RLG00000019458

Phosphatidylcholine diacylglycerol cholinephosphotransferase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014419
Physical Location & Seq
Forward (+)
52217523 .. 52219026
1504 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019458

Sequence Viewer

Length: 834 bp
ATGAACGGCACCGTTTCGGCCCTCACTTCTCGGCGCCACAAACGAGCCGCCGCCGCCGCCACAAACGGCCCCACCCCTACCGCCGCCATGTCCAAGAAGCTCCTCCCCTACGGCTGCGGCAACAAGGCCTCCTTCATGACGTGGACGGTGCATGACGCCGTCCACGTCGCCAAGCACCACTGGATACCCTGCTTCTTCGCAATGGGCCTCCTCTTCTTCATGGGCGTCGAGTACACGCTCCGCATGGTGCCCTCGTCGGCGCCGCCCTTCGATATCGGCTTCGTCACCACGCGCTCCCTCCACCTCCTCCTGGCCTCGAGCCCCCGGCTCAACACATTGCTCGCTGGCCTCAACACGGTGTTTGTGGGAATGCAGACAACGTATATATTGTGGACATGGCTAATCGAGGGCCGTCCGAGAGCCACCATTGCCACCCTATTTATGTTCACTTGCCGGGGAATCCTTGGCTACTCCACCCAACTTCCATTGCCTCAGGGATTTTTGGGATCGGGAGCAGACTTCCCGGTTGGGAATGTATCGTTTTTCTTGTTCTACTCTGGGCATGTAGCAGGGTCTGTGATTGCATCCCTTGACATGAGAAGAATGCAGAGGTGGGAATTGGCATGGACATTTGATGCATTAAATGTGTTGCAAGCTGTGAGATTGCTAGGGACTAGAGGACACTACACAATAGACTTGGCGGTTGGGGTTGGGGCCGGTATTCTCTTTGATTCTCTGGCAGGGAAGTATGAAGAAAGCAAGAGATTAAATTCAAAAGTAGCTTCAACTACTAGTAATGGTGCCAAAGAGGCTTTGTTTCCATTAGCAGATTAG

Protein Analysis

278

Amino Acids

30.14

Weight (kDa)

9.81

Isoelectric Point (pI)

34.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AtPDCT1_2 PF24788 90 - 249 1.7e-91 AtPDCT1/2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014419)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15820
fragaria_vesca FvH4_6g29670
malus_domestica MD17G1212200.v1.1
prunus_persica Prupe.3G087400_v2.0.a1 Prupe.3G087500_v2.0.a1
pyrus_communis pycom17g21710
rosa_chinensis RchiOBHm_Chr2g0135261
rosa_laevigata RLG00000019458
rosa_multiflora Rmu_sc0003835.1_g000021 Rmu_ssc0000187.1_g000016
rosa_roxburghii Rroxscaffold_2G00109330
rosa_rugosa Rorug02G0324400
rosa_samantha Rh2AG370700 Rh2BG380900 Rh2CG360200 Rh2DG397400
rosa_wichuraiana Rw2G030630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 5 cut(s) 8, 33, 247, 259, 800
AccII CGCG 1 cut(s) 292
AclWI GGATC 1 cut(s) 514
AcsI RAATTY 1 cut(s) 769
AcyI GRCGYC 4 cut(s) 34, 156, 225, 260
AfaI GTAC 1 cut(s) 233
AfiI CCNNNNNNNGG 2 cut(s) 310, 355
AgsI TTSAA 2 cut(s) 774, 786
AhlI ACTAGT 1 cut(s) 791
AjiI CACGTC 2 cut(s) 141, 166
AjnI CCWGG 1 cut(s) 309
AluBI AGCT 3 cut(s) 100, 656, 782
AluI AGCT 3 cut(s) 100, 656, 782
AlwI GGATC 1 cut(s) 514
AlwNI CAGNNNCTG 1 cut(s) 575
Ama87I CYCGRG 1 cut(s) 316
AoxI GGCC 8 cut(s) 18, 67, 126, 205, 312, 346, 409, 714
ApeKI GCWGC 1 cut(s) 114
ApoI RAATTY 1 cut(s) 769
AspLEI GCGC 3 cut(s) 36, 262, 294
AspS9I GGNCC 5 cut(s) 19, 68, 205, 409, 714
AsuC2I CCSGG 3 cut(s) 325, 455, 524
AsuHPI GGTGA 1 cut(s) 277
AvaI CYCGRG 1 cut(s) 316
AxyI CCTNAGG 1 cut(s) 492
BaeGI GKGCMC 1 cut(s) 252
BanI GGYRCC 5 cut(s) 8, 33, 247, 259, 800
BanII GRGCYC 1 cut(s) 323
BbvI GCAGC 1 cut(s) 101
BceAI ACGGC 5 cut(s) 22, 82, 127, 143, 396
BciT130I CCWGG 1 cut(s) 311
BciVI GTATCC 1 cut(s) 177
BcnI CCSGG 3 cut(s) 325, 455, 524
BcuI ACTAGT 1 cut(s) 791
BfaI CTAG 3 cut(s) 668, 675, 792
BfoI RGCGCY 2 cut(s) 37, 263
BfuI GTATCC 1 cut(s) 177
BglI GCCNNNNNGGC 1 cut(s) 809
BisI GCNGC 8 cut(s) 48, 51, 54, 57, 84, 115, 118, 263
BlsI GCNGC 8 cut(s) 49, 52, 55, 58, 85, 116, 119, 264
Bme1390I CCNGG 4 cut(s) 311, 325, 455, 524
BmeT110I CYCGRG 1 cut(s) 316
BmgBI CACGTC 2 cut(s) 141, 166
BmgT120I GGNCC 5 cut(s) 19, 68, 205, 409, 714
BmiI GGNNCC 7 cut(s) 10, 35, 70, 249, 261, 715, 802
BmrFI CCNGG 4 cut(s) 311, 325, 455, 524
BmsI GCATC 2 cut(s) 593, 625
BpuMI CCSGG 3 cut(s) 325, 455, 524
BsaHI GRCGYC 4 cut(s) 34, 156, 225, 260
BsaJI CCNNGG 3 cut(s) 323, 454, 463
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 2 cut(s) 310, 355
Bse118I RCCGGY 1 cut(s) 716
Bse1I ACTGG 1 cut(s) 185
Bse21I CCTNAGG 1 cut(s) 492
Bse3DI GCAATG 4 cut(s) 207, 335, 426, 485
BseBI CCWGG 1 cut(s) 311
BseDI CCNNGG 3 cut(s) 323, 454, 463
BseGI GGATG 1 cut(s) 584
BseLI CCNNNNNNNGG 2 cut(s) 310, 355
BseMI GCAATG 4 cut(s) 207, 335, 426, 485
BseMII CTCAG 1 cut(s) 506
BseNI ACTGG 1 cut(s) 185
BseRI GAGGAG 3 cut(s) 92, 200, 296
BseSI GKGCMC 1 cut(s) 252
BseXI GCAGC 1 cut(s) 101
Bsh1236I CGCG 1 cut(s) 292
BshFI GGCC 8 cut(s) 20, 69, 128, 207, 314, 348, 411, 716
BshNI GGYRCC 5 cut(s) 8, 33, 247, 259, 800
BsiHKCI CYCGRG 1 cut(s) 316
BsiSI CCGG 4 cut(s) 325, 454, 524, 717
BslFI GGGAC 1 cut(s) 685
BslI CCNNNNNNNGG 2 cut(s) 310, 355
BsmFI GGGAC 1 cut(s) 685
BsmI GAATGC 2 cut(s) 375, 609
BsnI GGCC 8 cut(s) 20, 69, 128, 207, 314, 348, 411, 716
BsoBI CYCGRG 1 cut(s) 316
Bsp1286I GDGCHC 2 cut(s) 252, 323
Bsp143I GATC 1 cut(s) 506
BspANI GGCC 8 cut(s) 20, 69, 128, 207, 314, 348, 411, 716
BspCNI CTCAG 1 cut(s) 505
BspFNI CGCG 1 cut(s) 292
BspHI TCATGA 1 cut(s) 135
BspLI GGNNCC 7 cut(s) 10, 35, 70, 249, 261, 715, 802
BspPI GGATC 1 cut(s) 514
BspT107I GGYRCC 5 cut(s) 8, 33, 247, 259, 800
BsrDI GCAATG 4 cut(s) 207, 335, 426, 485
BsrFI RCCGGY 1 cut(s) 716
BsrI ACTGG 1 cut(s) 185
BssAI RCCGGY 1 cut(s) 716
BssECI CCNNGG 3 cut(s) 323, 454, 463
BssMI GATC 1 cut(s) 506
BssNI GRCGYC 4 cut(s) 34, 156, 225, 260
BssT1I CCWWGG 1 cut(s) 463
Bst2UI CCWGG 1 cut(s) 311
Bst4CI ACNGT 3 cut(s) 13, 148, 358
Bst6I CTCTTC 1 cut(s) 218
BstACI GRCGYC 4 cut(s) 34, 156, 225, 260
BstC8I GCNNGC 3 cut(s) 342, 346, 654
BstDEI CTNAG 1 cut(s) 492
BstF5I GGATG 1 cut(s) 584
BstFNI CGCG 1 cut(s) 292
BstH2I RGCGCY 2 cut(s) 37, 263
BstHHI GCGC 3 cut(s) 36, 262, 294
BstKTI GATC 1 cut(s) 509
BstMBI GATC 1 cut(s) 506
BstMWI GCNNNNNNNGC 4 cut(s) 53, 56, 428, 809
BstNI CCWGG 1 cut(s) 311
BstNSI RCATGY 1 cut(s) 566
BstSCI CCNGG 4 cut(s) 309, 323, 453, 522
BstSLI GKGCMC 1 cut(s) 252
BstUI CGCG 1 cut(s) 292
BstV1I GCAGC 1 cut(s) 101
Bsu36I CCTNAGG 1 cut(s) 492
BsuI GTATCC 1 cut(s) 177
BsuRI GGCC 8 cut(s) 20, 69, 128, 207, 314, 348, 411, 716
BtrI CACGTC 2 cut(s) 141, 166
BtsCI GGATG 1 cut(s) 584
BtsIMutI CAGTG 1 cut(s) 178
Cac8I GCNNGC 3 cut(s) 342, 346, 654
CaiI CAGNNNCTG 1 cut(s) 575
CciI TCATGA 1 cut(s) 135
CfoI GCGC 3 cut(s) 36, 262, 294
Cfr10I RCCGGY 1 cut(s) 716
Cfr13I GGNCC 5 cut(s) 19, 68, 205, 409, 714
CseI GACGC 2 cut(s) 164, 214
Csp6I GTAC 1 cut(s) 232
CviAII CATG 9 cut(s) 88, 136, 152, 220, 244, 396, 563, 595, 624
CviQI GTAC 1 cut(s) 232
DdeI CTNAG 1 cut(s) 492
DinI GGCGCC 2 cut(s) 35, 261
DpnI GATC 1 cut(s) 508
DpnII GATC 1 cut(s) 506
Eam1104I CTCTTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 218
Eco130I CCWWGG 1 cut(s) 463
Eco147I AGGCCT 1 cut(s) 128
Eco24I GRGCYC 1 cut(s) 323
Eco32I GATATC 1 cut(s) 274
Eco81I CCTNAGG 1 cut(s) 492
Eco88I CYCGRG 1 cut(s) 316
EcoRII CCWGG 1 cut(s) 309
EcoRV GATATC 1 cut(s) 274
EcoT14I CCWWGG 1 cut(s) 463
EcoT22I ATGCAT 1 cut(s) 640
EcoT38I GRGCYC 1 cut(s) 323
EgeI GGCGCC 2 cut(s) 35, 261
EheI GGCGCC 2 cut(s) 35, 261
ErhI CCWWGG 1 cut(s) 463
FaeI CATG 9 cut(s) 91, 139, 155, 223, 247, 399, 566, 598, 627
FalI AAGNNNNNCTT 2 cut(s) 116, 148
FaqI GGGAC 1 cut(s) 685
FatI CATG 9 cut(s) 87, 135, 151, 219, 243, 395, 562, 594, 623
Fnu4HI GCNGC 8 cut(s) 48, 51, 54, 57, 84, 115, 118, 263
FokI GGATG 1 cut(s) 571
FriOI GRGCYC 1 cut(s) 323
Fsp4HI GCNGC 8 cut(s) 48, 51, 54, 57, 84, 115, 118, 263
FspBI CTAG 3 cut(s) 668, 675, 792
GlaI GCGC 3 cut(s) 35, 261, 293
GluI GCNGC 8 cut(s) 48, 51, 54, 57, 84, 115, 118, 263
HaeII RGCGCY 2 cut(s) 37, 263
HaeIII GGCC 8 cut(s) 20, 69, 128, 207, 314, 348, 411, 716
HapII CCGG 4 cut(s) 325, 454, 524, 717
HgaI GACGC 2 cut(s) 164, 214
HhaI GCGC 3 cut(s) 36, 262, 294
Hin1I GRCGYC 4 cut(s) 34, 156, 225, 260
Hin1II CATG 9 cut(s) 91, 139, 155, 223, 247, 399, 566, 598, 627
Hin6I GCGC 3 cut(s) 34, 260, 292
HinP1I GCGC 3 cut(s) 34, 260, 292
HinfI GANTC 2 cut(s) 459, 731
HpaII CCGG 4 cut(s) 325, 454, 524, 717
HphI GGTGA 1 cut(s) 277
Hpy166II GTNNAC 5 cut(s) 144, 163, 234, 393, 447
Hpy188I TCNGA 1 cut(s) 417
Hpy188III TCNNGA 2 cut(s) 136, 510
Hpy8I GTNNAC 5 cut(s) 144, 163, 234, 393, 447
Hpy99I CGWCG 3 cut(s) 170, 230, 259
HpyAV CCTTC 2 cut(s) 142, 277
HpyCH4III ACNGT 3 cut(s) 13, 148, 358
HpyCH4IV ACGT 3 cut(s) 140, 165, 380
HpyCH4V TGCA 6 cut(s) 151, 373, 584, 607, 638, 652
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 56, 428, 809
HpyF3I CTNAG 1 cut(s) 492
HpySE526I ACGT 3 cut(s) 140, 165, 380
Hsp92I GRCGYC 4 cut(s) 34, 156, 225, 260
Hsp92II CATG 9 cut(s) 91, 139, 155, 223, 247, 399, 566, 598, 627
HspAI GCGC 3 cut(s) 34, 260, 292
KasI GGCGCC 2 cut(s) 33, 259
Kzo9I GATC 1 cut(s) 506
LmnI GCTCC 4 cut(s) 105, 243, 299, 512
Lsp1109I GCAGC 1 cut(s) 101
LweI GCATC 2 cut(s) 593, 625
MaeI CTAG 3 cut(s) 668, 675, 792
MaeII ACGT 3 cut(s) 140, 165, 380
MaeIII GTNAC 1 cut(s) 283
MalI GATC 1 cut(s) 508
MboI GATC 1 cut(s) 506
MboII GAAGA 5 cut(s) 187, 205, 208, 612, 764
MhlI GDGCHC 2 cut(s) 252, 323
MluCI AATT 2 cut(s) 617, 769
Mly113I GGCGCC 2 cut(s) 34, 260
Mph1103I ATGCAT 1 cut(s) 640
MseI TTAA 2 cut(s) 641, 767
MspI CCGG 4 cut(s) 325, 454, 524, 717
MspR9I CCNGG 4 cut(s) 311, 325, 455, 524
Mva1269I GAATGC 2 cut(s) 375, 609
MvaI CCWGG 1 cut(s) 311
MvnI CGCG 1 cut(s) 292
MwoI GCNNNNNNNGC 4 cut(s) 53, 56, 428, 809
NarI GGCGCC 2 cut(s) 34, 260
NciI CCSGG 3 cut(s) 325, 455, 524
NdeII GATC 1 cut(s) 506
NlaIII CATG 9 cut(s) 91, 139, 155, 223, 247, 399, 566, 598, 627
NlaIV GGNNCC 7 cut(s) 10, 35, 70, 249, 261, 715, 802
NmeAIII GCCGAG 1 cut(s) 10
NmuCI GTSAC 1 cut(s) 283
NsiI ATGCAT 1 cut(s) 640
NspI RCATGY 1 cut(s) 566
PaeR7I CTCGAG 1 cut(s) 316
PagI TCATGA 1 cut(s) 135
PceI AGGCCT 1 cut(s) 128
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PctI GAATGC 2 cut(s) 375, 609
PfeI GAWTC 2 cut(s) 459, 731
PflFI GACNNNGTC 1 cut(s) 158
PkrI GCNGC 8 cut(s) 49, 52, 55, 58, 85, 116, 119, 264
PluTI GGCGCC 2 cut(s) 37, 263
Psp6I CCWGG 1 cut(s) 309
PspGI CCWGG 1 cut(s) 309
PspN4I GGNNCC 7 cut(s) 10, 35, 70, 249, 261, 715, 802
PspPI GGNCC 5 cut(s) 19, 68, 205, 409, 714
PspXI VCTCGAGB 1 cut(s) 316
PstNI CAGNNNCTG 1 cut(s) 575
PsyI GACNNNGTC 1 cut(s) 158
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
SaqAI TTAA 2 cut(s) 641, 767
SatI GCNGC 8 cut(s) 48, 51, 54, 57, 84, 115, 118, 263
Sau3AI GATC 1 cut(s) 506
Sau96I GGNCC 5 cut(s) 19, 68, 205, 409, 714
ScrFI CCNGG 4 cut(s) 311, 325, 455, 524
SduI GDGCHC 2 cut(s) 252, 323
SetI ASST 8 cut(s) 102, 143, 168, 306, 383, 614, 658, 784
SfaNI GCATC 2 cut(s) 593, 625
SfoI GGCGCC 2 cut(s) 35, 261
Sfr274I CTCGAG 1 cut(s) 316
SlaI CTCGAG 1 cut(s) 316
SmlI CTYRAG 1 cut(s) 316
SmoI CTYRAG 1 cut(s) 316
SpeI ACTAGT 1 cut(s) 791
Sse9I AATT 2 cut(s) 617, 769
SseBI AGGCCT 1 cut(s) 128
SspDI GGCGCC 2 cut(s) 33, 259
SspMI CTAG 3 cut(s) 668, 675, 792
StuI AGGCCT 1 cut(s) 128
StyD4I CCNGG 4 cut(s) 309, 323, 453, 522
StyI CCWWGG 1 cut(s) 463
TaaI ACNGT 3 cut(s) 13, 148, 358
TaiI ACGT 3 cut(s) 143, 168, 383
TaqI TCGA 4 cut(s) 228, 270, 317, 405
TasI AATT 2 cut(s) 617, 769
TatI WGTACW 1 cut(s) 231
TauI GCSGC 7 cut(s) 50, 53, 56, 59, 86, 120, 265
TfiI GAWTC 2 cut(s) 459, 731
Tru1I TTAA 2 cut(s) 641, 767
Tru9I TTAA 2 cut(s) 641, 767
TscAI CASTG 1 cut(s) 185
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 1 cut(s) 114
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 4 cut(s) 17, 124, 208, 765
TspRI CASTG 1 cut(s) 185
Tth111I GACNNNGTC 1 cut(s) 158
XapI RAATTY 1 cut(s) 769
XceI RCATGY 1 cut(s) 566
XhoI CTCGAG 1 cut(s) 316
XspI CTAG 3 cut(s) 668, 675, 792
Zsp2I ATGCAT 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.