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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 1 cut(s) | 194 |
| AjnI | CCWGG | 1 cut(s) | 58 |
| AluBI | AGCT | 1 cut(s) | 125 |
| AluI | AGCT | 1 cut(s) | 125 |
| ApeKI | GCWGC | 1 cut(s) | 151 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 171 |
| AspS9I | GGNCC | 1 cut(s) | 55 |
| AvaII | GGWCC | 1 cut(s) | 55 |
| BbsI | GAAGAC | 1 cut(s) | 79 |
| BbvI | GCAGC | 1 cut(s) | 163 |
| BciT130I | CCWGG | 1 cut(s) | 60 |
| BfmI | CTRYAG | 1 cut(s) | 149 |
| BisI | GCNGC | 1 cut(s) | 152 |
| BlsI | GCNGC | 1 cut(s) | 153 |
| Bme1390I | CCNGG | 1 cut(s) | 60 |
| Bme18I | GGWCC | 1 cut(s) | 55 |
| BmgT120I | GGNCC | 1 cut(s) | 55 |
| BmiI | GGNNCC | 1 cut(s) | 57 |
| BmrFI | CCNGG | 1 cut(s) | 60 |
| BpiI | GAAGAC | 1 cut(s) | 79 |
| BsaJI | CCNNGG | 1 cut(s) | 58 |
| BseBI | CCWGG | 1 cut(s) | 60 |
| BseDI | CCNNGG | 1 cut(s) | 58 |
| BseXI | GCAGC | 1 cut(s) | 163 |
| BslFI | GGGAC | 1 cut(s) | 41 |
| BsmFI | GGGAC | 1 cut(s) | 41 |
| BspLI | GGNNCC | 1 cut(s) | 57 |
| BspMAI | CTGCAG | 1 cut(s) | 153 |
| BssECI | CCNNGG | 1 cut(s) | 58 |
| Bst2UI | CCWGG | 1 cut(s) | 60 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 160 |
| BstDEI | CTNAG | 2 cut(s) | 68, 77 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 131, 160 |
| BstNI | CCWGG | 1 cut(s) | 60 |
| BstSCI | CCNGG | 1 cut(s) | 58 |
| BstSFI | CTRYAG | 1 cut(s) | 149 |
| BstV1I | GCAGC | 1 cut(s) | 163 |
| BstV2I | GAAGAC | 1 cut(s) | 79 |
| Cfr13I | GGNCC | 1 cut(s) | 55 |
| CviAII | CATG | 1 cut(s) | 203 |
| CviJI | RGCY | 1 cut(s) | 125 |
| CviKI_1 | RGCY | 1 cut(s) | 125 |
| DdeI | CTNAG | 2 cut(s) | 68, 77 |
| Eco47I | GGWCC | 1 cut(s) | 55 |
| EcoRII | CCWGG | 1 cut(s) | 58 |
| EcoT22I | ATGCAT | 1 cut(s) | 6 |
| FaeI | CATG | 1 cut(s) | 206 |
| FaiI | YATR | 3 cut(s) | 156, 204, 220 |
| FaqI | GGGAC | 1 cut(s) | 41 |
| FatI | CATG | 1 cut(s) | 202 |
| Fnu4HI | GCNGC | 1 cut(s) | 152 |
| Fsp4HI | GCNGC | 1 cut(s) | 152 |
| GluI | GCNGC | 1 cut(s) | 152 |
| Hin1II | CATG | 1 cut(s) | 206 |
| Hpy188III | TCNNGA | 1 cut(s) | 176 |
| HpyAV | CCTTC | 2 cut(s) | 82, 89 |
| HpyCH4V | TGCA | 3 cut(s) | 4, 151, 163 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 131, 160 |
| HpyF3I | CTNAG | 2 cut(s) | 68, 77 |
| Hsp92II | CATG | 1 cut(s) | 206 |
| LpnPI | CCDG | 3 cut(s) | 45, 72, 161 |
| Lsp1109I | GCAGC | 1 cut(s) | 163 |
| MboII | GAAGA | 2 cut(s) | 84, 122 |
| MfeI | CAATTG | 1 cut(s) | 116 |
| MluCI | AATT | 3 cut(s) | 116, 158, 214 |
| MnlI | CCTC | 2 cut(s) | 35, 85 |
| Mph1103I | ATGCAT | 1 cut(s) | 6 |
| MroXI | GAANNNNTTC | 1 cut(s) | 171 |
| MspR9I | CCNGG | 1 cut(s) | 60 |
| MunI | CAATTG | 1 cut(s) | 116 |
| MvaI | CCWGG | 1 cut(s) | 60 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 131, 160 |
| NlaIII | CATG | 1 cut(s) | 206 |
| NlaIV | GGNNCC | 1 cut(s) | 57 |
| NsiI | ATGCAT | 1 cut(s) | 6 |
| PdmI | GAANNNNTTC | 1 cut(s) | 171 |
| PkrI | GCNGC | 1 cut(s) | 153 |
| Psp6I | CCWGG | 1 cut(s) | 58 |
| PspGI | CCWGG | 1 cut(s) | 58 |
| PspN4I | GGNNCC | 1 cut(s) | 57 |
| PspPI | GGNCC | 1 cut(s) | 55 |
| PstI | CTGCAG | 1 cut(s) | 153 |
| SatI | GCNGC | 1 cut(s) | 152 |
| Sau96I | GGNCC | 1 cut(s) | 55 |
| ScrFI | CCNGG | 1 cut(s) | 60 |
| SetI | ASST | 3 cut(s) | 100, 127, 189 |
| SfcI | CTRYAG | 1 cut(s) | 149 |
| SgeI | CNNG | 9 cut(s) | 25, 45, 51, 62, 71, 72, 176, 188, 215 |
| SinI | GGWCC | 1 cut(s) | 55 |
| Sse9I | AATT | 3 cut(s) | 116, 158, 214 |
| StyD4I | CCNGG | 1 cut(s) | 58 |
| TaqI | TCGA | 1 cut(s) | 12 |
| TasI | AATT | 3 cut(s) | 116, 158, 214 |
| TseI | GCWGC | 1 cut(s) | 151 |
| TspDTI | ATGAA | 2 cut(s) | 42, 207 |
| VpaK11BI | GGWCC | 1 cut(s) | 55 |
| XmnI | GAANNNNTTC | 1 cut(s) | 171 |
| Zsp2I | ATGCAT | 1 cut(s) | 6 |