MD17G1036400.v1.1

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007833
Physical Location & Seq
Reverse (-)
2592431 .. 2593679
1249 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1036400.v1.1.491

Sequence Viewer

Length: 648 bp
ATGTGGAGGGTGTTACATCATGCCCTTGCTACCATGGATGTGCTTTTTATTCGTCACTGTTCCCCTTCACCAATTTGCCCTATTTGCCATGCTTATGATGAATCTGTGGAACATTTGTTCTTTTTGTGCCCGTGGGTTGAGCCCATTTGGTTTGGTGCTAATGCTGAAGCGGCTGAAGGTTCTGCGATTCTCATTGGTTGTGAAGTGGCTGTTTCGTTGGGGTTGGCTCAGGTAATTGTGGAATCTGATTCAAAAGAGAATATTTCAAATCTGGAGAATGTGGCTTCTTCTGGCAGTTGGGAGGCTTTTCCCATTCGGGGTGCGTTTGGTACGTGGGACGGGACGGGACGGAACGGGACGAGGCGTTCCGTCCCGCGTTTGGTGCGCCAAAAATGGGTGGAACGGGCTGTTCCACGGGACAGATTTTTGGTGTTTTTGCGTCCCACCTCCACCCCCTGGAACGGGTTTGTTCCACGTCTGTGGAACACAATGTTTTACCATTTTAAGACAAATATACCCCATGCCTTTTTCAAAAATTACACCTTCGTTCCGTCCCGTCCCGTCCCGTTCCGTCCCGTCCCGTCCCGTCCCGTCTGCGTACCAAACGCACCCGTATTGAACAAAGATGGGCTTCCTTGCCCCCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

216

Amino Acids

24.22

Weight (kDa)

8.77

Isoelectric Point (pI)

52.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 1 - 50 4.2e-09 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 456
AccII CGCG 1 cut(s) 376
AciI CCGC 2 cut(s) 170, 374
AcuI CTGAAG 2 cut(s) 186, 195
AfaI GTAC 2 cut(s) 331, 600
AfiI CCNNNNNNNGG 6 cut(s) 317, 379, 394, 456, 461, 462
AgsI TTSAA 4 cut(s) 252, 267, 532, 619
AjiI CACGTC 1 cut(s) 476
AjnI CCWGG 1 cut(s) 455
AleI CACNNNNGTG 1 cut(s) 478
AloI GAACNNNNNNTCC 4 cut(s) 101, 133, 349, 381
AspLEI GCGC 1 cut(s) 387
AsuHPI GGTGA 1 cut(s) 60
BaeGI GKGCMC 1 cut(s) 131
BanII GRGCYC 1 cut(s) 144
BccI CCATC 1 cut(s) 620
BciT130I CCWGG 1 cut(s) 457
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
Bme1390I CCNGG 1 cut(s) 457
BmgBI CACGTC 1 cut(s) 476
BmrFI CCNGG 1 cut(s) 457
BpmI CTGGAG 1 cut(s) 293
Bpu10I CCTNAGC 1 cut(s) 228
BsaAI YACGTR 1 cut(s) 333
BsaJI CCNNGG 4 cut(s) 33, 131, 413, 455
Bsc4I CCNNNNNNNGG 6 cut(s) 317, 379, 394, 456, 461, 462
BseBI CCWGG 1 cut(s) 457
BseDI CCNNGG 4 cut(s) 33, 131, 413, 455
BseGI GGATG 1 cut(s) 43
BseLI CCNNNNNNNGG 6 cut(s) 317, 379, 394, 456, 461, 462
BseMII CTCAG 1 cut(s) 242
BseSI GKGCMC 1 cut(s) 131
Bsh1236I CGCG 1 cut(s) 376
BslI CCNNNNNNNGG 6 cut(s) 317, 379, 394, 456, 461, 462
Bsp1286I GDGCHC 2 cut(s) 131, 144
Bsp19I CCATGG 1 cut(s) 33
BspACI CCGC 2 cut(s) 170, 374
BspCNI CTCAG 1 cut(s) 241
BspFNI CGCG 1 cut(s) 376
BssECI CCNNGG 4 cut(s) 33, 131, 413, 455
BssT1I CCWWGG 1 cut(s) 33
Bst2UI CCWGG 1 cut(s) 457
Bst4CI ACNGT 1 cut(s) 59
BstBAI YACGTR 1 cut(s) 333
BstDEI CTNAG 1 cut(s) 228
BstDSI CCRYGG 3 cut(s) 33, 131, 413
BstF5I GGATG 1 cut(s) 43
BstFNI CGCG 1 cut(s) 376
BstHHI GCGC 1 cut(s) 387
BstMWI GCNNNNNNNGC 3 cut(s) 84, 170, 382
BstNI CCWGG 1 cut(s) 457
BstSCI CCNGG 1 cut(s) 455
BstSLI GKGCMC 1 cut(s) 131
BstUI CGCG 1 cut(s) 376
BstXI CCANNNNNNTGG 1 cut(s) 480
BtgI CCRYGG 3 cut(s) 33, 131, 413
BtrI CACGTC 1 cut(s) 476
BtsCI GGATG 1 cut(s) 43
BtsIMutI CAGTG 1 cut(s) 55
CfoI GCGC 1 cut(s) 387
CseI GACGC 1 cut(s) 428
Csp6I GTAC 2 cut(s) 330, 599
CviAII CATG 4 cut(s) 20, 34, 89, 521
CviJI RGCY 8 cut(s) 142, 173, 209, 227, 284, 305, 407, 631
CviKI_1 RGCY 8 cut(s) 142, 173, 209, 227, 284, 305, 407, 631
CviQI GTAC 2 cut(s) 330, 599
DdeI CTNAG 1 cut(s) 228
Eco130I CCWWGG 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 144
Eco57I CTGAAG 2 cut(s) 186, 195
EcoRII CCWGG 1 cut(s) 455
EcoT14I CCWWGG 1 cut(s) 33
EcoT38I GRGCYC 1 cut(s) 144
ErhI CCWWGG 1 cut(s) 33
FaeI CATG 4 cut(s) 23, 37, 92, 524
FaiI YATR 6 cut(s) 21, 35, 90, 96, 515, 522
FalI AAGNNNNNCTT 2 cut(s) 615, 647
FatI CATG 4 cut(s) 19, 33, 88, 520
FauI CCCGC 1 cut(s) 381
Fnu4HI GCNGC 1 cut(s) 171
FokI GGATG 1 cut(s) 50
FriOI GRGCYC 1 cut(s) 144
Fsp4HI GCNGC 1 cut(s) 171
GlaI GCGC 1 cut(s) 386
GluI GCNGC 1 cut(s) 171
GsuI CTGGAG 1 cut(s) 293
HgaI GACGC 1 cut(s) 428
HhaI GCGC 1 cut(s) 387
Hin1II CATG 4 cut(s) 23, 37, 92, 524
Hin6I GCGC 1 cut(s) 385
HinP1I GCGC 1 cut(s) 385
HinfI GANTC 4 cut(s) 101, 187, 242, 248
HphI GGTGA 1 cut(s) 60
Hpy188I TCNGA 1 cut(s) 247
Hpy188III TCNNGA 1 cut(s) 272
HpyAV CCTTC 3 cut(s) 75, 170, 553
HpyCH4III ACNGT 1 cut(s) 59
HpyCH4IV ACGT 2 cut(s) 332, 475
HpyF10VI GCNNNNNNNGC 3 cut(s) 84, 170, 382
HpyF3I CTNAG 1 cut(s) 228
HpySE526I ACGT 2 cut(s) 332, 475
Hsp92II CATG 4 cut(s) 23, 37, 92, 524
HspAI GCGC 1 cut(s) 385
LpnPI CCDG 5 cut(s) 215, 257, 276, 442, 469
MaeII ACGT 2 cut(s) 332, 475
MaeIII GTNAC 2 cut(s) 12, 53
MboII GAAGA 1 cut(s) 279
MhlI GDGCHC 2 cut(s) 131, 144
MluCI AATT 3 cut(s) 72, 234, 535
MnlI CCTC 3 cut(s) 295, 354, 457
MseI TTAA 2 cut(s) 504, 646
MslI CAYNNNNRTG 3 cut(s) 38, 93, 478
MspR9I CCNGG 1 cut(s) 457
MvaI CCWGG 1 cut(s) 457
MvnI CGCG 1 cut(s) 376
MwoI GCNNNNNNNGC 3 cut(s) 84, 170, 382
NcoI CCATGG 1 cut(s) 33
NlaIII CATG 4 cut(s) 23, 37, 92, 524
NmuCI GTSAC 1 cut(s) 53
OliI CACNNNNGTG 1 cut(s) 478
PfeI GAWTC 4 cut(s) 101, 187, 242, 248
PflMI CCANNNNNTGG 1 cut(s) 456
PkrI GCNGC 1 cut(s) 172
Ppu21I YACGTR 1 cut(s) 333
Psp6I CCWGG 1 cut(s) 455
PspGI CCWGG 1 cut(s) 455
RsaI GTAC 2 cut(s) 331, 600
RsaNI GTAC 2 cut(s) 330, 599
RseI CAYNNNNRTG 3 cut(s) 38, 93, 478
SaqAI TTAA 2 cut(s) 504, 646
SatI GCNGC 1 cut(s) 171
ScrFI CCNGG 1 cut(s) 457
SduI GDGCHC 2 cut(s) 131, 144
SetI ASST 6 cut(s) 181, 234, 335, 449, 478, 545
SmiMI CAYNNNNRTG 3 cut(s) 38, 93, 478
Sse9I AATT 3 cut(s) 72, 234, 535
SsiI CCGC 2 cut(s) 170, 374
SspI AATATT 1 cut(s) 262
StyD4I CCNGG 1 cut(s) 455
StyI CCWWGG 1 cut(s) 33
TaaI ACNGT 1 cut(s) 59
TaiI ACGT 2 cut(s) 335, 478
TasI AATT 3 cut(s) 72, 234, 535
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 4 cut(s) 101, 187, 242, 248
Tru1I TTAA 2 cut(s) 504, 646
Tru9I TTAA 2 cut(s) 504, 646
TscAI CASTG 1 cut(s) 62
TseFI GTSAC 1 cut(s) 53
Tsp45I GTSAC 1 cut(s) 53
TspDTI ATGAA 1 cut(s) 114
TspGWI ACGGA 4 cut(s) 358, 364, 540, 560
TspRI CASTG 1 cut(s) 62
Van91I CCANNNNNTGG 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.