 1503
BpmI	CTGGAG	4 cut(s)	357, 1730, 1766, 1887
Bpu10I	CCTNAGC	2 cut(s)	1701, 1839
Bpu1102I	GCTNAGC	1 cut(s)	2415
Bpu14I	TTCGAA	1 cut(s)	770
BpuMI	CCSGG	1 cut(s)	2697
BsaBI	GATNNNNATC	1 cut(s)	2802
BsaHI	GRCGYC	2 cut(s)	12, 2400
BsaI	GGTCTC	3 cut(s)	146, 525, 1940
BsaJI	CCNNGG	6 cut(s)	15, 37, 156, 445, 645, 1881
BsaWI	WCCGGW	2 cut(s)	215, 2049
BsaXI	ACNNNNNCTCC	4 cut(s)	503, 533, 1971, 2001
Bsc4I	CCNNNNNNNGG	6 cut(s)	341, 530, 724, 1264, 1288, 1555
Bse118I	RCCGGY	3 cut(s)	235, 2049, 2210
Bse1I	ACTGG	5 cut(s)	1952, 2024, 2401, 2640, 3028
Bse3DI	GCAATG	1 cut(s)	2197
Bse8I	GATNNNNATC	1 cut(s)	2802
BseBI	CCWGG	6 cut(s)	17, 336, 861, 1427, 1550, 1773
BseDI	CCNNGG	6 cut(s)	15, 37, 156, 445, 645, 1881
BseGI	GGATG	9 cut(s)	895, 1201, 1234, 1498, 1564, 1813, 2029, 2629, 3010
BseJI	GATNNNNATC	1 cut(s)	2802
BseLI	CCNNNNNNNGG	6 cut(s)	341, 530, 724, 1264, 1288, 1555
BseMI	GCAATG	1 cut(s)	2197
BseMII	CTCAG	6 cut(s)	359, 872, 1662, 1715, 1853, 2211
BseNI	ACTGG	5 cut(s)	1952, 2024, 2401, 2640, 3028
BseRI	GAGGAG	5 cut(s)	164, 174, 287, 790, 898
BseSI	GKGCMC	1 cut(s)	834
BseXI	GCAGC	4 cut(s)	144, 1769, 1807, 2077
Bsh1236I	CGCG	5 cut(s)	39, 144, 291, 447, 675
Bsh1285I	CGRYCG	1 cut(s)	451
BshFI	GGCC	8 cut(s)	65, 161, 939, 1257, 1268, 1548, 2210, 2745
BshNI	GGYRCC	2 cut(s)	41, 1877
BshTI	ACCGGT	1 cut(s)	2049
BsiEI	CGRYCG	1 cut(s)	451
BsiHKAI	GWGCWC	2 cut(s)	834, 2599
BsiSI	CCGG	5 cut(s)	216, 236, 2050, 2211, 2697
BslFI	GGGAC	8 cut(s)	420, 644, 809, 1034, 1181, 1196, 1229, 1250
BslI	CCNNNNNNNGG	6 cut(s)	341, 530, 724, 1264, 1288, 1555
BsmFI	GGGAC	8 cut(s)	420, 644, 809, 1034, 1181, 1196, 1229, 1250
BsmI	GAATGC	3 cut(s)	1533, 1872, 2923
BsnI	GGCC	8 cut(s)	65, 161, 939, 1257, 1268, 1548, 2210, 2745
Bso31I	GGTCTC	3 cut(s)	146, 525, 1940
Bsp119I	TTCGAA	1 cut(s)	770
Bsp1286I	GDGCHC	2 cut(s)	834, 2599
Bsp1407I	TGTACA	1 cut(s)	1987
Bsp1720I	GCTNAGC	1 cut(s)	2415
Bsp68I	TCGCGA	1 cut(s)	291
BspANI	GGCC	8 cut(s)	65, 161, 939, 1257, 1268, 1548, 2210, 2745
BspCNI	CTCAG	6 cut(s)	360, 873, 1663, 1714, 1852, 2212
BspFNI	CGCG	5 cut(s)	39, 144, 291, 447, 675
BspHI	TCATGA	3 cut(s)	787, 1354, 2011
BspLI	GGNNCC	4 cut(s)	43, 1256, 1879, 2973
BspMAI	CTGCAG	1 cut(s)	1738
BspMI	ACCTGC	2 cut(s)	420, 2237
BspOI	GCTAGC	1 cut(s)	2933
BspPI	GGATC	7 cut(s)	1442, 2249, 2811, 2825, 2899, 2966, 2979
BspQI	GCTCTTC	1 cut(s)	1143
BspT104I	TTCGAA	1 cut(s)	770
BspT107I	GGYRCC	2 cut(s)	41, 1877
BspTI	CTTAAG	1 cut(s)	2387
BspTNI	GGTCTC	3 cut(s)	146, 525, 1940
BsrDI	GCAATG	1 cut(s)	2197
BsrFI	RCCGGY	3 cut(s)	235, 2049, 2210
BsrGI	TGTACA	1 cut(s)	1987
BsrI	ACTGG	5 cut(s)	1952, 2024, 2401, 2640, 3028
BssAI	RCCGGY	3 cut(s)	235, 2049, 2210
BssECI	CCNNGG	6 cut(s)	15, 37, 156, 445, 645, 1881
BssNI	GRCGYC	2 cut(s)	12, 2400
BssT1I	CCWWGG	2 cut(s)	156, 645
Bst2UI	CCWGG	6 cut(s)	17, 336, 861, 1427, 1550, 1773
Bst6I	CTCTTC	8 cut(s)	52, 54, 141, 753, 767, 1143, 1548, 1575
BstACI	GRCGYC	2 cut(s)	12, 2400
BstAFI	CTTAAG	1 cut(s)	2387
BstAUI	TGTACA	1 cut(s)	1987
BstBI	TTCGAA	1 cut(s)	770
BstC8I	GCNNGC	5 cut(s)	32, 445, 1546, 2618, 2931
BstDEI	CTNAG	8 cut(s)	368, 881, 1609, 1671, 1701, 1839, 2220, 2415
BstDSI	CCRYGG	3 cut(s)	37, 445, 1881
BstENI	CCTNNNNNAGG	1 cut(s)	528
BstF5I	GGATG	9 cut(s)	895, 1201, 1234, 1498, 1564, 1813, 2029, 2629, 3010
BstFNI	CGCG	5 cut(s)	39, 144, 291, 447, 675
BstH2I	RGCGCY	2 cut(s)	88, 2272
BstHHI	GCGC	5 cut(s)	87, 144, 675, 2271, 2376
BstMCI	CGRYCG	1 cut(s)	451
BstMWI	GCNNNNNNNGC	9 cut(s)	31, 36, 91, 105, 141, 1636, 1696, 1711, 2546
BstNI	CCWGG	6 cut(s)	17, 336, 861, 1427, 1550, 1773
BstNSI	RCATGY	2 cut(s)	1504, 2491
BstPAI	GACNNNNGTC	1 cut(s)	2120
BstSCI	CCNGG	7 cut(s)	15, 334, 859, 1425, 1548, 1771, 2695
BstSFI	CTRYAG	3 cut(s)	1734, 2041, 2820
BstSLI	GKGCMC	1 cut(s)	834
BstUI	CGCG	5 cut(s)	39, 144, 291, 447, 675
BstV1I	GCAGC	4 cut(s)	144, 1769, 1807, 2077
BstV2I	GAAGAC	2 cut(s)	249, 1503
BstX2I	RGATCY	4 cut(s)	731, 2803, 2944, 2971
BstYI	RGATCY	4 cut(s)	731, 2803, 2944, 2971
BsuI	GTATCC	1 cut(s)	1412
BsuRI	GGCC	8 cut(s)	65, 161, 939, 1257, 1268, 1548, 2210, 2745
BtgI	CCRYGG	3 cut(s)	37, 445, 1881
BtgZI	GCGATG	2 cut(s)	11, 306
BtrI	CACGTC	1 cut(s)	247
BtsCI	GGATG	9 cut(s)	895, 1201, 1234, 1498, 1564, 1813, 2029, 2629, 3010
BtsI	GCAGTG	1 cut(s)	2085
BtsIMutI	CAGTG	7 cut(s)	877, 1725, 2085, 2272, 2432, 2633, 3021
BtuMI	TCGCGA	1 cut(s)	291
BveI	ACCTGC	2 cut(s)	420, 2237
Cac8I	GCNNGC	5 cut(s)	32, 445, 1546, 2618, 2931
CaiI	CAGNNNCTG	3 cut(s)	1708, 1763, 2806
CciI	TCATGA	3 cut(s)	787, 1354, 2011
CfoI	GCGC	5 cut(s)	87, 144, 675, 2271, 2376
Cfr10I	RCCGGY	3 cut(s)	235, 2049, 2210
Cfr13I	GGNCC	4 cut(s)	1255, 1266, 1429, 2744
Cfr42I	CCGCGG	2 cut(s)	40, 448
CseI	GACGC	3 cut(s)	20, 1643, 2389
CsiI	ACCWGGT	1 cut(s)	1771
Csp6I	GTAC	6 cut(s)	637, 1460, 1899, 1988, 2038, 2665
CspAI	ACCGGT	1 cut(s)	2049
CspCI	CAANNNNNGTGG	4 cut(s)	384, 419, 1870, 1905
CviQI	GTAC	6 cut(s)	637, 1460, 1899, 1988, 2038, 2665
DdeI	CTNAG	8 cut(s)	368, 881, 1609, 1671, 1701, 1839, 2220, 2415
DraI	TTTAAA	2 cut(s)	2445, 2941
EaeI	YGGCCR	1 cut(s)	1546
Eam1104I	CTCTTC	8 cut(s)	52, 54, 141, 753, 767, 1143, 1548, 1575
EarI	CTCTTC	8 cut(s)	52, 54, 141, 753, 767, 1143, 1548, 1575
Eco130I	CCWWGG	2 cut(s)	156, 645
Eco31I	GGTCTC	3 cut(s)	146, 525, 1940
Eco32I	GATATC	3 cut(s)	1363, 1936, 2641
Eco47I	GGWCC	1 cut(s)	1429
Eco57I	CTGAAG	2 cut(s)	2181, 2456
EcoNI	CCTNNNNNAGG	1 cut(s)	528
EcoO109I	RGGNCCY	1 cut(s)	2744
EcoRII	CCWGG	6 cut(s)	15, 334, 859, 1425, 1548, 1771
EcoRV	GATATC	3 cut(s)	1363, 1936, 2641
EcoT14I	CCWWGG	2 cut(s)	156, 645
ErhI	CCWWGG	2 cut(s)	156, 645
FalI	AAGNNNNNCTT	6 cut(s)	539, 571, 750, 782, 2093, 2125
FaqI	GGGAC	8 cut(s)	420, 644, 809, 1034, 1181, 1196, 1229, 1250
FauI	CCCGC	2 cut(s)	452, 749
FauNDI	CATATG	1 cut(s)	2340
FbaI	TGATCA	2 cut(s)	2626, 2797
FblI	GTMKAC	3 cut(s)	168, 1657, 2725
Fnu4HI	GCNGC	7 cut(s)	109, 133, 194, 1758, 1821, 2091, 2584
FokI	GGATG	9 cut(s)	902, 1208, 1241, 1505, 1571, 1820, 2036, 2616, 2997
Fsp4HI	GCNGC	7 cut(s)	109, 133, 194, 1758, 1821, 2091, 2584
FspBI	CTAG	6 cut(s)	489, 498, 528, 1778, 1863, 2930
GlaI	GCGC	5 cut(s)	86, 143, 674, 2270, 2375
GluI	GCNGC	7 cut(s)	109, 133, 194, 1758, 1821, 2091, 2584
GsuI	CTGGAG	4 cut(s)	357, 1730, 1766, 1887
HaeII	RGCGCY	2 cut(s)	88, 2272
HaeIII	GGCC	8 cut(s)	65, 161, 939, 1257, 1268, 1548, 2210, 2745
HapII	CCGG	5 cut(s)	216, 236, 2050, 2211, 2697
HgaI	GACGC	3 cut(s)	20, 1643, 2389
HhaI	GCGC	5 cut(s)	87, 144, 675, 2271, 2376
Hin1I	GRCGYC	2 cut(s)	12, 2400
Hin6I	GCGC	5 cut(s)	85, 142, 673, 2269, 2374
HinP1I	GCGC	5 cut(s)	85, 142, 673, 2269, 2374
HincII	GTYRAC	3 cut(s)	169, 2226, 2988
HindII	GTYRAC	3 cut(s)	169, 2226, 2988
HindIII	AAGCTT	1 cut(s)	3000
HpaII	CCGG	5 cut(s)	216, 236, 2050, 2211, 2697
HphI	GGTGA	9 cut(s)	113, 332, 648, 689, 1827, 1955, 2165, 2872, 2924
Hpy166II	GTNNAC	9 cut(s)	169, 656, 832, 1658, 1988, 2156, 2226, 2726, 2988
Hpy188I	TCNGA	7 cut(s)	52, 369, 471, 841, 882, 2405, 2808
Hpy8I	GTNNAC	9 cut(s)	169, 656, 832, 1658, 1988, 2156, 2226, 2726, 2988
Hpy99I	CGWCG	6 cut(s)	14, 71, 167, 170, 173, 272
HpyCH4IV	ACGT	2 cut(s)	246, 2722
HpyCH4V	TGCA	9 cut(s)	415, 832, 1684, 1736, 1823, 2030, 2129, 2432, 2921
HpyF10VI	GCNNNNNNNGC	9 cut(s)	31, 36, 91, 105, 141, 1636, 1696, 1711, 2546
HpyF3I	CTNAG	8 cut(s)	368, 881, 1609, 1671, 1701, 1839, 2220, 2415
HpySE526I	ACGT	2 cut(s)	246, 2722
Hsp92I	GRCGYC	2 cut(s)	12, 2400
HspAI	GCGC	5 cut(s)	85, 142, 673, 2269, 2374
Ksp22I	TGATCA	2 cut(s)	2626, 2797
KspI	CCGCGG	2 cut(s)	40, 448
LguI	GCTCTTC	1 cut(s)	1143
Lsp1109I	GCAGC	4 cut(s)	144, 1769, 1807, 2077
LweI	GCATC	7 cut(s)	34, 430, 1360, 2017, 2059, 2751, 2916
MabI	ACCWGGT	1 cut(s)	1771
MaeI	CTAG	6 cut(s)	489, 498, 528, 1778, 1863, 2930
MaeII	ACGT	2 cut(s)	246, 2722
MflI	RGATCY	4 cut(s)	731, 2803, 2944, 2971
MhlI	GDGCHC	2 cut(s)	834, 2599
MlsI	TGGCCA	1 cut(s)	1548
MluNI	TGGCCA	1 cut(s)	1548
MmeI	TCCRAC	1 cut(s)	1936
Mox20I	TGGCCA	1 cut(s)	1548
MroXI	GAANNNNTTC	4 cut(s)	1533, 1870, 2334, 2607
MscI	TGGCCA	1 cut(s)	1548
MseI	TTAA	7 cut(s)	2175, 2204, 2360, 2388, 2444, 2868, 2940
MslI	CAYNNNNRTG	5 cut(s)	39, 414, 420, 786, 803
Msp20I	TGGCCA	1 cut(s)	1548
MspA1I	CMGCKG	2 cut(s)	39, 447
MspCI	CTTAAG	1 cut(s)	2387
MspI	CCGG	5 cut(s)	216, 236, 2050, 2211, 2697
MspR9I	CCNGG	7 cut(s)	17, 336, 861, 1427, 1550, 1773, 2697
Mva1269I	GAATGC	3 cut(s)	1533, 1872, 2923
MvaI	CCWGG	6 cut(s)	17, 336, 861, 1427, 1550, 1773
MvnI	CGCG	5 cut(s)	39, 144, 291, 447, 675
MwoI	GCNNNNNNNGC	9 cut(s)	31, 36, 91, 105, 141, 1636, 1696, 1711, 2546
NciI	CCSGG	1 cut(s)	2697
NdeI	CATATG	1 cut(s)	2340
NheI	GCTAGC	1 cut(s)	2929
NlaIV	GGNNCC	4 cut(s)	43, 1256, 1879, 2973
NmeAIII	GCCGAG	1 cut(s)	1283
NmuCI	GTSAC	7 cut(s)	119, 125, 677, 1061, 1632, 1674, 2249
NruI	TCGCGA	1 cut(s)	291
NspI	RCATGY	2 cut(s)	1504, 2491
NspV	TTCGAA	1 cut(s)	770
OliI	CACNNNNGTG	2 cut(s)	39, 803
PagI	TCATGA	3 cut(s)	787, 1354, 2011
PaqCI	CACCTGC	1 cut(s)	420
PciI	ACATGT	1 cut(s)	1500
PciSI	GCTCTTC	1 cut(s)	1143
PcsI	WCGNNNNNNNCGW	3 cut(s)	63, 174, 288
PctI	GAATGC	3 cut(s)	1533, 1872, 2923
PdmI	GAANNNNTTC	4 cut(s)	1533, 1870, 2334, 2607
PfeI	GAWTC	7 cut(s)	212, 364, 494, 1014, 1572, 2330, 2344
PflFI	GACNNNGTC	2 cut(s)	173, 1220
PfoI	TCCNGGA	1 cut(s)	334
PinAI	ACCGGT	1 cut(s)	2049
PkrI	GCNGC	7 cut(s)	110, 134, 195, 1759, 1822, 2092, 2585
PscI	ACATGT	1 cut(s)	1500
PshAI	GACNNNNGTC	1 cut(s)	2120
Psp6I	CCWGG	6 cut(s)	15, 334, 859, 1425, 1548, 1771
PspGI	CCWGG	6 cut(s)	15, 334, 859, 1425, 1548, 1771
PspN4I	GGNNCC	4 cut(s)	43, 1256, 1879, 2973
PspPI	GGNCC	4 cut(s)	1255, 1266, 1429, 2744
PstI	CTGCAG	1 cut(s)	1738
PstNI	CAGNNNCTG	3 cut(s)	1708, 1763, 2806
PsuI	RGATCY	4 cut(s)	731, 2803, 2944, 2971
PsyI	GACNNNGTC	2 cut(s)	173, 1220
RruI	TCGCGA	1 cut(s)	291
RsaI	GTAC	6 cut(s)	638, 1461, 1900, 1989, 2039, 2666
RsaNI	GTAC	6 cut(s)	637, 1460, 1899, 1988, 2038, 2665
RseI	CAYNNNNRTG	5 cut(s)	39, 414, 420, 786, 803
SacII	CCGCGG	2 cut(s)	40, 448
SalI	GTCGAC	1 cut(s)	167
SapI	GCTCTTC	1 cut(s)	1143
SaqAI	TTAA	7 cut(s)	2175, 2204, 2360, 2388, 2444, 2868, 2940
SatI	GCNGC	7 cut(s)	109, 133, 194, 1758, 1821, 2091, 2584
Sau96I	GGNCC	4 cut(s)	1255, 1266, 1429, 2744
ScrFI	CCNGG	7 cut(s)	17, 336, 861, 1427, 1550, 1773, 2697
SduI	GDGCHC	2 cut(s)	834, 2599
SexAI	ACCWGGT	1 cut(s)	1771
SfaNI	GCATC	7 cut(s)	34, 430, 1360, 2017, 2059, 2751, 2916
SfcI	CTRYAG	3 cut(s)	1734, 2041, 2820
Sfr303I	CCGCGG	2 cut(s)	40, 448
SfuI	TTCGAA	1 cut(s)	770
SgrBI	CCGCGG	2 cut(s)	40, 448
SgrDI	CGTCGACG	1 cut(s)	167
SinI	GGWCC	1 cut(s)	1429
SmiMI	CAYNNNNRTG	5 cut(s)	39, 414, 420, 786, 803
SmlI	CTYRAG	1 cut(s)	2387
SmoI	CTYRAG	1 cut(s)	2387
SspI	AATATT	2 cut(s)	910, 2380
SspMI	CTAG	6 cut(s)	489, 498, 528, 1778, 1863, 2930
StyD4I	CCNGG	7 cut(s)	15, 334, 859, 1425, 1548, 1771, 2695
StyI	CCWWGG	2 cut(s)	156, 645
TaiI	ACGT	2 cut(s)	249, 2725
TaqII	GACCGA	2 cut(s)	1296, 1446
TatI	WGTACW	2 cut(s)	636, 1987
TauI	GCSGC	3 cut(s)	111, 196, 2586
TfiI	GAWTC	7 cut(s)	212, 364, 494, 1014, 1572, 2330, 2344
Tru1I	TTAA	7 cut(s)	2175, 2204, 2360, 2388, 2444, 2868, 2940
Tru9I	TTAA	7 cut(s)	2175, 2204, 2360, 2388, 2444, 2868, 2940
TscAI	CASTG	7 cut(s)	877, 1725, 2092, 2279, 2439, 2640, 3028
TseFI	GTSAC	7 cut(s)	119, 125, 677, 1061, 1632, 1674, 2249
TseI	GCWGC	4 cut(s)	132, 1757, 1820, 2090
Tsp45I	GTSAC	7 cut(s)	119, 125, 677, 1061, 1632, 1674, 2249
TspGWI	ACGGA	3 cut(s)	1045, 2114, 2200
TspRI	CASTG	7 cut(s)	877, 1725, 2092, 2279, 2439, 2640, 3028
Tth111I	GACNNNGTC	2 cut(s)	173, 1220
Vha464I	CTTAAG	1 cut(s)	2387
VneI	GTGCAC	1 cut(s)	830
VpaK11BI	GGWCC	1 cut(s)	1429
XagI	CCTNNNNNAGG	1 cut(s)	528
XapI	RAATTY	2 cut(s)	261, 2293
XbaI	TCTAGA	3 cut(s)	527, 1777, 1862
XceI	RCATGY	2 cut(s)	1504, 2491
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2640
XmiI	GTMKAC	3 cut(s)	168, 1657, 2725
XmnI	GAANNNNTTC	4 cut(s)	1533, 1870, 2334, 2607
XspI	CTAG	6 cut(s)	489, 498, 528, 1778, 1863, 2930

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.