Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0007740

Physical Location & Seq

Genomic Coordinates Forward (+)

3380093 .. 3385934

Gene Structure 5842 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1048400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2799 bp

ATGAGCAGCAGAGCCAGAAACTTCCGCCGCCGCGCCGACGATGACGATGATCAAAACGACGACGCAACCCCCTCAACGGCAACCACCGTCAAATCCTCGTTAAAACCCTCTTCCGCATCCTCCTCAAAGCCCAAGAAGCCACAGAATCAAGGCCCGAAGCTCCTCAGCTTTGTCGACGACGAAGAAAACGCCGCCGCGCCGTCCCGCTCCGCGCCATCTTCCAAACGCGATAAGCCCTCTTCTCGTCTGGGCAAGCCCTCTTCAGCTCATAAGTTGACGGCTCTCAAAGACCGTCTCGCTCATTCGTCTTCTGTATCGACTTCTCTGCCGTCCAACGTTCAGCCCCAAGCCGGAACCTATACGAAAGAAGCGCTCCGTGAGCTGCAGAAGAACACGCGAACCCTAGCCAGCTCCCGACCCTCCTCCGAACCCACCATTGTTCTGAAGGGCCTTGTCAAACCCACCGACTCAATCTCCGATACGCTGAGAGAGTCACGGGAGTTGGATTCCGATTCCGACGAGAAGCATGAGAAGGAAAGGGGTAATTTGTTTAGGAGGGACAAGGACGAAGCAGAGGCCCAATTGGCCTCGATGAGAATTGACAGGGGCAAGAGGTCACCTGGGTTGTTTCCTGATCAAGCCACCATAGATGCCATTCGGGCCAAGAGGGAACGGCTCAGGAAATCGAGGGCAGCGGCTCCGGACTTTATCTCATTGGATTCAGGGAGTAATCATGGCGCTGCAGAAGGGTTGAGCGATGAGGAGCCAGAGTTTCGAGGTCGAATTGCAATGTTTGGGGATAAGATGGACGGTTCTAAAAAGGGTGTTTTTGAGGATGTTGATGATGGGGTAGTGGATGCTGGATTGAGGCAGGAAAGCGTCGATCAGGATGAGGAAGAGGATGAGGATGAGGAAGATAAGATTTGGGAAGAAGAGCAGTTTAGGAAAGGGTTGGGGAAGAGGGTGGATGACGGGGTGGTCAGCACTAGTGCTCCTGCAATTCCGAGTGTGCCCCAACCAAAGACTACTTACTCTGCAATGACGGGTTATAGTTGGTCAGAGAGTATGCCTCTTGGGACAAGTATAGGAGGGGCAATTGGAGCTTCACAAGGTTCAAATGTGATGTCCATAAAGGCACAGGCGGACACTGCTAGAAAAGCTTTGGAAGAAAATTTCACGAAGCTTAAGGCAAGTCATGGAAGAACCATTTTGTCACTAACTAAGACTGACGAAAATTTGTCGGCCTCACTGTTGAATATTACTGCTCTCGAGAAGTCTCTGTCTGCTGCTGATGAGAAGTACCAGTTCATGCAAAAGCTTCGCGATTTTGTCTCTGCCATATGTGACTTTCTGCAGGTTAAAGCTCCCTATATAGAAGAGCTTGAAGCGGAAATGCAGAAAGTTCATGAACAACGTGCCTCATCTACTATGGAAAGGAGAAATGCTGATGATGATGAAATGATGGAAATAGAAGCAGCTGTAAAAGCAGCAATGTCTATCATCAGTAAAGAAGGTAGCAGTGCTGAAATAATTGCAGCTGCTAAAAGTGCAGCACAAGCTGCATCTACTGCTGTGAGAGAACAAACTAATCTACCAGTGAAGTTAGATGAATTTGGTAGAGATATGAACCTTAAGAAACGTATGGATATGAAAGTGAGGTATGAGGCTCGACAACGCAGGAAAAGAACGTGTGAATCTAAGAGACTGTCAGCCATGGATGTGGACAGTTCCCATCGAACAATAGAAGGAGAATCAAGCACCGATGAGAGTGACAGCGAGAGTCAAGTATACCACAGACACCGCCAGTTGGTGCTCGACACTGCAGGTAAAGTATTCGATGATGCAGCAGAGGAATATTCTCAACTTTCAGTGGTCAAAGAAAGGTTTGAAGACTGGAAGAGAGAGTATGCATCAAGCTACCGTGATGCTTACATGTCTTTAAGTGTTCCTACTATCTTCTCTCCATATGTGAGACTGGAGCTTTTGAAGTGGGACCCCCTCCGTGATAAAACAGATTTTCTAAACATGAACTGGCATTCATTGTTAATGGACTATGGTTTACTGGAGGATGGAAATGATTTGGCATCAGATGATGCTGATGCTAACCTTGTCCCTGATCTGGTGGAAAAGGTTGCTTTACCCATTTTACATCACCAAGTTGCTCACTGTTGGAACATTCTCAGTGTGCGAGAGACCAAGAACGCAGTTGCTGCCACAAGTTTGGTAACAGATTATGTTCCACCTTCTAGTGAGGCCCTTGCTGATTTATTAGTTGCTGTTCGCACCCGTCTGGCTGATGCTGTCACAAATCTTACGGTCCCAACATGGAGCCCAATTGTATTGACAGCAGTGCCAGATGCGGCACGGCTGGCAGCATATCGATTCGGTATGTCTGTTCGTTTAATGAAAAATATCTGCTTGTGGAAAGAAATCCTTGCTTCTCCCATTTTAGAGAAGCTTGCTATTGAGGAGCTGTTGTGTGGTAAAGTTCTACCTCATGTTCGAAGCATTGCTGCAAATGTTCATGATGCTGTTACAAGAATTGAAAGGATTGTTGCTTCTTTATCTGGGGTGTGGGCAGGCTCAAATGTCACAGGGGACAGCAGCCGTAAGCTGCAACCGCTGGTGGATTATGTGCTCTCGTTGGGTAGGACTCTGGAGAAGAAGCATTTGCTTGGCGTGACACAAAGTGAAACTGGTGGACTTGCCCGTAGATTAAAGAAAATGCTGGTTGATCTGAACCAGTATGACAAGGCTAGGGACATAGCAAGGACTTTCAACCTTAAGGAGGCATTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0000375 GO:0000377 GO:0000398 GO:0000976 GO:0000977 GO:0000978 GO:0000981 GO:0000982 GO:0000987 GO:0001012 GO:0001067 GO:0001078 GO:0001227 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006355 GO:0006357 GO:0006396 GO:0006397 GO:0006417 GO:0006725 GO:0006807 GO:0007049 GO:0008150 GO:0008152 GO:0008380 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0017148 GO:0019219 GO:0019222 GO:0022402 GO:0030371 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0034641 GO:0034645 GO:0042023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0045182 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0140110 GO:1901360 GO:1901363 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GCFC NTR2

Protein Analysis

933

Amino Acids

103.07

Weight (kDa)

5.75

Isoelectric Point (pI)

47.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NTR2	PF15458	119 - 341	1.5e-07	Nineteen complex-related protein 2
GCFC	PF07842	623 - 826	9.6e-31	GC-rich sequence DNA-binding factor-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007740)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08550
fragaria_vesca	FvH4_4g28100 FvH4_4g28100 FvH4_4g28100 FvH4_4g28100 FvH4_4g28100
malus_domestica	MD13G1046700.v1.1 MD16G1048400.v1.1
prunus_persica	Prupe.1G305200_v2.0.a1 Prupe.1G305200_v2.0.a1 Prupe.1G305200_v2.0.a1 Prupe.1G305200_v2.0.a1
pyrus_communis	pycom13g04090 pycom16g04230
rosa_chinensis	RchiOBHm_Chr4g0435851
rosa_laevigata	RLG00000006554
rosa_multiflora	Rmu_sc0002642.1_g000001
rosa_roxburghii	Rroxscaffold_5G00377030
rosa_rugosa	Rorug04G0290000
rosa_samantha	Rh4AG344300 Rh4BG353200 Rh4CG367600 Rh4DG347100
rosa_wichuraiana	Rw4G030080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	977
Acc36I	ACCTGC	2 cut(s)	1345, 1814
AccBSI	CCGCTC	1 cut(s)	207
AccI	GTMKAC	2 cut(s)	174, 1788
AccII	CGCG	6 cut(s)	33, 197, 212, 228, 397, 1323
AccIII	TCCGGA	1 cut(s)	700
AclI	AACGTT	1 cut(s)	336
AcsI	RAATTY	3 cut(s)	1171, 1234, 1610
AcuI	CTGAAG	2 cut(s)	246, 464
AdeI	CACNNNGTG	1 cut(s)	2690
AfaI	GTAC	1 cut(s)	1301
AfeI	AGCGCT	1 cut(s)	372
AfiI	CCNNNNNNNGG	5 cut(s)	76, 350, 1807, 2119, 2788
AflII	CTTAAG	3 cut(s)	1184, 1631, 2783
AflIII	ACRYGT	2 cut(s)	1688, 1932
AgsI	TTSAA	7 cut(s)	1116, 1255, 1385, 1889, 1987, 2546, 2779
AhlI	ACTAGT	1 cut(s)	986
AjnI	CCWGG	1 cut(s)	619
Alw21I	GWGCWC	3 cut(s)	994, 1815, 2640
Alw26I	GTCTC	6 cut(s)	299, 1281, 1336, 1696, 1966, 2186
AlwNI	CAGNNNCTG	1 cut(s)	2210
Ama87I	CYCGRG	1 cut(s)	1268
Aor13HI	TCCGGA	1 cut(s)	700
Aor51HI	AGCGCT	1 cut(s)	372
AoxI	GGCC	7 cut(s)	151, 448, 576, 585, 660, 1242, 2253
ApoI	RAATTY	3 cut(s)	1171, 1234, 1610
ArsI	GACNNNNNNTTYG	2 cut(s)	1109, 1141
AspLEI	GCGC	5 cut(s)	35, 199, 214, 373, 740
AspS9I	GGNCC	7 cut(s)	152, 448, 577, 660, 1993, 2254, 2317
AsuHPI	GGTGA	2 cut(s)	609, 2144
AsuII	TTCGAA	1 cut(s)	2503
AvaI	CYCGRG	1 cut(s)	1268
AvaII	GGWCC	2 cut(s)	1993, 2317
BaeGI	GKGCMC	1 cut(s)	1014
BanII	GRGCYC	1 cut(s)	2333
BbsI	GAAGAC	2 cut(s)	300, 1896
Bbv12I	GWGCWC	3 cut(s)	994, 1815, 2640
BbvCI	CCTCAGC	1 cut(s)	164
BccI	CCATC	6 cut(s)	223, 799, 839, 1456, 1740, 2063
BceAI	ACGGC	7 cut(s)	93, 184, 294, 313, 689, 2381, 2592
BcgI	CGANNNNNNTGC	4 cut(s)	307, 341, 1301, 1335
BciT130I	CCWGG	1 cut(s)	621
BclI	TGATCA	2 cut(s)	49, 634
BcoDI	GTCTC	6 cut(s)	299, 1281, 1336, 1696, 1966, 2186
BcuI	ACTAGT	1 cut(s)	986
BfaI	CTAG	5 cut(s)	404, 987, 1152, 2247, 2757
BfmI	CTRYAG	4 cut(s)	383, 741, 1352, 1821
BfoI	RGCGCY	2 cut(s)	374, 741
BfrI	CTTAAG	3 cut(s)	1184, 1631, 2783
BfuAI	ACCTGC	2 cut(s)	1345, 1814
BglI	GCCNNNNNGGC	2 cut(s)	584, 659
Bme1390I	CCNGG	1 cut(s)	621
Bme18I	GGWCC	2 cut(s)	1993, 2317
BmeT110I	CYCGRG	1 cut(s)	1268
BmgT120I	GGNCC	7 cut(s)	152, 448, 577, 660, 1993, 2254, 2317
BmiI	GGNNCC	7 cut(s)	355, 699, 765, 1994, 1995, 2319, 2330
BmrFI	CCNGG	1 cut(s)	621
BpiI	GAAGAC	2 cut(s)	300, 1896
BplI	GAGNNNNNCTC	2 cut(s)	1054, 1086
BpmI	CTGGAG	3 cut(s)	1997, 2084, 2678
Bpu10I	CCTNAGC	2 cut(s)	164, 677
Bpu14I	TTCGAA	1 cut(s)	2503
Bsa29I	ATCGAT	1 cut(s)	2380
BsaI	GGTCTC	1 cut(s)	2186
BsaJI	CCNNGG	2 cut(s)	620, 1713
BsaWI	WCCGGW	1 cut(s)	700
BsaXI	ACNNNNNCTCC	9 cut(s)	458, 488, 755, 785, 976, 1006, 2462, 2492, 2780
Bsc4I	CCNNNNNNNGG	5 cut(s)	76, 350, 1807, 2119, 2788
Bse1I	ACTGG	9 cut(s)	1303, 1595, 1804, 1898, 1980, 2036, 2067, 2701, 2743
Bse3DI	GCAATG	4 cut(s)	795, 1044, 1497, 2508
BseAI	TCCGGA	1 cut(s)	700
BseBI	CCWGG	1 cut(s)	621
BseCI	ATCGAT	1 cut(s)	2380
BseDI	CCNNGG	2 cut(s)	620, 1713
BseGI	GGATG	9 cut(s)	116, 841, 862, 895, 907, 913, 973, 1723, 2074
BseLI	CCNNNNNNNGG	5 cut(s)	76, 350, 1807, 2119, 2788
BseMI	GCAATG	4 cut(s)	795, 1044, 1497, 2508
BseMII	CTCAG	4 cut(s)	178, 476, 691, 2194
BseNI	ACTGG	9 cut(s)	1303, 1595, 1804, 1898, 1980, 2036, 2067, 2701, 2743
BseRI	GAGGAG	5 cut(s)	112, 152, 412, 776, 2483
BseSI	GKGCMC	1 cut(s)	1014
BsgI	GTGCAG	1 cut(s)	1569
Bsh1236I	CGCG	6 cut(s)	33, 197, 212, 228, 397, 1323
BshFI	GGCC	7 cut(s)	153, 450, 578, 587, 662, 1244, 2255
BshVI	ATCGAT	1 cut(s)	2380
BsiHKAI	GWGCWC	3 cut(s)	994, 1815, 2640
BsiHKCI	CYCGRG	1 cut(s)	1268
BsiSI	CCGG	2 cut(s)	351, 701
BslFI	GGGAC	8 cut(s)	187, 572, 1090, 2006, 2096, 2303, 2612, 2774
BslI	CCNNNNNNNGG	5 cut(s)	76, 350, 1807, 2119, 2788
BsmAI	GTCTC	6 cut(s)	299, 1281, 1336, 1696, 1966, 2186
BsmBI	CGTCTC	1 cut(s)	299
BsmFI	GGGAC	8 cut(s)	187, 572, 1090, 2006, 2096, 2303, 2612, 2774
BsmI	GAATGC	1 cut(s)	2035
BsnI	GGCC	7 cut(s)	153, 450, 578, 587, 662, 1244, 2255
Bso31I	GGTCTC	1 cut(s)	2186
BsoBI	CYCGRG	1 cut(s)	1268
Bsp119I	TTCGAA	1 cut(s)	2503
Bsp1286I	GDGCHC	5 cut(s)	994, 1014, 1815, 2333, 2640
Bsp13I	TCCGGA	1 cut(s)	700
Bsp143I	GATC	5 cut(s)	49, 634, 883, 2116, 2734
Bsp19I	CCATGG	1 cut(s)	1713
Bsp68I	TCGCGA	1 cut(s)	1323
BspANI	GGCC	7 cut(s)	153, 450, 578, 587, 662, 1244, 2255
BspCNI	CTCAG	4 cut(s)	177, 477, 690, 2193
BspDI	ATCGAT	1 cut(s)	2380
BspEI	TCCGGA	1 cut(s)	700
BspFNI	CGCG	6 cut(s)	33, 197, 212, 228, 397, 1323
BspHI	TCATGA	2 cut(s)	1405, 2524
BspLI	GGNNCC	7 cut(s)	355, 699, 765, 1994, 1995, 2319, 2330
BspMAI	CTGCAG	4 cut(s)	387, 745, 1356, 1825
BspMI	ACCTGC	2 cut(s)	1345, 1814
BspQI	GCTCTTC	2 cut(s)	927, 1371
BspT104I	TTCGAA	1 cut(s)	2503
BspTI	CTTAAG	3 cut(s)	1184, 1631, 2783
BspTNI	GGTCTC	1 cut(s)	2186
BsrBI	CCGCTC	1 cut(s)	207
BsrDI	GCAATG	4 cut(s)	795, 1044, 1497, 2508
BsrI	ACTGG	9 cut(s)	1303, 1595, 1804, 1898, 1980, 2036, 2067, 2701, 2743
BssECI	CCNNGG	2 cut(s)	620, 1713
BssMI	GATC	5 cut(s)	49, 634, 883, 2116, 2734
BssNAI	GTATAC	1 cut(s)	1789
BssT1I	CCWWGG	1 cut(s)	1713
Bst1107I	GTATAC	1 cut(s)	1789
Bst2UI	CCWGG	1 cut(s)	621
Bst4CI	ACNGT	9 cut(s)	88, 293, 812, 1251, 1707, 1727, 1922, 2168, 2317
Bst6I	CTCTTC	8 cut(s)	115, 244, 265, 891, 927, 953, 1371, 1892
BstAFI	CTTAAG	3 cut(s)	1184, 1631, 2783
BstAPI	GCANNNNNTGC	3 cut(s)	1559, 1568, 2210
BstBI	TTCGAA	1 cut(s)	2503
BstC8I	GCNNGC	5 cut(s)	254, 409, 2370, 2460, 2581
BstDEI	CTNAG	6 cut(s)	164, 485, 677, 1221, 1698, 2180
BstDSI	CCRYGG	1 cut(s)	1713
BstEII	GGTNACC	1 cut(s)	615
BstENI	CCTNNNNNAGG	1 cut(s)	2786
BstF5I	GGATG	9 cut(s)	116, 841, 862, 895, 907, 913, 973, 1723, 2074
BstFNI	CGCG	6 cut(s)	33, 197, 212, 228, 397, 1323
BstH2I	RGCGCY	2 cut(s)	374, 741
BstHHI	GCGC	5 cut(s)	35, 199, 214, 373, 740
BstKTI	GATC	5 cut(s)	52, 637, 886, 2119, 2737
BstMAI	GTCTC	6 cut(s)	299, 1281, 1336, 1696, 1966, 2186
BstMBI	GATC	5 cut(s)	49, 634, 883, 2116, 2734
BstNI	CCWGG	1 cut(s)	621
BstNSI	RCATGY	1 cut(s)	1936
BstPI	GGTNACC	1 cut(s)	615
BstSCI	CCNGG	1 cut(s)	619
BstSFI	CTRYAG	4 cut(s)	383, 741, 1352, 1821
BstSLI	GKGCMC	1 cut(s)	1014
BstUI	CGCG	6 cut(s)	33, 197, 212, 228, 397, 1323
BstV2I	GAAGAC	2 cut(s)	300, 1896
BstXI	CCANNNNNNTGG	2 cut(s)	1720, 2221
BstZ17I	GTATAC	1 cut(s)	1789
Bsu15I	ATCGAT	1 cut(s)	2380
BsuRI	GGCC	7 cut(s)	153, 450, 578, 587, 662, 1244, 2255
BsuTUI	ATCGAT	1 cut(s)	2380
BtgI	CCRYGG	1 cut(s)	1713
BtgZI	GCGATG	1 cut(s)	771
BtsCI	GGATG	9 cut(s)	116, 841, 862, 895, 907, 913, 973, 1723, 2074
BtsI	GCAGTG	4 cut(s)	1146, 1525, 1818, 2355
BtsIMutI	CAGTG	9 cut(s)	1146, 1247, 1525, 1602, 1818, 1875, 2164, 2188, 2355
BtuMI	TCGCGA	1 cut(s)	1323
BveI	ACCTGC	2 cut(s)	1345, 1814
Cac8I	GCNNGC	5 cut(s)	254, 409, 2370, 2460, 2581
CaiI	CAGNNNCTG	1 cut(s)	2210
CciI	TCATGA	2 cut(s)	1405, 2524
CfoI	GCGC	5 cut(s)	35, 199, 214, 373, 740
Cfr13I	GGNCC	7 cut(s)	152, 448, 577, 660, 1993, 2254, 2317
ClaI	ATCGAT	1 cut(s)	2380
CseI	GACGC	2 cut(s)	71, 868
Csp6I	GTAC	1 cut(s)	1300
CviQI	GTAC	1 cut(s)	1300
DdeI	CTNAG	6 cut(s)	164, 485, 677, 1221, 1698, 2180
DpnI	GATC	5 cut(s)	51, 636, 885, 2118, 2736
DpnII	GATC	5 cut(s)	49, 634, 883, 2116, 2734
DraIII	CACNNNGTG	1 cut(s)	2690
DrdI	GACNNNNNNGTC	1 cut(s)	977
DseDI	GACNNNNNNGTC	1 cut(s)	977
Eam1104I	CTCTTC	8 cut(s)	115, 244, 265, 891, 927, 953, 1371, 1892
EarI	CTCTTC	8 cut(s)	115, 244, 265, 891, 927, 953, 1371, 1892
EciI	GGCGGA	2 cut(s)	14, 1157
Eco130I	CCWWGG	1 cut(s)	1713
Eco24I	GRGCYC	1 cut(s)	2333
Eco31I	GGTCTC	1 cut(s)	2186
Eco47I	GGWCC	2 cut(s)	1993, 2317
Eco47III	AGCGCT	1 cut(s)	372
Eco57I	CTGAAG	2 cut(s)	246, 464
Eco88I	CYCGRG	1 cut(s)	1268
Eco91I	GGTNACC	1 cut(s)	615
EcoNI	CCTNNNNNAGG	1 cut(s)	2786
EcoO109I	RGGNCCY	3 cut(s)	448, 1993, 2254
EcoO65I	GGTNACC	1 cut(s)	615
EcoRII	CCWGG	1 cut(s)	619
EcoT14I	CCWWGG	1 cut(s)	1713
EcoT22I	ATGCAT	1 cut(s)	1912
EcoT38I	GRGCYC	1 cut(s)	2333
ErhI	CCWWGG	1 cut(s)	1713
Esp3I	CGTCTC	1 cut(s)	299
FalI	AAGNNNNNCTT	2 cut(s)	2418, 2450
FaqI	GGGAC	8 cut(s)	187, 572, 1090, 2006, 2096, 2303, 2612, 2774
FauI	CCCGC	1 cut(s)	212
FauNDI	CATATG	2 cut(s)	1340, 1966
FbaI	TGATCA	2 cut(s)	49, 634
FblI	GTMKAC	2 cut(s)	174, 1788
FokI	GGATG	9 cut(s)	103, 848, 869, 902, 914, 920, 980, 1730, 2081
FriOI	GRGCYC	1 cut(s)	2333
FspBI	CTAG	5 cut(s)	404, 987, 1152, 2247, 2757
GlaI	GCGC	5 cut(s)	34, 198, 213, 372, 739
GsuI	CTGGAG	3 cut(s)	1997, 2084, 2678
HaeII	RGCGCY	2 cut(s)	374, 741
HaeIII	GGCC	7 cut(s)	153, 450, 578, 587, 662, 1244, 2255
HapII	CCGG	2 cut(s)	351, 701
HgaI	GACGC	2 cut(s)	71, 868
HhaI	GCGC	5 cut(s)	35, 199, 214, 373, 740
Hin6I	GCGC	5 cut(s)	33, 197, 212, 371, 738
HinP1I	GCGC	5 cut(s)	33, 197, 212, 371, 738
HincII	GTYRAC	2 cut(s)	175, 276
HindII	GTYRAC	2 cut(s)	175, 276
HindIII	AAGCTT	4 cut(s)	1158, 1181, 1316, 2456
HpaII	CCGG	2 cut(s)	351, 701
HphI	GGTGA	2 cut(s)	609, 2144
Hpy166II	GTNNAC	6 cut(s)	175, 276, 1723, 1789, 2060, 2702
Hpy188I	TCNGA	9 cut(s)	427, 444, 478, 511, 517, 1005, 1060, 2089, 2739
Hpy8I	GTNNAC	6 cut(s)	175, 276, 1723, 1789, 2060, 2702
Hpy99I	CGWCG	7 cut(s)	41, 62, 65, 179, 182, 521, 884
HpyAV	CCTTC	6 cut(s)	439, 526, 740, 1505, 1739, 2253
HpyCH4III	ACNGT	9 cut(s)	88, 293, 812, 1251, 1707, 1727, 1922, 2168, 2317
HpyCH4IV	ACGT	4 cut(s)	336, 1414, 1639, 1688
HpyF3I	CTNAG	6 cut(s)	164, 485, 677, 1221, 1698, 2180
HpySE526I	ACGT	4 cut(s)	336, 1414, 1639, 1688
HspAI	GCGC	5 cut(s)	33, 197, 212, 371, 738
KflI	GGGWCCC	1 cut(s)	1993
Kpn2I	TCCGGA	1 cut(s)	700
Ksp22I	TGATCA	2 cut(s)	49, 634
Kzo9I	GATC	5 cut(s)	49, 634, 883, 2116, 2734
LguI	GCTCTTC	2 cut(s)	927, 1371
MaeI	CTAG	5 cut(s)	404, 987, 1152, 2247, 2757
MaeII	ACGT	4 cut(s)	336, 1414, 1639, 1688
MalI	GATC	5 cut(s)	51, 636, 885, 2118, 2736
MbiI	CCGCTC	1 cut(s)	207
MboI	GATC	5 cut(s)	49, 634, 883, 2116, 2734
MfeI	CAATTG	3 cut(s)	581, 1095, 2334
MhlI	GDGCHC	5 cut(s)	994, 1014, 1815, 2333, 2640
MlyI	GAGTC	4 cut(s)	461, 500, 1789, 2647
MmeI	TCCRAC	4 cut(s)	357, 483, 540, 2150
Mph1103I	ATGCAT	1 cut(s)	1912
MroI	TCCGGA	1 cut(s)	700
MseI	TTAA	9 cut(s)	101, 1185, 1359, 1632, 1940, 2045, 2402, 2717, 2784
MslI	CAYNNNNRTG	1 cut(s)	1718
MspA1I	CMGCKG	4 cut(s)	695, 1478, 1538, 2623
MspCI	CTTAAG	3 cut(s)	1184, 1631, 2783
MspI	CCGG	2 cut(s)	351, 701
MspR9I	CCNGG	1 cut(s)	621
MunI	CAATTG	3 cut(s)	581, 1095, 2334
Mva1269I	GAATGC	1 cut(s)	2035
MvaI	CCWGG	1 cut(s)	621
MvnI	CGCG	6 cut(s)	33, 197, 212, 228, 397, 1323
NcoI	CCATGG	1 cut(s)	1713
NdeI	CATATG	2 cut(s)	1340, 1966
NdeII	GATC	5 cut(s)	49, 634, 883, 2116, 2734
NlaIV	GGNNCC	7 cut(s)	355, 699, 765, 1994, 1995, 2319, 2330
NmuCI	GTSAC	8 cut(s)	492, 615, 1212, 1343, 1769, 2302, 2590, 2680
NruI	TCGCGA	1 cut(s)	1323
NsiI	ATGCAT	1 cut(s)	1912
NspI	RCATGY	1 cut(s)	1936
NspV	TTCGAA	1 cut(s)	2503
PaeR7I	CTCGAG	1 cut(s)	1268
PagI	TCATGA	2 cut(s)	1405, 2524
PciI	ACATGT	1 cut(s)	1932
PciSI	GCTCTTC	2 cut(s)	927, 1371
PctI	GAATGC	1 cut(s)	2035
PfeI	GAWTC	7 cut(s)	145, 506, 512, 719, 1694, 1751, 2382
PleI	GAGTC	4 cut(s)	461, 499, 1788, 2647
PpsI	GAGTC	4 cut(s)	461, 499, 1788, 2647
PpuMI	RGGWCCY	1 cut(s)	1993
PscI	ACATGT	1 cut(s)	1932
Psp1406I	AACGTT	1 cut(s)	336
Psp5II	RGGWCCY	1 cut(s)	1993
Psp6I	CCWGG	1 cut(s)	619
PspEI	GGTNACC	1 cut(s)	615
PspGI	CCWGG	1 cut(s)	619
PspN4I	GGNNCC	7 cut(s)	355, 699, 765, 1994, 1995, 2319, 2330
PspPI	GGNCC	7 cut(s)	152, 448, 577, 660, 1993, 2254, 2317
PspPPI	RGGWCCY	1 cut(s)	1993
PstI	CTGCAG	4 cut(s)	387, 745, 1356, 1825
PstNI	CAGNNNCTG	1 cut(s)	2210
PvuII	CAGCTG	2 cut(s)	1478, 1538
RruI	TCGCGA	1 cut(s)	1323
RsaI	GTAC	1 cut(s)	1301
RsaNI	GTAC	1 cut(s)	1300
RseI	CAYNNNNRTG	1 cut(s)	1718
SalI	GTCGAC	1 cut(s)	173
SapI	GCTCTTC	2 cut(s)	927, 1371
SaqAI	TTAA	9 cut(s)	101, 1185, 1359, 1632, 1940, 2045, 2402, 2717, 2784
Sau3AI	GATC	5 cut(s)	49, 634, 883, 2116, 2734
Sau96I	GGNCC	7 cut(s)	152, 448, 577, 660, 1993, 2254, 2317
SchI	GAGTC	4 cut(s)	461, 500, 1789, 2647
ScrFI	CCNGG	1 cut(s)	621
SduI	GDGCHC	5 cut(s)	994, 1014, 1815, 2333, 2640
SfcI	CTRYAG	4 cut(s)	383, 741, 1352, 1821
SfiI	GGCCNNNNNGGCC	1 cut(s)	584
Sfr274I	CTCGAG	1 cut(s)	1268
SfuI	TTCGAA	1 cut(s)	2503
SinI	GGWCC	2 cut(s)	1993, 2317
SlaI	CTCGAG	1 cut(s)	1268
SmiMI	CAYNNNNRTG	1 cut(s)	1718
SmlI	CTYRAG	4 cut(s)	1184, 1268, 1631, 2783
SmoI	CTYRAG	4 cut(s)	1184, 1268, 1631, 2783
SpeI	ACTAGT	1 cut(s)	986
SspI	AATATT	2 cut(s)	1258, 1856
SspMI	CTAG	5 cut(s)	404, 987, 1152, 2247, 2757
StyD4I	CCNGG	1 cut(s)	619
StyI	CCWWGG	1 cut(s)	1713
TaaI	ACNGT	9 cut(s)	88, 293, 812, 1251, 1707, 1727, 1922, 2168, 2317
TaiI	ACGT	4 cut(s)	339, 1417, 1642, 1691
TauI	GCSGC	6 cut(s)	30, 33, 194, 197, 698, 2363
TfiI	GAWTC	7 cut(s)	145, 506, 512, 719, 1694, 1751, 2382
Tru1I	TTAA	9 cut(s)	101, 1185, 1359, 1632, 1940, 2045, 2402, 2717, 2784
Tru9I	TTAA	9 cut(s)	101, 1185, 1359, 1632, 1940, 2045, 2402, 2717, 2784
TscAI	CASTG	9 cut(s)	1153, 1254, 1525, 1602, 1825, 1875, 2171, 2188, 2355
TseFI	GTSAC	8 cut(s)	492, 615, 1212, 1343, 1769, 2302, 2590, 2680
Tsp45I	GTSAC	8 cut(s)	492, 615, 1212, 1343, 1769, 2302, 2590, 2680
TspGWI	ACGGA	2 cut(s)	365, 1991
TspRI	CASTG	9 cut(s)	1153, 1254, 1525, 1602, 1825, 1875, 2171, 2188, 2355
Vha464I	CTTAAG	3 cut(s)	1184, 1631, 2783
VpaK11BI	GGWCC	2 cut(s)	1993, 2317
XagI	CCTNNNNNAGG	1 cut(s)	2786
XapI	RAATTY	3 cut(s)	1171, 1234, 1610
XceI	RCATGY	1 cut(s)	1936
XhoI	CTCGAG	1 cut(s)	1268
XmiI	GTMKAC	2 cut(s)	174, 1788
XspI	CTAG	5 cut(s)	404, 987, 1152, 2247, 2757
Zsp2I	ATGCAT	1 cut(s)	1912

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.