RchiOBHm_Chr4g0444541

DNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004762
Physical Location & Seq
Reverse (-)
65230772 .. 65236288
5517 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41218

Sequence Viewer

Length: 1500 bp
ATGGCGCCAAGGGCAACCAGAAACCAAAGTCCACCGTGCGATCCTGACGGAATGTTCGCCGGCATGGTCGTCTTTTTAGTCCACAATGGAGTCCAGAACCGTCGATTGCAGATTTGGAAGCAGAAACTGGTCCAAATGGGAGCCACTATAGAATTGCGAGTAACCAAAAATGTCACACACATATTGGCTATGAACCCAGAAGCCCTCTTGCAAGAAGTGGGCAGTGAGCGATTGGGTTTGTTTAAAGGAAGAGTTGTACTATATCAATGGCTACAGGATAGCTTGAGCTCAGGTGAAAAGGTACCTGAAGAATTGTACCTTGTGAATTTGGATGCTGACAAGTCTTCCAGTGATTACAATTCCACTGAAAATGAAACCCCAAATCCCAAAAAGACTAAACTCTCTCCTGAGAATTCACCTCACACTCCAAGCCCTAAAACCCCCAAAGCTCTAAATGAGGATCAGGTCACACCATTGCAGTCATCCTCACCCTACAGCCCACCTGATTTGAACAGCAACATAACTCAGATATTTGGAAAGCTTATCAATATTTATAGAGCCTTGGGCGATGACCGGAGATCTTTTAGCTATTACAAGGCTATACCTGTTATAGAGAGGTTGCCGTTCAAGATACAGAGTCAGGACCAAGTTAAAGGCTTACCTAACATTGGAAAGTCAATGCAAGACCATATTCAGGAAATAGTGACTACCGGGAAGTTATCCAAGTTGGAGCATTTTGAAACAGATGAAAAGGTACGAACTATCAGCTTATTTGGGGAAGTTTGGGGCGTTGGTCCAGCTACTGCACAAAAATTGTATGAGAAAGGACACCGCACATTAGATGATCTGAAGAATGAAGAATCATTAACAAATTCACAGAAGTTAGGGTTAAAATATTTTGATGATATCAAACAGAGAATTCCACGCAATGAGGCTCAGGAGATGGAACGTCTACTACAGAAAGCTGGAGAAGATGTTTTGCCTGGGGTGGACATTGTATGTGGAGGATCATATAGACGCGGGAAAGCTTCTTGTGGGGATCTGGACATTATAATTACACATCCTGATGGAAAAAGTCATAGAGGCTTTTTACCAAAATACGTAAAGCATTTAAAGGATATGAAGTTTCTAAGGGAGGATCTGGTTTTCAGTACACACAGTGAGGAGGGTACTGATTCTGGTGTCGACACATATTTTGGGCTTTGCACATATCCTGGACGAGAGCTGCGCCACCGCATAGATATCAAGGTCTACCCACGGGAAATATATGCATTTGCGCTAATACATTGGACAGGCAATGATGTATTGAATAGGAGGTTGAGAGTTCTAGCCGAATCTAAAGGATTCCTACTTGATGATGATGGGCTATTTCCAGCTACACGGGGCTCTCTCGGTCAACGGGGCATGAGAGGTAGCGCAAGCTTGAAATTTGACACAGAAAAGGAGGTCTTTGAGTTTCTCGGGTTTCCTTGGCTTGAACCACAAGAGAGAAATCTGTGA

Protein Analysis

499

Amino Acids

56.46

Weight (kDa)

7.2

Isoelectric Point (pI)

46.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HHH_8 PF14716 171 - 240 6.1e-14 Helix-hairpin-helix domain
DNA_pol_lambd_f PF10391 257 - 303 4.1e-20 Fingers domain of DNA polymerase lambda
DNA_pol_B_palm PF14792 306 - 420 4.6e-30 DNA polymerase beta palm
DNA_pol_B_thumb PF14791 426 - 498 6.2e-19 DNA polymerase beta thumb
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1052
Acc65I GGTACC 1 cut(s) 301
AccB1I GGYRCC 2 cut(s) 4, 301
AccI GTMKAC 3 cut(s) 952, 1185, 1251
AccII CGCG 1 cut(s) 1020
AciI CCGC 3 cut(s) 832, 1020, 1234
AclWI GGATC 5 cut(s) 35, 468, 1015, 1047, 1146
AcsI RAATTY 5 cut(s) 325, 412, 871, 918, 1427
AcuI CTGAAG 2 cut(s) 327, 869
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 1160
AfaI GTAC 6 cut(s) 258, 303, 317, 756, 1153, 1171
AfiI CCNNNNNNNGG 2 cut(s) 668, 694
AgsI TTSAA 6 cut(s) 511, 628, 740, 1309, 1426, 1478
AjnI CCWGG 2 cut(s) 982, 1213
Alw21I GWGCWC 1 cut(s) 290
AlwI GGATC 5 cut(s) 35, 468, 1015, 1047, 1146
AlwNI CAGNNNCTG 2 cut(s) 127, 803
Ama87I CYCGRG 1 cut(s) 1460
ApeKI GCWGC 1 cut(s) 1225
ApoI RAATTY 5 cut(s) 325, 412, 871, 918, 1427
ArsI GACNNNNNNTTYG 4 cut(s) 38, 70, 1178, 1210
Asp718I GGTACC 1 cut(s) 301
AspLEI GCGC 4 cut(s) 7, 1230, 1279, 1418
AspS9I GGNCC 3 cut(s) 130, 643, 794
AsuC2I CCSGG 1 cut(s) 712
AsuHPI GGTGA 3 cut(s) 305, 408, 480
AvaI CYCGRG 1 cut(s) 1460
AvaII GGWCC 3 cut(s) 130, 643, 794
BanI GGYRCC 2 cut(s) 4, 301
BanII GRGCYC 2 cut(s) 290, 1388
BbsI GAAGAC 1 cut(s) 336
Bbv12I GWGCWC 1 cut(s) 290
BbvI GCAGC 1 cut(s) 1212
BccI CCATC 3 cut(s) 937, 1061, 1355
BceAI ACGGC 1 cut(s) 607
BciT130I CCWGG 2 cut(s) 984, 1215
BcnI CCSGG 1 cut(s) 712
BfaI CTAG 1 cut(s) 1328
BfmI CTRYAG 4 cut(s) 147, 272, 493, 956
BfoI RGCGCY 1 cut(s) 8
BglII AGATCT 1 cut(s) 578
BisI GCNGC 1 cut(s) 1226
BlsI GCNGC 1 cut(s) 1227
Bme1390I CCNGG 3 cut(s) 712, 984, 1215
Bme18I GGWCC 3 cut(s) 130, 643, 794
BmeT110I CYCGRG 1 cut(s) 1460
BmgT120I GGNCC 3 cut(s) 130, 643, 794
BmiI GGNNCC 3 cut(s) 6, 142, 303
BmrFI CCNGG 3 cut(s) 712, 984, 1215
BmsI GCATC 1 cut(s) 322
BpiI GAAGAC 1 cut(s) 336
BpmI CTGGAG 1 cut(s) 987
Bpu10I CCTNAGC 2 cut(s) 289, 936
BpuEI CTTGAG 1 cut(s) 304
BpuMI CCSGG 1 cut(s) 712
BsaAI YACGTR 1 cut(s) 1102
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 5 cut(s) 8, 561, 983, 1256, 1469
BsaWI WCCGGW 1 cut(s) 573
BsaXI ACNNNNNCTCC 2 cut(s) 960, 990
Bsc4I CCNNNNNNNGG 2 cut(s) 668, 694
Bse118I RCCGGY 1 cut(s) 59
Bse1I ACTGG 2 cut(s) 132, 348
Bse3DI GCAATG 3 cut(s) 473, 934, 1303
BseBI CCWGG 2 cut(s) 984, 1215
BseDI CCNNGG 5 cut(s) 8, 561, 983, 1256, 1469
BseGI GGATG 3 cut(s) 337, 482, 1060
BseLI CCNNNNNNNGG 2 cut(s) 668, 694
BseMI GCAATG 3 cut(s) 473, 934, 1303
BseMII CTCAG 4 cut(s) 303, 399, 539, 950
BseNI ACTGG 2 cut(s) 132, 348
BseRI GAGGAG 1 cut(s) 1178
BseXI GCAGC 1 cut(s) 1212
BsgI GTGCAG 1 cut(s) 789
Bsh1236I CGCG 1 cut(s) 1020
BshNI GGYRCC 2 cut(s) 4, 301
BsiHKAI GWGCWC 1 cut(s) 290
BsiHKCI CYCGRG 1 cut(s) 1460
BsiSI CCGG 3 cut(s) 60, 574, 711
BslI CCNNNNNNNGG 2 cut(s) 668, 694
BsoBI CYCGRG 1 cut(s) 1460
Bsp1286I GDGCHC 2 cut(s) 290, 1388
Bsp143I GATC 7 cut(s) 40, 460, 578, 844, 1007, 1039, 1138
BspACI CCGC 3 cut(s) 832, 1020, 1234
BspCNI CTCAG 4 cut(s) 302, 400, 538, 949
BspFNI CGCG 1 cut(s) 1020
BspLI GGNNCC 3 cut(s) 6, 142, 303
BspPI GGATC 5 cut(s) 35, 468, 1015, 1047, 1146
BspT107I GGYRCC 2 cut(s) 4, 301
BsrDI GCAATG 3 cut(s) 473, 934, 1303
BsrFI RCCGGY 1 cut(s) 59
BsrI ACTGG 2 cut(s) 132, 348
BssAI RCCGGY 1 cut(s) 59
BssECI CCNNGG 5 cut(s) 8, 561, 983, 1256, 1469
BssMI GATC 7 cut(s) 40, 460, 578, 844, 1007, 1039, 1138
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 3 cut(s) 8, 561, 1469
Bst2UI CCWGG 2 cut(s) 984, 1215
Bst4CI ACNGT 3 cut(s) 36, 101, 1160
Bst6I CTCTTC 1 cut(s) 244
BstACI GRCGYC 1 cut(s) 5
BstBAI YACGTR 1 cut(s) 1102
BstC8I GCNNGC 2 cut(s) 61, 1420
BstDEI CTNAG 5 cut(s) 289, 408, 525, 936, 1130
BstDSI CCRYGG 1 cut(s) 1256
BstF5I GGATG 3 cut(s) 337, 482, 1060
BstFNI CGCG 1 cut(s) 1020
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 4 cut(s) 7, 1230, 1279, 1418
BstKTI GATC 7 cut(s) 43, 463, 581, 847, 1010, 1042, 1141
BstMBI GATC 7 cut(s) 40, 460, 578, 844, 1007, 1039, 1138
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 2 cut(s) 984, 1215
BstSCI CCNGG 3 cut(s) 710, 982, 1213
BstSFI CTRYAG 4 cut(s) 147, 272, 493, 956
BstSNI TACGTA 1 cut(s) 1102
BstUI CGCG 1 cut(s) 1020
BstV1I GCAGC 1 cut(s) 1212
BstV2I GAAGAC 1 cut(s) 336
BstX2I RGATCY 3 cut(s) 578, 1039, 1138
BstYI RGATCY 3 cut(s) 578, 1039, 1138
BtgI CCRYGG 1 cut(s) 1256
BtgZI GCGATG 1 cut(s) 582
BtsCI GGATG 3 cut(s) 337, 482, 1060
BtsI GCAGTG 1 cut(s) 229
BtsIMutI CAGTG 4 cut(s) 229, 355, 363, 1165
Cac8I GCNNGC 2 cut(s) 61, 1420
CaiI CAGNNNCTG 2 cut(s) 127, 803
CfoI GCGC 4 cut(s) 7, 1230, 1279, 1418
Cfr10I RCCGGY 1 cut(s) 59
Cfr13I GGNCC 3 cut(s) 130, 643, 794
CseI GACGC 1 cut(s) 1026
Csp6I GTAC 6 cut(s) 257, 302, 316, 755, 1152, 1170
CspCI CAANNNNNGTGG 2 cut(s) 489, 524
CviAII CATG 2 cut(s) 64, 1405
CviQI GTAC 6 cut(s) 257, 302, 316, 755, 1152, 1170
DdeI CTNAG 5 cut(s) 289, 408, 525, 936, 1130
DinI GGCGCC 1 cut(s) 6
DpnI GATC 7 cut(s) 42, 462, 580, 846, 1009, 1041, 1140
DpnII GATC 7 cut(s) 40, 460, 578, 844, 1007, 1039, 1138
DraI TTTAAA 2 cut(s) 244, 1113
DraIII CACNNNGTG 1 cut(s) 1160
Eam1104I CTCTTC 1 cut(s) 244
EarI CTCTTC 1 cut(s) 244
Ecl136II GAGCTC 1 cut(s) 288
Eco105I TACGTA 1 cut(s) 1102
Eco130I CCWWGG 3 cut(s) 8, 561, 1469
Eco24I GRGCYC 2 cut(s) 290, 1388
Eco32I GATATC 2 cut(s) 907, 1243
Eco47I GGWCC 3 cut(s) 130, 643, 794
Eco53kI GAGCTC 1 cut(s) 288
Eco57I CTGAAG 2 cut(s) 327, 869
Eco88I CYCGRG 1 cut(s) 1460
EcoICRI GAGCTC 1 cut(s) 288
EcoRI GAATTC 2 cut(s) 412, 918
EcoRII CCWGG 2 cut(s) 982, 1213
EcoRV GATATC 2 cut(s) 907, 1243
EcoT14I CCWWGG 3 cut(s) 8, 561, 1469
EcoT22I ATGCAT 1 cut(s) 1273
EcoT38I GRGCYC 2 cut(s) 290, 1388
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 3 cut(s) 8, 561, 1469
FaeI CATG 2 cut(s) 67, 1408
FalI AAGNNNNNCTT 2 cut(s) 1433, 1465
FatI CATG 2 cut(s) 63, 1404
FauI CCCGC 1 cut(s) 1013
FblI GTMKAC 3 cut(s) 952, 1185, 1251
Fnu4HI GCNGC 1 cut(s) 1226
FokI GGATG 3 cut(s) 344, 469, 1047
FriOI GRGCYC 2 cut(s) 290, 1388
Fsp4HI GCNGC 1 cut(s) 1226
FspBI CTAG 1 cut(s) 1328
GlaI GCGC 4 cut(s) 6, 1229, 1278, 1417
GluI GCNGC 1 cut(s) 1226
GsuI CTGGAG 1 cut(s) 987
HaeII RGCGCY 1 cut(s) 8
HapII CCGG 3 cut(s) 60, 574, 711
HgaI GACGC 1 cut(s) 1026
HhaI GCGC 4 cut(s) 7, 1230, 1279, 1418
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 67, 1408
Hin6I GCGC 4 cut(s) 5, 1228, 1277, 1416
HinP1I GCGC 4 cut(s) 5, 1228, 1277, 1416
HincII GTYRAC 2 cut(s) 1186, 1397
HindII GTYRAC 2 cut(s) 1186, 1397
HindIII AAGCTT 3 cut(s) 539, 1026, 1420
HinfI GANTC 6 cut(s) 90, 637, 860, 1175, 1334, 1344
HpaII CCGG 3 cut(s) 60, 574, 711
HphI GGTGA 3 cut(s) 305, 408, 480
Hpy166II GTNNAC 8 cut(s) 32, 82, 953, 991, 1154, 1186, 1252, 1397
Hpy188I TCNGA 2 cut(s) 528, 849
Hpy188III TCNNGA 9 cut(s) 44, 94, 407, 628, 641, 695, 938, 1043, 1064
Hpy8I GTNNAC 8 cut(s) 32, 82, 953, 991, 1154, 1186, 1252, 1397
Hpy99I CGWCG 1 cut(s) 105
HpyCH4III ACNGT 3 cut(s) 36, 101, 1160
HpyCH4IV ACGT 2 cut(s) 949, 1101
HpyCH4V TGCA 7 cut(s) 109, 211, 478, 682, 806, 1206, 1271
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 5 cut(s) 289, 408, 525, 936, 1130
HpySE526I ACGT 2 cut(s) 949, 1101
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 67, 1408
HspAI GCGC 4 cut(s) 5, 1228, 1277, 1416
KasI GGCGCC 1 cut(s) 4
KpnI GGTACC 1 cut(s) 305
KroI GCCGGC 1 cut(s) 59
KroNI GCCGGC 1 cut(s) 61
Kzo9I GATC 7 cut(s) 40, 460, 578, 844, 1007, 1039, 1138
LmnI GCTCC 2 cut(s) 140, 730
Lsp1109I GCAGC 1 cut(s) 1212
LweI GCATC 1 cut(s) 322
MaeI CTAG 1 cut(s) 1328
MaeII ACGT 2 cut(s) 949, 1101
MaeIII GTNAC 4 cut(s) 160, 172, 466, 703
MalI GATC 7 cut(s) 42, 462, 580, 846, 1009, 1041, 1140
MboI GATC 7 cut(s) 40, 460, 578, 844, 1007, 1039, 1138
MboII GAAGA 6 cut(s) 261, 320, 336, 862, 869, 983
MflI RGATCY 3 cut(s) 578, 1039, 1138
MhlI GDGCHC 2 cut(s) 290, 1388
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 2 cut(s) 99, 646
MmeI TCCRAC 1 cut(s) 708
Mph1103I ATGCAT 1 cut(s) 1273
MroNI GCCGGC 1 cut(s) 59
MseI TTAA 5 cut(s) 243, 651, 866, 890, 1112
MslI CAYNNNNRTG 1 cut(s) 1065
MspI CCGG 3 cut(s) 60, 574, 711
MspR9I CCNGG 3 cut(s) 712, 984, 1215
MvaI CCWGG 2 cut(s) 984, 1215
MvnI CGCG 1 cut(s) 1020
MwoI GCNNNNNNNGC 1 cut(s) 11
NaeI GCCGGC 1 cut(s) 61
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 712
NdeII GATC 7 cut(s) 40, 460, 578, 844, 1007, 1039, 1138
NgoMIV GCCGGC 1 cut(s) 59
NlaIII CATG 2 cut(s) 67, 1408
NlaIV GGNNCC 3 cut(s) 6, 142, 303
NmuCI GTSAC 3 cut(s) 172, 466, 703
NsiI ATGCAT 1 cut(s) 1273
PdiI GCCGGC 1 cut(s) 61
PfeI GAWTC 4 cut(s) 860, 1175, 1334, 1344
PfoI TCCNGGA 1 cut(s) 1213
PkrI GCNGC 1 cut(s) 1227
PleI GAGTC 2 cut(s) 98, 645
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 2 cut(s) 98, 645
Ppu21I YACGTR 1 cut(s) 1102
PsiI TTATAA 1 cut(s) 1052
Psp124BI GAGCTC 1 cut(s) 290
Psp6I CCWGG 2 cut(s) 982, 1213
PspGI CCWGG 2 cut(s) 982, 1213
PspN4I GGNNCC 3 cut(s) 6, 142, 303
PspPI GGNCC 3 cut(s) 130, 643, 794
PsrI GAACNNNNNNTAC 2 cut(s) 939, 971
PstNI CAGNNNCTG 2 cut(s) 127, 803
PsuI RGATCY 3 cut(s) 578, 1039, 1138
RsaI GTAC 6 cut(s) 258, 303, 317, 756, 1153, 1171
RsaNI GTAC 6 cut(s) 257, 302, 316, 755, 1152, 1170
RseI CAYNNNNRTG 1 cut(s) 1065
SacI GAGCTC 1 cut(s) 290
SalI GTCGAC 1 cut(s) 1184
SaqAI TTAA 5 cut(s) 243, 651, 866, 890, 1112
SatI GCNGC 1 cut(s) 1226
Sau3AI GATC 7 cut(s) 40, 460, 578, 844, 1007, 1039, 1138
Sau96I GGNCC 3 cut(s) 130, 643, 794
SchI GAGTC 2 cut(s) 99, 646
ScrFI CCNGG 3 cut(s) 712, 984, 1215
SduI GDGCHC 2 cut(s) 290, 1388
SfaNI GCATC 1 cut(s) 322
SfcI CTRYAG 4 cut(s) 147, 272, 493, 956
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 3 cut(s) 130, 643, 794
SmiMI CAYNNNNRTG 1 cut(s) 1065
SmlI CTYRAG 1 cut(s) 283
SmoI CTYRAG 1 cut(s) 283
SnaBI TACGTA 1 cut(s) 1102
SsiI CCGC 3 cut(s) 832, 1020, 1234
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 2 cut(s) 550, 896
SspMI CTAG 1 cut(s) 1328
SstI GAGCTC 1 cut(s) 290
StyD4I CCNGG 3 cut(s) 710, 982, 1213
StyI CCWWGG 3 cut(s) 8, 561, 1469
TaaI ACNGT 3 cut(s) 36, 101, 1160
TaiI ACGT 2 cut(s) 952, 1104
TaqI TCGA 2 cut(s) 103, 1185
TaqII GACCGA 1 cut(s) 1382
TatI WGTACW 2 cut(s) 256, 1151
TfiI GAWTC 4 cut(s) 860, 1175, 1334, 1344
Tru1I TTAA 5 cut(s) 243, 651, 866, 890, 1112
Tru9I TTAA 5 cut(s) 243, 651, 866, 890, 1112
TscAI CASTG 4 cut(s) 229, 355, 370, 1165
TseFI GTSAC 3 cut(s) 172, 466, 703
TseI GCWGC 1 cut(s) 1225
Tsp45I GTSAC 3 cut(s) 172, 466, 703
TspDTI ATGAA 5 cut(s) 206, 387, 762, 870, 1136
TspGWI ACGGA 1 cut(s) 63
TspRI CASTG 4 cut(s) 229, 355, 370, 1165
VpaK11BI GGWCC 3 cut(s) 130, 643, 794
XapI RAATTY 5 cut(s) 325, 412, 871, 918, 1427
XmiI GTMKAC 3 cut(s) 952, 1185, 1251
XspI CTAG 1 cut(s) 1328
Zsp2I ATGCAT 1 cut(s) 1273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.