Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0004762

Physical Location & Seq

Genomic Coordinates Reverse (-)

62350432 .. 62354335

Gene Structure 3904 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G035550.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1509 bp

ATGGCGCCAAAAAAGGCAACCAGAGATGAAAGTCCTCCACCGTCCGATCCTCACGGAATCTTCGCCGGAATGGTCGTCTTCTTAGTCGAAGACGGAGTTCAGAACCGTCGCTTACAGGTTTGGAAGCAAAAACTGGTTCAAATGGGAGCTACTATAGAAGATCATCTATCCAAAAGGGTCACACACATATTTGCTATGAACCCAGAAGCCCTTTTGCAAAAAGTGGGCAGTGATCAATGGGGTCTCTTTAAAGGAAGAGTTGTACTCTATCAGTGGCTAGAGGACAGCTTAAGCTCAGGGAAGAGAGTACCAGAAGATTTGTACCATCTGAAATTGGAGTCAGATAAGTCTTCTAGTGATTACATTTCCAGTGATGAAGAAACCCCAAAACTGAAAAGGCGAAGATCCTCATCTCCTGATGAGTTGCCTCATACTCCGAGCCCCAAAACCCCTAAAGCCCCAAATAAGGATCAGGTCACAGCATTGCAGTCATCCACGCCCTACAACCCACCTGATTTAAACAAAGACATAACCCAGATATTTGGGAAGCTTATCAACATATATAGAGCATTGGGCGATGACCGAAGATCTTTTAGCTACTACAAGGCTATAACAGTTATTGAGAAGTTGCCTTTCAAGATTCAAAGTGCAGAACAAGTTAAGGGCCTCCCGGGCATTGGAAAGTCAATGCAAGATCATATTCAAGAGATAGTGACTACCGGAAAGCTATCCAAGTTGGAGCACTTTGAAACAGATGAAAAGGTAAAAACAATCAGCTTATTCGAGGAAATATGGGGTGTAGGTCCAGCTACTGCACTAAAATTATTTGAGAAAGGACATCACACATTAGATGATTTGAAGAATGAAGATTCGTTGACACATTCACAGAAGTTGGGGTTAAGATATTATGAGGATATCAAACAGAGGATCCCACGGCATGAGGCTCAAGAGATGGAACTTCTTCTACAAAAAGCGGCAGAGGATGTTTTGCCTGGGGTGGTTGTTGTATGTGGGGGGTCATATAGACGCTGGAAATCTTCTTGTGGGGATCTGGACATTGTAATTACTCATCCAGATGGGAACAGTCATAAAGGTTTTTTACCGAAGTATGTAAAGCATCTAAAGGATATGAAGTTCCTAAGGGAGGATTTGGTTTTCGTTACAAACAGCAAGGAGGGTACTGATTCTGGTGTCGACACATATTATGGGCTTTGTACATATCCTGGACGAGAGCTGCGGCACCGCATAGATTTCAAGGTCTACCCGCGGGACATATATGCATTTGGACTAATAGCTTGGACAGGGAATGATGTGCTGAACAGGAGGTTGAGGTTACTAGCTGAATCTAAAGGATTCCGGCTTGATGATACGGGGCTGTTTCCGGCCACTCATGGATCTGGCGGAAAACGGGGGGCAAAAGCTGGCGCAAGCTTGAAATTTGACACAGAAAAGGAGGTCTTTGACTTTCTCGGGTTTCCTTGGCTTGAACCACAAGAGAGAAATCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0000726 GO:0003674 GO:0003824 GO:0003887 GO:0005488 GO:0006139 GO:0006259 GO:0006281 GO:0006284 GO:0006285 GO:0006287 GO:0006289 GO:0006302 GO:0006303 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0010224 GO:0016740 GO:0016772 GO:0016779 GO:0016829 GO:0016835 GO:0018130 GO:0019438 GO:0030145 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0043167 GO:0043169 GO:0043170 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0046483 GO:0046872 GO:0046914 GO:0050896 GO:0051575 GO:0051716 GO:0071704 GO:0071897 GO:0090304 GO:0097510 GO:0140097 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03410 ko03450 map03410 map03450

Pfam Domains

Protein Families

BRCT DNA_pol_B_palm DNA_pol_B_thumb DNA_pol_lambd_f HHH_8 LIM

Protein Analysis

502

Amino Acids

56.87

Weight (kDa)

8.4

Isoelectric Point (pI)

48.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HHH_8	PF14716	174 - 243	5.5e-16	Helix-hairpin-helix domain
DNA_pol_lambd_f	PF10391	260 - 306	8.1e-19	Fingers domain of DNA polymerase lambda
DNA_pol_B_palm	PF14792	309 - 423	2.7e-28	DNA polymerase beta palm
DNA_pol_B_thumb	PF14791	430 - 501	1.6e-19	DNA polymerase beta thumb

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004762)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10520 AT1G10520
fragaria_vesca	FvH4_4g35010 FvH4_4g35030
malus_domestica	MD16G1086600.v1.1 MD16G1086700.v1.1
prunus_persica	Prupe.1G259900_v2.0.a1 Prupe.1G259900_v2.0.a1 Prupe.1G259900_v2.0.a1 Prupe.1G259900_v2.0.a1
pyrus_communis	pycom16g07470
rosa_chinensis	RchiOBHm_Chr4g0444541 RchiOBHm_Chr4g0444551
rosa_laevigata	RLG00000005822 RLG00000005825
rosa_multiflora	Rmu_sc0003839.1_g000005 Rmu_sc0012648.1_g000007
rosa_roxburghii	Rroxscaffold_5G00385000 Rroxscaffold_5G00385010
rosa_rugosa	Rorug04G0353500 Rorug04G0353600 Rorug04G0353700 Rorug04G0353800
rosa_samantha	Rh4AG413800 Rh4BG425500 Rh4CG439900 Rh4CG440200 Rh4DG420800 Rh4DG421100
rosa_wichuraiana	Rw4G035540 Rw4G035550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	4, 1239
AccI	GTMKAC	2 cut(s)	1194, 1260
AccII	CGCG	1 cut(s)	1267
AciI	CCGC	6 cut(s)	974, 1237, 1243, 1265, 1267, 1401
AclWI	GGATC	7 cut(s)	41, 399, 477, 922, 935, 1056, 1402
AcoI	YGGCCR	1 cut(s)	1383
AcsI	RAATTY	1 cut(s)	1436
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	5 cut(s)	264, 309, 323, 1180, 1216
AfiI	CCNNNNNNNGG	3 cut(s)	466, 677, 1144
AflII	CTTAAG	1 cut(s)	289
AgsI	TTSAA	9 cut(s)	140, 637, 644, 704, 749, 859, 1255, 1435, 1487
AjnI	CCWGG	2 cut(s)	991, 1222
AloI	GAACNNNNNNTCC	2 cut(s)	1118, 1150
Alw21I	GWGCWC	1 cut(s)	744
Alw26I	GTCTC	1 cut(s)	248
AlwI	GGATC	7 cut(s)	41, 399, 477, 922, 935, 1056, 1402
AlwNI	CAGNNNCTG	1 cut(s)	812
Ama87I	CYCGRG	2 cut(s)	670, 1469
AoxI	GGCC	2 cut(s)	664, 1383
ApeKI	GCWGC	1 cut(s)	1234
ApoI	RAATTY	1 cut(s)	1436
Asp700I	GAANNNNTTC	1 cut(s)	960
AspLEI	GCGC	2 cut(s)	7, 1427
AspS9I	GGNCC	2 cut(s)	664, 803
AsuC2I	CCSGG	2 cut(s)	671, 672
AvaI	CYCGRG	2 cut(s)	670, 1469
AvaII	GGWCC	1 cut(s)	803
AxyI	CCTNAGG	1 cut(s)	1139
BamHI	GGATCC	1 cut(s)	927
BanI	GGYRCC	2 cut(s)	4, 1239
BanII	GRGCYC	1 cut(s)	443
BbsI	GAAGAC	3 cut(s)	70, 96, 342
Bbv12I	GWGCWC	1 cut(s)	744
BbvI	GCAGC	1 cut(s)	1221
BccI	CCATC	3 cut(s)	333, 946, 1070
BceAI	ACGGC	1 cut(s)	950
BciT130I	CCWGG	2 cut(s)	993, 1224
BclI	TGATCA	1 cut(s)	232
BcnI	CCSGG	2 cut(s)	671, 672
BcoDI	GTCTC	1 cut(s)	248
BfaI	CTAG	3 cut(s)	278, 354, 1337
BfmI	CTRYAG	1 cut(s)	153
BfoI	RGCGCY	1 cut(s)	8
BfrI	CTTAAG	1 cut(s)	289
BglI	GCCNNNNNGGC	1 cut(s)	672
BglII	AGATCT	1 cut(s)	587
BisI	GCNGC	3 cut(s)	975, 1235, 1238
BlsI	GCNGC	3 cut(s)	976, 1236, 1239
Bme1390I	CCNGG	4 cut(s)	671, 672, 993, 1224
Bme18I	GGWCC	1 cut(s)	803
BmeT110I	CYCGRG	2 cut(s)	670, 1469
BmgT120I	GGNCC	2 cut(s)	664, 803
BmiI	GGNNCC	3 cut(s)	6, 929, 1241
BmrFI	CCNGG	4 cut(s)	671, 672, 993, 1224
BmsI	GCATC	1 cut(s)	1126
BpiI	GAAGAC	3 cut(s)	70, 96, 342
BplI	GAGNNNNNCTC	2 cut(s)	249, 281
Bpu10I	CCTNAGC	1 cut(s)	295
BpuEI	CTTGAG	1 cut(s)	930
BpuMI	CCSGG	2 cut(s)	671, 672
BsaBI	GATNNNNATC	1 cut(s)	409
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	1 cut(s)	248
BsaJI	CCNNGG	5 cut(s)	670, 932, 992, 1265, 1478
BsaWI	WCCGGW	1 cut(s)	719
Bsc4I	CCNNNNNNNGG	3 cut(s)	466, 677, 1144
Bse1I	ACTGG	2 cut(s)	138, 369
Bse21I	CCTNAGG	1 cut(s)	1139
Bse3DI	GCAATG	1 cut(s)	482
Bse8I	GATNNNNATC	1 cut(s)	409
BseBI	CCWGG	2 cut(s)	993, 1224
BseDI	CCNNGG	5 cut(s)	670, 932, 992, 1265, 1478
BseGI	GGATG	3 cut(s)	491, 988, 1069
BseJI	GATNNNNATC	1 cut(s)	409
BseLI	CCNNNNNNNGG	3 cut(s)	466, 677, 1144
BseMI	GCAATG	1 cut(s)	482
BseMII	CTCAG	1 cut(s)	309
BseNI	ACTGG	2 cut(s)	138, 369
BseXI	GCAGC	1 cut(s)	1221
BsgI	GTGCAG	2 cut(s)	669, 798
Bsh1236I	CGCG	1 cut(s)	1267
BshFI	GGCC	2 cut(s)	666, 1385
BshNI	GGYRCC	2 cut(s)	4, 1239
BsiHKAI	GWGCWC	1 cut(s)	744
BsiHKCI	CYCGRG	2 cut(s)	670, 1469
BsiSI	CCGG	5 cut(s)	66, 671, 720, 1357, 1382
BslFI	GGGAC	1 cut(s)	1283
BslI	CCNNNNNNNGG	3 cut(s)	466, 677, 1144
BsmAI	GTCTC	1 cut(s)	248
BsmFI	GGGAC	1 cut(s)	1283
BsnI	GGCC	2 cut(s)	666, 1385
Bso31I	GGTCTC	1 cut(s)	248
BsoBI	CYCGRG	2 cut(s)	670, 1469
Bsp1286I	GDGCHC	2 cut(s)	443, 744
Bsp1407I	TGTACA	1 cut(s)	1214
BspACI	CCGC	6 cut(s)	974, 1237, 1243, 1265, 1267, 1401
BspANI	GGCC	2 cut(s)	666, 1385
BspCNI	CTCAG	1 cut(s)	308
BspFNI	CGCG	1 cut(s)	1267
BspLI	GGNNCC	3 cut(s)	6, 929, 1241
BspPI	GGATC	7 cut(s)	41, 399, 477, 922, 935, 1056, 1402
BspT107I	GGYRCC	2 cut(s)	4, 1239
BspTI	CTTAAG	1 cut(s)	289
BspTNI	GGTCTC	1 cut(s)	248
BsrDI	GCAATG	1 cut(s)	482
BsrGI	TGTACA	1 cut(s)	1214
BsrI	ACTGG	2 cut(s)	138, 369
BssECI	CCNNGG	5 cut(s)	670, 932, 992, 1265, 1478
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	1 cut(s)	1478
Bst2UI	CCWGG	2 cut(s)	993, 1224
Bst4CI	ACNGT	4 cut(s)	42, 107, 616, 1085
Bst6I	CTCTTC	2 cut(s)	250, 296
BstACI	GRCGYC	1 cut(s)	5
BstAFI	CTTAAG	1 cut(s)	289
BstAUI	TGTACA	1 cut(s)	1214
BstC8I	GCNNGC	2 cut(s)	1423, 1429
BstDEI	CTNAG	3 cut(s)	82, 295, 1139
BstDSI	CCRYGG	2 cut(s)	932, 1265
BstENI	CCTNNNNNAGG	1 cut(s)	1142
BstF5I	GGATG	3 cut(s)	491, 988, 1069
BstFNI	CGCG	1 cut(s)	1267
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	2 cut(s)	7, 1427
BstMAI	GTCTC	1 cut(s)	248
BstMWI	GCNNNNNNNGC	1 cut(s)	672
BstNI	CCWGG	2 cut(s)	993, 1224
BstSCI	CCNGG	4 cut(s)	669, 670, 991, 1222
BstSFI	CTRYAG	1 cut(s)	153
BstUI	CGCG	1 cut(s)	1267
BstV1I	GCAGC	1 cut(s)	1221
BstV2I	GAAGAC	3 cut(s)	70, 96, 342
BstX2I	RGATCY	5 cut(s)	404, 587, 927, 1048, 1394
BstXI	CCANNNNNNTGG	1 cut(s)	542
BstYI	RGATCY	5 cut(s)	404, 587, 927, 1048, 1394
Bsu36I	CCTNAGG	1 cut(s)	1139
BsuRI	GGCC	2 cut(s)	666, 1385
BtgI	CCRYGG	2 cut(s)	932, 1265
BtgZI	GCGATG	1 cut(s)	591
BtsCI	GGATG	3 cut(s)	491, 988, 1069
BtsI	GCAGTG	1 cut(s)	235
BtsIMutI	CAGTG	3 cut(s)	235, 278, 376
Cac8I	GCNNGC	2 cut(s)	1423, 1429
CaiI	CAGNNNCTG	1 cut(s)	812
CfoI	GCGC	2 cut(s)	7, 1427
Cfr13I	GGNCC	2 cut(s)	664, 803
Cfr42I	CCGCGG	1 cut(s)	1268
Cfr9I	CCCGGG	1 cut(s)	670
CseI	GACGC	1 cut(s)	1035
Csp6I	GTAC	5 cut(s)	263, 308, 322, 1179, 1215
CviAII	CATG	2 cut(s)	938, 1391
CviQI	GTAC	5 cut(s)	263, 308, 322, 1179, 1215
DdeI	CTNAG	3 cut(s)	82, 295, 1139
DinI	GGCGCC	1 cut(s)	6
DraI	TTTAAA	2 cut(s)	250, 519
EaeI	YGGCCR	1 cut(s)	1383
Eam1104I	CTCTTC	2 cut(s)	250, 296
EarI	CTCTTC	2 cut(s)	250, 296
EciI	GGCGGA	1 cut(s)	1416
Eco130I	CCWWGG	1 cut(s)	1478
Eco24I	GRGCYC	1 cut(s)	443
Eco31I	GGTCTC	1 cut(s)	248
Eco32I	GATATC	1 cut(s)	916
Eco47I	GGWCC	1 cut(s)	803
Eco81I	CCTNAGG	1 cut(s)	1139
Eco88I	CYCGRG	2 cut(s)	670, 1469
EcoNI	CCTNNNNNAGG	1 cut(s)	1142
EcoO109I	RGGNCCY	1 cut(s)	664
EcoRII	CCWGG	2 cut(s)	991, 1222
EcoRV	GATATC	1 cut(s)	916
EcoT14I	CCWWGG	1 cut(s)	1478
EcoT22I	ATGCAT	1 cut(s)	1282
EcoT38I	GRGCYC	1 cut(s)	443
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
ErhI	CCWWGG	1 cut(s)	1478
FaeI	CATG	2 cut(s)	941, 1394
FalI	AAGNNNNNCTT	2 cut(s)	1442, 1474
FaqI	GGGAC	1 cut(s)	1283
FatI	CATG	2 cut(s)	937, 1390
FauI	CCCGC	2 cut(s)	1260, 1272
FbaI	TGATCA	1 cut(s)	232
FblI	GTMKAC	2 cut(s)	1194, 1260
Fnu4HI	GCNGC	3 cut(s)	975, 1235, 1238
FokI	GGATG	3 cut(s)	478, 995, 1056
FriOI	GRGCYC	1 cut(s)	443
Fsp4HI	GCNGC	3 cut(s)	975, 1235, 1238
FspBI	CTAG	3 cut(s)	278, 354, 1337
GlaI	GCGC	2 cut(s)	6, 1426
GluI	GCNGC	3 cut(s)	975, 1235, 1238
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	2 cut(s)	666, 1385
HapII	CCGG	5 cut(s)	66, 671, 720, 1357, 1382
HgaI	GACGC	1 cut(s)	1035
HhaI	GCGC	2 cut(s)	7, 1427
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	2 cut(s)	941, 1394
Hin6I	GCGC	2 cut(s)	5, 1425
HinP1I	GCGC	2 cut(s)	5, 1425
HincII	GTYRAC	2 cut(s)	876, 1195
HindII	GTYRAC	2 cut(s)	876, 1195
HindIII	AAGCTT	2 cut(s)	548, 1429
HinfI	GANTC	7 cut(s)	57, 338, 640, 869, 1184, 1343, 1353
HpaII	CCGG	5 cut(s)	66, 671, 720, 1357, 1382
Hpy166II	GTNNAC	3 cut(s)	876, 1195, 1261
Hpy188I	TCNGA	5 cut(s)	46, 102, 330, 343, 438
Hpy188III	TCNNGA	6 cut(s)	416, 637, 704, 947, 1052, 1073
Hpy8I	GTNNAC	3 cut(s)	876, 1195, 1261
Hpy99I	CGWCG	1 cut(s)	111
HpyCH4III	ACNGT	4 cut(s)	42, 107, 616, 1085
HpyCH4V	TGCA	6 cut(s)	217, 487, 650, 691, 815, 1280
HpyF10VI	GCNNNNNNNGC	1 cut(s)	672
HpyF3I	CTNAG	3 cut(s)	82, 295, 1139
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	2 cut(s)	941, 1394
HspAI	GCGC	2 cut(s)	5, 1425
KasI	GGCGCC	1 cut(s)	4
Ksp22I	TGATCA	1 cut(s)	232
KspI	CCGCGG	1 cut(s)	1268
LmnI	GCTCC	2 cut(s)	146, 739
Lsp1109I	GCAGC	1 cut(s)	1221
LweI	GCATC	1 cut(s)	1126
MaeI	CTAG	3 cut(s)	278, 354, 1337
MaeIII	GTNAC	5 cut(s)	178, 475, 712, 1159, 1332
MflI	RGATCY	5 cut(s)	404, 587, 927, 1048, 1394
MhlI	GDGCHC	2 cut(s)	443, 744
MluCI	AATT	4 cut(s)	332, 821, 1062, 1436
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	1 cut(s)	347
MmeI	TCCRAC	1 cut(s)	717
Mph1103I	ATGCAT	1 cut(s)	1282
MroXI	GAANNNNTTC	1 cut(s)	960
MseI	TTAA	5 cut(s)	249, 290, 518, 660, 899
MslI	CAYNNNNRTG	1 cut(s)	1074
MspA1I	CMGCKG	1 cut(s)	1267
MspCI	CTTAAG	1 cut(s)	289
MspI	CCGG	5 cut(s)	66, 671, 720, 1357, 1382
MspR9I	CCNGG	4 cut(s)	671, 672, 993, 1224
MvaI	CCWGG	2 cut(s)	993, 1224
MvnI	CGCG	1 cut(s)	1267
MwoI	GCNNNNNNNGC	1 cut(s)	672
NarI	GGCGCC	1 cut(s)	5
NciI	CCSGG	2 cut(s)	671, 672
NlaIII	CATG	2 cut(s)	941, 1394
NlaIV	GGNNCC	3 cut(s)	6, 929, 1241
NmuCI	GTSAC	3 cut(s)	178, 475, 712
NsiI	ATGCAT	1 cut(s)	1282
PdmI	GAANNNNTTC	1 cut(s)	960
PfeI	GAWTC	6 cut(s)	57, 640, 869, 1184, 1343, 1353
PfoI	TCCNGGA	1 cut(s)	1222
PkrI	GCNGC	3 cut(s)	976, 1236, 1239
PleI	GAGTC	1 cut(s)	346
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	1 cut(s)	346
Psp6I	CCWGG	2 cut(s)	991, 1222
PspGI	CCWGG	2 cut(s)	991, 1222
PspN4I	GGNNCC	3 cut(s)	6, 929, 1241
PspPI	GGNCC	2 cut(s)	664, 803
PsrI	GAACNNNNNNTAC	2 cut(s)	948, 980
PstNI	CAGNNNCTG	1 cut(s)	812
PsuI	RGATCY	5 cut(s)	404, 587, 927, 1048, 1394
RsaI	GTAC	5 cut(s)	264, 309, 323, 1180, 1216
RsaNI	GTAC	5 cut(s)	263, 308, 322, 1179, 1215
RseI	CAYNNNNRTG	1 cut(s)	1074
SacII	CCGCGG	1 cut(s)	1268
SalI	GTCGAC	1 cut(s)	1193
SaqAI	TTAA	5 cut(s)	249, 290, 518, 660, 899
SatI	GCNGC	3 cut(s)	975, 1235, 1238
Sau96I	GGNCC	2 cut(s)	664, 803
SchI	GAGTC	1 cut(s)	347
ScrFI	CCNGG	4 cut(s)	671, 672, 993, 1224
SduI	GDGCHC	2 cut(s)	443, 744
SfaNI	GCATC	1 cut(s)	1126
SfcI	CTRYAG	1 cut(s)	153
SfoI	GGCGCC	1 cut(s)	6
Sfr303I	CCGCGG	1 cut(s)	1268
SgrBI	CCGCGG	1 cut(s)	1268
SinI	GGWCC	1 cut(s)	803
SmaI	CCCGGG	1 cut(s)	672
SmiMI	CAYNNNNRTG	1 cut(s)	1074
SmlI	CTYRAG	2 cut(s)	289, 945
SmoI	CTYRAG	2 cut(s)	289, 945
Sse9I	AATT	4 cut(s)	332, 821, 1062, 1436
SsiI	CCGC	6 cut(s)	974, 1237, 1243, 1265, 1267, 1401
SspDI	GGCGCC	1 cut(s)	4
SspMI	CTAG	3 cut(s)	278, 354, 1337
StyD4I	CCNGG	4 cut(s)	669, 670, 991, 1222
StyI	CCWWGG	1 cut(s)	1478
TaaI	ACNGT	4 cut(s)	42, 107, 616, 1085
TaqI	TCGA	3 cut(s)	87, 783, 1194
TaqII	GACCGA	1 cut(s)	597
TasI	AATT	4 cut(s)	332, 821, 1062, 1436
TatI	WGTACW	2 cut(s)	262, 1214
TauI	GCSGC	2 cut(s)	977, 1240
TfiI	GAWTC	6 cut(s)	57, 640, 869, 1184, 1343, 1353
Tru1I	TTAA	5 cut(s)	249, 290, 518, 660, 899
Tru9I	TTAA	5 cut(s)	249, 290, 518, 660, 899
TscAI	CASTG	3 cut(s)	235, 278, 376
TseFI	GTSAC	3 cut(s)	178, 475, 712
TseI	GCWGC	1 cut(s)	1234
Tsp45I	GTSAC	3 cut(s)	178, 475, 712
TspDTI	ATGAA	6 cut(s)	42, 212, 390, 771, 879, 1145
TspGWI	ACGGA	2 cut(s)	69, 108
TspMI	CCCGGG	1 cut(s)	670
TspRI	CASTG	3 cut(s)	235, 278, 376
Vha464I	CTTAAG	1 cut(s)	289
VpaK11BI	GGWCC	1 cut(s)	803
XagI	CCTNNNNNAGG	1 cut(s)	1142
XapI	RAATTY	1 cut(s)	1436
XmaI	CCCGGG	1 cut(s)	670
XmiI	GTMKAC	2 cut(s)	1194, 1260
XmnI	GAANNNNTTC	1 cut(s)	960
XspI	CTAG	3 cut(s)	278, 354, 1337
Zsp2I	ATGCAT	1 cut(s)	1282

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.