RchiOBHm_Chr2g0171061

CLASP N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006098
Physical Location & Seq
Forward (+)
84764752 .. 84769176
4425 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53852

Sequence Viewer

Length: 993 bp
ATGTCAGCTCTTAGAGATCTCAATACCATACCTGGATCTGAGAGGAAGAATGAAAGCTGTAGCAAAGGGGTCCTAACTAAACCCTTTGCTGGGATTGCTACCGAAAATCATGAAGAGTGCCAGAAGAAAAACTCTGTCTCTTTGGGTGCCCCTTCTACTAATGGTGAGGAAGTTGTGAATGCAGTTGAGGTTGCAAATACAGAAGTAGAATACATTGAGTCTGAGAACTTGAATGATGTAGAAGATGTTGATTTGAGTCTCAAGACACTCTTAACTGGACTAGAGTCTAAAGATTGGGTGCTGCTTTGTGAAGCACTCAATACTGTCCGTCGGTTATCCATTTTTCACAAGGAAGCTATGCTCGATACACTGGGAGATGTGATTCCACTTGTAGTAAGGTCCCTGAAGAATCCAAGAAGTGCTGTTTGCAAAACTGCAATTATGACATCTGCAGATATCTTCAACGCTTACAATGACAGTATAGTTGATTCATTGGACCCTCTGCTACTGCAACTTCTTCTCAAGTCTTCACAAGACAAACGATTTGTATGCGAGGCAGCTGAAAGAGCTTTAGTAGCAATGACTACATGGGTTTCCCCCACATTGTTATTACCAATGCTGCAACCATATCTCAAGCACAAAAATCCTAGAATTCGAGCGAAGGCATCAATGTGCTTTTGTCGAAGTGTTCCACGACTGGGTGTTGAAGGTACTAAAGCATATGGGATTGACAAATTAATTCAAGTTGCCGCCTCCCAGCTTAGTGACCAGCTTCCTGAATCAAGGGAGGCTGCTCGAAGCCTCCTCCTTGAGTTGCAGACTGTTTATGAGAAATTTCAGAATCTCTCTCCAACAGCAGTATCTGAGGAGATGCAACCAGAGGTGGACTCTTGGGAGAACTTCTGCCAATCGAAACTATCTCCGCTTAGCGCACAAGCTGTTCTTCGGGTGACCAATATCTCTCGGGAAGGTCTTGTTGTTGGCTCCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLASP_N

Protein Analysis

330

Amino Acids

36.31

Weight (kDa)

5.18

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLASP_N PF12348 120 - 276 5.3e-06 CLASP N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 146
AciI CCGC 2 cut(s) 750, 923
AclWI GGATC 1 cut(s) 43
AcsI RAATTY 2 cut(s) 651, 833
AcuI CTGAAG 1 cut(s) 425
AfaI GTAC 1 cut(s) 712
AfiI CCNNNNNNNGG 3 cut(s) 89, 90, 698
AgsI TTSAA 4 cut(s) 232, 463, 707, 743
AjnI CCWGG 1 cut(s) 31
AluBI AGCT 8 cut(s) 8, 57, 356, 560, 569, 760, 772, 938
AluI AGCT 8 cut(s) 8, 57, 356, 560, 569, 760, 772, 938
Alw26I GTCTC 2 cut(s) 142, 263
AlwI GGATC 1 cut(s) 43
AlwNI CAGNNNCTG 1 cut(s) 863
Ama87I CYCGRG 1 cut(s) 963
ApeKI GCWGC 4 cut(s) 301, 557, 619, 791
ApoI RAATTY 2 cut(s) 651, 833
ArsI GACNNNNNNTTYG 2 cut(s) 527, 559
AseI ATTAAT 1 cut(s) 737
AspLEI GCGC 1 cut(s) 932
AspS9I GGNCC 3 cut(s) 70, 399, 496
AsuHPI GGTGA 2 cut(s) 176, 961
AvaI CYCGRG 1 cut(s) 963
AvaII GGWCC 3 cut(s) 70, 399, 496
BaeGI GKGCMC 1 cut(s) 151
BaeI ACNNNNGTAYC 2 cut(s) 843, 876
BanI GGYRCC 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 519
BbvI GCAGC 4 cut(s) 288, 569, 606, 778
BcgI CGANNNNNNTGC 2 cut(s) 531, 565
BciT130I CCWGG 1 cut(s) 33
BcoDI GTCTC 2 cut(s) 142, 263
BfaI CTAG 2 cut(s) 281, 648
BfmI CTRYAG 2 cut(s) 58, 450
BglII AGATCT 1 cut(s) 16
BisI GCNGC 5 cut(s) 302, 558, 620, 750, 792
BlpI GCTNAGC 1 cut(s) 926
BlsI GCNGC 5 cut(s) 303, 559, 621, 751, 793
Bme1390I CCNGG 1 cut(s) 33
Bme18I GGWCC 3 cut(s) 70, 399, 496
BmeT110I CYCGRG 1 cut(s) 963
BmgT120I GGNCC 3 cut(s) 70, 399, 496
BmiI GGNNCC 5 cut(s) 71, 148, 401, 498, 985
BmrFI CCNGG 1 cut(s) 33
BmrI ACTGGG 2 cut(s) 380, 707
BmsI GCATC 2 cut(s) 674, 861
BmuI ACTGGG 2 cut(s) 380, 707
BoxI GACNNNNGTC 1 cut(s) 283
BpiI GAAGAC 1 cut(s) 519
BplI GAGNNNNNCTC 2 cut(s) 872, 904
Bpu1102I GCTNAGC 1 cut(s) 926
BpuEI CTTGAG 4 cut(s) 245, 506, 617, 830
Bsc4I CCNNNNNNNGG 3 cut(s) 89, 90, 698
Bse1I ACTGG 3 cut(s) 280, 375, 702
Bse3DI GCAATG 1 cut(s) 585
BseBI CCWGG 1 cut(s) 33
BseLI CCNNNNNNNGG 3 cut(s) 89, 90, 698
BseMI GCAATG 1 cut(s) 585
BseMII CTCAG 3 cut(s) 30, 213, 855
BseNI ACTGG 3 cut(s) 280, 375, 702
BseRI GAGGAG 3 cut(s) 794, 881, 976
BseSI GKGCMC 1 cut(s) 151
BseXI GCAGC 4 cut(s) 288, 569, 606, 778
BseYI CCCAGC 2 cut(s) 89, 756
BshNI GGYRCC 1 cut(s) 146
BsiHKCI CYCGRG 1 cut(s) 963
BslFI GGGAC 1 cut(s) 385
BslI CCNNNNNNNGG 3 cut(s) 89, 90, 698
BsmAI GTCTC 2 cut(s) 142, 263
BsmFI GGGAC 1 cut(s) 385
BsmI GAATGC 1 cut(s) 184
BsoBI CYCGRG 1 cut(s) 963
Bsp1286I GDGCHC 1 cut(s) 151
Bsp143I GATC 2 cut(s) 16, 35
Bsp1720I GCTNAGC 1 cut(s) 926
BspACI CCGC 2 cut(s) 750, 923
BspCNI CTCAG 3 cut(s) 31, 214, 856
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 5 cut(s) 71, 148, 401, 498, 985
BspMAI CTGCAG 1 cut(s) 454
BspPI GGATC 1 cut(s) 43
BspT107I GGYRCC 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 585
BsrI ACTGG 3 cut(s) 280, 375, 702
BssMI GATC 2 cut(s) 16, 35
Bst2UI CCWGG 1 cut(s) 33
Bst4CI ACNGT 3 cut(s) 325, 479, 823
Bst6I CTCTTC 1 cut(s) 108
BstDEI CTNAG 6 cut(s) 11, 39, 222, 761, 864, 926
BstEII GGTNACC 1 cut(s) 949
BstHHI GCGC 1 cut(s) 932
BstKTI GATC 2 cut(s) 19, 38
BstMAI GTCTC 2 cut(s) 142, 263
BstMBI GATC 2 cut(s) 16, 35
BstMWI GCNNNNNNNGC 3 cut(s) 95, 566, 575
BstNI CCWGG 1 cut(s) 33
BstPAI GACNNNNGTC 1 cut(s) 283
BstPI GGTNACC 1 cut(s) 949
BstSCI CCNGG 1 cut(s) 31
BstSFI CTRYAG 2 cut(s) 58, 450
BstSLI GKGCMC 1 cut(s) 151
BstV1I GCAGC 4 cut(s) 288, 569, 606, 778
BstV2I GAAGAC 1 cut(s) 519
BstX2I RGATCY 2 cut(s) 16, 35
BstYI RGATCY 2 cut(s) 16, 35
BtsIMutI CAGTG 1 cut(s) 368
CaiI CAGNNNCTG 1 cut(s) 863
CciI TCATGA 1 cut(s) 109
CfoI GCGC 1 cut(s) 932
Cfr13I GGNCC 3 cut(s) 70, 399, 496
Csp6I GTAC 1 cut(s) 711
CviAII CATG 2 cut(s) 110, 588
CviQI GTAC 1 cut(s) 711
DdeI CTNAG 6 cut(s) 11, 39, 222, 761, 864, 926
DpnI GATC 2 cut(s) 18, 37
DpnII GATC 2 cut(s) 16, 35
Eam1104I CTCTTC 1 cut(s) 108
EarI CTCTTC 1 cut(s) 108
Eco32I GATATC 1 cut(s) 457
Eco47I GGWCC 3 cut(s) 70, 399, 496
Eco57I CTGAAG 1 cut(s) 425
Eco88I CYCGRG 1 cut(s) 963
Eco91I GGTNACC 1 cut(s) 949
EcoO109I RGGNCCY 2 cut(s) 70, 399
EcoO65I GGTNACC 1 cut(s) 949
EcoRI GAATTC 1 cut(s) 651
EcoRII CCWGG 1 cut(s) 31
EcoRV GATATC 1 cut(s) 457
FaeI CATG 2 cut(s) 113, 591
FalI AAGNNNNNCTT 4 cut(s) 254, 286, 927, 959
FaqI GGGAC 1 cut(s) 385
FatI CATG 2 cut(s) 109, 587
FauNDI CATATG 1 cut(s) 721
Fnu4HI GCNGC 5 cut(s) 302, 558, 620, 750, 792
Fsp4HI GCNGC 5 cut(s) 302, 558, 620, 750, 792
FspBI CTAG 2 cut(s) 281, 648
GlaI GCGC 1 cut(s) 931
GluI GCNGC 5 cut(s) 302, 558, 620, 750, 792
GsaI CCCAGC 2 cut(s) 93, 760
HhaI GCGC 1 cut(s) 932
Hin1II CATG 2 cut(s) 113, 591
Hin6I GCGC 1 cut(s) 930
HinP1I GCGC 1 cut(s) 930
HinfI GANTC 9 cut(s) 218, 256, 284, 382, 409, 488, 779, 841, 887
HphI GGTGA 2 cut(s) 176, 961
Hpy166II GTNNAC 1 cut(s) 886
Hpy188I TCNGA 4 cut(s) 40, 223, 840, 865
Hpy188III TCNNGA 5 cut(s) 110, 262, 776, 965, 990
Hpy8I GTNNAC 1 cut(s) 886
Hpy99I CGWCG 1 cut(s) 333
HpyAV CCTTC 4 cut(s) 162, 655, 701, 962
HpyCH4III ACNGT 3 cut(s) 325, 479, 823
HpyCH4V TGCA 9 cut(s) 182, 194, 429, 437, 452, 511, 622, 817, 874
HpyF10VI GCNNNNNNNGC 3 cut(s) 95, 566, 575
HpyF3I CTNAG 6 cut(s) 11, 39, 222, 761, 864, 926
Hsp92II CATG 2 cut(s) 113, 591
HspAI GCGC 1 cut(s) 930
Kzo9I GATC 2 cut(s) 16, 35
LmnI GCTCC 1 cut(s) 989
Lsp1109I GCAGC 4 cut(s) 288, 569, 606, 778
LweI GCATC 2 cut(s) 674, 861
MaeI CTAG 2 cut(s) 281, 648
MaeIII GTNAC 2 cut(s) 764, 949
MalI GATC 2 cut(s) 18, 37
MboI GATC 2 cut(s) 16, 35
MboII GAAGA 9 cut(s) 58, 125, 136, 254, 418, 451, 509, 519, 935
MflI RGATCY 2 cut(s) 16, 35
MhlI GDGCHC 1 cut(s) 151
MluCI AATT 5 cut(s) 438, 651, 734, 738, 833
MlyI GAGTC 4 cut(s) 227, 265, 293, 881
MmeI TCCRAC 1 cut(s) 875
MseI TTAA 2 cut(s) 272, 737
MslI CAYNNNNRTG 1 cut(s) 670
MspA1I CMGCKG 1 cut(s) 560
MspR9I CCNGG 1 cut(s) 33
Mva1269I GAATGC 1 cut(s) 184
MvaI CCWGG 1 cut(s) 33
MwoI GCNNNNNNNGC 3 cut(s) 95, 566, 575
NdeI CATATG 1 cut(s) 721
NdeII GATC 2 cut(s) 16, 35
NlaIII CATG 2 cut(s) 113, 591
NlaIV GGNNCC 5 cut(s) 71, 148, 401, 498, 985
NmuCI GTSAC 2 cut(s) 764, 949
PagI TCATGA 1 cut(s) 109
PctI GAATGC 1 cut(s) 184
PfeI GAWTC 5 cut(s) 382, 409, 488, 779, 841
PkrI GCNGC 5 cut(s) 303, 559, 621, 751, 793
PleI GAGTC 4 cut(s) 226, 264, 292, 881
PpsI GAGTC 4 cut(s) 226, 264, 292, 881
PpuMI RGGWCCY 2 cut(s) 70, 399
PshAI GACNNNNGTC 1 cut(s) 283
PshBI ATTAAT 1 cut(s) 737
Psp5II RGGWCCY 2 cut(s) 70, 399
Psp6I CCWGG 1 cut(s) 31
PspEI GGTNACC 1 cut(s) 949
PspFI CCCAGC 2 cut(s) 89, 756
PspGI CCWGG 1 cut(s) 31
PspN4I GGNNCC 5 cut(s) 71, 148, 401, 498, 985
PspPI GGNCC 3 cut(s) 70, 399, 496
PspPPI RGGWCCY 2 cut(s) 70, 399
PstI CTGCAG 1 cut(s) 454
PstNI CAGNNNCTG 1 cut(s) 863
PsuI RGATCY 2 cut(s) 16, 35
PvuII CAGCTG 1 cut(s) 560
RsaI GTAC 1 cut(s) 712
RsaNI GTAC 1 cut(s) 711
RseI CAYNNNNRTG 1 cut(s) 670
SaqAI TTAA 2 cut(s) 272, 737
SatI GCNGC 5 cut(s) 302, 558, 620, 750, 792
Sau3AI GATC 2 cut(s) 16, 35
Sau96I GGNCC 3 cut(s) 70, 399, 496
SchI GAGTC 4 cut(s) 227, 265, 293, 881
ScrFI CCNGG 1 cut(s) 33
SduI GDGCHC 1 cut(s) 151
SfaNI GCATC 2 cut(s) 674, 861
SfcI CTRYAG 2 cut(s) 58, 450
SinI GGWCC 3 cut(s) 70, 399, 496
SmiMI CAYNNNNRTG 1 cut(s) 670
SmlI CTYRAG 4 cut(s) 260, 521, 632, 809
SmoI CTYRAG 4 cut(s) 260, 521, 632, 809
Sse9I AATT 5 cut(s) 438, 651, 734, 738, 833
SsiI CCGC 2 cut(s) 750, 923
SspMI CTAG 2 cut(s) 281, 648
StyD4I CCNGG 1 cut(s) 31
TaaI ACNGT 3 cut(s) 325, 479, 823
TaqI TCGA 5 cut(s) 363, 655, 682, 796, 911
TasI AATT 5 cut(s) 438, 651, 734, 738, 833
TauI GCSGC 1 cut(s) 752
TfiI GAWTC 5 cut(s) 382, 409, 488, 779, 841
Tru1I TTAA 2 cut(s) 272, 737
Tru9I TTAA 2 cut(s) 272, 737
TscAI CASTG 1 cut(s) 375
TseFI GTSAC 2 cut(s) 764, 949
TseI GCWGC 4 cut(s) 301, 557, 619, 791
Tsp45I GTSAC 2 cut(s) 764, 949
TspDTI ATGAA 3 cut(s) 66, 126, 480
TspGWI ACGGA 1 cut(s) 317
TspRI CASTG 1 cut(s) 375
VpaK11BI GGWCC 3 cut(s) 70, 399, 496
VspI ATTAAT 1 cut(s) 737
XapI RAATTY 2 cut(s) 651, 833
XspI CTAG 2 cut(s) 281, 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.