MD17G1035600.v1.1

CLASP N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006098
Physical Location & Seq
Forward (+)
2554818 .. 2558091
3274 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1035600.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGTCAGAGAACGCTCTTAGAGATCTCAATACCATACCTGGATCTGAGAGGAAGAATGAAAGCTCTAGCAAAGGGGGCATGACTAAGCCCGTCGTTGTGAATGCTAATGAAAATCATGAAGAATGCCCGAAGAAAACCACAGCTTCAGTGGGTTCACTTTCTATAAATGGCGAGGAAGTTGCAAATTCCGTACCCGAGGTTGGAATTGCTGAAGTAGAATACATTGAGTCTGAGAATCTGAATGATCTAGAAGATGTTGATACGAGCCTCAAGACACTACTAACTGGACTAGAGTCTAAAGATTGGGTTCTGGTGTGTGAAGCACTCAATAATGTTCGTCGGTTTTCTATATTTCATAAGGAAGCCATGCTTGATATGTTGGGAGATGTGATATCGCTTGTTGTAAAGTCACTGAAGAATCCAAGAAGTGCTGTTTGCAAAACTGCTATAATGACATCTGCAGATATTTTCAATGCCTACAATGATCACGTGATTGATTCTTTGGACCCTATGCTTACACAACTTCTTCTAAAGTCGTCACAAGACAAGCGATTTGTGTGTGAGGCAGCTGAGAGAGCTTTAGTAGCGATGACCACATGGGTTTCCCCTACATTGTTGTTACCAAAGTTGCAACCATATCTGAAGAACAGAAATCCTAGAGTTCGAGCGAAGGCATCAACGTGTTTTTGTCGAAGTGTTCCACGACTGGGTGTTGAAGGTATTAAAGCGTATGGGTTTGACAAATTAATTCAGATCGCTGCATCTCAGCTTAGTGACCAACTTCCTGAGTCAAGGGAGGCTGCTCGGATCCTCCTCCTGGAGCTGCAGACTGTTTACGATAAATTTGATGATCCCCCTCCAGCAGCAGTGTGCGAGCATCCAGAAACGAGCTCAGCAGCAGCGTCTGAGCTCCCAGAAACGAGCTCATGGGAGAACTTTTGTCAGTCAAAACTCTCGCCTCTCAGTGCACAAGCTGTTCTTCGGGTAACCAGTGTCGCTCGGGAAGGTCTTGTTATGGGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLASP_N

Protein Analysis

342

Amino Acids

37.3

Weight (kDa)

5.12

Isoelectric Point (pI)

39.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLASP_N PF12348 122 - 279 4.5e-06 CLASP N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 49, 802, 815, 845
AcsI RAATTY 2 cut(s) 184, 842
AcuI CTGAAG 4 cut(s) 129, 231, 434, 662
AcvI CACGTG 1 cut(s) 490
AfaI GTAC 1 cut(s) 192
AfiI CCNNNNNNNGG 3 cut(s) 200, 707, 817
AflIII ACRYGT 1 cut(s) 680
AgsI TTSAA 2 cut(s) 472, 716
AjnI CCWGG 2 cut(s) 37, 816
Alw21I GWGCWC 4 cut(s) 893, 912, 926, 970
Alw44I GTGCAC 1 cut(s) 966
AlwI GGATC 4 cut(s) 49, 802, 815, 845
AlwNI CAGNNNCTG 1 cut(s) 905
Ama87I CYCGRG 2 cut(s) 194, 999
ApaLI GTGCAC 1 cut(s) 966
ApeKI GCWGC 7 cut(s) 566, 758, 800, 823, 863, 896, 899
ApoI RAATTY 2 cut(s) 184, 842
ArsI GACNNNNNNTTYG 2 cut(s) 536, 568
AseI ATTAAT 1 cut(s) 746
AspS9I GGNCC 1 cut(s) 505
AvaI CYCGRG 2 cut(s) 194, 999
AvaII GGWCC 1 cut(s) 505
BaeGI GKGCMC 1 cut(s) 970
BamHI GGATCC 1 cut(s) 807
BanII GRGCYC 3 cut(s) 893, 912, 926
BbrPI CACGTG 1 cut(s) 490
Bbv12I GWGCWC 4 cut(s) 893, 912, 926, 970
BbvI GCAGC 7 cut(s) 578, 745, 787, 810, 875, 908, 911
BcgI CGANNNNNNTGC 2 cut(s) 161, 195
BciT130I CCWGG 2 cut(s) 39, 818
BclI TGATCA 1 cut(s) 484
BfaI CTAG 4 cut(s) 66, 248, 290, 657
BfmI CTRYAG 2 cut(s) 459, 824
BglII AGATCT 1 cut(s) 22
BisI GCNGC 7 cut(s) 567, 759, 801, 824, 864, 897, 900
BlpI GCTNAGC 1 cut(s) 892
BlsI GCNGC 7 cut(s) 568, 760, 802, 825, 865, 898, 901
Bme1390I CCNGG 2 cut(s) 39, 818
Bme18I GGWCC 1 cut(s) 505
BmeT110I CYCGRG 2 cut(s) 194, 999
BmgT120I GGNCC 1 cut(s) 505
BmiI GGNNCC 2 cut(s) 507, 809
BmrFI CCNGG 2 cut(s) 39, 818
BmrI ACTGGG 1 cut(s) 716
BmsI GCATC 3 cut(s) 683, 770, 886
BmuI ACTGGG 1 cut(s) 716
BoxI GACNNNNGTC 1 cut(s) 292
BpmI CTGGAG 2 cut(s) 839, 843
Bpu1102I GCTNAGC 1 cut(s) 892
BpuEI CTTGAG 1 cut(s) 254
BsaAI YACGTR 1 cut(s) 490
BsaJI CCNNGG 1 cut(s) 195
Bsc4I CCNNNNNNNGG 3 cut(s) 200, 707, 817
Bse1I ACTGG 3 cut(s) 289, 711, 990
BseBI CCWGG 2 cut(s) 39, 818
BseDI CCNNGG 1 cut(s) 195
BseGI GGATG 1 cut(s) 877
BseLI CCNNNNNNNGG 3 cut(s) 200, 707, 817
BseMII CTCAG 8 cut(s) 36, 222, 561, 777, 779, 897, 906, 976
BseNI ACTGG 3 cut(s) 289, 711, 990
BseRI GAGGAG 1 cut(s) 803
BseSI GKGCMC 1 cut(s) 970
BseXI GCAGC 7 cut(s) 578, 745, 787, 810, 875, 908, 911
BsiHKAI GWGCWC 4 cut(s) 893, 912, 926, 970
BsiHKCI CYCGRG 2 cut(s) 194, 999
BslI CCNNNNNNNGG 3 cut(s) 200, 707, 817
BsmI GAATGC 2 cut(s) 106, 128
BsoBI CYCGRG 2 cut(s) 194, 999
Bsp1286I GDGCHC 4 cut(s) 893, 912, 926, 970
Bsp143I GATC 8 cut(s) 22, 41, 244, 484, 753, 807, 850, 1019
Bsp1720I GCTNAGC 1 cut(s) 892
BspCNI CTCAG 8 cut(s) 37, 223, 562, 778, 778, 898, 905, 975
BspHI TCATGA 1 cut(s) 115
BspLI GGNNCC 2 cut(s) 507, 809
BspMAI CTGCAG 2 cut(s) 463, 828
BspPI GGATC 4 cut(s) 49, 802, 815, 845
BsrI ACTGG 3 cut(s) 289, 711, 990
BssECI CCNNGG 1 cut(s) 195
BssMI GATC 8 cut(s) 22, 41, 244, 484, 753, 807, 850, 1019
Bst2UI CCWGG 2 cut(s) 39, 818
Bst4CI ACNGT 1 cut(s) 832
BstBAI YACGTR 1 cut(s) 490
BstC8I GCNNGC 1 cut(s) 875
BstEII GGTNACC 1 cut(s) 985
BstF5I GGATG 1 cut(s) 877
BstKTI GATC 8 cut(s) 25, 44, 247, 487, 756, 810, 853, 1022
BstMBI GATC 8 cut(s) 22, 41, 244, 484, 753, 807, 850, 1019
BstMWI GCNNNNNNNGC 3 cut(s) 75, 575, 584
BstNI CCWGG 2 cut(s) 39, 818
BstPAI GACNNNNGTC 1 cut(s) 292
BstPI GGTNACC 1 cut(s) 985
BstSCI CCNGG 2 cut(s) 37, 816
BstSFI CTRYAG 2 cut(s) 459, 824
BstSLI GKGCMC 1 cut(s) 970
BstV1I GCAGC 7 cut(s) 578, 745, 787, 810, 875, 908, 911
BstX2I RGATCY 4 cut(s) 22, 41, 807, 1019
BstYI RGATCY 4 cut(s) 22, 41, 807, 1019
BtgZI GCGATG 1 cut(s) 602
BtsCI GGATG 1 cut(s) 877
BtsI GCAGTG 1 cut(s) 873
BtsIMutI CAGTG 5 cut(s) 153, 410, 873, 970, 997
Cac8I GCNNGC 1 cut(s) 875
CaiI CAGNNNCTG 1 cut(s) 905
CciI TCATGA 1 cut(s) 115
Cfr13I GGNCC 1 cut(s) 505
CseI GACGC 1 cut(s) 891
Csp6I GTAC 1 cut(s) 191
CviAII CATG 5 cut(s) 79, 116, 367, 597, 927
CviQI GTAC 1 cut(s) 191
DpnI GATC 8 cut(s) 24, 43, 246, 486, 755, 809, 852, 1021
DpnII GATC 8 cut(s) 22, 41, 244, 484, 753, 807, 850, 1019
Ecl136II GAGCTC 3 cut(s) 891, 910, 924
Eco24I GRGCYC 3 cut(s) 893, 912, 926
Eco32I GATATC 1 cut(s) 393
Eco47I GGWCC 1 cut(s) 505
Eco53kI GAGCTC 3 cut(s) 891, 910, 924
Eco57I CTGAAG 4 cut(s) 129, 231, 434, 662
Eco72I CACGTG 1 cut(s) 490
Eco88I CYCGRG 2 cut(s) 194, 999
Eco91I GGTNACC 1 cut(s) 985
EcoICRI GAGCTC 3 cut(s) 891, 910, 924
EcoO65I GGTNACC 1 cut(s) 985
EcoRII CCWGG 2 cut(s) 37, 816
EcoRV GATATC 1 cut(s) 393
EcoT38I GRGCYC 3 cut(s) 893, 912, 926
FaeI CATG 5 cut(s) 82, 119, 370, 600, 930
FalI AAGNNNNNCTT 4 cut(s) 354, 386, 963, 995
FatI CATG 5 cut(s) 78, 115, 366, 596, 926
FbaI TGATCA 1 cut(s) 484
Fnu4HI GCNGC 7 cut(s) 567, 759, 801, 824, 864, 897, 900
FokI GGATG 1 cut(s) 864
FriOI GRGCYC 3 cut(s) 893, 912, 926
Fsp4HI GCNGC 7 cut(s) 567, 759, 801, 824, 864, 897, 900
FspBI CTAG 4 cut(s) 66, 248, 290, 657
GluI GCNGC 7 cut(s) 567, 759, 801, 824, 864, 897, 900
GsuI CTGGAG 2 cut(s) 839, 843
HgaI GACGC 1 cut(s) 891
Hin1II CATG 5 cut(s) 82, 119, 370, 600, 930
HinfI GANTC 6 cut(s) 227, 235, 293, 418, 497, 788
Hpy166II GTNNAC 3 cut(s) 155, 835, 968
Hpy188I TCNGA 8 cut(s) 7, 46, 232, 240, 642, 753, 807, 907
Hpy188III TCNNGA 7 cut(s) 116, 248, 271, 785, 881, 1001, 1023
Hpy8I GTNNAC 3 cut(s) 155, 835, 968
Hpy99I CGWCG 2 cut(s) 95, 342
HpyAV CCTTC 3 cut(s) 664, 710, 998
HpyCH4III ACNGT 1 cut(s) 832
HpyCH4IV ACGT 2 cut(s) 489, 680
HpyCH4V TGCA 7 cut(s) 182, 438, 461, 631, 761, 826, 968
HpyF10VI GCNNNNNNNGC 3 cut(s) 75, 575, 584
HpySE526I ACGT 2 cut(s) 489, 680
Hsp92II CATG 5 cut(s) 82, 119, 370, 600, 930
Ksp22I TGATCA 1 cut(s) 484
Kzo9I GATC 8 cut(s) 22, 41, 244, 484, 753, 807, 850, 1019
LmnI GCTCC 2 cut(s) 820, 915
Lsp1109I GCAGC 7 cut(s) 578, 745, 787, 810, 875, 908, 911
LweI GCATC 3 cut(s) 683, 770, 886
MaeI CTAG 4 cut(s) 66, 248, 290, 657
MaeII ACGT 2 cut(s) 489, 680
MaeIII GTNAC 5 cut(s) 408, 537, 618, 773, 985
MalI GATC 8 cut(s) 24, 43, 246, 486, 755, 809, 852, 1021
MboI GATC 8 cut(s) 22, 41, 244, 484, 753, 807, 850, 1019
MboII GAAGA 8 cut(s) 64, 131, 142, 263, 427, 518, 655, 971
MflI RGATCY 4 cut(s) 22, 41, 807, 1019
MhlI GDGCHC 4 cut(s) 893, 912, 926, 970
MluCI AATT 5 cut(s) 184, 204, 743, 747, 842
MlyI GAGTC 3 cut(s) 236, 302, 797
MmeI TCCRAC 1 cut(s) 181
MseI TTAA 2 cut(s) 723, 746
MslI CAYNNNNRTG 1 cut(s) 679
MspA1I CMGCKG 1 cut(s) 569
MspR9I CCNGG 2 cut(s) 39, 818
Mva1269I GAATGC 2 cut(s) 106, 128
MvaI CCWGG 2 cut(s) 39, 818
MwoI GCNNNNNNNGC 3 cut(s) 75, 575, 584
NdeII GATC 8 cut(s) 22, 41, 244, 484, 753, 807, 850, 1019
NlaIII CATG 5 cut(s) 82, 119, 370, 600, 930
NlaIV GGNNCC 2 cut(s) 507, 809
NmuCI GTSAC 3 cut(s) 408, 537, 773
PagI TCATGA 1 cut(s) 115
PctI GAATGC 2 cut(s) 106, 128
PfeI GAWTC 3 cut(s) 235, 418, 497
PfoI TCCNGGA 1 cut(s) 816
PkrI GCNGC 7 cut(s) 568, 760, 802, 825, 865, 898, 901
PleI GAGTC 3 cut(s) 235, 301, 796
PmaCI CACGTG 1 cut(s) 490
PmlI CACGTG 1 cut(s) 490
PpsI GAGTC 3 cut(s) 235, 301, 796
Ppu21I YACGTR 1 cut(s) 490
PshAI GACNNNNGTC 1 cut(s) 292
PshBI ATTAAT 1 cut(s) 746
Psp124BI GAGCTC 3 cut(s) 893, 912, 926
Psp6I CCWGG 2 cut(s) 37, 816
PspCI CACGTG 1 cut(s) 490
PspEI GGTNACC 1 cut(s) 985
PspGI CCWGG 2 cut(s) 37, 816
PspN4I GGNNCC 2 cut(s) 507, 809
PspPI GGNCC 1 cut(s) 505
PstI CTGCAG 2 cut(s) 463, 828
PstNI CAGNNNCTG 1 cut(s) 905
PsuI RGATCY 4 cut(s) 22, 41, 807, 1019
PvuII CAGCTG 1 cut(s) 569
RsaI GTAC 1 cut(s) 192
RsaNI GTAC 1 cut(s) 191
RseI CAYNNNNRTG 1 cut(s) 679
SacI GAGCTC 3 cut(s) 893, 912, 926
SaqAI TTAA 2 cut(s) 723, 746
SatI GCNGC 7 cut(s) 567, 759, 801, 824, 864, 897, 900
Sau3AI GATC 8 cut(s) 22, 41, 244, 484, 753, 807, 850, 1019
Sau96I GGNCC 1 cut(s) 505
SchI GAGTC 3 cut(s) 236, 302, 797
ScrFI CCNGG 2 cut(s) 39, 818
SduI GDGCHC 4 cut(s) 893, 912, 926, 970
SfaNI GCATC 3 cut(s) 683, 770, 886
SfcI CTRYAG 2 cut(s) 459, 824
SinI GGWCC 1 cut(s) 505
SmiMI CAYNNNNRTG 1 cut(s) 679
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
Sse9I AATT 5 cut(s) 184, 204, 743, 747, 842
SspMI CTAG 4 cut(s) 66, 248, 290, 657
SstI GAGCTC 3 cut(s) 893, 912, 926
StyD4I CCNGG 2 cut(s) 37, 816
TaaI ACNGT 1 cut(s) 832
TaiI ACGT 2 cut(s) 492, 683
TaqI TCGA 2 cut(s) 664, 691
TasI AATT 5 cut(s) 184, 204, 743, 747, 842
TfiI GAWTC 3 cut(s) 235, 418, 497
Tru1I TTAA 2 cut(s) 723, 746
Tru9I TTAA 2 cut(s) 723, 746
TscAI CASTG 5 cut(s) 153, 417, 873, 970, 997
TseFI GTSAC 3 cut(s) 408, 537, 773
TseI GCWGC 7 cut(s) 566, 758, 800, 823, 863, 896, 899
Tsp45I GTSAC 3 cut(s) 408, 537, 773
TspDTI ATGAA 4 cut(s) 72, 123, 132, 344
TspGWI ACGGA 1 cut(s) 178
TspRI CASTG 5 cut(s) 153, 417, 873, 970, 997
VneI GTGCAC 1 cut(s) 966
VpaK11BI GGWCC 1 cut(s) 505
VspI ATTAAT 1 cut(s) 746
XapI RAATTY 2 cut(s) 184, 842
XbaI TCTAGA 1 cut(s) 247
XcmI CCANNNNNNNNNTGG 1 cut(s) 145
XspI CTAG 4 cut(s) 66, 248, 290, 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.