Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0004428

Physical Location & Seq

Genomic Coordinates Reverse (-)

58541256 .. 58542734

Gene Structure 1479 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00265090.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1479 bp

ATGAAACGAGGGTACTCAGAATCGTCCTCCACCTCTCTCGGGCCACCTCAATCCAGGTTCAAGCACAACCCAGAAAATGACCTCTTCGAAGATGAAAGCACAAACATTTTCGCACGCAAAGTGGCTGAACATTACAGTGATCAAACTCTGGAAGAAGGAGAAATTAGTCAAACCATGGAAGTGGCCGAGCATTACAGTGATCAAACTTTGGAAGAAGGAGAAATTAGTCCAACCATGGAAGCACAACCCCAAGAAACCATGAACGCAGTGAAGCAAAGCTTATTACAGACCGAGTCAACCTCGAAGGAGCCGAGCCTTGATGAAGTGGAGGAGATCATACGCTACAAATTCAGGAACAAGAAGTTACTAGAGGAAGCCTTTACGCATTCTTCTTTTACTTGCGGCTTTTCCTATGAGCGTTTGGAGTACGTAGGCGATGCGGTGCTCGGTTTATTGTTTTGCCGGGCACATTATTCTCTGTACCCGGATTTAGCTCCGGGAAAGCTGACCCGCCTCCGGGCTGCCAACGTCGATACCGAGAAGCTTGCACGTGTGGCACTCCGGCACGGCTTCCACCGCTACCTCCGCCACAGGAAACCTTACCTTGTAGATCAAATTCAAGAATTTTCACGAGCGGTTATTGAGTATCCATTGCACTCAAATGGTCTTATTGACGCTCCGAAAGACCTTGCAGATGTTGTAGAGTCCACTATTGGGGCGGTTTTCATGGACTGCAACTCCATAGAGGTTGTGTGGCATGTGTTTAAGGGCTTGCTAGAGCCGATCATAACTCAGGAGACGATACAAATACATCCAAACACACAGCTTCTTGAGATGTGTCAAAAGAACCACCGGAGTCTCAAGTATGTAGATTCATGGGAGAAGGACTATAGTGTTGGGTGTGTCATTGATGGTCAGATTGTTGGAAGAGGTACCTATAGTCTTCGGAAGGACGTTGCGCTAAACCGAGCAGCAAAAGATGCATTGGACAATAGGTGGAAGTGGATGGAGAATAATGCTACTGCCATTGCTCAACGTGTGACGGAGGAGCCTTCGGAACCGGTGCTAGTGAAGAATATGAACATGGAGAATAGTGATGAAGACGCTATTAGCTCTGTGGAGCAGGCTGAGGAACAAAGTGTCTGTTCTTTGGTGCCCGTTACTCCAGGAAAGAAAGATATGAAGGAGGATCTAGAGACAAATGACACTTCTGTTCAAGGTCCGAATGTTTTGAGTGAAGAGAGTCAAGAGACTACTTCATATAATGAGACTAATGTTACTCCTCCTCGAGGTCAGATAGAAATGCATGCCATAAATTCTCTTCTGGGGAATGCGATTGGTGATGAGTTTATTGGACTCTCTAGAGGACAACGAAAGCGCCTCCGCAAAAGACAACGGAAGATTTCGGCTAACACAACAATAATAACTCCGGAGACAATACCACTTCATTCAAACACACAGTTTCTTGAGATATGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ribonucleas_3_3 Ribonuclease_3 dsrm

Protein Analysis

492

Amino Acids

55.78

Weight (kDa)

5.14

Isoelectric Point (pI)

45.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ribonucleas_3_3	PF14622	119 - 244	2.9e-13	Ribonuclease-III-like
Ribonuclease_3	PF00636	139 - 245	8.5e-21	Ribonuclease III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004428)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15417 AT4G15417
fragaria_vesca	FvH4_5g08550 FvH4_6g42650
malus_domestica	MD09G1111700.v1.1 MD17G1102000.v1.1
prunus_persica	Prupe.3G213200_v2.0.a1
pyrus_communis	pycom09g03630 pycom17g09640
rosa_chinensis	RchiOBHm_Chr2g0158531 RchiOBHm_Chr7g0189841
rosa_laevigata	RLG00000004582 RLG00000021094
rosa_multiflora	Rmu_co8278635.1_g000001 Rmu_sc0000512.1_g000018 Rmu_sc0005896.1_g000003 Rmu_sc0005896.1_g000004 Rmu_sc0017496.1_g000006
rosa_roxburghii	Rroxscaffold_2G00091270 Rroxscaffold_3G00265090
rosa_rugosa	Rorug02G0469800 Rorug06G0500300
rosa_samantha	Rh2AG535800 Rh2BG549000 Rh2CG519500 Rh2DG558700 Rh7AG105600 Rh7BG108400 Rh7CG110400 Rh7DG108700
rosa_wichuraiana	Rw2G044370 Rw7G009150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	1287
Acc65I	GGTACC	1 cut(s)	932
AccB1I	GGYRCC	2 cut(s)	932, 1153
AccBSI	CCGCTC	1 cut(s)	635
AccIII	TCCGGA	1 cut(s)	1429
AciI	CCGC	8 cut(s)	402, 440, 511, 577, 586, 635, 719, 1384
AclWI	GGATC	1 cut(s)	1197
AcoI	YGGCCR	1 cut(s)	183
AcsI	RAATTY	4 cut(s)	347, 615, 623, 1315
AcvI	CACGTG	1 cut(s)	551
AfaI	GTAC	4 cut(s)	14, 428, 482, 934
AfiI	CCNNNNNNNGG	5 cut(s)	39, 516, 517, 714, 1289
AflIII	ACRYGT	2 cut(s)	550, 1036
AgeI	ACCGGT	1 cut(s)	1060
AgsI	TTSAA	4 cut(s)	61, 620, 1217, 1452
AjnI	CCWGG	2 cut(s)	53, 1165
AluBI	AGCT	6 cut(s)	279, 494, 505, 544, 826, 1113
AluI	AGCT	6 cut(s)	279, 494, 505, 544, 826, 1113
Alw21I	GWGCWC	1 cut(s)	447
Alw26I	GTCTC	6 cut(s)	791, 863, 1190, 1244, 1262, 1427
AlwI	GGATC	1 cut(s)	1197
Ama87I	CYCGRG	2 cut(s)	38, 1287
Aor13HI	TCCGGA	1 cut(s)	1429
AoxI	GGCC	2 cut(s)	41, 183
ApeKI	GCWGC	2 cut(s)	521, 971
ApoI	RAATTY	4 cut(s)	347, 615, 623, 1315
AsiGI	ACCGGT	1 cut(s)	1060
Asp718I	GGTACC	1 cut(s)	932
AspLEI	GCGC	2 cut(s)	961, 1380
AspS9I	GGNCC	2 cut(s)	41, 1220
AsuC2I	CCSGG	4 cut(s)	464, 485, 498, 518
AsuHPI	GGTGA	1 cut(s)	1352
AsuII	TTCGAA	1 cut(s)	87
AvaI	CYCGRG	2 cut(s)	38, 1287
AvaII	GGWCC	1 cut(s)	1220
BaeGI	GKGCMC	2 cut(s)	469, 1158
BanI	GGYRCC	2 cut(s)	932, 1153
BauI	CACGAG	1 cut(s)	630
BbrPI	CACGTG	1 cut(s)	551
BbsI	GAAGAC	2 cut(s)	935, 1107
Bbv12I	GWGCWC	1 cut(s)	447
BbvCI	CCTCAGC	1 cut(s)	1128
BbvI	GCAGC	2 cut(s)	508, 983
BccI	CCATC	2 cut(s)	905, 1000
BceAI	ACGGC	1 cut(s)	583
BcgI	CGANNNNNNTGC	2 cut(s)	527, 561
BciT130I	CCWGG	2 cut(s)	55, 1167
BciVI	GTATCC	1 cut(s)	657
BclI	TGATCA	2 cut(s)	139, 199
BcnI	CCSGG	4 cut(s)	464, 485, 498, 518
BcoDI	GTCTC	6 cut(s)	791, 863, 1190, 1244, 1262, 1427
BfaI	CTAG	5 cut(s)	368, 776, 1067, 1193, 1362
BfmI	CTRYAG	2 cut(s)	889, 937
BfoI	RGCGCY	1 cut(s)	1381
BfuI	GTATCC	1 cut(s)	657
BisI	GCNGC	3 cut(s)	403, 522, 972
BlsI	GCNGC	3 cut(s)	404, 523, 973
Bme1390I	CCNGG	6 cut(s)	55, 464, 485, 498, 518, 1167
Bme18I	GGWCC	1 cut(s)	1220
BmeT110I	CYCGRG	2 cut(s)	38, 1287
BmgT120I	GGNCC	2 cut(s)	41, 1220
BmiI	GGNNCC	5 cut(s)	309, 934, 1050, 1059, 1155
BmrFI	CCNGG	6 cut(s)	55, 464, 485, 498, 518, 1167
BmsI	GCATC	2 cut(s)	427, 970
BpiI	GAAGAC	2 cut(s)	935, 1107
BplI	GAGNNNNNCTC	2 cut(s)	284, 316
BpmI	CTGGAG	1 cut(s)	1149
Bpu10I	CCTNAGC	1 cut(s)	1128
Bpu14I	TTCGAA	1 cut(s)	87
BpuEI	CTTGAG	2 cut(s)	845, 851
BpuMI	CCSGG	4 cut(s)	464, 485, 498, 518
BsaAI	YACGTR	2 cut(s)	430, 551
BsaJI	CCNNGG	2 cut(s)	174, 234
BsaWI	WCCGGW	3 cut(s)	852, 1060, 1429
BsaXI	ACNNNNNCTCC	2 cut(s)	722, 752
Bsc4I	CCNNNNNNNGG	5 cut(s)	39, 516, 517, 714, 1289
Bse118I	RCCGGY	1 cut(s)	1060
Bse3DI	GCAATG	2 cut(s)	650, 1026
BseAI	TCCGGA	1 cut(s)	1429
BseBI	CCWGG	2 cut(s)	55, 1167
BseDI	CCNNGG	2 cut(s)	174, 234
BseGI	GGATG	2 cut(s)	811, 1011
BseLI	CCNNNNNNNGG	5 cut(s)	39, 516, 517, 714, 1289
BseMI	GCAATG	2 cut(s)	650, 1026
BseMII	CTCAG	3 cut(s)	30, 806, 1119
BseRI	GAGGAG	4 cut(s)	344, 1061, 1272, 1275
BseSI	GKGCMC	2 cut(s)	469, 1158
BseXI	GCAGC	2 cut(s)	508, 983
BshFI	GGCC	2 cut(s)	43, 185
BshNI	GGYRCC	2 cut(s)	932, 1153
BshTI	ACCGGT	1 cut(s)	1060
BsiHKAI	GWGCWC	1 cut(s)	447
BsiHKCI	CYCGRG	2 cut(s)	38, 1287
BsiSI	CCGG	8 cut(s)	463, 485, 497, 517, 562, 853, 1061, 1430
BslI	CCNNNNNNNGG	5 cut(s)	39, 516, 517, 714, 1289
BsmAI	GTCTC	6 cut(s)	791, 863, 1190, 1244, 1262, 1427
BsmBI	CGTCTC	1 cut(s)	791
BsmI	GAATGC	2 cut(s)	385, 1336
BsnI	GGCC	2 cut(s)	43, 185
BsoBI	CYCGRG	2 cut(s)	38, 1287
Bsp119I	TTCGAA	1 cut(s)	87
Bsp1286I	GDGCHC	3 cut(s)	447, 469, 1158
Bsp13I	TCCGGA	1 cut(s)	1429
Bsp143I	GATC	6 cut(s)	139, 199, 333, 610, 783, 1189
Bsp19I	CCATGG	2 cut(s)	174, 234
BspACI	CCGC	8 cut(s)	402, 440, 511, 577, 586, 635, 719, 1384
BspANI	GGCC	2 cut(s)	43, 185
BspCNI	CTCAG	3 cut(s)	29, 805, 1120
BspEI	TCCGGA	1 cut(s)	1429
BspLI	GGNNCC	5 cut(s)	309, 934, 1050, 1059, 1155
BspPI	GGATC	1 cut(s)	1197
BspT104I	TTCGAA	1 cut(s)	87
BspT107I	GGYRCC	2 cut(s)	932, 1153
BsrBI	CCGCTC	1 cut(s)	635
BsrDI	GCAATG	2 cut(s)	650, 1026
BsrFI	RCCGGY	1 cut(s)	1060
BssAI	RCCGGY	1 cut(s)	1060
BssECI	CCNNGG	2 cut(s)	174, 234
BssMI	GATC	6 cut(s)	139, 199, 333, 610, 783, 1189
BssSI	CACGAG	1 cut(s)	630
BssT1I	CCWWGG	2 cut(s)	174, 234
Bst2BI	CACGAG	1 cut(s)	630
Bst2UI	CCWGG	2 cut(s)	55, 1167
Bst4CI	ACNGT	3 cut(s)	137, 197, 1461
Bst6I	CTCTTC	4 cut(s)	89, 922, 1233, 1326
BstAPI	GCANNNNNTGC	1 cut(s)	980
BstBAI	YACGTR	2 cut(s)	430, 551
BstBI	TTCGAA	1 cut(s)	87
BstC8I	GCNNGC	5 cut(s)	115, 546, 773, 1125, 1308
BstDEI	CTNAG	3 cut(s)	16, 792, 1128
BstDSI	CCRYGG	2 cut(s)	174, 234
BstENI	CCTNNNNNAGG	1 cut(s)	1287
BstF5I	GGATG	2 cut(s)	811, 1011
BstH2I	RGCGCY	1 cut(s)	1381
BstHHI	GCGC	2 cut(s)	961, 1380
BstKTI	GATC	6 cut(s)	142, 202, 336, 613, 786, 1192
BstMAI	GTCTC	6 cut(s)	791, 863, 1190, 1244, 1262, 1427
BstMBI	GATC	6 cut(s)	139, 199, 333, 610, 783, 1189
BstMWI	GCNNNNNNNGC	4 cut(s)	554, 576, 585, 980
BstNI	CCWGG	2 cut(s)	55, 1167
BstNSI	RCATGY	2 cut(s)	761, 1310
BstSCI	CCNGG	6 cut(s)	53, 462, 483, 496, 516, 1165
BstSFI	CTRYAG	2 cut(s)	889, 937
BstSLI	GKGCMC	2 cut(s)	469, 1158
BstSNI	TACGTA	1 cut(s)	430
BstV1I	GCAGC	2 cut(s)	508, 983
BstV2I	GAAGAC	2 cut(s)	935, 1107
BstX2I	RGATCY	1 cut(s)	1189
BstXI	CCANNNNNNTGG	1 cut(s)	181
BstYI	RGATCY	1 cut(s)	1189
BsuI	GTATCC	1 cut(s)	657
BsuRI	GGCC	2 cut(s)	43, 185
BtgI	CCRYGG	2 cut(s)	174, 234
BtgZI	GCGATG	1 cut(s)	450
BtsCI	GGATG	2 cut(s)	811, 1011
BtsI	GCAGTG	1 cut(s)	273
BtsIMutI	CAGTG	3 cut(s)	142, 202, 273
Cac8I	GCNNGC	5 cut(s)	115, 546, 773, 1125, 1308
CfoI	GCGC	2 cut(s)	961, 1380
Cfr10I	RCCGGY	1 cut(s)	1060
Cfr13I	GGNCC	2 cut(s)	41, 1220
CseI	GACGC	2 cut(s)	683, 1112
Csp6I	GTAC	4 cut(s)	13, 427, 481, 933
CspAI	ACCGGT	1 cut(s)	1060
CviAII	CATG	8 cut(s)	175, 235, 259, 727, 758, 876, 1084, 1307
CviQI	GTAC	4 cut(s)	13, 427, 481, 933
DdeI	CTNAG	3 cut(s)	16, 792, 1128
DpnI	GATC	6 cut(s)	141, 201, 335, 612, 785, 1191
DpnII	GATC	6 cut(s)	139, 199, 333, 610, 783, 1189
EaeI	YGGCCR	1 cut(s)	183
Eam1104I	CTCTTC	4 cut(s)	89, 922, 1233, 1326
EarI	CTCTTC	4 cut(s)	89, 922, 1233, 1326
EciI	GGCGGA	1 cut(s)	575
Eco105I	TACGTA	1 cut(s)	430
Eco130I	CCWWGG	2 cut(s)	174, 234
Eco47I	GGWCC	1 cut(s)	1220
Eco72I	CACGTG	1 cut(s)	551
Eco88I	CYCGRG	2 cut(s)	38, 1287
EcoNI	CCTNNNNNAGG	1 cut(s)	1287
EcoRII	CCWGG	2 cut(s)	53, 1165
EcoT14I	CCWWGG	2 cut(s)	174, 234
EcoT22I	ATGCAT	2 cut(s)	985, 1308
ErhI	CCWWGG	2 cut(s)	174, 234
Esp3I	CGTCTC	1 cut(s)	791
FaeI	CATG	8 cut(s)	178, 238, 262, 730, 761, 879, 1087, 1310
FalI	AAGNNNNNCTT	2 cut(s)	263, 295
FatI	CATG	8 cut(s)	174, 234, 258, 726, 757, 875, 1083, 1306
FauI	CCCGC	1 cut(s)	518
FbaI	TGATCA	2 cut(s)	139, 199
Fnu4HI	GCNGC	3 cut(s)	403, 522, 972
FokI	GGATG	2 cut(s)	798, 1018
Fsp4HI	GCNGC	3 cut(s)	403, 522, 972
FspBI	CTAG	5 cut(s)	368, 776, 1067, 1193, 1362
GlaI	GCGC	2 cut(s)	960, 1379
GluI	GCNGC	3 cut(s)	403, 522, 972
GsuI	CTGGAG	1 cut(s)	1149
HaeII	RGCGCY	1 cut(s)	1381
HaeIII	GGCC	2 cut(s)	43, 185
HapII	CCGG	8 cut(s)	463, 485, 497, 517, 562, 853, 1061, 1430
HgaI	GACGC	2 cut(s)	683, 1112
HhaI	GCGC	2 cut(s)	961, 1380
Hin1II	CATG	8 cut(s)	178, 238, 262, 730, 761, 879, 1087, 1310
Hin6I	GCGC	2 cut(s)	959, 1378
HinP1I	GCGC	2 cut(s)	959, 1378
HincII	GTYRAC	1 cut(s)	297
HindII	GTYRAC	1 cut(s)	297
HindIII	AAGCTT	2 cut(s)	277, 542
HinfI	GANTC	7 cut(s)	20, 293, 704, 856, 872, 1243, 1356
HpaII	CCGG	8 cut(s)	463, 485, 497, 517, 562, 853, 1061, 1430
HphI	GGTGA	1 cut(s)	1352
Hpy166II	GTNNAC	2 cut(s)	297, 708
Hpy188I	TCNGA	7 cut(s)	19, 681, 918, 948, 1057, 1224, 1296
Hpy8I	GTNNAC	2 cut(s)	297, 708
Hpy99I	CGWCG	1 cut(s)	533
HpyAV	CCTTC	7 cut(s)	149, 209, 298, 877, 943, 1062, 1177
HpyCH4III	ACNGT	3 cut(s)	137, 197, 1461
HpyCH4IV	ACGT	5 cut(s)	429, 528, 550, 954, 1036
HpyCH4V	TGCA	6 cut(s)	548, 655, 692, 735, 983, 1306
HpyF10VI	GCNNNNNNNGC	4 cut(s)	554, 576, 585, 980
HpyF3I	CTNAG	3 cut(s)	16, 792, 1128
HpySE526I	ACGT	5 cut(s)	429, 528, 550, 954, 1036
Hsp92II	CATG	8 cut(s)	178, 238, 262, 730, 761, 879, 1087, 1310
HspAI	GCGC	2 cut(s)	959, 1378
Kpn2I	TCCGGA	1 cut(s)	1429
KpnI	GGTACC	1 cut(s)	936
Ksp22I	TGATCA	2 cut(s)	139, 199
Kzo9I	GATC	6 cut(s)	139, 199, 333, 610, 783, 1189
LmnI	GCTCC	5 cut(s)	307, 499, 682, 1048, 1120
Lsp1109I	GCAGC	2 cut(s)	508, 983
LweI	GCATC	2 cut(s)	427, 970
MaeI	CTAG	5 cut(s)	368, 776, 1067, 1193, 1362
MaeII	ACGT	5 cut(s)	429, 528, 550, 954, 1036
MaeIII	GTNAC	4 cut(s)	363, 1039, 1159, 1276
MalI	GATC	6 cut(s)	141, 201, 335, 612, 785, 1191
MbiI	CCGCTC	1 cut(s)	635
MboI	GATC	6 cut(s)	139, 199, 333, 610, 783, 1189
MflI	RGATCY	1 cut(s)	1189
MhlI	GDGCHC	3 cut(s)	447, 469, 1158
MluCI	AATT	6 cut(s)	162, 222, 347, 615, 623, 1315
MlyI	GAGTC	5 cut(s)	302, 713, 865, 1252, 1350
MmeI	TCCRAC	2 cut(s)	254, 904
Mph1103I	ATGCAT	2 cut(s)	985, 1308
MroI	TCCGGA	1 cut(s)	1429
MseI	TTAA	2 cut(s)	765, 1477
MslI	CAYNNNNRTG	4 cut(s)	135, 179, 195, 660
MspI	CCGG	8 cut(s)	463, 485, 497, 517, 562, 853, 1061, 1430
MspR9I	CCNGG	6 cut(s)	55, 464, 485, 498, 518, 1167
Mva1269I	GAATGC	2 cut(s)	385, 1336
MvaI	CCWGG	2 cut(s)	55, 1167
MwoI	GCNNNNNNNGC	4 cut(s)	554, 576, 585, 980
NciI	CCSGG	4 cut(s)	464, 485, 498, 518
NcoI	CCATGG	2 cut(s)	174, 234
NdeII	GATC	6 cut(s)	139, 199, 333, 610, 783, 1189
NlaIII	CATG	8 cut(s)	178, 238, 262, 730, 761, 879, 1087, 1310
NlaIV	GGNNCC	5 cut(s)	309, 934, 1050, 1059, 1155
NmeAIII	GCCGAG	2 cut(s)	211, 336
NmuCI	GTSAC	1 cut(s)	1039
NsiI	ATGCAT	2 cut(s)	985, 1308
NspI	RCATGY	2 cut(s)	761, 1310
NspV	TTCGAA	1 cut(s)	87
PaeI	GCATGC	1 cut(s)	1310
PaeR7I	CTCGAG	1 cut(s)	1287
PcsI	WCGNNNNNNNCGW	2 cut(s)	308, 534
PctI	GAATGC	2 cut(s)	385, 1336
PfeI	GAWTC	2 cut(s)	20, 872
PflFI	GACNNNGTC	1 cut(s)	292
PfoI	TCCNGGA	2 cut(s)	496, 1165
PinAI	ACCGGT	1 cut(s)	1060
PkrI	GCNGC	3 cut(s)	404, 523, 973
PleI	GAGTC	5 cut(s)	301, 712, 864, 1251, 1350
PmaCI	CACGTG	1 cut(s)	551
PmlI	CACGTG	1 cut(s)	551
PpsI	GAGTC	5 cut(s)	301, 712, 864, 1251, 1350
Ppu21I	YACGTR	2 cut(s)	430, 551
Psp6I	CCWGG	2 cut(s)	53, 1165
PspCI	CACGTG	1 cut(s)	551
PspGI	CCWGG	2 cut(s)	53, 1165
PspN4I	GGNNCC	5 cut(s)	309, 934, 1050, 1059, 1155
PspPI	GGNCC	2 cut(s)	41, 1220
PspXI	VCTCGAGB	1 cut(s)	1287
PsuI	RGATCY	1 cut(s)	1189
PsyI	GACNNNGTC	1 cut(s)	292
RsaI	GTAC	4 cut(s)	14, 428, 482, 934
RsaNI	GTAC	4 cut(s)	13, 427, 481, 933
RseI	CAYNNNNRTG	4 cut(s)	135, 179, 195, 660
SaqAI	TTAA	2 cut(s)	765, 1477
SatI	GCNGC	3 cut(s)	403, 522, 972
Sau3AI	GATC	6 cut(s)	139, 199, 333, 610, 783, 1189
Sau96I	GGNCC	2 cut(s)	41, 1220
SchI	GAGTC	5 cut(s)	302, 713, 865, 1252, 1350
ScrFI	CCNGG	6 cut(s)	55, 464, 485, 498, 518, 1167
SduI	GDGCHC	3 cut(s)	447, 469, 1158
SfaNI	GCATC	2 cut(s)	427, 970
SfcI	CTRYAG	2 cut(s)	889, 937
Sfr274I	CTCGAG	1 cut(s)	1287
SfuI	TTCGAA	1 cut(s)	87
SinI	GGWCC	1 cut(s)	1220
SlaI	CTCGAG	1 cut(s)	1287
SmiMI	CAYNNNNRTG	4 cut(s)	135, 179, 195, 660
SmlI	CTYRAG	4 cut(s)	830, 860, 1287, 1466
SmoI	CTYRAG	4 cut(s)	830, 860, 1287, 1466
SnaBI	TACGTA	1 cut(s)	430
SphI	GCATGC	1 cut(s)	1310
Sse9I	AATT	6 cut(s)	162, 222, 347, 615, 623, 1315
SsiI	CCGC	8 cut(s)	402, 440, 511, 577, 586, 635, 719, 1384
SspMI	CTAG	5 cut(s)	368, 776, 1067, 1193, 1362
StyD4I	CCNGG	6 cut(s)	53, 462, 483, 496, 516, 1165
StyI	CCWWGG	2 cut(s)	174, 234
TaaI	ACNGT	3 cut(s)	137, 197, 1461
TaiI	ACGT	5 cut(s)	432, 531, 553, 957, 1039
TaqI	TCGA	4 cut(s)	87, 302, 531, 1288
TaqII	GACCGA	1 cut(s)	305
TasI	AATT	6 cut(s)	162, 222, 347, 615, 623, 1315
TauI	GCSGC	1 cut(s)	405
TfiI	GAWTC	2 cut(s)	20, 872
Tru1I	TTAA	2 cut(s)	765, 1477
Tru9I	TTAA	2 cut(s)	765, 1477
TscAI	CASTG	3 cut(s)	142, 202, 273
TseFI	GTSAC	1 cut(s)	1039
TseI	GCWGC	2 cut(s)	521, 971
Tsp45I	GTSAC	1 cut(s)	1039
TspGWI	ACGGA	2 cut(s)	1058, 1411
TspRI	CASTG	3 cut(s)	142, 202, 273
Tth111I	GACNNNGTC	1 cut(s)	292
VpaK11BI	GGWCC	1 cut(s)	1220
XagI	CCTNNNNNAGG	1 cut(s)	1287
XapI	RAATTY	4 cut(s)	347, 615, 623, 1315
XbaI	TCTAGA	2 cut(s)	1192, 1361
XceI	RCATGY	2 cut(s)	761, 1310
XhoI	CTCGAG	1 cut(s)	1287
XspI	CTAG	5 cut(s)	368, 776, 1067, 1193, 1362
Zsp2I	ATGCAT	2 cut(s)	985, 1308

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.