RLG00000022000

CLASP N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006098
Physical Location & Seq
Forward (+)
79849372 .. 79851613
2242 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022000

Sequence Viewer

Length: 1011 bp
ATGTCAGCTCTTAGAGATCTCAATACCATACCTGGATCTGAGAGGAAGAATGAAAGCTGTAGCAAAGGGGTCCTAACTAAACCCTTTGCTGGGATTGCTACTGAAAATCATGAAGAGTGCCAGAAGAAAAACTCTGTCTCTTTGGGTGCCCCTTCTACTAATGGTACCCCTTCTACTAATGGTGAGGAAGTTGTGAATGCCGTTGAGGTTGCAAATACAGAAGTAGAATACATTGAGTCTGAGAATTTGAATGATGTAGAAGATGTTGATTTGAGTCTCAAGACGCTCTTAACTGGACTAGACTCTAAAGATTGGATGCTGCTTTGTGAAGCACTCAATACCGTCCGTCGGTTATCCATTTTTCACAAGGAAGCTATGCTCGATACACTGGGAGATGTGATTCCACTTGTAGTAAGGTCCCTGAAGAATCCAAGAAGTGCTGTTTGCAAAACTGCAATTATGACATCTGCAGATATCTTCAACGCTTACAATGACAGTGTAGTTGATTCATTGGACCCTCTGCTACTACAACTTCTTCTCAAGTCTTCACAAGACAAACGATTTGTATGCGAGGCAGCTGAAAGAGCTTTAGTAGCAATGACTACATGTGTTTCCCCCACATTGTTGTTACCAATGCTGCAACCATATCTCAAGCACAAAAATCCTAGAATTCGAGCGAAGGCATCAATGTGCTTTTGTCGAAGTGTTCCACGACTGGGTGTTGAAGGTACTAAAGCATATGGGATTGACAAATTAATTCAAGTTGCCGCCTCCCAGCTTAGTGACCAGCTTCCTGAATCAAGGGAGGCTGCTCGAAGCCTCCTCCTTGAGTTGCAGACTGTTTATGGGAAATTTCAGAATCTCTCTCCAACAGCAGTATCTGAGGAGATGCAACCAGAGGTGGACTCTTGGGAGAACTTCTGCCAATCGAAACTCTCTCCGCTTAGCGCACAAGCTGTTCTTCGGGTGACCAATATCTCTCGGCAAGGTCTTGTTGTTGGCTTCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLASP_N

Protein Analysis

337

Amino Acids

36.78

Weight (kDa)

5.35

Isoelectric Point (pI)

42.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLASP_N PF12348 126 - 282 2.6e-06 CLASP N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 164
AccB1I GGYRCC 2 cut(s) 146, 164
AciI CCGC 2 cut(s) 768, 941
AclWI GGATC 1 cut(s) 43
AcsI RAATTY 3 cut(s) 244, 669, 851
AcuI CTGAAG 1 cut(s) 443
AfaI GTAC 2 cut(s) 166, 730
AfiI CCNNNNNNNGG 4 cut(s) 89, 90, 348, 716
AflIII ACRYGT 1 cut(s) 605
AgsI TTSAA 4 cut(s) 250, 481, 725, 761
AjnI CCWGG 1 cut(s) 31
AluBI AGCT 8 cut(s) 8, 57, 374, 578, 587, 778, 790, 956
AluI AGCT 8 cut(s) 8, 57, 374, 578, 587, 778, 790, 956
Alw26I GTCTC 2 cut(s) 142, 281
AlwI GGATC 1 cut(s) 43
AlwNI CAGNNNCTG 1 cut(s) 881
ApeKI GCWGC 4 cut(s) 319, 575, 637, 809
ApoI RAATTY 3 cut(s) 244, 669, 851
ArsI GACNNNNNNTTYG 2 cut(s) 545, 577
AseI ATTAAT 1 cut(s) 755
Asp718I GGTACC 1 cut(s) 164
AspLEI GCGC 1 cut(s) 950
AspS9I GGNCC 3 cut(s) 70, 417, 514
AsuHPI GGTGA 2 cut(s) 194, 979
AvaII GGWCC 3 cut(s) 70, 417, 514
BaeGI GKGCMC 1 cut(s) 151
BaeI ACNNNNGTAYC 2 cut(s) 861, 894
BanI GGYRCC 2 cut(s) 146, 164
BbsI GAAGAC 1 cut(s) 537
BbvI GCAGC 4 cut(s) 306, 587, 624, 796
BceAI ACGGC 1 cut(s) 185
BcgI CGANNNNNNTGC 2 cut(s) 549, 583
BciT130I CCWGG 1 cut(s) 33
BcoDI GTCTC 2 cut(s) 142, 281
BfaI CTAG 2 cut(s) 299, 666
BfmI CTRYAG 2 cut(s) 58, 468
BglII AGATCT 1 cut(s) 16
BisI GCNGC 5 cut(s) 320, 576, 638, 768, 810
BlpI GCTNAGC 1 cut(s) 944
BlsI GCNGC 5 cut(s) 321, 577, 639, 769, 811
Bme1390I CCNGG 1 cut(s) 33
Bme18I GGWCC 3 cut(s) 70, 417, 514
BmgT120I GGNCC 3 cut(s) 70, 417, 514
BmiI GGNNCC 5 cut(s) 71, 148, 166, 419, 516
BmrFI CCNGG 1 cut(s) 33
BmrI ACTGGG 2 cut(s) 398, 725
BmsI GCATC 3 cut(s) 306, 692, 879
BmuI ACTGGG 2 cut(s) 398, 725
BpiI GAAGAC 1 cut(s) 537
BplI GAGNNNNNCTC 2 cut(s) 890, 922
Bpu1102I GCTNAGC 1 cut(s) 944
BpuEI CTTGAG 4 cut(s) 263, 524, 635, 848
Bsc4I CCNNNNNNNGG 4 cut(s) 89, 90, 348, 716
Bse1I ACTGG 3 cut(s) 298, 393, 720
Bse3DI GCAATG 1 cut(s) 603
BseBI CCWGG 1 cut(s) 33
BseGI GGATG 1 cut(s) 321
BseLI CCNNNNNNNGG 4 cut(s) 89, 90, 348, 716
BseMI GCAATG 1 cut(s) 603
BseMII CTCAG 3 cut(s) 30, 231, 873
BseNI ACTGG 3 cut(s) 298, 393, 720
BseRI GAGGAG 2 cut(s) 812, 899
BseSI GKGCMC 1 cut(s) 151
BseXI GCAGC 4 cut(s) 306, 587, 624, 796
BseYI CCCAGC 2 cut(s) 89, 774
BshNI GGYRCC 2 cut(s) 146, 164
BslFI GGGAC 1 cut(s) 403
BslI CCNNNNNNNGG 4 cut(s) 89, 90, 348, 716
BsmAI GTCTC 2 cut(s) 142, 281
BsmFI GGGAC 1 cut(s) 403
BsmI GAATGC 1 cut(s) 202
Bsp1286I GDGCHC 1 cut(s) 151
Bsp143I GATC 2 cut(s) 16, 35
Bsp1720I GCTNAGC 1 cut(s) 944
BspACI CCGC 2 cut(s) 768, 941
BspCNI CTCAG 3 cut(s) 31, 232, 874
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 5 cut(s) 71, 148, 166, 419, 516
BspMAI CTGCAG 1 cut(s) 472
BspPI GGATC 1 cut(s) 43
BspT107I GGYRCC 2 cut(s) 146, 164
BsrDI GCAATG 1 cut(s) 603
BsrI ACTGG 3 cut(s) 298, 393, 720
BssMI GATC 2 cut(s) 16, 35
Bst2UI CCWGG 1 cut(s) 33
Bst4CI ACNGT 3 cut(s) 343, 497, 841
Bst6I CTCTTC 1 cut(s) 108
BstDEI CTNAG 6 cut(s) 11, 39, 240, 779, 882, 944
BstEII GGTNACC 1 cut(s) 967
BstF5I GGATG 1 cut(s) 321
BstHHI GCGC 1 cut(s) 950
BstKTI GATC 2 cut(s) 19, 38
BstMAI GTCTC 2 cut(s) 142, 281
BstMBI GATC 2 cut(s) 16, 35
BstMWI GCNNNNNNNGC 3 cut(s) 95, 584, 593
BstNI CCWGG 1 cut(s) 33
BstNSI RCATGY 1 cut(s) 609
BstPI GGTNACC 1 cut(s) 967
BstSCI CCNGG 1 cut(s) 31
BstSFI CTRYAG 2 cut(s) 58, 468
BstSLI GKGCMC 1 cut(s) 151
BstV1I GCAGC 4 cut(s) 306, 587, 624, 796
BstV2I GAAGAC 1 cut(s) 537
BstX2I RGATCY 2 cut(s) 16, 35
BstYI RGATCY 2 cut(s) 16, 35
BtsCI GGATG 1 cut(s) 321
BtsIMutI CAGTG 2 cut(s) 386, 502
CaiI CAGNNNCTG 1 cut(s) 881
CciI TCATGA 1 cut(s) 109
CfoI GCGC 1 cut(s) 950
Cfr13I GGNCC 3 cut(s) 70, 417, 514
CseI GACGC 1 cut(s) 292
Csp6I GTAC 2 cut(s) 165, 729
CviAII CATG 2 cut(s) 110, 606
CviQI GTAC 2 cut(s) 165, 729
DdeI CTNAG 6 cut(s) 11, 39, 240, 779, 882, 944
DpnI GATC 2 cut(s) 18, 37
DpnII GATC 2 cut(s) 16, 35
Eam1104I CTCTTC 1 cut(s) 108
EarI CTCTTC 1 cut(s) 108
Eco32I GATATC 1 cut(s) 475
Eco47I GGWCC 3 cut(s) 70, 417, 514
Eco57I CTGAAG 1 cut(s) 443
Eco91I GGTNACC 1 cut(s) 967
EcoO109I RGGNCCY 2 cut(s) 70, 417
EcoO65I GGTNACC 1 cut(s) 967
EcoRI GAATTC 1 cut(s) 669
EcoRII CCWGG 1 cut(s) 31
EcoRV GATATC 1 cut(s) 475
FaeI CATG 2 cut(s) 113, 609
FalI AAGNNNNNCTT 4 cut(s) 272, 304, 945, 977
FaqI GGGAC 1 cut(s) 403
FatI CATG 2 cut(s) 109, 605
FauNDI CATATG 1 cut(s) 739
Fnu4HI GCNGC 5 cut(s) 320, 576, 638, 768, 810
FokI GGATG 1 cut(s) 328
Fsp4HI GCNGC 5 cut(s) 320, 576, 638, 768, 810
FspBI CTAG 2 cut(s) 299, 666
GlaI GCGC 1 cut(s) 949
GluI GCNGC 5 cut(s) 320, 576, 638, 768, 810
GsaI CCCAGC 2 cut(s) 93, 778
HgaI GACGC 1 cut(s) 292
HhaI GCGC 1 cut(s) 950
Hin1II CATG 2 cut(s) 113, 609
Hin6I GCGC 1 cut(s) 948
HinP1I GCGC 1 cut(s) 948
HinfI GANTC 9 cut(s) 236, 274, 302, 400, 427, 506, 797, 859, 905
HphI GGTGA 2 cut(s) 194, 979
Hpy166II GTNNAC 1 cut(s) 904
Hpy188I TCNGA 4 cut(s) 40, 241, 858, 883
Hpy188III TCNNGA 4 cut(s) 110, 280, 794, 1008
Hpy8I GTNNAC 1 cut(s) 904
Hpy99I CGWCG 1 cut(s) 351
HpyAV CCTTC 4 cut(s) 162, 180, 673, 719
HpyCH4III ACNGT 3 cut(s) 343, 497, 841
HpyCH4V TGCA 7 cut(s) 212, 447, 455, 470, 640, 835, 892
HpyF10VI GCNNNNNNNGC 3 cut(s) 95, 584, 593
HpyF3I CTNAG 6 cut(s) 11, 39, 240, 779, 882, 944
Hsp92II CATG 2 cut(s) 113, 609
HspAI GCGC 1 cut(s) 948
KpnI GGTACC 1 cut(s) 168
Kzo9I GATC 2 cut(s) 16, 35
Lsp1109I GCAGC 4 cut(s) 306, 587, 624, 796
LweI GCATC 3 cut(s) 306, 692, 879
MaeI CTAG 2 cut(s) 299, 666
MaeIII GTNAC 3 cut(s) 627, 782, 967
MalI GATC 2 cut(s) 18, 37
MboI GATC 2 cut(s) 16, 35
MboII GAAGA 9 cut(s) 58, 125, 136, 272, 436, 469, 527, 537, 953
MflI RGATCY 2 cut(s) 16, 35
MhlI GDGCHC 1 cut(s) 151
MluCI AATT 6 cut(s) 244, 456, 669, 752, 756, 851
MlyI GAGTC 4 cut(s) 245, 283, 296, 899
MmeI TCCRAC 1 cut(s) 893
MseI TTAA 2 cut(s) 290, 755
MslI CAYNNNNRTG 1 cut(s) 688
MspA1I CMGCKG 1 cut(s) 578
MspR9I CCNGG 1 cut(s) 33
Mva1269I GAATGC 1 cut(s) 202
MvaI CCWGG 1 cut(s) 33
MwoI GCNNNNNNNGC 3 cut(s) 95, 584, 593
NdeI CATATG 1 cut(s) 739
NdeII GATC 2 cut(s) 16, 35
NlaIII CATG 2 cut(s) 113, 609
NlaIV GGNNCC 5 cut(s) 71, 148, 166, 419, 516
NmeAIII GCCGAG 1 cut(s) 961
NmuCI GTSAC 2 cut(s) 782, 967
NspI RCATGY 1 cut(s) 609
PagI TCATGA 1 cut(s) 109
PciI ACATGT 1 cut(s) 605
PctI GAATGC 1 cut(s) 202
PfeI GAWTC 5 cut(s) 400, 427, 506, 797, 859
PkrI GCNGC 5 cut(s) 321, 577, 639, 769, 811
PleI GAGTC 4 cut(s) 244, 282, 296, 899
PpsI GAGTC 4 cut(s) 244, 282, 296, 899
PpuMI RGGWCCY 2 cut(s) 70, 417
PscI ACATGT 1 cut(s) 605
PshBI ATTAAT 1 cut(s) 755
Psp5II RGGWCCY 2 cut(s) 70, 417
Psp6I CCWGG 1 cut(s) 31
PspEI GGTNACC 1 cut(s) 967
PspFI CCCAGC 2 cut(s) 89, 774
PspGI CCWGG 1 cut(s) 31
PspN4I GGNNCC 5 cut(s) 71, 148, 166, 419, 516
PspPI GGNCC 3 cut(s) 70, 417, 514
PspPPI RGGWCCY 2 cut(s) 70, 417
PstI CTGCAG 1 cut(s) 472
PstNI CAGNNNCTG 1 cut(s) 881
PsuI RGATCY 2 cut(s) 16, 35
PvuII CAGCTG 1 cut(s) 578
RsaI GTAC 2 cut(s) 166, 730
RsaNI GTAC 2 cut(s) 165, 729
RseI CAYNNNNRTG 1 cut(s) 688
SaqAI TTAA 2 cut(s) 290, 755
SatI GCNGC 5 cut(s) 320, 576, 638, 768, 810
Sau3AI GATC 2 cut(s) 16, 35
Sau96I GGNCC 3 cut(s) 70, 417, 514
SchI GAGTC 4 cut(s) 245, 283, 296, 899
ScrFI CCNGG 1 cut(s) 33
SduI GDGCHC 1 cut(s) 151
SfaNI GCATC 3 cut(s) 306, 692, 879
SfcI CTRYAG 2 cut(s) 58, 468
SinI GGWCC 3 cut(s) 70, 417, 514
SmiMI CAYNNNNRTG 1 cut(s) 688
SmlI CTYRAG 4 cut(s) 278, 539, 650, 827
SmoI CTYRAG 4 cut(s) 278, 539, 650, 827
Sse9I AATT 6 cut(s) 244, 456, 669, 752, 756, 851
SsiI CCGC 2 cut(s) 768, 941
SspMI CTAG 2 cut(s) 299, 666
StyD4I CCNGG 1 cut(s) 31
TaaI ACNGT 3 cut(s) 343, 497, 841
TaqI TCGA 5 cut(s) 381, 673, 700, 814, 929
TasI AATT 6 cut(s) 244, 456, 669, 752, 756, 851
TauI GCSGC 1 cut(s) 770
TfiI GAWTC 5 cut(s) 400, 427, 506, 797, 859
Tru1I TTAA 2 cut(s) 290, 755
Tru9I TTAA 2 cut(s) 290, 755
TscAI CASTG 2 cut(s) 393, 502
TseFI GTSAC 2 cut(s) 782, 967
TseI GCWGC 4 cut(s) 319, 575, 637, 809
Tsp45I GTSAC 2 cut(s) 782, 967
TspDTI ATGAA 3 cut(s) 66, 126, 498
TspGWI ACGGA 1 cut(s) 335
TspRI CASTG 2 cut(s) 393, 502
VpaK11BI GGWCC 3 cut(s) 70, 417, 514
VspI ATTAAT 1 cut(s) 755
XapI RAATTY 3 cut(s) 244, 669, 851
XceI RCATGY 1 cut(s) 609
XspI CTAG 2 cut(s) 299, 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.