RchiOBHm_Chr5g0052801

NADH kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000832
Physical Location & Seq
Reverse (-)
54899471 .. 54909868
10398 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33017

Sequence Viewer

Length: 1563 bp
ATGGCTCCCAGCAAGTCCAATTCTTCAGGTGATGCAAGTGTTTCATGCCCACAACCAGAGAATGGTTGTCTCAATTCACTTTCCCTCTTAAGTTCAGAGAAGGCAGTCGAGGAACTTCTCGAGCAATCTTCACTTCAGGAAACAGATGACCACCTCATAGAGTTCTCAGAGGCTATGAGAACGGTTGCAAAGGCCCTGAGACGAGCTGCCGAAGGAAAGGCTTCTGCACAAGCTGAAGCAGCTGAATGGAAGCGCAAATATGAACTGGAGAGGGCGCGCAATCTGCATCTGGAACCTAAAGGGCCATCACATAGAGAGAATAACGGGGATGATGAGAGGATAGAGAACTTGGCCAGGAGATCAAATGACCAATCTGAACAATGTTGTGGGAGTAATGGGATATGCTCTCATGAAGTTCTTCGGGATGGTGAATGTGATACTGATTCAAAATCGCTTCTCAATAAGTTTGCAAGAAAGGCGTCATTCAAACTTTCATGGTGCTGCAAAGGTGACCAGAGTGACCAGCACAAGCATGACATTGTCTCTTTTGAAAGAGGAAACATAACAACTGCAGAGCGCAGCAGTAAACAGATTTCTTTGAAGTGGGAAACTCACCCACGGACTGTGCTTATATTGACCAAACCAAATTCAACTTCTGTTCGAATTCTGTGTGCAGAAATGGTCAGATGGTTGAGAGAGCAGAAAAAGTTAGATATTTATGTGGAGCCACGTGTGAGAGTTGAACTTGTGACAGAGTCATCATACTACAACTTCGTCCAGACATGGAAAGAAGACACTGAGATTATGCTCCTGCATACAAAAGTTGACCTTGTGGTAACTCTTGGTGGGGATGGAACTGTCCTCTGGGCAGCATCCATGTTCAAAGGACCAGTTCCTCCTGTTGTGCCATTTTCTTTAGGATCTTTGGGATTTATGACTCCATTTCACAGTGAACAGTACCGGGATTATCTTGATTCACTTTTAAAAGGTCCCATTAGTATCACGCTACGGCACCGGTTGCAGTGCCATGTTATTAGAGAATCAGCTAAAAATGAGTACGAAACTGAAGGGCCAATACTTGTTTTAAATGAGGTTACAATTGACCGTGGGATATCATCATATCTCACAAATTTGGAATGCTATTGTGACAACTCTTTTGTCACATGCGTCCAAGGAGATGGGCTGATATTATCGACAACATCTGGCAGTACTGCGTATTCATTAGCAGCTGGAGGATCAATGGTCCATCCCCAGGTTCCAGGCATCCTCTTTACCCCTATTTGTCCTCATTCCCTATCGTTCCGGCCTCTGATATTACCTGAGTATGTGACATTGCGAGTACAAGTACCTTTTAACAGCAGAAGCCATGCATGGGCATCATTTGATGGGAAGGACAGGAAACGGCTGGCAGCTGGTGATGCACTCGTGTGCAGTATGGCTACTTGGCCCGTCCCCACTGCATGTCAAGTTGATTCAACCAGTGATTTCTTAACTAGCATTCATGATGGCCTGCACTGGAATCTGAGAAAGACTCAATCTTATGATGGCCCAAGGGATATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

520

Amino Acids

58.01

Weight (kDa)

6.01

Isoelectric Point (pI)

46.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_kinase PF01513 209 - 330 1.2e-18 ATP-NAD kinase N-terminal domain
NAD_kinase_C PF20143 362 - 477 3.2e-30 ATP-NAD kinase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G21070 AT3G21070 AT3G21070 AT3G21070
fragaria_vesca FvH4_3g28510
malus_domestica MD03G1172500.v1.1 MD11G1190400.v1.1
prunus_persica Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1
pyrus_communis pycom03g12970 pycom11g16670
rosa_chinensis RchiOBHm_Chr5g0052801
rosa_laevigata RLG00000034795
rosa_multiflora Rmu_sc0004335.1_g000006 Rmu_sc0032421.1_g000001 Rmu_ssc0000311.1_g000025
rosa_roxburghii Rroxscaffold_1G00028620 Rroxscaffold_4G00318540
rosa_rugosa Rorug05G0269700 Rorug05G0269800
rosa_samantha Rh5AG341600 Rh5BG352300 Rh5CG379000 Rh5DG368700
rosa_wichuraiana Rw5G032210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1011
AccB7I CCANNNNNTGG 1 cut(s) 62
AccII CGCG 1 cut(s) 277
AclWI GGATC 2 cut(s) 928, 1243
AcoI YGGCCR 1 cut(s) 351
AcsI RAATTY 3 cut(s) 646, 663, 1129
AcuI CTGAAG 4 cut(s) 9, 119, 255, 1086
AcvI CACGTG 1 cut(s) 731
AcyI GRCGYC 1 cut(s) 479
AfaI GTAC 5 cut(s) 959, 1058, 1210, 1341, 1347
AfiI CCNNNNNNNGG 3 cut(s) 62, 1252, 1372
AflII CTTAAG 1 cut(s) 88
AflIII ACRYGT 1 cut(s) 730
AgeI ACCGGT 1 cut(s) 1014
AgsI TTSAA 8 cut(s) 447, 487, 551, 601, 651, 743, 883, 1476
AjnI CCWGG 3 cut(s) 353, 1251, 1258
AjuI GAANNNNNNNTTGG 2 cut(s) 637, 669
AleI CACNNNNGTG 1 cut(s) 1426
AluBI AGCT 6 cut(s) 206, 233, 242, 1046, 1229, 1412
AluI AGCT 6 cut(s) 206, 233, 242, 1046, 1229, 1412
Alw26I GTCTC 3 cut(s) 74, 193, 547
AlwI GGATC 2 cut(s) 928, 1243
Ama87I CYCGRG 1 cut(s) 119
AoxI GGCC 8 cut(s) 192, 302, 351, 1070, 1304, 1445, 1507, 1546
ApeKI GCWGC 7 cut(s) 206, 239, 501, 579, 869, 1226, 1409
ApoI RAATTY 3 cut(s) 646, 663, 1129
ArsI GACNNNNNNTTYG 2 cut(s) 1139, 1171
AsiGI ACCGGT 1 cut(s) 1014
Asp700I GAANNNNTTC 2 cut(s) 220, 417
AspLEI GCGC 4 cut(s) 255, 277, 279, 579
AspS9I GGNCC 8 cut(s) 193, 302, 887, 989, 1070, 1243, 1446, 1547
AsuC2I CCSGG 1 cut(s) 962
AsuHPI GGTGA 5 cut(s) 41, 440, 521, 605, 1427
AsuII TTCGAA 1 cut(s) 661
AvaI CYCGRG 1 cut(s) 119
AvaII GGWCC 3 cut(s) 887, 989, 1243
BalI TGGCCA 1 cut(s) 353
BanI GGYRCC 1 cut(s) 1011
BarI GAAGNNNNNNTAC 2 cut(s) 1059, 1091
BauI CACGAG 1 cut(s) 1424
BbrPI CACGTG 1 cut(s) 731
BbsI GAAGAC 1 cut(s) 798
BbvI GCAGC 7 cut(s) 193, 251, 488, 591, 881, 1238, 1421
BccI CCATC 9 cut(s) 313, 419, 681, 845, 1172, 1254, 1379, 1499, 1538
BceAI ACGGC 2 cut(s) 1025, 1418
BciT130I CCWGG 3 cut(s) 355, 1253, 1260
BcnI CCSGG 1 cut(s) 962
BcoDI GTCTC 3 cut(s) 74, 193, 547
BfaI CTAG 1 cut(s) 1494
BfmI CTRYAG 1 cut(s) 570
BfrI CTTAAG 1 cut(s) 88
BisI GCNGC 7 cut(s) 207, 240, 502, 580, 870, 1227, 1410
BlsI GCNGC 7 cut(s) 208, 241, 503, 581, 871, 1228, 1411
BmcAI AGTACT 1 cut(s) 1210
Bme1390I CCNGG 4 cut(s) 355, 962, 1253, 1260
Bme18I GGWCC 3 cut(s) 887, 989, 1243
BmeT110I CYCGRG 1 cut(s) 119
BmgT120I GGNCC 8 cut(s) 193, 302, 887, 989, 1070, 1243, 1446, 1547
BmiI GGNNCC 6 cut(s) 6, 294, 726, 991, 1013, 1257
BmrFI CCNGG 4 cut(s) 355, 962, 1253, 1260
BmsI GCATC 6 cut(s) 22, 295, 881, 1272, 1385, 1408
BpiI GAAGAC 1 cut(s) 798
BplI GAGNNNNNCTC 2 cut(s) 1516, 1548
BpmI CTGGAG 2 cut(s) 287, 1251
Bpu14I TTCGAA 1 cut(s) 661
BpuMI CCSGG 1 cut(s) 962
BsaAI YACGTR 1 cut(s) 731
BsaHI GRCGYC 1 cut(s) 479
BsaJI CCNNGG 5 cut(s) 617, 1105, 1171, 1251, 1550
BsaWI WCCGGW 1 cut(s) 1014
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 1252, 1372
Bse118I RCCGGY 1 cut(s) 1014
Bse1I ACTGG 4 cut(s) 270, 890, 1479, 1520
Bse3DI GCAATG 1 cut(s) 1331
BseBI CCWGG 3 cut(s) 355, 1253, 1260
BseDI CCNNGG 5 cut(s) 617, 1105, 1171, 1251, 1550
BseGI GGATG 6 cut(s) 334, 430, 856, 872, 1246, 1263
BseLI CCNNNNNNNGG 3 cut(s) 62, 1252, 1372
BseMI GCAATG 1 cut(s) 1331
BseMII CTCAG 5 cut(s) 180, 188, 789, 1311, 1514
BseNI ACTGG 4 cut(s) 270, 890, 1479, 1520
BsePI GCGCGC 1 cut(s) 275
BseXI GCAGC 7 cut(s) 193, 251, 488, 591, 881, 1238, 1421
BseYI CCCAGC 1 cut(s) 8
BsgI GTGCAG 4 cut(s) 210, 693, 1450, 1496
Bsh1236I CGCG 1 cut(s) 277
BshFI GGCC 8 cut(s) 194, 304, 353, 1072, 1306, 1447, 1509, 1548
BshNI GGYRCC 1 cut(s) 1011
BshTI ACCGGT 1 cut(s) 1014
BsiHKCI CYCGRG 1 cut(s) 119
BsiSI CCGG 3 cut(s) 961, 1015, 1303
BslFI GGGAC 2 cut(s) 975, 1436
BslI CCNNNNNNNGG 3 cut(s) 62, 1252, 1372
BsmAI GTCTC 3 cut(s) 74, 193, 547
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 2 cut(s) 975, 1436
BsmI GAATGC 2 cut(s) 1142, 1497
BsnI GGCC 8 cut(s) 194, 304, 353, 1072, 1306, 1447, 1509, 1548
BsoBI CYCGRG 1 cut(s) 119
Bsp119I TTCGAA 1 cut(s) 661
Bsp143I GATC 3 cut(s) 359, 920, 1235
BspANI GGCC 8 cut(s) 194, 304, 353, 1072, 1306, 1447, 1509, 1548
BspCNI CTCAG 5 cut(s) 179, 189, 790, 1312, 1515
BspFNI CGCG 1 cut(s) 277
BspHI TCATGA 2 cut(s) 409, 1501
BspLI GGNNCC 6 cut(s) 6, 294, 726, 991, 1013, 1257
BspMAI CTGCAG 1 cut(s) 574
BspPI GGATC 2 cut(s) 928, 1243
BspT104I TTCGAA 1 cut(s) 661
BspT107I GGYRCC 1 cut(s) 1011
BspTI CTTAAG 1 cut(s) 88
BsrDI GCAATG 1 cut(s) 1331
BsrFI RCCGGY 1 cut(s) 1014
BsrI ACTGG 4 cut(s) 270, 890, 1479, 1520
BssAI RCCGGY 1 cut(s) 1014
BssECI CCNNGG 5 cut(s) 617, 1105, 1171, 1251, 1550
BssHII GCGCGC 1 cut(s) 275
BssMI GATC 3 cut(s) 359, 920, 1235
BssNI GRCGYC 1 cut(s) 479
BssSI CACGAG 1 cut(s) 1424
BssT1I CCWWGG 2 cut(s) 1171, 1550
Bst2BI CACGAG 1 cut(s) 1424
Bst2UI CCWGG 3 cut(s) 355, 1253, 1260
Bst4CI ACNGT 6 cut(s) 184, 625, 859, 950, 957, 1106
BstACI GRCGYC 1 cut(s) 479
BstAFI CTTAAG 1 cut(s) 88
BstAPI GCANNNNNTGC 1 cut(s) 1018
BstBAI YACGTR 1 cut(s) 731
BstBI TTCGAA 1 cut(s) 661
BstC8I GCNNGC 3 cut(s) 277, 1407, 1511
BstDEI CTNAG 5 cut(s) 166, 197, 798, 1320, 1523
BstDSI CCRYGG 2 cut(s) 617, 1105
BstEII GGTNACC 1 cut(s) 509
BstF5I GGATG 6 cut(s) 334, 430, 856, 872, 1246, 1263
BstFNI CGCG 1 cut(s) 277
BstHHI GCGC 4 cut(s) 255, 277, 279, 579
BstKTI GATC 3 cut(s) 362, 923, 1238
BstMAI GTCTC 3 cut(s) 74, 193, 547
BstMBI GATC 3 cut(s) 359, 920, 1235
BstMWI GCNNNNNNNGC 5 cut(s) 239, 283, 476, 1018, 1418
BstNI CCWGG 3 cut(s) 355, 1253, 1260
BstNSI RCATGY 2 cut(s) 1167, 1464
BstPI GGTNACC 1 cut(s) 509
BstSCI CCNGG 4 cut(s) 353, 960, 1251, 1258
BstSFI CTRYAG 1 cut(s) 570
BstUI CGCG 1 cut(s) 277
BstV1I GCAGC 7 cut(s) 193, 251, 488, 591, 881, 1238, 1421
BstV2I GAAGAC 1 cut(s) 798
BstX2I RGATCY 1 cut(s) 920
BstXI CCANNNNNNTGG 1 cut(s) 1178
BstYI RGATCY 1 cut(s) 920
BsuRI GGCC 8 cut(s) 194, 304, 353, 1072, 1306, 1447, 1509, 1548
BtgI CCRYGG 2 cut(s) 617, 1105
BtsCI GGATG 6 cut(s) 334, 430, 856, 872, 1246, 1263
BtsI GCAGTG 2 cut(s) 1028, 1455
BtsIMutI CAGTG 6 cut(s) 795, 955, 1028, 1455, 1486, 1513
Cac8I GCNNGC 3 cut(s) 277, 1407, 1511
CciI TCATGA 2 cut(s) 409, 1501
CfoI GCGC 4 cut(s) 255, 277, 279, 579
Cfr10I RCCGGY 1 cut(s) 1014
Cfr13I GGNCC 8 cut(s) 193, 302, 887, 989, 1070, 1243, 1446, 1547
CseI GACGC 2 cut(s) 468, 1156
Csp6I GTAC 5 cut(s) 958, 1057, 1209, 1340, 1346
CspAI ACCGGT 1 cut(s) 1014
CviQI GTAC 5 cut(s) 958, 1057, 1209, 1340, 1346
DdeI CTNAG 5 cut(s) 166, 197, 798, 1320, 1523
DpnI GATC 3 cut(s) 361, 922, 1237
DpnII GATC 3 cut(s) 359, 920, 1235
DraI TTTAAA 2 cut(s) 984, 1086
EaeI YGGCCR 1 cut(s) 351
Eco130I CCWWGG 2 cut(s) 1171, 1550
Eco32I GATATC 1 cut(s) 1113
Eco47I GGWCC 3 cut(s) 887, 989, 1243
Eco57I CTGAAG 4 cut(s) 9, 119, 255, 1086
Eco72I CACGTG 1 cut(s) 731
Eco88I CYCGRG 1 cut(s) 119
Eco91I GGTNACC 1 cut(s) 509
EcoO109I RGGNCCY 2 cut(s) 193, 989
EcoO65I GGTNACC 1 cut(s) 509
EcoRI GAATTC 1 cut(s) 663
EcoRII CCWGG 3 cut(s) 353, 1251, 1258
EcoRV GATATC 1 cut(s) 1113
EcoT14I CCWWGG 2 cut(s) 1171, 1550
EcoT22I ATGCAT 1 cut(s) 1372
ErhI CCWWGG 2 cut(s) 1171, 1550
Esp3I CGTCTC 1 cut(s) 193
FalI AAGNNNNNCTT 2 cut(s) 813, 845
FaqI GGGAC 2 cut(s) 975, 1436
Fnu4HI GCNGC 7 cut(s) 207, 240, 502, 580, 870, 1227, 1410
FokI GGATG 6 cut(s) 341, 437, 859, 863, 1233, 1250
Fsp4HI GCNGC 7 cut(s) 207, 240, 502, 580, 870, 1227, 1410
FspBI CTAG 1 cut(s) 1494
GlaI GCGC 4 cut(s) 254, 276, 278, 578
GluI GCNGC 7 cut(s) 207, 240, 502, 580, 870, 1227, 1410
GsaI CCCAGC 1 cut(s) 12
GsuI CTGGAG 2 cut(s) 287, 1251
HaeIII GGCC 8 cut(s) 194, 304, 353, 1072, 1306, 1447, 1509, 1548
HapII CCGG 3 cut(s) 961, 1015, 1303
HgaI GACGC 2 cut(s) 468, 1156
HhaI GCGC 4 cut(s) 255, 277, 279, 579
Hin1I GRCGYC 1 cut(s) 479
Hin6I GCGC 4 cut(s) 253, 275, 277, 577
HinP1I GCGC 4 cut(s) 253, 275, 277, 577
HincII GTYRAC 1 cut(s) 826
HindII GTYRAC 1 cut(s) 826
HinfI GANTC 8 cut(s) 443, 755, 937, 974, 1040, 1472, 1519, 1531
HpaII CCGG 3 cut(s) 961, 1015, 1303
HphI GGTGA 5 cut(s) 41, 440, 521, 605, 1427
Hpy166II GTNNAC 3 cut(s) 587, 826, 953
Hpy188I TCNGA 6 cut(s) 97, 169, 376, 686, 1311, 1524
Hpy188III TCNNGA 8 cut(s) 119, 137, 290, 410, 422, 778, 971, 1502
Hpy8I GTNNAC 3 cut(s) 587, 826, 953
HpyAV CCTTC 4 cut(s) 94, 206, 1061, 1384
HpyCH4III ACNGT 6 cut(s) 184, 625, 859, 950, 957, 1106
HpyCH4IV ACGT 1 cut(s) 730
HpyF10VI GCNNNNNNNGC 5 cut(s) 239, 283, 476, 1018, 1418
HpyF3I CTNAG 5 cut(s) 166, 197, 798, 1320, 1523
HpySE526I ACGT 1 cut(s) 730
Hsp92I GRCGYC 1 cut(s) 479
HspAI GCGC 4 cut(s) 253, 275, 277, 577
Kzo9I GATC 3 cut(s) 359, 920, 1235
LmnI GCTCC 3 cut(s) 10, 724, 813
Lsp1109I GCAGC 7 cut(s) 193, 251, 488, 591, 881, 1238, 1421
LweI GCATC 6 cut(s) 22, 295, 881, 1272, 1385, 1408
MaeI CTAG 1 cut(s) 1494
MaeII ACGT 1 cut(s) 730
MaeIII GTNAC 8 cut(s) 509, 518, 748, 835, 1093, 1145, 1159, 1327
MalI GATC 3 cut(s) 361, 922, 1237
MboI GATC 3 cut(s) 359, 920, 1235
MboII GAAGA 4 cut(s) 15, 120, 410, 803
MfeI CAATTG 1 cut(s) 1098
MflI RGATCY 1 cut(s) 920
MlsI TGGCCA 1 cut(s) 353
MluCI AATT 6 cut(s) 19, 73, 646, 663, 1098, 1129
MluNI TGGCCA 1 cut(s) 353
MlyI GAGTC 3 cut(s) 764, 931, 1525
Mox20I TGGCCA 1 cut(s) 353
Mph1103I ATGCAT 1 cut(s) 1372
MroXI GAANNNNTTC 2 cut(s) 220, 417
MscI TGGCCA 1 cut(s) 353
MseI TTAA 5 cut(s) 89, 983, 1085, 1353, 1490
MslI CAYNNNNRTG 2 cut(s) 531, 1426
Msp20I TGGCCA 1 cut(s) 353
MspA1I CMGCKG 3 cut(s) 242, 1229, 1412
MspCI CTTAAG 1 cut(s) 88
MspI CCGG 3 cut(s) 961, 1015, 1303
MspR9I CCNGG 4 cut(s) 355, 962, 1253, 1260
MunI CAATTG 1 cut(s) 1098
Mva1269I GAATGC 2 cut(s) 1142, 1497
MvaI CCWGG 3 cut(s) 355, 1253, 1260
MvnI CGCG 1 cut(s) 277
MwoI GCNNNNNNNGC 5 cut(s) 239, 283, 476, 1018, 1418
NciI CCSGG 1 cut(s) 962
NdeII GATC 3 cut(s) 359, 920, 1235
NlaIV GGNNCC 6 cut(s) 6, 294, 726, 991, 1013, 1257
NmuCI GTSAC 6 cut(s) 509, 518, 748, 1145, 1159, 1327
NsiI ATGCAT 1 cut(s) 1372
NspI RCATGY 2 cut(s) 1167, 1464
NspV TTCGAA 1 cut(s) 661
OliI CACNNNNGTG 1 cut(s) 1426
PaeR7I CTCGAG 1 cut(s) 119
PagI TCATGA 2 cut(s) 409, 1501
PauI GCGCGC 1 cut(s) 275
PctI GAATGC 2 cut(s) 1142, 1497
PdmI GAANNNNTTC 2 cut(s) 220, 417
PfeI GAWTC 5 cut(s) 443, 974, 1040, 1472, 1519
PflFI GACNNNGTC 2 cut(s) 539, 754
PflMI CCANNNNNTGG 1 cut(s) 62
PinAI ACCGGT 1 cut(s) 1014
PkrI GCNGC 7 cut(s) 208, 241, 503, 581, 871, 1228, 1411
PleI GAGTC 3 cut(s) 763, 931, 1525
PmaCI CACGTG 1 cut(s) 731
PmlI CACGTG 1 cut(s) 731
PpsI GAGTC 3 cut(s) 763, 931, 1525
Ppu21I YACGTR 1 cut(s) 731
PpuMI RGGWCCY 1 cut(s) 989
Psp5II RGGWCCY 1 cut(s) 989
Psp6I CCWGG 3 cut(s) 353, 1251, 1258
PspCI CACGTG 1 cut(s) 731
PspEI GGTNACC 1 cut(s) 509
PspFI CCCAGC 1 cut(s) 8
PspGI CCWGG 3 cut(s) 353, 1251, 1258
PspN4I GGNNCC 6 cut(s) 6, 294, 726, 991, 1013, 1257
PspPI GGNCC 8 cut(s) 193, 302, 887, 989, 1070, 1243, 1446, 1547
PspPPI RGGWCCY 1 cut(s) 989
PstI CTGCAG 1 cut(s) 574
PsuI RGATCY 1 cut(s) 920
PsyI GACNNNGTC 2 cut(s) 539, 754
PteI GCGCGC 1 cut(s) 275
PvuII CAGCTG 3 cut(s) 242, 1229, 1412
RsaI GTAC 5 cut(s) 959, 1058, 1210, 1341, 1347
RsaNI GTAC 5 cut(s) 958, 1057, 1209, 1340, 1346
RseI CAYNNNNRTG 2 cut(s) 531, 1426
SaqAI TTAA 5 cut(s) 89, 983, 1085, 1353, 1490
SatI GCNGC 7 cut(s) 207, 240, 502, 580, 870, 1227, 1410
Sau3AI GATC 3 cut(s) 359, 920, 1235
Sau96I GGNCC 8 cut(s) 193, 302, 887, 989, 1070, 1243, 1446, 1547
ScaI AGTACT 1 cut(s) 1210
SchI GAGTC 3 cut(s) 764, 931, 1525
ScrFI CCNGG 4 cut(s) 355, 962, 1253, 1260
SfaNI GCATC 6 cut(s) 22, 295, 881, 1272, 1385, 1408
SfcI CTRYAG 1 cut(s) 570
Sfr274I CTCGAG 1 cut(s) 119
SfuI TTCGAA 1 cut(s) 661
SinI GGWCC 3 cut(s) 887, 989, 1243
SlaI CTCGAG 1 cut(s) 119
SmiMI CAYNNNNRTG 2 cut(s) 531, 1426
SmlI CTYRAG 2 cut(s) 88, 119
SmoI CTYRAG 2 cut(s) 88, 119
Sse9I AATT 6 cut(s) 19, 73, 646, 663, 1098, 1129
SspMI CTAG 1 cut(s) 1494
StyD4I CCNGG 4 cut(s) 353, 960, 1251, 1258
StyI CCWWGG 2 cut(s) 1171, 1550
TaaI ACNGT 6 cut(s) 184, 625, 859, 950, 957, 1106
TaiI ACGT 1 cut(s) 733
TaqI TCGA 4 cut(s) 108, 120, 661, 1193
TasI AATT 6 cut(s) 19, 73, 646, 663, 1098, 1129
TatI WGTACW 2 cut(s) 1208, 1339
TfiI GAWTC 5 cut(s) 443, 974, 1040, 1472, 1519
Tru1I TTAA 5 cut(s) 89, 983, 1085, 1353, 1490
Tru9I TTAA 5 cut(s) 89, 983, 1085, 1353, 1490
TscAI CASTG 6 cut(s) 802, 955, 1028, 1462, 1486, 1520
TseFI GTSAC 6 cut(s) 509, 518, 748, 1145, 1159, 1327
TseI GCWGC 7 cut(s) 206, 239, 501, 579, 869, 1226, 1409
Tsp45I GTSAC 6 cut(s) 509, 518, 748, 1145, 1159, 1327
TspDTI ATGAA 6 cut(s) 33, 276, 426, 483, 1209, 1490
TspGWI ACGGA 1 cut(s) 634
TspRI CASTG 6 cut(s) 802, 955, 1028, 1462, 1486, 1520
Tth111I GACNNNGTC 2 cut(s) 539, 754
Van91I CCANNNNNTGG 1 cut(s) 62
Vha464I CTTAAG 1 cut(s) 88
VpaK11BI GGWCC 3 cut(s) 887, 989, 1243
XapI RAATTY 3 cut(s) 646, 663, 1129
XceI RCATGY 2 cut(s) 1167, 1464
XhoI CTCGAG 1 cut(s) 119
XmnI GAANNNNTTC 2 cut(s) 220, 417
XspI CTAG 1 cut(s) 1494
ZrmI AGTACT 1 cut(s) 1210
Zsp2I ATGCAT 1 cut(s) 1372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.