Prupe.4G254100_v2.0.a1

NADH kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000832
Physical Location & Seq
Reverse (-)
17445106 .. 17456983
11878 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G254100.26

Sequence Viewer

Length: 1701 bp
ATGGCTCCCAGCAAGGTCAATTCTGCTGGTGATGCAAGTGTATCATGCTCACAGCCAGAGAATGGTTTTCTCAATTCACTTTCTCTCTTAAGTTCAGAGAAAGCAGTGCAGGAACTTCTGCAACAATCTCCTGTACAGAGCACAGATGACCACCTCATAGAGTTCTCAGACGCTATGAGAACTGTTGCAAAGGCCCTGAGACGGGCTGCTGAAGGAAAGGCTTCTGCGCAAGCTGAGGCAGCTGAATGGAAGCGTAAATATGAACTGGAGAGGGCCCGCAATCTCCTTTTGGAACATAAAGGCTACAACTTTTATGAAAAGCTCGAAAGTTGGTGGACTCAGAGTTCTCTATGTAATGCAATGGGAATGATAAGATTTATGCTTCCATGCCTTTTCTGCTTGGAAAATTGCAAGGAACGGGACAGGAAAATTTCTGAAGAGCAATCACATAGAGAGAATAACGGTGATGATGAGAGGACAGAGAACTTGGCCAGCCGATCAAATGAGCAATCTGAACAGTGTTGTGGGAGTAATGGGATATGCTCCCATGAAGTTCTTCGGGATGGTGACCGTGATTCTGCTTCCAAGGTGGTCCCCAATAAATGTGCCAGAAAGGCGTCATTTAAACTTTCATGGTGTTGCAAAGGTGACCAAAGTGACCAGCACAAGCATGACATTGTCTCTTTTGAAAGAGGAAACATTACAACTGCAGAGCGCAGCAGTAAACAAATTTCTTTGAAGTGGGAATCTCAGCCACAAACTGTGATTATATTGACCAAACCAAATTCAACTTCTGTTCGAATTCTGTGTGCAGAGATGGTCAGATGGTTGAGAGAGCAGAAAAAGTTAGACATTTATGTGGAACCGCGCGTGAGAGCTGAACTTCTAACAGAGTCATCTTACTACAACTTCGTCCATACATGGAAAGGTGAGACCGAAATAATGCTCCTGCACACAAAAGTTGACCTTGTGGTAACTCTTGGTGGAGATGGAACTGTCCTCTGGGCAGCATCTATGTTCAAAGGACCAGTTCCTCCTGTTGTGCCGTTTTCTTTAGGGTCTCTGGGATTTATGACCCCTTTTCACAGTGAACGTTACCAAGAATATCTTGATTCAATTTTAAAAGGTCCCATCAGTATCACATTGCGACACCGCTTGCAGTGCCATGTTATTAGAGAAGCAGCCAAAAATGAGTATGAAACTGAAGGACCGATACTTGTATTAAATGAGGTTACAATTGACCGTGGGATATCATCATACCTTACAAATTTGGAATGCTATTGTGACAATTCTTTTGTCACATGTGTCCAAGGAGATGGGCTAATATTATCAACAACATCTGGCAGTACTGCCTATTCATTAGCGGCCGGAGGATCAATGGTTCATCCCCAGGTTCCTGGAATTCTGTTTACACCTATTTGTCCACATTCCCTATCATTTCGGCCTCTGATATTGCCTGAGCATGTGACGCTGCGAGTACAAGTACCTTTCAACAGCAGGAGCCATGCATGGGCGTCGTTTGATGGCAAGGATAGGAAACGGTTGGCAGCTGGTGATGCACTTGTGTGCAGCATGGCACCTTGGCCTGTCCCAACTGCCTGTCAAGTTGATTCTACTAGTGACTTCCTGAACAGCATTCATGACGGCCTCCACTGGAATCTTCGAAAGACTCAGTCTTTTGATGGCCCTCGGGATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

567

Amino Acids

63.32

Weight (kDa)

6.3

Isoelectric Point (pI)

46.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G21070 AT3G21070 AT3G21070 AT3G21070
fragaria_vesca FvH4_3g28510
malus_domestica MD03G1172500.v1.1 MD11G1190400.v1.1
prunus_persica Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1
pyrus_communis pycom03g12970 pycom11g16670
rosa_chinensis RchiOBHm_Chr5g0052801
rosa_laevigata RLG00000034795
rosa_multiflora Rmu_sc0004335.1_g000006 Rmu_sc0032421.1_g000001 Rmu_ssc0000311.1_g000025
rosa_roxburghii Rroxscaffold_1G00028620 Rroxscaffold_4G00318540
rosa_rugosa Rorug05G0269700 Rorug05G0269800
rosa_samantha Rh5AG341600 Rh5BG352300 Rh5CG379000 Rh5DG368700
rosa_wichuraiana Rw5G032210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 228
AccB1I GGYRCC 1 cut(s) 1576
AccB7I CCANNNNNTGG 1 cut(s) 62
AccII CGCG 2 cut(s) 868, 870
AciI CCGC 4 cut(s) 277, 866, 1153, 1364
AclI AACGTT 1 cut(s) 1093
AclWI GGATC 1 cut(s) 1381
AcoI YGGCCR 2 cut(s) 489, 1365
AcsI RAATTY 6 cut(s) 429, 729, 784, 801, 1267, 1401
AcuI CTGAAG 3 cut(s) 231, 456, 1224
AcyI GRCGYC 2 cut(s) 617, 1514
AfaI GTAC 4 cut(s) 135, 1348, 1479, 1485
AfiI CCNNNNNNNGG 4 cut(s) 62, 201, 202, 1510
AflII CTTAAG 1 cut(s) 88
AflIII ACRYGT 1 cut(s) 1301
AgsI TTSAA 6 cut(s) 689, 739, 789, 1021, 1116, 1492
AhlI ACTAGT 1 cut(s) 1616
AjnI CCWGG 2 cut(s) 1389, 1396
AjuI GAANNNNNNNTTGG 2 cut(s) 775, 807
AleI CACNNNNGTG 1 cut(s) 1564
AloI GAACNNNNNNTCC 4 cut(s) 328, 360, 1365, 1397
AluBI AGCT 5 cut(s) 233, 242, 322, 878, 1550
AluI AGCT 5 cut(s) 233, 242, 322, 878, 1550
Alw21I GWGCWC 1 cut(s) 143
Alw26I GTCTC 4 cut(s) 193, 685, 926, 1065
AlwI GGATC 1 cut(s) 1381
Ama87I CYCGRG 1 cut(s) 1689
AoxI GGCC 8 cut(s) 192, 273, 489, 1365, 1442, 1583, 1645, 1684
ApaI GGGCCC 1 cut(s) 277
ApeKI GCWGC 8 cut(s) 206, 239, 717, 1007, 1181, 1471, 1547, 1569
ApoI RAATTY 6 cut(s) 429, 729, 784, 801, 1267, 1401
ArsI GACNNNNNNTTYG 2 cut(s) 1277, 1309
Asp700I GAANNNNTTC 2 cut(s) 220, 555
AspLEI GCGC 3 cut(s) 229, 717, 870
AspS9I GGNCC 8 cut(s) 193, 273, 274, 592, 1025, 1127, 1208, 1685
AsuHPI GGTGA 6 cut(s) 41, 476, 578, 659, 941, 1565
AsuII TTCGAA 2 cut(s) 799, 1663
AvaI CYCGRG 1 cut(s) 1689
AvaII GGWCC 4 cut(s) 592, 1025, 1127, 1208
BaeGI GKGCMC 1 cut(s) 277
BalI TGGCCA 1 cut(s) 491
BanI GGYRCC 1 cut(s) 1576
BanII GRGCYC 1 cut(s) 277
BarI GAAGNNNNNNTAC 2 cut(s) 1197, 1229
Bbv12I GWGCWC 1 cut(s) 143
BbvCI CCTCAGC 1 cut(s) 234
BbvI GCAGC 8 cut(s) 193, 251, 729, 1019, 1193, 1458, 1559, 1581
BccI CCATC 8 cut(s) 557, 811, 819, 983, 1139, 1310, 1517, 1676
BceAI ACGGC 2 cut(s) 1030, 1660
BcgI CGANNNNNNTGC 2 cut(s) 1497, 1531
BciT130I CCWGG 2 cut(s) 1391, 1398
BcoDI GTCTC 4 cut(s) 193, 685, 926, 1065
BcuI ACTAGT 1 cut(s) 1616
BfaI CTAG 1 cut(s) 1617
BfmI CTRYAG 1 cut(s) 708
BfrI CTTAAG 1 cut(s) 88
BglI GCCNNNNNGGC 1 cut(s) 614
BisI GCNGC 9 cut(s) 207, 240, 718, 1008, 1182, 1365, 1472, 1548, 1570
BlsI GCNGC 9 cut(s) 208, 241, 719, 1009, 1183, 1366, 1473, 1549, 1571
BmcAI AGTACT 1 cut(s) 1348
Bme1390I CCNGG 2 cut(s) 1391, 1398
Bme18I GGWCC 4 cut(s) 592, 1025, 1127, 1208
BmeT110I CYCGRG 1 cut(s) 1689
BmgT120I GGNCC 8 cut(s) 193, 273, 274, 592, 1025, 1127, 1208, 1685
BmiI GGNNCC 8 cut(s) 6, 275, 594, 864, 1129, 1395, 1502, 1578
BmrFI CCNGG 2 cut(s) 1391, 1398
BmsI GCATC 4 cut(s) 22, 1019, 1546, 1684
BpmI CTGGAG 1 cut(s) 287
Bpu10I CCTNAGC 2 cut(s) 234, 1458
Bpu14I TTCGAA 2 cut(s) 799, 1663
BsaHI GRCGYC 2 cut(s) 617, 1514
BsaI GGTCTC 2 cut(s) 926, 1065
BsaJI CCNNGG 6 cut(s) 585, 1243, 1309, 1389, 1580, 1688
Bsc4I CCNNNNNNNGG 4 cut(s) 62, 201, 202, 1510
Bse1I ACTGG 3 cut(s) 270, 1028, 1658
Bse3DI GCAATG 2 cut(s) 366, 1142
BseBI CCWGG 2 cut(s) 1391, 1398
BseDI CCNNGG 6 cut(s) 585, 1243, 1309, 1389, 1580, 1688
BseGI GGATG 3 cut(s) 568, 1384, 1699
BseLI CCNNNNNNNGG 4 cut(s) 62, 201, 202, 1510
BseMI GCAATG 2 cut(s) 366, 1142
BseMII CTCAG 7 cut(s) 180, 188, 225, 353, 764, 1449, 1685
BseNI ACTGG 3 cut(s) 270, 1028, 1658
BseSI GKGCMC 1 cut(s) 277
BseX3I CGGCCG 1 cut(s) 1365
BseXI GCAGC 8 cut(s) 193, 251, 729, 1019, 1193, 1458, 1559, 1581
BseYI CCCAGC 1 cut(s) 8
BsgI GTGCAG 4 cut(s) 128, 831, 935, 1588
Bsh1236I CGCG 2 cut(s) 868, 870
Bsh1285I CGRYCG 1 cut(s) 1368
BshFI GGCC 8 cut(s) 194, 275, 491, 1367, 1444, 1585, 1647, 1686
BshNI GGYRCC 1 cut(s) 1576
BsiEI CGRYCG 1 cut(s) 1368
BsiHKAI GWGCWC 1 cut(s) 143
BsiHKCI CYCGRG 1 cut(s) 1689
BsiSI CCGG 1 cut(s) 1368
BslFI GGGAC 4 cut(s) 434, 578, 1113, 1574
BslI CCNNNNNNNGG 4 cut(s) 62, 201, 202, 1510
BsmAI GTCTC 4 cut(s) 193, 685, 926, 1065
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 4 cut(s) 434, 578, 1113, 1574
BsmI GAATGC 2 cut(s) 1280, 1635
BsnI GGCC 8 cut(s) 194, 275, 491, 1367, 1444, 1585, 1647, 1686
Bso31I GGTCTC 2 cut(s) 926, 1065
BsoBI CYCGRG 1 cut(s) 1689
Bsp119I TTCGAA 2 cut(s) 799, 1663
Bsp120I GGGCCC 1 cut(s) 273
Bsp1286I GDGCHC 2 cut(s) 143, 277
Bsp1407I TGTACA 1 cut(s) 133
Bsp143I GATC 2 cut(s) 497, 1373
BspACI CCGC 4 cut(s) 277, 866, 1153, 1364
BspANI GGCC 8 cut(s) 194, 275, 491, 1367, 1444, 1585, 1647, 1686
BspCNI CTCAG 7 cut(s) 179, 189, 226, 352, 763, 1450, 1684
BspFNI CGCG 2 cut(s) 868, 870
BspHI TCATGA 1 cut(s) 1639
BspLI GGNNCC 8 cut(s) 6, 275, 594, 864, 1129, 1395, 1502, 1578
BspMAI CTGCAG 1 cut(s) 712
BspPI GGATC 1 cut(s) 1381
BspQI GCTCTTC 1 cut(s) 432
BspT104I TTCGAA 2 cut(s) 799, 1663
BspT107I GGYRCC 1 cut(s) 1576
BspTI CTTAAG 1 cut(s) 88
BspTNI GGTCTC 2 cut(s) 926, 1065
BsrDI GCAATG 2 cut(s) 366, 1142
BsrGI TGTACA 1 cut(s) 133
BsrI ACTGG 3 cut(s) 270, 1028, 1658
BssECI CCNNGG 6 cut(s) 585, 1243, 1309, 1389, 1580, 1688
BssMI GATC 2 cut(s) 497, 1373
BssNI GRCGYC 2 cut(s) 617, 1514
BssT1I CCWWGG 3 cut(s) 585, 1309, 1580
Bst2UI CCWGG 2 cut(s) 1391, 1398
Bst4CI ACNGT 9 cut(s) 184, 464, 519, 572, 763, 997, 1088, 1244, 1542
Bst6I CTCTTC 1 cut(s) 432
BstACI GRCGYC 2 cut(s) 617, 1514
BstAFI CTTAAG 1 cut(s) 88
BstAUI TGTACA 1 cut(s) 133
BstBI TTCGAA 2 cut(s) 799, 1663
BstC8I GCNNGC 4 cut(s) 231, 277, 493, 1157
BstDEI CTNAG 7 cut(s) 166, 197, 234, 339, 750, 1458, 1671
BstDSI CCRYGG 1 cut(s) 1243
BstEII GGTNACC 2 cut(s) 566, 647
BstF5I GGATG 3 cut(s) 568, 1384, 1699
BstFNI CGCG 2 cut(s) 868, 870
BstHHI GCGC 3 cut(s) 229, 717, 870
BstKTI GATC 2 cut(s) 500, 1376
BstMAI GTCTC 4 cut(s) 193, 685, 926, 1065
BstMBI GATC 2 cut(s) 497, 1373
BstMCI CGRYCG 1 cut(s) 1368
BstMWI GCNNNNNNNGC 7 cut(s) 32, 239, 396, 614, 1161, 1468, 1556
BstNI CCWGG 2 cut(s) 1391, 1398
BstNSI RCATGY 2 cut(s) 1305, 1466
BstPI GGTNACC 2 cut(s) 566, 647
BstSCI CCNGG 2 cut(s) 1389, 1396
BstSFI CTRYAG 1 cut(s) 708
BstSLI GKGCMC 1 cut(s) 277
BstUI CGCG 2 cut(s) 868, 870
BstV1I GCAGC 8 cut(s) 193, 251, 729, 1019, 1193, 1458, 1559, 1581
BstXI CCANNNNNNTGG 2 cut(s) 1316, 1397
BstZI CGGCCG 1 cut(s) 1365
BsuRI GGCC 8 cut(s) 194, 275, 491, 1367, 1444, 1585, 1647, 1686
BtgI CCRYGG 1 cut(s) 1243
BtsCI GGATG 3 cut(s) 568, 1384, 1699
BtsI GCAGTG 2 cut(s) 111, 1166
BtsIMutI CAGTG 5 cut(s) 111, 524, 1093, 1166, 1651
Cac8I GCNNGC 4 cut(s) 231, 277, 493, 1157
CciI TCATGA 1 cut(s) 1639
CfoI GCGC 3 cut(s) 229, 717, 870
Cfr13I GGNCC 8 cut(s) 193, 273, 274, 592, 1025, 1127, 1208, 1685
CseI GACGC 4 cut(s) 179, 606, 1477, 1503
Csp6I GTAC 4 cut(s) 134, 1347, 1478, 1484
CviQI GTAC 4 cut(s) 134, 1347, 1478, 1484
DdeI CTNAG 7 cut(s) 166, 197, 234, 339, 750, 1458, 1671
DpnI GATC 2 cut(s) 499, 1375
DpnII GATC 2 cut(s) 497, 1373
DraI TTTAAA 2 cut(s) 625, 1122
EaeI YGGCCR 2 cut(s) 489, 1365
EagI CGGCCG 1 cut(s) 1365
Eam1104I CTCTTC 1 cut(s) 432
EarI CTCTTC 1 cut(s) 432
EclXI CGGCCG 1 cut(s) 1365
Eco130I CCWWGG 3 cut(s) 585, 1309, 1580
Eco24I GRGCYC 1 cut(s) 277
Eco31I GGTCTC 2 cut(s) 926, 1065
Eco32I GATATC 1 cut(s) 1251
Eco47I GGWCC 4 cut(s) 592, 1025, 1127, 1208
Eco52I CGGCCG 1 cut(s) 1365
Eco57I CTGAAG 3 cut(s) 231, 456, 1224
Eco88I CYCGRG 1 cut(s) 1689
Eco91I GGTNACC 2 cut(s) 566, 647
EcoO109I RGGNCCY 3 cut(s) 193, 273, 1127
EcoO65I GGTNACC 2 cut(s) 566, 647
EcoRI GAATTC 2 cut(s) 801, 1401
EcoRII CCWGG 2 cut(s) 1389, 1396
EcoRV GATATC 1 cut(s) 1251
EcoT14I CCWWGG 3 cut(s) 585, 1309, 1580
EcoT22I ATGCAT 2 cut(s) 1510, 1699
EcoT38I GRGCYC 1 cut(s) 277
ErhI CCWWGG 3 cut(s) 585, 1309, 1580
Esp3I CGTCTC 1 cut(s) 193
FalI AAGNNNNNCTT 4 cut(s) 951, 983, 1092, 1124
FaqI GGGAC 4 cut(s) 434, 578, 1113, 1574
FauI CCCGC 1 cut(s) 284
Fnu4HI GCNGC 9 cut(s) 207, 240, 718, 1008, 1182, 1365, 1472, 1548, 1570
FokI GGATG 2 cut(s) 575, 1371
FriOI GRGCYC 1 cut(s) 277
Fsp4HI GCNGC 9 cut(s) 207, 240, 718, 1008, 1182, 1365, 1472, 1548, 1570
FspBI CTAG 1 cut(s) 1617
FspI TGCGCA 1 cut(s) 228
GlaI GCGC 3 cut(s) 228, 716, 869
GluI GCNGC 9 cut(s) 207, 240, 718, 1008, 1182, 1365, 1472, 1548, 1570
GsaI CCCAGC 1 cut(s) 12
GsuI CTGGAG 1 cut(s) 287
HaeIII GGCC 8 cut(s) 194, 275, 491, 1367, 1444, 1585, 1647, 1686
HapII CCGG 1 cut(s) 1368
HgaI GACGC 4 cut(s) 179, 606, 1477, 1503
HhaI GCGC 3 cut(s) 229, 717, 870
Hin1I GRCGYC 2 cut(s) 617, 1514
Hin6I GCGC 3 cut(s) 227, 715, 868
HinP1I GCGC 3 cut(s) 227, 715, 868
HincII GTYRAC 1 cut(s) 964
HindII GTYRAC 1 cut(s) 964
HinfI GANTC 8 cut(s) 337, 575, 746, 893, 1112, 1610, 1657, 1669
HpaII CCGG 1 cut(s) 1368
HphI GGTGA 6 cut(s) 41, 476, 578, 659, 941, 1565
Hpy166II GTNNAC 6 cut(s) 336, 725, 964, 1091, 1410, 1424
Hpy188I TCNGA 7 cut(s) 97, 169, 342, 436, 514, 824, 1449
Hpy188III TCNNGA 5 cut(s) 560, 1109, 1627, 1640, 1691
Hpy8I GTNNAC 6 cut(s) 336, 725, 964, 1091, 1410, 1424
Hpy99I CGWCG 1 cut(s) 1519
HpyAV CCTTC 2 cut(s) 206, 1199
HpyCH4III ACNGT 9 cut(s) 184, 464, 519, 572, 763, 997, 1088, 1244, 1542
HpyCH4IV ACGT 1 cut(s) 1093
HpyF10VI GCNNNNNNNGC 7 cut(s) 32, 239, 396, 614, 1161, 1468, 1556
HpyF3I CTNAG 7 cut(s) 166, 197, 234, 339, 750, 1458, 1671
HpySE526I ACGT 1 cut(s) 1093
Hsp92I GRCGYC 2 cut(s) 617, 1514
HspAI GCGC 3 cut(s) 227, 715, 868
Kzo9I GATC 2 cut(s) 497, 1373
LguI GCTCTTC 1 cut(s) 432
LmnI GCTCC 4 cut(s) 10, 548, 951, 1500
Lsp1109I GCAGC 8 cut(s) 193, 251, 729, 1019, 1193, 1458, 1559, 1581
LweI GCATC 4 cut(s) 22, 1019, 1546, 1684
MaeI CTAG 1 cut(s) 1617
MaeII ACGT 1 cut(s) 1093
MalI GATC 2 cut(s) 499, 1375
MboI GATC 2 cut(s) 497, 1373
MboII GAAGA 3 cut(s) 449, 548, 1652
MfeI CAATTG 1 cut(s) 1236
MhlI GDGCHC 2 cut(s) 143, 277
MlsI TGGCCA 1 cut(s) 491
MluNI TGGCCA 1 cut(s) 491
MlyI GAGTC 3 cut(s) 331, 902, 1663
Mox20I TGGCCA 1 cut(s) 491
Mph1103I ATGCAT 2 cut(s) 1510, 1699
MroXI GAANNNNTTC 2 cut(s) 220, 555
MscI TGGCCA 1 cut(s) 491
MseI TTAA 4 cut(s) 89, 624, 1121, 1223
MslI CAYNNNNRTG 3 cut(s) 669, 857, 1564
Msp20I TGGCCA 1 cut(s) 491
MspA1I CMGCKG 2 cut(s) 242, 1550
MspCI CTTAAG 1 cut(s) 88
MspI CCGG 1 cut(s) 1368
MspR9I CCNGG 2 cut(s) 1391, 1398
MunI CAATTG 1 cut(s) 1236
Mva1269I GAATGC 2 cut(s) 1280, 1635
MvaI CCWGG 2 cut(s) 1391, 1398
MvnI CGCG 2 cut(s) 868, 870
MwoI GCNNNNNNNGC 7 cut(s) 32, 239, 396, 614, 1161, 1468, 1556
NdeII GATC 2 cut(s) 497, 1373
NlaIV GGNNCC 8 cut(s) 6, 275, 594, 864, 1129, 1395, 1502, 1578
NmuCI GTSAC 7 cut(s) 566, 647, 656, 1283, 1297, 1465, 1619
NsbI TGCGCA 1 cut(s) 228
NsiI ATGCAT 2 cut(s) 1510, 1699
NspI RCATGY 2 cut(s) 1305, 1466
NspV TTCGAA 2 cut(s) 799, 1663
OliI CACNNNNGTG 1 cut(s) 1564
PagI TCATGA 1 cut(s) 1639
PciI ACATGT 1 cut(s) 1301
PciSI GCTCTTC 1 cut(s) 432
PctI GAATGC 2 cut(s) 1280, 1635
PdmI GAANNNNTTC 2 cut(s) 220, 555
PfeI GAWTC 5 cut(s) 575, 746, 1112, 1610, 1657
PflFI GACNNNGTC 2 cut(s) 677, 1672
PflMI CCANNNNNTGG 1 cut(s) 62
PfoI TCCNGGA 1 cut(s) 1396
PkrI GCNGC 9 cut(s) 208, 241, 719, 1009, 1183, 1366, 1473, 1549, 1571
PleI GAGTC 3 cut(s) 331, 901, 1663
PpsI GAGTC 3 cut(s) 331, 901, 1663
PpuMI RGGWCCY 1 cut(s) 1127
PscI ACATGT 1 cut(s) 1301
Psp1406I AACGTT 1 cut(s) 1093
Psp5II RGGWCCY 1 cut(s) 1127
Psp6I CCWGG 2 cut(s) 1389, 1396
PspEI GGTNACC 2 cut(s) 566, 647
PspFI CCCAGC 1 cut(s) 8
PspGI CCWGG 2 cut(s) 1389, 1396
PspN4I GGNNCC 8 cut(s) 6, 275, 594, 864, 1129, 1395, 1502, 1578
PspOMI GGGCCC 1 cut(s) 273
PspPI GGNCC 8 cut(s) 193, 273, 274, 592, 1025, 1127, 1208, 1685
PspPPI RGGWCCY 1 cut(s) 1127
PstI CTGCAG 1 cut(s) 712
PsyI GACNNNGTC 2 cut(s) 677, 1672
PvuII CAGCTG 2 cut(s) 242, 1550
RsaI GTAC 4 cut(s) 135, 1348, 1479, 1485
RsaNI GTAC 4 cut(s) 134, 1347, 1478, 1484
RseI CAYNNNNRTG 3 cut(s) 669, 857, 1564
SapI GCTCTTC 1 cut(s) 432
SaqAI TTAA 4 cut(s) 89, 624, 1121, 1223
SatI GCNGC 9 cut(s) 207, 240, 718, 1008, 1182, 1365, 1472, 1548, 1570
Sau3AI GATC 2 cut(s) 497, 1373
Sau96I GGNCC 8 cut(s) 193, 273, 274, 592, 1025, 1127, 1208, 1685
ScaI AGTACT 1 cut(s) 1348
SchI GAGTC 3 cut(s) 331, 902, 1663
ScrFI CCNGG 2 cut(s) 1391, 1398
SduI GDGCHC 2 cut(s) 143, 277
SfaNI GCATC 4 cut(s) 22, 1019, 1546, 1684
SfcI CTRYAG 1 cut(s) 708
SfuI TTCGAA 2 cut(s) 799, 1663
SinI GGWCC 4 cut(s) 592, 1025, 1127, 1208
SmiMI CAYNNNNRTG 3 cut(s) 669, 857, 1564
SmlI CTYRAG 1 cut(s) 88
SmoI CTYRAG 1 cut(s) 88
SpeI ACTAGT 1 cut(s) 1616
SsiI CCGC 4 cut(s) 277, 866, 1153, 1364
SspI AATATT 1 cut(s) 1326
SspMI CTAG 1 cut(s) 1617
StyD4I CCNGG 2 cut(s) 1389, 1396
StyI CCWWGG 3 cut(s) 585, 1309, 1580
TaaI ACNGT 9 cut(s) 184, 464, 519, 572, 763, 997, 1088, 1244, 1542
TaiI ACGT 1 cut(s) 1096
TaqI TCGA 3 cut(s) 324, 799, 1663
TaqII GACCGA 2 cut(s) 950, 1225
TatI WGTACW 3 cut(s) 133, 1346, 1477
TauI GCSGC 1 cut(s) 1367
TfiI GAWTC 5 cut(s) 575, 746, 1112, 1610, 1657
Tru1I TTAA 4 cut(s) 89, 624, 1121, 1223
Tru9I TTAA 4 cut(s) 89, 624, 1121, 1223
TscAI CASTG 5 cut(s) 111, 524, 1093, 1166, 1658
TseFI GTSAC 7 cut(s) 566, 647, 656, 1283, 1297, 1465, 1619
TseI GCWGC 8 cut(s) 206, 239, 717, 1007, 1181, 1471, 1547, 1569
Tsp45I GTSAC 7 cut(s) 566, 647, 656, 1283, 1297, 1465, 1619
TspDTI ATGAA 8 cut(s) 276, 330, 564, 621, 1212, 1347, 1373, 1628
TspRI CASTG 5 cut(s) 111, 524, 1093, 1166, 1658
Tth111I GACNNNGTC 2 cut(s) 677, 1672
Van91I CCANNNNNTGG 1 cut(s) 62
Vha464I CTTAAG 1 cut(s) 88
VpaK11BI GGWCC 4 cut(s) 592, 1025, 1127, 1208
XapI RAATTY 6 cut(s) 429, 729, 784, 801, 1267, 1401
XceI RCATGY 2 cut(s) 1305, 1466
XmnI GAANNNNTTC 2 cut(s) 220, 555
XspI CTAG 1 cut(s) 1617
ZrmI AGTACT 1 cut(s) 1348
Zsp2I ATGCAT 2 cut(s) 1510, 1699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.