Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0002761

Physical Location & Seq

Genomic Coordinates Forward (+)

57353615 .. 57361623

Gene Structure 8009 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00376710.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3072 bp

ATGTCCGGCGACGACCACGATTCGCGTCGCAAGCACCGCCGCGATCATTCCGACGACGACGCCGAGAAGTCCTCCAAGCGCCACAAGCACCGTCACCACCACCGCCACCACCGTCACCGCCACAGCAGCAAGAAGCGCGAAGAAGAGAGCAAGGCGGCCGCGGGTGAGGATATCGAAGCTCCTCCTCCGCCGCCTGTTATGTTGTCTCCTTCCAATGGTCCGCCTCCTCGGCCGGCCGAGGACGTCGAGGAAGGTGAAATTCTTGAGGACGGTGCCGGTGGTCGCGACGACGGCGTTTTGGAACCGAAAGCGGACTCTGGTGCCGAGTCTGGTGAAATTGAAGCTCCTGGAGTTCGCGAACGTTCTGATAAGCGAAATCTGGGACTTTATAGTGACAGTACCATGGATAAGAGCTCAAAGACCTTGGGCAAAGAACTTCGTAGTGATGATGATCCCAGGAGTCCTATTGGGGGTAAGTCTCATTGTGAGGCCGGCTTGCCTCAAAATTCTAGTGCTGAGCCTCGTGAAGAATCAGTTTCCAGAGTTGACCTTGAGGATCATGCCAATGGGAATTTAGAGAAGAGGTGGCACCTGGAATCTGGGTCCCCTTCTAAAGGAGGTGGTCATCGAAAGAAATACTATAGTGAAAATGTGGATGATGATAGAAGCAAATTAGCTAATAAAGATAAGTCACCCTATAGAAGTAGTGGTGACAGGTATGGTGAAGTGTTGCGTGGCAGGAGCAGATCAGGGTCACATGATCATGTGAGAGAGAGATCTCGTTCCTGTAGTATAGTTGACGAGGAGGCACCATCTAGAAGAAGATCTCATTCTCGCAGTATGTTAGAAGAGGAAGCTCTTTCTAAAAGGAGACGTTATCATGATCGAGAAATCTCAGTATATGCCGAGAAAAGCAAGGCCGCACACAACTCTGATGATGAAGGTATGGTGAGGGATGATGAAAGGGAACACAGTATTAGTTATAGCAGATACTCTGGATTAGATCGCAGTCGAGAGAGAGAGAGGAGTAGGGAGAGGGAGGTGGACAGGGTTCGGAGAAGGGAGAAAGAACTAGAGAGGAACAGGGGCAGGGATCGGAGAAGGGAGAAAGAAAGAGAAAGGAGTAGTGACAGGGATTTGTTTAGGGACAGGAAAAGGGGAATTGGACTTGGTGATAGCACTGAAAGGGTGGTTGATACGGAAAGTAGAAGGGAAGAGCGACATAGAAGCAGGGACAGGACAAGGGAGGATGATGTTGATAGGAGCAGGGGCAGGGGCAAGCCTGCTGACAGAGAGAGAGATAGAGATATGGAGAGGGATCGGGATAGGGATAGGGACAGAGTAACAGACCGGAAATGGGAGAGGCGGGATGATAGATATATGGACAAGGGTCGAGATATGGAGAGGGAGAGAAATGAAACTGTAAGCGGATATGGAACTCGAGACAAGCATAATCATTCTGGTCAATCAAGGTATGAAGAAAAAGAATATAACCAGGATGGTTCAACAAAATCTGATCAAGCAGAAGTTCAGCTAGAAAGAGATGAAGATGAACAAGGAGATGATGAAGAGATGGTCAGTTTGCAAGTTGCTGATCAGGAAGAGGAAGATCTTAACAGAATTAAGGAGGAAAGTAGGAGGAGAAAGCAAGCAATCTTGGAAAAATATAAAAGTCAGCAGTCACAGTCCCAGCAAAAGACCGACGTACAAATTGAGTCACAGTTACAGCAACCCACTCAATTAGAGGGTCAAAAAGATCATCTGGATCAATCAACAGGGGTTGCCAAATCTAGAGCAGAAGTTGATGGTGCTAGGAGTTCTGGAGGAGTATATATGGCTGAAGCATTTTCTACTACAGCTCGTCAGAATGGTGCCCCTGGGCTTGGAGAGGGTACTCCAAAGAGTGAAAGCTCTGGTGGCTCAGAACCCAAAAAAGAAAGAGCTGATGGTATTTTATGCGATGATATCTTTGGTGAGACCCCAACTGGAGTTCAAAAATTGGACAAAGGAGATGCTGTACGAATTGAAAGGAGTGGTCTCCGTGACAACTGGGATGATGCAGAAGGGTACTACAGCTACCGGTTTGGTGAGTTACTTGATGGTCGATATGAGATTACCGCTGCTCATGGAAAAGGCGTGTTTTCAACTGTTGTTCGTGCAAAGAACCTTAAGGTTGGTAATGGTGAACCAGAAGAAGTGGCAATTAAGATTATTCGTAGCAACGATACTATGTACAAGGCTGGTATGACTGAGTTGGCCATATTGAAGAAATTAGTAGGTCAAGATCCAGACAATAAGCGCTATTGTGTTCGTTTTCTATCAAGTTTCAAGTATCGAAATCATCTTTGTTTAGTTTTTGAATCTCTGCACATGAATCTTCGTGAGCTTTTAAAGAAGTTTGGACGCAATATTGGTCTGAATCTAACTGCGGTGAGAGTATATGCTAAGCAACTCTTGATTGCATTGAAGCATCTCAAAAACTGTGGTGTTCTTCATTGTGATATAAAGCCAGATAACATGCTGGTAAATGAAGCAAAAAACGTTCTGAAGCTTTGTGATTTTGGTAACGCCATGTTTGCTGGCAAGAATGAAGTTACACCGTACCTTGTGAGCCGATTTTATCGGGCCCCTGAAATAATTCTGGGCTTGCCTTATGATCATCCACTGGATATCTGGTCTGTTGGTTGCTGTTTGTTTGAACTTTATACTGGAAAAGTTCTTTTTCCTGGTGCTACGAACAATGATATGCTACGTCTTCACATGGAATTGAAAGGCCCTTTCCCTAAGAAGATGCTTCGGAAGGCAACATTTACTTGTCAAAATTTCGATCAGGATCTGAACTTCCATGCCACAGAAGATGATCCTGTTACAAAAAAGGTTATAAAACGCGTGATTCTCAACATAAAGCCAAAAGATATCGGATCAATGATCGCAGGTTCTCCTGGCGAGGATCCAGATCCCAAGATATTAGCTGATTTTAAAGATCTTTTAGATAAAATGTTTGTGCTGGATCCAGACAAGAGGTTGACAGTATCACAAGGTTTGAATCATCCATTCATCACTGGCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016310 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase TBPIP

Protein Analysis

1023

Amino Acids

117.05

Weight (kDa)

6.97

Isoelectric Point (pI)

58.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	704 - 1020	3.9e-47	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	709 - 920	4.2e-19	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002761)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13350 AT1G13350 AT1G13350 AT3G25840 AT3G25840 AT3G53640
fragaria_vesca	FvH4_4g27640 FvH4_4g27640 FvH4_4g27640 FvH4_4g27640 FvH4_4g27640 FvH4_4g27640 FvH4_4g27640
malus_domestica	MD13G1043800.v1.1 MD16G1044600.v1.1
prunus_persica	Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1
pyrus_communis	pycom16g03900
rosa_chinensis	RchiOBHm_Chr4g0435481
rosa_laevigata	RLG00000006589
rosa_multiflora	Rmu_sc0001940.1_g000027
rosa_roxburghii	Rroxscaffold_5G00376710
rosa_rugosa	Rorug04G0285400 Rorug04G0285500
rosa_samantha	Rh4AG341100 Rh4BG349300 Rh4CG364000 Rh4DG343600
rosa_wichuraiana	Rw4G029760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2882
AatII	GACGTC	1 cut(s)	246
Acc36I	ACCTGC	1 cut(s)	2924
AccB1I	GGYRCC	5 cut(s)	272, 320, 588, 808, 1871
AccII	CGCG	7 cut(s)	25, 42, 138, 161, 285, 357, 2889
AclI	AACGTT	2 cut(s)	361, 2541
AcoI	YGGCCR	4 cut(s)	156, 230, 234, 2256
AcsI	RAATTY	4 cut(s)	258, 505, 571, 2821
AcuI	CTGAAG	2 cut(s)	1860, 2567
AcyI	GRCGYC	2 cut(s)	60, 243
AfaI	GTAC	7 cut(s)	400, 1707, 1894, 2019, 2069, 2234, 2603
AfeI	AGCGCT	1 cut(s)	2300
AfiI	CCNNNNNNNGG	5 cut(s)	215, 470, 614, 1357, 1883
AflII	CTTAAG	1 cut(s)	2168
AflIII	ACRYGT	1 cut(s)	2887
AgeI	ACCGGT	1 cut(s)	2079
AjnI	CCWGG	7 cut(s)	346, 455, 591, 1494, 1876, 2725, 2941
Alw21I	GWGCWC	1 cut(s)	416
Alw26I	GTCTC	6 cut(s)	210, 483, 865, 1437, 1970, 2042
AlwNI	CAGNNNCTG	1 cut(s)	2836
Ama87I	CYCGRG	1 cut(s)	1440
Aor51HI	AGCGCT	1 cut(s)	2300
AoxI	GGCC	8 cut(s)	156, 230, 234, 489, 918, 2256, 2625, 2773
ApaI	GGGCCC	1 cut(s)	2629
ApeKI	GCWGC	2 cut(s)	126, 2120
ApoI	RAATTY	4 cut(s)	258, 505, 571, 2821
AsiGI	ACCGGT	1 cut(s)	2079
Asp700I	GAANNNNTTC	1 cut(s)	2637
AspLEI	GCGC	3 cut(s)	81, 138, 2301
AspS9I	GGNCC	5 cut(s)	218, 603, 2625, 2626, 2774
AvaI	CYCGRG	1 cut(s)	1440
AvaII	GGWCC	2 cut(s)	218, 603
BaeGI	GKGCMC	2 cut(s)	1876, 2629
BalI	TGGCCA	1 cut(s)	2258
BamHI	GGATCC	2 cut(s)	2950, 3010
BanI	GGYRCC	5 cut(s)	272, 320, 588, 808, 1871
BanII	GRGCYC	2 cut(s)	416, 2629
BauI	CACGAG	1 cut(s)	522
BbsI	GAAGAC	1 cut(s)	2747
Bbv12I	GWGCWC	1 cut(s)	416
BbvI	GCAGC	2 cut(s)	138, 2107
BccI	CCATC	6 cut(s)	820, 1493, 1567, 1799, 1940, 2093
BceAI	ACGGC	1 cut(s)	307
BciT130I	CCWGG	7 cut(s)	348, 457, 593, 1496, 1878, 2727, 2943
BclI	TGATCA	4 cut(s)	760, 1516, 1594, 2656
BcoDI	GTCTC	6 cut(s)	210, 483, 865, 1437, 1970, 2042
BfaI	CTAG	6 cut(s)	510, 816, 1073, 1535, 1791, 1812
BfmI	CTRYAG	5 cut(s)	640, 697, 787, 1854, 2071
BfoI	RGCGCY	2 cut(s)	82, 2302
BfrI	CTTAAG	1 cut(s)	2168
BfuAI	ACCTGC	1 cut(s)	2924
BglI	GCCNNNNNGGC	1 cut(s)	229
BglII	AGATCT	4 cut(s)	776, 824, 1609, 2983
BisI	GCNGC	7 cut(s)	40, 127, 156, 159, 191, 921, 2121
BlpI	GCTNAGC	2 cut(s)	516, 2445
BlsI	GCNGC	7 cut(s)	41, 128, 157, 160, 192, 922, 2122
Bme1390I	CCNGG	7 cut(s)	348, 457, 593, 1496, 1878, 2727, 2943
Bme18I	GGWCC	2 cut(s)	218, 603
BmeT110I	CYCGRG	1 cut(s)	1440
BmgT120I	GGNCC	5 cut(s)	218, 603, 2625, 2626, 2774
BmrFI	CCNGG	7 cut(s)	348, 457, 593, 1496, 1878, 2727, 2943
BmrI	ACTGGG	1 cut(s)	2059
BmsI	GCATC	4 cut(s)	2002, 2047, 2479, 2781
BmuI	ACTGGG	1 cut(s)	2059
BoxI	GACNNNNGTC	1 cut(s)	1389
BpiI	GAAGAC	1 cut(s)	2747
BplI	GAGNNNNNCTC	2 cut(s)	56, 88
BpmI	CTGGAG	3 cut(s)	369, 1842, 2007
Bpu1102I	GCTNAGC	2 cut(s)	516, 2445
BpuEI	CTTGAG	2 cut(s)	284, 572
BsaBI	GATNNNNATC	3 cut(s)	450, 2832, 2955
BsaHI	GRCGYC	2 cut(s)	60, 243
BsaI	GGTCTC	2 cut(s)	1970, 2042
BsaJI	CCNNGG	8 cut(s)	159, 227, 237, 402, 423, 455, 1876, 1877
BsaWI	WCCGGW	2 cut(s)	1350, 2079
BsaXI	ACNNNNNCTCC	2 cut(s)	2001, 2031
Bsc4I	CCNNNNNNNGG	5 cut(s)	215, 470, 614, 1357, 1883
Bse118I	RCCGGY	4 cut(s)	232, 275, 491, 2079
Bse1I	ACTGG	5 cut(s)	1990, 2054, 2670, 2713, 3067
Bse8I	GATNNNNATC	3 cut(s)	450, 2832, 2955
BseBI	CCWGG	7 cut(s)	348, 457, 593, 1496, 1878, 2727, 2943
BseDI	CCNNGG	8 cut(s)	159, 227, 237, 402, 423, 455, 1876, 1877
BseGI	GGATG	8 cut(s)	661, 961, 1255, 1375, 1504, 2059, 2659, 3049
BseJI	GATNNNNATC	3 cut(s)	450, 2832, 2955
BseLI	CCNNNNNNNGG	5 cut(s)	215, 470, 614, 1357, 1883
BseMII	CTCAG	4 cut(s)	507, 909, 1935, 2241
BseNI	ACTGG	5 cut(s)	1990, 2054, 2670, 2713, 3067
BseRI	GAGGAG	7 cut(s)	171, 174, 216, 818, 1039, 1654, 1839
BseSI	GKGCMC	2 cut(s)	1876, 2629
BseX3I	CGGCCG	3 cut(s)	156, 230, 234
BseXI	GCAGC	2 cut(s)	138, 2107
BseYI	CCCAGC	1 cut(s)	1689
BsgI	GTGCAG	1 cut(s)	2351
Bsh1236I	CGCG	7 cut(s)	25, 42, 138, 161, 285, 357, 2889
Bsh1285I	CGRYCG	3 cut(s)	159, 233, 237
BshFI	GGCC	8 cut(s)	158, 232, 236, 491, 920, 2258, 2627, 2775
BshNI	GGYRCC	5 cut(s)	272, 320, 588, 808, 1871
BshTI	ACCGGT	1 cut(s)	2079
BsiEI	CGRYCG	3 cut(s)	159, 233, 237
BsiHKAI	GWGCWC	1 cut(s)	416
BsiHKCI	CYCGRG	1 cut(s)	1440
BsiSI	CCGG	6 cut(s)	6, 233, 276, 492, 1351, 2080
BslFI	GGGAC	6 cut(s)	396, 589, 1160, 1247, 1349, 1672
BslI	CCNNNNNNNGG	5 cut(s)	215, 470, 614, 1357, 1883
BsmAI	GTCTC	6 cut(s)	210, 483, 865, 1437, 1970, 2042
BsmBI	CGTCTC	1 cut(s)	865
BsmFI	GGGAC	6 cut(s)	396, 589, 1160, 1247, 1349, 1672
BsnI	GGCC	8 cut(s)	158, 232, 236, 491, 920, 2258, 2627, 2775
Bso31I	GGTCTC	2 cut(s)	1970, 2042
BsoBI	CYCGRG	1 cut(s)	1440
Bsp120I	GGGCCC	1 cut(s)	2625
Bsp1286I	GDGCHC	3 cut(s)	416, 1876, 2629
Bsp1407I	TGTACA	1 cut(s)	2232
Bsp1720I	GCTNAGC	2 cut(s)	516, 2445
Bsp19I	CCATGG	1 cut(s)	402
Bsp68I	TCGCGA	2 cut(s)	285, 357
BspANI	GGCC	8 cut(s)	158, 232, 236, 491, 920, 2258, 2627, 2775
BspCNI	CTCAG	4 cut(s)	508, 908, 1934, 2242
BspFNI	CGCG	7 cut(s)	25, 42, 138, 161, 285, 357, 2889
BspHI	TCATGA	1 cut(s)	880
BspMI	ACCTGC	1 cut(s)	2924
BspQI	GCTCTTC	1 cut(s)	1209
BspT107I	GGYRCC	5 cut(s)	272, 320, 588, 808, 1871
BspTI	CTTAAG	1 cut(s)	2168
BspTNI	GGTCTC	2 cut(s)	1970, 2042
BsrFI	RCCGGY	4 cut(s)	232, 275, 491, 2079
BsrGI	TGTACA	1 cut(s)	2232
BsrI	ACTGG	5 cut(s)	1990, 2054, 2670, 2713, 3067
BssAI	RCCGGY	4 cut(s)	232, 275, 491, 2079
BssECI	CCNNGG	8 cut(s)	159, 227, 237, 402, 423, 455, 1876, 1877
BssNI	GRCGYC	2 cut(s)	60, 243
BssSI	CACGAG	1 cut(s)	522
BssT1I	CCWWGG	2 cut(s)	402, 423
Bst2BI	CACGAG	1 cut(s)	522
Bst2UI	CCWGG	7 cut(s)	348, 457, 593, 1496, 1878, 2727, 2943
Bst6I	CTCTTC	6 cut(s)	138, 575, 843, 1209, 1563, 1596
BstACI	GRCGYC	2 cut(s)	60, 243
BstAFI	CTTAAG	1 cut(s)	2168
BstAUI	TGTACA	1 cut(s)	2232
BstC8I	GCNNGC	9 cut(s)	32, 234, 493, 497, 1280, 1284, 1650, 2581, 2648
BstDEI	CTNAG	6 cut(s)	516, 895, 1921, 2250, 2445, 2784
BstDSI	CCRYGG	2 cut(s)	159, 402
BstENI	CCTNNNNNAGG	1 cut(s)	612
BstF5I	GGATG	8 cut(s)	661, 961, 1255, 1375, 1504, 2059, 2659, 3049
BstFNI	CGCG	7 cut(s)	25, 42, 138, 161, 285, 357, 2889
BstH2I	RGCGCY	2 cut(s)	82, 2302
BstHHI	GCGC	3 cut(s)	81, 138, 2301
BstMAI	GTCTC	6 cut(s)	210, 483, 865, 1437, 1970, 2042
BstMCI	CGRYCG	3 cut(s)	159, 233, 237
BstMWI	GCNNNNNNNGC	9 cut(s)	31, 36, 85, 126, 135, 229, 291, 1917, 2576
BstNI	CCWGG	7 cut(s)	348, 457, 593, 1496, 1878, 2727, 2943
BstNSI	RCATGY	1 cut(s)	2521
BstPAI	GACNNNNGTC	1 cut(s)	1389
BstSCI	CCNGG	7 cut(s)	346, 455, 591, 1494, 1876, 2725, 2941
BstSFI	CTRYAG	5 cut(s)	640, 697, 787, 1854, 2071
BstSLI	GKGCMC	2 cut(s)	1876, 2629
BstUI	CGCG	7 cut(s)	25, 42, 138, 161, 285, 357, 2889
BstV1I	GCAGC	2 cut(s)	138, 2107
BstV2I	GAAGAC	1 cut(s)	2747
BstX2I	RGATCY	9 cut(s)	776, 824, 1609, 2284, 2833, 2950, 2956, 2983, 3010
BstYI	RGATCY	9 cut(s)	776, 824, 1609, 2284, 2833, 2950, 2956, 2983, 3010
BstZI	CGGCCG	3 cut(s)	156, 230, 234
BsuRI	GGCC	8 cut(s)	158, 232, 236, 491, 920, 2258, 2627, 2775
BtgI	CCRYGG	2 cut(s)	159, 402
BtgZI	GCGATG	1 cut(s)	1974
BtsCI	GGATG	8 cut(s)	661, 961, 1255, 1375, 1504, 2059, 2659, 3049
BtsIMutI	CAGTG	3 cut(s)	1179, 2663, 3060
BtuMI	TCGCGA	2 cut(s)	285, 357
BveI	ACCTGC	1 cut(s)	2924
Cac8I	GCNNGC	9 cut(s)	32, 234, 493, 497, 1280, 1284, 1650, 2581, 2648
CaiI	CAGNNNCTG	1 cut(s)	2836
CciI	TCATGA	1 cut(s)	880
CciNI	GCGGCCGC	1 cut(s)	156
CfoI	GCGC	3 cut(s)	81, 138, 2301
Cfr10I	RCCGGY	4 cut(s)	232, 275, 491, 2079
Cfr13I	GGNCC	5 cut(s)	218, 603, 2625, 2626, 2774
Cfr42I	CCGCGG	1 cut(s)	162
CseI	GACGC	3 cut(s)	14, 68, 2412
Csp6I	GTAC	7 cut(s)	399, 1706, 1893, 2018, 2068, 2233, 2602
CspAI	ACCGGT	1 cut(s)	2079
CspCI	CAANNNNNGTGG	2 cut(s)	2464, 2499
CviQI	GTAC	7 cut(s)	399, 1706, 1893, 2018, 2068, 2233, 2602
DdeI	CTNAG	6 cut(s)	516, 895, 1921, 2250, 2445, 2784
DraI	TTTAAA	2 cut(s)	2391, 2980
EaeI	YGGCCR	4 cut(s)	156, 230, 234, 2256
EagI	CGGCCG	3 cut(s)	156, 230, 234
Eam1104I	CTCTTC	6 cut(s)	138, 575, 843, 1209, 1563, 1596
EarI	CTCTTC	6 cut(s)	138, 575, 843, 1209, 1563, 1596
EciI	GGCGGA	2 cut(s)	177, 210
Ecl136II	GAGCTC	1 cut(s)	414
EclXI	CGGCCG	3 cut(s)	156, 230, 234
Eco130I	CCWWGG	2 cut(s)	402, 423
Eco24I	GRGCYC	2 cut(s)	416, 2629
Eco31I	GGTCTC	2 cut(s)	1970, 2042
Eco32I	GATATC	4 cut(s)	172, 1966, 2671, 2917
Eco47I	GGWCC	2 cut(s)	218, 603
Eco47III	AGCGCT	1 cut(s)	2300
Eco52I	CGGCCG	3 cut(s)	156, 230, 234
Eco53kI	GAGCTC	1 cut(s)	414
Eco57I	CTGAAG	2 cut(s)	1860, 2567
Eco88I	CYCGRG	1 cut(s)	1440
EcoICRI	GAGCTC	1 cut(s)	414
EcoNI	CCTNNNNNAGG	1 cut(s)	612
EcoO109I	RGGNCCY	3 cut(s)	603, 2626, 2774
EcoRII	CCWGG	7 cut(s)	346, 455, 591, 1494, 1876, 2725, 2941
EcoRV	GATATC	4 cut(s)	172, 1966, 2671, 2917
EcoT14I	CCWWGG	2 cut(s)	402, 423
EcoT38I	GRGCYC	2 cut(s)	416, 2629
ErhI	CCWWGG	2 cut(s)	402, 423
Esp3I	CGTCTC	1 cut(s)	865
FalI	AAGNNNNNCTT	2 cut(s)	2438, 2470
FaqI	GGGAC	6 cut(s)	396, 589, 1160, 1247, 1349, 1672
FauI	CCCGC	2 cut(s)	154, 1359
FbaI	TGATCA	4 cut(s)	760, 1516, 1594, 2656
Fnu4HI	GCNGC	7 cut(s)	40, 127, 156, 159, 191, 921, 2121
FokI	GGATG	8 cut(s)	668, 968, 1262, 1382, 1511, 2066, 2646, 3036
FriOI	GRGCYC	2 cut(s)	416, 2629
FseI	GGCCGGCC	1 cut(s)	236
Fsp4HI	GCNGC	7 cut(s)	40, 127, 156, 159, 191, 921, 2121
FspBI	CTAG	6 cut(s)	510, 816, 1073, 1535, 1791, 1812
GlaI	GCGC	3 cut(s)	80, 137, 2300
GluI	GCNGC	7 cut(s)	40, 127, 156, 159, 191, 921, 2121
GsaI	CCCAGC	1 cut(s)	1693
GsuI	CTGGAG	3 cut(s)	369, 1842, 2007
HaeII	RGCGCY	2 cut(s)	82, 2302
HaeIII	GGCC	8 cut(s)	158, 232, 236, 491, 920, 2258, 2627, 2775
HapII	CCGG	6 cut(s)	6, 233, 276, 492, 1351, 2080
HgaI	GACGC	3 cut(s)	14, 68, 2412
HhaI	GCGC	3 cut(s)	81, 138, 2301
Hin1I	GRCGYC	2 cut(s)	60, 243
Hin6I	GCGC	3 cut(s)	79, 136, 2299
HinP1I	GCGC	3 cut(s)	79, 136, 2299
HincII	GTYRAC	3 cut(s)	547, 799, 3027
HindII	GTYRAC	3 cut(s)	547, 799, 3027
HindIII	AAGCTT	1 cut(s)	2549
HpaII	CCGG	6 cut(s)	6, 233, 276, 492, 1351, 2080
Hpy166II	GTNNAC	5 cut(s)	547, 799, 1045, 2186, 3027
Hpy8I	GTNNAC	5 cut(s)	547, 799, 1045, 2186, 3027
Hpy99I	CGWCG	9 cut(s)	14, 30, 56, 59, 62, 248, 290, 293, 1706
HpyAV	CCTTC	9 cut(s)	219, 245, 618, 935, 1053, 1095, 1203, 2057, 2794
HpyCH4IV	ACGT	6 cut(s)	243, 361, 874, 1704, 2541, 2752
HpyCH4V	TGCA	5 cut(s)	1585, 2060, 2159, 2368, 2462
HpyF10VI	GCNNNNNNNGC	9 cut(s)	31, 36, 85, 126, 135, 229, 291, 1917, 2576
HpyF3I	CTNAG	6 cut(s)	516, 895, 1921, 2250, 2445, 2784
HpySE526I	ACGT	6 cut(s)	243, 361, 874, 1704, 2541, 2752
Hsp92I	GRCGYC	2 cut(s)	60, 243
HspAI	GCGC	3 cut(s)	79, 136, 2299
KflI	GGGWCCC	1 cut(s)	603
KroI	GCCGGC	2 cut(s)	232, 491
KroNI	GCCGGC	2 cut(s)	234, 493
Ksp22I	TGATCA	4 cut(s)	760, 1516, 1594, 2656
KspI	CCGCGG	1 cut(s)	162
LguI	GCTCTTC	1 cut(s)	1209
LmnI	GCTCC	4 cut(s)	184, 349, 741, 1263
Lsp1109I	GCAGC	2 cut(s)	138, 2107
LweI	GCATC	4 cut(s)	2002, 2047, 2479, 2781
MaeI	CTAG	6 cut(s)	510, 816, 1073, 1535, 1791, 1812
MaeII	ACGT	6 cut(s)	243, 361, 874, 1704, 2541, 2752
MflI	RGATCY	9 cut(s)	776, 824, 1609, 2284, 2833, 2950, 2956, 2983, 3010
MhlI	GDGCHC	3 cut(s)	416, 1876, 2629
MlsI	TGGCCA	1 cut(s)	2258
MluI	ACGCGT	1 cut(s)	2887
MluNI	TGGCCA	1 cut(s)	2258
MlyI	GAGTC	4 cut(s)	308, 335, 469, 1724
MmeI	TCCRAC	1 cut(s)	75
Mox20I	TGGCCA	1 cut(s)	2258
MroNI	GCCGGC	2 cut(s)	232, 491
MroXI	GAANNNNTTC	1 cut(s)	2637
MscI	TGGCCA	1 cut(s)	2258
MseI	TTAA	6 cut(s)	1614, 1623, 2169, 2205, 2390, 2979
MslI	CAYNNNNRTG	2 cut(s)	564, 762
Msp20I	TGGCCA	1 cut(s)	2258
MspA1I	CMGCKG	2 cut(s)	161, 2120
MspCI	CTTAAG	1 cut(s)	2168
MspI	CCGG	6 cut(s)	6, 233, 276, 492, 1351, 2080
MspR9I	CCNGG	7 cut(s)	348, 457, 593, 1496, 1878, 2727, 2943
MvaI	CCWGG	7 cut(s)	348, 457, 593, 1496, 1878, 2727, 2943
MvnI	CGCG	7 cut(s)	25, 42, 138, 161, 285, 357, 2889
MwoI	GCNNNNNNNGC	9 cut(s)	31, 36, 85, 126, 135, 229, 291, 1917, 2576
NaeI	GCCGGC	2 cut(s)	234, 493
NcoI	CCATGG	1 cut(s)	402
NgoMIV	GCCGGC	2 cut(s)	232, 491
NmeAIII	GCCGAG	5 cut(s)	88, 208, 262, 349, 931
NotI	GCGGCCGC	1 cut(s)	156
NruI	TCGCGA	2 cut(s)	285, 357
NspI	RCATGY	1 cut(s)	2521
PaeR7I	CTCGAG	1 cut(s)	1440
PagI	TCATGA	1 cut(s)	880
PasI	CCCWGGG	1 cut(s)	1877
PciSI	GCTCTTC	1 cut(s)	1209
PcsI	WCGNNNNNNNCGW	1 cut(s)	60
PdiI	GCCGGC	2 cut(s)	234, 493
PdmI	GAANNNNTTC	1 cut(s)	2637
PfeI	GAWTC	8 cut(s)	20, 530, 596, 2360, 2374, 2419, 2893, 3046
PfoI	TCCNGGA	1 cut(s)	346
PinAI	ACCGGT	1 cut(s)	2079
PkrI	GCNGC	7 cut(s)	41, 128, 157, 160, 192, 922, 2122
PleI	GAGTC	4 cut(s)	308, 334, 468, 1723
PpsI	GAGTC	4 cut(s)	308, 334, 468, 1723
PpuMI	RGGWCCY	1 cut(s)	603
PshAI	GACNNNNGTC	1 cut(s)	1389
PsiI	TTATAA	1 cut(s)	2882
Psp124BI	GAGCTC	1 cut(s)	416
Psp1406I	AACGTT	2 cut(s)	361, 2541
Psp5II	RGGWCCY	1 cut(s)	603
Psp6I	CCWGG	7 cut(s)	346, 455, 591, 1494, 1876, 2725, 2941
PspFI	CCCAGC	1 cut(s)	1689
PspGI	CCWGG	7 cut(s)	346, 455, 591, 1494, 1876, 2725, 2941
PspOMI	GGGCCC	1 cut(s)	2625
PspPI	GGNCC	5 cut(s)	218, 603, 2625, 2626, 2774
PspPPI	RGGWCCY	1 cut(s)	603
PstNI	CAGNNNCTG	1 cut(s)	2836
PsuI	RGATCY	9 cut(s)	776, 824, 1609, 2284, 2833, 2950, 2956, 2983, 3010
RigI	GGCCGGCC	1 cut(s)	236
RruI	TCGCGA	2 cut(s)	285, 357
RsaI	GTAC	7 cut(s)	400, 1707, 1894, 2019, 2069, 2234, 2603
RsaNI	GTAC	7 cut(s)	399, 1706, 1893, 2018, 2068, 2233, 2602
RseI	CAYNNNNRTG	2 cut(s)	564, 762
SacI	GAGCTC	1 cut(s)	416
SacII	CCGCGG	1 cut(s)	162
SapI	GCTCTTC	1 cut(s)	1209
SaqAI	TTAA	6 cut(s)	1614, 1623, 2169, 2205, 2390, 2979
SatI	GCNGC	7 cut(s)	40, 127, 156, 159, 191, 921, 2121
Sau96I	GGNCC	5 cut(s)	218, 603, 2625, 2626, 2774
SchI	GAGTC	4 cut(s)	308, 335, 469, 1724
ScrFI	CCNGG	7 cut(s)	348, 457, 593, 1496, 1878, 2727, 2943
SduI	GDGCHC	3 cut(s)	416, 1876, 2629
SfaNI	GCATC	4 cut(s)	2002, 2047, 2479, 2781
SfcI	CTRYAG	5 cut(s)	640, 697, 787, 1854, 2071
Sfr274I	CTCGAG	1 cut(s)	1440
Sfr303I	CCGCGG	1 cut(s)	162
SgrBI	CCGCGG	1 cut(s)	162
SinI	GGWCC	2 cut(s)	218, 603
SlaI	CTCGAG	1 cut(s)	1440
SmiMI	CAYNNNNRTG	2 cut(s)	564, 762
SmlI	CTYRAG	4 cut(s)	263, 551, 1440, 2168
SmoI	CTYRAG	4 cut(s)	263, 551, 1440, 2168
SspI	AATATT	1 cut(s)	2410
SspMI	CTAG	6 cut(s)	510, 816, 1073, 1535, 1791, 1812
SstI	GAGCTC	1 cut(s)	416
StyD4I	CCNGG	7 cut(s)	346, 455, 591, 1494, 1876, 2725, 2941
StyI	CCWWGG	2 cut(s)	402, 423
TaiI	ACGT	6 cut(s)	246, 364, 877, 1707, 2544, 2755
TaqII	GACCGA	1 cut(s)	1715
TatI	WGTACW	1 cut(s)	2232
TauI	GCSGC	5 cut(s)	42, 158, 161, 193, 923
TfiI	GAWTC	8 cut(s)	20, 530, 596, 2360, 2374, 2419, 2893, 3046
Tru1I	TTAA	6 cut(s)	1614, 1623, 2169, 2205, 2390, 2979
Tru9I	TTAA	6 cut(s)	1614, 1623, 2169, 2205, 2390, 2979
TscAI	CASTG	3 cut(s)	1186, 2670, 3067
TseI	GCWGC	2 cut(s)	126, 2120
TspGWI	ACGGA	2 cut(s)	1214, 2030
TspRI	CASTG	3 cut(s)	1186, 2670, 3067
Vha464I	CTTAAG	1 cut(s)	2168
VpaK11BI	GGWCC	2 cut(s)	218, 603
XagI	CCTNNNNNAGG	1 cut(s)	612
XapI	RAATTY	4 cut(s)	258, 505, 571, 2821
XbaI	TCTAGA	2 cut(s)	815, 1790
XceI	RCATGY	1 cut(s)	2521
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2670
XhoI	CTCGAG	1 cut(s)	1440
XmnI	GAANNNNTTC	1 cut(s)	2637
XspI	CTAG	6 cut(s)	510, 816, 1073, 1535, 1791, 1812
ZraI	GACGTC	1 cut(s)	244

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.