Prupe.1G178200_v2.0.a1

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0015539
Physical Location & Seq
Reverse (-)
15284702 .. 15286472
1771 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G178200.1

Sequence Viewer

Length: 1032 bp
ATGCGGGATTTTGGCATGGCTCTTGAGGATTGTGGTTTGACATCGATGAAGTATAAGGGTTACAAATTTACTTGGACAAACAATCGAACTGGGGATGACAGAGTCCAACTTCGGCTGGATAGGGGGTGGCGAATGTTGCATTCGGTGCAGTGGGCTCGAAGATTTATGTTTGAAGAAATGTGGACTGAAGTTGATGGGTGTACGGAGACAGTTGTTCAAGCTTGGGGAAAGGAACAAGGTGATCTGGTTACTAAGTTAAAAGCTTGCCAACAGTCCTTACTAGATTGGAATAAATCCAAGGTGGGCCATATTCCCATGCAGATACAACAGATTCAACGGCAATTTGATGGATTCAAAAAGAAACCGCAAACGGATGATGTGGTGCGGTGTCAACGTAGGCTGGCTGGTGAGTTAGATATACTACTTGCAAGGGAGGAAATGATCTGGCAACAAAGGTCTCGAGTCTCTTGGCTTAAATATGAGGATCATAACACAAAATTCTTTCATGCACAAGCTAAACAAAGGGGACGAAGAAATTTCATGCAAGGTATTTTTTATGAGCATAATGTGTGGAATACTTCTAATGAAGGCGTTGGTTCTATTTTTTGTGAGTATTTCAAGAAGTATTCACCACTAGTGGTGAGAATGATTCTAGCACTGTACTGGCATGTACGAGAGATCAACCATACTCTCGTCACTCTTATCCCAAAGGTGGACAATCCCACACGAGTTACTGAGTACCGGCCAATAAGTTTATGCTCGGTTCTCTATAAACTGATATCAAAAACACTGGTGAACAGAATGAAGGGTATTATGCAGGATGTCATCTCAGAGTACCAAAGCGCCTTTGTTCCTTCTCAGTTGATTACTGACAACATTATTGCCGCTTTCGAAAGCATTCACGCCATCCAAAGAAGGAGGGGAAGTGCTCTGAAAAAGATGATATTGAAGCTTGATATTATTAAGCTTGATACTAATTCAACATGGTATCAGAGCTCCTGGTTTGATTCCAGAGAGGGGCGTGAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

344

Amino Acids

40.6

Weight (kDa)

9.5

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 4, 365, 385, 885
AclWI GGATC 1 cut(s) 492
AcoI YGGCCR 1 cut(s) 743
AcsI RAATTY 3 cut(s) 65, 497, 535
AcuI CTGAAG 1 cut(s) 207
AfaI GTAC 5 cut(s) 202, 662, 672, 740, 836
AfiI CCNNNNNNNGG 2 cut(s) 712, 1017
AgsI TTSAA 7 cut(s) 173, 218, 335, 355, 619, 949, 981
AhlI ACTAGT 1 cut(s) 634
AjnI CCWGG 1 cut(s) 998
AluBI AGCT 7 cut(s) 221, 263, 515, 952, 967, 996, 1027
AluI AGCT 7 cut(s) 221, 263, 515, 952, 967, 996, 1027
Alw21I GWGCWC 2 cut(s) 931, 998
Alw26I GTCTC 3 cut(s) 200, 462, 469
AlwI GGATC 1 cut(s) 492
Ama87I CYCGRG 1 cut(s) 459
AoxI GGCC 2 cut(s) 304, 743
ApoI RAATTY 3 cut(s) 65, 497, 535
Asp700I GAANNNNTTC 1 cut(s) 897
AspLEI GCGC 1 cut(s) 845
AspS9I GGNCC 1 cut(s) 304
AsuHPI GGTGA 5 cut(s) 251, 419, 621, 652, 805
AsuII TTCGAA 1 cut(s) 891
AvaI CYCGRG 1 cut(s) 459
BanII GRGCYC 2 cut(s) 157, 998
BauI CACGAG 1 cut(s) 726
Bbv12I GWGCWC 2 cut(s) 931, 998
BccI CCATC 3 cut(s) 188, 341, 914
BceAI ACGGC 1 cut(s) 353
BcgI CGANNNNNNTGC 2 cut(s) 137, 171
BciT130I CCWGG 1 cut(s) 1000
BcoDI GTCTC 3 cut(s) 200, 462, 469
BcuI ACTAGT 1 cut(s) 634
BfaI CTAG 3 cut(s) 281, 635, 653
BfoI RGCGCY 1 cut(s) 846
BisI GCNGC 1 cut(s) 885
BlsI GCNGC 1 cut(s) 886
Bme1390I CCNGG 1 cut(s) 1000
BmeT110I CYCGRG 1 cut(s) 459
BmgT120I GGNCC 1 cut(s) 304
BmrFI CCNGG 1 cut(s) 1000
BmrI ACTGGG 1 cut(s) 99
BmuI ACTGGG 1 cut(s) 99
Bpu14I TTCGAA 1 cut(s) 891
BpuEI CTTGAG 1 cut(s) 44
Bsa29I ATCGAT 1 cut(s) 44
BsaI GGTCTC 1 cut(s) 462
BsaJI CCNNGG 1 cut(s) 297
BsaXI ACNNNNNCTCC 2 cut(s) 910, 940
Bsc4I CCNNNNNNNGG 2 cut(s) 712, 1017
Bse118I RCCGGY 1 cut(s) 741
Bse1I ACTGG 3 cut(s) 94, 668, 795
BseBI CCWGG 1 cut(s) 1000
BseCI ATCGAT 1 cut(s) 44
BseDI CCNNGG 1 cut(s) 297
BseGI GGATG 4 cut(s) 100, 379, 826, 906
BseLI CCNNNNNNNGG 2 cut(s) 712, 1017
BseMII CTCAG 3 cut(s) 726, 843, 872
BseNI ACTGG 3 cut(s) 94, 668, 795
BsgI GTGCAG 1 cut(s) 167
BshFI GGCC 2 cut(s) 306, 745
BshVI ATCGAT 1 cut(s) 44
BsiHKAI GWGCWC 2 cut(s) 931, 998
BsiHKCI CYCGRG 1 cut(s) 459
BsiSI CCGG 1 cut(s) 742
BslFI GGGAC 1 cut(s) 540
BslI CCNNNNNNNGG 2 cut(s) 712, 1017
BsmAI GTCTC 3 cut(s) 200, 462, 469
BsmFI GGGAC 1 cut(s) 540
BsmI GAATGC 2 cut(s) 139, 897
BsnI GGCC 2 cut(s) 306, 745
Bso31I GGTCTC 1 cut(s) 462
BsoBI CYCGRG 1 cut(s) 459
Bsp119I TTCGAA 1 cut(s) 891
Bsp1286I GDGCHC 3 cut(s) 157, 931, 998
Bsp143I GATC 4 cut(s) 241, 441, 484, 678
BspACI CCGC 4 cut(s) 4, 365, 385, 885
BspANI GGCC 2 cut(s) 306, 745
BspCNI CTCAG 3 cut(s) 727, 842, 871
BspDI ATCGAT 1 cut(s) 44
BspPI GGATC 1 cut(s) 492
BspT104I TTCGAA 1 cut(s) 891
BspTNI GGTCTC 1 cut(s) 462
BsrFI RCCGGY 1 cut(s) 741
BsrI ACTGG 3 cut(s) 94, 668, 795
BssAI RCCGGY 1 cut(s) 741
BssECI CCNNGG 1 cut(s) 297
BssMI GATC 4 cut(s) 241, 441, 484, 678
BssSI CACGAG 1 cut(s) 726
BssT1I CCWWGG 1 cut(s) 297
Bst2BI CACGAG 1 cut(s) 726
Bst2UI CCWGG 1 cut(s) 1000
Bst4CI ACNGT 3 cut(s) 211, 273, 660
BstAPI GCANNNNNTGC 1 cut(s) 145
BstBI TTCGAA 1 cut(s) 891
BstC8I GCNNGC 2 cut(s) 265, 402
BstDEI CTNAG 4 cut(s) 252, 735, 829, 858
BstF5I GGATG 4 cut(s) 100, 379, 826, 906
BstH2I RGCGCY 1 cut(s) 846
BstHHI GCGC 1 cut(s) 845
BstKTI GATC 4 cut(s) 244, 444, 487, 681
BstMAI GTCTC 3 cut(s) 200, 462, 469
BstMBI GATC 4 cut(s) 241, 441, 484, 678
BstMWI GCNNNNNNNGC 2 cut(s) 136, 145
BstNI CCWGG 1 cut(s) 1000
BstNSI RCATGY 1 cut(s) 671
BstSCI CCNGG 1 cut(s) 998
Bsu15I ATCGAT 1 cut(s) 44
BsuRI GGCC 2 cut(s) 306, 745
BsuTUI ATCGAT 1 cut(s) 44
BtsCI GGATG 4 cut(s) 100, 379, 826, 906
BtsI GCAGTG 1 cut(s) 155
BtsIMutI CAGTG 3 cut(s) 155, 656, 788
Cac8I GCNNGC 2 cut(s) 265, 402
CfoI GCGC 1 cut(s) 845
Cfr10I RCCGGY 1 cut(s) 741
Cfr13I GGNCC 1 cut(s) 304
ClaI ATCGAT 1 cut(s) 44
Csp6I GTAC 5 cut(s) 201, 661, 671, 739, 835
CviAII CATG 6 cut(s) 16, 316, 506, 541, 668, 984
CviQI GTAC 5 cut(s) 201, 661, 671, 739, 835
DdeI CTNAG 4 cut(s) 252, 735, 829, 858
DpnI GATC 4 cut(s) 243, 443, 486, 680
DpnII GATC 4 cut(s) 241, 441, 484, 678
EaeI YGGCCR 1 cut(s) 743
Ecl136II GAGCTC 1 cut(s) 996
Eco130I CCWWGG 1 cut(s) 297
Eco24I GRGCYC 2 cut(s) 157, 998
Eco31I GGTCTC 1 cut(s) 462
Eco32I GATATC 1 cut(s) 780
Eco53kI GAGCTC 1 cut(s) 996
Eco57I CTGAAG 1 cut(s) 207
Eco88I CYCGRG 1 cut(s) 459
EcoICRI GAGCTC 1 cut(s) 996
EcoRII CCWGG 1 cut(s) 998
EcoRV GATATC 1 cut(s) 780
EcoT14I CCWWGG 1 cut(s) 297
EcoT38I GRGCYC 2 cut(s) 157, 998
ErhI CCWWGG 1 cut(s) 297
FaeI CATG 6 cut(s) 19, 319, 509, 544, 671, 987
FaqI GGGAC 1 cut(s) 540
FatI CATG 6 cut(s) 15, 315, 505, 540, 667, 983
Fnu4HI GCNGC 1 cut(s) 885
FokI GGATG 4 cut(s) 107, 386, 833, 893
FriOI GRGCYC 2 cut(s) 157, 998
Fsp4HI GCNGC 1 cut(s) 885
FspBI CTAG 3 cut(s) 281, 635, 653
GlaI GCGC 1 cut(s) 844
GluI GCNGC 1 cut(s) 885
HaeII RGCGCY 1 cut(s) 846
HaeIII GGCC 2 cut(s) 306, 745
HapII CCGG 1 cut(s) 742
HhaI GCGC 1 cut(s) 845
Hin1II CATG 6 cut(s) 19, 319, 509, 544, 671, 987
Hin6I GCGC 1 cut(s) 843
HinP1I GCGC 1 cut(s) 843
HincII GTYRAC 1 cut(s) 392
HindII GTYRAC 1 cut(s) 392
HindIII AAGCTT 4 cut(s) 219, 261, 950, 965
HinfI GANTC 6 cut(s) 102, 331, 351, 462, 649, 1007
HpaII CCGG 1 cut(s) 742
HphI GGTGA 5 cut(s) 251, 419, 621, 652, 805
Hpy166II GTNNAC 5 cut(s) 183, 201, 392, 715, 796
Hpy188I TCNGA 3 cut(s) 832, 933, 993
Hpy188III TCNNGA 4 cut(s) 23, 459, 619, 1011
Hpy8I GTNNAC 5 cut(s) 183, 201, 392, 715, 796
HpyAV CCTTC 4 cut(s) 581, 799, 864, 909
HpyCH4III ACNGT 3 cut(s) 211, 273, 660
HpyCH4IV ACGT 1 cut(s) 394
HpyCH4V TGCA 7 cut(s) 139, 148, 319, 428, 509, 544, 817
HpyF10VI GCNNNNNNNGC 2 cut(s) 136, 145
HpyF3I CTNAG 4 cut(s) 252, 735, 829, 858
HpySE526I ACGT 1 cut(s) 394
Hsp92II CATG 6 cut(s) 19, 319, 509, 544, 671, 987
HspAI GCGC 1 cut(s) 843
Kzo9I GATC 4 cut(s) 241, 441, 484, 678
LmnI GCTCC 1 cut(s) 1001
MaeI CTAG 3 cut(s) 281, 635, 653
MaeII ACGT 1 cut(s) 394
MaeIII GTNAC 4 cut(s) 59, 247, 694, 730
MalI GATC 4 cut(s) 243, 443, 486, 680
MboI GATC 4 cut(s) 241, 441, 484, 678
MboII GAAGA 3 cut(s) 171, 185, 543
MhlI GDGCHC 3 cut(s) 157, 931, 998
MluCI AATT 5 cut(s) 65, 341, 497, 535, 976
MlyI GAGTC 2 cut(s) 111, 471
MmeI TCCRAC 1 cut(s) 130
MnlI CCTC 5 cut(s) 19, 427, 475, 912, 1009
MroXI GAANNNNTTC 1 cut(s) 897
MseI TTAA 3 cut(s) 257, 474, 963
MspI CCGG 1 cut(s) 742
MspR9I CCNGG 1 cut(s) 1000
Mva1269I GAATGC 2 cut(s) 139, 897
MvaI CCWGG 1 cut(s) 1000
MwoI GCNNNNNNNGC 2 cut(s) 136, 145
NdeII GATC 4 cut(s) 241, 441, 484, 678
NlaIII CATG 6 cut(s) 19, 319, 509, 544, 671, 987
NmuCI GTSAC 1 cut(s) 694
NspI RCATGY 1 cut(s) 671
NspV TTCGAA 1 cut(s) 891
PaeR7I CTCGAG 1 cut(s) 459
PctI GAATGC 2 cut(s) 139, 897
PdmI GAANNNNTTC 1 cut(s) 897
PfeI GAWTC 4 cut(s) 331, 351, 649, 1007
PflFI GACNNNGTC 1 cut(s) 101
PkrI GCNGC 1 cut(s) 886
PleI GAGTC 2 cut(s) 110, 470
PpsI GAGTC 2 cut(s) 110, 470
Psp124BI GAGCTC 1 cut(s) 998
Psp6I CCWGG 1 cut(s) 998
PspGI CCWGG 1 cut(s) 998
PspPI GGNCC 1 cut(s) 304
PsyI GACNNNGTC 1 cut(s) 101
RsaI GTAC 5 cut(s) 202, 662, 672, 740, 836
RsaNI GTAC 5 cut(s) 201, 661, 671, 739, 835
SacI GAGCTC 1 cut(s) 998
SaqAI TTAA 3 cut(s) 257, 474, 963
SatI GCNGC 1 cut(s) 885
Sau3AI GATC 4 cut(s) 241, 441, 484, 678
Sau96I GGNCC 1 cut(s) 304
SchI GAGTC 2 cut(s) 111, 471
ScrFI CCNGG 1 cut(s) 1000
SduI GDGCHC 3 cut(s) 157, 931, 998
Sfr274I CTCGAG 1 cut(s) 459
SfuI TTCGAA 1 cut(s) 891
SlaI CTCGAG 1 cut(s) 459
SmlI CTYRAG 2 cut(s) 23, 459
SmoI CTYRAG 2 cut(s) 23, 459
SpeI ACTAGT 1 cut(s) 634
Sse9I AATT 5 cut(s) 65, 341, 497, 535, 976
SsiI CCGC 4 cut(s) 4, 365, 385, 885
SspMI CTAG 3 cut(s) 281, 635, 653
SstI GAGCTC 1 cut(s) 998
StyD4I CCNGG 1 cut(s) 998
StyI CCWWGG 1 cut(s) 297
TaaI ACNGT 3 cut(s) 211, 273, 660
TaiI ACGT 1 cut(s) 397
TaqI TCGA 5 cut(s) 44, 85, 157, 460, 891
TasI AATT 5 cut(s) 65, 341, 497, 535, 976
TatI WGTACW 1 cut(s) 660
TauI GCSGC 1 cut(s) 887
TfiI GAWTC 4 cut(s) 331, 351, 649, 1007
Tru1I TTAA 3 cut(s) 257, 474, 963
Tru9I TTAA 3 cut(s) 257, 474, 963
TscAI CASTG 3 cut(s) 155, 663, 795
TseFI GTSAC 1 cut(s) 694
Tsp45I GTSAC 1 cut(s) 694
TspDTI ATGAA 5 cut(s) 62, 494, 529, 600, 818
TspGWI ACGGA 2 cut(s) 218, 386
TspRI CASTG 3 cut(s) 155, 663, 795
Tth111I GACNNNGTC 1 cut(s) 101
XapI RAATTY 3 cut(s) 65, 497, 535
XceI RCATGY 1 cut(s) 671
XhoI CTCGAG 1 cut(s) 459
XmnI GAANNNNTTC 1 cut(s) 897
XspI CTAG 3 cut(s) 281, 635, 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.