MD17G1221200.v1.1

Mitochondrial glycoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007719
Physical Location & Seq
Forward (+)
26998252 .. 27000759
2508 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1221200.v1.1.491

Sequence Viewer

Length: 774 bp
ATGTTCCGAGGAGTCTCTTCCATGGCGCGCCTAATCCGACCCCTGCGAAAATCCACTCCTTTTCCTTCTTCTTCCAGAATCCCAATTCAGCAAACACCCACTTTGCATTTCGCACAAGAAAAACAAGTCTCCCTCTTAAACCCTTCACTCCAACCACCGCAATTCCGGCGAACTTACATCTCCGAAATGCGCAAATCGGCCTTCGAAGGCAACATTCTCAGACTCCTCCGGAGGGAAATACAGTACGAGCTCGATCACTCTCCTGCCACTCAGCCAGTTACAAAATTTAAGTCGTTTACCGTTAACGACCAGCCTGGTGAGCAATGGATAACCTTGAAGAGAAAGTTTGGAGAGAAAGAAGACATTAAGATCGAAGCCACGATGTTTGATGGATCTGTTCCTATTCCGAATTCCAAAGGTGGTGCTGGCCTTGGAAAAGATGTGCAGCTTCACATTACAATGATTGTTAATATCTCCAAGAAGGAAGGCGGCAATGTGTTTGAGATTATGTGTTCAGCTTGGCCAGATACTATAGAGATCAACAAGCTTTTTGTCCGAAAACCTGAGAAGATGCCAGCTCAGCCTTATGCGGGTCCTGAGTTCAAGGAACTTGATGATGAGTTGCAAGACTCACTTTATGAATTCTTGGAAGCAAGGAGTATAAACGATGATCTTGCTATTTTCTTGCACGAATACATGAAGAACAAAGATAAAACTGAGTTCATTAGATGGATGGGGACTGTGAAATCATTTATTGAAAAGAAAGTAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
MAM33

Protein Analysis

258

Amino Acids

29.64

Weight (kDa)

8.99

Isoelectric Point (pI)

61.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAM33 PF02330 76 - 252 1.2e-32 Mitochondrial glycoprotein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 191
AccII CGCG 1 cut(s) 28
AccIII TCCGGA 1 cut(s) 228
AciI CCGC 3 cut(s) 158, 489, 590
AclWI GGATC 1 cut(s) 400
AcoI YGGCCR 1 cut(s) 521
AcsI RAATTY 3 cut(s) 284, 409, 641
AfaI GTAC 1 cut(s) 245
AfiI CCNNNNNNNGG 2 cut(s) 232, 590
AgsI TTSAA 3 cut(s) 337, 604, 758
AjnI CCWGG 1 cut(s) 313
AluBI AGCT 5 cut(s) 250, 448, 518, 547, 578
AluI AGCT 5 cut(s) 250, 448, 518, 547, 578
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 2 cut(s) 19, 133
AlwI GGATC 1 cut(s) 400
Aor13HI TCCGGA 1 cut(s) 228
AoxI GGCC 3 cut(s) 198, 427, 521
ApeKI GCWGC 1 cut(s) 445
ApoI RAATTY 3 cut(s) 284, 409, 641
ArsI GACNNNNNNTTYG 2 cut(s) 275, 307
AscI GGCGCGCC 1 cut(s) 26
AspLEI GCGC 3 cut(s) 28, 30, 192
AspS9I GGNCC 1 cut(s) 593
AsuHPI GGTGA 1 cut(s) 329
AsuII TTCGAA 1 cut(s) 204
AvaII GGWCC 1 cut(s) 593
BalI TGGCCA 1 cut(s) 523
BanII GRGCYC 1 cut(s) 252
BbsI GAAGAC 1 cut(s) 366
Bbv12I GWGCWC 1 cut(s) 252
BbvI GCAGC 1 cut(s) 457
BccI CCATC 3 cut(s) 383, 723, 727
BcgI CGANNNNNNTGC 2 cut(s) 656, 690
BciT130I CCWGG 1 cut(s) 315
BcoDI GTCTC 2 cut(s) 19, 133
BfmI CTRYAG 1 cut(s) 531
BisI GCNGC 2 cut(s) 446, 490
BlpI GCTNAGC 1 cut(s) 579
BlsI GCNGC 2 cut(s) 447, 491
Bme1390I CCNGG 1 cut(s) 315
Bme18I GGWCC 1 cut(s) 593
BmgT120I GGNCC 1 cut(s) 593
BmiI GGNNCC 1 cut(s) 594
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 1 cut(s) 561
BpiI GAAGAC 1 cut(s) 366
Bpu1102I GCTNAGC 1 cut(s) 579
Bpu14I TTCGAA 1 cut(s) 204
BsaJI CCNNGG 3 cut(s) 7, 21, 430
BsaWI WCCGGW 1 cut(s) 228
Bsc4I CCNNNNNNNGG 2 cut(s) 232, 590
Bse1I ACTGG 1 cut(s) 275
Bse3DI GCAATG 2 cut(s) 329, 499
BseAI TCCGGA 1 cut(s) 228
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 3 cut(s) 7, 21, 430
BseGI GGATG 1 cut(s) 738
BseLI CCNNNNNNNGG 2 cut(s) 232, 590
BseMI GCAATG 2 cut(s) 329, 499
BseMII CTCAG 6 cut(s) 232, 284, 555, 588, 593, 708
BseNI ACTGG 1 cut(s) 275
BsePI GCGCGC 1 cut(s) 26
BseRI GAGGAG 2 cut(s) 24, 215
BseXI GCAGC 1 cut(s) 457
BsgI GTGCAG 1 cut(s) 464
Bsh1236I CGCG 1 cut(s) 28
BshFI GGCC 3 cut(s) 200, 429, 523
BsiHKAI GWGCWC 1 cut(s) 252
BsiSI CCGG 2 cut(s) 166, 229
BslFI GGGAC 1 cut(s) 751
BslI CCNNNNNNNGG 2 cut(s) 232, 590
BsmAI GTCTC 2 cut(s) 19, 133
BsmFI GGGAC 1 cut(s) 751
BsnI GGCC 3 cut(s) 200, 429, 523
Bsp119I TTCGAA 1 cut(s) 204
Bsp1286I GDGCHC 1 cut(s) 252
Bsp13I TCCGGA 1 cut(s) 228
Bsp143I GATC 5 cut(s) 253, 369, 392, 537, 670
Bsp1720I GCTNAGC 1 cut(s) 579
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 3 cut(s) 158, 489, 590
BspANI GGCC 3 cut(s) 200, 429, 523
BspCNI CTCAG 6 cut(s) 231, 283, 556, 589, 592, 709
BspEI TCCGGA 1 cut(s) 228
BspFNI CGCG 1 cut(s) 28
BspLI GGNNCC 1 cut(s) 594
BspPI GGATC 1 cut(s) 400
BspT104I TTCGAA 1 cut(s) 204
BsrDI GCAATG 2 cut(s) 329, 499
BsrI ACTGG 1 cut(s) 275
BssECI CCNNGG 3 cut(s) 7, 21, 430
BssHII GCGCGC 1 cut(s) 26
BssMI GATC 5 cut(s) 253, 369, 392, 537, 670
BssT1I CCWWGG 2 cut(s) 21, 430
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 3 cut(s) 243, 301, 742
Bst6I CTCTTC 2 cut(s) 22, 332
BstBI TTCGAA 1 cut(s) 204
BstC8I GCNNGC 3 cut(s) 28, 427, 576
BstDEI CTNAG 6 cut(s) 218, 270, 564, 579, 597, 717
BstDSI CCRYGG 1 cut(s) 21
BstF5I GGATG 1 cut(s) 738
BstFNI CGCG 1 cut(s) 28
BstHHI GCGC 3 cut(s) 28, 30, 192
BstKTI GATC 5 cut(s) 256, 372, 395, 540, 673
BstMAI GTCTC 2 cut(s) 19, 133
BstMBI GATC 5 cut(s) 253, 369, 392, 537, 670
BstMWI GCNNNNNNNGC 3 cut(s) 166, 319, 580
BstNI CCWGG 1 cut(s) 315
BstSCI CCNGG 1 cut(s) 313
BstSFI CTRYAG 1 cut(s) 531
BstUI CGCG 1 cut(s) 28
BstV1I GCAGC 1 cut(s) 457
BstV2I GAAGAC 1 cut(s) 366
BstX2I RGATCY 1 cut(s) 392
BstYI RGATCY 1 cut(s) 392
BsuRI GGCC 3 cut(s) 200, 429, 523
BtgI CCRYGG 1 cut(s) 21
BtsCI GGATG 1 cut(s) 738
Cac8I GCNNGC 3 cut(s) 28, 427, 576
CfoI GCGC 3 cut(s) 28, 30, 192
Cfr13I GGNCC 1 cut(s) 593
Csp6I GTAC 1 cut(s) 244
CspCI CAANNNNNGTGG 2 cut(s) 367, 402
CviAII CATG 2 cut(s) 22, 697
CviQI GTAC 1 cut(s) 244
DdeI CTNAG 6 cut(s) 218, 270, 564, 579, 597, 717
DpnI GATC 5 cut(s) 255, 371, 394, 539, 672
DpnII GATC 5 cut(s) 253, 369, 392, 537, 670
EaeI YGGCCR 1 cut(s) 521
Eam1104I CTCTTC 2 cut(s) 22, 332
EarI CTCTTC 2 cut(s) 22, 332
Ecl136II GAGCTC 1 cut(s) 250
Eco130I CCWWGG 2 cut(s) 21, 430
Eco24I GRGCYC 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 593
Eco53kI GAGCTC 1 cut(s) 250
EcoICRI GAGCTC 1 cut(s) 250
EcoO109I RGGNCCY 1 cut(s) 593
EcoRI GAATTC 2 cut(s) 409, 641
EcoRII CCWGG 1 cut(s) 313
EcoT14I CCWWGG 2 cut(s) 21, 430
EcoT38I GRGCYC 1 cut(s) 252
ErhI CCWWGG 2 cut(s) 21, 430
FaeI CATG 2 cut(s) 25, 700
FaiI YATR 7 cut(s) 23, 509, 533, 588, 639, 662, 698
FalI AAGNNNNNCTT 2 cut(s) 618, 650
FaqI GGGAC 1 cut(s) 751
FatI CATG 2 cut(s) 21, 696
FauI CCCGC 1 cut(s) 583
Fnu4HI GCNGC 2 cut(s) 446, 490
FokI GGATG 1 cut(s) 745
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 2 cut(s) 446, 490
FspI TGCGCA 1 cut(s) 191
GlaI GCGC 3 cut(s) 27, 29, 191
GluI GCNGC 2 cut(s) 446, 490
HaeIII GGCC 3 cut(s) 200, 429, 523
HapII CCGG 2 cut(s) 166, 229
HhaI GCGC 3 cut(s) 28, 30, 192
Hin1II CATG 2 cut(s) 25, 700
Hin6I GCGC 3 cut(s) 26, 28, 190
HinP1I GCGC 3 cut(s) 26, 28, 190
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HindIII AAGCTT 1 cut(s) 545
HinfI GANTC 4 cut(s) 12, 78, 222, 629
HpaI GTTAAC 1 cut(s) 304
HpaII CCGG 2 cut(s) 166, 229
HphI GGTGA 1 cut(s) 329
Hpy166II GTNNAC 2 cut(s) 297, 304
Hpy188I TCNGA 6 cut(s) 8, 38, 184, 221, 408, 557
Hpy188III TCNNGA 3 cut(s) 75, 229, 596
Hpy8I GTNNAC 2 cut(s) 297, 304
HpyAV CCTTC 6 cut(s) 75, 153, 200, 211, 475, 479
HpyCH4III ACNGT 3 cut(s) 243, 301, 742
HpyCH4V TGCA 4 cut(s) 106, 445, 625, 688
HpyF10VI GCNNNNNNNGC 3 cut(s) 166, 319, 580
HpyF3I CTNAG 6 cut(s) 218, 270, 564, 579, 597, 717
Hsp92II CATG 2 cut(s) 25, 700
HspAI GCGC 3 cut(s) 26, 28, 190
Kpn2I TCCGGA 1 cut(s) 228
KspAI GTTAAC 1 cut(s) 304
Kzo9I GATC 5 cut(s) 253, 369, 392, 537, 670
Lsp1109I GCAGC 1 cut(s) 457
LweI GCATC 1 cut(s) 561
MaeIII GTNAC 1 cut(s) 277
MalI GATC 5 cut(s) 255, 371, 394, 539, 672
MboI GATC 5 cut(s) 253, 369, 392, 537, 670
MboII GAAGA 7 cut(s) 9, 60, 63, 349, 371, 580, 712
MflI RGATCY 1 cut(s) 392
MhlI GDGCHC 1 cut(s) 252
MlsI TGGCCA 1 cut(s) 523
MluCI AATT 5 cut(s) 84, 161, 284, 409, 641
MluNI TGGCCA 1 cut(s) 523
MlyI GAGTC 3 cut(s) 21, 216, 623
MmeI TCCRAC 2 cut(s) 61, 175
MnlI CCTC 3 cut(s) 143, 225, 236
Mox20I TGGCCA 1 cut(s) 523
MroI TCCGGA 1 cut(s) 228
MscI TGGCCA 1 cut(s) 523
MseI TTAA 5 cut(s) 137, 288, 303, 366, 468
MslI CAYNNNNRTG 1 cut(s) 458
Msp20I TGGCCA 1 cut(s) 523
MspI CCGG 2 cut(s) 166, 229
MspR9I CCNGG 1 cut(s) 315
MvaI CCWGG 1 cut(s) 315
MvnI CGCG 1 cut(s) 28
MwoI GCNNNNNNNGC 3 cut(s) 166, 319, 580
NcoI CCATGG 1 cut(s) 21
NdeII GATC 5 cut(s) 253, 369, 392, 537, 670
NlaIII CATG 2 cut(s) 25, 700
NlaIV GGNNCC 1 cut(s) 594
NsbI TGCGCA 1 cut(s) 191
NspV TTCGAA 1 cut(s) 204
PalAI GGCGCGCC 1 cut(s) 26
PauI GCGCGC 1 cut(s) 26
PfeI GAWTC 1 cut(s) 78
PkrI GCNGC 2 cut(s) 447, 491
PleI GAGTC 3 cut(s) 20, 216, 623
PpsI GAGTC 3 cut(s) 20, 216, 623
PpuMI RGGWCCY 1 cut(s) 593
Psp124BI GAGCTC 1 cut(s) 252
Psp5II RGGWCCY 1 cut(s) 593
Psp6I CCWGG 1 cut(s) 313
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 1 cut(s) 594
PspPI GGNCC 1 cut(s) 593
PspPPI RGGWCCY 1 cut(s) 593
PsuI RGATCY 1 cut(s) 392
PteI GCGCGC 1 cut(s) 26
RsaI GTAC 1 cut(s) 245
RsaNI GTAC 1 cut(s) 244
RseI CAYNNNNRTG 1 cut(s) 458
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 5 cut(s) 137, 288, 303, 366, 468
SatI GCNGC 2 cut(s) 446, 490
Sau3AI GATC 5 cut(s) 253, 369, 392, 537, 670
Sau96I GGNCC 1 cut(s) 593
SchI GAGTC 3 cut(s) 21, 216, 623
ScrFI CCNGG 1 cut(s) 315
SduI GDGCHC 1 cut(s) 252
SetI ASST 8 cut(s) 252, 335, 421, 450, 520, 549, 565, 580
SfaNI GCATC 1 cut(s) 561
SfcI CTRYAG 1 cut(s) 531
SfuI TTCGAA 1 cut(s) 204
SgsI GGCGCGCC 1 cut(s) 26
SinI GGWCC 1 cut(s) 593
SmiMI CAYNNNNRTG 1 cut(s) 458
Sse9I AATT 5 cut(s) 84, 161, 284, 409, 641
SsiI CCGC 3 cut(s) 158, 489, 590
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 1 cut(s) 313
StyI CCWWGG 2 cut(s) 21, 430
TaaI ACNGT 3 cut(s) 243, 301, 742
TaqI TCGA 3 cut(s) 204, 252, 372
TasI AATT 5 cut(s) 84, 161, 284, 409, 641
TauI GCSGC 1 cut(s) 492
TfiI GAWTC 1 cut(s) 78
Tru1I TTAA 5 cut(s) 137, 288, 303, 366, 468
Tru9I TTAA 5 cut(s) 137, 288, 303, 366, 468
TseI GCWGC 1 cut(s) 445
TspDTI ATGAA 3 cut(s) 654, 712, 713
VpaK11BI GGWCC 1 cut(s) 593
XapI RAATTY 3 cut(s) 284, 409, 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.