RchiOBHm_Chr2g0135261

Phosphatidylcholine diacylglycerol cholinephosphotransferase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0014419
Physical Location & Seq
Forward (+)
52319146 .. 52321270
2125 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50620

Sequence Viewer

Length: 837 bp
ATGAACGGCACCGTTTCGGCCCTCACTTCTCGGCGCCACAAACGAGCCGCCGCCGCCGCCACAAACGGCCCCACCCCTACCGCCGCCATGTCCAAGAAGCTCCTCCCCGTGCACGGCTGCGGCAACAAGGCCTCCTTCATGACGTGGACGGTGCATGACGCCGTCCACGTCGCCAAGCACCACTGGATACCCTGCTTCTTCGCAATGGGCCTCCTCTTCTTCATGGGCGTCGAGTACACGCTCCGCATGGTGCCCTCCTCCGCGCCGCCCTTCGATGTCGGCTTCGTCACCACGCGCTCCCTCCACCTCCTCCTGGCCTCGAGCCCCCAGCTCAACACATTGCTCGCTGGCCTCAACACGGTGTTTGTGGGAATGCAGACAACGTATATATTGTGGACGTGGCTAATCGAGGGCCGTCCAAGAGCCACCATCGCCGCCCTATTTATGTTCACTTGCCGGGGAATCCTTGGTTACTCCACCCAACTTCCGTTGCCTCAGGGATTTTTGGGGTCGGGAGCAGACTTCCCGGTTGGGAATGTATCATTTTTCTTGTTCTACTCCGGGCATGTAGCAGGGTCTGTGATTGCATCCCTCGACATGAGAAGAATGCAGAGGTGGGAATTGGCATGGACATTTGATGCATTAAATGTGTTGCAAGCTGTGAGATTGTTAGGGACTAGAGGACACTACACAATAGACTTGGCGGTTGGGGTTGGGGCCGGTATTCTCTTTGATTCTCTGGCAGGGAAGTATGAAGAAAGCAAGAGACTAAATTCAAAAGTAGCTTCAACTACTAGTAATGGTGCCAAAGAGGCTTTGTTTCCATTAGCAGATTAG

Protein Analysis

278

Amino Acids

30.14

Weight (kDa)

9.75

Isoelectric Point (pI)

36.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AtPDCT1_2 PF24788 91 - 250 8.1e-92 AtPDCT1/2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014419)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15820
fragaria_vesca FvH4_6g29670
malus_domestica MD17G1212200.v1.1
prunus_persica Prupe.3G087400_v2.0.a1 Prupe.3G087500_v2.0.a1
pyrus_communis pycom17g21710
rosa_chinensis RchiOBHm_Chr2g0135261
rosa_laevigata RLG00000019458
rosa_multiflora Rmu_sc0003835.1_g000021 Rmu_ssc0000187.1_g000016
rosa_roxburghii Rroxscaffold_2G00109330
rosa_rugosa Rorug02G0324400
rosa_samantha Rh2AG370700 Rh2BG380900 Rh2CG360200 Rh2DG397400
rosa_wichuraiana Rw2G030630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 8, 33, 250, 803
AccII CGCG 2 cut(s) 263, 295
AcsI RAATTY 1 cut(s) 772
AcyI GRCGYC 3 cut(s) 34, 159, 228
AfaI GTAC 1 cut(s) 236
AfiI CCNNNNNNNGG 3 cut(s) 113, 313, 358
AgsI TTSAA 2 cut(s) 777, 789
AhlI ACTAGT 1 cut(s) 794
AjiI CACGTC 3 cut(s) 144, 169, 399
AjnI CCWGG 1 cut(s) 312
AluBI AGCT 4 cut(s) 100, 331, 659, 785
AluI AGCT 4 cut(s) 100, 331, 659, 785
Alw21I GWGCWC 1 cut(s) 114
Alw26I GTCTC 1 cut(s) 760
Alw44I GTGCAC 1 cut(s) 110
AlwNI CAGNNNCTG 1 cut(s) 578
Ama87I CYCGRG 1 cut(s) 319
AoxI GGCC 8 cut(s) 18, 67, 129, 208, 315, 349, 412, 717
ApaLI GTGCAC 1 cut(s) 110
ApeKI GCWGC 1 cut(s) 117
ApoI RAATTY 1 cut(s) 772
AspLEI GCGC 3 cut(s) 36, 265, 297
AspS9I GGNCC 5 cut(s) 19, 68, 208, 412, 717
AsuC2I CCSGG 3 cut(s) 458, 527, 562
AsuHPI GGTGA 1 cut(s) 280
AvaI CYCGRG 1 cut(s) 319
AxyI CCTNAGG 1 cut(s) 495
BaeGI GKGCMC 2 cut(s) 114, 255
BanI GGYRCC 4 cut(s) 8, 33, 250, 803
BanII GRGCYC 1 cut(s) 326
Bbv12I GWGCWC 1 cut(s) 114
BbvI GCAGC 1 cut(s) 104
BccI CCATC 1 cut(s) 437
BceAI ACGGC 5 cut(s) 22, 82, 130, 146, 399
BciT130I CCWGG 1 cut(s) 314
BciVI GTATCC 1 cut(s) 180
BcnI CCSGG 3 cut(s) 458, 527, 562
BcoDI GTCTC 1 cut(s) 760
BcuI ACTAGT 1 cut(s) 794
BfaI CTAG 2 cut(s) 678, 795
BfoI RGCGCY 1 cut(s) 37
BfuI GTATCC 1 cut(s) 180
BglI GCCNNNNNGGC 1 cut(s) 812
BisI GCNGC 9 cut(s) 48, 51, 54, 57, 84, 118, 121, 266, 435
BlsI GCNGC 9 cut(s) 49, 52, 55, 58, 85, 119, 122, 267, 436
Bme1390I CCNGG 4 cut(s) 314, 458, 527, 562
BmeT110I CYCGRG 1 cut(s) 319
BmgBI CACGTC 3 cut(s) 144, 169, 399
BmgT120I GGNCC 5 cut(s) 19, 68, 208, 412, 717
BmiI GGNNCC 6 cut(s) 10, 35, 70, 252, 718, 805
BmrFI CCNGG 4 cut(s) 314, 458, 527, 562
BmsI GCATC 2 cut(s) 596, 628
BpuMI CCSGG 3 cut(s) 458, 527, 562
BsaHI GRCGYC 3 cut(s) 34, 159, 228
BsaJI CCNNGG 2 cut(s) 457, 466
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 3 cut(s) 113, 313, 358
Bse118I RCCGGY 1 cut(s) 719
Bse1I ACTGG 1 cut(s) 188
Bse21I CCTNAGG 1 cut(s) 495
Bse3DI GCAATG 2 cut(s) 210, 338
BseBI CCWGG 1 cut(s) 314
BseDI CCNNGG 2 cut(s) 457, 466
BseGI GGATG 1 cut(s) 587
BseLI CCNNNNNNNGG 3 cut(s) 113, 313, 358
BseMI GCAATG 2 cut(s) 210, 338
BseMII CTCAG 1 cut(s) 509
BseNI ACTGG 1 cut(s) 188
BseRI GAGGAG 4 cut(s) 92, 203, 247, 299
BseSI GKGCMC 2 cut(s) 114, 255
BseXI GCAGC 1 cut(s) 104
BseYI CCCAGC 1 cut(s) 327
Bsh1236I CGCG 2 cut(s) 263, 295
BshFI GGCC 8 cut(s) 20, 69, 131, 210, 317, 351, 414, 719
BshNI GGYRCC 4 cut(s) 8, 33, 250, 803
BsiHKAI GWGCWC 1 cut(s) 114
BsiHKCI CYCGRG 1 cut(s) 319
BsiSI CCGG 4 cut(s) 457, 527, 561, 720
BslFI GGGAC 1 cut(s) 688
BslI CCNNNNNNNGG 3 cut(s) 113, 313, 358
BsmAI GTCTC 1 cut(s) 760
BsmFI GGGAC 1 cut(s) 688
BsmI GAATGC 2 cut(s) 378, 612
BsnI GGCC 8 cut(s) 20, 69, 131, 210, 317, 351, 414, 719
BsoBI CYCGRG 1 cut(s) 319
Bsp1286I GDGCHC 3 cut(s) 114, 255, 326
BspANI GGCC 8 cut(s) 20, 69, 131, 210, 317, 351, 414, 719
BspCNI CTCAG 1 cut(s) 508
BspFNI CGCG 2 cut(s) 263, 295
BspHI TCATGA 1 cut(s) 138
BspLI GGNNCC 6 cut(s) 10, 35, 70, 252, 718, 805
BspT107I GGYRCC 4 cut(s) 8, 33, 250, 803
BsrDI GCAATG 2 cut(s) 210, 338
BsrFI RCCGGY 1 cut(s) 719
BsrI ACTGG 1 cut(s) 188
BssAI RCCGGY 1 cut(s) 719
BssECI CCNNGG 2 cut(s) 457, 466
BssNI GRCGYC 3 cut(s) 34, 159, 228
BssT1I CCWWGG 1 cut(s) 466
Bst2UI CCWGG 1 cut(s) 314
Bst4CI ACNGT 3 cut(s) 13, 151, 361
Bst6I CTCTTC 1 cut(s) 221
BstACI GRCGYC 3 cut(s) 34, 159, 228
BstC8I GCNNGC 3 cut(s) 345, 349, 657
BstDEI CTNAG 1 cut(s) 495
BstF5I GGATG 1 cut(s) 587
BstFNI CGCG 2 cut(s) 263, 295
BstH2I RGCGCY 1 cut(s) 37
BstHHI GCGC 3 cut(s) 36, 265, 297
BstMAI GTCTC 1 cut(s) 760
BstMWI GCNNNNNNNGC 4 cut(s) 53, 56, 431, 812
BstNI CCWGG 1 cut(s) 314
BstNSI RCATGY 1 cut(s) 569
BstSCI CCNGG 4 cut(s) 312, 456, 525, 560
BstSLI GKGCMC 2 cut(s) 114, 255
BstUI CGCG 2 cut(s) 263, 295
BstV1I GCAGC 1 cut(s) 104
Bsu36I CCTNAGG 1 cut(s) 495
BsuI GTATCC 1 cut(s) 180
BsuRI GGCC 8 cut(s) 20, 69, 131, 210, 317, 351, 414, 719
BtgZI GCGATG 1 cut(s) 415
BtrI CACGTC 3 cut(s) 144, 169, 399
BtsCI GGATG 1 cut(s) 587
BtsIMutI CAGTG 1 cut(s) 181
Cac8I GCNNGC 3 cut(s) 345, 349, 657
CaiI CAGNNNCTG 1 cut(s) 578
CciI TCATGA 1 cut(s) 138
CfoI GCGC 3 cut(s) 36, 265, 297
Cfr10I RCCGGY 1 cut(s) 719
Cfr13I GGNCC 5 cut(s) 19, 68, 208, 412, 717
CseI GACGC 2 cut(s) 167, 217
Csp6I GTAC 1 cut(s) 235
CviAII CATG 8 cut(s) 88, 139, 155, 223, 247, 566, 598, 627
CviQI GTAC 1 cut(s) 235
DdeI CTNAG 1 cut(s) 495
DinI GGCGCC 1 cut(s) 35
Eam1104I CTCTTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 221
Eco130I CCWWGG 1 cut(s) 466
Eco147I AGGCCT 1 cut(s) 131
Eco24I GRGCYC 1 cut(s) 326
Eco81I CCTNAGG 1 cut(s) 495
Eco88I CYCGRG 1 cut(s) 319
EcoRII CCWGG 1 cut(s) 312
EcoT14I CCWWGG 1 cut(s) 466
EcoT22I ATGCAT 1 cut(s) 643
EcoT38I GRGCYC 1 cut(s) 326
EgeI GGCGCC 1 cut(s) 35
EheI GGCGCC 1 cut(s) 35
ErhI CCWWGG 1 cut(s) 466
FaeI CATG 8 cut(s) 91, 142, 158, 226, 250, 569, 601, 630
FalI AAGNNNNNCTT 2 cut(s) 119, 151
FaqI GGGAC 1 cut(s) 688
FatI CATG 8 cut(s) 87, 138, 154, 222, 246, 565, 597, 626
Fnu4HI GCNGC 9 cut(s) 48, 51, 54, 57, 84, 118, 121, 266, 435
FokI GGATG 1 cut(s) 574
FriOI GRGCYC 1 cut(s) 326
Fsp4HI GCNGC 9 cut(s) 48, 51, 54, 57, 84, 118, 121, 266, 435
FspBI CTAG 2 cut(s) 678, 795
GlaI GCGC 3 cut(s) 35, 264, 296
GluI GCNGC 9 cut(s) 48, 51, 54, 57, 84, 118, 121, 266, 435
GsaI CCCAGC 1 cut(s) 331
HaeII RGCGCY 1 cut(s) 37
HaeIII GGCC 8 cut(s) 20, 69, 131, 210, 317, 351, 414, 719
HapII CCGG 4 cut(s) 457, 527, 561, 720
HgaI GACGC 2 cut(s) 167, 217
HhaI GCGC 3 cut(s) 36, 265, 297
Hin1I GRCGYC 3 cut(s) 34, 159, 228
Hin1II CATG 8 cut(s) 91, 142, 158, 226, 250, 569, 601, 630
Hin6I GCGC 3 cut(s) 34, 263, 295
HinP1I GCGC 3 cut(s) 34, 263, 295
HinfI GANTC 2 cut(s) 462, 734
HpaII CCGG 4 cut(s) 457, 527, 561, 720
HphI GGTGA 1 cut(s) 280
Hpy166II GTNNAC 6 cut(s) 112, 147, 166, 237, 396, 450
Hpy188III TCNNGA 2 cut(s) 139, 513
Hpy8I GTNNAC 6 cut(s) 112, 147, 166, 237, 396, 450
Hpy99I CGWCG 2 cut(s) 173, 233
HpyAV CCTTC 2 cut(s) 145, 280
HpyCH4III ACNGT 3 cut(s) 13, 151, 361
HpyCH4IV ACGT 4 cut(s) 143, 168, 383, 398
HpyCH4V TGCA 7 cut(s) 112, 154, 376, 587, 610, 641, 655
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 56, 431, 812
HpyF3I CTNAG 1 cut(s) 495
HpySE526I ACGT 4 cut(s) 143, 168, 383, 398
Hsp92I GRCGYC 3 cut(s) 34, 159, 228
Hsp92II CATG 8 cut(s) 91, 142, 158, 226, 250, 569, 601, 630
HspAI GCGC 3 cut(s) 34, 263, 295
KasI GGCGCC 1 cut(s) 33
LmnI GCTCC 4 cut(s) 105, 246, 302, 515
Lsp1109I GCAGC 1 cut(s) 104
LweI GCATC 2 cut(s) 596, 628
MaeI CTAG 2 cut(s) 678, 795
MaeII ACGT 4 cut(s) 143, 168, 383, 398
MaeIII GTNAC 2 cut(s) 286, 470
MboII GAAGA 5 cut(s) 190, 208, 211, 615, 767
MhlI GDGCHC 3 cut(s) 114, 255, 326
MluCI AATT 2 cut(s) 620, 772
Mly113I GGCGCC 1 cut(s) 34
Mph1103I ATGCAT 1 cut(s) 643
MseI TTAA 1 cut(s) 644
MspI CCGG 4 cut(s) 457, 527, 561, 720
MspR9I CCNGG 4 cut(s) 314, 458, 527, 562
Mva1269I GAATGC 2 cut(s) 378, 612
MvaI CCWGG 1 cut(s) 314
MvnI CGCG 2 cut(s) 263, 295
MwoI GCNNNNNNNGC 4 cut(s) 53, 56, 431, 812
NarI GGCGCC 1 cut(s) 34
NciI CCSGG 3 cut(s) 458, 527, 562
NlaIII CATG 8 cut(s) 91, 142, 158, 226, 250, 569, 601, 630
NlaIV GGNNCC 6 cut(s) 10, 35, 70, 252, 718, 805
NmeAIII GCCGAG 1 cut(s) 10
NmuCI GTSAC 1 cut(s) 286
NsiI ATGCAT 1 cut(s) 643
NspI RCATGY 1 cut(s) 569
PaeR7I CTCGAG 1 cut(s) 319
PagI TCATGA 1 cut(s) 138
PceI AGGCCT 1 cut(s) 131
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PctI GAATGC 2 cut(s) 378, 612
PfeI GAWTC 2 cut(s) 462, 734
PflFI GACNNNGTC 1 cut(s) 161
PkrI GCNGC 9 cut(s) 49, 52, 55, 58, 85, 119, 122, 267, 436
PluTI GGCGCC 1 cut(s) 37
Psp6I CCWGG 1 cut(s) 312
PspFI CCCAGC 1 cut(s) 327
PspGI CCWGG 1 cut(s) 312
PspN4I GGNNCC 6 cut(s) 10, 35, 70, 252, 718, 805
PspPI GGNCC 5 cut(s) 19, 68, 208, 412, 717
PspXI VCTCGAGB 1 cut(s) 319
PstNI CAGNNNCTG 1 cut(s) 578
PsyI GACNNNGTC 1 cut(s) 161
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
SaqAI TTAA 1 cut(s) 644
SatI GCNGC 9 cut(s) 48, 51, 54, 57, 84, 118, 121, 266, 435
Sau96I GGNCC 5 cut(s) 19, 68, 208, 412, 717
ScrFI CCNGG 4 cut(s) 314, 458, 527, 562
SduI GDGCHC 3 cut(s) 114, 255, 326
SfaNI GCATC 2 cut(s) 596, 628
SfoI GGCGCC 1 cut(s) 35
Sfr274I CTCGAG 1 cut(s) 319
SlaI CTCGAG 1 cut(s) 319
SmlI CTYRAG 1 cut(s) 319
SmoI CTYRAG 1 cut(s) 319
SpeI ACTAGT 1 cut(s) 794
Sse9I AATT 2 cut(s) 620, 772
SseBI AGGCCT 1 cut(s) 131
SspDI GGCGCC 1 cut(s) 33
SspMI CTAG 2 cut(s) 678, 795
StuI AGGCCT 1 cut(s) 131
StyD4I CCNGG 4 cut(s) 312, 456, 525, 560
StyI CCWWGG 1 cut(s) 466
TaaI ACNGT 3 cut(s) 13, 151, 361
TaiI ACGT 4 cut(s) 146, 171, 386, 401
TaqI TCGA 5 cut(s) 231, 273, 320, 408, 594
TasI AATT 2 cut(s) 620, 772
TatI WGTACW 1 cut(s) 234
TauI GCSGC 8 cut(s) 50, 53, 56, 59, 86, 123, 268, 437
TfiI GAWTC 2 cut(s) 462, 734
Tru1I TTAA 1 cut(s) 644
Tru9I TTAA 1 cut(s) 644
TscAI CASTG 1 cut(s) 188
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 1 cut(s) 117
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 4 cut(s) 17, 127, 211, 768
TspGWI ACGGA 1 cut(s) 477
TspRI CASTG 1 cut(s) 188
Tth111I GACNNNGTC 1 cut(s) 161
VneI GTGCAC 1 cut(s) 110
XapI RAATTY 1 cut(s) 772
XceI RCATGY 1 cut(s) 569
XhoI CTCGAG 1 cut(s) 319
XspI CTAG 2 cut(s) 678, 795
Zsp2I ATGCAT 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.