Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr13

Orthogroup ID

OG0007740

Physical Location & Seq

Genomic Coordinates Forward (+)

2730785 .. 2736014

Gene Structure 5230 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom13g04090.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2907 bp

ATGCATCGCGTTTTGCGAAAAAAAAAAAAACGGAAAAAGAAGTGGAAGAGATTTCTAACCAATGACCAGACCAGGGCAGTAGTGAATGCTGCAATCCCTAAACCCGCCACCGCCACCATGAGCAGCAGATCCAGAAACTTCCGCCGCCGCGCCGACGACGGCGCCGATCAAAACGACGACGCAAATGACACCACAACCGCAGTTAAATCCTCATCAAAACCCTCTTCCGCCTCCTCGTCAAAGCCCAAGAAGCCACAGAACCAAGCCCCGAAGCTTCTCAGCTTCGTTGACGACGAAGAAAACGCAGCCGCCCCGTCCCGCTCCGCCTCTTCTTCCAAACGCGAGAAGCCCTCCTCCCGTCTCGGCAAGCCTTCTTCAGCTCATAAGTTGACGGCTCTCAAGGACCGTCTCGCTCATTCGTCTTCCGCGTCCACTTCCGTGCCGTCCAACGTTCAGCCACAAGCAGGTACCTACACGAAAGAAGCGCTCCGTGAGCTGCAGAAGAACACGCGAACCCTAGCCAGCTCCCGACCTTCCTCCGAGCCCACCATTGTTCTGAAGGGCCTTGTCAAACCCACGGACTCAATCTCCGATACGCTGAGAAAAGCCCGGGAGTTGGACTCCGATTCCGACGAGAAGCATGAGAAGGAAAGGGGTAGTTTGTTTAATAGGGAAAAGGACGATGCGGAGGCCCGATTGGCCTCGATGGGAATTGACAAGGGCAAGAGCTCACCTGGTTTGTTTCCTGATCAAGCCACCATCGATGCAATTCGGGCAAAGAGGGAACGGCTGAGGAAATCGAGGGCAGCAGCCCCGGATTTTATCTCATTGGATTCAGGGAGTAATCATGGCGCTGCGGAAGGATTGAGTGATGAGGAACCAGAGTTTCGAGGCAGAATTGCAATGTTTGGGGATAAGATGGACGGTTCTAAAAAGGGGGTCTTTGAGGATGTTGATGATAGGGCAGTGGATGCTGGATTGAGGCAGGAAAGTGTCGAAGATGGTGATGAAGAGGATGAGGAAGAGAAGATTTGGGAAGAAGAGCAGTTTAGGAAAGGGTTGGGAAAGAGGGTGGATGACGGGGTGGTCAGTACTAGCGCTCCTGCGGTTCCTAGTGTGCCCCAGCCAAAGGCTACCTACTCTGCAGTGACGGGTTATAGTTTGGCACAGAGTGTGCCTGTTGGGCCAAGTATAGGAGGGGCAATTGGAGCATCACAAGGTTCGAATGTGATGTCCATAAAGGCGCAGGCGGACATTGCTAGGAAAGCTTTGGAAGAAAATTTCATGAAGCTTAAGGCAAGTCATGGCAGAACCATGTTGTCACTGACTAAGACTGATGAAAATTTGTCTGCCTCGCTGTTGAATATTACTGCTCTCGAGAAGTCTCTGTCTGCTGCTGATGAGAAGTACCAGTTCATGCAAAAGCTTCGCGATTTTGTCTCTTCCATATGTGACTTTTTGCAGGTTAAAGCTCCCTATATAGAAGAGCTTGAAGCAGAAATGCAGAAAGTTCATGAACAACGTGCGTCATCTACTTTGGAGAGGAGAAGTGCTGATGATGATGAAATGATGGAAGTAGAAGCAGCTGTAAAAGCAGCAATGTCTATCTTCAGTAAAGAAGGTAGCAGTGCTGAAGTAATTGCAGCTGCTAAAAGTGCAGCACAAGAGGCATCTGCTGCTTTGAGAGAACAAACAAATCTACCAGTGAAGTTAGACGAATTTGGTAGAGATATGAACCTTAAGAAACGTATGGATATGAAAGGGAGGTATGAGGCTCGACAACGCAGGAAAGTACGGTACGACTCTAAGAGACTGTCATCCATGGATGTGGATAGTTCCCATCGAACAATAGAAGGAGAATCAAGCACTGATGAGAGTGACAGTGAGAGTCAAGTATACCATAAACACCGCCAGTTGGTGCTCGACACTGCAGGTAAAGTATTCGATGATGCAGCAGAGGAATATTCTCAACTTTCAGTGGTCAAAGAAAGGTTTGAAGACTGGAAGAGAGAGTATGCATCAAGCTACCGTGATGCTTATATGTCTTTAAGTGTTCCTACTATTTTCTCTCCATATGTGCGGCTGGAGCTTTTGAAGTGGGACCCCCTCCGTGATAAAACAGATTTTCTAAACATGACCTGGCATTCATTGTTAATGGACTATGGTTTACTGGAGGATGGAAATGATTTGGCATCAGATGATGCTGATGCTAACCTTGTCCCTGATCTGGTGGAAAAGGTTGCTTTACCCATTTTGCATCACCAAGTTGTTTACTGTTGGAACATTCTCAGTATGCGAGAGACAGAGAATGCAGTTGCTGCCACAAGTTTGGTAACAGATTACGTTCCACCTTCTAGTGAGGCTCTTGCAGATTTATTAGGTGCTATTCGCACCCGTCTGGCTGATGCTGTCACTAATCTTACGGTCCCGACATGGAGCCCGCTTGTATTGACAGCAGTGCCAGATGCGGCACGGCTGGCAGCATATCGATTCGGTATGTCTGTTCGTTTGATGAAAAATATCTGCTTGTGGAAGGAAATCCTTGCTTCTCCCATTCTAGAGAAGCTTGCCATTGAGGAGCTGTTGTGTGGTAAAGTTCTACCTCATGTTCAAAGCATTGCCGCAAATGTGCATGATGCTGTTACAAGAACTGAAAGGATTGTTGCTTCTCTATCTGGGGTGTGGGCAGGTTCAAACCTCACAGGGGACCGCAGCCGTAAGTTGCAATCGCTGGTGGATTATGTGCTCTCATTGGGTAGGACTCTGGAGAAGAAGCATTCGCTTGGTGTGACACAAAGTGAAACTGGTGGACTTGCCCGTAGATTAAAGAAAATGCTGGTTGATCTGAACCAGTATGACAAGGCTAGGGACATAGCAAGGACATTCAATCTTAAGGAGGCATTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0000375 GO:0000377 GO:0000398 GO:0000976 GO:0000977 GO:0000978 GO:0000981 GO:0000982 GO:0000987 GO:0001012 GO:0001067 GO:0001078 GO:0001227 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006355 GO:0006357 GO:0006396 GO:0006397 GO:0006417 GO:0006725 GO:0006807 GO:0007049 GO:0008150 GO:0008152 GO:0008380 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0017148 GO:0019219 GO:0019222 GO:0022402 GO:0030371 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0034641 GO:0034645 GO:0042023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0045182 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0140110 GO:1901360 GO:1901363 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GCFC NTR2

Protein Analysis

969

Amino Acids

106.9

Weight (kDa)

7.28

Isoelectric Point (pI)

46.45

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007740)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08550
fragaria_vesca	FvH4_4g28100 FvH4_4g28100 FvH4_4g28100 FvH4_4g28100 FvH4_4g28100
malus_domestica	MD13G1046700.v1.1 MD16G1048400.v1.1
prunus_persica	Prupe.1G305200_v2.0.a1 Prupe.1G305200_v2.0.a1 Prupe.1G305200_v2.0.a1 Prupe.1G305200_v2.0.a1
pyrus_communis	pycom13g04090 pycom16g04230
rosa_chinensis	RchiOBHm_Chr4g0435851
rosa_laevigata	RLG00000006554
rosa_multiflora	Rmu_sc0002642.1_g000001
rosa_roxburghii	Rroxscaffold_5G00377030
rosa_rugosa	Rorug04G0290000
rosa_samantha	Rh4AG344300 Rh4BG353200 Rh4CG367600 Rh4DG347100
rosa_wichuraiana	Rw4G030080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1085
Acc36I	ACCTGC	4 cut(s)	455, 1453, 1922, 2678
Acc65I	GGTACC	1 cut(s)	467
AccB1I	GGYRCC	2 cut(s)	161, 467
AccBSI	CCGCTC	1 cut(s)	321
AccI	GTMKAC	1 cut(s)	1896
AccII	CGCG	6 cut(s)	9, 150, 342, 428, 511, 1431
AclI	AACGTT	1 cut(s)	450
AclWI	GGATC	1 cut(s)	123
AcsI	RAATTY	3 cut(s)	1279, 1342, 1718
AcuI	CTGAAG	4 cut(s)	360, 578, 1594, 1653
AcyI	GRCGYC	1 cut(s)	162
AdeI	CACNNNGTG	2 cut(s)	1172, 2798
AfaI	GTAC	5 cut(s)	469, 1093, 1409, 1794, 1799
AfeI	AGCGCT	2 cut(s)	486, 1099
AfiI	CCNNNNNNNGG	8 cut(s)	73, 464, 616, 1129, 1193, 1915, 2227, 2704
AflII	CTTAAG	3 cut(s)	1292, 1739, 2891
AgsI	TTSAA	7 cut(s)	1363, 1493, 1997, 2095, 2612, 2694, 2887
AjnI	CCWGG	3 cut(s)	71, 733, 2138
AleI	CACNNNNGTG	1 cut(s)	437
Alw21I	GWGCWC	3 cut(s)	731, 1923, 2748
Alw26I	GTCTC	6 cut(s)	365, 413, 1389, 1444, 1804, 2294
AlwI	GGATC	1 cut(s)	123
AlwNI	CAGNNNCTG	1 cut(s)	2318
Ama87I	CYCGRG	2 cut(s)	609, 1376
Aor51HI	AGCGCT	2 cut(s)	486, 1099
AoxI	GGCC	4 cut(s)	562, 690, 699, 1184
ApoI	RAATTY	3 cut(s)	1279, 1342, 1718
ArsI	GACNNNNNNTTYG	2 cut(s)	1217, 1249
Asp718I	GGTACC	1 cut(s)	467
AspLEI	GCGC	6 cut(s)	152, 164, 487, 854, 1100, 1246
AspS9I	GGNCC	7 cut(s)	403, 562, 691, 1184, 2101, 2425, 2707
AsuC2I	CCSGG	3 cut(s)	610, 611, 815
AsuHPI	GGTGA	3 cut(s)	723, 1016, 2252
AsuII	TTCGAA	1 cut(s)	1223
AvaI	CYCGRG	2 cut(s)	609, 1376
AvaII	GGWCC	4 cut(s)	403, 2101, 2425, 2707
BaeGI	GKGCMC	1 cut(s)	1122
BanI	GGYRCC	2 cut(s)	161, 467
BanII	GRGCYC	3 cut(s)	546, 731, 2441
BbsI	GAAGAC	2 cut(s)	414, 2004
Bbv12I	GWGCWC	3 cut(s)	731, 1923, 2748
BbvCI	CCTCAGC	1 cut(s)	791
BccI	CCATC	7 cut(s)	700, 767, 913, 995, 1564, 1848, 2171
BceAI	ACGGC	6 cut(s)	175, 408, 427, 803, 2489, 2700
BcgI	CGANNNNNNTGC	2 cut(s)	1409, 1443
BciT130I	CCWGG	3 cut(s)	73, 735, 2140
BclI	TGATCA	1 cut(s)	748
BcnI	CCSGG	3 cut(s)	610, 611, 815
BcoDI	GTCTC	6 cut(s)	365, 413, 1389, 1444, 1804, 2294
BfaI	CTAG	7 cut(s)	518, 1095, 1113, 1260, 2355, 2558, 2865
BfmI	CTRYAG	3 cut(s)	497, 1143, 1929
BfoI	RGCGCY	4 cut(s)	165, 488, 855, 1101
BfrI	CTTAAG	3 cut(s)	1292, 1739, 2891
BfuAI	ACCTGC	4 cut(s)	455, 1453, 1922, 2678
BglI	GCCNNNNNGGC	2 cut(s)	698, 1183
BmcAI	AGTACT	1 cut(s)	1093
Bme1390I	CCNGG	6 cut(s)	73, 610, 611, 735, 815, 2140
Bme18I	GGWCC	4 cut(s)	403, 2101, 2425, 2707
BmeT110I	CYCGRG	2 cut(s)	609, 1376
BmgT120I	GGNCC	7 cut(s)	403, 562, 691, 1184, 2101, 2425, 2707
BmiI	GGNNCC	9 cut(s)	163, 469, 879, 1110, 2102, 2103, 2427, 2438, 2708
BmrFI	CCNGG	6 cut(s)	73, 610, 611, 735, 815, 2140
BpiI	GAAGAC	2 cut(s)	414, 2004
BplI	GAGNNNNNCTC	4 cut(s)	335, 367, 605, 637
BpmI	CTGGAG	3 cut(s)	2105, 2192, 2786
Bpu10I	CCTNAGC	1 cut(s)	791
Bpu14I	TTCGAA	1 cut(s)	1223
BpuEI	CTTGAG	1 cut(s)	383
BpuMI	CCSGG	3 cut(s)	610, 611, 815
Bsa29I	ATCGAT	2 cut(s)	762, 2488
BsaHI	GRCGYC	1 cut(s)	162
BsaJI	CCNNGG	5 cut(s)	72, 576, 609, 813, 1821
BsaXI	ACNNNNNCTCC	7 cut(s)	572, 602, 1084, 1114, 2570, 2600, 2888
Bsc4I	CCNNNNNNNGG	8 cut(s)	73, 464, 616, 1129, 1193, 1915, 2227, 2704
Bse1I	ACTGG	7 cut(s)	1411, 1703, 1912, 2006, 2175, 2809, 2851
Bse3DI	GCAATG	4 cut(s)	909, 1254, 1605, 2616
BseBI	CCWGG	3 cut(s)	73, 735, 2140
BseCI	ATCGAT	2 cut(s)	762, 2488
BseDI	CCNNGG	5 cut(s)	72, 576, 609, 813, 1821
BseGI	GGATG	7 cut(s)	955, 976, 1021, 1081, 1817, 1831, 2182
BseLI	CCNNNNNNNGG	8 cut(s)	73, 464, 616, 1129, 1193, 1915, 2227, 2704
BseMI	GCAATG	4 cut(s)	909, 1254, 1605, 2616
BseMII	CTCAG	4 cut(s)	292, 590, 782, 2302
BseNI	ACTGG	7 cut(s)	1411, 1703, 1912, 2006, 2175, 2809, 2851
BseRI	GAGGAG	4 cut(s)	223, 343, 1558, 2591
BseSI	GKGCMC	1 cut(s)	1122
BseYI	CCCAGC	1 cut(s)	1122
BsgI	GTGCAG	1 cut(s)	1677
Bsh1236I	CGCG	6 cut(s)	9, 150, 342, 428, 511, 1431
BshFI	GGCC	4 cut(s)	564, 692, 701, 1186
BshNI	GGYRCC	2 cut(s)	161, 467
BshVI	ATCGAT	2 cut(s)	762, 2488
BsiHKAI	GWGCWC	3 cut(s)	731, 1923, 2748
BsiHKCI	CYCGRG	2 cut(s)	609, 1376
BsiSI	CCGG	2 cut(s)	610, 815
BslFI	GGGAC	6 cut(s)	301, 2114, 2204, 2411, 2720, 2882
BslI	CCNNNNNNNGG	8 cut(s)	73, 464, 616, 1129, 1193, 1915, 2227, 2704
BsmAI	GTCTC	6 cut(s)	365, 413, 1389, 1444, 1804, 2294
BsmBI	CGTCTC	2 cut(s)	365, 413
BsmFI	GGGAC	6 cut(s)	301, 2114, 2204, 2411, 2720, 2882
BsmI	GAATGC	4 cut(s)	91, 2143, 2314, 2776
BsnI	GGCC	4 cut(s)	564, 692, 701, 1186
BsoBI	CYCGRG	2 cut(s)	609, 1376
Bsp119I	TTCGAA	1 cut(s)	1223
Bsp1286I	GDGCHC	6 cut(s)	546, 731, 1122, 1923, 2441, 2748
Bsp143I	GATC	5 cut(s)	128, 166, 748, 2224, 2842
Bsp19I	CCATGG	1 cut(s)	1821
Bsp68I	TCGCGA	1 cut(s)	1431
BspANI	GGCC	4 cut(s)	564, 692, 701, 1186
BspCNI	CTCAG	4 cut(s)	291, 591, 783, 2301
BspDI	ATCGAT	2 cut(s)	762, 2488
BspFNI	CGCG	6 cut(s)	9, 150, 342, 428, 511, 1431
BspHI	TCATGA	2 cut(s)	1284, 1513
BspLI	GGNNCC	9 cut(s)	163, 469, 879, 1110, 2102, 2103, 2427, 2438, 2708
BspMAI	CTGCAG	3 cut(s)	501, 1147, 1933
BspMI	ACCTGC	4 cut(s)	455, 1453, 1922, 2678
BspPI	GGATC	1 cut(s)	123
BspQI	GCTCTTC	2 cut(s)	1035, 1479
BspT104I	TTCGAA	1 cut(s)	1223
BspT107I	GGYRCC	2 cut(s)	161, 467
BspTI	CTTAAG	3 cut(s)	1292, 1739, 2891
BsrBI	CCGCTC	1 cut(s)	321
BsrDI	GCAATG	4 cut(s)	909, 1254, 1605, 2616
BsrI	ACTGG	7 cut(s)	1411, 1703, 1912, 2006, 2175, 2809, 2851
BssECI	CCNNGG	5 cut(s)	72, 576, 609, 813, 1821
BssMI	GATC	5 cut(s)	128, 166, 748, 2224, 2842
BssNAI	GTATAC	1 cut(s)	1897
BssNI	GRCGYC	1 cut(s)	162
BssT1I	CCWWGG	1 cut(s)	1821
Bst1107I	GTATAC	1 cut(s)	1897
Bst2UI	CCWGG	3 cut(s)	73, 735, 2140
Bst4CI	ACNGT	8 cut(s)	407, 926, 1797, 1815, 1883, 2030, 2276, 2425
Bst6I	CTCTTC	9 cut(s)	41, 229, 334, 1005, 1017, 1035, 1447, 1479, 2000
BstACI	GRCGYC	1 cut(s)	162
BstAFI	CTTAAG	3 cut(s)	1292, 1739, 2891
BstAPI	GCANNNNNTGC	3 cut(s)	971, 1676, 2318
BstBI	TTCGAA	1 cut(s)	1223
BstC8I	GCNNGC	6 cut(s)	368, 523, 1248, 2441, 2478, 2568
BstDEI	CTNAG	6 cut(s)	278, 599, 791, 1329, 1806, 2288
BstDSI	CCRYGG	2 cut(s)	576, 1821
BstF5I	GGATG	7 cut(s)	955, 976, 1021, 1081, 1817, 1831, 2182
BstFNI	CGCG	6 cut(s)	9, 150, 342, 428, 511, 1431
BstH2I	RGCGCY	4 cut(s)	165, 488, 855, 1101
BstHHI	GCGC	6 cut(s)	152, 164, 487, 854, 1100, 1246
BstKTI	GATC	5 cut(s)	131, 169, 751, 2227, 2845
BstMAI	GTCTC	6 cut(s)	365, 413, 1389, 1444, 1804, 2294
BstMBI	GATC	5 cut(s)	128, 166, 748, 2224, 2842
BstNI	CCWGG	3 cut(s)	73, 735, 2140
BstSCI	CCNGG	6 cut(s)	71, 608, 609, 733, 813, 2138
BstSFI	CTRYAG	3 cut(s)	497, 1143, 1929
BstSLI	GKGCMC	1 cut(s)	1122
BstUI	CGCG	6 cut(s)	9, 150, 342, 428, 511, 1431
BstV2I	GAAGAC	2 cut(s)	414, 2004
BstX2I	RGATCY	1 cut(s)	128
BstXI	CCANNNNNNTGG	2 cut(s)	1828, 2329
BstYI	RGATCY	1 cut(s)	128
BstZ17I	GTATAC	1 cut(s)	1897
Bsu15I	ATCGAT	2 cut(s)	762, 2488
BsuRI	GGCC	4 cut(s)	564, 692, 701, 1186
BsuTUI	ATCGAT	2 cut(s)	762, 2488
BtgI	CCRYGG	2 cut(s)	576, 1821
BtsCI	GGATG	7 cut(s)	955, 976, 1021, 1081, 1817, 1831, 2182
BtsI	GCAGTG	5 cut(s)	972, 1152, 1633, 1926, 2463
BtuMI	TCGCGA	1 cut(s)	1431
BveI	ACCTGC	4 cut(s)	455, 1453, 1922, 2678
Cac8I	GCNNGC	6 cut(s)	368, 523, 1248, 2441, 2478, 2568
CaiI	CAGNNNCTG	1 cut(s)	2318
CciI	TCATGA	2 cut(s)	1284, 1513
CfoI	GCGC	6 cut(s)	152, 164, 487, 854, 1100, 1246
Cfr13I	GGNCC	7 cut(s)	403, 562, 691, 1184, 2101, 2425, 2707
Cfr9I	CCCGGG	1 cut(s)	609
ClaI	ATCGAT	2 cut(s)	762, 2488
CseI	GACGC	3 cut(s)	188, 417, 1515
CsiI	ACCWGGT	1 cut(s)	733
Csp6I	GTAC	5 cut(s)	468, 1092, 1408, 1793, 1798
CviQI	GTAC	5 cut(s)	468, 1092, 1408, 1793, 1798
DdeI	CTNAG	6 cut(s)	278, 599, 791, 1329, 1806, 2288
DinI	GGCGCC	1 cut(s)	163
DpnI	GATC	5 cut(s)	130, 168, 750, 2226, 2844
DpnII	GATC	5 cut(s)	128, 166, 748, 2224, 2842
DraIII	CACNNNGTG	2 cut(s)	1172, 2798
DrdI	GACNNNNNNGTC	1 cut(s)	1085
DseDI	GACNNNNNNGTC	1 cut(s)	1085
Eam1104I	CTCTTC	9 cut(s)	41, 229, 334, 1005, 1017, 1035, 1447, 1479, 2000
EarI	CTCTTC	9 cut(s)	41, 229, 334, 1005, 1017, 1035, 1447, 1479, 2000
EciI	GGCGGA	4 cut(s)	131, 217, 313, 1265
Ecl136II	GAGCTC	1 cut(s)	729
Eco130I	CCWWGG	1 cut(s)	1821
Eco24I	GRGCYC	3 cut(s)	546, 731, 2441
Eco47I	GGWCC	4 cut(s)	403, 2101, 2425, 2707
Eco47III	AGCGCT	2 cut(s)	486, 1099
Eco53kI	GAGCTC	1 cut(s)	729
Eco57I	CTGAAG	4 cut(s)	360, 578, 1594, 1653
Eco88I	CYCGRG	2 cut(s)	609, 1376
EcoICRI	GAGCTC	1 cut(s)	729
EcoO109I	RGGNCCY	2 cut(s)	562, 2101
EcoRII	CCWGG	3 cut(s)	71, 733, 2138
EcoT14I	CCWWGG	1 cut(s)	1821
EcoT22I	ATGCAT	2 cut(s)	6, 2020
EcoT38I	GRGCYC	3 cut(s)	546, 731, 2441
EgeI	GGCGCC	1 cut(s)	163
EheI	GGCGCC	1 cut(s)	163
ErhI	CCWWGG	1 cut(s)	1821
Esp3I	CGTCTC	2 cut(s)	365, 413
FalI	AAGNNNNNCTT	2 cut(s)	926, 958
FaqI	GGGAC	6 cut(s)	301, 2114, 2204, 2411, 2720, 2882
FauI	CCCGC	3 cut(s)	112, 326, 2448
FauNDI	CATATG	2 cut(s)	1448, 2074
FbaI	TGATCA	1 cut(s)	748
FblI	GTMKAC	1 cut(s)	1896
FokI	GGATG	7 cut(s)	962, 983, 1028, 1088, 1804, 1838, 2189
FriOI	GRGCYC	3 cut(s)	546, 731, 2441
FspBI	CTAG	7 cut(s)	518, 1095, 1113, 1260, 2355, 2558, 2865
GlaI	GCGC	6 cut(s)	151, 163, 486, 853, 1099, 1245
GsaI	CCCAGC	1 cut(s)	1126
GsuI	CTGGAG	3 cut(s)	2105, 2192, 2786
HaeII	RGCGCY	4 cut(s)	165, 488, 855, 1101
HaeIII	GGCC	4 cut(s)	564, 692, 701, 1186
HapII	CCGG	2 cut(s)	610, 815
HgaI	GACGC	3 cut(s)	188, 417, 1515
HhaI	GCGC	6 cut(s)	152, 164, 487, 854, 1100, 1246
Hin1I	GRCGYC	1 cut(s)	162
Hin6I	GCGC	6 cut(s)	150, 162, 485, 852, 1098, 1244
HinP1I	GCGC	6 cut(s)	150, 162, 485, 852, 1098, 1244
HincII	GTYRAC	2 cut(s)	289, 390
HindII	GTYRAC	2 cut(s)	289, 390
HindIII	AAGCTT	5 cut(s)	272, 1266, 1289, 1424, 2564
HinfI	GANTC	9 cut(s)	581, 620, 626, 833, 1802, 1859, 1888, 2490, 2761
HpaII	CCGG	2 cut(s)	610, 815
HphI	GGTGA	3 cut(s)	723, 1016, 2252
Hpy166II	GTNNAC	7 cut(s)	289, 390, 432, 1897, 2168, 2272, 2810
Hpy188I	TCNGA	7 cut(s)	541, 558, 592, 625, 631, 2197, 2847
Hpy8I	GTNNAC	7 cut(s)	289, 390, 432, 1897, 2168, 2272, 2810
Hpy99I	CGWCG	6 cut(s)	158, 161, 179, 182, 296, 635
HpyAV	CCTTC	9 cut(s)	381, 543, 553, 640, 854, 1613, 1847, 2361, 2527
HpyCH4III	ACNGT	8 cut(s)	407, 926, 1797, 1815, 1883, 2030, 2276, 2425
HpyCH4IV	ACGT	4 cut(s)	450, 1522, 1747, 2343
HpyF3I	CTNAG	6 cut(s)	278, 599, 791, 1329, 1806, 2288
HpySE526I	ACGT	4 cut(s)	450, 1522, 1747, 2343
Hsp92I	GRCGYC	1 cut(s)	162
HspAI	GCGC	6 cut(s)	150, 162, 485, 852, 1098, 1244
KasI	GGCGCC	1 cut(s)	161
KflI	GGGWCCC	1 cut(s)	2101
KpnI	GGTACC	1 cut(s)	471
Ksp22I	TGATCA	1 cut(s)	748
Kzo9I	GATC	5 cut(s)	128, 166, 748, 2224, 2842
LguI	GCTCTTC	2 cut(s)	1035, 1479
LmnI	GCTCC	9 cut(s)	326, 492, 530, 1105, 1208, 1477, 2086, 2436, 2578
MabI	ACCWGGT	1 cut(s)	733
MaeI	CTAG	7 cut(s)	518, 1095, 1113, 1260, 2355, 2558, 2865
MaeII	ACGT	4 cut(s)	450, 1522, 1747, 2343
MaeIII	GTNAC	8 cut(s)	1147, 1320, 1451, 1877, 2332, 2410, 2641, 2788
MalI	GATC	5 cut(s)	130, 168, 750, 2226, 2844
MbiI	CCGCTC	1 cut(s)	321
MboI	GATC	5 cut(s)	128, 166, 748, 2224, 2842
MfeI	CAATTG	1 cut(s)	1203
MflI	RGATCY	1 cut(s)	128
MhlI	GDGCHC	6 cut(s)	546, 731, 1122, 1923, 2441, 2748
MluCI	AATT	8 cut(s)	711, 768, 897, 1203, 1279, 1342, 1638, 1718
Mly113I	GGCGCC	1 cut(s)	162
MlyI	GAGTC	5 cut(s)	575, 614, 1796, 1897, 2755
MmeI	TCCRAC	4 cut(s)	471, 597, 654, 2258
Mph1103I	ATGCAT	2 cut(s)	6, 2020
MseI	TTAA	9 cut(s)	204, 666, 1293, 1467, 1740, 2048, 2153, 2825, 2892
MslI	CAYNNNNRTG	2 cut(s)	437, 1826
MspA1I	CMGCKG	2 cut(s)	1586, 1646
MspCI	CTTAAG	3 cut(s)	1292, 1739, 2891
MspI	CCGG	2 cut(s)	610, 815
MspR9I	CCNGG	6 cut(s)	73, 610, 611, 735, 815, 2140
MunI	CAATTG	1 cut(s)	1203
Mva1269I	GAATGC	4 cut(s)	91, 2143, 2314, 2776
MvaI	CCWGG	3 cut(s)	73, 735, 2140
MvnI	CGCG	6 cut(s)	9, 150, 342, 428, 511, 1431
NarI	GGCGCC	1 cut(s)	162
NciI	CCSGG	3 cut(s)	610, 611, 815
NcoI	CCATGG	1 cut(s)	1821
NdeI	CATATG	2 cut(s)	1448, 2074
NdeII	GATC	5 cut(s)	128, 166, 748, 2224, 2842
NlaIV	GGNNCC	9 cut(s)	163, 469, 879, 1110, 2102, 2103, 2427, 2438, 2708
NmeAIII	GCCGAG	1 cut(s)	342
NmuCI	GTSAC	6 cut(s)	1147, 1320, 1451, 1877, 2410, 2788
NruI	TCGCGA	1 cut(s)	1431
NsiI	ATGCAT	2 cut(s)	6, 2020
NspV	TTCGAA	1 cut(s)	1223
OliI	CACNNNNGTG	1 cut(s)	437
PaeR7I	CTCGAG	1 cut(s)	1376
PagI	TCATGA	2 cut(s)	1284, 1513
PciSI	GCTCTTC	2 cut(s)	1035, 1479
PcsI	WCGNNNNNNNCGW	4 cut(s)	13, 162, 291, 425
PctI	GAATGC	4 cut(s)	91, 2143, 2314, 2776
PfeI	GAWTC	4 cut(s)	626, 833, 1859, 2490
PleI	GAGTC	5 cut(s)	575, 614, 1796, 1896, 2755
PluTI	GGCGCC	1 cut(s)	165
PpsI	GAGTC	5 cut(s)	575, 614, 1796, 1896, 2755
PpuMI	RGGWCCY	1 cut(s)	2101
Psp124BI	GAGCTC	1 cut(s)	731
Psp1406I	AACGTT	1 cut(s)	450
Psp5II	RGGWCCY	1 cut(s)	2101
Psp6I	CCWGG	3 cut(s)	71, 733, 2138
PspFI	CCCAGC	1 cut(s)	1122
PspGI	CCWGG	3 cut(s)	71, 733, 2138
PspN4I	GGNNCC	9 cut(s)	163, 469, 879, 1110, 2102, 2103, 2427, 2438, 2708
PspPI	GGNCC	7 cut(s)	403, 562, 691, 1184, 2101, 2425, 2707
PspPPI	RGGWCCY	1 cut(s)	2101
PstI	CTGCAG	3 cut(s)	501, 1147, 1933
PstNI	CAGNNNCTG	1 cut(s)	2318
PsuI	RGATCY	1 cut(s)	128
PvuII	CAGCTG	2 cut(s)	1586, 1646
RruI	TCGCGA	1 cut(s)	1431
RsaI	GTAC	5 cut(s)	469, 1093, 1409, 1794, 1799
RsaNI	GTAC	5 cut(s)	468, 1092, 1408, 1793, 1798
RseI	CAYNNNNRTG	2 cut(s)	437, 1826
SacI	GAGCTC	1 cut(s)	731
SapI	GCTCTTC	2 cut(s)	1035, 1479
SaqAI	TTAA	9 cut(s)	204, 666, 1293, 1467, 1740, 2048, 2153, 2825, 2892
Sau3AI	GATC	5 cut(s)	128, 166, 748, 2224, 2842
Sau96I	GGNCC	7 cut(s)	403, 562, 691, 1184, 2101, 2425, 2707
ScaI	AGTACT	1 cut(s)	1093
SchI	GAGTC	5 cut(s)	575, 614, 1796, 1897, 2755
ScrFI	CCNGG	6 cut(s)	73, 610, 611, 735, 815, 2140
SduI	GDGCHC	6 cut(s)	546, 731, 1122, 1923, 2441, 2748
SexAI	ACCWGGT	1 cut(s)	733
SfcI	CTRYAG	3 cut(s)	497, 1143, 1929
SfiI	GGCCNNNNNGGCC	1 cut(s)	698
SfoI	GGCGCC	1 cut(s)	163
Sfr274I	CTCGAG	1 cut(s)	1376
SfuI	TTCGAA	1 cut(s)	1223
SinI	GGWCC	4 cut(s)	403, 2101, 2425, 2707
SlaI	CTCGAG	1 cut(s)	1376
SmaI	CCCGGG	1 cut(s)	611
SmiMI	CAYNNNNRTG	2 cut(s)	437, 1826
SmlI	CTYRAG	5 cut(s)	398, 1292, 1376, 1739, 2891
SmoI	CTYRAG	5 cut(s)	398, 1292, 1376, 1739, 2891
Sse9I	AATT	8 cut(s)	711, 768, 897, 1203, 1279, 1342, 1638, 1718
SspDI	GGCGCC	1 cut(s)	161
SspI	AATATT	2 cut(s)	1366, 1964
SspMI	CTAG	7 cut(s)	518, 1095, 1113, 1260, 2355, 2558, 2865
SstI	GAGCTC	1 cut(s)	731
StyD4I	CCNGG	6 cut(s)	71, 608, 609, 733, 813, 2138
StyI	CCWWGG	1 cut(s)	1821
TaaI	ACNGT	8 cut(s)	407, 926, 1797, 1815, 1883, 2030, 2276, 2425
TaiI	ACGT	4 cut(s)	453, 1525, 1750, 2346
TasI	AATT	8 cut(s)	711, 768, 897, 1203, 1279, 1342, 1638, 1718
TatI	WGTACW	1 cut(s)	1091
TauI	GCSGC	6 cut(s)	147, 150, 311, 2083, 2471, 2624
TfiI	GAWTC	4 cut(s)	626, 833, 1859, 2490
Tru1I	TTAA	9 cut(s)	204, 666, 1293, 1467, 1740, 2048, 2153, 2825, 2892
Tru9I	TTAA	9 cut(s)	204, 666, 1293, 1467, 1740, 2048, 2153, 2825, 2892
TseFI	GTSAC	6 cut(s)	1147, 1320, 1451, 1877, 2410, 2788
Tsp45I	GTSAC	6 cut(s)	1147, 1320, 1451, 1877, 2410, 2788
TspGWI	ACGGA	5 cut(s)	46, 427, 479, 593, 2099
TspMI	CCCGGG	1 cut(s)	609
Vha464I	CTTAAG	3 cut(s)	1292, 1739, 2891
VpaK11BI	GGWCC	4 cut(s)	403, 2101, 2425, 2707
XapI	RAATTY	3 cut(s)	1279, 1342, 1718
XbaI	TCTAGA	1 cut(s)	2557
XhoI	CTCGAG	1 cut(s)	1376
XmaI	CCCGGG	1 cut(s)	609
XmiI	GTMKAC	1 cut(s)	1896
XspI	CTAG	7 cut(s)	518, 1095, 1113, 1260, 2355, 2558, 2865
ZrmI	AGTACT	1 cut(s)	1093
Zsp2I	ATGCAT	2 cut(s)	6, 2020

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.