Rw7G009650

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0015539
Physical Location & Seq
Reverse (-)
9009026 .. 9010212
1187 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G009650.1

Sequence Viewer

Length: 963 bp
ATGGAGGAGATGGACGTTGATACTGAGGATCGAAACTTGACGGAGGTCCCTCCGACCAAGCGCCGGCGTCGACTGAGGGATGACGTGGCACCTCATTTATCCCTGCATTCATGTCCAAATGAATCACAGGATGATATGTTGGATATGAATTCAGGATTGATTGAGCGAGATGTCAGGAAGCAATCTGTAGAAGATCATGATTTAATTGAGGGAGGCTCCACATTAGGCAGGAAAGAAGCTGGTTTGCTTGGTAATGGGGCACGTGGGGTACAGGTGCCCACTTCAGAAGGTGGGCTACAGAGTGGGCTACAGAACCATGCTTCTAATTTGGCCCAATCCCACCCACTCAGACCGACTACAGCTCCAGATTCTAATCTCGAGATTGTATCTGCTGGGGGTTGTTTCTCAACCTCTCATTCTGATTCAAAATCTATGAATCAAAAGTCAAAACAGAAGAAAGGGATTTTTGAATTTGGGGAAGGCAAATCTGTAGGGGTGGAAGGGGATGATGTTAGATTTTACAAAAGGGGAGGTGGAAGAGGTCGGCGAAGTGATGGCAGAGAGGGTTTTGGGGGAGGAGGGGGGAGTACTAGGGACAAGGAGAAAGGGAAGGAAGTTGTCTCTGAAGGTGTGGAGAATGATGGGAAGCTAAATAGGACGAAAGATACGGCGGCATTGGGAGGTCGAGGTAGAGGTGGAAAGGGGCGCTATGGCTTTCCAGTCAGCGGCCAGGGGCGTGGGCACTCCTCCAACCGAGTTCCCCCACGAAAATCCAATCTTGCTGATGTCTCAGTGGGGAAGGTGGGTCATAGTAGCGGTGGTTTAGCCTTGTGCTGGAAGAGTCAGTTGGTCGTCAGGCCTTTGGGCTCTTCCCAAGGGCATATTGATGTTGAGATACAGCTGAATGGGGAAACTATCAGGGTCACTGGTTTTTATGGAAATCCAGAAACTCATCTCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

320

Amino Acids

34.16

Weight (kDa)

8.69

Isoelectric Point (pI)

49.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 88, 274
AccI GTMKAC 1 cut(s) 70
AciI CCGC 3 cut(s) 671, 726, 816
AclWI GGATC 1 cut(s) 36
AcoI YGGCCR 1 cut(s) 727
AcsI RAATTY 2 cut(s) 148, 470
AcuI CTGAAG 2 cut(s) 267, 645
AcvI CACGTG 1 cut(s) 263
AcyI GRCGYC 1 cut(s) 67
AfaI GTAC 2 cut(s) 270, 589
AfiI CCNNNNNNNGG 3 cut(s) 63, 725, 834
AgsI TTSAA 2 cut(s) 426, 470
AjiI CACGTC 1 cut(s) 85
AjnI CCWGG 1 cut(s) 729
AjuI GAANNNNNNNTTGG 2 cut(s) 830, 862
AluBI AGCT 4 cut(s) 239, 362, 649, 901
AluI AGCT 4 cut(s) 239, 362, 649, 901
Alw26I GTCTC 2 cut(s) 625, 793
AlwI GGATC 1 cut(s) 36
Ama87I CYCGRG 1 cut(s) 377
AoxI GGCC 3 cut(s) 330, 727, 857
ApoI RAATTY 2 cut(s) 148, 470
AspLEI GCGC 2 cut(s) 63, 708
AspS9I GGNCC 2 cut(s) 46, 331
AvaI CYCGRG 1 cut(s) 377
AvaII GGWCC 1 cut(s) 46
BaeGI GKGCMC 3 cut(s) 262, 279, 744
BanI GGYRCC 2 cut(s) 88, 274
BanII GRGCYC 1 cut(s) 869
BarI GAAGNNNNNNTAC 2 cut(s) 279, 311
BbrPI CACGTG 1 cut(s) 263
BccI CCATC 3 cut(s) 4, 548, 635
BceAI ACGGC 1 cut(s) 684
BciT130I CCWGG 1 cut(s) 731
BcoDI GTCTC 2 cut(s) 625, 793
BfaI CTAG 1 cut(s) 591
BfmI CTRYAG 5 cut(s) 186, 296, 308, 357, 489
BfoI RGCGCY 2 cut(s) 64, 709
BisI GCNGC 2 cut(s) 672, 727
BlsI GCNGC 2 cut(s) 673, 728
BmcAI AGTACT 1 cut(s) 589
Bme1390I CCNGG 1 cut(s) 731
Bme18I GGWCC 1 cut(s) 46
BmeT110I CYCGRG 1 cut(s) 377
BmgBI CACGTC 1 cut(s) 85
BmgT120I GGNCC 2 cut(s) 46, 331
BmiI GGNNCC 4 cut(s) 48, 90, 217, 276
BmrFI CCNGG 1 cut(s) 731
BoxI GACNNNNGTC 1 cut(s) 44
BplI GAGNNNNNCTC 2 cut(s) 200, 232
BpmI CTGGAG 1 cut(s) 348
BsaAI YACGTR 1 cut(s) 263
BsaBI GATNNNNATC 1 cut(s) 372
BsaHI GRCGYC 1 cut(s) 67
BsaJI CCNNGG 2 cut(s) 730, 874
BsaXI ACNNNNNCTCC 2 cut(s) 346, 376
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 725, 834
Bse118I RCCGGY 1 cut(s) 63
Bse1I ACTGG 2 cut(s) 719, 931
Bse8I GATNNNNATC 1 cut(s) 372
BseBI CCWGG 1 cut(s) 731
BseDI CCNNGG 2 cut(s) 730, 874
BseGI GGATG 3 cut(s) 85, 136, 511
BseJI GATNNNNATC 1 cut(s) 372
BseLI CCNNNNNNNGG 3 cut(s) 63, 725, 834
BseMII CTCAG 4 cut(s) 15, 65, 361, 804
BseNI ACTGG 2 cut(s) 719, 931
BseRI GAGGAG 3 cut(s) 20, 591, 736
BseSI GKGCMC 3 cut(s) 262, 279, 744
BseYI CCCAGC 1 cut(s) 392
BshFI GGCC 3 cut(s) 332, 729, 859
BshNI GGYRCC 2 cut(s) 88, 274
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 1 cut(s) 64
BslFI GGGAC 2 cut(s) 32, 608
BslI CCNNNNNNNGG 3 cut(s) 63, 725, 834
BsmAI GTCTC 2 cut(s) 625, 793
BsmFI GGGAC 2 cut(s) 32, 608
BsmI GAATGC 1 cut(s) 106
BsnI GGCC 3 cut(s) 332, 729, 859
BsoBI CYCGRG 1 cut(s) 377
Bsp1286I GDGCHC 4 cut(s) 262, 279, 744, 869
Bsp143I GATC 2 cut(s) 28, 193
BspACI CCGC 3 cut(s) 671, 726, 816
BspANI GGCC 3 cut(s) 332, 729, 859
BspCNI CTCAG 4 cut(s) 16, 66, 360, 803
BspHI TCATGA 1 cut(s) 196
BspLI GGNNCC 4 cut(s) 48, 90, 217, 276
BspPI GGATC 1 cut(s) 36
BspQI GCTCTTC 1 cut(s) 874
BspT107I GGYRCC 2 cut(s) 88, 274
BsrFI RCCGGY 1 cut(s) 63
BsrI ACTGG 2 cut(s) 719, 931
BssAI RCCGGY 1 cut(s) 63
BssECI CCNNGG 2 cut(s) 730, 874
BssMI GATC 2 cut(s) 28, 193
BssNI GRCGYC 1 cut(s) 67
BssT1I CCWWGG 1 cut(s) 874
Bst2UI CCWGG 1 cut(s) 731
Bst6I CTCTTC 3 cut(s) 532, 833, 874
BstACI GRCGYC 1 cut(s) 67
BstBAI YACGTR 1 cut(s) 263
BstC8I GCNNGC 1 cut(s) 65
BstDEI CTNAG 4 cut(s) 24, 74, 347, 790
BstF5I GGATG 3 cut(s) 85, 136, 511
BstH2I RGCGCY 2 cut(s) 64, 709
BstHHI GCGC 2 cut(s) 63, 708
BstKTI GATC 2 cut(s) 31, 196
BstMAI GTCTC 2 cut(s) 625, 793
BstMBI GATC 2 cut(s) 28, 193
BstNI CCWGG 1 cut(s) 731
BstPAI GACNNNNGTC 1 cut(s) 44
BstSCI CCNGG 1 cut(s) 729
BstSFI CTRYAG 5 cut(s) 186, 296, 308, 357, 489
BstSLI GKGCMC 3 cut(s) 262, 279, 744
BstXI CCANNNNNNTGG 1 cut(s) 737
BsuRI GGCC 3 cut(s) 332, 729, 859
BtrI CACGTC 1 cut(s) 85
BtsCI GGATG 3 cut(s) 85, 136, 511
BtsIMutI CAGTG 2 cut(s) 798, 924
Cac8I GCNNGC 1 cut(s) 65
CciI TCATGA 1 cut(s) 196
CfoI GCGC 2 cut(s) 63, 708
Cfr10I RCCGGY 1 cut(s) 63
Cfr13I GGNCC 2 cut(s) 46, 331
CseI GACGC 1 cut(s) 56
Csp6I GTAC 2 cut(s) 269, 588
CviAII CATG 3 cut(s) 111, 197, 317
CviQI GTAC 2 cut(s) 269, 588
DdeI CTNAG 4 cut(s) 24, 74, 347, 790
DpnI GATC 2 cut(s) 30, 195
DpnII GATC 2 cut(s) 28, 193
EaeI YGGCCR 1 cut(s) 727
Eam1104I CTCTTC 3 cut(s) 532, 833, 874
EarI CTCTTC 3 cut(s) 532, 833, 874
Eco130I CCWWGG 1 cut(s) 874
Eco147I AGGCCT 1 cut(s) 859
Eco24I GRGCYC 1 cut(s) 869
Eco47I GGWCC 1 cut(s) 46
Eco57I CTGAAG 2 cut(s) 267, 645
Eco72I CACGTG 1 cut(s) 263
Eco88I CYCGRG 1 cut(s) 377
EcoO109I RGGNCCY 1 cut(s) 46
EcoRI GAATTC 1 cut(s) 148
EcoRII CCWGG 1 cut(s) 729
EcoT14I CCWWGG 1 cut(s) 874
EcoT38I GRGCYC 1 cut(s) 869
ErhI CCWWGG 1 cut(s) 874
FaeI CATG 3 cut(s) 114, 200, 320
FaqI GGGAC 2 cut(s) 32, 608
FatI CATG 3 cut(s) 110, 196, 316
FblI GTMKAC 1 cut(s) 70
Fnu4HI GCNGC 2 cut(s) 672, 727
FokI GGATG 3 cut(s) 92, 143, 518
FriOI GRGCYC 1 cut(s) 869
Fsp4HI GCNGC 2 cut(s) 672, 727
FspBI CTAG 1 cut(s) 591
GlaI GCGC 2 cut(s) 62, 707
GluI GCNGC 2 cut(s) 672, 727
GsaI CCCAGC 1 cut(s) 396
GsuI CTGGAG 1 cut(s) 348
HaeII RGCGCY 2 cut(s) 64, 709
HaeIII GGCC 3 cut(s) 332, 729, 859
HapII CCGG 1 cut(s) 64
HgaI GACGC 1 cut(s) 56
HhaI GCGC 2 cut(s) 63, 708
Hin1I GRCGYC 1 cut(s) 67
Hin1II CATG 3 cut(s) 114, 200, 320
Hin6I GCGC 2 cut(s) 61, 706
HinP1I GCGC 2 cut(s) 61, 706
HincII GTYRAC 1 cut(s) 71
HindII GTYRAC 1 cut(s) 71
HinfI GANTC 5 cut(s) 122, 368, 422, 436, 841
HpaII CCGG 1 cut(s) 64
Hpy166II GTNNAC 1 cut(s) 71
Hpy188I TCNGA 5 cut(s) 54, 286, 350, 421, 625
Hpy188III TCNNGA 7 cut(s) 153, 175, 197, 365, 377, 379, 944
Hpy8I GTNNAC 1 cut(s) 71
Hpy99I CGWCG 1 cut(s) 72
HpyAV CCTTC 6 cut(s) 281, 473, 494, 604, 620, 793
HpyCH4IV ACGT 3 cut(s) 15, 84, 262
HpyCH4V TGCA 1 cut(s) 106
HpyF3I CTNAG 4 cut(s) 24, 74, 347, 790
HpySE526I ACGT 3 cut(s) 15, 84, 262
Hsp92I GRCGYC 1 cut(s) 67
Hsp92II CATG 3 cut(s) 114, 200, 320
HspAI GCGC 2 cut(s) 61, 706
KroI GCCGGC 1 cut(s) 63
KroNI GCCGGC 1 cut(s) 65
Kzo9I GATC 2 cut(s) 28, 193
LguI GCTCTTC 1 cut(s) 874
LmnI GCTCC 2 cut(s) 221, 367
MaeI CTAG 1 cut(s) 591
MaeII ACGT 3 cut(s) 15, 84, 262
MaeIII GTNAC 1 cut(s) 922
MalI GATC 2 cut(s) 30, 195
MboI GATC 2 cut(s) 28, 193
MboII GAAGA 5 cut(s) 203, 466, 549, 850, 861
MhlI GDGCHC 4 cut(s) 262, 279, 744, 869
MluCI AATT 4 cut(s) 148, 204, 325, 470
MlyI GAGTC 1 cut(s) 850
MmeI TCCRAC 3 cut(s) 77, 120, 774
MreI CGCCGGCG 1 cut(s) 63
MroNI GCCGGC 1 cut(s) 63
MseI TTAA 1 cut(s) 203
MslI CAYNNNNRTG 1 cut(s) 885
MspA1I CMGCKG 2 cut(s) 726, 901
MspI CCGG 1 cut(s) 64
MspR9I CCNGG 1 cut(s) 731
Mva1269I GAATGC 1 cut(s) 106
MvaI CCWGG 1 cut(s) 731
NaeI GCCGGC 1 cut(s) 65
NdeII GATC 2 cut(s) 28, 193
NgoMIV GCCGGC 1 cut(s) 63
NlaIII CATG 3 cut(s) 114, 200, 320
NlaIV GGNNCC 4 cut(s) 48, 90, 217, 276
NmuCI GTSAC 1 cut(s) 922
PaeR7I CTCGAG 1 cut(s) 377
PagI TCATGA 1 cut(s) 196
PceI AGGCCT 1 cut(s) 859
PciSI GCTCTTC 1 cut(s) 874
PctI GAATGC 1 cut(s) 106
PdiI GCCGGC 1 cut(s) 65
PfeI GAWTC 4 cut(s) 122, 368, 422, 436
PkrI GCNGC 2 cut(s) 673, 728
PleI GAGTC 1 cut(s) 849
PmaCI CACGTG 1 cut(s) 263
PmlI CACGTG 1 cut(s) 263
PpsI GAGTC 1 cut(s) 849
Ppu21I YACGTR 1 cut(s) 263
PpuMI RGGWCCY 1 cut(s) 46
PshAI GACNNNNGTC 1 cut(s) 44
Psp5II RGGWCCY 1 cut(s) 46
Psp6I CCWGG 1 cut(s) 729
PspCI CACGTG 1 cut(s) 263
PspFI CCCAGC 1 cut(s) 392
PspGI CCWGG 1 cut(s) 729
PspN4I GGNNCC 4 cut(s) 48, 90, 217, 276
PspPI GGNCC 2 cut(s) 46, 331
PspPPI RGGWCCY 1 cut(s) 46
PvuII CAGCTG 1 cut(s) 901
RsaI GTAC 2 cut(s) 270, 589
RsaNI GTAC 2 cut(s) 269, 588
RseI CAYNNNNRTG 1 cut(s) 885
SalI GTCGAC 1 cut(s) 69
SapI GCTCTTC 1 cut(s) 874
SaqAI TTAA 1 cut(s) 203
SatI GCNGC 2 cut(s) 672, 727
Sau3AI GATC 2 cut(s) 28, 193
Sau96I GGNCC 2 cut(s) 46, 331
ScaI AGTACT 1 cut(s) 589
SchI GAGTC 1 cut(s) 850
ScrFI CCNGG 1 cut(s) 731
SduI GDGCHC 4 cut(s) 262, 279, 744, 869
SfcI CTRYAG 5 cut(s) 186, 296, 308, 357, 489
Sfr274I CTCGAG 1 cut(s) 377
SgrAI CRCCGGYG 1 cut(s) 63
SinI GGWCC 1 cut(s) 46
SlaI CTCGAG 1 cut(s) 377
SmiMI CAYNNNNRTG 1 cut(s) 885
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 4 cut(s) 148, 204, 325, 470
SseBI AGGCCT 1 cut(s) 859
SsiI CCGC 3 cut(s) 671, 726, 816
SspMI CTAG 1 cut(s) 591
StuI AGGCCT 1 cut(s) 859
StyD4I CCNGG 1 cut(s) 729
StyI CCWWGG 1 cut(s) 874
TaiI ACGT 3 cut(s) 18, 87, 265
TaqI TCGA 4 cut(s) 31, 70, 378, 685
TaqII GACCGA 1 cut(s) 367
TasI AATT 4 cut(s) 148, 204, 325, 470
TatI WGTACW 1 cut(s) 587
TauI GCSGC 2 cut(s) 674, 729
TfiI GAWTC 4 cut(s) 122, 368, 422, 436
Tru1I TTAA 1 cut(s) 203
Tru9I TTAA 1 cut(s) 203
TscAI CASTG 2 cut(s) 798, 931
TseFI GTSAC 1 cut(s) 922
Tsp45I GTSAC 1 cut(s) 922
TspDTI ATGAA 4 cut(s) 99, 135, 161, 449
TspGWI ACGGA 1 cut(s) 56
TspRI CASTG 2 cut(s) 798, 931
VpaK11BI GGWCC 1 cut(s) 46
XapI RAATTY 2 cut(s) 148, 470
XhoI CTCGAG 1 cut(s) 377
XmiI GTMKAC 1 cut(s) 70
XspI CTAG 1 cut(s) 591
ZrmI AGTACT 1 cut(s) 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.