Rorug05G0269700

NADH kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000832
Physical Location & Seq
Reverse (-)
30423797 .. 30427177
3381 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0269700.1

Sequence Viewer

Length: 2724 bp
ATGTTACAGGGCAACATCTTGCTTTGGAATTGCAGAGGCATAGTTAATGCTGAAACTCAGCGTGCCCTAGTTGACATTGTACTGGCTAAGAAACCAACGTTGATTTTTTTATCAGAAACCCTGGCACAAAAGGCTACGATTGAATCAATCACACGTCGTCTGGGCTTCAAGGACTGCTTCTGCGTCTCACAGGAGGAGAACTCGCAGGGCCTCGCCATTCTATGGACTGATGAGACTCACGTAAATGTGAGAAGTCATTTCCCAAACCACATAGATATGGAGATTGGACTATTGGGTTCTGATCTATGGCGTTTCACGGGCATTTACGGAGTTGCTGCACGGGGTGGAAGGGATCGAACTTGGCAGTTGATTGAAACCCTAGCTGCGCAGACCTGCCTGCTGCCATGGTTGATGGCGGGGGACTTCAATGAAGTGTTGGTGCGCAAGGACAAGTCTGGGGGGCCTCCTAGGGCTCTTGCGGGGATGGCCAAATTTAGAAGAACAATGACCCGGTGTGGTTTGAGTGACATGGGGTTCGTTGGAAGTCGTTTTACTTGGTCAAACAGGTATACAAAGGAGCGCCTCGATAGGGGTTTTCAAACTGTCCAATGGCGGCATCGTTTCCCATATAGTAGGGTGATAACCCTCAGTCCATCAGAGTCAGATCACTGTCCCTTGCTAATTGAGGTAAGCATTGCACCACAGATCCGACGGAGTTTCCATAGACCATTCCGGTTTGAAGAAGCATGGTTTGGTAAGGAAGATTGTTTGAACATTATAAAACAAGGCTGGGCCACGCCTACGACTGGTAATGGTCTACAACAACTTGGCAGCAAGACGAGGGAGTTAGGGATTCAGCTGAGTGCATGGCATCGAACTGAGTTCAACAGACAACAGAATGAGATGAAAGTGATCCAGGAAAAGCTAAAGGACTTAATGAGGCAACCCCATTCAGGTCAGGCATATGAGGAGCAAAAACTGTTGCACATTCAATATAGTCAACTGCTGTCCAATCAGGAGAAATATTGGAAGCAAAGGTCTCGGGCTTTGTGGTTGAAAGAGGGTGATAGGAATACTGCCTACTTTCACCGGAGAGCAAGTAATCCAAGGAATAGAAATATGATAAGAGGTTTGCTTGATGAGAACAATGTTTGGCAGGTCAATTTGGACGCACTAGAAAATGTGATAGCTGCTGTGCCACGCAAAGTAACAGATGAGATGAATAATGCTCTATTAGCTACATACACTAATGAGGAGATTAAAAACGCTCTACTCCAAATGCACCCGTCGAAAAGTCCTGGGCCGGATGCTCAATGGATTGATGTTATCATGCAATGCATTACCTCTGTGACTTATTCTATTCTCGTACAAGGGGAGCCCTCAGCTAAGATCACTCCTACACGTGGTAACCGACAAGATGATAGTATTCTCTTTGGCTCTGCCACAGAGGATGAATGTGTAAGGTACAGATTGATTCTTGATACTTATGAGCAGGCCTCAGGACAACAAGTTAATTTCCGGAAGAGTAGTGTTGTTTTCAGTAATAATGTGCAGGAGGCTCGACAACATCATTTGGCAACTATTTTGGGGGTGAATTGTGTCAAGGAACATGATAAGTATCTTGGGCTGCCTATAAGGGTGGGCAGGTCAAAAACTGACATCTTTGCATACATTAAAGAAAGGTTGACAAAAAAGTTGGTTAACTGGAAGGCGAAGATCCTAAGCTCGGCGGGAAAGGAAATTCTTATCAAAGCAGTGGCTCAAACCATGCCATTGTATGCCATGAACTGCCATTTGCTGCCAAAAACTTTATGTGATGACATTCATCAACTATGTGCATCTTTCTTTTGGGGAGACACTGATGACAAGAGAAAGATTCATTGGAGGAGTTGGGAGAAACTGTGCCTAACCAAACTAGAAGGTGGAATGGGTTCTAAAAACATCTATGCTTATAACTTAGCAATGCTTGGAAAACAAGGGTGGAGATTACTATCCAATCCTCAGTCGTTGATTGGTCAATTATACAAGGCCAAATATTTTCCTACTTGCTCTTTCTGGGAGGCAGAAGTTGGGGAATCTCCATCTTTCTCTTGGAGAAGTATTTTAAGTGCCAGACCACTGTTTAAAGCTGGGGTACAGTGGTGTATTGGAGATAGGACTCAAGTCAGCATTTGGAATGATAGGTGGATACCAATGTGCCCACAATATTTGATCCACAAACCTCCTGATTGTGTCTTTGAGCTTGTCTCGGATCTAATTGACGCACATACGAGGCAATGGATTCCAGAGGCAATTCACACAATCTTTCCACCTGACATAGCTAAGAAGGTACTTAGCATTACATTGAGTCGTCAGATTAGGCGGGATAAACTGTGCTGGAGTCCGGAGAAAAAGGGATGTTATTTTGTTAAGACGGCTTACTGGATTGCCCGCACTAAGGTGCTAGAAAATGTGCTTGTTTCTACCTCTCATGGTAATCCTTTTCTAGAGTTGTGGAGAAGACTTTGGCAGGTTAACGTACCAGGGAAAGTGAAGATTTGTGTGTGGAGGGCATGCTCTAATTTGCTTCCTACAAGGGCTAAACTTACCTCTAAGGGATATGAGGGTGACCTTCGCTGCCTCGTGTGTTCCCATCCATATGAGGACACATCTCACCTCTTTTGTAAGTGCCCTACTGCCGTTGATATACTCACAGCACCTCCTTTTAACTCTGGAAGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

907

Amino Acids

104.13

Weight (kDa)

9.34

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 8 - 197 6.6e-07 Endonuclease/Exonuclease/phosphatase family
zf-RVT PF13966 829 - 894 3.2e-14 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G21070 AT3G21070 AT3G21070 AT3G21070
fragaria_vesca FvH4_3g28510
malus_domestica MD03G1172500.v1.1 MD11G1190400.v1.1
prunus_persica Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1
pyrus_communis pycom03g12970 pycom11g16670
rosa_chinensis RchiOBHm_Chr5g0052801
rosa_laevigata RLG00000034795
rosa_multiflora Rmu_sc0004335.1_g000006 Rmu_sc0032421.1_g000001 Rmu_ssc0000311.1_g000025
rosa_roxburghii Rroxscaffold_1G00028620 Rroxscaffold_4G00318540
rosa_rugosa Rorug05G0269700 Rorug05G0269800
rosa_samantha Rh5AG341600 Rh5BG352300 Rh5CG379000 Rh5DG368700
rosa_wichuraiana Rw5G032210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 779, 1953
Acc16I TGCGCA 2 cut(s) 387, 443
Acc36I ACCTGC 4 cut(s) 401, 1147, 1635, 2500
AccB7I CCANNNNNTGG 1 cut(s) 222
AccI GTMKAC 2 cut(s) 569, 817
AccIII TCCGGA 2 cut(s) 1518, 2383
AciI CCGC 6 cut(s) 416, 479, 613, 1730, 2362, 2431
AclI AACGTT 1 cut(s) 98
AclWI GGATC 6 cut(s) 360, 700, 907, 1711, 2206, 2259
AcoI YGGCCR 1 cut(s) 486
AcsI RAATTY 2 cut(s) 491, 1740
AcvI CACGTG 1 cut(s) 1403
AdeI CACNNNGTG 1 cut(s) 344
AfaI GTAC 6 cut(s) 81, 1368, 1466, 2136, 2331, 2520
AfiI CCNNNNNNNGG 7 cut(s) 222, 589, 806, 953, 1403, 1726, 2437
AflIII ACRYGT 2 cut(s) 152, 1400
AjiI CACGTC 1 cut(s) 155
AjnI CCWGG 4 cut(s) 120, 915, 1297, 2521
AjuI GAANNNNNNNTTGG 4 cut(s) 735, 767, 1864, 1896
AleI CACNNNNGTG 1 cut(s) 2438
Alw26I GTCTC 5 cut(s) 190, 227, 1044, 1848, 2251
AlwI GGATC 6 cut(s) 360, 700, 907, 1711, 2206, 2259
Ama87I CYCGRG 1 cut(s) 1041
Aor13HI TCCGGA 2 cut(s) 1518, 2383
AoxI GGCC 7 cut(s) 208, 461, 486, 792, 1301, 1494, 2028
ApeKI GCWGC 8 cut(s) 335, 383, 400, 831, 1190, 1627, 1798, 2616
ApoI RAATTY 2 cut(s) 491, 1740
ArsI GACNNNNNNTTYG 2 cut(s) 518, 550
Asp700I GAANNNNTTC 1 cut(s) 1930
AspA2I CCTAGG 1 cut(s) 467
AspLEI GCGC 3 cut(s) 388, 444, 582
AspS9I GGNCC 4 cut(s) 208, 461, 792, 1301
AsuC2I CCSGG 1 cut(s) 511
AsuHPI GGTGA 6 cut(s) 649, 1076, 1079, 1603, 2618, 2645
AvaI CYCGRG 1 cut(s) 1041
AvrII CCTAGG 1 cut(s) 467
AxyI CCTNAGG 1 cut(s) 1498
BaeGI GKGCMC 3 cut(s) 67, 2201, 2672
BaeI ACNNNNGTAYC 4 cut(s) 2126, 2159, 2180, 2213
BalI TGGCCA 1 cut(s) 488
BanII GRGCYC 2 cut(s) 475, 1380
BarI GAAGNNNNNNTAC 2 cut(s) 535, 567
BauI CACGAG 1 cut(s) 2621
BbrPI CACGTG 1 cut(s) 1403
BbsI GAAGAC 1 cut(s) 2506
BbvCI CCTCAGC 1 cut(s) 1381
BbvI GCAGC 8 cut(s) 322, 370, 387, 843, 1177, 1614, 1785, 2603
BccI CCATC 5 cut(s) 406, 478, 661, 2089, 2640
BceAI ACGGC 2 cut(s) 2430, 2663
BcgI CGANNNNNNTGC 4 cut(s) 1022, 1056, 1541, 1575
BciT130I CCWGG 4 cut(s) 122, 917, 1299, 2523
BciVI GTATCC 1 cut(s) 2181
BcnI CCSGG 1 cut(s) 511
BcoDI GTCTC 5 cut(s) 190, 227, 1044, 1848, 2251
BfaI CTAG 7 cut(s) 68, 380, 468, 1175, 1916, 2444, 2486
BfoI RGCGCY 1 cut(s) 583
BfuAI ACCTGC 4 cut(s) 401, 1147, 1635, 2500
BfuI GTATCC 1 cut(s) 2181
BisI GCNGC 9 cut(s) 336, 384, 401, 614, 832, 1191, 1628, 1799, 2617
BlnI CCTAGG 1 cut(s) 467
BlsI GCNGC 9 cut(s) 337, 385, 402, 615, 833, 1192, 1629, 1800, 2618
Bme1390I CCNGG 5 cut(s) 122, 511, 917, 1299, 2523
BmeT110I CYCGRG 1 cut(s) 1041
BmgBI CACGTC 1 cut(s) 155
BmgT120I GGNCC 4 cut(s) 208, 461, 792, 1301
BmiI GGNNCC 2 cut(s) 462, 1377
BmrFI CCNGG 5 cut(s) 122, 511, 917, 1299, 2523
BmsI GCATC 4 cut(s) 625, 880, 1297, 1847
BoxI GACNNNNGTC 1 cut(s) 2162
BpiI GAAGAC 1 cut(s) 2506
BplI GAGNNNNNCTC 6 cut(s) 185, 217, 1481, 1513, 2231, 2263
BpmI CTGGAG 1 cut(s) 2398
Bpu10I CCTNAGC 2 cut(s) 1381, 1721
BpuEI CTTGAG 1 cut(s) 2145
BpuMI CCSGG 1 cut(s) 511
BsaAI YACGTR 2 cut(s) 241, 1403
BsaBI GATNNNNATC 2 cut(s) 1617, 1990
BsaI GGTCTC 1 cut(s) 1044
BsaJI CCNNGG 6 cut(s) 120, 404, 467, 1106, 1298, 2522
BsaWI WCCGGW 4 cut(s) 732, 1089, 1518, 2383
BsaXI ACNNNNNCTCC 6 cut(s) 1084, 1114, 1886, 1916, 2683, 2713
Bsc4I CCNNNNNNNGG 7 cut(s) 222, 589, 806, 953, 1403, 1726, 2437
Bse1I ACTGG 4 cut(s) 87, 811, 1709, 2426
Bse21I CCTNAGG 1 cut(s) 1498
Bse3DI GCAATG 4 cut(s) 693, 1340, 1968, 2282
Bse8I GATNNNNATC 2 cut(s) 1617, 1990
BseAI TCCGGA 2 cut(s) 1518, 2383
BseBI CCWGG 4 cut(s) 122, 917, 1299, 2523
BseDI CCNNGG 6 cut(s) 120, 404, 467, 1106, 1298, 2522
BseGI GGATG 5 cut(s) 489, 1312, 1456, 2402, 2632
BseJI GATNNNNATC 2 cut(s) 1617, 1990
BseLI CCNNNNNNNGG 7 cut(s) 222, 589, 806, 953, 1403, 1726, 2437
BseMI GCAATG 4 cut(s) 693, 1340, 1968, 2282
BseMII CTCAG 7 cut(s) 71, 661, 851, 870, 1395, 1512, 2015
BseNI ACTGG 4 cut(s) 87, 811, 1709, 2426
BseRI GAGGAG 4 cut(s) 209, 983, 1268, 1900
BseSI GKGCMC 3 cut(s) 67, 2201, 2672
BseXI GCAGC 8 cut(s) 322, 370, 387, 843, 1177, 1614, 1785, 2603
BseYI CCCAGC 2 cut(s) 789, 2129
BsgI GTGCAG 2 cut(s) 321, 1571
BshFI GGCC 7 cut(s) 210, 463, 488, 794, 1303, 1496, 2030
BsiHKCI CYCGRG 1 cut(s) 1041
BsiSI CCGG 6 cut(s) 511, 733, 1090, 1304, 1519, 2384
BslFI GGGAC 2 cut(s) 434, 657
BslI CCNNNNNNNGG 7 cut(s) 222, 589, 806, 953, 1403, 1726, 2437
BsmAI GTCTC 5 cut(s) 190, 227, 1044, 1848, 2251
BsmBI CGTCTC 1 cut(s) 190
BsmFI GGGAC 2 cut(s) 434, 657
BsnI GGCC 7 cut(s) 210, 463, 488, 794, 1303, 1496, 2030
Bso31I GGTCTC 1 cut(s) 1044
BsoBI CYCGRG 1 cut(s) 1041
Bsp1286I GDGCHC 5 cut(s) 67, 475, 1380, 2201, 2672
Bsp13I TCCGGA 2 cut(s) 1518, 2383
Bsp143I GATC 9 cut(s) 301, 352, 664, 705, 912, 1389, 1716, 2211, 2251
Bsp19I CCATGG 1 cut(s) 404
BspACI CCGC 6 cut(s) 416, 479, 613, 1730, 2362, 2431
BspANI GGCC 7 cut(s) 210, 463, 488, 794, 1303, 1496, 2030
BspCNI CTCAG 7 cut(s) 70, 660, 852, 871, 1394, 1511, 2014
BspEI TCCGGA 2 cut(s) 1518, 2383
BspLI GGNNCC 2 cut(s) 462, 1377
BspMI ACCTGC 4 cut(s) 401, 1147, 1635, 2500
BspPI GGATC 6 cut(s) 360, 700, 907, 1711, 2206, 2259
BspTNI GGTCTC 1 cut(s) 1044
BsrDI GCAATG 4 cut(s) 693, 1340, 1968, 2282
BsrI ACTGG 4 cut(s) 87, 811, 1709, 2426
BssECI CCNNGG 6 cut(s) 120, 404, 467, 1106, 1298, 2522
BssMI GATC 9 cut(s) 301, 352, 664, 705, 912, 1389, 1716, 2211, 2251
BssNAI GTATAC 1 cut(s) 570
BssSI CACGAG 1 cut(s) 2621
BssT1I CCWWGG 3 cut(s) 404, 467, 1106
Bst1107I GTATAC 1 cut(s) 570
Bst2BI CACGAG 1 cut(s) 2621
Bst2UI CCWGG 4 cut(s) 122, 917, 1299, 2523
Bst4CI ACNGT 7 cut(s) 604, 671, 981, 1902, 2121, 2139, 2373
Bst6I CTCTTC 1 cut(s) 1517
BstBAI YACGTR 2 cut(s) 241, 1403
BstC8I GCNNGC 5 cut(s) 63, 398, 1494, 2431, 2554
BstDSI CCRYGG 1 cut(s) 404
BstEII GGTNACC 2 cut(s) 1406, 2606
BstF5I GGATG 5 cut(s) 489, 1312, 1456, 2402, 2632
BstH2I RGCGCY 1 cut(s) 583
BstHHI GCGC 3 cut(s) 388, 444, 582
BstKTI GATC 9 cut(s) 304, 355, 667, 708, 915, 1392, 1719, 2214, 2254
BstMAI GTCTC 5 cut(s) 190, 227, 1044, 1848, 2251
BstMBI GATC 9 cut(s) 301, 352, 664, 705, 912, 1389, 1716, 2211, 2251
BstMWI GCNNNNNNNGC 3 cut(s) 131, 485, 1235
BstNI CCWGG 4 cut(s) 122, 917, 1299, 2523
BstNSI RCATGY 1 cut(s) 2556
BstPAI GACNNNNGTC 1 cut(s) 2162
BstPI GGTNACC 2 cut(s) 1406, 2606
BstSCI CCNGG 5 cut(s) 120, 509, 915, 1297, 2521
BstSLI GKGCMC 3 cut(s) 67, 2201, 2672
BstV1I GCAGC 8 cut(s) 322, 370, 387, 843, 1177, 1614, 1785, 2603
BstV2I GAAGAC 1 cut(s) 2506
BstX2I RGATCY 3 cut(s) 705, 1716, 2251
BstYI RGATCY 3 cut(s) 705, 1716, 2251
BstZ17I GTATAC 1 cut(s) 570
Bsu36I CCTNAGG 1 cut(s) 1498
BsuI GTATCC 1 cut(s) 2181
BsuRI GGCC 7 cut(s) 210, 463, 488, 794, 1303, 1496, 2030
BtgI CCRYGG 1 cut(s) 404
BtrI CACGTC 1 cut(s) 155
BtsCI GGATG 5 cut(s) 489, 1312, 1456, 2402, 2632
BtsI GCAGTG 1 cut(s) 1761
BtsIMutI CAGTG 5 cut(s) 667, 1761, 1857, 2117, 2144
BveI ACCTGC 4 cut(s) 401, 1147, 1635, 2500
Cac8I GCNNGC 5 cut(s) 63, 398, 1494, 2431, 2554
CfoI GCGC 3 cut(s) 388, 444, 582
Cfr13I GGNCC 4 cut(s) 208, 461, 792, 1301
CseI GACGC 3 cut(s) 172, 1178, 2270
Csp6I GTAC 6 cut(s) 80, 1367, 1465, 2135, 2330, 2519
CspCI CAANNNNNGTGG 2 cut(s) 2190, 2225
CviQI GTAC 6 cut(s) 80, 1367, 1465, 2135, 2330, 2519
DpnI GATC 9 cut(s) 303, 354, 666, 707, 914, 1391, 1718, 2213, 2253
DpnII GATC 9 cut(s) 301, 352, 664, 705, 912, 1389, 1716, 2211, 2251
DraI TTTAAA 1 cut(s) 2125
DraIII CACNNNGTG 1 cut(s) 344
EaeI YGGCCR 1 cut(s) 486
Eam1104I CTCTTC 1 cut(s) 1517
EarI CTCTTC 1 cut(s) 1517
Eco130I CCWWGG 3 cut(s) 404, 467, 1106
Eco147I AGGCCT 1 cut(s) 1496
Eco24I GRGCYC 2 cut(s) 475, 1380
Eco31I GGTCTC 1 cut(s) 1044
Eco72I CACGTG 1 cut(s) 1403
Eco81I CCTNAGG 1 cut(s) 1498
Eco88I CYCGRG 1 cut(s) 1041
Eco91I GGTNACC 2 cut(s) 1406, 2606
EcoO109I RGGNCCY 2 cut(s) 208, 461
EcoO65I GGTNACC 2 cut(s) 1406, 2606
EcoRII CCWGG 4 cut(s) 120, 915, 1297, 2521
EcoT14I CCWWGG 3 cut(s) 404, 467, 1106
EcoT22I ATGCAT 1 cut(s) 1340
EcoT38I GRGCYC 2 cut(s) 475, 1380
ErhI CCWWGG 3 cut(s) 404, 467, 1106
Esp3I CGTCTC 1 cut(s) 190
FalI AAGNNNNNCTT 2 cut(s) 161, 193
FaqI GGGAC 2 cut(s) 434, 657
FauI CCCGC 5 cut(s) 409, 472, 1723, 2355, 2438
FauNDI CATATG 2 cut(s) 964, 2638
FblI GTMKAC 2 cut(s) 569, 817
Fnu4HI GCNGC 9 cut(s) 336, 384, 401, 614, 832, 1191, 1628, 1799, 2617
FokI GGATG 5 cut(s) 496, 1319, 1463, 2409, 2619
FriOI GRGCYC 2 cut(s) 475, 1380
Fsp4HI GCNGC 9 cut(s) 336, 384, 401, 614, 832, 1191, 1628, 1799, 2617
FspBI CTAG 7 cut(s) 68, 380, 468, 1175, 1916, 2444, 2486
FspI TGCGCA 2 cut(s) 387, 443
GlaI GCGC 3 cut(s) 387, 443, 581
GluI GCNGC 9 cut(s) 336, 384, 401, 614, 832, 1191, 1628, 1799, 2617
GsaI CCCAGC 2 cut(s) 793, 2133
GsuI CTGGAG 1 cut(s) 2398
HaeII RGCGCY 1 cut(s) 583
HaeIII GGCC 7 cut(s) 210, 463, 488, 794, 1303, 1496, 2030
HapII CCGG 6 cut(s) 511, 733, 1090, 1304, 1519, 2384
HgaI GACGC 3 cut(s) 172, 1178, 2270
HhaI GCGC 3 cut(s) 388, 444, 582
Hin6I GCGC 3 cut(s) 386, 442, 580
HinP1I GCGC 3 cut(s) 386, 442, 580
HincII GTYRAC 5 cut(s) 73, 1001, 1686, 1702, 2515
HindII GTYRAC 5 cut(s) 73, 1001, 1686, 1702, 2515
HpaI GTTAAC 2 cut(s) 1702, 2515
HpaII CCGG 6 cut(s) 511, 733, 1090, 1304, 1519, 2384
HphI GGTGA 6 cut(s) 649, 1076, 1079, 1603, 2618, 2645
Hpy166II GTNNAC 7 cut(s) 73, 570, 818, 1001, 1686, 1702, 2515
Hpy188I TCNGA 7 cut(s) 115, 301, 658, 664, 710, 2251, 2355
Hpy188III TCNNGA 9 cut(s) 1016, 1478, 1500, 1519, 2225, 2285, 2384, 2486, 2712
Hpy8I GTNNAC 7 cut(s) 73, 570, 818, 1001, 1686, 1702, 2515
Hpy99I CGWCG 3 cut(s) 159, 714, 1291
HpyAV CCTTC 5 cut(s) 342, 1702, 1913, 2320, 2621
HpyCH4III ACNGT 7 cut(s) 604, 671, 981, 1902, 2121, 2139, 2373
HpyCH4IV ACGT 5 cut(s) 98, 154, 240, 1402, 2517
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 485, 1235
HpySE526I ACGT 5 cut(s) 98, 154, 240, 1402, 2517
HspAI GCGC 3 cut(s) 386, 442, 580
Kpn2I TCCGGA 2 cut(s) 1518, 2383
KspAI GTTAAC 2 cut(s) 1702, 2515
Kzo9I GATC 9 cut(s) 301, 352, 664, 705, 912, 1389, 1716, 2211, 2251
LmnI GCTCC 3 cut(s) 577, 970, 1375
Lsp1109I GCAGC 8 cut(s) 322, 370, 387, 843, 1177, 1614, 1785, 2603
LweI GCATC 4 cut(s) 625, 880, 1297, 1847
MaeI CTAG 7 cut(s) 68, 380, 468, 1175, 1916, 2444, 2486
MaeII ACGT 5 cut(s) 98, 154, 240, 1402, 2517
MaeIII GTNAC 6 cut(s) 3, 524, 1207, 1348, 1406, 2606
MalI GATC 9 cut(s) 303, 354, 666, 707, 914, 1391, 1718, 2213, 2253
MboI GATC 9 cut(s) 301, 352, 664, 705, 912, 1389, 1716, 2211, 2251
MboII GAAGA 7 cut(s) 510, 752, 773, 1534, 1726, 2511, 2545
MflI RGATCY 3 cut(s) 705, 1716, 2251
MhlI GDGCHC 5 cut(s) 67, 475, 1380, 2201, 2672
MlsI TGGCCA 1 cut(s) 488
MluNI TGGCCA 1 cut(s) 488
MlyI GAGTC 5 cut(s) 229, 668, 2152, 2356, 2389
MmeI TCCRAC 2 cut(s) 520, 733
Mox20I TGGCCA 1 cut(s) 488
Mph1103I ATGCAT 1 cut(s) 1340
MroI TCCGGA 2 cut(s) 1518, 2383
MroXI GAANNNNTTC 1 cut(s) 1930
MscI TGGCCA 1 cut(s) 488
MslI CAYNNNNRTG 4 cut(s) 243, 275, 2438, 2637
Msp20I TGGCCA 1 cut(s) 488
MspA1I CMGCKG 1 cut(s) 859
MspI CCGG 6 cut(s) 511, 733, 1090, 1304, 1519, 2384
MspR9I CCNGG 5 cut(s) 122, 511, 917, 1299, 2523
MvaI CCWGG 4 cut(s) 122, 917, 1299, 2523
MwoI GCNNNNNNNGC 3 cut(s) 131, 485, 1235
NciI CCSGG 1 cut(s) 511
NcoI CCATGG 1 cut(s) 404
NdeI CATATG 2 cut(s) 964, 2638
NdeII GATC 9 cut(s) 301, 352, 664, 705, 912, 1389, 1716, 2211, 2251
NlaIV GGNNCC 2 cut(s) 462, 1377
NmeAIII GCCGAG 1 cut(s) 1706
NmuCI GTSAC 3 cut(s) 524, 1348, 2606
NsbI TGCGCA 2 cut(s) 387, 443
NsiI ATGCAT 1 cut(s) 1340
NspI RCATGY 1 cut(s) 2556
OliI CACNNNNGTG 1 cut(s) 2438
PaeI GCATGC 1 cut(s) 2556
PceI AGGCCT 1 cut(s) 1496
PcsI WCGNNNNNNNCGW 1 cut(s) 1408
PdmI GAANNNNTTC 1 cut(s) 1930
PfeI GAWTC 6 cut(s) 143, 853, 1474, 1876, 2075, 2281
PflMI CCANNNNNTGG 1 cut(s) 222
PfoI TCCNGGA 1 cut(s) 915
PkrI GCNGC 9 cut(s) 337, 385, 402, 615, 833, 1192, 1629, 1800, 2618
PleI GAGTC 5 cut(s) 229, 667, 2152, 2355, 2388
PmaCI CACGTG 1 cut(s) 1403
PmlI CACGTG 1 cut(s) 1403
PpsI GAGTC 5 cut(s) 229, 667, 2152, 2355, 2388
Ppu21I YACGTR 2 cut(s) 241, 1403
PshAI GACNNNNGTC 1 cut(s) 2162
PsiI TTATAA 2 cut(s) 779, 1953
Psp1406I AACGTT 1 cut(s) 98
Psp6I CCWGG 4 cut(s) 120, 915, 1297, 2521
PspCI CACGTG 1 cut(s) 1403
PspEI GGTNACC 2 cut(s) 1406, 2606
PspFI CCCAGC 2 cut(s) 789, 2129
PspGI CCWGG 4 cut(s) 120, 915, 1297, 2521
PspN4I GGNNCC 2 cut(s) 462, 1377
PspPI GGNCC 4 cut(s) 208, 461, 792, 1301
PsuI RGATCY 3 cut(s) 705, 1716, 2251
PvuII CAGCTG 1 cut(s) 859
RsaI GTAC 6 cut(s) 81, 1368, 1466, 2136, 2331, 2520
RsaNI GTAC 6 cut(s) 80, 1367, 1465, 2135, 2330, 2519
RseI CAYNNNNRTG 4 cut(s) 243, 275, 2438, 2637
SatI GCNGC 9 cut(s) 336, 384, 401, 614, 832, 1191, 1628, 1799, 2617
Sau3AI GATC 9 cut(s) 301, 352, 664, 705, 912, 1389, 1716, 2211, 2251
Sau96I GGNCC 4 cut(s) 208, 461, 792, 1301
SchI GAGTC 5 cut(s) 229, 668, 2152, 2356, 2389
ScrFI CCNGG 5 cut(s) 122, 511, 917, 1299, 2523
SduI GDGCHC 5 cut(s) 67, 475, 1380, 2201, 2672
SfaNI GCATC 4 cut(s) 625, 880, 1297, 1847
SmiMI CAYNNNNRTG 4 cut(s) 243, 275, 2438, 2637
SmlI CTYRAG 1 cut(s) 2160
SmoI CTYRAG 1 cut(s) 2160
SphI GCATGC 1 cut(s) 2556
SseBI AGGCCT 1 cut(s) 1496
SsiI CCGC 6 cut(s) 416, 479, 613, 1730, 2362, 2431
SspI AATATT 3 cut(s) 1025, 2036, 2207
SspMI CTAG 7 cut(s) 68, 380, 468, 1175, 1916, 2444, 2486
StuI AGGCCT 1 cut(s) 1496
StyD4I CCNGG 5 cut(s) 120, 509, 915, 1297, 2521
StyI CCWWGG 3 cut(s) 404, 467, 1106
TaaI ACNGT 7 cut(s) 604, 671, 981, 1902, 2121, 2139, 2373
TaiI ACGT 5 cut(s) 101, 157, 243, 1405, 2520
TaqI TCGA 5 cut(s) 355, 585, 874, 1289, 1561
TatI WGTACW 1 cut(s) 79
TauI GCSGC 1 cut(s) 616
TfiI GAWTC 6 cut(s) 143, 853, 1474, 1876, 2075, 2281
TscAI CASTG 5 cut(s) 674, 1761, 1864, 2124, 2144
TseFI GTSAC 3 cut(s) 524, 1348, 2606
TseI GCWGC 8 cut(s) 335, 383, 400, 831, 1190, 1627, 1798, 2616
Tsp45I GTSAC 3 cut(s) 524, 1348, 2606
TspDTI ATGAA 7 cut(s) 444, 920, 1235, 1467, 1799, 1814, 1868
TspGWI ACGGA 2 cut(s) 342, 727
TspRI CASTG 5 cut(s) 674, 1761, 1864, 2124, 2144
Van91I CCANNNNNTGG 1 cut(s) 222
XapI RAATTY 2 cut(s) 491, 1740
XbaI TCTAGA 1 cut(s) 2485
XceI RCATGY 1 cut(s) 2556
XcmI CCANNNNNNNNNTGG 1 cut(s) 2088
XmaJI CCTAGG 1 cut(s) 467
XmiI GTMKAC 2 cut(s) 569, 817
XmnI GAANNNNTTC 1 cut(s) 1930
XspI CTAG 7 cut(s) 68, 380, 468, 1175, 1916, 2444, 2486
Zsp2I ATGCAT 1 cut(s) 1340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.