Rorug02G0553400

CLASP N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006098
Physical Location & Seq
Reverse (-)
68044512 .. 68063870
19359 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0553400.1

Sequence Viewer

Length: 468 bp
ATGGCGCTTGGAAGAGTTGGAAGAAGCTTCATGGCTCCTATTTTAGCAGTCAACCTGGTGGTTCATCTGATCGTGCTTGGATTGGCTGGTTGGTCACTTGATAAGTACATCGACGGCGAACAAAATCACCCACATTTGGGAGGGAATACATCGACTAACTTTATGTTAATCTTTGCTCTAATTGCCGGAGCGTTTGGTGCTTGCTCTGTAATTATAGGGATGATGCATCTGCAAGCATGGCAGCACGAAAGTCATGCTGCTGCAGCTTCTTTAGCCATTATTTCCTGGGCTATGATAGCCCTGGCTTTCGGTTTTGTATGTAAGGAGGTCATATTAGGGGGACACAGAGGAAACCGATTGCAAACATTGGAACTTTTAATTGCAATATCACTACTAAGTCAATTGCTGTACTTGGTGCTACTGAATGCTGGGCTGTTCAAGAGGAGATATGGACCGACTGTTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLASP_N

Protein Analysis

155

Amino Acids

16.69

Weight (kDa)

9.18

Isoelectric Point (pI)

30.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AWPM-19 PF05512 17 - 144 6.5e-37 AWPM-19-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 2 cut(s) 107, 410
AfiI CCNNNNNNNGG 2 cut(s) 136, 137
AgsI TTSAA 1 cut(s) 439
AjnI CCWGG 3 cut(s) 54, 284, 300
AluBI AGCT 2 cut(s) 27, 266
AluI AGCT 2 cut(s) 27, 266
ApeKI GCWGC 4 cut(s) 241, 257, 260, 263
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 452
AsuHPI GGTGA 1 cut(s) 119
AvaII GGWCC 1 cut(s) 452
BbvI GCAGC 4 cut(s) 244, 247, 253, 275
BceAI ACGGC 1 cut(s) 130
BcgI CGANNNNNNTGC 2 cut(s) 236, 270
BciT130I CCWGG 3 cut(s) 56, 286, 302
BfmI CTRYAG 1 cut(s) 261
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 4 cut(s) 242, 258, 261, 264
BlsI GCNGC 4 cut(s) 243, 259, 262, 265
Bme1390I CCNGG 3 cut(s) 56, 286, 302
Bme18I GGWCC 1 cut(s) 452
BmgT120I GGNCC 1 cut(s) 452
BmiI GGNNCC 1 cut(s) 36
BmrFI CCNGG 3 cut(s) 56, 286, 302
BmsI GCATC 2 cut(s) 213, 235
BoxI GACNNNNGTC 1 cut(s) 461
BsaJI CCNNGG 2 cut(s) 285, 300
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 137
BseBI CCWGG 3 cut(s) 56, 286, 302
BseDI CCNNGG 2 cut(s) 285, 300
BseGI GGATG 1 cut(s) 225
BseLI CCNNNNNNNGG 2 cut(s) 136, 137
BseRI GAGGAG 1 cut(s) 457
BseXI GCAGC 4 cut(s) 244, 247, 253, 275
BseYI CCCAGC 1 cut(s) 428
BsiSI CCGG 1 cut(s) 186
BslFI GGGAC 1 cut(s) 354
BslI CCNNNNNNNGG 2 cut(s) 136, 137
BsmFI GGGAC 1 cut(s) 354
BsmI GAATGC 1 cut(s) 430
Bsp143I GATC 1 cut(s) 69
BspLI GGNNCC 1 cut(s) 36
BspMAI CTGCAG 1 cut(s) 265
BssECI CCNNGG 2 cut(s) 285, 300
BssMI GATC 1 cut(s) 69
Bst2UI CCWGG 3 cut(s) 56, 286, 302
Bst4CI ACNGT 1 cut(s) 460
Bst6I CTCTTC 1 cut(s) 7
BstC8I GCNNGC 2 cut(s) 202, 234
BstDEI CTNAG 1 cut(s) 395
BstF5I GGATG 1 cut(s) 225
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 1 cut(s) 72
BstMBI GATC 1 cut(s) 69
BstMWI GCNNNNNNNGC 6 cut(s) 182, 197, 238, 263, 272, 296
BstNI CCWGG 3 cut(s) 56, 286, 302
BstPAI GACNNNNGTC 1 cut(s) 461
BstSCI CCNGG 3 cut(s) 54, 284, 300
BstSFI CTRYAG 1 cut(s) 261
BstV1I GCAGC 4 cut(s) 244, 247, 253, 275
BtsCI GGATG 1 cut(s) 225
Cac8I GCNNGC 2 cut(s) 202, 234
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 452
CsiI ACCWGGT 1 cut(s) 54
Csp6I GTAC 2 cut(s) 106, 409
CviAII CATG 3 cut(s) 31, 237, 254
CviJI RGCY 9 cut(s) 27, 35, 86, 266, 275, 290, 299, 305, 433
CviKI_1 RGCY 9 cut(s) 27, 35, 86, 266, 275, 290, 299, 305, 433
CviQI GTAC 2 cut(s) 106, 409
DdeI CTNAG 1 cut(s) 395
DpnI GATC 1 cut(s) 71
DpnII GATC 1 cut(s) 69
Eam1104I CTCTTC 1 cut(s) 7
EarI CTCTTC 1 cut(s) 7
Eco47I GGWCC 1 cut(s) 452
EcoRII CCWGG 3 cut(s) 54, 284, 300
EcoT22I ATGCAT 1 cut(s) 228
FaeI CATG 3 cut(s) 34, 240, 257
FaiI YATR 9 cut(s) 32, 164, 215, 238, 255, 293, 319, 332, 450
FaqI GGGAC 1 cut(s) 354
FatI CATG 3 cut(s) 30, 236, 253
Fnu4HI GCNGC 4 cut(s) 242, 258, 261, 264
FokI GGATG 1 cut(s) 232
Fsp4HI GCNGC 4 cut(s) 242, 258, 261, 264
GlaI GCGC 1 cut(s) 6
GluI GCNGC 4 cut(s) 242, 258, 261, 264
GsaI CCCAGC 1 cut(s) 432
HaeII RGCGCY 1 cut(s) 8
HapII CCGG 1 cut(s) 186
HhaI GCGC 1 cut(s) 7
Hin1II CATG 3 cut(s) 34, 240, 257
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HindIII AAGCTT 1 cut(s) 25
HpaII CCGG 1 cut(s) 186
HphI GGTGA 1 cut(s) 119
Hpy166II GTNNAC 1 cut(s) 52
Hpy188I TCNGA 2 cut(s) 69, 467
Hpy188III TCNNGA 1 cut(s) 439
Hpy8I GTNNAC 1 cut(s) 52
Hpy99I CGWCG 1 cut(s) 116
HpyCH4III ACNGT 1 cut(s) 460
HpyCH4V TGCA 5 cut(s) 226, 232, 263, 361, 383
HpyF10VI GCNNNNNNNGC 6 cut(s) 182, 197, 238, 263, 272, 296
HpyF3I CTNAG 1 cut(s) 395
Hsp92II CATG 3 cut(s) 34, 240, 257
HspAI GCGC 1 cut(s) 5
Kzo9I GATC 1 cut(s) 69
LmnI GCTCC 2 cut(s) 40, 188
LpnPI CCDG 9 cut(s) 41, 68, 72, 199, 271, 287, 298, 314, 414
Lsp1109I GCAGC 4 cut(s) 244, 247, 253, 275
LweI GCATC 2 cut(s) 213, 235
MabI ACCWGGT 1 cut(s) 54
MaeIII GTNAC 1 cut(s) 93
MalI GATC 1 cut(s) 71
MboI GATC 1 cut(s) 69
MboII GAAGA 2 cut(s) 24, 33
MfeI CAATTG 1 cut(s) 401
MluCI AATT 4 cut(s) 180, 210, 378, 401
MnlI CCTC 4 cut(s) 134, 319, 341, 435
Mph1103I ATGCAT 1 cut(s) 228
MseI TTAA 2 cut(s) 167, 377
MspI CCGG 1 cut(s) 186
MspR9I CCNGG 3 cut(s) 56, 286, 302
MunI CAATTG 1 cut(s) 401
Mva1269I GAATGC 1 cut(s) 430
MvaI CCWGG 3 cut(s) 56, 286, 302
MwoI GCNNNNNNNGC 6 cut(s) 182, 197, 238, 263, 272, 296
NdeII GATC 1 cut(s) 69
NlaIII CATG 3 cut(s) 34, 240, 257
NlaIV GGNNCC 1 cut(s) 36
NmuCI GTSAC 1 cut(s) 93
NsiI ATGCAT 1 cut(s) 228
PctI GAATGC 1 cut(s) 430
PkrI GCNGC 4 cut(s) 243, 259, 262, 265
PshAI GACNNNNGTC 1 cut(s) 461
Psp6I CCWGG 3 cut(s) 54, 284, 300
PspFI CCCAGC 1 cut(s) 428
PspGI CCWGG 3 cut(s) 54, 284, 300
PspN4I GGNNCC 1 cut(s) 36
PspPI GGNCC 1 cut(s) 452
PstI CTGCAG 1 cut(s) 265
RsaI GTAC 2 cut(s) 107, 410
RsaNI GTAC 2 cut(s) 106, 409
SaqAI TTAA 2 cut(s) 167, 377
SatI GCNGC 4 cut(s) 242, 258, 261, 264
Sau3AI GATC 1 cut(s) 69
Sau96I GGNCC 1 cut(s) 452
ScrFI CCNGG 3 cut(s) 56, 286, 302
SetI ASST 4 cut(s) 29, 57, 268, 330
SexAI ACCWGGT 1 cut(s) 54
SfaNI GCATC 2 cut(s) 213, 235
SfcI CTRYAG 1 cut(s) 261
SinI GGWCC 1 cut(s) 452
Sse9I AATT 4 cut(s) 180, 210, 378, 401
StyD4I CCNGG 3 cut(s) 54, 284, 300
TaaI ACNGT 1 cut(s) 460
TaqI TCGA 2 cut(s) 111, 152
TasI AATT 4 cut(s) 180, 210, 378, 401
TatI WGTACW 2 cut(s) 105, 408
Tru1I TTAA 2 cut(s) 167, 377
Tru9I TTAA 2 cut(s) 167, 377
TseFI GTSAC 1 cut(s) 93
TseI GCWGC 4 cut(s) 241, 257, 260, 263
Tsp45I GTSAC 1 cut(s) 93
TspDTI ATGAA 2 cut(s) 19, 53
VpaK11BI GGWCC 1 cut(s) 452
Zsp2I ATGCAT 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.