Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0004428

Physical Location & Seq

Genomic Coordinates Forward (+)

8625179 .. 8626612

Gene Structure 1434 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G009150.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1434 bp

ATGAAACGAGGGTACTCCGAATCGTCCTCCACCTCTCTTGAGCCACCTCAATCCAGGTTCAAATACAACCCAGAAAATGACCTCTTGGAAGATGAAAGCACGAACATTTTCGCACGTAAAGTGACTGAACATTACAGTGATCAAACTCTGGAAGAAGGAGAAATTAGTCCAACCATGGAAGTGGCCGAGCATTACAGTGATCAAACTTTGGAAGAAGGAGAAATTAGTCCAACCATGGAAGCACAACCCCAAGAAACCCCTACTGTTCACATGAACGCAGTGAAGCAAAGCTTATTACAGACCGAGTCAACCTCGAAGGAGCCGAGTCTTGATGAAGTGGAGGAGATCATACGCTACAAATTCAGGAACAAGAAGTTACTAGAGGAAGCCTTTACGCATCCCTCTTTTACTTGCGGCTTTTCCTATGAGCGTTTGGAGTACGTAGGCGATGCGGTGCTCGGTTTATTGTTTTGCCGGGCACATTATTCTCTGTACCCGGATTTAGCTCCGGGAAAGCTGACCCGCCTCCGGGCGGCCAACGTCGATACCGAGAAGCTTGCACGTGTGGCACTCCGGCAAGGCTTCCTCCGGTACCTCCGCCACAGGAAACCTTACCTTGTAGATCAAATTCAAGAGTTTTCACGAGCGGTTATTGAGTATCCATTGCACTCAAATGGTCTTATTGACGCTCCAAAAGACCTTGCAGATGTTGTAGAGTCCACTATTGGGGCGGTTTTCATGGACTGCAACTCCATAGAGGTTGTGTGGCATGTGTTTAAGGGCTTGCTAGAGCCGATCATAACTCAGGAGACGATACAGATACATCCAAACACACAGCTTCTTGAGATGTGTCAAAAGAACCGCCGGAGTCTCAAGTATGTAGATTCATGGGAGAAGGACTATAGTGTTGGGTGTGTCATTGATGGTCAGATTGTTGGAAGAGGTACCTATAGTCTTCGGAAGGACGTTGCGCTAAACCGAGCAGCAAAAGATGCATTGGACAATAGGTGGAAGTGGATGGAGAATAATGCTACTGCCATTGCTCAACATGTGACGGAGGAGCCTTCGGAACCGGTGCTAGTGAAGCATATGAACATGGAGAATAGTGATGAAGACGCTATTAGCTCTGTGGAGCAGGCTGAGGAACCAAGTGTCAGTTCTTTGGTGCCAGTTACTCCAGGAAAGAAAGATATGAAGGAGGATCTAGAGACTAATGACACTTCTGTTCAAGGTCCGAATGTTTTGAGTGAAGAGACTACTTCATATAATGAGACTAATGTTACTCCTCCTCGAGGTCAGATAGAAATGCATGCCATAAATTCTCTTCAGGGGAATGGGATTGGTGATGAGTTTATTGGACTCTCTAGAGGACAACGAAAGCGCCTCCGAAAAAGACAACGGAAGATTTCGGCTAAGTGGCTAACACAACAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ribonucleas_3_3 Ribonuclease_3 dsrm

Protein Analysis

477

Amino Acids

54.2

Weight (kDa)

5.18

Isoelectric Point (pI)

48.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ribonucleas_3_3	PF14622	123 - 248	1.1e-11	Ribonuclease-III-like
Ribonuclease_3	PF00636	143 - 249	1.4e-19	Ribonuclease III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004428)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15417 AT4G15417
fragaria_vesca	FvH4_5g08550 FvH4_6g42650
malus_domestica	MD09G1111700.v1.1 MD17G1102000.v1.1
prunus_persica	Prupe.3G213200_v2.0.a1
pyrus_communis	pycom09g03630 pycom17g09640
rosa_chinensis	RchiOBHm_Chr2g0158531 RchiOBHm_Chr7g0189841
rosa_laevigata	RLG00000004582 RLG00000021094
rosa_multiflora	Rmu_co8278635.1_g000001 Rmu_sc0000512.1_g000018 Rmu_sc0005896.1_g000003 Rmu_sc0005896.1_g000004 Rmu_sc0017496.1_g000006
rosa_roxburghii	Rroxscaffold_2G00091270 Rroxscaffold_3G00265090
rosa_rugosa	Rorug02G0469800 Rorug06G0500300
rosa_samantha	Rh2AG535800 Rh2BG549000 Rh2CG519500 Rh2DG558700 Rh7AG105600 Rh7BG108400 Rh7CG110400 Rh7DG108700
rosa_wichuraiana	Rw2G044370 Rw7G009150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	1290
Acc65I	GGTACC	2 cut(s)	591, 944
AccB1I	GGYRCC	3 cut(s)	591, 944, 1165
AccBSI	CCGCTC	1 cut(s)	647
AciI	CCGC	8 cut(s)	414, 452, 523, 533, 598, 647, 731, 862
AclWI	GGATC	1 cut(s)	1209
AcoI	YGGCCR	2 cut(s)	183, 534
AcsI	RAATTY	3 cut(s)	359, 627, 1318
AcuI	CTGAAG	1 cut(s)	1310
AcvI	CACGTG	1 cut(s)	563
AfaI	GTAC	5 cut(s)	14, 440, 494, 593, 946
AfiI	CCNNNNNNNGG	5 cut(s)	528, 529, 532, 726, 1292
AflIII	ACRYGT	2 cut(s)	562, 1048
AgeI	ACCGGT	1 cut(s)	1072
AgsI	TTSAA	3 cut(s)	61, 632, 1229
AjnI	CCWGG	2 cut(s)	53, 1177
AjuI	GAANNNNNNNTTGG	2 cut(s)	1141, 1173
AluBI	AGCT	6 cut(s)	291, 506, 517, 556, 838, 1125
AluI	AGCT	6 cut(s)	291, 506, 517, 556, 838, 1125
Alw21I	GWGCWC	1 cut(s)	459
Alw26I	GTCTC	5 cut(s)	803, 875, 1202, 1247, 1265
AlwI	GGATC	1 cut(s)	1209
Ama87I	CYCGRG	1 cut(s)	1290
AoxI	GGCC	2 cut(s)	183, 534
ApeKI	GCWGC	1 cut(s)	983
ApoI	RAATTY	3 cut(s)	359, 627, 1318
AsiGI	ACCGGT	1 cut(s)	1072
Asp700I	GAANNNNTTC	1 cut(s)	107
Asp718I	GGTACC	2 cut(s)	591, 944
AspLEI	GCGC	2 cut(s)	973, 1383
AspS9I	GGNCC	1 cut(s)	1232
AsuC2I	CCSGG	4 cut(s)	476, 497, 510, 530
AsuHPI	GGTGA	1 cut(s)	1355
AvaI	CYCGRG	1 cut(s)	1290
AvaII	GGWCC	1 cut(s)	1232
BaeGI	GKGCMC	1 cut(s)	481
BanI	GGYRCC	3 cut(s)	591, 944, 1165
BauI	CACGAG	1 cut(s)	642
BbrPI	CACGTG	1 cut(s)	563
BbsI	GAAGAC	2 cut(s)	947, 1119
Bbv12I	GWGCWC	1 cut(s)	459
BbvCI	CCTCAGC	1 cut(s)	1140
BbvI	GCAGC	1 cut(s)	995
BccI	CCATC	2 cut(s)	917, 1012
BcgI	CGANNNNNNTGC	2 cut(s)	539, 573
BciT130I	CCWGG	2 cut(s)	55, 1179
BciVI	GTATCC	1 cut(s)	669
BclI	TGATCA	2 cut(s)	139, 199
BcnI	CCSGG	4 cut(s)	476, 497, 510, 530
BcoDI	GTCTC	5 cut(s)	803, 875, 1202, 1247, 1265
BfaI	CTAG	5 cut(s)	380, 788, 1079, 1205, 1365
BfmI	CTRYAG	2 cut(s)	901, 949
BfoI	RGCGCY	1 cut(s)	1384
BfuI	GTATCC	1 cut(s)	669
BisI	GCNGC	3 cut(s)	415, 534, 984
BlsI	GCNGC	3 cut(s)	416, 535, 985
Bme1390I	CCNGG	6 cut(s)	55, 476, 497, 510, 530, 1179
Bme18I	GGWCC	1 cut(s)	1232
BmeT110I	CYCGRG	1 cut(s)	1290
BmgT120I	GGNCC	1 cut(s)	1232
BmiI	GGNNCC	7 cut(s)	321, 593, 946, 1062, 1071, 1146, 1167
BmrFI	CCNGG	6 cut(s)	55, 476, 497, 510, 530, 1179
BmsI	GCATC	3 cut(s)	406, 439, 982
BpiI	GAAGAC	2 cut(s)	947, 1119
BplI	GAGNNNNNCTC	2 cut(s)	296, 328
BpmI	CTGGAG	1 cut(s)	1161
Bpu10I	CCTNAGC	1 cut(s)	1140
BpuEI	CTTGAG	3 cut(s)	59, 857, 863
BpuMI	CCSGG	4 cut(s)	476, 497, 510, 530
BsaAI	YACGTR	3 cut(s)	116, 442, 563
BsaJI	CCNNGG	2 cut(s)	174, 234
BsaWI	WCCGGW	2 cut(s)	588, 1072
BsaXI	ACNNNNNCTCC	2 cut(s)	734, 764
Bsc4I	CCNNNNNNNGG	5 cut(s)	528, 529, 532, 726, 1292
Bse118I	RCCGGY	1 cut(s)	1072
Bse1I	ACTGG	1 cut(s)	1169
Bse3DI	GCAATG	2 cut(s)	662, 1038
BseBI	CCWGG	2 cut(s)	55, 1179
BseDI	CCNNGG	2 cut(s)	174, 234
BseGI	GGATG	3 cut(s)	397, 823, 1023
BseLI	CCNNNNNNNGG	5 cut(s)	528, 529, 532, 726, 1292
BseMI	GCAATG	2 cut(s)	662, 1038
BseMII	CTCAG	2 cut(s)	818, 1131
BseNI	ACTGG	1 cut(s)	1169
BseRI	GAGGAG	4 cut(s)	356, 1073, 1275, 1278
BseSI	GKGCMC	1 cut(s)	481
BseXI	GCAGC	1 cut(s)	995
BshFI	GGCC	2 cut(s)	185, 536
BshNI	GGYRCC	3 cut(s)	591, 944, 1165
BshTI	ACCGGT	1 cut(s)	1072
BsiHKAI	GWGCWC	1 cut(s)	459
BsiHKCI	CYCGRG	1 cut(s)	1290
BsiSI	CCGG	8 cut(s)	475, 497, 509, 529, 574, 589, 865, 1073
BslI	CCNNNNNNNGG	5 cut(s)	528, 529, 532, 726, 1292
BsmAI	GTCTC	5 cut(s)	803, 875, 1202, 1247, 1265
BsmBI	CGTCTC	1 cut(s)	803
BsnI	GGCC	2 cut(s)	185, 536
BsoBI	CYCGRG	1 cut(s)	1290
Bsp1286I	GDGCHC	2 cut(s)	459, 481
Bsp143I	GATC	6 cut(s)	139, 199, 345, 622, 795, 1201
Bsp19I	CCATGG	2 cut(s)	174, 234
BspACI	CCGC	8 cut(s)	414, 452, 523, 533, 598, 647, 731, 862
BspANI	GGCC	2 cut(s)	185, 536
BspCNI	CTCAG	2 cut(s)	817, 1132
BspLI	GGNNCC	7 cut(s)	321, 593, 946, 1062, 1071, 1146, 1167
BspPI	GGATC	1 cut(s)	1209
BspT107I	GGYRCC	3 cut(s)	591, 944, 1165
BsrBI	CCGCTC	1 cut(s)	647
BsrDI	GCAATG	2 cut(s)	662, 1038
BsrFI	RCCGGY	1 cut(s)	1072
BsrI	ACTGG	1 cut(s)	1169
BssAI	RCCGGY	1 cut(s)	1072
BssECI	CCNNGG	2 cut(s)	174, 234
BssMI	GATC	6 cut(s)	139, 199, 345, 622, 795, 1201
BssSI	CACGAG	1 cut(s)	642
BssT1I	CCWWGG	2 cut(s)	174, 234
Bst2BI	CACGAG	1 cut(s)	642
Bst2UI	CCWGG	2 cut(s)	55, 1179
Bst4CI	ACNGT	3 cut(s)	137, 197, 265
Bst6I	CTCTTC	3 cut(s)	934, 1245, 1329
BstAPI	GCANNNNNTGC	1 cut(s)	992
BstBAI	YACGTR	3 cut(s)	116, 442, 563
BstC8I	GCNNGC	4 cut(s)	558, 785, 1137, 1311
BstDEI	CTNAG	3 cut(s)	804, 1140, 1413
BstDSI	CCRYGG	2 cut(s)	174, 234
BstENI	CCTNNNNNAGG	1 cut(s)	1290
BstF5I	GGATG	3 cut(s)	397, 823, 1023
BstH2I	RGCGCY	1 cut(s)	1384
BstHHI	GCGC	2 cut(s)	973, 1383
BstKTI	GATC	6 cut(s)	142, 202, 348, 625, 798, 1204
BstMAI	GTCTC	5 cut(s)	803, 875, 1202, 1247, 1265
BstMBI	GATC	6 cut(s)	139, 199, 345, 622, 795, 1201
BstMWI	GCNNNNNNNGC	3 cut(s)	566, 992, 1084
BstNI	CCWGG	2 cut(s)	55, 1179
BstNSI	RCATGY	3 cut(s)	773, 1052, 1313
BstSCI	CCNGG	6 cut(s)	53, 474, 495, 508, 528, 1177
BstSFI	CTRYAG	2 cut(s)	901, 949
BstSLI	GKGCMC	1 cut(s)	481
BstSNI	TACGTA	1 cut(s)	442
BstV1I	GCAGC	1 cut(s)	995
BstV2I	GAAGAC	2 cut(s)	947, 1119
BstX2I	RGATCY	1 cut(s)	1201
BstXI	CCANNNNNNTGG	1 cut(s)	181
BstYI	RGATCY	1 cut(s)	1201
BsuI	GTATCC	1 cut(s)	669
BsuRI	GGCC	2 cut(s)	185, 536
BtgI	CCRYGG	2 cut(s)	174, 234
BtgZI	GCGATG	1 cut(s)	462
BtsCI	GGATG	3 cut(s)	397, 823, 1023
BtsI	GCAGTG	1 cut(s)	285
BtsIMutI	CAGTG	3 cut(s)	142, 202, 285
Cac8I	GCNNGC	4 cut(s)	558, 785, 1137, 1311
CfoI	GCGC	2 cut(s)	973, 1383
Cfr10I	RCCGGY	1 cut(s)	1072
Cfr13I	GGNCC	1 cut(s)	1232
CseI	GACGC	2 cut(s)	695, 1124
Csp6I	GTAC	5 cut(s)	13, 439, 493, 592, 945
CspAI	ACCGGT	1 cut(s)	1072
CspCI	CAANNNNNGTGG	2 cut(s)	19, 54
CviAII	CATG	9 cut(s)	175, 235, 271, 739, 770, 888, 1049, 1096, 1310
CviQI	GTAC	5 cut(s)	13, 439, 493, 592, 945
DdeI	CTNAG	3 cut(s)	804, 1140, 1413
DpnI	GATC	6 cut(s)	141, 201, 347, 624, 797, 1203
DpnII	GATC	6 cut(s)	139, 199, 345, 622, 795, 1201
EaeI	YGGCCR	2 cut(s)	183, 534
Eam1104I	CTCTTC	3 cut(s)	934, 1245, 1329
EarI	CTCTTC	3 cut(s)	934, 1245, 1329
EciI	GGCGGA	1 cut(s)	587
Eco105I	TACGTA	1 cut(s)	442
Eco130I	CCWWGG	2 cut(s)	174, 234
Eco47I	GGWCC	1 cut(s)	1232
Eco57I	CTGAAG	1 cut(s)	1310
Eco72I	CACGTG	1 cut(s)	563
Eco88I	CYCGRG	1 cut(s)	1290
EcoNI	CCTNNNNNAGG	1 cut(s)	1290
EcoRII	CCWGG	2 cut(s)	53, 1177
EcoT14I	CCWWGG	2 cut(s)	174, 234
EcoT22I	ATGCAT	2 cut(s)	997, 1311
ErhI	CCWWGG	2 cut(s)	174, 234
Esp3I	CGTCTC	1 cut(s)	803
FaeI	CATG	9 cut(s)	178, 238, 274, 742, 773, 891, 1052, 1099, 1313
FalI	AAGNNNNNCTT	2 cut(s)	275, 307
FatI	CATG	9 cut(s)	174, 234, 270, 738, 769, 887, 1048, 1095, 1309
FauI	CCCGC	1 cut(s)	530
FauNDI	CATATG	1 cut(s)	1089
FbaI	TGATCA	2 cut(s)	139, 199
Fnu4HI	GCNGC	3 cut(s)	415, 534, 984
FokI	GGATG	3 cut(s)	384, 810, 1030
Fsp4HI	GCNGC	3 cut(s)	415, 534, 984
FspBI	CTAG	5 cut(s)	380, 788, 1079, 1205, 1365
GlaI	GCGC	2 cut(s)	972, 1382
GluI	GCNGC	3 cut(s)	415, 534, 984
GsuI	CTGGAG	1 cut(s)	1161
HaeII	RGCGCY	1 cut(s)	1384
HaeIII	GGCC	2 cut(s)	185, 536
HapII	CCGG	8 cut(s)	475, 497, 509, 529, 574, 589, 865, 1073
HgaI	GACGC	2 cut(s)	695, 1124
HhaI	GCGC	2 cut(s)	973, 1383
Hin1II	CATG	9 cut(s)	178, 238, 274, 742, 773, 891, 1052, 1099, 1313
Hin6I	GCGC	2 cut(s)	971, 1381
HinP1I	GCGC	2 cut(s)	971, 1381
HincII	GTYRAC	1 cut(s)	309
HindII	GTYRAC	1 cut(s)	309
HindIII	AAGCTT	2 cut(s)	289, 554
HinfI	GANTC	7 cut(s)	20, 305, 325, 716, 868, 884, 1359
HpaII	CCGG	8 cut(s)	475, 497, 509, 529, 574, 589, 865, 1073
HphI	GGTGA	1 cut(s)	1355
Hpy166II	GTNNAC	3 cut(s)	268, 309, 720
Hpy188I	TCNGA	7 cut(s)	19, 930, 960, 1069, 1236, 1299, 1388
Hpy8I	GTNNAC	3 cut(s)	268, 309, 720
Hpy99I	CGWCG	1 cut(s)	545
HpyAV	CCTTC	7 cut(s)	149, 209, 310, 889, 955, 1074, 1189
HpyCH4III	ACNGT	3 cut(s)	137, 197, 265
HpyCH4IV	ACGT	5 cut(s)	115, 441, 540, 562, 966
HpyCH4V	TGCA	6 cut(s)	560, 667, 704, 747, 995, 1309
HpyF10VI	GCNNNNNNNGC	3 cut(s)	566, 992, 1084
HpyF3I	CTNAG	3 cut(s)	804, 1140, 1413
HpySE526I	ACGT	5 cut(s)	115, 441, 540, 562, 966
Hsp92II	CATG	9 cut(s)	178, 238, 274, 742, 773, 891, 1052, 1099, 1313
HspAI	GCGC	2 cut(s)	971, 1381
KpnI	GGTACC	2 cut(s)	595, 948
Ksp22I	TGATCA	2 cut(s)	139, 199
Kzo9I	GATC	6 cut(s)	139, 199, 345, 622, 795, 1201
LmnI	GCTCC	5 cut(s)	319, 511, 694, 1060, 1132
Lsp1109I	GCAGC	1 cut(s)	995
LweI	GCATC	3 cut(s)	406, 439, 982
MaeI	CTAG	5 cut(s)	380, 788, 1079, 1205, 1365
MaeII	ACGT	5 cut(s)	115, 441, 540, 562, 966
MaeIII	GTNAC	5 cut(s)	121, 375, 1051, 1171, 1279
MalI	GATC	6 cut(s)	141, 201, 347, 624, 797, 1203
MbiI	CCGCTC	1 cut(s)	647
MboI	GATC	6 cut(s)	139, 199, 345, 622, 795, 1201
MboII	GAAGA	9 cut(s)	101, 164, 224, 947, 951, 1124, 1262, 1316, 1414
MflI	RGATCY	1 cut(s)	1201
MhlI	GDGCHC	2 cut(s)	459, 481
MluCI	AATT	5 cut(s)	162, 222, 359, 627, 1318
MlyI	GAGTC	5 cut(s)	314, 334, 725, 877, 1353
MmeI	TCCRAC	3 cut(s)	194, 254, 916
Mph1103I	ATGCAT	2 cut(s)	997, 1311
MroXI	GAANNNNTTC	1 cut(s)	107
MseI	TTAA	1 cut(s)	777
MslI	CAYNNNNRTG	4 cut(s)	135, 179, 195, 672
MspI	CCGG	8 cut(s)	475, 497, 509, 529, 574, 589, 865, 1073
MspR9I	CCNGG	6 cut(s)	55, 476, 497, 510, 530, 1179
MvaI	CCWGG	2 cut(s)	55, 1179
MwoI	GCNNNNNNNGC	3 cut(s)	566, 992, 1084
NciI	CCSGG	4 cut(s)	476, 497, 510, 530
NcoI	CCATGG	2 cut(s)	174, 234
NdeI	CATATG	1 cut(s)	1089
NdeII	GATC	6 cut(s)	139, 199, 345, 622, 795, 1201
NlaIII	CATG	9 cut(s)	178, 238, 274, 742, 773, 891, 1052, 1099, 1313
NlaIV	GGNNCC	7 cut(s)	321, 593, 946, 1062, 1071, 1146, 1167
NmeAIII	GCCGAG	2 cut(s)	211, 348
NmuCI	GTSAC	2 cut(s)	121, 1051
NsiI	ATGCAT	2 cut(s)	997, 1311
NspI	RCATGY	3 cut(s)	773, 1052, 1313
PaeI	GCATGC	1 cut(s)	1313
PaeR7I	CTCGAG	1 cut(s)	1290
PciI	ACATGT	1 cut(s)	1048
PcsI	WCGNNNNNNNCGW	2 cut(s)	320, 546
PdmI	GAANNNNTTC	1 cut(s)	107
PfeI	GAWTC	2 cut(s)	20, 884
PflFI	GACNNNGTC	1 cut(s)	304
PfoI	TCCNGGA	2 cut(s)	508, 1177
PinAI	ACCGGT	1 cut(s)	1072
PkrI	GCNGC	3 cut(s)	416, 535, 985
PleI	GAGTC	5 cut(s)	313, 333, 724, 876, 1353
PmaCI	CACGTG	1 cut(s)	563
PmlI	CACGTG	1 cut(s)	563
PpsI	GAGTC	5 cut(s)	313, 333, 724, 876, 1353
Ppu21I	YACGTR	3 cut(s)	116, 442, 563
PscI	ACATGT	1 cut(s)	1048
Psp6I	CCWGG	2 cut(s)	53, 1177
PspCI	CACGTG	1 cut(s)	563
PspGI	CCWGG	2 cut(s)	53, 1177
PspN4I	GGNNCC	7 cut(s)	321, 593, 946, 1062, 1071, 1146, 1167
PspPI	GGNCC	1 cut(s)	1232
PspXI	VCTCGAGB	1 cut(s)	1290
PsuI	RGATCY	1 cut(s)	1201
PsyI	GACNNNGTC	1 cut(s)	304
RsaI	GTAC	5 cut(s)	14, 440, 494, 593, 946
RsaNI	GTAC	5 cut(s)	13, 439, 493, 592, 945
RseI	CAYNNNNRTG	4 cut(s)	135, 179, 195, 672
SaqAI	TTAA	1 cut(s)	777
SatI	GCNGC	3 cut(s)	415, 534, 984
Sau3AI	GATC	6 cut(s)	139, 199, 345, 622, 795, 1201
Sau96I	GGNCC	1 cut(s)	1232
SchI	GAGTC	5 cut(s)	314, 334, 725, 877, 1353
ScrFI	CCNGG	6 cut(s)	55, 476, 497, 510, 530, 1179
SduI	GDGCHC	2 cut(s)	459, 481
SfaNI	GCATC	3 cut(s)	406, 439, 982
SfcI	CTRYAG	2 cut(s)	901, 949
Sfr274I	CTCGAG	1 cut(s)	1290
SinI	GGWCC	1 cut(s)	1232
SlaI	CTCGAG	1 cut(s)	1290
SmiMI	CAYNNNNRTG	4 cut(s)	135, 179, 195, 672
SmlI	CTYRAG	4 cut(s)	38, 842, 872, 1290
SmoI	CTYRAG	4 cut(s)	38, 842, 872, 1290
SnaBI	TACGTA	1 cut(s)	442
SphI	GCATGC	1 cut(s)	1313
Sse9I	AATT	5 cut(s)	162, 222, 359, 627, 1318
SsiI	CCGC	8 cut(s)	414, 452, 523, 533, 598, 647, 731, 862
SspMI	CTAG	5 cut(s)	380, 788, 1079, 1205, 1365
StyD4I	CCNGG	6 cut(s)	53, 474, 495, 508, 528, 1177
StyI	CCWWGG	2 cut(s)	174, 234
TaaI	ACNGT	3 cut(s)	137, 197, 265
TaiI	ACGT	5 cut(s)	118, 444, 543, 565, 969
TaqI	TCGA	3 cut(s)	314, 543, 1291
TaqII	GACCGA	1 cut(s)	317
TasI	AATT	5 cut(s)	162, 222, 359, 627, 1318
TauI	GCSGC	2 cut(s)	417, 536
TfiI	GAWTC	2 cut(s)	20, 884
Tru1I	TTAA	1 cut(s)	777
Tru9I	TTAA	1 cut(s)	777
TscAI	CASTG	3 cut(s)	142, 202, 285
TseFI	GTSAC	2 cut(s)	121, 1051
TseI	GCWGC	1 cut(s)	983
Tsp45I	GTSAC	2 cut(s)	121, 1051
TspGWI	ACGGA	2 cut(s)	1070, 1414
TspRI	CASTG	3 cut(s)	142, 202, 285
Tth111I	GACNNNGTC	1 cut(s)	304
VpaK11BI	GGWCC	1 cut(s)	1232
XagI	CCTNNNNNAGG	1 cut(s)	1290
XapI	RAATTY	3 cut(s)	359, 627, 1318
XbaI	TCTAGA	2 cut(s)	1204, 1364
XceI	RCATGY	3 cut(s)	773, 1052, 1313
XhoI	CTCGAG	1 cut(s)	1290
XmnI	GAANNNNTTC	1 cut(s)	107
XspI	CTAG	5 cut(s)	380, 788, 1079, 1205, 1365
Zsp2I	ATGCAT	2 cut(s)	997, 1311

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.