Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0004762

Physical Location & Seq

Genomic Coordinates Reverse (-)

62888491 .. 62892740

Gene Structure 4250 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG421100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1230 bp

ATGCTCTGGTGTGTTCCTAAGACACGCGTCCTCCACCGATCAGGATTGCTGATCAGAAGTAAAACAGTAAAAATGACTTCGCCTTTGTTGCACAACCGAAACCAAGTAATGGCGCCAAAAAAGGCAACCAGAGATGAAAGTCCTCCACCGTCCGATCCTCACGGAATCTTCGCCGGAATGGTCGTCTTCTTAGTCGAAGACGGAGTTCAGAACCGTCGCTTACAGGTTTGGAAGCAAAAACTGGTTCAAATGGGAGCTACTATAGAAGACCATCTATCCAAAAGGGTCACACACATATTTGCTATGAACCCAGAAGCCCTTTTGCAAAAAGTGGGCAGTGATCAACGGGGTCTCTTTAAAGGAAGAGTTGTACTCTATCAGTGGCTAGAGGACAGCTTGAGCTCAGGGAAGAGAGTACCAGAAGATTTGTACCATCTGAAATTGGAGTCAGATAAGTCTTCTAGTGATTACATTTCCAGTGATGAAGAAACCCCAAAACTGAAAAGGCGAAGATCCTCATCTCCTGATGAGTTGCCTCATACTCCCAGCCCCAAAACCCCTAAAGCCCCAAATAAGGATCAGGTCACAGCATTGCAGTCATCCACGCCCTACAACCCACCTGATTTAAACAAAGACATAACCCAGATATTTGGGAAGCTTATCAACATATATAGAGCATTGGGCGATGACCGAAGATCTTTTAGCTACTACAAGGCTATAACAGTTATTGAGAAGTTGCCTTTCAAGATTCAAAGTGCAGAACAAGTTAAGGGCCTCCCGGGCATTGGAAAGTCAATGCAAGATCATGTGGTTGTTGTATGTGGAGGGTCATATAGACGCGGGAAATCTTCTTGTGGGGATCTGGACATTGTAATTACTCATCCAGATGGGAACAGGCATAAAGGTTTTTTACTGAAGTATGTAAAGCATCTAAAGGATATGAAGTTCCTAAGGGAGGATTTGGTTTTCGTTACACACAGCGAGGAGGGTACTGATTCTGGTGTCGACACATATTATGGGCTTTGTACATATCCTGGACGAGAGCTGCGGCACCGCATAGATTTCAAGGTCTACCCGCGGGACATATATGCATTTGGACTAATAGCTTGGACAGGGAATGATGTGCTGAATAGGAGGTTGAGGTTACTAGCTGAATCTAAAGGATTCCGGCTTGACGATACAGGGCTGTTTCCGGCCACTCATGGATCTGGCGGAAAACGGTTAATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0000726 GO:0003674 GO:0003824 GO:0003887 GO:0005488 GO:0006139 GO:0006259 GO:0006281 GO:0006284 GO:0006285 GO:0006287 GO:0006289 GO:0006302 GO:0006303 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0010224 GO:0016740 GO:0016772 GO:0016779 GO:0016829 GO:0016835 GO:0018130 GO:0019438 GO:0030145 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0043167 GO:0043169 GO:0043170 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0046483 GO:0046872 GO:0046914 GO:0050896 GO:0051575 GO:0051716 GO:0071704 GO:0071897 GO:0090304 GO:0097510 GO:0140097 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03410 ko03450 map03410 map03450

Pfam Domains

Protein Families

BRCT DNA_pol_B_palm DNA_pol_B_thumb DNA_pol_lambd_f HHH_8 LIM

Protein Analysis

409

Amino Acids

46.26

Weight (kDa)

9.6

Isoelectric Point (pI)

54.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HHH_8	PF14716	210 - 270	1.7e-11	Helix-hairpin-helix domain
DNA_pol_B_palm	PF14792	264 - 360	1.3e-22	DNA polymerase beta palm
DNA_pol_B_thumb	PF14791	367 - 403	2.6e-08	DNA polymerase beta thumb

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004762)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10520 AT1G10520
fragaria_vesca	FvH4_4g35010 FvH4_4g35030
malus_domestica	MD16G1086600.v1.1 MD16G1086700.v1.1
prunus_persica	Prupe.1G259900_v2.0.a1 Prupe.1G259900_v2.0.a1 Prupe.1G259900_v2.0.a1 Prupe.1G259900_v2.0.a1
pyrus_communis	pycom16g07470
rosa_chinensis	RchiOBHm_Chr4g0444541 RchiOBHm_Chr4g0444551
rosa_laevigata	RLG00000005822 RLG00000005825
rosa_multiflora	Rmu_sc0003839.1_g000005 Rmu_sc0012648.1_g000007
rosa_roxburghii	Rroxscaffold_5G00385000 Rroxscaffold_5G00385010
rosa_rugosa	Rorug04G0353500 Rorug04G0353600 Rorug04G0353700 Rorug04G0353800
rosa_samantha	Rh4AG413800 Rh4BG425500 Rh4CG439900 Rh4CG440200 Rh4DG420800 Rh4DG421100
rosa_wichuraiana	Rw4G035540 Rw4G035550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	112, 1050
AccB7I	CCANNNNNTGG	1 cut(s)	109
AccI	GTMKAC	2 cut(s)	1005, 1071
AccII	CGCG	3 cut(s)	27, 840, 1078
AciI	CCGC	6 cut(s)	840, 1048, 1054, 1076, 1078, 1212
AclWI	GGATC	5 cut(s)	149, 507, 585, 867, 1213
AcoI	YGGCCR	1 cut(s)	1194
AcuI	CTGAAG	1 cut(s)	935
AcyI	GRCGYC	1 cut(s)	113
AfaI	GTAC	5 cut(s)	372, 417, 431, 991, 1027
AfiI	CCNNNNNNNGG	4 cut(s)	109, 574, 785, 955
AflIII	ACRYGT	1 cut(s)	25
AgsI	TTSAA	4 cut(s)	248, 745, 752, 1066
AjnI	CCWGG	1 cut(s)	1033
AloI	GAACNNNNNNTCC	2 cut(s)	929, 961
AluBI	AGCT	8 cut(s)	257, 396, 402, 658, 705, 1045, 1106, 1151
AluI	AGCT	8 cut(s)	257, 396, 402, 658, 705, 1045, 1106, 1151
Alw21I	GWGCWC	1 cut(s)	404
Alw26I	GTCTC	1 cut(s)	356
AlwI	GGATC	5 cut(s)	149, 507, 585, 867, 1213
Ama87I	CYCGRG	1 cut(s)	778
AoxI	GGCC	2 cut(s)	772, 1194
ApeKI	GCWGC	1 cut(s)	1045
ArsI	GACNNNNNNTTYG	2 cut(s)	67, 99
AspLEI	GCGC	1 cut(s)	115
AspS9I	GGNCC	1 cut(s)	772
AsuC2I	CCSGG	2 cut(s)	779, 780
AvaI	CYCGRG	1 cut(s)	778
AxyI	CCTNAGG	1 cut(s)	950
BanI	GGYRCC	2 cut(s)	112, 1050
BanII	GRGCYC	1 cut(s)	404
BbsI	GAAGAC	4 cut(s)	178, 204, 273, 450
Bbv12I	GWGCWC	1 cut(s)	404
BbvI	GCAGC	1 cut(s)	1032
BccI	CCATC	3 cut(s)	279, 441, 881
BciT130I	CCWGG	1 cut(s)	1035
BclI	TGATCA	2 cut(s)	51, 340
BcnI	CCSGG	2 cut(s)	779, 780
BcoDI	GTCTC	1 cut(s)	356
BfaI	CTAG	3 cut(s)	386, 462, 1148
BfmI	CTRYAG	1 cut(s)	261
BfoI	RGCGCY	1 cut(s)	116
BglI	GCCNNNNNGGC	1 cut(s)	780
BglII	AGATCT	1 cut(s)	695
BisI	GCNGC	2 cut(s)	1046, 1049
BlsI	GCNGC	2 cut(s)	1047, 1050
Bme1390I	CCNGG	3 cut(s)	779, 780, 1035
BmeT110I	CYCGRG	1 cut(s)	778
BmgT120I	GGNCC	1 cut(s)	772
BmiI	GGNNCC	2 cut(s)	114, 1052
BmrFI	CCNGG	3 cut(s)	779, 780, 1035
BmsI	GCATC	1 cut(s)	937
BoxI	GACNNNNGTC	1 cut(s)	26
BpiI	GAAGAC	4 cut(s)	178, 204, 273, 450
BplI	GAGNNNNNCTC	2 cut(s)	357, 389
Bpu10I	CCTNAGC	1 cut(s)	403
BpuEI	CTTGAG	1 cut(s)	418
BpuMI	CCSGG	2 cut(s)	779, 780
BsaBI	GATNNNNATC	1 cut(s)	517
BsaHI	GRCGYC	1 cut(s)	113
BsaI	GGTCTC	1 cut(s)	356
BsaJI	CCNNGG	2 cut(s)	778, 1076
BsaXI	ACNNNNNCTCC	2 cut(s)	15, 45
Bsc4I	CCNNNNNNNGG	4 cut(s)	109, 574, 785, 955
Bse1I	ACTGG	2 cut(s)	246, 477
Bse21I	CCTNAGG	1 cut(s)	950
Bse3DI	GCAATG	1 cut(s)	590
Bse8I	GATNNNNATC	1 cut(s)	517
BseBI	CCWGG	1 cut(s)	1035
BseDI	CCNNGG	2 cut(s)	778, 1076
BseGI	GGATG	2 cut(s)	599, 880
BseJI	GATNNNNATC	1 cut(s)	517
BseLI	CCNNNNNNNGG	4 cut(s)	109, 574, 785, 955
BseMI	GCAATG	1 cut(s)	590
BseMII	CTCAG	1 cut(s)	417
BseNI	ACTGG	2 cut(s)	246, 477
BseRI	GAGGAG	1 cut(s)	998
BseXI	GCAGC	1 cut(s)	1032
BseYI	CCCAGC	1 cut(s)	545
BsgI	GTGCAG	1 cut(s)	777
Bsh1236I	CGCG	3 cut(s)	27, 840, 1078
BshFI	GGCC	2 cut(s)	774, 1196
BshNI	GGYRCC	2 cut(s)	112, 1050
BsiHKAI	GWGCWC	1 cut(s)	404
BsiHKCI	CYCGRG	1 cut(s)	778
BsiSI	CCGG	4 cut(s)	174, 779, 1168, 1193
BslFI	GGGAC	1 cut(s)	1094
BslI	CCNNNNNNNGG	4 cut(s)	109, 574, 785, 955
BsmAI	GTCTC	1 cut(s)	356
BsmFI	GGGAC	1 cut(s)	1094
BsnI	GGCC	2 cut(s)	774, 1196
Bso31I	GGTCTC	1 cut(s)	356
BsoBI	CYCGRG	1 cut(s)	778
Bsp1286I	GDGCHC	1 cut(s)	404
Bsp1407I	TGTACA	1 cut(s)	1025
BspACI	CCGC	6 cut(s)	840, 1048, 1054, 1076, 1078, 1212
BspANI	GGCC	2 cut(s)	774, 1196
BspCNI	CTCAG	1 cut(s)	416
BspFNI	CGCG	3 cut(s)	27, 840, 1078
BspLI	GGNNCC	2 cut(s)	114, 1052
BspPI	GGATC	5 cut(s)	149, 507, 585, 867, 1213
BspT107I	GGYRCC	2 cut(s)	112, 1050
BspTNI	GGTCTC	1 cut(s)	356
BsrDI	GCAATG	1 cut(s)	590
BsrGI	TGTACA	1 cut(s)	1025
BsrI	ACTGG	2 cut(s)	246, 477
BssECI	CCNNGG	2 cut(s)	778, 1076
BssNI	GRCGYC	1 cut(s)	113
Bst2UI	CCWGG	1 cut(s)	1035
Bst4CI	ACNGT	5 cut(s)	67, 150, 215, 724, 1221
Bst6I	CTCTTC	2 cut(s)	358, 404
BstACI	GRCGYC	1 cut(s)	113
BstAUI	TGTACA	1 cut(s)	1025
BstDEI	CTNAG	4 cut(s)	18, 190, 403, 950
BstDSI	CCRYGG	1 cut(s)	1076
BstENI	CCTNNNNNAGG	1 cut(s)	953
BstF5I	GGATG	2 cut(s)	599, 880
BstFNI	CGCG	3 cut(s)	27, 840, 1078
BstH2I	RGCGCY	1 cut(s)	116
BstHHI	GCGC	1 cut(s)	115
BstMAI	GTCTC	1 cut(s)	356
BstMWI	GCNNNNNNNGC	2 cut(s)	88, 780
BstNI	CCWGG	1 cut(s)	1035
BstPAI	GACNNNNGTC	1 cut(s)	26
BstSCI	CCNGG	3 cut(s)	777, 778, 1033
BstSFI	CTRYAG	1 cut(s)	261
BstUI	CGCG	3 cut(s)	27, 840, 1078
BstV1I	GCAGC	1 cut(s)	1032
BstV2I	GAAGAC	4 cut(s)	178, 204, 273, 450
BstX2I	RGATCY	4 cut(s)	512, 695, 859, 1205
BstXI	CCANNNNNNTGG	1 cut(s)	650
BstYI	RGATCY	4 cut(s)	512, 695, 859, 1205
Bsu36I	CCTNAGG	1 cut(s)	950
BsuRI	GGCC	2 cut(s)	774, 1196
BtgI	CCRYGG	1 cut(s)	1076
BtgZI	GCGATG	1 cut(s)	699
BtsCI	GGATG	2 cut(s)	599, 880
BtsI	GCAGTG	1 cut(s)	343
BtsIMutI	CAGTG	3 cut(s)	343, 386, 484
CfoI	GCGC	1 cut(s)	115
Cfr13I	GGNCC	1 cut(s)	772
Cfr42I	CCGCGG	1 cut(s)	1079
Cfr9I	CCCGGG	1 cut(s)	778
CseI	GACGC	2 cut(s)	16, 846
Csp6I	GTAC	5 cut(s)	371, 416, 430, 990, 1026
CviAII	CATG	2 cut(s)	806, 1202
CviQI	GTAC	5 cut(s)	371, 416, 430, 990, 1026
DdeI	CTNAG	4 cut(s)	18, 190, 403, 950
DinI	GGCGCC	1 cut(s)	114
DraI	TTTAAA	2 cut(s)	358, 627
EaeI	YGGCCR	1 cut(s)	1194
Eam1104I	CTCTTC	2 cut(s)	358, 404
EarI	CTCTTC	2 cut(s)	358, 404
EciI	GGCGGA	1 cut(s)	1227
Ecl136II	GAGCTC	1 cut(s)	402
Eco24I	GRGCYC	1 cut(s)	404
Eco31I	GGTCTC	1 cut(s)	356
Eco53kI	GAGCTC	1 cut(s)	402
Eco57I	CTGAAG	1 cut(s)	935
Eco81I	CCTNAGG	1 cut(s)	950
Eco88I	CYCGRG	1 cut(s)	778
EcoICRI	GAGCTC	1 cut(s)	402
EcoNI	CCTNNNNNAGG	1 cut(s)	953
EcoO109I	RGGNCCY	1 cut(s)	772
EcoRII	CCWGG	1 cut(s)	1033
EcoT22I	ATGCAT	1 cut(s)	1093
EcoT38I	GRGCYC	1 cut(s)	404
EgeI	GGCGCC	1 cut(s)	114
EheI	GGCGCC	1 cut(s)	114
FaeI	CATG	2 cut(s)	809, 1205
FaqI	GGGAC	1 cut(s)	1094
FatI	CATG	2 cut(s)	805, 1201
FauI	CCCGC	3 cut(s)	833, 1071, 1083
FbaI	TGATCA	2 cut(s)	51, 340
FblI	GTMKAC	2 cut(s)	1005, 1071
Fnu4HI	GCNGC	2 cut(s)	1046, 1049
FokI	GGATG	2 cut(s)	586, 867
FriOI	GRGCYC	1 cut(s)	404
Fsp4HI	GCNGC	2 cut(s)	1046, 1049
FspBI	CTAG	3 cut(s)	386, 462, 1148
GlaI	GCGC	1 cut(s)	114
GluI	GCNGC	2 cut(s)	1046, 1049
GsaI	CCCAGC	1 cut(s)	549
HaeII	RGCGCY	1 cut(s)	116
HaeIII	GGCC	2 cut(s)	774, 1196
HapII	CCGG	4 cut(s)	174, 779, 1168, 1193
HgaI	GACGC	2 cut(s)	16, 846
HhaI	GCGC	1 cut(s)	115
Hin1I	GRCGYC	1 cut(s)	113
Hin1II	CATG	2 cut(s)	809, 1205
Hin6I	GCGC	1 cut(s)	113
HinP1I	GCGC	1 cut(s)	113
HincII	GTYRAC	1 cut(s)	1006
HindII	GTYRAC	1 cut(s)	1006
HindIII	AAGCTT	1 cut(s)	656
HinfI	GANTC	6 cut(s)	165, 446, 748, 995, 1154, 1164
HpaII	CCGG	4 cut(s)	174, 779, 1168, 1193
Hpy166II	GTNNAC	2 cut(s)	1006, 1072
Hpy188I	TCNGA	5 cut(s)	56, 154, 210, 438, 451
Hpy188III	TCNNGA	5 cut(s)	42, 524, 745, 863, 884
Hpy8I	GTNNAC	2 cut(s)	1006, 1072
Hpy99I	CGWCG	1 cut(s)	219
HpyCH4III	ACNGT	5 cut(s)	67, 150, 215, 724, 1221
HpyCH4V	TGCA	6 cut(s)	91, 325, 595, 758, 799, 1091
HpyF10VI	GCNNNNNNNGC	2 cut(s)	88, 780
HpyF3I	CTNAG	4 cut(s)	18, 190, 403, 950
Hsp92I	GRCGYC	1 cut(s)	113
Hsp92II	CATG	2 cut(s)	809, 1205
HspAI	GCGC	1 cut(s)	113
KasI	GGCGCC	1 cut(s)	112
Ksp22I	TGATCA	2 cut(s)	51, 340
KspI	CCGCGG	1 cut(s)	1079
LmnI	GCTCC	1 cut(s)	254
Lsp1109I	GCAGC	1 cut(s)	1032
LweI	GCATC	1 cut(s)	937
MaeI	CTAG	3 cut(s)	386, 462, 1148
MaeIII	GTNAC	4 cut(s)	286, 583, 970, 1143
MflI	RGATCY	4 cut(s)	512, 695, 859, 1205
MhlI	GDGCHC	1 cut(s)	404
MluCI	AATT	2 cut(s)	440, 873
MluI	ACGCGT	1 cut(s)	25
Mly113I	GGCGCC	1 cut(s)	113
MlyI	GAGTC	1 cut(s)	455
Mph1103I	ATGCAT	1 cut(s)	1093
MseI	TTAA	4 cut(s)	357, 626, 768, 1223
MslI	CAYNNNNRTG	1 cut(s)	885
MspA1I	CMGCKG	1 cut(s)	1078
MspI	CCGG	4 cut(s)	174, 779, 1168, 1193
MspR9I	CCNGG	3 cut(s)	779, 780, 1035
MvaI	CCWGG	1 cut(s)	1035
MvnI	CGCG	3 cut(s)	27, 840, 1078
MwoI	GCNNNNNNNGC	2 cut(s)	88, 780
NarI	GGCGCC	1 cut(s)	113
NciI	CCSGG	2 cut(s)	779, 780
NlaIII	CATG	2 cut(s)	809, 1205
NlaIV	GGNNCC	2 cut(s)	114, 1052
NmuCI	GTSAC	2 cut(s)	286, 583
NsiI	ATGCAT	1 cut(s)	1093
PfeI	GAWTC	5 cut(s)	165, 748, 995, 1154, 1164
PflMI	CCANNNNNTGG	1 cut(s)	109
PfoI	TCCNGGA	1 cut(s)	1033
PkrI	GCNGC	2 cut(s)	1047, 1050
PleI	GAGTC	1 cut(s)	454
PluTI	GGCGCC	1 cut(s)	116
PpsI	GAGTC	1 cut(s)	454
PshAI	GACNNNNGTC	1 cut(s)	26
Psp124BI	GAGCTC	1 cut(s)	404
Psp6I	CCWGG	1 cut(s)	1033
PspFI	CCCAGC	1 cut(s)	545
PspGI	CCWGG	1 cut(s)	1033
PspN4I	GGNNCC	2 cut(s)	114, 1052
PspPI	GGNCC	1 cut(s)	772
PsuI	RGATCY	4 cut(s)	512, 695, 859, 1205
RsaI	GTAC	5 cut(s)	372, 417, 431, 991, 1027
RsaNI	GTAC	5 cut(s)	371, 416, 430, 990, 1026
RseI	CAYNNNNRTG	1 cut(s)	885
SacI	GAGCTC	1 cut(s)	404
SacII	CCGCGG	1 cut(s)	1079
SalI	GTCGAC	1 cut(s)	1004
SaqAI	TTAA	4 cut(s)	357, 626, 768, 1223
SatI	GCNGC	2 cut(s)	1046, 1049
Sau96I	GGNCC	1 cut(s)	772
SchI	GAGTC	1 cut(s)	455
ScrFI	CCNGG	3 cut(s)	779, 780, 1035
SduI	GDGCHC	1 cut(s)	404
SfaNI	GCATC	1 cut(s)	937
SfcI	CTRYAG	1 cut(s)	261
SfoI	GGCGCC	1 cut(s)	114
Sfr303I	CCGCGG	1 cut(s)	1079
SgrBI	CCGCGG	1 cut(s)	1079
SmaI	CCCGGG	1 cut(s)	780
SmiMI	CAYNNNNRTG	1 cut(s)	885
SmlI	CTYRAG	1 cut(s)	397
SmoI	CTYRAG	1 cut(s)	397
Sse9I	AATT	2 cut(s)	440, 873
SsiI	CCGC	6 cut(s)	840, 1048, 1054, 1076, 1078, 1212
SspDI	GGCGCC	1 cut(s)	112
SspMI	CTAG	3 cut(s)	386, 462, 1148
SstI	GAGCTC	1 cut(s)	404
StyD4I	CCNGG	3 cut(s)	777, 778, 1033
TaaI	ACNGT	5 cut(s)	67, 150, 215, 724, 1221
TaqI	TCGA	2 cut(s)	195, 1005
TaqII	GACCGA	1 cut(s)	705
TasI	AATT	2 cut(s)	440, 873
TatI	WGTACW	2 cut(s)	370, 1025
TauI	GCSGC	1 cut(s)	1051
TfiI	GAWTC	5 cut(s)	165, 748, 995, 1154, 1164
Tru1I	TTAA	4 cut(s)	357, 626, 768, 1223
Tru9I	TTAA	4 cut(s)	357, 626, 768, 1223
TscAI	CASTG	3 cut(s)	343, 386, 484
TseFI	GTSAC	2 cut(s)	286, 583
TseI	GCWGC	1 cut(s)	1045
Tsp45I	GTSAC	2 cut(s)	286, 583
TspDTI	ATGAA	4 cut(s)	150, 320, 498, 956
TspGWI	ACGGA	2 cut(s)	177, 216
TspMI	CCCGGG	1 cut(s)	778
TspRI	CASTG	3 cut(s)	343, 386, 484
Van91I	CCANNNNNTGG	1 cut(s)	109
XagI	CCTNNNNNAGG	1 cut(s)	953
XmaI	CCCGGG	1 cut(s)	778
XmiI	GTMKAC	2 cut(s)	1005, 1071
XspI	CTAG	3 cut(s)	386, 462, 1148
Zsp2I	ATGCAT	1 cut(s)	1093

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.