Rroxscaffold_1G00028620

NADH kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000832
Physical Location & Seq
Reverse (-)
36476118 .. 36487295
11178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00028620.1

Sequence Viewer

Length: 1635 bp
ATGGCTCCCAGCAAGTCCAATTCTTCAGGGGATGCAAGTGTTTCAGGCCCACAACCAGAGAATGGTTTTCTCAATTCACTTTCCCTCTTAAGTTCAGAAAAGGCAGTCGAGGAACTTCTCGAGCAATCTCCTCTTCAGGAAGCAGATGACCACCTCATAGAGTTCTCAGAGGCTATGAGAACGGTTGCAAAGGCCCTGAGACGAGCTGCTGAAGGAAAGGCTTCTGCACAGGCTGAAGCAGCTGAATGGAAGCGCAAATATGAACTGGAGAGGGCGCACAATCTGCATCTGGAACATGAAGTGCTATTATCACAACTGTTAGAAGCCTTGCTTAAGTACTTGAGTTCATGGGACTTTGTTTCCTTTAGTGTTGGGCCATCACATAGAGAAAATAACGGGGATGATGAGAGGATAGAGAACTTGGCCAGGAGATCAAATGACCAATCTGAACAATGTTGTGGGAGCAATGGGATATGCTCTCATGAAGTTCTTCGGGATGGTGAATGTGATACTGATTCAAAATCGCTTCTCAATAAGTTTGCAAGAAAGGCGTCATTCAAACTTTCATGGTGCTGCAAAGGTGACCAGAGTGACCAGCACAAGCATGACATTGTCTCTTTTGAAAGAGGAAACATAACAACTGCAGAGCGCAGCAGTAAACAGATTTCTTTGAAGTGGGAAACTCACCCACGGACTGTGCTTATATTGACCAAACCAAATTCAACTTCTGTTCGAATTCTGTGTGCAGAAATGGTCAGATGGTTGAGAGAGCAGAAAAAGTTAGATATTTATGTGGAGCCACGTGTGAGAGTTGAACTTGTGACAGAGTCATCGTACTACAACTTCGTCCAGACATGGAAAGAAGACACTGAGATTATGCTCCTGCATACAAAAGTTGACCTTGTGGTAACTCTTGGTGGGGATGGAACCGTCCTCTGGGCAGCATCCATGTTCAAAGGACCAGTTCCTCCTGTTGTGCCGTTTTCTTTAGGATCTCTGGGATTTATGACTCCATTTCACAGTGAACGGTACCAGGTTTATCTTGATTCACTTTTAAAAGGTCCCATTAGTATCACGCTACGGCACCGGTTGCAGTGCCATGTTATTAGAGAAGCAGCTAAAAATGAGTACGAAACTGAAGGGCCAATACTTGTTTTAAATGAGGTTACAATTGACCGTGGGATATCATCATATCTCACAAATTTGGAATGCTATTGTGACAACTCTTTTGTCACATGCGTCCAAGGAGATGGGCTGATATTATCGACAACATCTGGCAGTACTGCATATTCATTAGCAGCTGGAGGATCAATGGTCCATCCCCAGGTTCCAGGCATCCTCTTTACCCCTATTTGTCCTCATTCCCTATCATTCCGGCCTCTGATATTACCTGAGTATGTGACATTGCGAGTACAAGTACCTTTTAACAGCAGAAGCCATGCATGGGCGTCATTTGATGGGAAGGACAGGAAACGGCTGGCACCTGGTGATGCACTCGTGTGCAGTATGGCTACTTGGCCCGTCCCCACTGCATGTCAAGTTGATTCAACTAATGATTTCTTAACTAGCATTCATGATGGCCTGCACTGGAATCTGAGAAAGACTCAATCTTATGATGGCCCTCGGGATATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

544

Amino Acids

60.78

Weight (kDa)

5.87

Isoelectric Point (pI)

43.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_kinase PF01513 233 - 354 3.7e-18 ATP-NAD kinase N-terminal domain
NAD_kinase_C PF20143 386 - 501 2.1e-30 ATP-NAD kinase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G21070 AT3G21070 AT3G21070 AT3G21070
fragaria_vesca FvH4_3g28510
malus_domestica MD03G1172500.v1.1 MD11G1190400.v1.1
prunus_persica Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1 Prupe.4G254100_v2.0.a1
pyrus_communis pycom03g12970 pycom11g16670
rosa_chinensis RchiOBHm_Chr5g0052801
rosa_laevigata RLG00000034795
rosa_multiflora Rmu_sc0004335.1_g000006 Rmu_sc0032421.1_g000001 Rmu_ssc0000311.1_g000025
rosa_roxburghii Rroxscaffold_1G00028620 Rroxscaffold_4G00318540
rosa_rugosa Rorug05G0269700 Rorug05G0269800
rosa_samantha Rh5AG341600 Rh5BG352300 Rh5CG379000 Rh5DG368700
rosa_wichuraiana Rw5G032210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1029
AccB1I GGYRCC 3 cut(s) 1029, 1083, 1480
AccB7I CCANNNNNTGG 1 cut(s) 62
AclWI GGATC 2 cut(s) 1000, 1315
AcoI YGGCCR 1 cut(s) 423
AcsI RAATTY 3 cut(s) 718, 735, 1201
AcuI CTGAAG 5 cut(s) 9, 119, 231, 255, 1158
AcvI CACGTG 1 cut(s) 803
AcyI GRCGYC 2 cut(s) 551, 1448
AdeI CACNNNGTG 1 cut(s) 1487
AfaI GTAC 7 cut(s) 338, 836, 1031, 1130, 1282, 1413, 1419
AfiI CCNNNNNNNGG 4 cut(s) 62, 936, 1324, 1444
AflII CTTAAG 2 cut(s) 88, 332
AflIII ACRYGT 1 cut(s) 802
AgeI ACCGGT 1 cut(s) 1086
AgsI TTSAA 8 cut(s) 519, 559, 623, 673, 723, 815, 955, 1548
AjnI CCWGG 5 cut(s) 425, 1032, 1323, 1330, 1483
AjuI GAANNNNNNNTTGG 2 cut(s) 709, 741
AleI CACNNNNGTG 1 cut(s) 1498
AluBI AGCT 4 cut(s) 206, 242, 1118, 1301
AluI AGCT 4 cut(s) 206, 242, 1118, 1301
Alw26I GTCTC 2 cut(s) 193, 619
AlwI GGATC 2 cut(s) 1000, 1315
Ama87I CYCGRG 2 cut(s) 119, 1623
AoxI GGCC 9 cut(s) 46, 192, 374, 423, 1142, 1376, 1517, 1579, 1618
ApeKI GCWGC 7 cut(s) 206, 239, 573, 651, 941, 1115, 1298
ApoI RAATTY 3 cut(s) 718, 735, 1201
ArsI GACNNNNNNTTYG 2 cut(s) 1211, 1243
AsiGI ACCGGT 1 cut(s) 1086
Asp700I GAANNNNTTC 2 cut(s) 220, 489
Asp718I GGTACC 1 cut(s) 1029
AspLEI GCGC 3 cut(s) 255, 277, 651
AspS9I GGNCC 9 cut(s) 47, 193, 374, 959, 1061, 1142, 1315, 1518, 1619
AsuHPI GGTGA 4 cut(s) 512, 593, 677, 1499
AsuII TTCGAA 1 cut(s) 733
AvaI CYCGRG 2 cut(s) 119, 1623
AvaII GGWCC 3 cut(s) 959, 1061, 1315
BalI TGGCCA 1 cut(s) 425
BanI GGYRCC 3 cut(s) 1029, 1083, 1480
BarI GAAGNNNNNNTAC 4 cut(s) 827, 859, 1131, 1163
BauI CACGAG 1 cut(s) 1496
BbrPI CACGTG 1 cut(s) 803
BbsI GAAGAC 1 cut(s) 870
BbvI GCAGC 7 cut(s) 193, 251, 560, 663, 953, 1127, 1310
BccI CCATC 9 cut(s) 385, 491, 753, 917, 1244, 1326, 1451, 1571, 1610
BceAI ACGGC 3 cut(s) 964, 1097, 1490
BciT130I CCWGG 5 cut(s) 427, 1034, 1325, 1332, 1485
BcoDI GTCTC 2 cut(s) 193, 619
BfaI CTAG 1 cut(s) 1566
BfmI CTRYAG 1 cut(s) 642
BfrI CTTAAG 2 cut(s) 88, 332
BisI GCNGC 7 cut(s) 207, 240, 574, 652, 942, 1116, 1299
BlsI GCNGC 7 cut(s) 208, 241, 575, 653, 943, 1117, 1300
BmcAI AGTACT 2 cut(s) 338, 1282
Bme1390I CCNGG 5 cut(s) 427, 1034, 1325, 1332, 1485
Bme18I GGWCC 3 cut(s) 959, 1061, 1315
BmeT110I CYCGRG 2 cut(s) 119, 1623
BmgT120I GGNCC 9 cut(s) 47, 193, 374, 959, 1061, 1142, 1315, 1518, 1619
BmiI GGNNCC 8 cut(s) 6, 798, 928, 1031, 1063, 1085, 1329, 1482
BmrFI CCNGG 5 cut(s) 427, 1034, 1325, 1332, 1485
BmsI GCATC 5 cut(s) 22, 295, 953, 1344, 1480
BpiI GAAGAC 1 cut(s) 870
BplI GAGNNNNNCTC 2 cut(s) 1588, 1620
BpmI CTGGAG 2 cut(s) 287, 1323
Bpu14I TTCGAA 1 cut(s) 733
BpuEI CTTGAG 1 cut(s) 361
BsaAI YACGTR 1 cut(s) 803
BsaHI GRCGYC 2 cut(s) 551, 1448
BsaJI CCNNGG 5 cut(s) 689, 1177, 1243, 1323, 1622
BsaWI WCCGGW 1 cut(s) 1086
Bsc4I CCNNNNNNNGG 4 cut(s) 62, 936, 1324, 1444
Bse118I RCCGGY 1 cut(s) 1086
Bse1I ACTGG 3 cut(s) 270, 962, 1592
Bse3DI GCAATG 2 cut(s) 472, 1403
BseBI CCWGG 5 cut(s) 427, 1034, 1325, 1332, 1485
BseDI CCNNGG 5 cut(s) 689, 1177, 1243, 1323, 1622
BseGI GGATG 7 cut(s) 37, 406, 502, 928, 944, 1318, 1335
BseLI CCNNNNNNNGG 4 cut(s) 62, 936, 1324, 1444
BseMI GCAATG 2 cut(s) 472, 1403
BseMII CTCAG 5 cut(s) 180, 188, 861, 1383, 1586
BseNI ACTGG 3 cut(s) 270, 962, 1592
BseRI GAGGAG 1 cut(s) 120
BseXI GCAGC 7 cut(s) 193, 251, 560, 663, 953, 1127, 1310
BseYI CCCAGC 1 cut(s) 8
BsgI GTGCAG 4 cut(s) 210, 765, 1522, 1568
BshFI GGCC 9 cut(s) 48, 194, 376, 425, 1144, 1378, 1519, 1581, 1620
BshNI GGYRCC 3 cut(s) 1029, 1083, 1480
BshTI ACCGGT 1 cut(s) 1086
BsiHKCI CYCGRG 2 cut(s) 119, 1623
BsiSI CCGG 2 cut(s) 1087, 1375
BslFI GGGAC 3 cut(s) 365, 1047, 1508
BslI CCNNNNNNNGG 4 cut(s) 62, 936, 1324, 1444
BsmAI GTCTC 2 cut(s) 193, 619
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 3 cut(s) 365, 1047, 1508
BsmI GAATGC 2 cut(s) 1214, 1569
BsnI GGCC 9 cut(s) 48, 194, 376, 425, 1144, 1378, 1519, 1581, 1620
BsoBI CYCGRG 2 cut(s) 119, 1623
Bsp119I TTCGAA 1 cut(s) 733
Bsp143I GATC 3 cut(s) 431, 992, 1307
BspANI GGCC 9 cut(s) 48, 194, 376, 425, 1144, 1378, 1519, 1581, 1620
BspCNI CTCAG 5 cut(s) 179, 189, 862, 1384, 1587
BspHI TCATGA 2 cut(s) 481, 1573
BspLI GGNNCC 8 cut(s) 6, 798, 928, 1031, 1063, 1085, 1329, 1482
BspMAI CTGCAG 1 cut(s) 646
BspPI GGATC 2 cut(s) 1000, 1315
BspT104I TTCGAA 1 cut(s) 733
BspT107I GGYRCC 3 cut(s) 1029, 1083, 1480
BspTI CTTAAG 2 cut(s) 88, 332
BsrDI GCAATG 2 cut(s) 472, 1403
BsrFI RCCGGY 1 cut(s) 1086
BsrI ACTGG 3 cut(s) 270, 962, 1592
BssAI RCCGGY 1 cut(s) 1086
BssECI CCNNGG 5 cut(s) 689, 1177, 1243, 1323, 1622
BssMI GATC 3 cut(s) 431, 992, 1307
BssNI GRCGYC 2 cut(s) 551, 1448
BssSI CACGAG 1 cut(s) 1496
BssT1I CCWWGG 1 cut(s) 1243
Bst2BI CACGAG 1 cut(s) 1496
Bst2UI CCWGG 5 cut(s) 427, 1034, 1325, 1332, 1485
Bst4CI ACNGT 7 cut(s) 184, 318, 697, 931, 1022, 1029, 1178
Bst6I CTCTTC 1 cut(s) 138
BstACI GRCGYC 2 cut(s) 551, 1448
BstAFI CTTAAG 2 cut(s) 88, 332
BstAPI GCANNNNNTGC 2 cut(s) 283, 1090
BstBAI YACGTR 1 cut(s) 803
BstBI TTCGAA 1 cut(s) 733
BstC8I GCNNGC 2 cut(s) 1479, 1583
BstDEI CTNAG 5 cut(s) 166, 197, 870, 1392, 1595
BstDSI CCRYGG 2 cut(s) 689, 1177
BstEII GGTNACC 1 cut(s) 581
BstF5I GGATG 7 cut(s) 37, 406, 502, 928, 944, 1318, 1335
BstHHI GCGC 3 cut(s) 255, 277, 651
BstKTI GATC 3 cut(s) 434, 995, 1310
BstMAI GTCTC 2 cut(s) 193, 619
BstMBI GATC 3 cut(s) 431, 992, 1307
BstMWI GCNNNNNNNGC 4 cut(s) 239, 283, 548, 1090
BstNI CCWGG 5 cut(s) 427, 1034, 1325, 1332, 1485
BstNSI RCATGY 2 cut(s) 1239, 1536
BstPI GGTNACC 1 cut(s) 581
BstSCI CCNGG 5 cut(s) 425, 1032, 1323, 1330, 1483
BstSFI CTRYAG 1 cut(s) 642
BstV1I GCAGC 7 cut(s) 193, 251, 560, 663, 953, 1127, 1310
BstV2I GAAGAC 1 cut(s) 870
BstX2I RGATCY 1 cut(s) 992
BstXI CCANNNNNNTGG 1 cut(s) 1250
BstYI RGATCY 1 cut(s) 992
BsuRI GGCC 9 cut(s) 48, 194, 376, 425, 1144, 1378, 1519, 1581, 1620
BtgI CCRYGG 2 cut(s) 689, 1177
BtsCI GGATG 7 cut(s) 37, 406, 502, 928, 944, 1318, 1335
BtsI GCAGTG 2 cut(s) 1100, 1527
BtsIMutI CAGTG 5 cut(s) 867, 1027, 1100, 1527, 1585
Cac8I GCNNGC 2 cut(s) 1479, 1583
CciI TCATGA 2 cut(s) 481, 1573
CfoI GCGC 3 cut(s) 255, 277, 651
Cfr10I RCCGGY 1 cut(s) 1086
Cfr13I GGNCC 9 cut(s) 47, 193, 374, 959, 1061, 1142, 1315, 1518, 1619
CseI GACGC 3 cut(s) 540, 1228, 1437
CsiI ACCWGGT 2 cut(s) 1032, 1483
Csp6I GTAC 7 cut(s) 337, 835, 1030, 1129, 1281, 1412, 1418
CspAI ACCGGT 1 cut(s) 1086
CviQI GTAC 7 cut(s) 337, 835, 1030, 1129, 1281, 1412, 1418
DdeI CTNAG 5 cut(s) 166, 197, 870, 1392, 1595
DpnI GATC 3 cut(s) 433, 994, 1309
DpnII GATC 3 cut(s) 431, 992, 1307
DraI TTTAAA 2 cut(s) 1056, 1158
DraIII CACNNNGTG 1 cut(s) 1487
EaeI YGGCCR 1 cut(s) 423
Eam1104I CTCTTC 1 cut(s) 138
EarI CTCTTC 1 cut(s) 138
Eco130I CCWWGG 1 cut(s) 1243
Eco32I GATATC 1 cut(s) 1185
Eco47I GGWCC 3 cut(s) 959, 1061, 1315
Eco57I CTGAAG 5 cut(s) 9, 119, 231, 255, 1158
Eco72I CACGTG 1 cut(s) 803
Eco88I CYCGRG 2 cut(s) 119, 1623
Eco91I GGTNACC 1 cut(s) 581
EcoO109I RGGNCCY 2 cut(s) 193, 1061
EcoO65I GGTNACC 1 cut(s) 581
EcoRI GAATTC 1 cut(s) 735
EcoRII CCWGG 5 cut(s) 425, 1032, 1323, 1330, 1483
EcoRV GATATC 1 cut(s) 1185
EcoT14I CCWWGG 1 cut(s) 1243
EcoT22I ATGCAT 1 cut(s) 1444
ErhI CCWWGG 1 cut(s) 1243
Esp3I CGTCTC 1 cut(s) 193
FalI AAGNNNNNCTT 4 cut(s) 315, 347, 885, 917
FaqI GGGAC 3 cut(s) 365, 1047, 1508
Fnu4HI GCNGC 7 cut(s) 207, 240, 574, 652, 942, 1116, 1299
FokI GGATG 7 cut(s) 44, 413, 509, 931, 935, 1305, 1322
Fsp4HI GCNGC 7 cut(s) 207, 240, 574, 652, 942, 1116, 1299
FspBI CTAG 1 cut(s) 1566
GlaI GCGC 3 cut(s) 254, 276, 650
GluI GCNGC 7 cut(s) 207, 240, 574, 652, 942, 1116, 1299
GsaI CCCAGC 1 cut(s) 12
GsuI CTGGAG 2 cut(s) 287, 1323
HaeIII GGCC 9 cut(s) 48, 194, 376, 425, 1144, 1378, 1519, 1581, 1620
HapII CCGG 2 cut(s) 1087, 1375
HgaI GACGC 3 cut(s) 540, 1228, 1437
HhaI GCGC 3 cut(s) 255, 277, 651
Hin1I GRCGYC 2 cut(s) 551, 1448
Hin6I GCGC 3 cut(s) 253, 275, 649
HinP1I GCGC 3 cut(s) 253, 275, 649
HincII GTYRAC 1 cut(s) 898
HindII GTYRAC 1 cut(s) 898
HinfI GANTC 7 cut(s) 515, 827, 1009, 1046, 1544, 1591, 1603
HpaII CCGG 2 cut(s) 1087, 1375
HphI GGTGA 4 cut(s) 512, 593, 677, 1499
Hpy166II GTNNAC 3 cut(s) 659, 898, 1025
Hpy188I TCNGA 6 cut(s) 97, 169, 448, 758, 1383, 1596
Hpy188III TCNNGA 9 cut(s) 119, 137, 290, 482, 494, 850, 1043, 1574, 1625
Hpy8I GTNNAC 3 cut(s) 659, 898, 1025
HpyAV CCTTC 3 cut(s) 206, 1133, 1456
HpyCH4III ACNGT 7 cut(s) 184, 318, 697, 931, 1022, 1029, 1178
HpyCH4IV ACGT 1 cut(s) 802
HpyF10VI GCNNNNNNNGC 4 cut(s) 239, 283, 548, 1090
HpyF3I CTNAG 5 cut(s) 166, 197, 870, 1392, 1595
HpySE526I ACGT 1 cut(s) 802
Hsp92I GRCGYC 2 cut(s) 551, 1448
HspAI GCGC 3 cut(s) 253, 275, 649
KpnI GGTACC 1 cut(s) 1033
Kzo9I GATC 3 cut(s) 431, 992, 1307
LmnI GCTCC 4 cut(s) 10, 462, 796, 885
Lsp1109I GCAGC 7 cut(s) 193, 251, 560, 663, 953, 1127, 1310
LweI GCATC 5 cut(s) 22, 295, 953, 1344, 1480
MabI ACCWGGT 2 cut(s) 1032, 1483
MaeI CTAG 1 cut(s) 1566
MaeII ACGT 1 cut(s) 802
MaeIII GTNAC 8 cut(s) 581, 590, 820, 907, 1165, 1217, 1231, 1399
MalI GATC 3 cut(s) 433, 994, 1309
MboI GATC 3 cut(s) 431, 992, 1307
MboII GAAGA 4 cut(s) 15, 125, 482, 875
MfeI CAATTG 1 cut(s) 1170
MflI RGATCY 1 cut(s) 992
MlsI TGGCCA 1 cut(s) 425
MluCI AATT 6 cut(s) 19, 73, 718, 735, 1170, 1201
MluNI TGGCCA 1 cut(s) 425
MlyI GAGTC 3 cut(s) 836, 1003, 1597
Mox20I TGGCCA 1 cut(s) 425
Mph1103I ATGCAT 1 cut(s) 1444
MroXI GAANNNNTTC 2 cut(s) 220, 489
MscI TGGCCA 1 cut(s) 425
MseI TTAA 6 cut(s) 89, 333, 1055, 1157, 1425, 1562
MslI CAYNNNNRTG 2 cut(s) 603, 1498
Msp20I TGGCCA 1 cut(s) 425
MspA1I CMGCKG 2 cut(s) 242, 1301
MspCI CTTAAG 2 cut(s) 88, 332
MspI CCGG 2 cut(s) 1087, 1375
MspR9I CCNGG 5 cut(s) 427, 1034, 1325, 1332, 1485
MunI CAATTG 1 cut(s) 1170
Mva1269I GAATGC 2 cut(s) 1214, 1569
MvaI CCWGG 5 cut(s) 427, 1034, 1325, 1332, 1485
MwoI GCNNNNNNNGC 4 cut(s) 239, 283, 548, 1090
NdeII GATC 3 cut(s) 431, 992, 1307
NlaIV GGNNCC 8 cut(s) 6, 798, 928, 1031, 1063, 1085, 1329, 1482
NmuCI GTSAC 6 cut(s) 581, 590, 820, 1217, 1231, 1399
NsiI ATGCAT 1 cut(s) 1444
NspI RCATGY 2 cut(s) 1239, 1536
NspV TTCGAA 1 cut(s) 733
OliI CACNNNNGTG 1 cut(s) 1498
PaeR7I CTCGAG 1 cut(s) 119
PagI TCATGA 2 cut(s) 481, 1573
PctI GAATGC 2 cut(s) 1214, 1569
PdmI GAANNNNTTC 2 cut(s) 220, 489
PfeI GAWTC 4 cut(s) 515, 1046, 1544, 1591
PflFI GACNNNGTC 2 cut(s) 611, 826
PflMI CCANNNNNTGG 1 cut(s) 62
PinAI ACCGGT 1 cut(s) 1086
PkrI GCNGC 7 cut(s) 208, 241, 575, 653, 943, 1117, 1300
PleI GAGTC 3 cut(s) 835, 1003, 1597
PmaCI CACGTG 1 cut(s) 803
PmlI CACGTG 1 cut(s) 803
PpsI GAGTC 3 cut(s) 835, 1003, 1597
Ppu21I YACGTR 1 cut(s) 803
PpuMI RGGWCCY 1 cut(s) 1061
Psp5II RGGWCCY 1 cut(s) 1061
Psp6I CCWGG 5 cut(s) 425, 1032, 1323, 1330, 1483
PspCI CACGTG 1 cut(s) 803
PspEI GGTNACC 1 cut(s) 581
PspFI CCCAGC 1 cut(s) 8
PspGI CCWGG 5 cut(s) 425, 1032, 1323, 1330, 1483
PspN4I GGNNCC 8 cut(s) 6, 798, 928, 1031, 1063, 1085, 1329, 1482
PspPI GGNCC 9 cut(s) 47, 193, 374, 959, 1061, 1142, 1315, 1518, 1619
PspPPI RGGWCCY 1 cut(s) 1061
PstI CTGCAG 1 cut(s) 646
PsuI RGATCY 1 cut(s) 992
PsyI GACNNNGTC 2 cut(s) 611, 826
PvuII CAGCTG 2 cut(s) 242, 1301
RsaI GTAC 7 cut(s) 338, 836, 1031, 1130, 1282, 1413, 1419
RsaNI GTAC 7 cut(s) 337, 835, 1030, 1129, 1281, 1412, 1418
RseI CAYNNNNRTG 2 cut(s) 603, 1498
SaqAI TTAA 6 cut(s) 89, 333, 1055, 1157, 1425, 1562
SatI GCNGC 7 cut(s) 207, 240, 574, 652, 942, 1116, 1299
Sau3AI GATC 3 cut(s) 431, 992, 1307
Sau96I GGNCC 9 cut(s) 47, 193, 374, 959, 1061, 1142, 1315, 1518, 1619
ScaI AGTACT 2 cut(s) 338, 1282
SchI GAGTC 3 cut(s) 836, 1003, 1597
ScrFI CCNGG 5 cut(s) 427, 1034, 1325, 1332, 1485
SexAI ACCWGGT 2 cut(s) 1032, 1483
SfaNI GCATC 5 cut(s) 22, 295, 953, 1344, 1480
SfcI CTRYAG 1 cut(s) 642
Sfr274I CTCGAG 1 cut(s) 119
SfuI TTCGAA 1 cut(s) 733
SinI GGWCC 3 cut(s) 959, 1061, 1315
SlaI CTCGAG 1 cut(s) 119
SmiMI CAYNNNNRTG 2 cut(s) 603, 1498
SmlI CTYRAG 4 cut(s) 88, 119, 332, 340
SmoI CTYRAG 4 cut(s) 88, 119, 332, 340
Sse9I AATT 6 cut(s) 19, 73, 718, 735, 1170, 1201
SspMI CTAG 1 cut(s) 1566
StyD4I CCNGG 5 cut(s) 425, 1032, 1323, 1330, 1483
StyI CCWWGG 1 cut(s) 1243
TaaI ACNGT 7 cut(s) 184, 318, 697, 931, 1022, 1029, 1178
TaiI ACGT 1 cut(s) 805
TaqI TCGA 4 cut(s) 108, 120, 733, 1265
TasI AATT 6 cut(s) 19, 73, 718, 735, 1170, 1201
TatI WGTACW 3 cut(s) 336, 1280, 1411
TfiI GAWTC 4 cut(s) 515, 1046, 1544, 1591
Tru1I TTAA 6 cut(s) 89, 333, 1055, 1157, 1425, 1562
Tru9I TTAA 6 cut(s) 89, 333, 1055, 1157, 1425, 1562
TscAI CASTG 5 cut(s) 874, 1027, 1100, 1534, 1592
TseFI GTSAC 6 cut(s) 581, 590, 820, 1217, 1231, 1399
TseI GCWGC 7 cut(s) 206, 239, 573, 651, 941, 1115, 1298
Tsp45I GTSAC 6 cut(s) 581, 590, 820, 1217, 1231, 1399
TspDTI ATGAA 7 cut(s) 276, 312, 336, 498, 555, 1281, 1562
TspGWI ACGGA 1 cut(s) 706
TspRI CASTG 5 cut(s) 874, 1027, 1100, 1534, 1592
Tth111I GACNNNGTC 2 cut(s) 611, 826
Van91I CCANNNNNTGG 1 cut(s) 62
Vha464I CTTAAG 2 cut(s) 88, 332
VpaK11BI GGWCC 3 cut(s) 959, 1061, 1315
XapI RAATTY 3 cut(s) 718, 735, 1201
XceI RCATGY 2 cut(s) 1239, 1536
XhoI CTCGAG 1 cut(s) 119
XmnI GAANNNNTTC 2 cut(s) 220, 489
XspI CTAG 1 cut(s) 1566
ZrmI AGTACT 2 cut(s) 338, 1282
Zsp2I ATGCAT 1 cut(s) 1444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.