MD17G1221100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007160
Physical Location & Seq
Forward (+)
26994485 .. 26997640
3156 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1221100.v1.1.491

Sequence Viewer

Length: 765 bp
ATGTTTCGCGCCAGAGAAATCTTGGTCCGGCTCCCTAAACGTTTAAAACCCACAACAATCTTACAACCAAGGAGAACATTTTGCTCCAAATCCGCTAAAAATGCCAACAATACTAACGCCGAGGAGACGAGTGTGAGTTCGTACCAGGAGCAGTATAAGCAGCTGGAGAAGCTCGACTTCATGACCGCCACCAAAATGCTCTTCACTGCTCCGCCTTCGAAAAAGAAATTTGGGCTCGATTTCCATTTGGTACAACTGTTCTTTTGTTGCATGCCTTCCTTGGCTGTGTATCTGGTGGCTCAGTATGCTCGCTATGAAATTAGAAGAATGGAAGCGGAACAGGAGATGAAAAAGAAGAAAGAAGAAGAAACGAAAGCAAAAGAAATAGAAGTAAATGCTACCGAAGAAAAAGAAGCAGGATCTGTTCCAGAGCTTTTGGAGGTAAAAGTGAGACTAGATAAACTTGAAGAAGCACTGAAGGAAATAGTGGTTGAATCGAAGAAGCAAACTGCCAGCGGGTTAACGAAAGATCAGGAAGTAGGTAGTGAGAAGAAAGATGGTGTAAAACAGGCAACTGACACTAGTAGTTCAGACTCCAGCAAGCCTTCTGAGAAAGATCACCTTAGTAAACAGGATTCTGGAGAATCAAGACCGGCCCTCGGCAAAGAGAGAGTAAATGGTCAGCACCCGGTTCGTAATGCTTCCTTGAAGGATCAGCTGGACAAAACCGAAGATGGAGGGGCTTCTCAAGATGCTAAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Musclin

Protein Analysis

255

Amino Acids

28.71

Weight (kDa)

8.88

Isoelectric Point (pI)

57.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 9
AciI CCGC 5 cut(s) 93, 186, 212, 335, 516
AclI AACGTT 1 cut(s) 40
AclWI GGATC 2 cut(s) 427, 720
AcsI RAATTY 1 cut(s) 227
AcuI CTGAAG 1 cut(s) 497
AfaI GTAC 2 cut(s) 143, 252
AfiI CCNNNNNNNGG 1 cut(s) 659
AgsI TTSAA 3 cut(s) 467, 494, 709
AhlI ACTAGT 1 cut(s) 581
AjnI CCWGG 1 cut(s) 144
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
AluBI AGCT 4 cut(s) 163, 172, 433, 718
AluI AGCT 4 cut(s) 163, 172, 433, 718
Alw26I GTCTC 2 cut(s) 119, 445
AlwI GGATC 2 cut(s) 427, 720
AlwNI CAGNNNCTG 1 cut(s) 422
AoxI GGCC 1 cut(s) 654
ApeKI GCWGC 1 cut(s) 160
ApoI RAATTY 1 cut(s) 227
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 2 cut(s) 25, 655
AsuC2I CCSGG 1 cut(s) 689
AsuHPI GGTGA 1 cut(s) 611
AsuII TTCGAA 1 cut(s) 218
AvaII GGWCC 1 cut(s) 25
BaeI ACNNNNGTAYC 2 cut(s) 242, 275
BanII GRGCYC 1 cut(s) 237
BbvI GCAGC 1 cut(s) 172
BccI CCATC 2 cut(s) 551, 728
BcgI CGANNNNNNTGC 2 cut(s) 674, 708
BciT130I CCWGG 1 cut(s) 146
BcnI CCSGG 1 cut(s) 689
BcoDI GTCTC 2 cut(s) 119, 445
BcuI ACTAGT 1 cut(s) 581
BfaI CTAG 2 cut(s) 455, 582
BisI GCNGC 1 cut(s) 161
BlsI GCNGC 1 cut(s) 162
Bme1390I CCNGG 2 cut(s) 146, 689
Bme18I GGWCC 1 cut(s) 25
BmgT120I GGNCC 2 cut(s) 25, 655
BmiI GGNNCC 1 cut(s) 32
BmrFI CCNGG 2 cut(s) 146, 689
BmsI GCATC 1 cut(s) 742
BpmI CTGGAG 3 cut(s) 185, 580, 660
Bpu14I TTCGAA 1 cut(s) 218
BpuEI CTTGAG 1 cut(s) 732
BpuMI CCSGG 1 cut(s) 689
BsaJI CCNNGG 4 cut(s) 68, 120, 279, 658
Bsc4I CCNNNNNNNGG 1 cut(s) 659
Bse118I RCCGGY 1 cut(s) 652
BseBI CCWGG 1 cut(s) 146
BseDI CCNNGG 4 cut(s) 68, 120, 279, 658
BseLI CCNNNNNNNGG 1 cut(s) 659
BseMII CTCAG 2 cut(s) 314, 600
BseRI GAGGAG 1 cut(s) 137
BseXI GCAGC 1 cut(s) 172
Bsh1236I CGCG 1 cut(s) 9
BshFI GGCC 1 cut(s) 656
BsiSI CCGG 3 cut(s) 28, 653, 689
BslI CCNNNNNNNGG 1 cut(s) 659
BsmAI GTCTC 2 cut(s) 119, 445
BsmBI CGTCTC 1 cut(s) 119
BsnI GGCC 1 cut(s) 656
Bsp119I TTCGAA 1 cut(s) 218
Bsp1286I GDGCHC 1 cut(s) 237
Bsp143I GATC 4 cut(s) 419, 529, 616, 712
BspACI CCGC 5 cut(s) 93, 186, 212, 335, 516
BspANI GGCC 1 cut(s) 656
BspCNI CTCAG 2 cut(s) 313, 601
BspFNI CGCG 1 cut(s) 9
BspHI TCATGA 1 cut(s) 180
BspLI GGNNCC 1 cut(s) 32
BspPI GGATC 2 cut(s) 427, 720
BspQI GCTCTTC 1 cut(s) 206
BspT104I TTCGAA 1 cut(s) 218
BsrFI RCCGGY 1 cut(s) 652
BssAI RCCGGY 1 cut(s) 652
BssECI CCNNGG 4 cut(s) 68, 120, 279, 658
BssMI GATC 4 cut(s) 419, 529, 616, 712
BssT1I CCWWGG 2 cut(s) 68, 279
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 1 cut(s) 258
Bst6I CTCTTC 1 cut(s) 206
BstBI TTCGAA 1 cut(s) 218
BstC8I GCNNGC 4 cut(s) 272, 310, 514, 602
BstDEI CTNAG 3 cut(s) 300, 609, 623
BstFNI CGCG 1 cut(s) 9
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 4 cut(s) 422, 532, 619, 715
BstMAI GTCTC 2 cut(s) 119, 445
BstMBI GATC 4 cut(s) 419, 529, 616, 712
BstMWI GCNNNNNNNGC 4 cut(s) 101, 157, 169, 305
BstNI CCWGG 1 cut(s) 146
BstNSI RCATGY 1 cut(s) 274
BstSCI CCNGG 2 cut(s) 144, 687
BstUI CGCG 1 cut(s) 9
BstV1I GCAGC 1 cut(s) 172
BstX2I RGATCY 1 cut(s) 419
BstYI RGATCY 1 cut(s) 419
BsuRI GGCC 1 cut(s) 656
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 2 cut(s) 204, 473
Cac8I GCNNGC 4 cut(s) 272, 310, 514, 602
CaiI CAGNNNCTG 1 cut(s) 422
CciI TCATGA 1 cut(s) 180
CfoI GCGC 1 cut(s) 11
Cfr10I RCCGGY 1 cut(s) 652
Cfr13I GGNCC 2 cut(s) 25, 655
Csp6I GTAC 2 cut(s) 142, 251
CviAII CATG 2 cut(s) 181, 271
CviQI GTAC 2 cut(s) 142, 251
DdeI CTNAG 3 cut(s) 300, 609, 623
DpnI GATC 4 cut(s) 421, 531, 618, 714
DpnII GATC 4 cut(s) 419, 529, 616, 712
DraI TTTAAA 1 cut(s) 45
Eam1104I CTCTTC 1 cut(s) 206
EarI CTCTTC 1 cut(s) 206
EciI GGCGGA 1 cut(s) 201
Eco130I CCWWGG 2 cut(s) 68, 279
Eco24I GRGCYC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 497
EcoRII CCWGG 1 cut(s) 144
EcoT14I CCWWGG 2 cut(s) 68, 279
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 2 cut(s) 68, 279
Esp3I CGTCTC 1 cut(s) 119
FaeI CATG 2 cut(s) 184, 274
FaiI YATR 5 cut(s) 156, 182, 272, 306, 315
FalI AAGNNNNNCTT 4 cut(s) 161, 193, 606, 638
FatI CATG 2 cut(s) 180, 270
FauI CCCGC 1 cut(s) 509
Fnu4HI GCNGC 1 cut(s) 161
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 1 cut(s) 161
FspBI CTAG 2 cut(s) 455, 582
GlaI GCGC 1 cut(s) 10
GluI GCNGC 1 cut(s) 161
GsuI CTGGAG 3 cut(s) 185, 580, 660
HaeIII GGCC 1 cut(s) 656
HapII CCGG 3 cut(s) 28, 653, 689
HhaI GCGC 1 cut(s) 11
Hin1II CATG 2 cut(s) 184, 274
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HincII GTYRAC 1 cut(s) 522
HindII GTYRAC 1 cut(s) 522
HinfI GANTC 4 cut(s) 494, 593, 635, 644
HpaI GTTAAC 1 cut(s) 522
HpaII CCGG 3 cut(s) 28, 653, 689
HphI GGTGA 1 cut(s) 611
Hpy166II GTNNAC 2 cut(s) 522, 629
Hpy188I TCNGA 2 cut(s) 592, 610
Hpy188III TCNNGA 6 cut(s) 181, 428, 533, 639, 648, 749
Hpy8I GTNNAC 2 cut(s) 522, 629
HpyAV CCTTC 5 cut(s) 225, 285, 472, 615, 703
HpyCH4III ACNGT 1 cut(s) 258
HpyCH4IV ACGT 1 cut(s) 40
HpyCH4V TGCA 1 cut(s) 270
HpyF10VI GCNNNNNNNGC 4 cut(s) 101, 157, 169, 305
HpyF3I CTNAG 3 cut(s) 300, 609, 623
HpySE526I ACGT 1 cut(s) 40
Hsp92II CATG 2 cut(s) 184, 274
HspAI GCGC 1 cut(s) 9
KspAI GTTAAC 1 cut(s) 522
Kzo9I GATC 4 cut(s) 419, 529, 616, 712
LguI GCTCTTC 1 cut(s) 206
LmnI GCTCC 4 cut(s) 36, 89, 148, 214
Lsp1109I GCAGC 1 cut(s) 172
LweI GCATC 1 cut(s) 742
MaeI CTAG 2 cut(s) 455, 582
MaeII ACGT 1 cut(s) 40
MalI GATC 4 cut(s) 421, 531, 618, 714
MboI GATC 4 cut(s) 419, 529, 616, 712
MflI RGATCY 1 cut(s) 419
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 2 cut(s) 227, 318
MlyI GAGTC 1 cut(s) 587
MnlI CCTC 4 cut(s) 115, 433, 668, 731
MseI TTAA 2 cut(s) 44, 521
MslI CAYNNNNRTG 1 cut(s) 194
MspA1I CMGCKG 3 cut(s) 163, 516, 718
MspI CCGG 3 cut(s) 28, 653, 689
MspR9I CCNGG 2 cut(s) 146, 689
MvaI CCWGG 1 cut(s) 146
MvnI CGCG 1 cut(s) 9
MwoI GCNNNNNNNGC 4 cut(s) 101, 157, 169, 305
NciI CCSGG 1 cut(s) 689
NdeII GATC 4 cut(s) 419, 529, 616, 712
NlaIII CATG 2 cut(s) 184, 274
NlaIV GGNNCC 1 cut(s) 32
NmeAIII GCCGAG 2 cut(s) 145, 639
NspI RCATGY 1 cut(s) 274
NspV TTCGAA 1 cut(s) 218
PaeI GCATGC 1 cut(s) 274
PagI TCATGA 1 cut(s) 180
PciSI GCTCTTC 1 cut(s) 206
PfeI GAWTC 3 cut(s) 494, 635, 644
PkrI GCNGC 1 cut(s) 162
PleI GAGTC 1 cut(s) 587
PpsI GAGTC 1 cut(s) 587
Psp1406I AACGTT 1 cut(s) 40
Psp6I CCWGG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 144
PspN4I GGNNCC 1 cut(s) 32
PspPI GGNCC 2 cut(s) 25, 655
PstNI CAGNNNCTG 1 cut(s) 422
PsuI RGATCY 1 cut(s) 419
PvuII CAGCTG 2 cut(s) 163, 718
RsaI GTAC 2 cut(s) 143, 252
RsaNI GTAC 2 cut(s) 142, 251
RseI CAYNNNNRTG 1 cut(s) 194
SapI GCTCTTC 1 cut(s) 206
SaqAI TTAA 2 cut(s) 44, 521
SatI GCNGC 1 cut(s) 161
Sau3AI GATC 4 cut(s) 419, 529, 616, 712
Sau96I GGNCC 2 cut(s) 25, 655
SchI GAGTC 1 cut(s) 587
ScrFI CCNGG 2 cut(s) 146, 689
SduI GDGCHC 1 cut(s) 237
SetI ASST 8 cut(s) 43, 165, 174, 435, 444, 544, 624, 720
SfaNI GCATC 1 cut(s) 742
SfuI TTCGAA 1 cut(s) 218
SinI GGWCC 1 cut(s) 25
SmiMI CAYNNNNRTG 1 cut(s) 194
SmlI CTYRAG 1 cut(s) 747
SmoI CTYRAG 1 cut(s) 747
SpeI ACTAGT 1 cut(s) 581
SphI GCATGC 1 cut(s) 274
Sse9I AATT 2 cut(s) 227, 318
SsiI CCGC 5 cut(s) 93, 186, 212, 335, 516
SspMI CTAG 2 cut(s) 455, 582
StyD4I CCNGG 2 cut(s) 144, 687
StyI CCWWGG 2 cut(s) 68, 279
TaaI ACNGT 1 cut(s) 258
TaiI ACGT 1 cut(s) 43
TaqI TCGA 4 cut(s) 174, 218, 237, 497
TasI AATT 2 cut(s) 227, 318
TfiI GAWTC 3 cut(s) 494, 635, 644
Tru1I TTAA 2 cut(s) 44, 521
Tru9I TTAA 2 cut(s) 44, 521
TscAI CASTG 2 cut(s) 211, 480
TseI GCWGC 1 cut(s) 160
TspDTI ATGAA 3 cut(s) 169, 330, 362
TspRI CASTG 2 cut(s) 211, 480
VpaK11BI GGWCC 1 cut(s) 25
XapI RAATTY 1 cut(s) 227
XceI RCATGY 1 cut(s) 274
XcmI CCANNNNNNNNNTGG 1 cut(s) 19
XspI CTAG 2 cut(s) 455, 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.