Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0004762

Physical Location & Seq

Genomic Coordinates Forward (+)

6022898 .. 6024003

Gene Structure 1106 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1086600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 714 bp

ATGGCGCCAAAGGCAACCAGAAATCAAAGTCCGCCGTCCGATCCTCAGGGAATGTTCGCCGGAATGGTCGTCTTCTTAGTAGAAAACGGCGTTCAGAGTCGCCGTTTACAGATTTGGAAGCAAAAATTGGTCCAAATGGGGGCCTCCATTGAGGAACAGTTAACCAAAAAGGTCACTCACATACTTGCTGTGAGCCCAAAAGCCCTCTTAGAACGAGTGGGCAGTGAGAAATTGGCTAATTTCAAAGGAAGTGTTTTGGTTTATCAGTGGCTTGAGGACAGCTTGAGCTCAGGAAAATTGGTGTCTGAGGATTTGTACCCTCTAAAATTGGAAGCAGAAAAGTCTTCCAGTGATTATGTTTCGAGCGATGACGAAAAACCAAAACCGAAAAAGAGAAGATCATCTTCTCCTGGTGATGAGTTGGTTCAGACCTCAATACCCAAAACCCACGAAGCCCCAAATAAGGATCAGGTTGCAGCATTGGAGTCAACCGTGCCCTACAGCCCACCTGATTTAAATAGGAACATAACCGAGATTTTCGGGAAACTTATCAATATATATAGAGCCTTGGGTGATGACCGAAGATCATTTAGCTATTACAAGGCTATAGCGGTTATAGAGAAGCTACCTTTCAAGATTCAAAGTATGGACCAAGTTAAAGACCTACCCAACATTGGAAAGTCAATGCAAGATCATGTAAGTCAATGTGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0000726 GO:0003674 GO:0003824 GO:0003887 GO:0005488 GO:0006139 GO:0006259 GO:0006281 GO:0006284 GO:0006285 GO:0006287 GO:0006289 GO:0006302 GO:0006303 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0010224 GO:0016740 GO:0016772 GO:0016779 GO:0016829 GO:0016835 GO:0018130 GO:0019438 GO:0030145 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0043167 GO:0043169 GO:0043170 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0046483 GO:0046872 GO:0046914 GO:0050896 GO:0051575 GO:0051716 GO:0071704 GO:0071897 GO:0090304 GO:0097510 GO:0140097 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03410 ko03450 map03410 map03450

Pfam Domains

Protein Families

BRCT DNA_pol_B_palm DNA_pol_B_thumb DNA_pol_lambd_f HHH_8 LIM

Protein Analysis

238

Amino Acids

26.42

Weight (kDa)

8.96

Isoelectric Point (pI)

65.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BRCT	PF00533	17 - 94	2.2e-06	BRCA1 C Terminus (BRCT) domain
HHH_8	PF14716	173 - 234	1.2e-10	Helix-hairpin-helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004762)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10520 AT1G10520
fragaria_vesca	FvH4_4g35010 FvH4_4g35030
malus_domestica	MD16G1086600.v1.1 MD16G1086700.v1.1
prunus_persica	Prupe.1G259900_v2.0.a1 Prupe.1G259900_v2.0.a1 Prupe.1G259900_v2.0.a1 Prupe.1G259900_v2.0.a1
pyrus_communis	pycom16g07470
rosa_chinensis	RchiOBHm_Chr4g0444541 RchiOBHm_Chr4g0444551
rosa_laevigata	RLG00000005822 RLG00000005825
rosa_multiflora	Rmu_sc0003839.1_g000005 Rmu_sc0012648.1_g000007
rosa_roxburghii	Rroxscaffold_5G00385000 Rroxscaffold_5G00385010
rosa_rugosa	Rorug04G0353500 Rorug04G0353600 Rorug04G0353700 Rorug04G0353800
rosa_samantha	Rh4AG413800 Rh4BG425500 Rh4CG439900 Rh4CG440200 Rh4DG420800 Rh4DG421100
rosa_wichuraiana	Rw4G035540 Rw4G035550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	4
AciI	CCGC	2 cut(s)	32, 611
AclWI	GGATC	2 cut(s)	35, 474
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	1 cut(s)	317
AfiI	CCNNNNNNNGG	3 cut(s)	139, 463, 674
AgsI	TTSAA	3 cut(s)	244, 634, 641
AjnI	CCWGG	1 cut(s)	409
AjuI	GAANNNNNNNTTGG	2 cut(s)	110, 142
AluBI	AGCT	4 cut(s)	282, 288, 594, 625
AluI	AGCT	4 cut(s)	282, 288, 594, 625
Alw21I	GWGCWC	1 cut(s)	290
AlwI	GGATC	2 cut(s)	35, 474
AoxI	GGCC	1 cut(s)	141
ApeKI	GCWGC	1 cut(s)	476
AspLEI	GCGC	1 cut(s)	7
AspS9I	GGNCC	3 cut(s)	130, 141, 649
AsuHPI	GGTGA	2 cut(s)	425, 584
AvaII	GGWCC	2 cut(s)	130, 649
AxyI	CCTNAGG	1 cut(s)	45
BaeGI	GKGCMC	1 cut(s)	498
BanI	GGYRCC	1 cut(s)	4
BanII	GRGCYC	2 cut(s)	197, 290
BbsI	GAAGAC	2 cut(s)	64, 336
Bbv12I	GWGCWC	1 cut(s)	290
BbvI	GCAGC	1 cut(s)	488
BceAI	ACGGC	3 cut(s)	19, 87, 103
BciT130I	CCWGG	1 cut(s)	411
BfmI	CTRYAG	2 cut(s)	499, 606
BfoI	RGCGCY	1 cut(s)	8
BisI	GCNGC	1 cut(s)	477
BlsI	GCNGC	1 cut(s)	478
Bme1390I	CCNGG	1 cut(s)	411
Bme18I	GGWCC	2 cut(s)	130, 649
BmgT120I	GGNCC	3 cut(s)	130, 141, 649
BmiI	GGNNCC	2 cut(s)	6, 142
BmrFI	CCNGG	1 cut(s)	411
BpiI	GAAGAC	2 cut(s)	64, 336
Bpu10I	CCTNAGC	1 cut(s)	289
BpuEI	CTTGAG	2 cut(s)	293, 304
BsaHI	GRCGYC	1 cut(s)	5
BsaJI	CCNNGG	1 cut(s)	567
Bsc4I	CCNNNNNNNGG	3 cut(s)	139, 463, 674
Bse1I	ACTGG	1 cut(s)	348
Bse21I	CCTNAGG	1 cut(s)	45
BseBI	CCWGG	1 cut(s)	411
BseDI	CCNNGG	1 cut(s)	567
BseLI	CCNNNNNNNGG	3 cut(s)	139, 463, 674
BseMII	CTCAG	3 cut(s)	59, 297, 303
BseNI	ACTGG	1 cut(s)	348
BseSI	GKGCMC	1 cut(s)	498
BseXI	GCAGC	1 cut(s)	488
BshFI	GGCC	1 cut(s)	143
BshNI	GGYRCC	1 cut(s)	4
BsiHKAI	GWGCWC	1 cut(s)	290
BsiSI	CCGG	1 cut(s)	60
BslI	CCNNNNNNNGG	3 cut(s)	139, 463, 674
BsnI	GGCC	1 cut(s)	143
Bsp1286I	GDGCHC	3 cut(s)	197, 290, 498
Bsp143I	GATC	5 cut(s)	40, 398, 466, 584, 691
BspACI	CCGC	2 cut(s)	32, 611
BspANI	GGCC	1 cut(s)	143
BspCNI	CTCAG	3 cut(s)	58, 298, 302
BspLI	GGNNCC	2 cut(s)	6, 142
BspPI	GGATC	2 cut(s)	35, 474
BspT107I	GGYRCC	1 cut(s)	4
BsrI	ACTGG	1 cut(s)	348
BssECI	CCNNGG	1 cut(s)	567
BssMI	GATC	5 cut(s)	40, 398, 466, 584, 691
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	1 cut(s)	567
Bst2UI	CCWGG	1 cut(s)	411
Bst4CI	ACNGT	2 cut(s)	159, 493
BstACI	GRCGYC	1 cut(s)	5
BstDEI	CTNAG	5 cut(s)	45, 76, 208, 289, 306
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	1 cut(s)	7
BstKTI	GATC	5 cut(s)	43, 401, 469, 587, 694
BstMBI	GATC	5 cut(s)	40, 398, 466, 584, 691
BstMWI	GCNNNNNNNGC	1 cut(s)	11
BstNI	CCWGG	1 cut(s)	411
BstSCI	CCNGG	1 cut(s)	409
BstSFI	CTRYAG	2 cut(s)	499, 606
BstSLI	GKGCMC	1 cut(s)	498
BstV1I	GCAGC	1 cut(s)	488
BstV2I	GAAGAC	2 cut(s)	64, 336
Bsu36I	CCTNAGG	1 cut(s)	45
BsuRI	GGCC	1 cut(s)	143
BtgZI	GCGATG	1 cut(s)	381
BtsI	GCAGTG	1 cut(s)	229
BtsIMutI	CAGTG	3 cut(s)	229, 272, 355
CfoI	GCGC	1 cut(s)	7
Cfr13I	GGNCC	3 cut(s)	130, 141, 649
Csp6I	GTAC	1 cut(s)	316
CviAII	CATG	2 cut(s)	695, 711
CviQI	GTAC	1 cut(s)	316
DdeI	CTNAG	5 cut(s)	45, 76, 208, 289, 306
DinI	GGCGCC	1 cut(s)	6
DpnI	GATC	5 cut(s)	42, 400, 468, 586, 693
DpnII	GATC	5 cut(s)	40, 398, 466, 584, 691
DraI	TTTAAA	1 cut(s)	516
EciI	GGCGGA	1 cut(s)	21
Ecl136II	GAGCTC	1 cut(s)	288
Eco130I	CCWWGG	1 cut(s)	567
Eco24I	GRGCYC	2 cut(s)	197, 290
Eco47I	GGWCC	2 cut(s)	130, 649
Eco53kI	GAGCTC	1 cut(s)	288
Eco81I	CCTNAGG	1 cut(s)	45
EcoICRI	GAGCTC	1 cut(s)	288
EcoO109I	RGGNCCY	1 cut(s)	141
EcoRII	CCWGG	1 cut(s)	409
EcoT14I	CCWWGG	1 cut(s)	567
EcoT38I	GRGCYC	2 cut(s)	197, 290
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
ErhI	CCWWGG	1 cut(s)	567
FaeI	CATG	2 cut(s)	698, 714
FalI	AAGNNNNNCTT	2 cut(s)	388, 420
FatI	CATG	2 cut(s)	694, 710
Fnu4HI	GCNGC	1 cut(s)	477
FriOI	GRGCYC	2 cut(s)	197, 290
Fsp4HI	GCNGC	1 cut(s)	477
GlaI	GCGC	1 cut(s)	6
GluI	GCNGC	1 cut(s)	477
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	1 cut(s)	143
HapII	CCGG	1 cut(s)	60
HhaI	GCGC	1 cut(s)	7
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	2 cut(s)	698, 714
Hin6I	GCGC	1 cut(s)	5
HinP1I	GCGC	1 cut(s)	5
HincII	GTYRAC	2 cut(s)	162, 489
HindII	GTYRAC	2 cut(s)	162, 489
HinfI	GANTC	3 cut(s)	97, 485, 637
HpaI	GTTAAC	1 cut(s)	162
HpaII	CCGG	1 cut(s)	60
HphI	GGTGA	2 cut(s)	425, 584
Hpy166II	GTNNAC	3 cut(s)	107, 162, 489
Hpy188I	TCNGA	4 cut(s)	40, 96, 307, 429
Hpy188III	TCNNGA	3 cut(s)	291, 541, 634
Hpy8I	GTNNAC	3 cut(s)	107, 162, 489
HpyCH4III	ACNGT	2 cut(s)	159, 493
HpyCH4V	TGCA	3 cut(s)	476, 688, 710
HpyF10VI	GCNNNNNNNGC	1 cut(s)	11
HpyF3I	CTNAG	5 cut(s)	45, 76, 208, 289, 306
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	2 cut(s)	698, 714
HspAI	GCGC	1 cut(s)	5
KasI	GGCGCC	1 cut(s)	4
KspAI	GTTAAC	1 cut(s)	162
Kzo9I	GATC	5 cut(s)	40, 398, 466, 584, 691
LpnPI	CCDG	9 cut(s)	31, 32, 73, 276, 361, 396, 423, 455, 522
Lsp1109I	GCAGC	1 cut(s)	488
MaeIII	GTNAC	1 cut(s)	172
MalI	GATC	5 cut(s)	42, 400, 468, 586, 693
MboI	GATC	5 cut(s)	40, 398, 466, 584, 691
MboII	GAAGA	5 cut(s)	64, 336, 396, 408, 594
MhlI	GDGCHC	3 cut(s)	197, 290, 498
MluCI	AATT	5 cut(s)	125, 230, 238, 296, 326
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	2 cut(s)	106, 494
MnlI	CCTC	8 cut(s)	54, 145, 154, 215, 268, 301, 330, 442
MseI	TTAA	3 cut(s)	161, 515, 657
MspI	CCGG	1 cut(s)	60
MspR9I	CCNGG	1 cut(s)	411
MvaI	CCWGG	1 cut(s)	411
MwoI	GCNNNNNNNGC	1 cut(s)	11
NarI	GGCGCC	1 cut(s)	5
NdeII	GATC	5 cut(s)	40, 398, 466, 584, 691
NlaIII	CATG	2 cut(s)	698, 714
NlaIV	GGNNCC	2 cut(s)	6, 142
NmuCI	GTSAC	1 cut(s)	172
PfeI	GAWTC	1 cut(s)	637
PkrI	GCNGC	1 cut(s)	478
PleI	GAGTC	2 cut(s)	105, 493
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	2 cut(s)	105, 493
Psp124BI	GAGCTC	1 cut(s)	290
Psp6I	CCWGG	1 cut(s)	409
PspGI	CCWGG	1 cut(s)	409
PspN4I	GGNNCC	2 cut(s)	6, 142
PspPI	GGNCC	3 cut(s)	130, 141, 649
RsaI	GTAC	1 cut(s)	317
RsaNI	GTAC	1 cut(s)	316
SacI	GAGCTC	1 cut(s)	290
SaqAI	TTAA	3 cut(s)	161, 515, 657
SatI	GCNGC	1 cut(s)	477
Sau3AI	GATC	5 cut(s)	40, 398, 466, 584, 691
Sau96I	GGNCC	3 cut(s)	130, 141, 649
SchI	GAGTC	2 cut(s)	106, 494
ScrFI	CCNGG	1 cut(s)	411
SduI	GDGCHC	3 cut(s)	197, 290, 498
SfcI	CTRYAG	2 cut(s)	499, 606
SfoI	GGCGCC	1 cut(s)	6
SinI	GGWCC	2 cut(s)	130, 649
SmiI	ATTTAAAT	1 cut(s)	516
SmlI	CTYRAG	2 cut(s)	272, 283
SmoI	CTYRAG	2 cut(s)	272, 283
Sse9I	AATT	5 cut(s)	125, 230, 238, 296, 326
SsiI	CCGC	2 cut(s)	32, 611
SspDI	GGCGCC	1 cut(s)	4
SstI	GAGCTC	1 cut(s)	290
StyD4I	CCNGG	1 cut(s)	409
StyI	CCWWGG	1 cut(s)	567
SwaI	ATTTAAAT	1 cut(s)	516
TaaI	ACNGT	2 cut(s)	159, 493
TaqI	TCGA	1 cut(s)	362
TaqII	GACCGA	1 cut(s)	594
TasI	AATT	5 cut(s)	125, 230, 238, 296, 326
TfiI	GAWTC	1 cut(s)	637
Tru1I	TTAA	3 cut(s)	161, 515, 657
Tru9I	TTAA	3 cut(s)	161, 515, 657
TscAI	CASTG	3 cut(s)	229, 272, 355
TseFI	GTSAC	1 cut(s)	172
TseI	GCWGC	1 cut(s)	476
Tsp45I	GTSAC	1 cut(s)	172
TspRI	CASTG	3 cut(s)	229, 272, 355
VpaK11BI	GGWCC	2 cut(s)	130, 649

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.