Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0011442

Physical Location & Seq

Genomic Coordinates Forward (+)

20305276 .. 20316970

Gene Structure 11695 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1177700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1920 bp

ATGGATGACGAAATTGATGCCTTTCACAAACAAAGAGATATAATTCCTCTTGATGTTAATGAAGAGGATGAAGATTCCGATGAAGACAACGAGCAACCCGTCTTTGGTTTGGAGAATATTCATGATGACGACGACGACAATGACGTTGATGATGAGCAGGATACCGGCTTTTCTGCAAAAATTGTTAGGCAGCAGAAGTTCTTAAGGGAGAAGTTTGGTGCTGAGGATGATTTGCATGATGATGAAGAAAACGAGGAAGAAGAAAAGCCTGTATGGGGTGGAAAGAAGCAAAACTATTATGGTGTTGATAATGGCGATTTTGAGCAAAAATCAAGTGATGATGAATCTGCTGAAGAGGAAGAAGCAGAAGTGGTGAGGCTGCAGAAAGAAAGGGCAAAATTTTCAACGCTGGATGACTATGGGCTTGACGATATTTGCGAAAATGAGAATAATAAGGAATTAACTTTTGAGGAAGTTTCAATGAAGGGAAACAGTACAAAAAGGTTTCCAATGGAAATTGATGTTGCAGATGGCACGGGTACAGCTTATGAGGAGGTGAAGAAAGATTTGAGTGCCTTGTCAAAGGAGGAGCAAATGGATGTTTTATATAATTCCACACCAGAATTAGTTGGGTTGTTGTCAGAGCTGAATGATGCACATGAAGAGCTTGAAAGAAAAGTTAATCCACTTCTGGGCAAGGTTAAAAAAGGGGAGGTCATGTTGGAAGGAGGGATGCGCTATTTGGAGATTAAGCAGCTTCTTCTGCTTTCCTATTGCCAAGCAATAACATTCTACCTTCTTCTCAAGTCTGAAGGACAACCAGTTCGTGACCATCCAGTACTTGCTCGCCTCATAGAGATCAAGATTTTGTTGGATAAGATGAAGCAACTTGATGGGAATCTACCATCTGATTTTGAGGAAATTTTAAACAAATATAATGGGGTGGAAGAGGTGGCAAAGTCTGGTAAAAAGACTTCTATTGACGCATCTGATTCTTTTGGTAAAGAATACAAACCCCAGCCTATCTTGGTTGAGGAAGAAGAAGCAACTGTGTTGCATGACATTGCTAAGGTAGAAATAGTGGAGCCTCTGAAGGATAATGAAAACAAGGTGGGAAAACACAAATTTCAGAATGATGAAGTTGGTGTACAGAGCATGAAAATGTTGAAAGTAAGAGCTGCCCTTGAGGAAAAGTTGAAGCAGAAGGGTGTCTTCAGTTCCATCGCTCTGAAAACTGATAAACCCAGGAAACTTCTGAAGCCATTGAATGGGAAGCTTGAATCATATGATGATTTTGATGATGACACTGTAAATATTGAGGGTGGCGTCCATGGATTGAACAATGGTCGTGCAAGTAAACTCTCCCAACTTGCTGCTAATCCAAATAAGTCCAAGAAGGCTATCTCAGGTGATGATGATTTACCTCGGCGAGATGATATTGGGGAAAGGCGGAGAAAACATGAGCTTCGAGTGCTTGCAGGAGCTGGAATCAAGTCTCAGGATGACGCTGGAGATGAAGATGGCACTATTTCGGATGATGGAGATGTGGAGATGGAGGATAGTGAAACTGGAGATTCAGAAGATGACTTCTACGAACAAGTGAAAGAAAAACGAGCTGCAAAACTTGCTGCCAAAGCTGAGATTTATTCGAGGTCCTCGATGGTTCCATCTTTGGTGGAAACTGAAACTGTTGATGGGAAACGCCATATTACTTATCAGATGGAGAAGAACAGGGGACTTACTCGTGCTCGTAAGAAGCTGATCAAGAATCCAAGAAAGAAGTACAAGTTGAAGCACCAGAAGGCAGTGAAGAGTAGAAAAGGACAGGTAAGAGAGGTGAGGAAGCCTTTAGGTCCGTATGGTGGAGAGGCCACCGGAATCAATGCAGGAATCAGTCGGAGCACCAGATTCAAGAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000462 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0022613 GO:0030490 GO:0030684 GO:0031974 GO:0031981 GO:0032040 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sas10 Sas10_Utp3

Protein Analysis

640

Amino Acids

72.23

Weight (kDa)

4.83

Isoelectric Point (pI)

42.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sas10_Utp3	PF04000	213 - 293	2.8e-19	Sas10/Utp3/C1D family
Sas10	PF09368	565 - 637	4.7e-27	Sas10 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011442)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43650
fragaria_vesca	FvH4_5g27692 FvH4_5g27692 FvH4_5g27692
malus_domestica	MD04G1048900.v1.1 MD17G1177700.v1.1
prunus_persica	Prupe.1G206800_v2.0.a1
pyrus_communis	pycom04g04300
rosa_chinensis	RchiOBHm_Chr7g0219421
rosa_laevigata	RLG00000002290
rosa_multiflora	Rmu_sc0002460.1_g000021 Rmu_sc0003767.1_g000014
rosa_roxburghii	Rroxscaffold_3G00240050
rosa_rugosa	Rorug07G0184900
rosa_samantha	Rh7AG327800 Rh7BG317800 Rh7CG345200 Rh7DG323200
rosa_wichuraiana	Rw7G027650 Rw7G027690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1268
AciI	CCGC	1 cut(s)	1450
AcsI	RAATTY	3 cut(s)	398, 921, 1124
AcuI	CTGAAG	5 cut(s)	372, 831, 1112, 1198, 1277
AcyI	GRCGYC	1 cut(s)	1326
AfaI	GTAC	5 cut(s)	496, 541, 840, 1149, 1784
AfiI	CCNNNNNNNGG	5 cut(s)	104, 275, 692, 1268, 1862
AflII	CTTAAG	1 cut(s)	202
AjnI	CCWGG	1 cut(s)	1244
AloI	GAACNNNNNNTCC	2 cut(s)	807, 839
Alw21I	GWGCWC	2 cut(s)	1750, 1904
Alw26I	GTCTC	1 cut(s)	1500
AlwNI	CAGNNNCTG	1 cut(s)	1484
AoxI	GGCC	1 cut(s)	1869
ApeKI	GCWGC	7 cut(s)	190, 379, 754, 1178, 1373, 1616, 1628
ApoI	RAATTY	3 cut(s)	398, 921, 1124
ArsI	GACNNNNNNTTYG	2 cut(s)	807, 839
AspLEI	GCGC	1 cut(s)	738
AspS9I	GGNCC	2 cut(s)	1653, 1853
AsuHPI	GGTGA	4 cut(s)	385, 568, 1421, 1849
AvaII	GGWCC	2 cut(s)	1653, 1853
BarI	GAAGNNNNNNTAC	4 cut(s)	958, 990, 1131, 1163
BauI	CACGAG	1 cut(s)	1743
BbsI	GAAGAC	2 cut(s)	90, 1204
Bbv12I	GWGCWC	2 cut(s)	1750, 1904
BbvCI	CCTCAGC	1 cut(s)	222
BbvI	GCAGC	7 cut(s)	202, 366, 766, 1165, 1360, 1603, 1615
BcgI	CGANNNNNNTGC	1 cut(s)	33
BciT130I	CCWGG	1 cut(s)	1246
BciVI	GTATCC	1 cut(s)	154
BclI	TGATCA	1 cut(s)	1761
BcoDI	GTCTC	1 cut(s)	1500
BfmI	CTRYAG	1 cut(s)	380
BfrI	CTTAAG	1 cut(s)	202
BfuI	GTATCC	1 cut(s)	154
BisI	GCNGC	7 cut(s)	191, 380, 755, 1179, 1374, 1617, 1629
BlsI	GCNGC	7 cut(s)	192, 381, 756, 1180, 1375, 1618, 1630
BmcAI	AGTACT	1 cut(s)	840
Bme1390I	CCNGG	1 cut(s)	1246
Bme18I	GGWCC	2 cut(s)	1653, 1853
BmgT120I	GGNCC	2 cut(s)	1653, 1853
BmiI	GGNNCC	2 cut(s)	1086, 1665
BmrFI	CCNGG	1 cut(s)	1246
BmsI	GCATC	4 cut(s)	7, 643, 723, 995
BpiI	GAAGAC	2 cut(s)	90, 1204
BpmI	CTGGAG	2 cut(s)	1530, 1590
Bpu10I	CCTNAGC	2 cut(s)	222, 1068
BpuEI	CTTGAG	2 cut(s)	788, 1205
BsaBI	GATNNNNATC	1 cut(s)	897
BsaHI	GRCGYC	1 cut(s)	1326
BsaJI	CCNNGG	3 cut(s)	1244, 1330, 1424
BsaWI	WCCGGW	1 cut(s)	1874
BsaXI	ACNNNNNCTCC	2 cut(s)	704, 734
Bsc4I	CCNNNNNNNGG	5 cut(s)	104, 275, 692, 1268, 1862
Bse118I	RCCGGY	1 cut(s)	164
Bse1I	ACTGG	3 cut(s)	821, 836, 1573
Bse3DI	GCAATG	1 cut(s)	1062
Bse8I	GATNNNNATC	1 cut(s)	897
BseBI	CCWGG	1 cut(s)	1246
BseDI	CCNNGG	3 cut(s)	1244, 1330, 1424
BseGI	GGATG	9 cut(s)	10, 73, 232, 418, 604, 738, 832, 1507, 1540
BseJI	GATNNNNATC	1 cut(s)	897
BseLI	CCNNNNNNNGG	5 cut(s)	104, 275, 692, 1268, 1862
BseMI	GCAATG	1 cut(s)	1062
BseMII	CTCAG	4 cut(s)	213, 1419, 1511, 1629
BseNI	ACTGG	3 cut(s)	821, 836, 1573
BseRI	GAGGAG	2 cut(s)	566, 602
BseXI	GCAGC	7 cut(s)	202, 366, 766, 1165, 1360, 1603, 1615
BseYI	CCCAGC	1 cut(s)	1017
BshFI	GGCC	1 cut(s)	1871
BsiHKAI	GWGCWC	2 cut(s)	1750, 1904
BsiSI	CCGG	2 cut(s)	165, 1875
BslFI	GGGAC	1 cut(s)	1749
BslI	CCNNNNNNNGG	5 cut(s)	104, 275, 692, 1268, 1862
BsmAI	GTCTC	1 cut(s)	1500
BsmFI	GGGAC	1 cut(s)	1749
BsnI	GGCC	1 cut(s)	1871
Bsp1286I	GDGCHC	2 cut(s)	1750, 1904
Bsp1407I	TGTACA	1 cut(s)	1147
Bsp143I	GATC	2 cut(s)	858, 1761
Bsp19I	CCATGG	1 cut(s)	1330
BspACI	CCGC	1 cut(s)	1450
BspANI	GGCC	1 cut(s)	1871
BspCNI	CTCAG	4 cut(s)	214, 1418, 1510, 1630
BspHI	TCATGA	1 cut(s)	121
BspLI	GGNNCC	2 cut(s)	1086, 1665
BspMAI	CTGCAG	1 cut(s)	384
BspQI	GCTCTTC	1 cut(s)	657
BspTI	CTTAAG	1 cut(s)	202
BsrDI	GCAATG	1 cut(s)	1062
BsrFI	RCCGGY	1 cut(s)	164
BsrGI	TGTACA	1 cut(s)	1147
BsrI	ACTGG	3 cut(s)	821, 836, 1573
BssAI	RCCGGY	1 cut(s)	164
BssECI	CCNNGG	3 cut(s)	1244, 1330, 1424
BssMI	GATC	2 cut(s)	858, 1761
BssNI	GRCGYC	1 cut(s)	1326
BssSI	CACGAG	1 cut(s)	1743
BssT1I	CCWWGG	1 cut(s)	1330
Bst2BI	CACGAG	1 cut(s)	1743
Bst2UI	CCWGG	1 cut(s)	1246
Bst4CI	ACNGT	4 cut(s)	494, 1051, 1309, 1690
Bst6I	CTCTTC	5 cut(s)	57, 348, 657, 942, 1805
BstACI	GRCGYC	1 cut(s)	1326
BstAFI	CTTAAG	1 cut(s)	202
BstAPI	GCANNNNNTGC	1 cut(s)	1625
BstAUI	TGTACA	1 cut(s)	1147
BstC8I	GCNNGC	2 cut(s)	847, 1476
BstDEI	CTNAG	5 cut(s)	222, 1068, 1405, 1497, 1638
BstDSI	CCRYGG	1 cut(s)	1330
BstF5I	GGATG	9 cut(s)	10, 73, 232, 418, 604, 738, 832, 1507, 1540
BstHHI	GCGC	1 cut(s)	738
BstKTI	GATC	2 cut(s)	861, 1764
BstMAI	GTCTC	1 cut(s)	1500
BstMBI	GATC	2 cut(s)	858, 1761
BstMWI	GCNNNNNNNGC	4 cut(s)	763, 1471, 1625, 1634
BstNI	CCWGG	1 cut(s)	1246
BstSCI	CCNGG	1 cut(s)	1244
BstSFI	CTRYAG	1 cut(s)	380
BstV1I	GCAGC	7 cut(s)	202, 366, 766, 1165, 1360, 1603, 1615
BstV2I	GAAGAC	2 cut(s)	90, 1204
BsuI	GTATCC	1 cut(s)	154
BsuRI	GGCC	1 cut(s)	1871
BtgI	CCRYGG	1 cut(s)	1330
BtgZI	GCGATG	1 cut(s)	1207
BtsCI	GGATG	9 cut(s)	10, 73, 232, 418, 604, 738, 832, 1507, 1540
BtsI	GCAGTG	1 cut(s)	1812
BtsIMutI	CAGTG	2 cut(s)	1305, 1812
Cac8I	GCNNGC	2 cut(s)	847, 1476
CaiI	CAGNNNCTG	1 cut(s)	1484
CciI	TCATGA	1 cut(s)	121
CfoI	GCGC	1 cut(s)	738
Cfr10I	RCCGGY	1 cut(s)	164
Cfr13I	GGNCC	2 cut(s)	1653, 1853
CseI	GACGC	3 cut(s)	992, 1315, 1514
Csp6I	GTAC	5 cut(s)	495, 540, 839, 1148, 1783
CviAII	CATG	8 cut(s)	122, 236, 659, 718, 1058, 1156, 1331, 1460
CviQI	GTAC	5 cut(s)	495, 540, 839, 1148, 1783
DdeI	CTNAG	5 cut(s)	222, 1068, 1405, 1497, 1638
DpnI	GATC	2 cut(s)	860, 1763
DpnII	GATC	2 cut(s)	858, 1761
DraI	TTTAAA	1 cut(s)	927
Eam1104I	CTCTTC	5 cut(s)	57, 348, 657, 942, 1805
EarI	CTCTTC	5 cut(s)	57, 348, 657, 942, 1805
EciI	GGCGGA	1 cut(s)	1465
Eco130I	CCWWGG	1 cut(s)	1330
Eco47I	GGWCC	2 cut(s)	1653, 1853
Eco57I	CTGAAG	5 cut(s)	372, 831, 1112, 1198, 1277
EcoO109I	RGGNCCY	1 cut(s)	1653
EcoRII	CCWGG	1 cut(s)	1244
EcoT14I	CCWWGG	1 cut(s)	1330
ErhI	CCWWGG	1 cut(s)	1330
FaeI	CATG	8 cut(s)	125, 239, 662, 721, 1061, 1159, 1334, 1463
FalI	AAGNNNNNCTT	2 cut(s)	1196, 1228
FaqI	GGGAC	1 cut(s)	1749
FatI	CATG	8 cut(s)	121, 235, 658, 717, 1057, 1155, 1330, 1459
FauNDI	CATATG	1 cut(s)	1285
FbaI	TGATCA	1 cut(s)	1761
Fnu4HI	GCNGC	7 cut(s)	191, 380, 755, 1179, 1374, 1617, 1629
FokI	GGATG	9 cut(s)	17, 80, 239, 425, 611, 745, 819, 1514, 1547
Fsp4HI	GCNGC	7 cut(s)	191, 380, 755, 1179, 1374, 1617, 1629
GlaI	GCGC	1 cut(s)	737
GluI	GCNGC	7 cut(s)	191, 380, 755, 1179, 1374, 1617, 1629
GsaI	CCCAGC	1 cut(s)	1021
GsuI	CTGGAG	2 cut(s)	1530, 1590
HaeIII	GGCC	1 cut(s)	1871
HapII	CCGG	2 cut(s)	165, 1875
HgaI	GACGC	3 cut(s)	992, 1315, 1514
HhaI	GCGC	1 cut(s)	738
Hin1I	GRCGYC	1 cut(s)	1326
Hin1II	CATG	8 cut(s)	125, 239, 662, 721, 1061, 1159, 1334, 1463
Hin6I	GCGC	1 cut(s)	736
HinP1I	GCGC	1 cut(s)	736
HindIII	AAGCTT	1 cut(s)	1274
HpaII	CCGG	2 cut(s)	165, 1875
HphI	GGTGA	4 cut(s)	385, 568, 1421, 1849
Hpy166II	GTNNAC	2 cut(s)	1148, 1358
Hpy188III	TCNNGA	7 cut(s)	50, 122, 827, 862, 1499, 1765, 1912
Hpy8I	GTNNAC	2 cut(s)	1148, 1358
Hpy99I	CGWCG	2 cut(s)	134, 137
HpyAV	CCTTC	8 cut(s)	478, 719, 806, 806, 1087, 1198, 1390, 1795
HpyCH4III	ACNGT	4 cut(s)	494, 1051, 1309, 1690
HpyCH4IV	ACGT	1 cut(s)	144
HpyF10VI	GCNNNNNNNGC	4 cut(s)	763, 1471, 1625, 1634
HpyF3I	CTNAG	5 cut(s)	222, 1068, 1405, 1497, 1638
HpySE526I	ACGT	1 cut(s)	144
Hsp92I	GRCGYC	1 cut(s)	1326
Hsp92II	CATG	8 cut(s)	125, 239, 662, 721, 1061, 1159, 1334, 1463
HspAI	GCGC	1 cut(s)	736
Ksp22I	TGATCA	1 cut(s)	1761
Kzo9I	GATC	2 cut(s)	858, 1761
LguI	GCTCTTC	1 cut(s)	657
LmnI	GCTCC	4 cut(s)	589, 1084, 1481, 1899
Lsp1109I	GCAGC	7 cut(s)	202, 366, 766, 1165, 1360, 1603, 1615
LweI	GCATC	4 cut(s)	7, 643, 723, 995
MaeII	ACGT	1 cut(s)	144
MaeIII	GTNAC	1 cut(s)	827
MalI	GATC	2 cut(s)	860, 1763
MboI	GATC	2 cut(s)	858, 1761
MhlI	GDGCHC	2 cut(s)	1750, 1904
MmeI	TCCRAC	3 cut(s)	702, 852, 1877
MseI	TTAA	7 cut(s)	57, 203, 461, 681, 702, 750, 926
MslI	CAYNNNNRTG	2 cut(s)	240, 1160
MspCI	CTTAAG	1 cut(s)	202
MspI	CCGG	2 cut(s)	165, 1875
MspR9I	CCNGG	1 cut(s)	1246
MvaI	CCWGG	1 cut(s)	1246
MwoI	GCNNNNNNNGC	4 cut(s)	763, 1471, 1625, 1634
NcoI	CCATGG	1 cut(s)	1330
NdeI	CATATG	1 cut(s)	1285
NdeII	GATC	2 cut(s)	858, 1761
NlaIII	CATG	8 cut(s)	125, 239, 662, 721, 1061, 1159, 1334, 1463
NlaIV	GGNNCC	2 cut(s)	1086, 1665
NmeAIII	GCCGAG	1 cut(s)	1405
NmuCI	GTSAC	1 cut(s)	827
PagI	TCATGA	1 cut(s)	121
PciSI	GCTCTTC	1 cut(s)	657
PcsI	WCGNNNNNNNCGW	4 cut(s)	96, 141, 435, 1655
PflMI	CCANNNNNTGG	1 cut(s)	1268
PkrI	GCNGC	7 cut(s)	192, 381, 756, 1180, 1375, 1618, 1630
PpuMI	RGGWCCY	1 cut(s)	1653
Psp5II	RGGWCCY	1 cut(s)	1653
Psp6I	CCWGG	1 cut(s)	1244
PspFI	CCCAGC	1 cut(s)	1017
PspGI	CCWGG	1 cut(s)	1244
PspN4I	GGNNCC	2 cut(s)	1086, 1665
PspPI	GGNCC	2 cut(s)	1653, 1853
PspPPI	RGGWCCY	1 cut(s)	1653
PstI	CTGCAG	1 cut(s)	384
PstNI	CAGNNNCTG	1 cut(s)	1484
RsaI	GTAC	5 cut(s)	496, 541, 840, 1149, 1784
RsaNI	GTAC	5 cut(s)	495, 540, 839, 1148, 1783
RseI	CAYNNNNRTG	2 cut(s)	240, 1160
SapI	GCTCTTC	1 cut(s)	657
SaqAI	TTAA	7 cut(s)	57, 203, 461, 681, 702, 750, 926
SatI	GCNGC	7 cut(s)	191, 380, 755, 1179, 1374, 1617, 1629
Sau3AI	GATC	2 cut(s)	858, 1761
Sau96I	GGNCC	2 cut(s)	1653, 1853
ScaI	AGTACT	1 cut(s)	840
ScrFI	CCNGG	1 cut(s)	1246
SduI	GDGCHC	2 cut(s)	1750, 1904
SfaNI	GCATC	4 cut(s)	7, 643, 723, 995
SfcI	CTRYAG	1 cut(s)	380
SinI	GGWCC	2 cut(s)	1653, 1853
SmiMI	CAYNNNNRTG	2 cut(s)	240, 1160
SmlI	CTYRAG	3 cut(s)	202, 803, 1184
SmoI	CTYRAG	3 cut(s)	202, 803, 1184
SsiI	CCGC	1 cut(s)	1450
SspI	AATATT	2 cut(s)	118, 1315
StyD4I	CCNGG	1 cut(s)	1244
StyI	CCWWGG	1 cut(s)	1330
TaaI	ACNGT	4 cut(s)	494, 1051, 1309, 1690
TaiI	ACGT	1 cut(s)	147
TaqI	TCGA	3 cut(s)	1468, 1649, 1658
TatI	WGTACW	4 cut(s)	494, 838, 1147, 1782
Tru1I	TTAA	7 cut(s)	57, 203, 461, 681, 702, 750, 926
Tru9I	TTAA	7 cut(s)	57, 203, 461, 681, 702, 750, 926
TscAI	CASTG	2 cut(s)	1312, 1812
TseFI	GTSAC	1 cut(s)	827
TseI	GCWGC	7 cut(s)	190, 379, 754, 1178, 1373, 1616, 1628
Tsp45I	GTSAC	1 cut(s)	827
TspGWI	ACGGA	1 cut(s)	1845
TspRI	CASTG	2 cut(s)	1312, 1812
Van91I	CCANNNNNTGG	1 cut(s)	1268
Vha464I	CTTAAG	1 cut(s)	202
VpaK11BI	GGWCC	2 cut(s)	1653, 1853
XapI	RAATTY	3 cut(s)	398, 921, 1124
ZrmI	AGTACT	1 cut(s)	840

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.