Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0002761

Physical Location & Seq

Genomic Coordinates Reverse (-)

8742865 .. 8748851

Gene Structure 5987 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000006589

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3156 bp

ATGTCCGGCGACGACCACGATTCGCGTCGCAAGCACCGCCGCGATCATTCCGACGACGACGCCGAGAAGTCCTCCAAGCGCCACAAGCACCGTCACCACCACCGCCACCACCGTCACCGCCACAGCAGCAAGAAGCGCGAAGAAGAGAGCAAGGCGGCCGCGGGTGAGGATATCGAAGCTCCTCCTCCGCCGCCTGTTATGCTGTCGCCTTCCAATAGTCCGCCTCCTCGGCCGGCCGAGGACGTCGAGGAAGGTGAAATTCTTGAGGACGGTGCCGGTGGTCGCGACGACGGCGTTTTGGAACCGAAAGCGGACTCTGGTGCCGAGTCTGGTGAAATTGAAGCTCCTGGAGTTCGCGAACGTTCTGATAAGCGAAATCTGGGACTTTATAGTGACAGTACCATGGATAAGAGCTCAAAGACCTTGGGCAAAGAACTTCGTAGTGATGATGATCCCAGGAGTCCTATTGGGGGTAAGTCTCATAGTGAGGCCGGCTTGCCTCAAAATTCTAGTGCCGAGCCTCGTGAAGAATCAGTTTCCAGAGTTGACCTTGAGGATCATGCCAATGGGAATTTAGAGAAGAGGCGGCACCTGGAATCTGGGTCCCCTTCTAAAGGAGGTGGTCATCGAAAGAAATACTATAGTGAAAATGTGGATGATGATAGAAGCAAGTTAGCTAATAAAGAAAAGTCACCCTATAGAAGTAGTGGTGACAGGTATGGTGAAGTGTTGCGCGGCAGGAGCAGATCAGGGTCAGATGATCATGTGAGAGAGAGATCTCGTTCCCGTAGTATAGTTGAAGAGGAGGCACCATCTAGAAGAAGATCTCATTCTCGCAGTATGTTAGAAGAGGAAGCTCTTTCTAAAAGGAGACGTTATCATGATCGAGAAATCTCAGTATATGCTGAGAAAAGCAAGGCCGCACACAACTCTGATGATGAAGGTATGGTGAGGGATGATGAAAGGGAACACAGTATTAGTTATAGCAGATACTCTGGATTAGATCGCAGTCGAGAGAGAGAGAGGAGTAGGGAGAGGGAGGTGGACAGGGTTCGGAGAAGGGAGAAAGAACTAGAGAGGAACAGGGGCAGGGATCGGAGAAGGGAGAAAGAAAGAGAAAGGAGTAGTGACAGGGATTTGTTTAGGGACAGGAAAAGGGGAATTGGACGTGGTGATAGCACTGAAAGGGTGGTTGATACGGAAAGTAGAAGGGAAGAGCGACATAGAAGCAGGGACAGAGAGAACAGGGACAGGACAAGGGAGGATGATGTTGATAGGAGCAGGGGCAGGGGCAAGCCTGCTGACAGAGAGAGAGATAGAGATATGGAGAGGGATCGGGATCGGGATCGGGATAGGGATAGGGACAGAGCAACAGACCGGAAATGGGAGAGGCGGGATGATAGATATAGGGACAAGGGTCGAGATATGGAGAGGGAGAGAAATGAAACTGAAAGTGGATATGGAACTCGAGACAAGCATAATCATTCTGGTCAATCAAGGTATGAAGAAAAAGAATATAACCAGGATGGTTCAACAAAATCTGATCAAGCAGAATTTCAGCTAGAAAGAGATGAAGATGAACAAGGAGATGATGAAGAGATGGTCAGTTTGCAAGTTCCTGATCAGGAAGAGGAAGATCTTAACAGAATTAAGGAGGAAAGTAGGAGGAGAAAGCAAGCAATCTTGGAAAAATATAAAATTCAGCAGTCACAGTCCCAGCAAAAGACCGACGTACAAATTGAGTCACAGTTACAGCAACCCACTCAATTAGAGGGTCAAAAAGATCATCTGGATCAATCAACAGGGGTTGCCAAATCCGGAGCAGAAGTTGATGGTGCTAGGAGTTCTGGAGGAGTATATATGGCTGAAGCATTTTCTACTACAGCTCGTCAGAATGGTGCCCCAGGGCTTGGAGAGGGTACTCCAAAGAGTGAAAGCTCTGGTGGCTCAGAACCCAAAAAAGAAAGAGCTGATGGTATTTTATGCGATGATATCTTTGGTGAGACCCCAACTGGAGTTCGAAAATTGGACAAAGGAGATGCTGTACGAATTGAAAGGAGTGGTCTCCGTGACAACTGGGATGATGCAGAAGGGTACTACAGCTACCGGTTTGGGGAGTTACTTGATGGTCGATATGAGATTACTGCTGCTCATGGAAAAGGCGTGTTTTCAACTGTTGTTCGTGCAAAGAACCTTAAGGTTGGTAATGGTGAACCAGAAGAAGTGGCAATTAAGATTATTCGTAGCAATGATACTATGTACAAGGCTGGTATGACTGAGTTGGCCATATTGAAGAAATTAGTAGGTCAAGATCCAGACAATAAGCGCTATTGTGTTCGTTTTCTATCAAGTTTCAAGTATCGAAATCATCTTTGTTTAGTTTTTGAGTCTCTGCATATGAATCTTCGTGAGCTTTTAAAGAAGTTTGGACGCAATATTGGTCTGAATCTAACTGCGGTGAGAGTGTATGCTAAGCAACTCTTGATTGCATTGAAGCATCTCAAAAACTGTGGTGTTCTTCATTGTGATATAAAGCCAGATAACATGCTGGTAAATGAAGCAAAAAACGTTCTGAAGCTTTGTGATTTTGGTAACGCCATGTTTGCTGGCAAGAATGAAGTTACACCGTACCTTGTGAGCCGATTTTATCGGGCCCCTGAAATAATTCTTGGCTTGCCTTACGATCATCCACTGGATATCTGGTCTGTTGGTTGCTGTTTGTTCGAACTTTATACTGGAAAAGTTCTTTTTCCTGGTGCTACGAACAATGATATGCTACGTCTTCACATGGAATTGAAAGGCCCCTTCCCTAAGAAGATGCTTCGGAAGGCAACATTTACTTATCAAAATTTCGATCAGGATCTGAACTTCCATGCCACAGAAGATGATCCTGTTACAAAAAAGGTTATTAAACGCGTGATTCTCAACATAAAGCCAAAAGATATCGGATCAATGATCGCAGGTTCTCCTGGCGAGGATCCAGATCCCAAGATATTAGCTGATTTTAAAGATCTTTTAGATAAAATGTTTGTGCTGGATCCAGACAAGAGGTTGACAGTATCACAAGGAGTACTCATTACTGGCTGGTTCTTTCAGTGGAGTGTACTGTTTCTATTGATATCTGCCACTGCTTTGAGGCAACCCTCCAGTGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016310 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase TBPIP

Protein Analysis

1052

Amino Acids

120.59

Weight (kDa)

7.24

Isoelectric Point (pI)

58.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	713 - 1022	1.2e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	718 - 928	4.7e-19	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002761)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13350 AT1G13350 AT1G13350 AT3G25840 AT3G25840 AT3G53640
fragaria_vesca	FvH4_4g27640 FvH4_4g27640 FvH4_4g27640 FvH4_4g27640 FvH4_4g27640 FvH4_4g27640 FvH4_4g27640
malus_domestica	MD13G1043800.v1.1 MD16G1044600.v1.1
prunus_persica	Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1 Prupe.1G308700_v2.0.a1
pyrus_communis	pycom16g03900
rosa_chinensis	RchiOBHm_Chr4g0435481
rosa_laevigata	RLG00000006589
rosa_multiflora	Rmu_sc0001940.1_g000027
rosa_roxburghii	Rroxscaffold_5G00376710
rosa_rugosa	Rorug04G0285400 Rorug04G0285500
rosa_samantha	Rh4AG341100 Rh4BG349300 Rh4CG364000 Rh4DG343600
rosa_wichuraiana	Rw4G029760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	246
Acc36I	ACCTGC	1 cut(s)	2951
AccB1I	GGYRCC	5 cut(s)	272, 320, 588, 808, 1898
AccB7I	CCANNNNNTGG	1 cut(s)	1910
AccII	CGCG	8 cut(s)	25, 42, 138, 161, 285, 357, 735, 2916
AccIII	TCCGGA	1 cut(s)	1817
AclI	AACGTT	2 cut(s)	361, 2568
AcoI	YGGCCR	4 cut(s)	156, 230, 234, 2283
AcsI	RAATTY	6 cut(s)	258, 505, 571, 1553, 1698, 2848
AcuI	CTGAAG	2 cut(s)	1887, 2594
AcyI	GRCGYC	2 cut(s)	60, 243
AfaI	GTAC	9 cut(s)	400, 1734, 1921, 2046, 2096, 2261, 2630, 3072, 3105
AfeI	AGCGCT	1 cut(s)	2327
AfiI	CCNNNNNNNGG	5 cut(s)	470, 614, 1384, 1910, 2113
AflII	CTTAAG	1 cut(s)	2195
AflIII	ACRYGT	1 cut(s)	2914
AgeI	ACCGGT	1 cut(s)	2106
AgsI	TTSAA	9 cut(s)	341, 800, 1533, 2054, 2172, 2293, 2356, 2494, 2797
AjiI	CACGTC	1 cut(s)	1169
AjnI	CCWGG	7 cut(s)	346, 455, 591, 1521, 1903, 2752, 2968
AjuI	GAANNNNNNNTTGG	2 cut(s)	2652, 2684
Alw21I	GWGCWC	1 cut(s)	416
Alw26I	GTCTC	6 cut(s)	483, 865, 1464, 1997, 2069, 2394
AlwNI	CAGNNNCTG	1 cut(s)	2863
Ama87I	CYCGRG	1 cut(s)	1467
Aor13HI	TCCGGA	1 cut(s)	1817
Aor51HI	AGCGCT	1 cut(s)	2327
AoxI	GGCC	8 cut(s)	156, 230, 234, 489, 918, 2283, 2652, 2800
ApaI	GGGCCC	1 cut(s)	2656
ApeKI	GCWGC	2 cut(s)	126, 2147
ApoI	RAATTY	6 cut(s)	258, 505, 571, 1553, 1698, 2848
AsiGI	ACCGGT	1 cut(s)	2106
Asp700I	GAANNNNTTC	1 cut(s)	2664
AspLEI	GCGC	4 cut(s)	81, 138, 735, 2328
AspS9I	GGNCC	4 cut(s)	603, 2652, 2653, 2801
AsuII	TTCGAA	2 cut(s)	2020, 2724
AvaI	CYCGRG	1 cut(s)	1467
AvaII	GGWCC	1 cut(s)	603
BaeGI	GKGCMC	2 cut(s)	1903, 2656
BalI	TGGCCA	1 cut(s)	2285
BamHI	GGATCC	2 cut(s)	2977, 3037
BanI	GGYRCC	5 cut(s)	272, 320, 588, 808, 1898
BanII	GRGCYC	2 cut(s)	416, 2656
BauI	CACGAG	1 cut(s)	522
BbsI	GAAGAC	1 cut(s)	2774
Bbv12I	GWGCWC	1 cut(s)	416
BbvI	GCAGC	2 cut(s)	138, 2134
BccI	CCATC	6 cut(s)	820, 1520, 1594, 1826, 1967, 2120
BceAI	ACGGC	1 cut(s)	307
BciT130I	CCWGG	7 cut(s)	348, 457, 593, 1523, 1905, 2754, 2970
BclI	TGATCA	3 cut(s)	760, 1543, 1621
BcoDI	GTCTC	6 cut(s)	483, 865, 1464, 1997, 2069, 2394
BfaI	CTAG	5 cut(s)	510, 816, 1073, 1562, 1839
BfmI	CTRYAG	4 cut(s)	640, 697, 1881, 2098
BfoI	RGCGCY	2 cut(s)	82, 2329
BfrI	CTTAAG	1 cut(s)	2195
BfuAI	ACCTGC	1 cut(s)	2951
BglI	GCCNNNNNGGC	1 cut(s)	229
BglII	AGATCT	4 cut(s)	776, 824, 1636, 3010
BisI	GCNGC	9 cut(s)	40, 127, 156, 159, 191, 587, 736, 921, 2148
BlpI	GCTNAGC	1 cut(s)	2472
BlsI	GCNGC	9 cut(s)	41, 128, 157, 160, 192, 588, 737, 922, 2149
BmcAI	AGTACT	1 cut(s)	3072
Bme1390I	CCNGG	7 cut(s)	348, 457, 593, 1523, 1905, 2754, 2970
Bme18I	GGWCC	1 cut(s)	603
BmeT110I	CYCGRG	1 cut(s)	1467
BmgBI	CACGTC	1 cut(s)	1169
BmgT120I	GGNCC	4 cut(s)	603, 2652, 2653, 2801
BmrFI	CCNGG	7 cut(s)	348, 457, 593, 1523, 1905, 2754, 2970
BmrI	ACTGGG	1 cut(s)	2086
BmsI	GCATC	4 cut(s)	2029, 2074, 2506, 2808
BmuI	ACTGGG	1 cut(s)	2086
BoxI	GACNNNNGTC	1 cut(s)	1416
BpiI	GAAGAC	1 cut(s)	2774
BplI	GAGNNNNNCTC	2 cut(s)	56, 88
BpmI	CTGGAG	4 cut(s)	369, 1869, 2034, 3130
Bpu1102I	GCTNAGC	1 cut(s)	2472
Bpu14I	TTCGAA	2 cut(s)	2020, 2724
BpuEI	CTTGAG	2 cut(s)	284, 572
BsaBI	GATNNNNATC	5 cut(s)	450, 1338, 1344, 2859, 2982
BsaHI	GRCGYC	2 cut(s)	60, 243
BsaI	GGTCTC	2 cut(s)	1997, 2069
BsaJI	CCNNGG	8 cut(s)	159, 227, 237, 402, 423, 455, 1903, 1904
BsaWI	WCCGGW	3 cut(s)	1377, 1817, 2106
BsaXI	ACNNNNNCTCC	4 cut(s)	1812, 1842, 2028, 2058
Bsc4I	CCNNNNNNNGG	5 cut(s)	470, 614, 1384, 1910, 2113
Bse118I	RCCGGY	4 cut(s)	232, 275, 491, 2106
Bse1I	ACTGG	6 cut(s)	2017, 2081, 2697, 2740, 3085, 3147
Bse3DI	GCAATG	1 cut(s)	2254
Bse8I	GATNNNNATC	5 cut(s)	450, 1338, 1344, 2859, 2982
BseAI	TCCGGA	1 cut(s)	1817
BseBI	CCWGG	7 cut(s)	348, 457, 593, 1523, 1905, 2754, 2970
BseDI	CCNNGG	8 cut(s)	159, 227, 237, 402, 423, 455, 1903, 1904
BseGI	GGATG	7 cut(s)	661, 961, 1270, 1402, 1531, 2086, 2686
BseJI	GATNNNNATC	5 cut(s)	450, 1338, 1344, 2859, 2982
BseLI	CCNNNNNNNGG	5 cut(s)	470, 614, 1384, 1910, 2113
BseMI	GCAATG	1 cut(s)	2254
BseMII	CTCAG	4 cut(s)	897, 909, 1962, 2268
BseNI	ACTGG	6 cut(s)	2017, 2081, 2697, 2740, 3085, 3147
BseRI	GAGGAG	7 cut(s)	171, 174, 216, 818, 1039, 1681, 1866
BseSI	GKGCMC	2 cut(s)	1903, 2656
BseX3I	CGGCCG	3 cut(s)	156, 230, 234
BseXI	GCAGC	2 cut(s)	138, 2134
BseYI	CCCAGC	1 cut(s)	1716
Bsh1236I	CGCG	8 cut(s)	25, 42, 138, 161, 285, 357, 735, 2916
Bsh1285I	CGRYCG	3 cut(s)	159, 233, 237
BshFI	GGCC	8 cut(s)	158, 232, 236, 491, 920, 2285, 2654, 2802
BshNI	GGYRCC	5 cut(s)	272, 320, 588, 808, 1898
BshTI	ACCGGT	1 cut(s)	2106
BsiEI	CGRYCG	3 cut(s)	159, 233, 237
BsiHKAI	GWGCWC	1 cut(s)	416
BsiHKCI	CYCGRG	1 cut(s)	1467
BsiSI	CCGG	7 cut(s)	6, 233, 276, 492, 1378, 1818, 2107
BslFI	GGGAC	8 cut(s)	396, 589, 1160, 1247, 1262, 1376, 1424, 1699
BslI	CCNNNNNNNGG	5 cut(s)	470, 614, 1384, 1910, 2113
BsmAI	GTCTC	6 cut(s)	483, 865, 1464, 1997, 2069, 2394
BsmBI	CGTCTC	1 cut(s)	865
BsmFI	GGGAC	8 cut(s)	396, 589, 1160, 1247, 1262, 1376, 1424, 1699
BsnI	GGCC	8 cut(s)	158, 232, 236, 491, 920, 2285, 2654, 2802
Bso31I	GGTCTC	2 cut(s)	1997, 2069
BsoBI	CYCGRG	1 cut(s)	1467
Bsp119I	TTCGAA	2 cut(s)	2020, 2724
Bsp120I	GGGCCC	1 cut(s)	2652
Bsp1286I	GDGCHC	3 cut(s)	416, 1903, 2656
Bsp13I	TCCGGA	1 cut(s)	1817
Bsp1407I	TGTACA	1 cut(s)	2259
Bsp1720I	GCTNAGC	1 cut(s)	2472
Bsp19I	CCATGG	1 cut(s)	402
Bsp68I	TCGCGA	2 cut(s)	285, 357
BspANI	GGCC	8 cut(s)	158, 232, 236, 491, 920, 2285, 2654, 2802
BspCNI	CTCAG	4 cut(s)	898, 908, 1961, 2269
BspEI	TCCGGA	1 cut(s)	1817
BspFNI	CGCG	8 cut(s)	25, 42, 138, 161, 285, 357, 735, 2916
BspHI	TCATGA	1 cut(s)	880
BspMI	ACCTGC	1 cut(s)	2951
BspQI	GCTCTTC	1 cut(s)	1209
BspT104I	TTCGAA	2 cut(s)	2020, 2724
BspT107I	GGYRCC	5 cut(s)	272, 320, 588, 808, 1898
BspTI	CTTAAG	1 cut(s)	2195
BspTNI	GGTCTC	2 cut(s)	1997, 2069
BsrDI	GCAATG	1 cut(s)	2254
BsrFI	RCCGGY	4 cut(s)	232, 275, 491, 2106
BsrGI	TGTACA	1 cut(s)	2259
BsrI	ACTGG	6 cut(s)	2017, 2081, 2697, 2740, 3085, 3147
BssAI	RCCGGY	4 cut(s)	232, 275, 491, 2106
BssECI	CCNNGG	8 cut(s)	159, 227, 237, 402, 423, 455, 1903, 1904
BssNI	GRCGYC	2 cut(s)	60, 243
BssSI	CACGAG	1 cut(s)	522
BssT1I	CCWWGG	2 cut(s)	402, 423
Bst2BI	CACGAG	1 cut(s)	522
Bst2UI	CCWGG	7 cut(s)	348, 457, 593, 1523, 1905, 2754, 2970
Bst6I	CTCTTC	7 cut(s)	138, 575, 795, 843, 1209, 1590, 1623
BstACI	GRCGYC	2 cut(s)	60, 243
BstAFI	CTTAAG	1 cut(s)	2195
BstAUI	TGTACA	1 cut(s)	2259
BstBI	TTCGAA	2 cut(s)	2020, 2724
BstC8I	GCNNGC	9 cut(s)	32, 234, 493, 497, 1295, 1299, 1677, 2608, 2675
BstDEI	CTNAG	7 cut(s)	895, 906, 1948, 2277, 2472, 2811, 3153
BstDSI	CCRYGG	2 cut(s)	159, 402
BstENI	CCTNNNNNAGG	1 cut(s)	612
BstF5I	GGATG	7 cut(s)	661, 961, 1270, 1402, 1531, 2086, 2686
BstFNI	CGCG	8 cut(s)	25, 42, 138, 161, 285, 357, 735, 2916
BstH2I	RGCGCY	2 cut(s)	82, 2329
BstHHI	GCGC	4 cut(s)	81, 138, 735, 2328
BstMAI	GTCTC	6 cut(s)	483, 865, 1464, 1997, 2069, 2394
BstMCI	CGRYCG	3 cut(s)	159, 233, 237
BstNI	CCWGG	7 cut(s)	348, 457, 593, 1523, 1905, 2754, 2970
BstNSI	RCATGY	1 cut(s)	2548
BstPAI	GACNNNNGTC	1 cut(s)	1416
BstSCI	CCNGG	7 cut(s)	346, 455, 591, 1521, 1903, 2752, 2968
BstSFI	CTRYAG	4 cut(s)	640, 697, 1881, 2098
BstSLI	GKGCMC	2 cut(s)	1903, 2656
BstUI	CGCG	8 cut(s)	25, 42, 138, 161, 285, 357, 735, 2916
BstV1I	GCAGC	2 cut(s)	138, 2134
BstV2I	GAAGAC	1 cut(s)	2774
BstX2I	RGATCY	9 cut(s)	776, 824, 1636, 2311, 2860, 2977, 2983, 3010, 3037
BstYI	RGATCY	9 cut(s)	776, 824, 1636, 2311, 2860, 2977, 2983, 3010, 3037
BstZI	CGGCCG	3 cut(s)	156, 230, 234
BsuRI	GGCC	8 cut(s)	158, 232, 236, 491, 920, 2285, 2654, 2802
BtgI	CCRYGG	2 cut(s)	159, 402
BtgZI	GCGATG	1 cut(s)	2001
BtrI	CACGTC	1 cut(s)	1169
BtsCI	GGATG	7 cut(s)	661, 961, 1270, 1402, 1531, 2086, 2686
BtsI	GCAGTG	1 cut(s)	3126
BtsIMutI	CAGTG	5 cut(s)	1179, 2690, 3101, 3126, 3154
BtuMI	TCGCGA	2 cut(s)	285, 357
BveI	ACCTGC	1 cut(s)	2951
Cac8I	GCNNGC	9 cut(s)	32, 234, 493, 497, 1295, 1299, 1677, 2608, 2675
CaiI	CAGNNNCTG	1 cut(s)	2863
CciI	TCATGA	1 cut(s)	880
CciNI	GCGGCCGC	1 cut(s)	156
CfoI	GCGC	4 cut(s)	81, 138, 735, 2328
Cfr10I	RCCGGY	4 cut(s)	232, 275, 491, 2106
Cfr13I	GGNCC	4 cut(s)	603, 2652, 2653, 2801
Cfr42I	CCGCGG	1 cut(s)	162
CseI	GACGC	3 cut(s)	14, 68, 2439
Csp6I	GTAC	9 cut(s)	399, 1733, 1920, 2045, 2095, 2260, 2629, 3071, 3104
CspAI	ACCGGT	1 cut(s)	2106
CspCI	CAANNNNNGTGG	2 cut(s)	2491, 2526
CviAII	CATG	9 cut(s)	403, 560, 764, 881, 2153, 2545, 2599, 2788, 2873
CviQI	GTAC	9 cut(s)	399, 1733, 1920, 2045, 2095, 2260, 2629, 3071, 3104
DdeI	CTNAG	7 cut(s)	895, 906, 1948, 2277, 2472, 2811, 3153
DraI	TTTAAA	2 cut(s)	2418, 3007
EaeI	YGGCCR	4 cut(s)	156, 230, 234, 2283
EagI	CGGCCG	3 cut(s)	156, 230, 234
Eam1104I	CTCTTC	7 cut(s)	138, 575, 795, 843, 1209, 1590, 1623
EarI	CTCTTC	7 cut(s)	138, 575, 795, 843, 1209, 1590, 1623
EciI	GGCGGA	2 cut(s)	177, 210
Ecl136II	GAGCTC	1 cut(s)	414
EclXI	CGGCCG	3 cut(s)	156, 230, 234
Eco130I	CCWWGG	2 cut(s)	402, 423
Eco24I	GRGCYC	2 cut(s)	416, 2656
Eco31I	GGTCTC	2 cut(s)	1997, 2069
Eco32I	GATATC	5 cut(s)	172, 1993, 2698, 2944, 3120
Eco47I	GGWCC	1 cut(s)	603
Eco47III	AGCGCT	1 cut(s)	2327
Eco52I	CGGCCG	3 cut(s)	156, 230, 234
Eco53kI	GAGCTC	1 cut(s)	414
Eco57I	CTGAAG	2 cut(s)	1887, 2594
Eco88I	CYCGRG	1 cut(s)	1467
EcoICRI	GAGCTC	1 cut(s)	414
EcoNI	CCTNNNNNAGG	1 cut(s)	612
EcoO109I	RGGNCCY	3 cut(s)	603, 2653, 2801
EcoRII	CCWGG	7 cut(s)	346, 455, 591, 1521, 1903, 2752, 2968
EcoRV	GATATC	5 cut(s)	172, 1993, 2698, 2944, 3120
EcoT14I	CCWWGG	2 cut(s)	402, 423
EcoT38I	GRGCYC	2 cut(s)	416, 2656
ErhI	CCWWGG	2 cut(s)	402, 423
Esp3I	CGTCTC	1 cut(s)	865
FaeI	CATG	9 cut(s)	406, 563, 767, 884, 2156, 2548, 2602, 2791, 2876
FalI	AAGNNNNNCTT	2 cut(s)	2465, 2497
FaqI	GGGAC	8 cut(s)	396, 589, 1160, 1247, 1262, 1376, 1424, 1699
FatI	CATG	9 cut(s)	402, 559, 763, 880, 2152, 2544, 2598, 2787, 2872
FauI	CCCGC	2 cut(s)	154, 1386
FauNDI	CATATG	1 cut(s)	2397
FbaI	TGATCA	3 cut(s)	760, 1543, 1621
Fnu4HI	GCNGC	9 cut(s)	40, 127, 156, 159, 191, 587, 736, 921, 2148
FokI	GGATG	7 cut(s)	668, 968, 1277, 1409, 1538, 2093, 2673
FriOI	GRGCYC	2 cut(s)	416, 2656
FseI	GGCCGGCC	1 cut(s)	236
Fsp4HI	GCNGC	9 cut(s)	40, 127, 156, 159, 191, 587, 736, 921, 2148
FspBI	CTAG	5 cut(s)	510, 816, 1073, 1562, 1839
GlaI	GCGC	4 cut(s)	80, 137, 734, 2327
GluI	GCNGC	9 cut(s)	40, 127, 156, 159, 191, 587, 736, 921, 2148
GsaI	CCCAGC	1 cut(s)	1720
GsuI	CTGGAG	4 cut(s)	369, 1869, 2034, 3130
HaeII	RGCGCY	2 cut(s)	82, 2329
HaeIII	GGCC	8 cut(s)	158, 232, 236, 491, 920, 2285, 2654, 2802
HapII	CCGG	7 cut(s)	6, 233, 276, 492, 1378, 1818, 2107
HgaI	GACGC	3 cut(s)	14, 68, 2439
HhaI	GCGC	4 cut(s)	81, 138, 735, 2328
Hin1I	GRCGYC	2 cut(s)	60, 243
Hin1II	CATG	9 cut(s)	406, 563, 767, 884, 2156, 2548, 2602, 2791, 2876
Hin6I	GCGC	4 cut(s)	79, 136, 733, 2326
HinP1I	GCGC	4 cut(s)	79, 136, 733, 2326
HincII	GTYRAC	2 cut(s)	547, 3054
HindII	GTYRAC	2 cut(s)	547, 3054
HindIII	AAGCTT	1 cut(s)	2576
HpaII	CCGG	7 cut(s)	6, 233, 276, 492, 1378, 1818, 2107
Hpy166II	GTNNAC	5 cut(s)	547, 1045, 2213, 3054, 3104
Hpy8I	GTNNAC	5 cut(s)	547, 1045, 2213, 3054, 3104
Hpy99I	CGWCG	9 cut(s)	14, 30, 56, 59, 62, 248, 290, 293, 1733
HpyCH4IV	ACGT	7 cut(s)	243, 361, 874, 1168, 1731, 2568, 2779
HpyCH4V	TGCA	5 cut(s)	1612, 2087, 2186, 2395, 2489
HpyF3I	CTNAG	7 cut(s)	895, 906, 1948, 2277, 2472, 2811, 3153
HpySE526I	ACGT	7 cut(s)	243, 361, 874, 1168, 1731, 2568, 2779
Hsp92I	GRCGYC	2 cut(s)	60, 243
Hsp92II	CATG	9 cut(s)	406, 563, 767, 884, 2156, 2548, 2602, 2791, 2876
HspAI	GCGC	4 cut(s)	79, 136, 733, 2326
KflI	GGGWCCC	1 cut(s)	603
Kpn2I	TCCGGA	1 cut(s)	1817
KroI	GCCGGC	2 cut(s)	232, 491
KroNI	GCCGGC	2 cut(s)	234, 493
Ksp22I	TGATCA	3 cut(s)	760, 1543, 1621
KspI	CCGCGG	1 cut(s)	162
LguI	GCTCTTC	1 cut(s)	1209
LmnI	GCTCC	5 cut(s)	184, 349, 741, 1278, 1820
Lsp1109I	GCAGC	2 cut(s)	138, 2134
LweI	GCATC	4 cut(s)	2029, 2074, 2506, 2808
MaeI	CTAG	5 cut(s)	510, 816, 1073, 1562, 1839
MaeII	ACGT	7 cut(s)	243, 361, 874, 1168, 1731, 2568, 2779
MflI	RGATCY	9 cut(s)	776, 824, 1636, 2311, 2860, 2977, 2983, 3010, 3037
MhlI	GDGCHC	3 cut(s)	416, 1903, 2656
MlsI	TGGCCA	1 cut(s)	2285
MluI	ACGCGT	1 cut(s)	2914
MluNI	TGGCCA	1 cut(s)	2285
MlyI	GAGTC	5 cut(s)	308, 335, 469, 1751, 2396
MmeI	TCCRAC	1 cut(s)	75
Mox20I	TGGCCA	1 cut(s)	2285
MroI	TCCGGA	1 cut(s)	1817
MroNI	GCCGGC	2 cut(s)	232, 491
MroXI	GAANNNNTTC	1 cut(s)	2664
MscI	TGGCCA	1 cut(s)	2285
MseI	TTAA	7 cut(s)	1641, 1650, 2196, 2232, 2417, 2910, 3006
MslI	CAYNNNNRTG	1 cut(s)	564
Msp20I	TGGCCA	1 cut(s)	2285
MspA1I	CMGCKG	1 cut(s)	161
MspCI	CTTAAG	1 cut(s)	2195
MspI	CCGG	7 cut(s)	6, 233, 276, 492, 1378, 1818, 2107
MspR9I	CCNGG	7 cut(s)	348, 457, 593, 1523, 1905, 2754, 2970
MvaI	CCWGG	7 cut(s)	348, 457, 593, 1523, 1905, 2754, 2970
MvnI	CGCG	8 cut(s)	25, 42, 138, 161, 285, 357, 735, 2916
NaeI	GCCGGC	2 cut(s)	234, 493
NcoI	CCATGG	1 cut(s)	402
NdeI	CATATG	1 cut(s)	2397
NgoMIV	GCCGGC	2 cut(s)	232, 491
NlaIII	CATG	9 cut(s)	406, 563, 767, 884, 2156, 2548, 2602, 2791, 2876
NmeAIII	GCCGAG	5 cut(s)	88, 208, 262, 349, 541
NmuCI	GTSAC	9 cut(s)	92, 113, 392, 690, 710, 1127, 1707, 1743, 2069
NotI	GCGGCCGC	1 cut(s)	156
NruI	TCGCGA	2 cut(s)	285, 357
NspI	RCATGY	1 cut(s)	2548
NspV	TTCGAA	2 cut(s)	2020, 2724
PaeR7I	CTCGAG	1 cut(s)	1467
PagI	TCATGA	1 cut(s)	880
PasI	CCCWGGG	1 cut(s)	1904
PciSI	GCTCTTC	1 cut(s)	1209
PcsI	WCGNNNNNNNCGW	1 cut(s)	60
PdiI	GCCGGC	2 cut(s)	234, 493
PdmI	GAANNNNTTC	1 cut(s)	2664
PfeI	GAWTC	6 cut(s)	20, 530, 596, 2401, 2446, 2920
PflMI	CCANNNNNTGG	1 cut(s)	1910
PfoI	TCCNGGA	1 cut(s)	346
PinAI	ACCGGT	1 cut(s)	2106
PkrI	GCNGC	9 cut(s)	41, 128, 157, 160, 192, 588, 737, 922, 2149
PleI	GAGTC	5 cut(s)	308, 334, 468, 1750, 2395
PpsI	GAGTC	5 cut(s)	308, 334, 468, 1750, 2395
PpuMI	RGGWCCY	1 cut(s)	603
PshAI	GACNNNNGTC	1 cut(s)	1416
Psp124BI	GAGCTC	1 cut(s)	416
Psp1406I	AACGTT	2 cut(s)	361, 2568
Psp5II	RGGWCCY	1 cut(s)	603
Psp6I	CCWGG	7 cut(s)	346, 455, 591, 1521, 1903, 2752, 2968
PspFI	CCCAGC	1 cut(s)	1716
PspGI	CCWGG	7 cut(s)	346, 455, 591, 1521, 1903, 2752, 2968
PspOMI	GGGCCC	1 cut(s)	2652
PspPI	GGNCC	4 cut(s)	603, 2652, 2653, 2801
PspPPI	RGGWCCY	1 cut(s)	603
PstNI	CAGNNNCTG	1 cut(s)	2863
PsuI	RGATCY	9 cut(s)	776, 824, 1636, 2311, 2860, 2977, 2983, 3010, 3037
RigI	GGCCGGCC	1 cut(s)	236
RruI	TCGCGA	2 cut(s)	285, 357
RsaI	GTAC	9 cut(s)	400, 1734, 1921, 2046, 2096, 2261, 2630, 3072, 3105
RsaNI	GTAC	9 cut(s)	399, 1733, 1920, 2045, 2095, 2260, 2629, 3071, 3104
RseI	CAYNNNNRTG	1 cut(s)	564
SacI	GAGCTC	1 cut(s)	416
SacII	CCGCGG	1 cut(s)	162
SapI	GCTCTTC	1 cut(s)	1209
SaqAI	TTAA	7 cut(s)	1641, 1650, 2196, 2232, 2417, 2910, 3006
SatI	GCNGC	9 cut(s)	40, 127, 156, 159, 191, 587, 736, 921, 2148
Sau96I	GGNCC	4 cut(s)	603, 2652, 2653, 2801
ScaI	AGTACT	1 cut(s)	3072
SchI	GAGTC	5 cut(s)	308, 335, 469, 1751, 2396
ScrFI	CCNGG	7 cut(s)	348, 457, 593, 1523, 1905, 2754, 2970
SduI	GDGCHC	3 cut(s)	416, 1903, 2656
SfaNI	GCATC	4 cut(s)	2029, 2074, 2506, 2808
SfcI	CTRYAG	4 cut(s)	640, 697, 1881, 2098
Sfr274I	CTCGAG	1 cut(s)	1467
Sfr303I	CCGCGG	1 cut(s)	162
SfuI	TTCGAA	2 cut(s)	2020, 2724
SgrBI	CCGCGG	1 cut(s)	162
SinI	GGWCC	1 cut(s)	603
SlaI	CTCGAG	1 cut(s)	1467
SmiMI	CAYNNNNRTG	1 cut(s)	564
SmlI	CTYRAG	4 cut(s)	263, 551, 1467, 2195
SmoI	CTYRAG	4 cut(s)	263, 551, 1467, 2195
SspI	AATATT	1 cut(s)	2437
SspMI	CTAG	5 cut(s)	510, 816, 1073, 1562, 1839
SstI	GAGCTC	1 cut(s)	416
StyD4I	CCNGG	7 cut(s)	346, 455, 591, 1521, 1903, 2752, 2968
StyI	CCWWGG	2 cut(s)	402, 423
TaiI	ACGT	7 cut(s)	246, 364, 877, 1171, 1734, 2571, 2782
TaqII	GACCGA	1 cut(s)	1742
TatI	WGTACW	3 cut(s)	2259, 3070, 3103
TauI	GCSGC	7 cut(s)	42, 158, 161, 193, 589, 738, 923
TfiI	GAWTC	6 cut(s)	20, 530, 596, 2401, 2446, 2920
Tru1I	TTAA	7 cut(s)	1641, 1650, 2196, 2232, 2417, 2910, 3006
Tru9I	TTAA	7 cut(s)	1641, 1650, 2196, 2232, 2417, 2910, 3006
TscAI	CASTG	5 cut(s)	1186, 2697, 3101, 3133, 3154
TseFI	GTSAC	9 cut(s)	92, 113, 392, 690, 710, 1127, 1707, 1743, 2069
TseI	GCWGC	2 cut(s)	126, 2147
Tsp45I	GTSAC	9 cut(s)	92, 113, 392, 690, 710, 1127, 1707, 1743, 2069
TspGWI	ACGGA	2 cut(s)	1214, 2057
TspRI	CASTG	5 cut(s)	1186, 2697, 3101, 3133, 3154
Van91I	CCANNNNNTGG	1 cut(s)	1910
Vha464I	CTTAAG	1 cut(s)	2195
VpaK11BI	GGWCC	1 cut(s)	603
XagI	CCTNNNNNAGG	1 cut(s)	612
XapI	RAATTY	6 cut(s)	258, 505, 571, 1553, 1698, 2848
XbaI	TCTAGA	1 cut(s)	815
XceI	RCATGY	1 cut(s)	2548
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2697
XhoI	CTCGAG	1 cut(s)	1467
XmnI	GAANNNNTTC	1 cut(s)	2664
XspI	CTAG	5 cut(s)	510, 816, 1073, 1562, 1839
ZraI	GACGTC	1 cut(s)	244
ZrmI	AGTACT	1 cut(s)	3072

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.