pycom07g15430

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007833
Physical Location & Seq
Forward (+)
17745914 .. 17748240
2327 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g15430.2

Sequence Viewer

Length: 744 bp
ATGTGGGCAGTGTCCTACCCTCCCCCACCACCACCATGCCAACCTCTCATCCCCAAACCACCTCCGATGAGCCCCGTCCGCCTCTCTGTAGCCGCCAAGTCTCAGCCTCGATCGCAACACCTCGGAGGCTTAGGCCGATCGGTCATTACAAAACTCAAATCCAATCCCGACCCAAAATTACAGGGAAACCAAAAGCAAGATGAAGAGGGTAAGAAGGTAATTAGCGGGTCGGATGTGCTTTTCGCCATGCAGAGAGCTGCGGCCAAGAAAAACAGACAGAGCGGTAAGAAAAAGAATAAGGGAAAGGTGTCGTCGTCGTTATCGTCACCATCCTATGCGGGCAGTCACAGGGAAGAGGAGGGTGTGGACGAGTACGGTAGCGTACCGCTTGGTACGTGGGACGGGACGGAACAGAGTGGGACAAGGCGTTCCGTCCCACGTTTGGTGCTCTTAAAACGGGTGGAACGAGCTGTTCCACGGGACGAGTTTTGGGTGAATTTTTGTTCCACCTCAACCCCCTGGAACGACTCGTTCCACATTCGTGGAACACAAAATTATAACCTCTTCGTCTCCTTCTTCTTCCTCCTTGTTTCCATCCGAGGGCATCTTTGCTCCCGCTCCGTTCCGTTCCGTCCCGTCCAGTCCCGTTCCGTCCCGTCCAGTCACATTCTGTTCCGTCCCATTCCATTTCATTCCGTCTCGTCCCGTCTGCATACCAAACGATACCATAATGAATTACCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

248

Amino Acids

27.61

Weight (kDa)

10.39

Isoelectric Point (pI)

66.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 558
AccBSI CCGCTC 2 cut(s) 282, 618
AciI CCGC 8 cut(s) 79, 93, 225, 260, 282, 338, 386, 616
AcoI YGGCCR 1 cut(s) 261
AcsI RAATTY 1 cut(s) 496
AfaI GTAC 3 cut(s) 374, 384, 394
AfiI CCNNNNNNNGG 2 cut(s) 442, 600
AjnI CCWGG 1 cut(s) 518
AleI CACNNNNGTG 1 cut(s) 540
AloI GAACNNNNNNTCC 2 cut(s) 412, 444
AluBI AGCT 2 cut(s) 257, 470
AluI AGCT 2 cut(s) 257, 470
Alw21I GWGCWC 1 cut(s) 450
Alw26I GTCTC 3 cut(s) 105, 574, 703
AoxI GGCC 2 cut(s) 133, 261
ApeKI GCWGC 1 cut(s) 257
ApoI RAATTY 1 cut(s) 496
AsuHPI GGTGA 2 cut(s) 318, 505
BanII GRGCYC 1 cut(s) 74
Bbv12I GWGCWC 1 cut(s) 450
BbvI GCAGC 1 cut(s) 244
BccI CCATC 2 cut(s) 337, 602
BciT130I CCWGG 1 cut(s) 520
BcoDI GTCTC 3 cut(s) 105, 574, 703
BfmI CTRYAG 1 cut(s) 87
BisI GCNGC 3 cut(s) 93, 258, 261
BlsI GCNGC 3 cut(s) 94, 259, 262
Bme1390I CCNGG 1 cut(s) 520
BmrFI CCNGG 1 cut(s) 520
BmsI GCATC 1 cut(s) 613
Bpu10I CCTNAGC 1 cut(s) 130
BsaAI YACGTR 1 cut(s) 396
BsaJI CCNNGG 4 cut(s) 121, 476, 518, 598
Bsc4I CCNNNNNNNGG 2 cut(s) 442, 600
Bse1I ACTGG 2 cut(s) 640, 660
BseBI CCWGG 1 cut(s) 520
BseDI CCNNGG 4 cut(s) 121, 476, 518, 598
BseGI GGATG 4 cut(s) 48, 238, 329, 594
BseLI CCNNNNNNNGG 2 cut(s) 442, 600
BseMII CTCAG 1 cut(s) 116
BseNI ACTGG 2 cut(s) 640, 660
BseRI GAGGAG 1 cut(s) 371
BseXI GCAGC 1 cut(s) 244
Bsh1285I CGRYCG 2 cut(s) 113, 140
BshFI GGCC 2 cut(s) 135, 263
BsiEI CGRYCG 2 cut(s) 113, 140
BsiHKAI GWGCWC 1 cut(s) 450
BslI CCNNNNNNNGG 2 cut(s) 442, 600
BsmAI GTCTC 3 cut(s) 105, 574, 703
BsmBI CGTCTC 2 cut(s) 574, 703
BsnI GGCC 2 cut(s) 135, 263
Bsp1286I GDGCHC 2 cut(s) 74, 450
Bsp143I GATC 2 cut(s) 110, 137
BspACI CCGC 8 cut(s) 79, 93, 225, 260, 282, 338, 386, 616
BspANI GGCC 2 cut(s) 135, 263
BspCNI CTCAG 1 cut(s) 115
BsrBI CCGCTC 2 cut(s) 282, 618
BsrI ACTGG 2 cut(s) 640, 660
BssECI CCNNGG 4 cut(s) 121, 476, 518, 598
BssMI GATC 2 cut(s) 110, 137
Bst2UI CCWGG 1 cut(s) 520
Bst4CI ACNGT 2 cut(s) 377, 741
Bst6I CTCTTC 3 cut(s) 198, 348, 569
BstBAI YACGTR 1 cut(s) 396
BstC8I GCNNGC 1 cut(s) 340
BstDEI CTNAG 2 cut(s) 102, 130
BstDSI CCRYGG 1 cut(s) 476
BstF5I GGATG 4 cut(s) 48, 238, 329, 594
BstKTI GATC 2 cut(s) 113, 140
BstMAI GTCTC 3 cut(s) 105, 574, 703
BstMBI GATC 2 cut(s) 110, 137
BstMCI CGRYCG 2 cut(s) 113, 140
BstMWI GCNNNNNNNGC 2 cut(s) 78, 112
BstNI CCWGG 1 cut(s) 520
BstSCI CCNGG 1 cut(s) 518
BstSFI CTRYAG 1 cut(s) 87
BstV1I GCAGC 1 cut(s) 244
BstXI CCANNNNNNTGG 1 cut(s) 542
BsuRI GGCC 2 cut(s) 135, 263
BtgI CCRYGG 1 cut(s) 476
BtsCI GGATG 4 cut(s) 48, 238, 329, 594
BtsI GCAGTG 1 cut(s) 15
BtsIMutI CAGTG 1 cut(s) 15
Cac8I GCNNGC 1 cut(s) 340
Csp6I GTAC 3 cut(s) 373, 383, 393
CviAII CATG 2 cut(s) 36, 247
CviJI RGCY 8 cut(s) 72, 92, 106, 129, 135, 257, 263, 470
CviKI_1 RGCY 8 cut(s) 72, 92, 106, 129, 135, 257, 263, 470
CviQI GTAC 3 cut(s) 373, 383, 393
DdeI CTNAG 2 cut(s) 102, 130
DpnI GATC 2 cut(s) 112, 139
DpnII GATC 2 cut(s) 110, 137
EaeI YGGCCR 1 cut(s) 261
Eam1104I CTCTTC 3 cut(s) 198, 348, 569
EarI CTCTTC 3 cut(s) 198, 348, 569
EciI GGCGGA 1 cut(s) 68
Eco24I GRGCYC 1 cut(s) 74
EcoRII CCWGG 1 cut(s) 518
EcoT38I GRGCYC 1 cut(s) 74
Esp3I CGTCTC 2 cut(s) 574, 703
FaeI CATG 2 cut(s) 39, 250
FaiI YATR 6 cut(s) 37, 248, 336, 558, 714, 729
FatI CATG 2 cut(s) 35, 246
FauI CCCGC 3 cut(s) 218, 331, 623
Fnu4HI GCNGC 3 cut(s) 93, 258, 261
FokI GGATG 4 cut(s) 35, 245, 316, 581
FriOI GRGCYC 1 cut(s) 74
Fsp4HI GCNGC 3 cut(s) 93, 258, 261
GluI GCNGC 3 cut(s) 93, 258, 261
HaeIII GGCC 2 cut(s) 135, 263
Hin1II CATG 2 cut(s) 39, 250
HinfI GANTC 1 cut(s) 527
HphI GGTGA 2 cut(s) 318, 505
Hpy166II GTNNAC 1 cut(s) 367
Hpy188I TCNGA 4 cut(s) 66, 125, 232, 599
Hpy188III TCNNGA 1 cut(s) 167
Hpy8I GTNNAC 1 cut(s) 367
Hpy99I CGWCG 2 cut(s) 316, 319
HpyAV CCTTC 2 cut(s) 208, 583
HpyCH4III ACNGT 2 cut(s) 377, 741
HpyCH4IV ACGT 2 cut(s) 395, 439
HpyCH4V TGCA 2 cut(s) 250, 712
HpyF10VI GCNNNNNNNGC 2 cut(s) 78, 112
HpyF3I CTNAG 2 cut(s) 102, 130
HpySE526I ACGT 2 cut(s) 395, 439
Hsp92II CATG 2 cut(s) 39, 250
Kzo9I GATC 2 cut(s) 110, 137
LmnI GCTCC 2 cut(s) 617, 623
LpnPI CCDG 6 cut(s) 167, 334, 505, 532, 653, 673
Lsp1109I GCAGC 1 cut(s) 244
LweI GCATC 1 cut(s) 613
MaeII ACGT 2 cut(s) 395, 439
MaeIII GTNAC 3 cut(s) 324, 344, 662
MalI GATC 2 cut(s) 112, 139
MbiI CCGCTC 2 cut(s) 282, 618
MboI GATC 2 cut(s) 110, 137
MboII GAAGA 5 cut(s) 215, 365, 556, 568, 571
MhlI GDGCHC 2 cut(s) 74, 450
MluCI AATT 5 cut(s) 176, 219, 496, 553, 734
MlyI GAGTC 1 cut(s) 521
MmeI TCCRAC 1 cut(s) 210
MseI TTAA 1 cut(s) 452
MslI CAYNNNNRTG 2 cut(s) 34, 540
MspR9I CCNGG 1 cut(s) 520
MvaI CCWGG 1 cut(s) 520
MwoI GCNNNNNNNGC 2 cut(s) 78, 112
NdeII GATC 2 cut(s) 110, 137
NlaIII CATG 2 cut(s) 39, 250
NmuCI GTSAC 3 cut(s) 324, 344, 662
OliI CACNNNNGTG 1 cut(s) 540
PcsI WCGNNNNNNNCGW 2 cut(s) 320, 463
PkrI GCNGC 3 cut(s) 94, 259, 262
Ple19I CGATCG 2 cut(s) 113, 140
PleI GAGTC 1 cut(s) 521
PpsI GAGTC 1 cut(s) 521
Ppu21I YACGTR 1 cut(s) 396
PsiI TTATAA 1 cut(s) 558
Psp6I CCWGG 1 cut(s) 518
PspGI CCWGG 1 cut(s) 518
PvuI CGATCG 2 cut(s) 113, 140
RsaI GTAC 3 cut(s) 374, 384, 394
RsaNI GTAC 3 cut(s) 373, 383, 393
RseI CAYNNNNRTG 2 cut(s) 34, 540
SaqAI TTAA 1 cut(s) 452
SatI GCNGC 3 cut(s) 93, 258, 261
Sau3AI GATC 2 cut(s) 110, 137
SchI GAGTC 1 cut(s) 521
ScrFI CCNGG 1 cut(s) 520
SduI GDGCHC 2 cut(s) 74, 450
SfaNI GCATC 1 cut(s) 613
SfcI CTRYAG 1 cut(s) 87
SmiMI CAYNNNNRTG 2 cut(s) 34, 540
Sse9I AATT 5 cut(s) 176, 219, 496, 553, 734
SsiI CCGC 8 cut(s) 79, 93, 225, 260, 282, 338, 386, 616
StyD4I CCNGG 1 cut(s) 518
TaaI ACNGT 2 cut(s) 377, 741
TaiI ACGT 2 cut(s) 398, 442
TaqI TCGA 1 cut(s) 109
TaqII GACCGA 1 cut(s) 130
TasI AATT 5 cut(s) 176, 219, 496, 553, 734
TauI GCSGC 2 cut(s) 95, 263
Tru1I TTAA 1 cut(s) 452
Tru9I TTAA 1 cut(s) 452
TscAI CASTG 1 cut(s) 15
TseFI GTSAC 3 cut(s) 324, 344, 662
TseI GCWGC 1 cut(s) 257
Tsp45I GTSAC 3 cut(s) 324, 344, 662
TspDTI ATGAA 2 cut(s) 216, 680
TspGWI ACGGA 8 cut(s) 421, 422, 610, 615, 620, 640, 665, 685
TspRI CASTG 1 cut(s) 15
XapI RAATTY 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.