Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0004428

Physical Location & Seq

Genomic Coordinates Reverse (-)

8656942 .. 8658500

Gene Structure 1559 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1102000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 888 bp

ATGGAAGCAGTCCAAGCTACAGAAGAAGAAATAATCACAACCCTACGTCTGGACGATGTCCAACCAACCTTGCCATCTGTTGATAAGTCAACGTCGTTGACTGATCACGAGCATCATCTAAATCTTGAAGAAGTGGAGAAGATTATAGGGTACAAATTCGAGAACAAGGTTTTGCTGCAGGAGGCTTTCACACATTCTTCCTTCGTCGCCGCCGAGTGGTCTGGCTTGTCCTACGAGCGTCTGGAGTATGTCGGGGACGCGGTGCTAAATTTGATGTTCAGCAGAGAACAGTACCTTTTGTACCCCGACTTGGCACCTGGGCCGTTGACACGGCTCCGGGCAGCCAATGTGGATACGGAGAAGCTTGCGCGTGTGGCGATCAAGCATGGGTTGTACCGGTACCTGCGGCATAGGAAACCCTTGCTTGAAGAACAAATTCGAGAATTTATGCGAGCAATGTCTGACTATCCACTGCATTCCAATGGTCTCATTGATGCTCCAAAGAACCTTGCGGACATTGTTGAGTCCACAATCGGTGCAGTTTTCATGGACTGCAAGTCAATTGACGTTGTGTGGAAGGTGTTCAAGAATTTGTTGAAGCCTATAATAGATCCGAAGACGATAAAGATGCATCCGGTGTCACAACTTCATGAAGTGTGTCAAAAAAACAATCTGAAACTTCGATTTGTCGATCTGTGGGAGGAGAAACACACGGTTGAGGTTTTTATAAACGATCAAATTGTTGGAAGAGGCACTTACAGTCTTAAGAAGGAAGTTGCACAAAATAGAGCAGCAAAAGATGCATTTGACAACATGTGGAATGTAATAGGTCAGGAGAAAGACAAAGTTCAAGACCATCAAGTAGACAATGGTCGATTGGAGGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ribonucleas_3_3 Ribonuclease_3 dsrm

Protein Analysis

296

Amino Acids

34.01

Weight (kDa)

5.61

Isoelectric Point (pI)

25.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ribonucleas_3_3	PF14622	55 - 196	9.2e-16	Ribonuclease-III-like
Ribonuclease_3	PF00636	79 - 185	6.6e-22	Ribonuclease III domain
dsrm	PF00035	212 - 271	2.2e-08	Double-stranded RNA binding motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004428)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15417 AT4G15417
fragaria_vesca	FvH4_5g08550 FvH4_6g42650
malus_domestica	MD09G1111700.v1.1 MD17G1102000.v1.1
prunus_persica	Prupe.3G213200_v2.0.a1
pyrus_communis	pycom09g03630 pycom17g09640
rosa_chinensis	RchiOBHm_Chr2g0158531 RchiOBHm_Chr7g0189841
rosa_laevigata	RLG00000004582 RLG00000021094
rosa_multiflora	Rmu_co8278635.1_g000001 Rmu_sc0000512.1_g000018 Rmu_sc0005896.1_g000003 Rmu_sc0005896.1_g000004 Rmu_sc0017496.1_g000006
rosa_roxburghii	Rroxscaffold_2G00091270 Rroxscaffold_3G00265090
rosa_rugosa	Rorug02G0469800 Rorug06G0500300
rosa_samantha	Rh2AG535800 Rh2BG549000 Rh2CG519500 Rh2DG558700 Rh7AG105600 Rh7BG108400 Rh7CG110400 Rh7DG108700
rosa_wichuraiana	Rw2G044370 Rw7G009150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	728
Acc36I	ACCTGC	1 cut(s)	411
Acc65I	GGTACC	1 cut(s)	399
AccB1I	GGYRCC	2 cut(s)	313, 399
AccI	GTMKAC	1 cut(s)	864
AccII	CGCG	2 cut(s)	260, 370
AciI	CCGC	4 cut(s)	210, 260, 406, 512
AclWI	GGATC	1 cut(s)	605
AcsI	RAATTY	5 cut(s)	155, 268, 435, 443, 589
AfaI	GTAC	5 cut(s)	152, 293, 302, 395, 401
AfiI	CCNNNNNNNGG	3 cut(s)	49, 216, 310
AflII	CTTAAG	1 cut(s)	764
AflIII	ACRYGT	1 cut(s)	813
AgeI	ACCGGT	1 cut(s)	396
AgsI	TTSAA	5 cut(s)	128, 428, 586, 598, 851
AhdI	GACNNNNNGTC	1 cut(s)	556
AjnI	CCWGG	1 cut(s)	316
AluBI	AGCT	2 cut(s)	17, 364
AluI	AGCT	2 cut(s)	17, 364
Alw26I	GTCTC	1 cut(s)	491
AlwI	GGATC	1 cut(s)	605
AoxI	GGCC	1 cut(s)	320
ApeKI	GCWGC	3 cut(s)	175, 341, 791
ApoI	RAATTY	5 cut(s)	155, 268, 435, 443, 589
AsiGI	ACCGGT	1 cut(s)	396
Asp700I	GAANNNNTTC	2 cut(s)	435, 581
Asp718I	GGTACC	1 cut(s)	399
AspLEI	GCGC	1 cut(s)	370
AspS9I	GGNCC	1 cut(s)	320
AsuC2I	CCSGG	1 cut(s)	338
BanI	GGYRCC	2 cut(s)	313, 399
BauI	CACGAG	1 cut(s)	107
BbsI	GAAGAC	1 cut(s)	623
BbvI	GCAGC	3 cut(s)	162, 353, 803
BccI	CCATC	2 cut(s)	82, 864
BceAI	ACGGC	2 cut(s)	307, 347
BcgI	CGANNNNNNTGC	2 cut(s)	610, 644
BciT130I	CCWGG	1 cut(s)	318
BciVI	GTATCC	1 cut(s)	346
BclI	TGATCA	1 cut(s)	103
BcnI	CCSGG	1 cut(s)	338
BcoDI	GTCTC	1 cut(s)	491
BfmI	CTRYAG	2 cut(s)	18, 176
BfrI	CTTAAG	1 cut(s)	764
BfuAI	ACCTGC	1 cut(s)	411
BfuI	GTATCC	1 cut(s)	346
BisI	GCNGC	5 cut(s)	176, 210, 342, 407, 792
BlsI	GCNGC	5 cut(s)	177, 211, 343, 408, 793
Bme1390I	CCNGG	2 cut(s)	318, 338
BmeRI	GACNNNNNGTC	1 cut(s)	556
BmgT120I	GGNCC	1 cut(s)	320
BmiI	GGNNCC	3 cut(s)	315, 335, 401
BmrFI	CCNGG	2 cut(s)	318, 338
BmsI	GCATC	5 cut(s)	121, 484, 618, 640, 790
BoxI	GACNNNNGTC	1 cut(s)	870
BpiI	GAAGAC	1 cut(s)	623
BpmI	CTGGAG	1 cut(s)	263
BpuMI	CCSGG	1 cut(s)	338
BsaI	GGTCTC	1 cut(s)	491
BsaJI	CCNNGG	1 cut(s)	317
BsaWI	WCCGGW	2 cut(s)	396, 634
Bsc4I	CCNNNNNNNGG	3 cut(s)	49, 216, 310
Bse118I	RCCGGY	1 cut(s)	396
Bse3DI	GCAATG	1 cut(s)	462
BseBI	CCWGG	1 cut(s)	318
BseDI	CCNNGG	1 cut(s)	317
BseGI	GGATG	1 cut(s)	631
BseLI	CCNNNNNNNGG	3 cut(s)	49, 216, 310
BseMI	GCAATG	1 cut(s)	462
BseRI	GAGGAG	1 cut(s)	716
BseXI	GCAGC	3 cut(s)	162, 353, 803
BsgI	GTGCAG	1 cut(s)	558
Bsh1236I	CGCG	2 cut(s)	260, 370
BshFI	GGCC	1 cut(s)	322
BshNI	GGYRCC	2 cut(s)	313, 399
BshTI	ACCGGT	1 cut(s)	396
BsiSI	CCGG	3 cut(s)	337, 397, 635
BslFI	GGGAC	1 cut(s)	269
BslI	CCNNNNNNNGG	3 cut(s)	49, 216, 310
BsmAI	GTCTC	1 cut(s)	491
BsmFI	GGGAC	1 cut(s)	269
BsmI	GAATGC	1 cut(s)	475
BsnI	GGCC	1 cut(s)	322
Bso31I	GGTCTC	1 cut(s)	491
Bsp143I	GATC	5 cut(s)	103, 378, 610, 691, 733
BspACI	CCGC	4 cut(s)	210, 260, 406, 512
BspANI	GGCC	1 cut(s)	322
BspFNI	CGCG	2 cut(s)	260, 370
BspHI	TCATGA	1 cut(s)	649
BspLI	GGNNCC	3 cut(s)	315, 335, 401
BspMAI	CTGCAG	1 cut(s)	180
BspMI	ACCTGC	1 cut(s)	411
BspPI	GGATC	1 cut(s)	605
BspT107I	GGYRCC	2 cut(s)	313, 399
BspTI	CTTAAG	1 cut(s)	764
BspTNI	GGTCTC	1 cut(s)	491
BsrDI	GCAATG	1 cut(s)	462
BsrFI	RCCGGY	1 cut(s)	396
BssAI	RCCGGY	1 cut(s)	396
BssECI	CCNNGG	1 cut(s)	317
BssMI	GATC	5 cut(s)	103, 378, 610, 691, 733
BssSI	CACGAG	1 cut(s)	107
Bst2BI	CACGAG	1 cut(s)	107
Bst2UI	CCWGG	1 cut(s)	318
Bst4CI	ACNGT	3 cut(s)	291, 715, 761
Bst6I	CTCTTC	1 cut(s)	742
BstAFI	CTTAAG	1 cut(s)	764
BstAPI	GCANNNNNTGC	1 cut(s)	800
BstC8I	GCNNGC	2 cut(s)	366, 453
BstF5I	GGATG	1 cut(s)	631
BstFNI	CGCG	2 cut(s)	260, 370
BstHHI	GCGC	1 cut(s)	370
BstKTI	GATC	5 cut(s)	106, 381, 613, 694, 736
BstMAI	GTCTC	1 cut(s)	491
BstMBI	GATC	5 cut(s)	103, 378, 610, 691, 733
BstMWI	GCNNNNNNNGC	3 cut(s)	14, 374, 800
BstNI	CCWGG	1 cut(s)	318
BstNSI	RCATGY	1 cut(s)	817
BstPAI	GACNNNNGTC	1 cut(s)	870
BstSCI	CCNGG	2 cut(s)	316, 336
BstSFI	CTRYAG	2 cut(s)	18, 176
BstUI	CGCG	2 cut(s)	260, 370
BstV1I	GCAGC	3 cut(s)	162, 353, 803
BstV2I	GAAGAC	1 cut(s)	623
BstX2I	RGATCY	1 cut(s)	610
BstYI	RGATCY	1 cut(s)	610
BsuI	GTATCC	1 cut(s)	346
BsuRI	GGCC	1 cut(s)	322
BtsCI	GGATG	1 cut(s)	631
BtsI	GCAGTG	1 cut(s)	470
BtsIMutI	CAGTG	1 cut(s)	470
BveI	ACCTGC	1 cut(s)	411
Cac8I	GCNNGC	2 cut(s)	366, 453
CciI	TCATGA	1 cut(s)	649
CfoI	GCGC	1 cut(s)	370
Cfr10I	RCCGGY	1 cut(s)	396
Cfr13I	GGNCC	1 cut(s)	320
CseI	GACGC	2 cut(s)	227, 266
Csp6I	GTAC	5 cut(s)	151, 292, 301, 394, 400
CspAI	ACCGGT	1 cut(s)	396
CviAII	CATG	4 cut(s)	386, 547, 650, 814
CviJI	RGCY	8 cut(s)	17, 185, 225, 322, 334, 344, 364, 601
CviKI_1	RGCY	8 cut(s)	17, 185, 225, 322, 334, 344, 364, 601
CviQI	GTAC	5 cut(s)	151, 292, 301, 394, 400
DpnI	GATC	5 cut(s)	105, 380, 612, 693, 735
DpnII	GATC	5 cut(s)	103, 378, 610, 691, 733
DriI	GACNNNNNGTC	1 cut(s)	556
Eam1104I	CTCTTC	1 cut(s)	742
Eam1105I	GACNNNNNGTC	1 cut(s)	556
EarI	CTCTTC	1 cut(s)	742
Eco31I	GGTCTC	1 cut(s)	491
EcoRII	CCWGG	1 cut(s)	316
EcoT22I	ATGCAT	2 cut(s)	633, 805
FaeI	CATG	4 cut(s)	389, 550, 653, 817
FalI	AAGNNNNNCTT	2 cut(s)	739, 771
FaqI	GGGAC	1 cut(s)	269
FatI	CATG	4 cut(s)	385, 546, 649, 813
FbaI	TGATCA	1 cut(s)	103
FblI	GTMKAC	1 cut(s)	864
Fnu4HI	GCNGC	5 cut(s)	176, 210, 342, 407, 792
FokI	GGATG	1 cut(s)	618
Fsp4HI	GCNGC	5 cut(s)	176, 210, 342, 407, 792
GlaI	GCGC	1 cut(s)	369
GluI	GCNGC	5 cut(s)	176, 210, 342, 407, 792
GsuI	CTGGAG	1 cut(s)	263
HaeIII	GGCC	1 cut(s)	322
HapII	CCGG	3 cut(s)	337, 397, 635
HgaI	GACGC	2 cut(s)	227, 266
HhaI	GCGC	1 cut(s)	370
Hin1II	CATG	4 cut(s)	389, 550, 653, 817
Hin6I	GCGC	1 cut(s)	368
HinP1I	GCGC	1 cut(s)	368
HincII	GTYRAC	3 cut(s)	90, 99, 327
HindII	GTYRAC	3 cut(s)	90, 99, 327
HindIII	AAGCTT	1 cut(s)	362
HinfI	GANTC	1 cut(s)	524
HpaII	CCGG	3 cut(s)	337, 397, 635
Hpy166II	GTNNAC	5 cut(s)	90, 99, 327, 528, 865
Hpy188I	TCNGA	3 cut(s)	463, 615, 675
Hpy8I	GTNNAC	5 cut(s)	90, 99, 327, 528, 865
Hpy99I	CGWCG	2 cut(s)	97, 209
HpyAV	CCTTC	3 cut(s)	211, 571, 763
HpyCH4III	ACNGT	3 cut(s)	291, 715, 761
HpyCH4IV	ACGT	3 cut(s)	46, 92, 567
HpyCH4V	TGCA	7 cut(s)	178, 475, 539, 555, 631, 779, 803
HpyF10VI	GCNNNNNNNGC	3 cut(s)	14, 374, 800
HpySE526I	ACGT	3 cut(s)	46, 92, 567
Hsp92II	CATG	4 cut(s)	389, 550, 653, 817
HspAI	GCGC	1 cut(s)	368
KpnI	GGTACC	1 cut(s)	403
Ksp22I	TGATCA	1 cut(s)	103
Kzo9I	GATC	5 cut(s)	103, 378, 610, 691, 733
LmnI	GCTCC	2 cut(s)	339, 502
Lsp1109I	GCAGC	3 cut(s)	162, 353, 803
LweI	GCATC	5 cut(s)	121, 484, 618, 640, 790
MaeII	ACGT	3 cut(s)	46, 92, 567
MaeIII	GTNAC	1 cut(s)	639
MalI	GATC	5 cut(s)	105, 380, 612, 693, 735
MboI	GATC	5 cut(s)	103, 378, 610, 691, 733
MboII	GAAGA	8 cut(s)	35, 38, 140, 151, 189, 440, 628, 759
MfeI	CAATTG	1 cut(s)	561
MflI	RGATCY	1 cut(s)	610
MluCI	AATT	7 cut(s)	155, 268, 435, 443, 561, 589, 738
MlyI	GAGTC	1 cut(s)	533
MmeI	TCCRAC	2 cut(s)	85, 724
MnlI	CCTC	5 cut(s)	175, 694, 712, 743, 874
Mph1103I	ATGCAT	2 cut(s)	633, 805
MroXI	GAANNNNTTC	2 cut(s)	435, 581
MseI	TTAA	2 cut(s)	765, 886
MslI	CAYNNNNRTG	1 cut(s)	480
MspCI	CTTAAG	1 cut(s)	764
MspI	CCGG	3 cut(s)	337, 397, 635
MspR9I	CCNGG	2 cut(s)	318, 338
MunI	CAATTG	1 cut(s)	561
Mva1269I	GAATGC	1 cut(s)	475
MvaI	CCWGG	1 cut(s)	318
MvnI	CGCG	2 cut(s)	260, 370
MwoI	GCNNNNNNNGC	3 cut(s)	14, 374, 800
NciI	CCSGG	1 cut(s)	338
NdeII	GATC	5 cut(s)	103, 378, 610, 691, 733
NlaIII	CATG	4 cut(s)	389, 550, 653, 817
NlaIV	GGNNCC	3 cut(s)	315, 335, 401
NmeAIII	GCCGAG	1 cut(s)	238
NmuCI	GTSAC	1 cut(s)	639
NsiI	ATGCAT	2 cut(s)	633, 805
NspI	RCATGY	1 cut(s)	817
PagI	TCATGA	1 cut(s)	649
PciI	ACATGT	1 cut(s)	813
PcsI	WCGNNNNNNNCGW	1 cut(s)	210
PctI	GAATGC	1 cut(s)	475
PdmI	GAANNNNTTC	2 cut(s)	435, 581
PflFI	GACNNNGTC	1 cut(s)	56
PinAI	ACCGGT	1 cut(s)	396
PkrI	GCNGC	5 cut(s)	177, 211, 343, 408, 793
PleI	GAGTC	1 cut(s)	532
PpsI	GAGTC	1 cut(s)	532
PscI	ACATGT	1 cut(s)	813
PshAI	GACNNNNGTC	1 cut(s)	870
PsiI	TTATAA	1 cut(s)	728
Psp6I	CCWGG	1 cut(s)	316
PspGI	CCWGG	1 cut(s)	316
PspN4I	GGNNCC	3 cut(s)	315, 335, 401
PspPI	GGNCC	1 cut(s)	320
PstI	CTGCAG	1 cut(s)	180
PsuI	RGATCY	1 cut(s)	610
PsyI	GACNNNGTC	1 cut(s)	56
RsaI	GTAC	5 cut(s)	152, 293, 302, 395, 401
RsaNI	GTAC	5 cut(s)	151, 292, 301, 394, 400
RseI	CAYNNNNRTG	1 cut(s)	480
SaqAI	TTAA	2 cut(s)	765, 886
SatI	GCNGC	5 cut(s)	176, 210, 342, 407, 792
Sau3AI	GATC	5 cut(s)	103, 378, 610, 691, 733
Sau96I	GGNCC	1 cut(s)	320
SchI	GAGTC	1 cut(s)	533
ScrFI	CCNGG	2 cut(s)	318, 338
SfaNI	GCATC	5 cut(s)	121, 484, 618, 640, 790
SfcI	CTRYAG	2 cut(s)	18, 176
SmiMI	CAYNNNNRTG	1 cut(s)	480
SmlI	CTYRAG	1 cut(s)	764
SmoI	CTYRAG	1 cut(s)	764
Sse9I	AATT	7 cut(s)	155, 268, 435, 443, 561, 589, 738
SsiI	CCGC	4 cut(s)	210, 260, 406, 512
StyD4I	CCNGG	2 cut(s)	316, 336
TaaI	ACNGT	3 cut(s)	291, 715, 761
TaiI	ACGT	3 cut(s)	49, 95, 570
TaqI	TCGA	5 cut(s)	159, 439, 682, 690, 874
TasI	AATT	7 cut(s)	155, 268, 435, 443, 561, 589, 738
TauI	GCSGC	2 cut(s)	212, 409
Tru1I	TTAA	2 cut(s)	765, 886
Tru9I	TTAA	2 cut(s)	765, 886
TscAI	CASTG	1 cut(s)	477
TseFI	GTSAC	1 cut(s)	639
TseI	GCWGC	3 cut(s)	175, 341, 791
Tsp45I	GTSAC	1 cut(s)	639
TspDTI	ATGAA	3 cut(s)	535, 638, 666
TspGWI	ACGGA	1 cut(s)	371
TspRI	CASTG	1 cut(s)	477
Tth111I	GACNNNGTC	1 cut(s)	56
Vha464I	CTTAAG	1 cut(s)	764
XapI	RAATTY	5 cut(s)	155, 268, 435, 443, 589
XceI	RCATGY	1 cut(s)	817
XmiI	GTMKAC	1 cut(s)	864
XmnI	GAANNNNTTC	2 cut(s)	435, 581
Zsp2I	ATGCAT	2 cut(s)	633, 805

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.