Rh1CG005100
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0006438
Physical Location & Seq
Forward (+)
845526 .. 846083
558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG005100.1

Sequence Viewer

Length: 558 bp
ATGGATGATTTCGACTTAGAACTGGATGAGATGGAATTAACTGCAGCAGCGGCTGGGTACCATTATTATAGCAGTATTACCAAGCAACCTTGTCATAGTTTGTCACCAAGTAAAGGATGTGGATTTATGACAGAGGTGCTAAATGGTCATGATGTTGTGTTCCAGGAAATGTTTCGGATGGATAAAAATGTATTTCACAAATTGTGTGACATTCTTCGGCGCAGAGGCATGTTGCGTGATACTGCTGGTGTTATGATAGAGGAGCAGCTTGGAATATTTTTGAATATTATTGGCCATAATGAGCGGAATAGAGTAATACAAGAGCGGTTTCATCACTCAGGTGAAACCATCAGCCGTCATTTCAACAATGTGTTGAAGGCTGTCAAGTCGCTTTCGCGTGAATTTTTACAAACTCCAAGCCCCAGCACTCCTCCAGAAATCCTTGGAAATAACAGATTTTATCCTTATTTCCAGGTTTGTATTTTGTTGCGTAATTCTATCAATTCACTTGTCGCTTGGCTATTTTTTTTTTTCCATTCACTTTTACTTGCTACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

185

Amino Acids

21.39

Weight (kDa)

6.49

Isoelectric Point (pI)

58.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 37 - 129 3e-37 Domain of unknown function (DUF8040)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 57
AccB1I GGYRCC 1 cut(s) 57
AccBSI CCGCTC 2 cut(s) 304, 325
AccII CGCG 1 cut(s) 397
AciI CCGC 3 cut(s) 50, 304, 325
AcoI YGGCCR 1 cut(s) 292
AcsI RAATTY 1 cut(s) 401
AfaI GTAC 1 cut(s) 59
AfiI CCNNNNNNNGG 1 cut(s) 113
AgsI TTSAA 3 cut(s) 283, 364, 376
AjnI CCWGG 2 cut(s) 162, 471
AleI CACNNNNGTG 1 cut(s) 339
AluBI AGCT 1 cut(s) 268
AluI AGCT 1 cut(s) 268
AlwNI CAGNNNCTG 1 cut(s) 53
AoxI GGCC 1 cut(s) 292
ApeKI GCWGC 3 cut(s) 44, 47, 265
ApoI RAATTY 1 cut(s) 401
Asp700I GAANNNNTTC 1 cut(s) 171
Asp718I GGTACC 1 cut(s) 57
AspLEI GCGC 1 cut(s) 222
AsuHPI GGTGA 2 cut(s) 96, 353
BalI TGGCCA 1 cut(s) 294
BanI GGYRCC 1 cut(s) 57
BbvI GCAGC 3 cut(s) 56, 59, 277
BccI CCATC 3 cut(s) 25, 172, 356
BceAI ACGGC 1 cut(s) 339
BciT130I CCWGG 2 cut(s) 164, 473
BfmI CTRYAG 1 cut(s) 42
BisI GCNGC 4 cut(s) 45, 48, 51, 266
BlsI GCNGC 4 cut(s) 46, 49, 52, 267
Bme1390I CCNGG 2 cut(s) 164, 473
BmiI GGNNCC 1 cut(s) 59
BmrFI CCNGG 2 cut(s) 164, 473
BpmI CTGGAG 1 cut(s) 417
BsaJI CCNNGG 1 cut(s) 442
Bsc4I CCNNNNNNNGG 1 cut(s) 113
Bse1I ACTGG 1 cut(s) 27
BseBI CCWGG 2 cut(s) 164, 473
BseDI CCNNGG 1 cut(s) 442
BseGI GGATG 4 cut(s) 10, 31, 122, 183
BseLI CCNNNNNNNGG 1 cut(s) 113
BseMII CTCAG 1 cut(s) 351
BseNI ACTGG 1 cut(s) 27
BseRI GAGGAG 2 cut(s) 275, 420
BseXI GCAGC 3 cut(s) 56, 59, 277
BseYI CCCAGC 2 cut(s) 53, 422
Bsh1236I CGCG 1 cut(s) 397
BshFI GGCC 1 cut(s) 294
BshNI GGYRCC 1 cut(s) 57
BslI CCNNNNNNNGG 1 cut(s) 113
BsnI GGCC 1 cut(s) 294
BspACI CCGC 3 cut(s) 50, 304, 325
BspANI GGCC 1 cut(s) 294
BspCNI CTCAG 1 cut(s) 350
BspFNI CGCG 1 cut(s) 397
BspHI TCATGA 1 cut(s) 148
BspLI GGNNCC 1 cut(s) 59
BspMAI CTGCAG 1 cut(s) 46
BspT107I GGYRCC 1 cut(s) 57
BsrBI CCGCTC 2 cut(s) 304, 325
BsrI ACTGG 1 cut(s) 27
BssECI CCNNGG 1 cut(s) 442
BssT1I CCWWGG 1 cut(s) 442
Bst2UI CCWGG 2 cut(s) 164, 473
BstDEI CTNAG 2 cut(s) 16, 337
BstF5I GGATG 4 cut(s) 10, 31, 122, 183
BstFNI CGCG 1 cut(s) 397
BstHHI GCGC 1 cut(s) 222
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstNI CCWGG 2 cut(s) 164, 473
BstNSI RCATGY 1 cut(s) 232
BstSCI CCNGG 2 cut(s) 162, 471
BstSFI CTRYAG 1 cut(s) 42
BstUI CGCG 1 cut(s) 397
BstV1I GCAGC 3 cut(s) 56, 59, 277
BsuRI GGCC 1 cut(s) 294
BtsCI GGATG 4 cut(s) 10, 31, 122, 183
CaiI CAGNNNCTG 1 cut(s) 53
CciI TCATGA 1 cut(s) 148
CfoI GCGC 1 cut(s) 222
Csp6I GTAC 1 cut(s) 58
CviAII CATG 2 cut(s) 149, 229
CviJI RGCY 7 cut(s) 53, 268, 294, 354, 380, 420, 520
CviKI_1 RGCY 7 cut(s) 53, 268, 294, 354, 380, 420, 520
CviQI GTAC 1 cut(s) 58
DdeI CTNAG 2 cut(s) 16, 337
EaeI YGGCCR 1 cut(s) 292
Eco130I CCWWGG 1 cut(s) 442
EcoRII CCWGG 2 cut(s) 162, 471
EcoT14I CCWWGG 1 cut(s) 442
ErhI CCWWGG 1 cut(s) 442
FaeI CATG 2 cut(s) 152, 232
FaiI YATR 7 cut(s) 69, 96, 128, 150, 230, 254, 297
FatI CATG 2 cut(s) 148, 228
Fnu4HI GCNGC 4 cut(s) 45, 48, 51, 266
FokI GGATG 4 cut(s) 17, 38, 129, 190
Fsp4HI GCNGC 4 cut(s) 45, 48, 51, 266
GlaI GCGC 1 cut(s) 221
GluI GCNGC 4 cut(s) 45, 48, 51, 266
GsaI CCCAGC 2 cut(s) 57, 426
GsuI CTGGAG 1 cut(s) 417
HaeIII GGCC 1 cut(s) 294
HhaI GCGC 1 cut(s) 222
Hin1II CATG 2 cut(s) 152, 232
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HphI GGTGA 2 cut(s) 96, 353
Hpy188I TCNGA 1 cut(s) 177
Hpy188III TCNNGA 2 cut(s) 149, 434
HpyAV CCTTC 1 cut(s) 370
HpyCH4V TGCA 1 cut(s) 44
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpyF3I CTNAG 2 cut(s) 16, 337
Hsp92II CATG 2 cut(s) 152, 232
HspAI GCGC 1 cut(s) 220
KpnI GGTACC 1 cut(s) 61
LmnI GCTCC 1 cut(s) 262
Lsp1109I GCAGC 3 cut(s) 56, 59, 277
MaeIII GTNAC 2 cut(s) 102, 206
MbiI CCGCTC 2 cut(s) 304, 325
MboII GAAGA 1 cut(s) 206
MlsI TGGCCA 1 cut(s) 294
MluCI AATT 5 cut(s) 35, 200, 401, 493, 502
MluNI TGGCCA 1 cut(s) 294
MnlI CCTC 4 cut(s) 127, 218, 253, 441
Mox20I TGGCCA 1 cut(s) 294
MroXI GAANNNNTTC 1 cut(s) 171
MscI TGGCCA 1 cut(s) 294
MseI TTAA 1 cut(s) 38
MslI CAYNNNNRTG 1 cut(s) 339
Msp20I TGGCCA 1 cut(s) 294
MspA1I CMGCKG 1 cut(s) 50
MspR9I CCNGG 2 cut(s) 164, 473
MvaI CCWGG 2 cut(s) 164, 473
MvnI CGCG 1 cut(s) 397
MwoI GCNNNNNNNGC 1 cut(s) 50
NlaIII CATG 2 cut(s) 152, 232
NlaIV GGNNCC 1 cut(s) 59
NmuCI GTSAC 2 cut(s) 102, 206
NspI RCATGY 1 cut(s) 232
OliI CACNNNNGTG 1 cut(s) 339
PagI TCATGA 1 cut(s) 148
PdmI GAANNNNTTC 1 cut(s) 171
PfoI TCCNGGA 1 cut(s) 162
PkrI GCNGC 4 cut(s) 46, 49, 52, 267
Psp6I CCWGG 2 cut(s) 162, 471
PspFI CCCAGC 2 cut(s) 53, 422
PspGI CCWGG 2 cut(s) 162, 471
PspN4I GGNNCC 1 cut(s) 59
PstI CTGCAG 1 cut(s) 46
PstNI CAGNNNCTG 1 cut(s) 53
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
RseI CAYNNNNRTG 1 cut(s) 339
SaqAI TTAA 1 cut(s) 38
SatI GCNGC 4 cut(s) 45, 48, 51, 266
ScrFI CCNGG 2 cut(s) 164, 473
SetI ASST 6 cut(s) 91, 138, 270, 343, 477, 557
SfcI CTRYAG 1 cut(s) 42
SmiMI CAYNNNNRTG 1 cut(s) 339
Sse9I AATT 5 cut(s) 35, 200, 401, 493, 502
SsiI CCGC 3 cut(s) 50, 304, 325
SspI AATATT 2 cut(s) 276, 286
StyD4I CCNGG 2 cut(s) 162, 471
StyI CCWWGG 1 cut(s) 442
TaqI TCGA 1 cut(s) 12
TasI AATT 5 cut(s) 35, 200, 401, 493, 502
TauI GCSGC 1 cut(s) 53
Tru1I TTAA 1 cut(s) 38
Tru9I TTAA 1 cut(s) 38
TseFI GTSAC 2 cut(s) 102, 206
TseI GCWGC 3 cut(s) 44, 47, 265
Tsp45I GTSAC 2 cut(s) 102, 206
TspDTI ATGAA 1 cut(s) 320
XapI RAATTY 1 cut(s) 401
XceI RCATGY 1 cut(s) 232
XmnI GAANNNNTTC 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.