Rw1G005070

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0015539
Physical Location & Seq
Forward (+)
9722423 .. 9726921
4499 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G005070.1

Sequence Viewer

Length: 1476 bp
ATGTCTGAGAAGGGAGGAGGTGCTAGACCCGAAGCTCAAATGCGTAGATTTCGGGAGGTTATTAATGATTGCGTTCTACAGGATGTTGAGTTTTCTGGACCACCCACTACTTGGAAACATGGTGACACTCTCGAAAGGCTAGATAGATGCCTAGTGAACCAGAGGGCAGCAGATCTTTTTCCGAATTTGCATGAGCTTCATTTGGATGTTAGTGCTTCTGATCATCTAGCACTATTGATATATTCTAATAGGAGACACTTGGAACAGGAGAGTCAAACAAGATTTCACCGAAGATTCCAATTTGAGGCGTTTTGGACTAAGGAAGAACAGTGTGAGAAGATTATTTTGGAGCAGTGGTTGCAGGATTCTACACAGATAGGGGTTGTTACTAAGCTGCAGAGAGTTGCTCAAGCTCTGCAAAGTTGGAATAAGAATACGGTGGGTCACATTCCGAGAAAGATTAAGATGTTGAATGCCAAGCTTCAACAGTACCCATTTGATAATTTGGAGGAGGGAGTACAGGAGAGTAGGAAGCTAGTGCTATCTGATCCAAGGAAATATATGGACCATGAAGAGCTGATGTGGAAGCAAAAATCAAGAGTCTGTTGGTTACAGGACGGAGATCAAAATACAAAGTTTCTTCATGTCTTTGCTAAAGCAAGGGGAAGATGTAATAAGATTTTGGGGGTATCGGATTTGAATGGGAACTGCCAGGAGAGTGCTGCAGGGGTTCAACAAGCTTTCATTGGCTATTTTGAACAACTTTTTGCCACCGAAGGTTGTAATCACATGGAGTTGGTGTTGGATTCTATTCAACGTAAAGTCACTGATACCATGAATTGTAAGCTTGCACAGCCTTTCACTCAGTTAGAAATTGAGACAGCGTTGAAGCAAATGCCGCCGGACAAGGCACCGGGTGAAGATGGTTTTTCGGCCCAGTTTTATCAAAAGTACTGGCAAGTGATTGGGGATGATATCAGCCGGGATTGTTTGAAGTGTCTAAATGAAGGTGCAGATATGATTGTTTTTGATCATACTCTCTTTGCTCTGATTCCTAAGGTGGATGTTCCACAGACACTAACTGATTTCAGACCAATTGGTCTGTGTAACGTTATTTATAAATTGATGTCTAAGGCTATTGTCAACAGAATGAAGTCGACTCTTCCTGGGGTAATATCCCCGTTCCAGAGTGCCTTTGTTCCTGGCAGATGCATTCATGATAATATTATCGCTGCTTTTGAAACGGTTCATTCTATTAGAGCTAAGTTGGTGGAGGGTGGGTCGAAATGTGTTTTGAAATTGGATATAAACAACGCTTACGATAGGGTGGAATGGATGTCTTTATGTCAGATAATACTTTGGTTGGGGTTTATGGAAGGTGGGTTGAGCTCATTATGCATTGTGTTACTTCGGTTTCCTTCAGTGTGTTATGGAATGGCCAAGCTGCAGGCTGGATTAAGCCAACTAGGGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

491

Amino Acids

55.94

Weight (kDa)

6.89

Isoelectric Point (pI)

46.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 351 - 452 7.2e-14 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1119
AasI GACNNNNNNGTC 1 cut(s) 1098
AccB1I GGYRCC 1 cut(s) 910
AccB7I CCANNNNNTGG 1 cut(s) 111
AccI GTMKAC 1 cut(s) 1157
AciI CCGC 1 cut(s) 899
AclI AACGTT 1 cut(s) 1110
AclWI GGATC 1 cut(s) 542
AcoI YGGCCR 1 cut(s) 1437
AcsI RAATTY 1 cut(s) 184
AcuI CTGAAG 1 cut(s) 1404
AdeI CACNNNGTG 1 cut(s) 917
AfaI GTAC 3 cut(s) 491, 519, 953
AfiI CCNNNNNNNGG 3 cut(s) 111, 304, 1468
AjnI CCWGG 3 cut(s) 711, 1165, 1201
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
Alw21I GWGCWC 1 cut(s) 1391
Alw26I GTCTC 2 cut(s) 247, 872
AlwI GGATC 1 cut(s) 542
AoxI GGCC 2 cut(s) 933, 1437
ApeKI GCWGC 5 cut(s) 167, 394, 722, 1232, 1444
ApoI RAATTY 1 cut(s) 184
AseI ATTAAT 1 cut(s) 63
Asp700I GAANNNNTTC 1 cut(s) 1245
AspS9I GGNCC 3 cut(s) 98, 565, 934
AsuC2I CCSGG 2 cut(s) 915, 983
AsuHPI GGTGA 3 cut(s) 134, 278, 929
AvaII GGWCC 2 cut(s) 98, 565
AxyI CCTNAGG 1 cut(s) 1056
BalI TGGCCA 1 cut(s) 1439
BanI GGYRCC 1 cut(s) 910
BanII GRGCYC 1 cut(s) 1391
Bbv12I GWGCWC 1 cut(s) 1391
BbvI GCAGC 5 cut(s) 179, 381, 709, 1219, 1431
BccI CCATC 1 cut(s) 917
BciT130I CCWGG 3 cut(s) 713, 1167, 1203
BclI TGATCA 2 cut(s) 220, 1030
BcnI CCSGG 2 cut(s) 915, 983
BcoDI GTCTC 2 cut(s) 247, 872
BfaI CTAG 6 cut(s) 24, 140, 152, 227, 536, 1466
BfmI CTRYAG 4 cut(s) 77, 395, 723, 1445
BglII AGATCT 1 cut(s) 172
BisI GCNGC 6 cut(s) 168, 395, 723, 899, 1233, 1445
BlsI GCNGC 6 cut(s) 169, 396, 724, 900, 1234, 1446
BmcAI AGTACT 1 cut(s) 953
Bme1390I CCNGG 5 cut(s) 713, 915, 983, 1167, 1203
Bme18I GGWCC 2 cut(s) 98, 565
BmgT120I GGNCC 3 cut(s) 98, 565, 934
BmiI GGNNCC 1 cut(s) 912
BmrFI CCNGG 5 cut(s) 713, 915, 983, 1167, 1203
BmrI ACTGGG 1 cut(s) 931
BmsI GCATC 2 cut(s) 137, 1199
BmuI ACTGGG 1 cut(s) 931
BplI GAGNNNNNCTC 2 cut(s) 391, 423
BpuEI CTTGAG 1 cut(s) 393
BpuMI CCSGG 2 cut(s) 915, 983
BsaJI CCNNGG 2 cut(s) 551, 1166
BsaXI ACNNNNNCTCC 6 cut(s) 341, 371, 785, 815, 1265, 1295
Bsc4I CCNNNNNNNGG 3 cut(s) 111, 304, 1468
Bse1I ACTGG 2 cut(s) 937, 959
Bse21I CCTNAGG 1 cut(s) 1056
BseBI CCWGG 3 cut(s) 713, 1167, 1203
BseDI CCNNGG 2 cut(s) 551, 1166
BseGI GGATG 5 cut(s) 88, 211, 976, 1069, 1341
BseLI CCNNNNNNNGG 3 cut(s) 111, 304, 1468
BseMII CTCAG 1 cut(s) 878
BseNI ACTGG 2 cut(s) 937, 959
BseRI GAGGAG 2 cut(s) 30, 524
BseXI GCAGC 5 cut(s) 179, 381, 709, 1219, 1431
BsgI GTGCAG 1 cut(s) 1032
BshFI GGCC 2 cut(s) 935, 1439
BshNI GGYRCC 1 cut(s) 910
BsiHKAI GWGCWC 1 cut(s) 1391
BsiSI CCGG 3 cut(s) 902, 914, 982
BslI CCNNNNNNNGG 3 cut(s) 111, 304, 1468
BsmAI GTCTC 2 cut(s) 247, 872
BsmI GAATGC 2 cut(s) 478, 1212
BsnI GGCC 2 cut(s) 935, 1439
Bsp1286I GDGCHC 1 cut(s) 1391
Bsp143I GATC 5 cut(s) 172, 220, 547, 622, 1030
BspACI CCGC 1 cut(s) 899
BspANI GGCC 2 cut(s) 935, 1439
BspCNI CTCAG 1 cut(s) 877
BspHI TCATGA 1 cut(s) 1216
BspLI GGNNCC 1 cut(s) 912
BspMAI CTGCAG 3 cut(s) 399, 727, 1449
BspPI GGATC 1 cut(s) 542
BspQI GCTCTTC 1 cut(s) 567
BspT107I GGYRCC 1 cut(s) 910
BsrI ACTGG 2 cut(s) 937, 959
BssECI CCNNGG 2 cut(s) 551, 1166
BssMI GATC 5 cut(s) 172, 220, 547, 622, 1030
BssT1I CCWWGG 1 cut(s) 551
Bst2UI CCWGG 3 cut(s) 713, 1167, 1203
Bst4CI ACNGT 4 cut(s) 330, 439, 489, 1246
Bst6I CTCTTC 2 cut(s) 567, 1167
BstAPI GCANNNNNTGC 1 cut(s) 358
BstC8I GCNNGC 2 cut(s) 849, 1449
BstDEI CTNAG 7 cut(s) 6, 318, 390, 864, 1056, 1131, 1263
BstF5I GGATG 5 cut(s) 88, 211, 976, 1069, 1341
BstKTI GATC 5 cut(s) 175, 223, 550, 625, 1033
BstMAI GTCTC 2 cut(s) 247, 872
BstMBI GATC 5 cut(s) 172, 220, 547, 622, 1030
BstMWI GCNNNNNNNGC 4 cut(s) 358, 853, 898, 1395
BstNI CCWGG 3 cut(s) 713, 1167, 1203
BstSCI CCNGG 5 cut(s) 711, 913, 981, 1165, 1201
BstSFI CTRYAG 4 cut(s) 77, 395, 723, 1445
BstV1I GCAGC 5 cut(s) 179, 381, 709, 1219, 1431
BstX2I RGATCY 1 cut(s) 172
BstYI RGATCY 1 cut(s) 172
Bsu36I CCTNAGG 1 cut(s) 1056
BsuRI GGCC 2 cut(s) 935, 1439
BtsCI GGATG 5 cut(s) 88, 211, 976, 1069, 1341
BtsI GCAGTG 1 cut(s) 359
BtsIMutI CAGTG 4 cut(s) 335, 359, 825, 1428
Cac8I GCNNGC 2 cut(s) 849, 1449
CciI TCATGA 1 cut(s) 1216
Cfr13I GGNCC 3 cut(s) 98, 565, 934
Csp6I GTAC 3 cut(s) 490, 518, 952
CviAII CATG 7 cut(s) 119, 191, 569, 644, 790, 835, 1217
CviQI GTAC 3 cut(s) 490, 518, 952
DdeI CTNAG 7 cut(s) 6, 318, 390, 864, 1056, 1131, 1263
DpnI GATC 5 cut(s) 174, 222, 549, 624, 1032
DpnII GATC 5 cut(s) 172, 220, 547, 622, 1030
DraIII CACNNNGTG 1 cut(s) 917
DrdI GACNNNNNNGTC 1 cut(s) 1098
DseDI GACNNNNNNGTC 1 cut(s) 1098
EaeI YGGCCR 1 cut(s) 1437
Eam1104I CTCTTC 2 cut(s) 567, 1167
EarI CTCTTC 2 cut(s) 567, 1167
Ecl136II GAGCTC 1 cut(s) 1389
Eco130I CCWWGG 1 cut(s) 551
Eco24I GRGCYC 1 cut(s) 1391
Eco32I GATATC 1 cut(s) 976
Eco47I GGWCC 2 cut(s) 98, 565
Eco53kI GAGCTC 1 cut(s) 1389
Eco57I CTGAAG 1 cut(s) 1404
Eco81I CCTNAGG 1 cut(s) 1056
EcoICRI GAGCTC 1 cut(s) 1389
EcoRII CCWGG 3 cut(s) 711, 1165, 1201
EcoRV GATATC 1 cut(s) 976
EcoT14I CCWWGG 1 cut(s) 551
EcoT22I ATGCAT 2 cut(s) 1214, 1400
EcoT38I GRGCYC 1 cut(s) 1391
ErhI CCWWGG 1 cut(s) 551
FaeI CATG 7 cut(s) 122, 194, 572, 647, 793, 838, 1220
FatI CATG 7 cut(s) 118, 190, 568, 643, 789, 834, 1216
FbaI TGATCA 2 cut(s) 220, 1030
FblI GTMKAC 1 cut(s) 1157
Fnu4HI GCNGC 6 cut(s) 168, 395, 723, 899, 1233, 1445
FokI GGATG 5 cut(s) 95, 218, 983, 1076, 1348
FriOI GRGCYC 1 cut(s) 1391
Fsp4HI GCNGC 6 cut(s) 168, 395, 723, 899, 1233, 1445
FspBI CTAG 6 cut(s) 24, 140, 152, 227, 536, 1466
GluI GCNGC 6 cut(s) 168, 395, 723, 899, 1233, 1445
HaeIII GGCC 2 cut(s) 935, 1439
HapII CCGG 3 cut(s) 902, 914, 982
Hin1II CATG 7 cut(s) 122, 194, 572, 647, 793, 838, 1220
HincII GTYRAC 2 cut(s) 1144, 1158
HindII GTYRAC 2 cut(s) 1144, 1158
HindIII AAGCTT 3 cut(s) 479, 738, 845
HinfI GANTC 7 cut(s) 271, 294, 365, 600, 806, 1051, 1159
HpaII CCGG 3 cut(s) 902, 914, 982
HphI GGTGA 3 cut(s) 134, 278, 929
Hpy166II GTNNAC 3 cut(s) 157, 1144, 1158
Hpy188I TCNGA 9 cut(s) 7, 183, 220, 453, 547, 694, 1050, 1091, 1350
Hpy188III TCNNGA 6 cut(s) 53, 96, 131, 597, 1186, 1217
Hpy8I GTNNAC 3 cut(s) 157, 1144, 1158
HpyAV CCTTC 5 cut(s) 4, 770, 1001, 1370, 1428
HpyCH4III ACNGT 4 cut(s) 330, 439, 489, 1246
HpyCH4IV ACGT 2 cut(s) 817, 1110
HpyF10VI GCNNNNNNNGC 4 cut(s) 358, 853, 898, 1395
HpyF3I CTNAG 7 cut(s) 6, 318, 390, 864, 1056, 1131, 1263
HpySE526I ACGT 2 cut(s) 817, 1110
Hsp92II CATG 7 cut(s) 122, 194, 572, 647, 793, 838, 1220
Ksp22I TGATCA 2 cut(s) 220, 1030
Kzo9I GATC 5 cut(s) 172, 220, 547, 622, 1030
LguI GCTCTTC 1 cut(s) 567
LmnI GCTCC 1 cut(s) 349
Lsp1109I GCAGC 5 cut(s) 179, 381, 709, 1219, 1431
LweI GCATC 2 cut(s) 137, 1199
MaeI CTAG 6 cut(s) 24, 140, 152, 227, 536, 1466
MaeII ACGT 2 cut(s) 817, 1110
MaeIII GTNAC 7 cut(s) 122, 385, 443, 609, 823, 1106, 1404
MalI GATC 5 cut(s) 174, 222, 549, 624, 1032
MboI GATC 5 cut(s) 172, 220, 547, 622, 1030
MboII GAAGA 8 cut(s) 303, 335, 349, 584, 632, 678, 932, 1154
MfeI CAATTG 1 cut(s) 1095
MflI RGATCY 1 cut(s) 172
MhlI GDGCHC 1 cut(s) 1391
MlsI TGGCCA 1 cut(s) 1439
MluCI AATT 8 cut(s) 184, 299, 502, 838, 873, 1095, 1121, 1298
MluNI TGGCCA 1 cut(s) 1439
MlyI GAGTC 3 cut(s) 280, 609, 1153
MmeI TCCRAC 2 cut(s) 404, 783
MnlI CCTC 8 cut(s) 8, 11, 49, 156, 298, 502, 505, 1267
Mox20I TGGCCA 1 cut(s) 1439
Mph1103I ATGCAT 2 cut(s) 1214, 1400
MroXI GAANNNNTTC 1 cut(s) 1245
MscI TGGCCA 1 cut(s) 1439
MseI TTAA 3 cut(s) 63, 462, 1457
MslI CAYNNNNRTG 1 cut(s) 204
Msp20I TGGCCA 1 cut(s) 1439
MspI CCGG 3 cut(s) 902, 914, 982
MspR9I CCNGG 5 cut(s) 713, 915, 983, 1167, 1203
MunI CAATTG 1 cut(s) 1095
Mva1269I GAATGC 2 cut(s) 478, 1212
MvaI CCWGG 3 cut(s) 713, 1167, 1203
MwoI GCNNNNNNNGC 4 cut(s) 358, 853, 898, 1395
NciI CCSGG 2 cut(s) 915, 983
NdeII GATC 5 cut(s) 172, 220, 547, 622, 1030
NlaIII CATG 7 cut(s) 122, 194, 572, 647, 793, 838, 1220
NlaIV GGNNCC 1 cut(s) 912
NmuCI GTSAC 3 cut(s) 122, 443, 823
NsiI ATGCAT 2 cut(s) 1214, 1400
PagI TCATGA 1 cut(s) 1216
PciSI GCTCTTC 1 cut(s) 567
PctI GAATGC 2 cut(s) 478, 1212
PdmI GAANNNNTTC 1 cut(s) 1245
PfeI GAWTC 4 cut(s) 294, 365, 806, 1051
PflMI CCANNNNNTGG 1 cut(s) 111
PkrI GCNGC 6 cut(s) 169, 396, 724, 900, 1234, 1446
PleI GAGTC 3 cut(s) 279, 608, 1153
PpsI GAGTC 3 cut(s) 279, 608, 1153
PshBI ATTAAT 1 cut(s) 63
PsiI TTATAA 1 cut(s) 1119
Psp124BI GAGCTC 1 cut(s) 1391
Psp1406I AACGTT 1 cut(s) 1110
Psp6I CCWGG 3 cut(s) 711, 1165, 1201
PspGI CCWGG 3 cut(s) 711, 1165, 1201
PspN4I GGNNCC 1 cut(s) 912
PspPI GGNCC 3 cut(s) 98, 565, 934
PstI CTGCAG 3 cut(s) 399, 727, 1449
PsuI RGATCY 1 cut(s) 172
RsaI GTAC 3 cut(s) 491, 519, 953
RsaNI GTAC 3 cut(s) 490, 518, 952
RseI CAYNNNNRTG 1 cut(s) 204
SacI GAGCTC 1 cut(s) 1391
SalI GTCGAC 1 cut(s) 1156
SapI GCTCTTC 1 cut(s) 567
SaqAI TTAA 3 cut(s) 63, 462, 1457
SatI GCNGC 6 cut(s) 168, 395, 723, 899, 1233, 1445
Sau3AI GATC 5 cut(s) 172, 220, 547, 622, 1030
Sau96I GGNCC 3 cut(s) 98, 565, 934
ScaI AGTACT 1 cut(s) 953
SchI GAGTC 3 cut(s) 280, 609, 1153
ScrFI CCNGG 5 cut(s) 713, 915, 983, 1167, 1203
SduI GDGCHC 1 cut(s) 1391
SfaNI GCATC 2 cut(s) 137, 1199
SfcI CTRYAG 4 cut(s) 77, 395, 723, 1445
SinI GGWCC 2 cut(s) 98, 565
SmiMI CAYNNNNRTG 1 cut(s) 204
SmlI CTYRAG 1 cut(s) 408
SmoI CTYRAG 1 cut(s) 408
Sse9I AATT 8 cut(s) 184, 299, 502, 838, 873, 1095, 1121, 1298
SsiI CCGC 1 cut(s) 899
SspI AATATT 1 cut(s) 1225
SspMI CTAG 6 cut(s) 24, 140, 152, 227, 536, 1466
SstI GAGCTC 1 cut(s) 1391
StyD4I CCNGG 5 cut(s) 711, 913, 981, 1165, 1201
StyI CCWWGG 1 cut(s) 551
TaaI ACNGT 4 cut(s) 330, 439, 489, 1246
TaiI ACGT 2 cut(s) 820, 1113
TaqI TCGA 3 cut(s) 132, 1157, 1283
TasI AATT 8 cut(s) 184, 299, 502, 838, 873, 1095, 1121, 1298
TatI WGTACW 2 cut(s) 517, 951
TauI GCSGC 1 cut(s) 901
TfiI GAWTC 4 cut(s) 294, 365, 806, 1051
Tru1I TTAA 3 cut(s) 63, 462, 1457
Tru9I TTAA 3 cut(s) 63, 462, 1457
TscAI CASTG 4 cut(s) 335, 359, 832, 1428
TseFI GTSAC 3 cut(s) 122, 443, 823
TseI GCWGC 5 cut(s) 167, 394, 722, 1232, 1444
Tsp45I GTSAC 3 cut(s) 122, 443, 823
TspDTI ATGAA 9 cut(s) 188, 585, 632, 733, 851, 1020, 1166, 1205, 1238
TspGWI ACGGA 1 cut(s) 633
TspRI CASTG 4 cut(s) 335, 359, 832, 1428
Van91I CCANNNNNTGG 1 cut(s) 111
VpaK11BI GGWCC 2 cut(s) 98, 565
VspI ATTAAT 1 cut(s) 63
XapI RAATTY 1 cut(s) 184
XcmI CCANNNNNNNNNTGG 1 cut(s) 108
XmiI GTMKAC 1 cut(s) 1157
XmnI GAANNNNTTC 1 cut(s) 1245
XspI CTAG 6 cut(s) 24, 140, 152, 227, 536, 1466
ZrmI AGTACT 1 cut(s) 953
Zsp2I ATGCAT 2 cut(s) 1214, 1400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.