MD17G1212200.v1.1

Phosphatidylcholine diacylglycerol cholinephosphotransferase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014419
Physical Location & Seq
Reverse (-)
25928809 .. 25930797
1989 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1212200.v1.1.491

Sequence Viewer

Length: 828 bp
ATGAACGGCGCCGTTTCATCCCTCAAGCCTAGGCGCAAAAGGGTCCCAAACGGCAAGCCCATTCGCGTCGTTTCCCCTTCGCCCGCAGACCACAAGGAGGAGGCGATGTATTCTTCTTCCAAGGCCAAGAAGATCAACCTCAACGGCGGTGGCAAGGCCTCCTTCATGACGTGGAGGCTGCATGACGCCGTCCACGTGGCCAAACACCATTGGATACCCTGCCTCTTCGCAATGGGGGTCCTCTTCTTCATGGGCGTGGAGTACACGCTCCGGATGGTGCCGTCGTCGTCGCCGCCGTTCGATATCGGGTTCGTCACCACCCGCTCCCTCCACCTCCTCCTGGCTTCCAAGCCCAAGCTTAACACCTTGCTCGCTGGTCTTAACACGGTGTTTGTGGCAATGCAGACCACGTATATACTATGGGCATGGCTAGTAGAGGGCCGCCCAAGACCCACAATCGCGGCCGTCTTCATGTTCACGTGCCGGGGCATCCTCGGCTACGCCACCCAACTTCCATTGCCTCAGGGGTTTTTGGGGTCAGGAGCGGACTTCCCAGTCGGGAATGTGTCGTTTTTCCTCTTCTTCTCGGGGCATGTAGCCGGGTCTGTGATTGCATCCTTGGATATGAGAAGAATGCAGAGGTGGGAAATGGCGTGGACATTCGATGCACTCAATGTCCTGCAATCGGTGAGACTGCTGGGGACAAGAGGGCACTACACGATAGACTTGGCCGTGGGGGTTGGAGCTGGTTTTCTGTTTGATTCTCTGGCCGGAAAGTACGAGGAAAGCAAGAGATCAACTTCTACCAAAGAGGCTCTGTTTCCTTGA

Protein Analysis

276

Amino Acids

30.3

Weight (kDa)

10.08

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AtPDCT1_2 PF24788 100 - 259 9.1e-92 AtPDCT1/2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014419)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15820
fragaria_vesca FvH4_6g29670
malus_domestica MD17G1212200.v1.1
prunus_persica Prupe.3G087400_v2.0.a1 Prupe.3G087500_v2.0.a1
pyrus_communis pycom17g21710
rosa_chinensis RchiOBHm_Chr2g0135261
rosa_laevigata RLG00000019458
rosa_multiflora Rmu_sc0003835.1_g000021 Rmu_ssc0000187.1_g000016
rosa_roxburghii Rroxscaffold_2G00109330
rosa_rugosa Rorug02G0324400
rosa_samantha Rh2AG370700 Rh2BG380900 Rh2CG360200 Rh2DG397400
rosa_wichuraiana Rw2G030630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 554
AccB1I GGYRCC 2 cut(s) 8, 277
AccBSI CCGCTC 2 cut(s) 324, 545
AccII CGCG 2 cut(s) 66, 461
AccIII TCCGGA 1 cut(s) 270
AciI CCGC 7 cut(s) 84, 147, 293, 322, 442, 461, 545
AcoI YGGCCR 4 cut(s) 198, 462, 729, 768
AcvI CACGTG 2 cut(s) 196, 480
AcyI GRCGYC 2 cut(s) 9, 186
AfaI GTAC 2 cut(s) 263, 779
AfiI CCNNNNNNNGG 3 cut(s) 97, 340, 685
AjiI CACGTC 1 cut(s) 171
AjnI CCWGG 1 cut(s) 339
AluBI AGCT 2 cut(s) 358, 746
AluI AGCT 2 cut(s) 358, 746
Alw26I GTCTC 1 cut(s) 685
Ama87I CYCGRG 1 cut(s) 586
Aor13HI TCCGGA 1 cut(s) 270
AoxI GGCC 7 cut(s) 123, 156, 198, 439, 462, 729, 768
ApeKI GCWGC 1 cut(s) 178
AspA2I CCTAGG 1 cut(s) 29
AspLEI GCGC 2 cut(s) 11, 36
AspS9I GGNCC 3 cut(s) 43, 238, 439
AsuC2I CCSGG 2 cut(s) 485, 601
AsuHPI GGTGA 2 cut(s) 307, 700
AvaI CYCGRG 1 cut(s) 586
AvaII GGWCC 2 cut(s) 43, 238
AvrII CCTAGG 1 cut(s) 29
AxyI CCTNAGG 1 cut(s) 522
BaeGI GKGCMC 1 cut(s) 714
BalI TGGCCA 1 cut(s) 200
BanI GGYRCC 2 cut(s) 8, 277
BbrPI CACGTG 2 cut(s) 196, 480
BbsI GAAGAC 1 cut(s) 460
BbvI GCAGC 1 cut(s) 165
BccI CCATC 1 cut(s) 268
BceAI ACGGC 8 cut(s) 22, 67, 160, 173, 265, 280, 449, 716
BciT130I CCWGG 1 cut(s) 341
BciVI GTATCC 1 cut(s) 207
BcnI CCSGG 2 cut(s) 485, 601
BcoDI GTCTC 1 cut(s) 685
BfaI CTAG 2 cut(s) 30, 431
BfoI RGCGCY 1 cut(s) 12
BfuI GTATCC 1 cut(s) 207
BisI GCNGC 4 cut(s) 179, 293, 442, 462
BlnI CCTAGG 1 cut(s) 29
BlsI GCNGC 4 cut(s) 180, 294, 443, 463
Bme1390I CCNGG 3 cut(s) 341, 485, 601
Bme18I GGWCC 2 cut(s) 43, 238
BmeT110I CYCGRG 1 cut(s) 586
BmgBI CACGTC 1 cut(s) 171
BmgT120I GGNCC 3 cut(s) 43, 238, 439
BmiI GGNNCC 5 cut(s) 10, 44, 45, 239, 279
BmrFI CCNGG 3 cut(s) 341, 485, 601
BmrI ACTGGG 1 cut(s) 548
BmsI GCATC 3 cut(s) 498, 623, 655
BmuI ACTGGG 1 cut(s) 548
BpiI GAAGAC 1 cut(s) 460
BpuEI CTTGAG 1 cut(s) 8
BpuMI CCSGG 2 cut(s) 485, 601
BsaAI YACGTR 3 cut(s) 196, 411, 480
BsaHI GRCGYC 2 cut(s) 9, 186
BsaJI CCNNGG 6 cut(s) 29, 120, 484, 493, 618, 732
BsaWI WCCGGW 1 cut(s) 270
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 340, 685
Bse1I ACTGG 1 cut(s) 554
Bse21I CCTNAGG 1 cut(s) 522
Bse3DI GCAATG 3 cut(s) 237, 405, 515
BseAI TCCGGA 1 cut(s) 270
BseBI CCWGG 1 cut(s) 341
BseDI CCNNGG 6 cut(s) 29, 120, 484, 493, 618, 732
BseGI GGATG 4 cut(s) 17, 279, 489, 614
BseLI CCNNNNNNNGG 3 cut(s) 97, 340, 685
BseMI GCAATG 3 cut(s) 237, 405, 515
BseMII CTCAG 1 cut(s) 536
BseNI ACTGG 1 cut(s) 554
BseRI GAGGAG 2 cut(s) 113, 326
BseSI GKGCMC 1 cut(s) 714
BseX3I CGGCCG 1 cut(s) 462
BseXI GCAGC 1 cut(s) 165
BseYI CCCAGC 1 cut(s) 697
Bsh1236I CGCG 2 cut(s) 66, 461
Bsh1285I CGRYCG 1 cut(s) 465
BshFI GGCC 7 cut(s) 125, 158, 200, 441, 464, 731, 770
BshNI GGYRCC 2 cut(s) 8, 277
BsiEI CGRYCG 1 cut(s) 465
BsiHKCI CYCGRG 1 cut(s) 586
BsiSI CCGG 4 cut(s) 271, 484, 600, 771
BslFI GGGAC 2 cut(s) 29, 715
BslI CCNNNNNNNGG 3 cut(s) 97, 340, 685
BsmAI GTCTC 1 cut(s) 685
BsmFI GGGAC 2 cut(s) 29, 715
BsmI GAATGC 1 cut(s) 639
BsnI GGCC 7 cut(s) 125, 158, 200, 441, 464, 731, 770
BsoBI CYCGRG 1 cut(s) 586
Bsp1286I GDGCHC 1 cut(s) 714
Bsp13I TCCGGA 1 cut(s) 270
Bsp143I GATC 2 cut(s) 132, 794
BspACI CCGC 7 cut(s) 84, 147, 293, 322, 442, 461, 545
BspANI GGCC 7 cut(s) 125, 158, 200, 441, 464, 731, 770
BspCNI CTCAG 1 cut(s) 535
BspEI TCCGGA 1 cut(s) 270
BspFNI CGCG 2 cut(s) 66, 461
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 5 cut(s) 10, 44, 45, 239, 279
BspT107I GGYRCC 2 cut(s) 8, 277
BsrBI CCGCTC 2 cut(s) 324, 545
BsrDI GCAATG 3 cut(s) 237, 405, 515
BsrI ACTGG 1 cut(s) 554
BssECI CCNNGG 6 cut(s) 29, 120, 484, 493, 618, 732
BssMI GATC 2 cut(s) 132, 794
BssNI GRCGYC 2 cut(s) 9, 186
BssT1I CCWWGG 3 cut(s) 29, 120, 618
Bst2UI CCWGG 1 cut(s) 341
Bst4CI ACNGT 1 cut(s) 388
Bst6I CTCTTC 3 cut(s) 230, 248, 584
BstACI GRCGYC 2 cut(s) 9, 186
BstBAI YACGTR 3 cut(s) 196, 411, 480
BstC8I GCNNGC 3 cut(s) 56, 84, 372
BstDEI CTNAG 1 cut(s) 522
BstDSI CCRYGG 1 cut(s) 732
BstF5I GGATG 4 cut(s) 17, 279, 489, 614
BstFNI CGCG 2 cut(s) 66, 461
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 2 cut(s) 11, 36
BstKTI GATC 2 cut(s) 135, 797
BstMAI GTCTC 1 cut(s) 685
BstMBI GATC 2 cut(s) 132, 794
BstMCI CGRYCG 1 cut(s) 465
BstMWI GCNNNNNNNGC 1 cut(s) 495
BstNI CCWGG 1 cut(s) 341
BstNSI RCATGY 1 cut(s) 596
BstSCI CCNGG 3 cut(s) 339, 483, 599
BstSLI GKGCMC 1 cut(s) 714
BstUI CGCG 2 cut(s) 66, 461
BstV1I GCAGC 1 cut(s) 165
BstV2I GAAGAC 1 cut(s) 460
BstZI CGGCCG 1 cut(s) 462
Bsu36I CCTNAGG 1 cut(s) 522
BsuI GTATCC 1 cut(s) 207
BsuRI GGCC 7 cut(s) 125, 158, 200, 441, 464, 731, 770
BtgI CCRYGG 1 cut(s) 732
BtgZI GCGATG 1 cut(s) 119
BtrI CACGTC 1 cut(s) 171
BtsCI GGATG 4 cut(s) 17, 279, 489, 614
Cac8I GCNNGC 3 cut(s) 56, 84, 372
CciI TCATGA 1 cut(s) 165
CfoI GCGC 2 cut(s) 11, 36
Cfr13I GGNCC 3 cut(s) 43, 238, 439
CseI GACGC 2 cut(s) 55, 194
Csp6I GTAC 2 cut(s) 262, 778
CspCI CAANNNNNGTGG 2 cut(s) 130, 165
CviAII CATG 6 cut(s) 166, 182, 250, 426, 472, 593
CviQI GTAC 2 cut(s) 262, 778
DdeI CTNAG 1 cut(s) 522
DinI GGCGCC 1 cut(s) 10
DpnI GATC 2 cut(s) 134, 796
DpnII GATC 2 cut(s) 132, 794
DrdI GACNNNNNNGTC 1 cut(s) 554
DseDI GACNNNNNNGTC 1 cut(s) 554
EaeI YGGCCR 4 cut(s) 198, 462, 729, 768
EagI CGGCCG 1 cut(s) 462
Eam1104I CTCTTC 3 cut(s) 230, 248, 584
EarI CTCTTC 3 cut(s) 230, 248, 584
EclXI CGGCCG 1 cut(s) 462
Eco130I CCWWGG 3 cut(s) 29, 120, 618
Eco147I AGGCCT 1 cut(s) 158
Eco32I GATATC 1 cut(s) 304
Eco47I GGWCC 2 cut(s) 43, 238
Eco52I CGGCCG 1 cut(s) 462
Eco72I CACGTG 2 cut(s) 196, 480
Eco81I CCTNAGG 1 cut(s) 522
Eco88I CYCGRG 1 cut(s) 586
EcoO109I RGGNCCY 2 cut(s) 43, 238
EcoRII CCWGG 1 cut(s) 339
EcoRV GATATC 1 cut(s) 304
EcoT14I CCWWGG 3 cut(s) 29, 120, 618
EgeI GGCGCC 1 cut(s) 10
EheI GGCGCC 1 cut(s) 10
ErhI CCWWGG 3 cut(s) 29, 120, 618
FaeI CATG 6 cut(s) 169, 185, 253, 429, 475, 596
FalI AAGNNNNNCTT 2 cut(s) 146, 178
FaqI GGGAC 2 cut(s) 29, 715
FatI CATG 6 cut(s) 165, 181, 249, 425, 471, 592
FauI CCCGC 2 cut(s) 91, 329
Fnu4HI GCNGC 4 cut(s) 179, 293, 442, 462
FokI GGATG 4 cut(s) 4, 286, 476, 601
Fsp4HI GCNGC 4 cut(s) 179, 293, 442, 462
FspBI CTAG 2 cut(s) 30, 431
GlaI GCGC 2 cut(s) 10, 35
GluI GCNGC 4 cut(s) 179, 293, 442, 462
GsaI CCCAGC 1 cut(s) 701
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 7 cut(s) 125, 158, 200, 441, 464, 731, 770
HapII CCGG 4 cut(s) 271, 484, 600, 771
HgaI GACGC 2 cut(s) 55, 194
HhaI GCGC 2 cut(s) 11, 36
Hin1I GRCGYC 2 cut(s) 9, 186
Hin1II CATG 6 cut(s) 169, 185, 253, 429, 475, 596
Hin6I GCGC 2 cut(s) 9, 34
HinP1I GCGC 2 cut(s) 9, 34
HindIII AAGCTT 1 cut(s) 356
HinfI GANTC 1 cut(s) 761
HpaII CCGG 4 cut(s) 271, 484, 600, 771
HphI GGTGA 2 cut(s) 307, 700
Hpy166II GTNNAC 4 cut(s) 193, 264, 477, 657
Hpy188III TCNNGA 4 cut(s) 166, 271, 540, 559
Hpy8I GTNNAC 4 cut(s) 193, 264, 477, 657
Hpy99I CGWCG 4 cut(s) 71, 286, 289, 292
HpyAV CCTTC 2 cut(s) 87, 172
HpyCH4III ACNGT 1 cut(s) 388
HpyCH4IV ACGT 4 cut(s) 170, 195, 410, 479
HpyCH4V TGCA 6 cut(s) 181, 403, 614, 637, 668, 682
HpyF10VI GCNNNNNNNGC 1 cut(s) 495
HpyF3I CTNAG 1 cut(s) 522
HpySE526I ACGT 4 cut(s) 170, 195, 410, 479
Hsp92I GRCGYC 2 cut(s) 9, 186
Hsp92II CATG 6 cut(s) 169, 185, 253, 429, 475, 596
HspAI GCGC 2 cut(s) 9, 34
KasI GGCGCC 1 cut(s) 8
KflI GGGWCCC 1 cut(s) 43
Kpn2I TCCGGA 1 cut(s) 270
Kzo9I GATC 2 cut(s) 132, 794
LmnI GCTCC 4 cut(s) 273, 329, 542, 743
Lsp1109I GCAGC 1 cut(s) 165
LweI GCATC 3 cut(s) 498, 623, 655
MaeI CTAG 2 cut(s) 30, 431
MaeII ACGT 4 cut(s) 170, 195, 410, 479
MaeIII GTNAC 1 cut(s) 313
MalI GATC 2 cut(s) 134, 796
MbiI CCGCTC 2 cut(s) 324, 545
MboI GATC 2 cut(s) 132, 794
MhlI GDGCHC 1 cut(s) 714
MlsI TGGCCA 1 cut(s) 200
MluNI TGGCCA 1 cut(s) 200
Mly113I GGCGCC 1 cut(s) 9
MmeI TCCRAC 1 cut(s) 721
Mox20I TGGCCA 1 cut(s) 200
MroI TCCGGA 1 cut(s) 270
MscI TGGCCA 1 cut(s) 200
MseI TTAA 2 cut(s) 360, 381
MslI CAYNNNNRTG 1 cut(s) 254
Msp20I TGGCCA 1 cut(s) 200
MspI CCGG 4 cut(s) 271, 484, 600, 771
MspR9I CCNGG 3 cut(s) 341, 485, 601
Mva1269I GAATGC 1 cut(s) 639
MvaI CCWGG 1 cut(s) 341
MvnI CGCG 2 cut(s) 66, 461
MwoI GCNNNNNNNGC 1 cut(s) 495
NarI GGCGCC 1 cut(s) 9
NciI CCSGG 2 cut(s) 485, 601
NdeII GATC 2 cut(s) 132, 794
NlaIII CATG 6 cut(s) 169, 185, 253, 429, 475, 596
NlaIV GGNNCC 5 cut(s) 10, 44, 45, 239, 279
NmeAIII GCCGAG 1 cut(s) 474
NmuCI GTSAC 1 cut(s) 313
NspI RCATGY 1 cut(s) 596
PagI TCATGA 1 cut(s) 165
PceI AGGCCT 1 cut(s) 158
PcsI WCGNNNNNNNCGW 2 cut(s) 192, 293
PctI GAATGC 1 cut(s) 639
PfeI GAWTC 1 cut(s) 761
PflFI GACNNNGTC 1 cut(s) 188
PkrI GCNGC 4 cut(s) 180, 294, 443, 463
PluTI GGCGCC 1 cut(s) 12
PmaCI CACGTG 2 cut(s) 196, 480
PmlI CACGTG 2 cut(s) 196, 480
Ppu21I YACGTR 3 cut(s) 196, 411, 480
PpuMI RGGWCCY 2 cut(s) 43, 238
Psp5II RGGWCCY 2 cut(s) 43, 238
Psp6I CCWGG 1 cut(s) 339
PspCI CACGTG 2 cut(s) 196, 480
PspFI CCCAGC 1 cut(s) 697
PspGI CCWGG 1 cut(s) 339
PspN4I GGNNCC 5 cut(s) 10, 44, 45, 239, 279
PspPI GGNCC 3 cut(s) 43, 238, 439
PspPPI RGGWCCY 2 cut(s) 43, 238
PsyI GACNNNGTC 1 cut(s) 188
RsaI GTAC 2 cut(s) 263, 779
RsaNI GTAC 2 cut(s) 262, 778
RseI CAYNNNNRTG 1 cut(s) 254
SaqAI TTAA 2 cut(s) 360, 381
SatI GCNGC 4 cut(s) 179, 293, 442, 462
Sau3AI GATC 2 cut(s) 132, 794
Sau96I GGNCC 3 cut(s) 43, 238, 439
ScrFI CCNGG 3 cut(s) 341, 485, 601
SduI GDGCHC 1 cut(s) 714
SfaNI GCATC 3 cut(s) 498, 623, 655
SfoI GGCGCC 1 cut(s) 10
SinI GGWCC 2 cut(s) 43, 238
SmiMI CAYNNNNRTG 1 cut(s) 254
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
SseBI AGGCCT 1 cut(s) 158
SsiI CCGC 7 cut(s) 84, 147, 293, 322, 442, 461, 545
SspDI GGCGCC 1 cut(s) 8
SspMI CTAG 2 cut(s) 30, 431
StuI AGGCCT 1 cut(s) 158
StyD4I CCNGG 3 cut(s) 339, 483, 599
StyI CCWWGG 3 cut(s) 29, 120, 618
TaaI ACNGT 1 cut(s) 388
TaiI ACGT 4 cut(s) 173, 198, 413, 482
TaqI TCGA 2 cut(s) 300, 663
TatI WGTACW 1 cut(s) 261
TauI GCSGC 3 cut(s) 295, 444, 464
TfiI GAWTC 1 cut(s) 761
Tru1I TTAA 2 cut(s) 360, 381
Tru9I TTAA 2 cut(s) 360, 381
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 1 cut(s) 178
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 5 cut(s) 6, 17, 154, 238, 460
Tth111I GACNNNGTC 1 cut(s) 188
VpaK11BI GGWCC 2 cut(s) 43, 238
XceI RCATGY 1 cut(s) 596
XmaJI CCTAGG 1 cut(s) 29
XspI CTAG 2 cut(s) 30, 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.