Prupe.7G000100_v2.0.a1
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0006438
Physical Location & Seq
Forward (+)
37514 .. 39612
2099 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G000100.1

Sequence Viewer

Length: 1359 bp
ATGAATTTCATGGATGAGGACGCTTCTTCGGGCTCTGGAGATGATGCAAACATGCTAGATGGAAACAAGCATCATTCTGGGGTACCATCAAGTTCCGGTCGTCGGAAGAGAAGTCGCAAGGCTACTGGCGATGCTATAGTGGATGCTATGCTTGAAATAGCTGCAGCTTCAAAAATGAGGGCAGCGGCAATCATGAAGAGCGAGGAAGGGTTTTCCATAAGCAAATGCATAAAGGTACTGGATGATATGCCAGATACAACAGCTGGCATGGATGATTTTGACTTAGAATTGGATGAGATGGAACTAACTGCGGCAGCGGCTGGCTACTACTATTATAACAGTATTACCAAGCAAACTTGTCGTAGTCTGTCACCTAGTAAAGAAAGTGGATTTATGACCGAAGTTCTCAATGGTCATGATGATGTGTTCCAGGAAATGTTTCGGATGGATAAAAATGTTTTTCACAAGTTATGTGACATTCTTCGGCAGAGAGGCATGTTACGTGATACTGCTGGTGTTATGATAGAGGAGCAGCTGGGAATTTTTTTGAATATTATTGGCCATAATGAACGTAACAGAGTAATCCAAGAGCGGTTTCATCATTCAGGTGAAACCATCAGCCGTCATTTCAATAATGTGTTGAAGGCAGTCAAGTCACTCTCTCGTGAATTCTTACAAACACCAACCCCCACCACCCCTCCAAACATCCTTGGGAATATTAGATTTTATCCATATTTCCAGGATTGTGTAGGAGTCATTGGTGGAATGCACATTCCAGCCCATGTCCCAGCCAAAGATCAATCCCGTTTTCGAAACAAGAAAGGTTCACGTGTATTGAAGGCAGTCCTTGATGATCCACATCAGAATTTTCCTTGTATTCCTGAAGGGAAATATTACCTTGTTGACTCAGGATATGCAAATATGAAAGGATTTATTGCTCCATTTCAAGGGGTAAGGTATCATGTTCATGGTTATAGAGGTGCTAATCAGTTACCTAGAAATGCAAGGGAGCTATTTAATCACAGGCACTCATCTCTTAGCAATGTCATCCAGAGGTCTTTTTCTTTGTTGAAGACTAGGTTTCCTATTCTCAAACTTGCTCCTCAGTATGCTGTTCATATTCAAAGAGATATAGTTATAGCTGCATGTGTTCTACACAATTACATCCGGCGTATGTCAGAAAAGGATTGGCTGTTTTTCGATACAGAAAAGATAACAATGGATGAATTGCCTGATCTTGATGAAAATCCTGACATGGAATTGGTTGCTTCGTTACAAGAACATGTTGCTTTTTCATTGAGAGAGTCAATTGCAGCAGAAATGTGGAATGACTTTATAAATAGATGGGATCAGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

453

Amino Acids

51.48

Weight (kDa)

6.42

Isoelectric Point (pI)

51.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 336, 1337
Acc65I GGTACC 1 cut(s) 82
AccB1I GGYRCC 1 cut(s) 82
AccBSI CCGCTC 1 cut(s) 592
AciI CCGC 4 cut(s) 185, 311, 317, 592
AclWI GGATC 2 cut(s) 848, 1356
AcoI YGGCCR 1 cut(s) 559
AcsI RAATTY 4 cut(s) 4, 540, 668, 865
AcuI CTGAAG 1 cut(s) 903
AcvI CACGTG 1 cut(s) 830
AfaI GTAC 2 cut(s) 84, 237
AfiI CCNNNNNNNGG 2 cut(s) 102, 947
AflIII ACRYGT 2 cut(s) 829, 1282
AgsI TTSAA 9 cut(s) 155, 171, 550, 631, 643, 838, 947, 1072, 1124
AjnI CCWGG 2 cut(s) 429, 738
AjuI GAANNNNNNNTTGG 2 cut(s) 579, 611
AluBI AGCT 6 cut(s) 161, 167, 263, 535, 1012, 1142
AluI AGCT 6 cut(s) 161, 167, 263, 535, 1012, 1142
AlwI GGATC 2 cut(s) 848, 1356
AlwNI CAGNNNCTG 1 cut(s) 320
AoxI GGCC 1 cut(s) 559
ApeKI GCWGC 7 cut(s) 161, 164, 182, 314, 532, 1142, 1313
ApoI RAATTY 4 cut(s) 4, 540, 668, 865
Asp700I GAANNNNTTC 1 cut(s) 438
Asp718I GGTACC 1 cut(s) 82
AsuHPI GGTGA 2 cut(s) 363, 620
AsuII TTCGAA 1 cut(s) 811
BalI TGGCCA 1 cut(s) 561
BanI GGYRCC 1 cut(s) 82
BanII GRGCYC 1 cut(s) 35
BauI CACGAG 1 cut(s) 663
BbrPI CACGTG 1 cut(s) 830
BbsI GAAGAC 1 cut(s) 1079
BbvI GCAGC 7 cut(s) 148, 176, 194, 326, 544, 1129, 1325
BccI CCATC 6 cut(s) 53, 94, 292, 439, 623, 1338
BceAI ACGGC 1 cut(s) 606
BcgI CGANNNNNNTGC 2 cut(s) 341, 375
BciT130I CCWGG 2 cut(s) 431, 740
BfaI CTAG 4 cut(s) 56, 375, 996, 1077
BfmI CTRYAG 2 cut(s) 135, 162
Bme1390I CCNGG 2 cut(s) 431, 740
BmiI GGNNCC 1 cut(s) 84
BmrFI CCNGG 2 cut(s) 431, 740
BmsI GCATC 4 cut(s) 34, 79, 121, 133
BpiI GAAGAC 1 cut(s) 1079
BpmI CTGGAG 1 cut(s) 57
Bpu14I TTCGAA 1 cut(s) 811
BsaAI YACGTR 2 cut(s) 503, 830
BsaBI GATNNNNATC 2 cut(s) 858, 1245
BsaJI CCNNGG 1 cut(s) 709
BsaWI WCCGGW 1 cut(s) 95
BsaXI ACNNNNNCTCC 2 cut(s) 682, 712
Bsc4I CCNNNNNNNGG 2 cut(s) 102, 947
Bse1I ACTGG 2 cut(s) 130, 243
Bse3DI GCAATG 1 cut(s) 1048
Bse8I GATNNNNATC 2 cut(s) 858, 1245
BseBI CCWGG 2 cut(s) 431, 740
BseDI CCNNGG 1 cut(s) 709
BseJI GATNNNNATC 2 cut(s) 858, 1245
BseLI CCNNNNNNNGG 2 cut(s) 102, 947
BseMI GCAATG 1 cut(s) 1048
BseMII CTCAG 2 cut(s) 921, 1118
BseNI ACTGG 2 cut(s) 130, 243
BseRI GAGGAG 2 cut(s) 542, 1092
BseXI GCAGC 7 cut(s) 148, 176, 194, 326, 544, 1129, 1325
BseYI CCCAGC 2 cut(s) 535, 787
Bsh1285I CGRYCG 1 cut(s) 100
BshFI GGCC 1 cut(s) 561
BshNI GGYRCC 1 cut(s) 82
BsiEI CGRYCG 1 cut(s) 100
BsiSI CCGG 2 cut(s) 96, 1168
BslFI GGGAC 1 cut(s) 770
BslI CCNNNNNNNGG 2 cut(s) 102, 947
BsmFI GGGAC 1 cut(s) 770
BsmI GAATGC 1 cut(s) 771
BsnI GGCC 1 cut(s) 561
Bsp119I TTCGAA 1 cut(s) 811
Bsp1286I GDGCHC 1 cut(s) 35
Bsp143I GATC 4 cut(s) 796, 853, 1234, 1348
BspACI CCGC 4 cut(s) 185, 311, 317, 592
BspANI GGCC 1 cut(s) 561
BspCNI CTCAG 2 cut(s) 920, 1117
BspHI TCATGA 2 cut(s) 192, 415
BspLI GGNNCC 1 cut(s) 84
BspMAI CTGCAG 1 cut(s) 166
BspPI GGATC 2 cut(s) 848, 1356
BspQI GCTCTTC 1 cut(s) 191
BspT104I TTCGAA 1 cut(s) 811
BspT107I GGYRCC 1 cut(s) 82
BsrBI CCGCTC 1 cut(s) 592
BsrDI GCAATG 1 cut(s) 1048
BsrI ACTGG 2 cut(s) 130, 243
BssECI CCNNGG 1 cut(s) 709
BssMI GATC 4 cut(s) 796, 853, 1234, 1348
BssSI CACGAG 1 cut(s) 663
BssT1I CCWWGG 1 cut(s) 709
Bst2BI CACGAG 1 cut(s) 663
Bst2UI CCWGG 2 cut(s) 431, 740
Bst4CI ACNGT 1 cut(s) 341
Bst6I CTCTTC 2 cut(s) 101, 191
BstBAI YACGTR 2 cut(s) 503, 830
BstBI TTCGAA 1 cut(s) 811
BstC8I GCNNGC 2 cut(s) 265, 322
BstDEI CTNAG 4 cut(s) 283, 907, 1037, 1104
BstKTI GATC 4 cut(s) 799, 856, 1237, 1351
BstMBI GATC 4 cut(s) 796, 853, 1234, 1348
BstMCI CGRYCG 1 cut(s) 100
BstMWI GCNNNNNNNGC 1 cut(s) 317
BstNI CCWGG 2 cut(s) 431, 740
BstNSI RCATGY 4 cut(s) 55, 499, 1149, 1286
BstSCI CCNGG 2 cut(s) 429, 738
BstSFI CTRYAG 2 cut(s) 135, 162
BstV1I GCAGC 7 cut(s) 148, 176, 194, 326, 544, 1129, 1325
BstV2I GAAGAC 1 cut(s) 1079
BsuRI GGCC 1 cut(s) 561
BtgZI GCGATG 1 cut(s) 144
BtsIMutI CAGTG 1 cut(s) 1358
Cac8I GCNNGC 2 cut(s) 265, 322
CaiI CAGNNNCTG 1 cut(s) 320
CciI TCATGA 2 cut(s) 192, 415
CseI GACGC 1 cut(s) 29
Csp6I GTAC 2 cut(s) 83, 236
CviQI GTAC 2 cut(s) 83, 236
DdeI CTNAG 4 cut(s) 283, 907, 1037, 1104
DpnI GATC 4 cut(s) 798, 855, 1236, 1350
DpnII GATC 4 cut(s) 796, 853, 1234, 1348
EaeI YGGCCR 1 cut(s) 559
Eam1104I CTCTTC 2 cut(s) 101, 191
EarI CTCTTC 2 cut(s) 101, 191
Eco130I CCWWGG 1 cut(s) 709
Eco24I GRGCYC 1 cut(s) 35
Eco57I CTGAAG 1 cut(s) 903
Eco72I CACGTG 1 cut(s) 830
EcoRI GAATTC 1 cut(s) 668
EcoRII CCWGG 2 cut(s) 429, 738
EcoT14I CCWWGG 1 cut(s) 709
EcoT22I ATGCAT 1 cut(s) 230
EcoT38I GRGCYC 1 cut(s) 35
ErhI CCWWGG 1 cut(s) 709
FaqI GGGAC 1 cut(s) 770
FriOI GRGCYC 1 cut(s) 35
FspBI CTAG 4 cut(s) 56, 375, 996, 1077
GsaI CCCAGC 2 cut(s) 539, 791
GsuI CTGGAG 1 cut(s) 57
HaeIII GGCC 1 cut(s) 561
HapII CCGG 2 cut(s) 96, 1168
HgaI GACGC 1 cut(s) 29
HincII GTYRAC 1 cut(s) 904
HindII GTYRAC 1 cut(s) 904
HinfI GANTC 3 cut(s) 753, 905, 1304
HpaII CCGG 2 cut(s) 96, 1168
HphI GGTGA 2 cut(s) 363, 620
Hpy166II GTNNAC 2 cut(s) 827, 904
Hpy188I TCNGA 4 cut(s) 105, 444, 864, 1180
Hpy188III TCNNGA 9 cut(s) 36, 193, 416, 665, 881, 909, 1051, 1238, 1250
Hpy8I GTNNAC 2 cut(s) 827, 904
Hpy99I CGWCG 1 cut(s) 105
HpyAV CCTTC 4 cut(s) 200, 637, 832, 878
HpyCH4III ACNGT 1 cut(s) 341
HpyCH4IV ACGT 3 cut(s) 502, 571, 829
HpyCH4V TGCA 8 cut(s) 47, 164, 228, 769, 917, 1004, 1145, 1313
HpyF10VI GCNNNNNNNGC 1 cut(s) 317
HpyF3I CTNAG 4 cut(s) 283, 907, 1037, 1104
HpySE526I ACGT 3 cut(s) 502, 571, 829
KpnI GGTACC 1 cut(s) 86
Kzo9I GATC 4 cut(s) 796, 853, 1234, 1348
LguI GCTCTTC 1 cut(s) 191
LmnI GCTCC 4 cut(s) 529, 943, 1009, 1105
Lsp1109I GCAGC 7 cut(s) 148, 176, 194, 326, 544, 1129, 1325
LweI GCATC 4 cut(s) 34, 79, 121, 133
MaeI CTAG 4 cut(s) 56, 375, 996, 1077
MaeII ACGT 3 cut(s) 502, 571, 829
MaeIII GTNAC 7 cut(s) 369, 473, 498, 572, 654, 990, 1272
MalI GATC 4 cut(s) 798, 855, 1236, 1350
MbiI CCGCTC 1 cut(s) 592
MboI GATC 4 cut(s) 796, 853, 1234, 1348
MboII GAAGA 5 cut(s) 18, 118, 208, 473, 1084
MfeI CAATTG 1 cut(s) 1308
MhlI GDGCHC 1 cut(s) 35
MlsI TGGCCA 1 cut(s) 561
MluCI AATT 9 cut(s) 4, 287, 540, 668, 865, 1159, 1226, 1259, 1308
MluNI TGGCCA 1 cut(s) 561
MlyI GAGTC 3 cut(s) 762, 899, 1313
MmeI TCCRAC 1 cut(s) 83
MnlI CCTC 9 cut(s) 10, 171, 196, 485, 520, 708, 971, 1047, 1113
Mox20I TGGCCA 1 cut(s) 561
Mph1103I ATGCAT 1 cut(s) 230
MroXI GAANNNNTTC 1 cut(s) 438
MscI TGGCCA 1 cut(s) 561
MseI TTAA 1 cut(s) 1017
MslI CAYNNNNRTG 3 cut(s) 420, 606, 966
Msp20I TGGCCA 1 cut(s) 561
MspA1I CMGCKG 4 cut(s) 185, 263, 317, 535
MspI CCGG 2 cut(s) 96, 1168
MspR9I CCNGG 2 cut(s) 431, 740
MunI CAATTG 1 cut(s) 1308
Mva1269I GAATGC 1 cut(s) 771
MvaI CCWGG 2 cut(s) 431, 740
MwoI GCNNNNNNNGC 1 cut(s) 317
NdeII GATC 4 cut(s) 796, 853, 1234, 1348
NlaIV GGNNCC 1 cut(s) 84
NmuCI GTSAC 3 cut(s) 369, 473, 654
NsiI ATGCAT 1 cut(s) 230
NspI RCATGY 4 cut(s) 55, 499, 1149, 1286
NspV TTCGAA 1 cut(s) 811
PagI TCATGA 2 cut(s) 192, 415
PciI ACATGT 1 cut(s) 1282
PciSI GCTCTTC 1 cut(s) 191
PctI GAATGC 1 cut(s) 771
PdmI GAANNNNTTC 1 cut(s) 438
PfoI TCCNGGA 2 cut(s) 429, 738
PleI GAGTC 3 cut(s) 761, 899, 1312
PmaCI CACGTG 1 cut(s) 830
PmlI CACGTG 1 cut(s) 830
PpsI GAGTC 3 cut(s) 761, 899, 1312
Ppu21I YACGTR 2 cut(s) 503, 830
PscI ACATGT 1 cut(s) 1282
PsiI TTATAA 2 cut(s) 336, 1337
Psp6I CCWGG 2 cut(s) 429, 738
PspCI CACGTG 1 cut(s) 830
PspFI CCCAGC 2 cut(s) 535, 787
PspGI CCWGG 2 cut(s) 429, 738
PspN4I GGNNCC 1 cut(s) 84
PstI CTGCAG 1 cut(s) 166
PstNI CAGNNNCTG 1 cut(s) 320
PvuII CAGCTG 2 cut(s) 263, 535
RsaI GTAC 2 cut(s) 84, 237
RsaNI GTAC 2 cut(s) 83, 236
RseI CAYNNNNRTG 3 cut(s) 420, 606, 966
SapI GCTCTTC 1 cut(s) 191
SaqAI TTAA 1 cut(s) 1017
Sau3AI GATC 4 cut(s) 796, 853, 1234, 1348
SchI GAGTC 3 cut(s) 762, 899, 1313
ScrFI CCNGG 2 cut(s) 431, 740
SduI GDGCHC 1 cut(s) 35
SfaNI GCATC 4 cut(s) 34, 79, 121, 133
SfcI CTRYAG 2 cut(s) 135, 162
SfuI TTCGAA 1 cut(s) 811
SmiMI CAYNNNNRTG 3 cut(s) 420, 606, 966
Sse9I AATT 9 cut(s) 4, 287, 540, 668, 865, 1159, 1226, 1259, 1308
SsiI CCGC 4 cut(s) 185, 311, 317, 592
SspI AATATT 3 cut(s) 553, 718, 893
SspMI CTAG 4 cut(s) 56, 375, 996, 1077
StyD4I CCNGG 2 cut(s) 429, 738
StyI CCWWGG 1 cut(s) 709
TaaI ACNGT 1 cut(s) 341
TaiI ACGT 3 cut(s) 505, 574, 832
TaqI TCGA 2 cut(s) 811, 1200
TaqII GACCGA 1 cut(s) 413
TasI AATT 9 cut(s) 4, 287, 540, 668, 865, 1159, 1226, 1259, 1308
TauI GCSGC 3 cut(s) 188, 314, 320
Tru1I TTAA 1 cut(s) 1017
Tru9I TTAA 1 cut(s) 1017
TscAI CASTG 1 cut(s) 1358
TseFI GTSAC 3 cut(s) 369, 473, 654
TseI GCWGC 7 cut(s) 161, 164, 182, 314, 532, 1142, 1313
Tsp45I GTSAC 3 cut(s) 369, 473, 654
TspRI CASTG 1 cut(s) 1358
XapI RAATTY 4 cut(s) 4, 540, 668, 865
XceI RCATGY 4 cut(s) 55, 499, 1149, 1286
XmnI GAANNNNTTC 1 cut(s) 438
XspI CTAG 4 cut(s) 56, 375, 996, 1077
Zsp2I ATGCAT 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.