pycom14g16070

Ent-kaurenoic acid oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0007833
Physical Location & Seq
Forward (+)
18499898 .. 18500452
555 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g16070.1

Sequence Viewer

Length: 555 bp
ATGAGCCTTGGTATGTGCTTCCTCTCAGACTATGACATTCGCCTACCGGCGCTAAATTGCCGCCTGGCCACATGCATGGGACTTCCATTTTTGGGTGAAATATTCACCTTCCTTTGGTACTTCAAAATTGTTCGTCGTCCTGACGACTTTATCAATGCTAAGCGGGCTAAGTACGATTCCATTTCTATTTTGTTCTATATGGTACCGTTTGGTACGTGGGATGGGACGGAACAGAGTGGGACAAGACGTTCCGTCCCACGTTTGGTGCGCCTAAAACGGGTGGAACGAGCTGTTCCACGGGACGAGTTTTGGGTGAATTTTCGTTCCACCTCACCCCCTGGAACGACTCGTTCCACATCCGTGGAACACAAAATTATAACCTCTCCGTCTCCTTCTTCTTCCTCCTTGTTTCCATCCGAGGGCATCTTTGCTCCCGTTCCGTCCTGTCCCGTCCCATTCCGTTCCGTTCCGTCCCGTCTGCATACCAAACGATACCTATATGCATTTATGCTAACTAGTAATATTAACATTTGGAAAAGAAAAAAAAAATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00904 ko01100 ko01110 map00904 map01100 map01110
Pfam Domains
Protein Families
p450

Protein Analysis

185

Amino Acids

21.29

Weight (kDa)

10.21

Isoelectric Point (pI)

89.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 377
Acc65I GGTACC 1 cut(s) 202
AccB1I GGYRCC 1 cut(s) 202
AciI CCGC 2 cut(s) 61, 163
AcoI YGGCCR 1 cut(s) 66
AcsI RAATTY 1 cut(s) 316
AfaI GTAC 4 cut(s) 119, 173, 204, 214
AfiI CCNNNNNNNGG 5 cut(s) 92, 114, 262, 277, 419
AgsI TTSAA 1 cut(s) 124
AhdI GACNNNNNGTC 1 cut(s) 251
AhlI ACTAGT 1 cut(s) 515
AjnI CCWGG 2 cut(s) 63, 337
AleI CACNNNNGTG 1 cut(s) 359
AloI GAACNNNNNNTCC 2 cut(s) 232, 264
AluBI AGCT 1 cut(s) 290
AluI AGCT 1 cut(s) 290
Alw26I GTCTC 1 cut(s) 393
AoxI GGCC 1 cut(s) 66
ApoI RAATTY 1 cut(s) 316
Asp718I GGTACC 1 cut(s) 202
AspLEI GCGC 2 cut(s) 52, 270
AsuHPI GGTGA 4 cut(s) 97, 107, 324, 325
BalI TGGCCA 1 cut(s) 68
BanI GGYRCC 1 cut(s) 202
BccI CCATC 2 cut(s) 215, 421
BciT130I CCWGG 2 cut(s) 65, 339
BcoDI GTCTC 1 cut(s) 393
BcuI ACTAGT 1 cut(s) 515
BfaI CTAG 1 cut(s) 516
BfoI RGCGCY 1 cut(s) 53
BisI GCNGC 1 cut(s) 61
BlpI GCTNAGC 1 cut(s) 159
BlsI GCNGC 1 cut(s) 62
Bme1390I CCNGG 2 cut(s) 65, 339
BmeRI GACNNNNNGTC 1 cut(s) 251
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 2 cut(s) 65, 339
BmsI GCATC 1 cut(s) 432
Bpu1102I GCTNAGC 1 cut(s) 159
BsaAI YACGTR 1 cut(s) 216
BsaJI CCNNGG 5 cut(s) 7, 296, 337, 360, 417
Bsc4I CCNNNNNNNGG 5 cut(s) 92, 114, 262, 277, 419
Bse118I RCCGGY 1 cut(s) 46
BseBI CCWGG 2 cut(s) 65, 339
BseDI CCNNGG 5 cut(s) 7, 296, 337, 360, 417
BseGI GGATG 3 cut(s) 226, 356, 413
BseLI CCNNNNNNNGG 5 cut(s) 92, 114, 262, 277, 419
BseMII CTCAG 1 cut(s) 39
BshFI GGCC 1 cut(s) 68
BshNI GGYRCC 1 cut(s) 202
BsiSI CCGG 1 cut(s) 47
BslFI GGGAC 8 cut(s) 93, 238, 239, 253, 314, 432, 437, 457
BslI CCNNNNNNNGG 5 cut(s) 92, 114, 262, 277, 419
BsmAI GTCTC 1 cut(s) 393
BsmBI CGTCTC 1 cut(s) 393
BsmFI GGGAC 8 cut(s) 93, 238, 239, 253, 314, 432, 437, 457
BsnI GGCC 1 cut(s) 68
Bsp1720I GCTNAGC 1 cut(s) 159
BspACI CCGC 2 cut(s) 61, 163
BspANI GGCC 1 cut(s) 68
BspCNI CTCAG 1 cut(s) 38
BspLI GGNNCC 1 cut(s) 204
BspT107I GGYRCC 1 cut(s) 202
BsrFI RCCGGY 1 cut(s) 46
BssAI RCCGGY 1 cut(s) 46
BssECI CCNNGG 5 cut(s) 7, 296, 337, 360, 417
BssT1I CCWWGG 1 cut(s) 7
Bst2UI CCWGG 2 cut(s) 65, 339
Bst4CI ACNGT 1 cut(s) 207
BstBAI YACGTR 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 165
BstDEI CTNAG 3 cut(s) 25, 159, 168
BstDSI CCRYGG 2 cut(s) 296, 360
BstF5I GGATG 3 cut(s) 226, 356, 413
BstH2I RGCGCY 1 cut(s) 53
BstHHI GCGC 2 cut(s) 52, 270
BstMAI GTCTC 1 cut(s) 393
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstNI CCWGG 2 cut(s) 65, 339
BstNSI RCATGY 1 cut(s) 75
BstSCI CCNGG 2 cut(s) 63, 337
BstXI CCANNNNNNTGG 2 cut(s) 76, 361
BsuRI GGCC 1 cut(s) 68
BtgI CCRYGG 2 cut(s) 296, 360
BtsCI GGATG 3 cut(s) 226, 356, 413
Cac8I GCNNGC 1 cut(s) 165
CfoI GCGC 2 cut(s) 52, 270
Cfr10I RCCGGY 1 cut(s) 46
Csp6I GTAC 4 cut(s) 118, 172, 203, 213
CviAII CATG 2 cut(s) 72, 76
CviJI RGCY 4 cut(s) 6, 68, 167, 290
CviKI_1 RGCY 4 cut(s) 6, 68, 167, 290
CviQI GTAC 4 cut(s) 118, 172, 203, 213
DdeI CTNAG 3 cut(s) 25, 159, 168
DriI GACNNNNNGTC 1 cut(s) 251
EaeI YGGCCR 1 cut(s) 66
Eam1105I GACNNNNNGTC 1 cut(s) 251
Eco130I CCWWGG 1 cut(s) 7
EcoRII CCWGG 2 cut(s) 63, 337
EcoT14I CCWWGG 1 cut(s) 7
EcoT22I ATGCAT 2 cut(s) 77, 505
ErhI CCWWGG 1 cut(s) 7
Esp3I CGTCTC 1 cut(s) 393
FaeI CATG 2 cut(s) 75, 79
FaqI GGGAC 8 cut(s) 93, 238, 239, 253, 314, 432, 437, 457
FatI CATG 2 cut(s) 71, 75
FauI CCCGC 1 cut(s) 156
Fnu4HI GCNGC 1 cut(s) 61
FokI GGATG 3 cut(s) 233, 343, 400
Fsp4HI GCNGC 1 cut(s) 61
FspBI CTAG 1 cut(s) 516
GlaI GCGC 2 cut(s) 51, 269
GluI GCNGC 1 cut(s) 61
HaeII RGCGCY 1 cut(s) 53
HaeIII GGCC 1 cut(s) 68
HapII CCGG 1 cut(s) 47
HhaI GCGC 2 cut(s) 52, 270
Hin1II CATG 2 cut(s) 75, 79
Hin6I GCGC 2 cut(s) 50, 268
HinP1I GCGC 2 cut(s) 50, 268
HinfI GANTC 2 cut(s) 176, 346
HpaII CCGG 1 cut(s) 47
HphI GGTGA 4 cut(s) 97, 107, 324, 325
Hpy188I TCNGA 2 cut(s) 28, 418
Hpy188III TCNNGA 1 cut(s) 140
Hpy99I CGWCG 1 cut(s) 138
HpyAV CCTTC 2 cut(s) 118, 402
HpyCH4III ACNGT 1 cut(s) 207
HpyCH4IV ACGT 3 cut(s) 215, 247, 259
HpyCH4V TGCA 3 cut(s) 75, 481, 503
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
HpyF3I CTNAG 3 cut(s) 25, 159, 168
HpySE526I ACGT 3 cut(s) 215, 247, 259
Hsp92II CATG 2 cut(s) 75, 79
HspAI GCGC 2 cut(s) 50, 268
KpnI GGTACC 1 cut(s) 206
LmnI GCTCC 1 cut(s) 436
LpnPI CCDG 7 cut(s) 50, 60, 77, 153, 324, 351, 457
LweI GCATC 1 cut(s) 432
MaeI CTAG 1 cut(s) 516
MaeII ACGT 3 cut(s) 215, 247, 259
MboII GAAGA 2 cut(s) 387, 390
MlsI TGGCCA 1 cut(s) 68
MluCI AATT 4 cut(s) 55, 126, 316, 372
MluNI TGGCCA 1 cut(s) 68
MlyI GAGTC 1 cut(s) 340
MnlI CCTC 5 cut(s) 32, 340, 391, 412, 412
Mox20I TGGCCA 1 cut(s) 68
Mph1103I ATGCAT 2 cut(s) 77, 505
MscI TGGCCA 1 cut(s) 68
MseI TTAA 1 cut(s) 525
MslI CAYNNNNRTG 2 cut(s) 74, 359
Msp20I TGGCCA 1 cut(s) 68
MspI CCGG 1 cut(s) 47
MspR9I CCNGG 2 cut(s) 65, 339
MvaI CCWGG 2 cut(s) 65, 339
MwoI GCNNNNNNNGC 1 cut(s) 164
NlaIII CATG 2 cut(s) 75, 79
NlaIV GGNNCC 1 cut(s) 204
NsiI ATGCAT 2 cut(s) 77, 505
NspI RCATGY 1 cut(s) 75
OliI CACNNNNGTG 1 cut(s) 359
PcsI WCGNNNNNNNCGW 1 cut(s) 283
PfeI GAWTC 1 cut(s) 176
PkrI GCNGC 1 cut(s) 62
PleI GAGTC 1 cut(s) 340
PpsI GAGTC 1 cut(s) 340
Ppu21I YACGTR 1 cut(s) 216
PsiI TTATAA 1 cut(s) 377
Psp6I CCWGG 2 cut(s) 63, 337
PspGI CCWGG 2 cut(s) 63, 337
PspN4I GGNNCC 1 cut(s) 204
RsaI GTAC 4 cut(s) 119, 173, 204, 214
RsaNI GTAC 4 cut(s) 118, 172, 203, 213
RseI CAYNNNNRTG 2 cut(s) 74, 359
SaqAI TTAA 1 cut(s) 525
SatI GCNGC 1 cut(s) 61
SchI GAGTC 1 cut(s) 340
ScrFI CCNGG 2 cut(s) 65, 339
SetI ASST 8 cut(s) 110, 218, 250, 262, 292, 332, 383, 498
SfaNI GCATC 1 cut(s) 432
SmiMI CAYNNNNRTG 2 cut(s) 74, 359
SpeI ACTAGT 1 cut(s) 515
Sse9I AATT 4 cut(s) 55, 126, 316, 372
SsiI CCGC 2 cut(s) 61, 163
SspI AATATT 2 cut(s) 102, 523
SspMI CTAG 1 cut(s) 516
StyD4I CCNGG 2 cut(s) 63, 337
StyI CCWWGG 1 cut(s) 7
TaaI ACNGT 1 cut(s) 207
TaiI ACGT 3 cut(s) 218, 250, 262
TasI AATT 4 cut(s) 55, 126, 316, 372
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 1 cut(s) 176
Tru1I TTAA 1 cut(s) 525
Tru9I TTAA 1 cut(s) 525
TspGWI ACGGA 8 cut(s) 241, 242, 349, 375, 429, 449, 454, 459
XapI RAATTY 1 cut(s) 316
XceI RCATGY 1 cut(s) 75
XspI CTAG 1 cut(s) 516
Zsp2I ATGCAT 2 cut(s) 77, 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.