pycom06g20420
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0006438
Physical Location & Seq
Forward (+)
23462800 .. 23463216
417 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g20420.2

Sequence Viewer

Length: 342 bp
ATGGATAAGCACGTCTTCTACAAGTTGTGTGATATTTTGCAAGGAAAAGGCCTTCTGCGTCACACAAATCGAATCAAGATTGAAGAGCAACTGGCCATGTTCTTGTTCATAATTGGTCATAATCTACGGACTCGAGCTGTTCAAGAGTTATTCCGTTATTCAGGAGAAACCATCAGTCGTCATTTCAACAATGTCTTGAACGCAGTAATGGCAATTTCGTTAGGTTTCTTTCAGCCTCCAGGATCGATCATGATGTTCCTCCCAAAATTTCAGAAGATCCTAGATTCTATCCATATTTTAAGGATTGTGTGGGAGCAGTTGATGGCATACACCTCCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

114

Amino Acids

13.27

Weight (kDa)

9.73

Isoelectric Point (pI)

51.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 250, 271
AcoI YGGCCR 1 cut(s) 93
AcsI RAATTY 1 cut(s) 266
AgsI TTSAA 4 cut(s) 83, 143, 187, 199
AjiI CACGTC 1 cut(s) 13
AjnI CCWGG 1 cut(s) 238
AluBI AGCT 1 cut(s) 137
AluI AGCT 1 cut(s) 137
AlwI GGATC 2 cut(s) 250, 271
Ama87I CYCGRG 1 cut(s) 132
AoxI GGCC 2 cut(s) 49, 93
ApoI RAATTY 1 cut(s) 266
AvaI CYCGRG 1 cut(s) 132
BalI TGGCCA 1 cut(s) 95
BbsI GAAGAC 1 cut(s) 7
BccI CCATC 2 cut(s) 179, 316
BciT130I CCWGG 1 cut(s) 240
BfaI CTAG 1 cut(s) 281
Bme1390I CCNGG 1 cut(s) 240
BmeT110I CYCGRG 1 cut(s) 132
BmgBI CACGTC 1 cut(s) 13
BmrFI CCNGG 1 cut(s) 240
BmrI ACTGGG 1 cut(s) 331
BmuI ACTGGG 1 cut(s) 331
BpiI GAAGAC 1 cut(s) 7
BpmI CTGGAG 1 cut(s) 222
Bsa29I ATCGAT 1 cut(s) 245
Bse1I ACTGG 2 cut(s) 96, 337
BseBI CCWGG 1 cut(s) 240
BseCI ATCGAT 1 cut(s) 245
BseNI ACTGG 2 cut(s) 96, 337
BshFI GGCC 2 cut(s) 51, 95
BshVI ATCGAT 1 cut(s) 245
BsiHKCI CYCGRG 1 cut(s) 132
BsnI GGCC 2 cut(s) 51, 95
BsoBI CYCGRG 1 cut(s) 132
Bsp143I GATC 3 cut(s) 242, 246, 276
BspANI GGCC 2 cut(s) 51, 95
BspDI ATCGAT 1 cut(s) 245
BspHI TCATGA 1 cut(s) 249
BspPI GGATC 2 cut(s) 250, 271
BspQI GCTCTTC 1 cut(s) 78
BsrI ACTGG 2 cut(s) 96, 337
BssMI GATC 3 cut(s) 242, 246, 276
Bst2UI CCWGG 1 cut(s) 240
Bst6I CTCTTC 1 cut(s) 78
BstKTI GATC 3 cut(s) 245, 249, 279
BstMBI GATC 3 cut(s) 242, 246, 276
BstMWI GCNNNNNNNGC 1 cut(s) 209
BstNI CCWGG 1 cut(s) 240
BstSCI CCNGG 1 cut(s) 238
BstV2I GAAGAC 1 cut(s) 7
BstX2I RGATCY 1 cut(s) 276
BstYI RGATCY 1 cut(s) 276
Bsu15I ATCGAT 1 cut(s) 245
BsuRI GGCC 2 cut(s) 51, 95
BsuTUI ATCGAT 1 cut(s) 245
BtrI CACGTC 1 cut(s) 13
CciI TCATGA 1 cut(s) 249
ClaI ATCGAT 1 cut(s) 245
CseI GACGC 1 cut(s) 47
CviAII CATG 2 cut(s) 97, 250
CviJI RGCY 4 cut(s) 51, 95, 137, 235
CviKI_1 RGCY 4 cut(s) 51, 95, 137, 235
DpnI GATC 3 cut(s) 244, 248, 278
DpnII GATC 3 cut(s) 242, 246, 276
EaeI YGGCCR 1 cut(s) 93
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
Eco147I AGGCCT 1 cut(s) 51
Eco88I CYCGRG 1 cut(s) 132
EcoRII CCWGG 1 cut(s) 238
FaeI CATG 2 cut(s) 100, 253
FaiI YATR 6 cut(s) 98, 110, 120, 251, 294, 328
FalI AAGNNNNNCTT 1 cut(s) 31
FatI CATG 2 cut(s) 96, 249
FspBI CTAG 1 cut(s) 281
GsuI CTGGAG 1 cut(s) 222
HaeIII GGCC 2 cut(s) 51, 95
HgaI GACGC 1 cut(s) 47
Hin1II CATG 2 cut(s) 100, 253
HinfI GANTC 3 cut(s) 72, 130, 284
Hpy188I TCNGA 1 cut(s) 273
Hpy188III TCNNGA 5 cut(s) 76, 143, 162, 196, 250
HpyAV CCTTC 1 cut(s) 62
HpyCH4IV ACGT 1 cut(s) 12
HpyCH4V TGCA 1 cut(s) 40
HpyF10VI GCNNNNNNNGC 1 cut(s) 209
HpySE526I ACGT 1 cut(s) 12
Hsp92II CATG 2 cut(s) 100, 253
Kzo9I GATC 3 cut(s) 242, 246, 276
LguI GCTCTTC 1 cut(s) 78
LmnI GCTCC 1 cut(s) 313
LpnPI CCDG 4 cut(s) 77, 147, 225, 252
MaeI CTAG 1 cut(s) 281
MaeII ACGT 1 cut(s) 12
MaeIII GTNAC 1 cut(s) 59
MalI GATC 3 cut(s) 244, 248, 278
MboI GATC 3 cut(s) 242, 246, 276
MboII GAAGA 3 cut(s) 7, 95, 286
MflI RGATCY 1 cut(s) 276
MlsI TGGCCA 1 cut(s) 95
MluCI AATT 3 cut(s) 111, 213, 266
MluNI TGGCCA 1 cut(s) 95
MlyI GAGTC 1 cut(s) 124
MnlI CCTC 2 cut(s) 246, 269
Mox20I TGGCCA 1 cut(s) 95
MscI TGGCCA 1 cut(s) 95
MseI TTAA 1 cut(s) 299
Msp20I TGGCCA 1 cut(s) 95
MspR9I CCNGG 1 cut(s) 240
MvaI CCWGG 1 cut(s) 240
MwoI GCNNNNNNNGC 1 cut(s) 209
NdeII GATC 3 cut(s) 242, 246, 276
NlaIII CATG 2 cut(s) 100, 253
NmuCI GTSAC 1 cut(s) 59
PaeR7I CTCGAG 1 cut(s) 132
PagI TCATGA 1 cut(s) 249
PceI AGGCCT 1 cut(s) 51
PciSI GCTCTTC 1 cut(s) 78
PfeI GAWTC 2 cut(s) 72, 284
PfoI TCCNGGA 1 cut(s) 238
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PspXI VCTCGAGB 1 cut(s) 132
PsuI RGATCY 1 cut(s) 276
SapI GCTCTTC 1 cut(s) 78
SaqAI TTAA 1 cut(s) 299
Sau3AI GATC 3 cut(s) 242, 246, 276
SchI GAGTC 1 cut(s) 124
ScrFI CCNGG 1 cut(s) 240
SetI ASST 4 cut(s) 15, 139, 226, 335
Sfr274I CTCGAG 1 cut(s) 132
SlaI CTCGAG 1 cut(s) 132
SmlI CTYRAG 1 cut(s) 132
SmoI CTYRAG 1 cut(s) 132
Sse9I AATT 3 cut(s) 111, 213, 266
SseBI AGGCCT 1 cut(s) 51
SspMI CTAG 1 cut(s) 281
StuI AGGCCT 1 cut(s) 51
StyD4I CCNGG 1 cut(s) 238
TaiI ACGT 1 cut(s) 15
TaqI TCGA 3 cut(s) 70, 133, 245
TasI AATT 3 cut(s) 111, 213, 266
TfiI GAWTC 2 cut(s) 72, 284
Tru1I TTAA 1 cut(s) 299
Tru9I TTAA 1 cut(s) 299
TseFI GTSAC 1 cut(s) 59
Tsp45I GTSAC 1 cut(s) 59
TspDTI ATGAA 1 cut(s) 97
TspGWI ACGGA 2 cut(s) 142, 143
XapI RAATTY 1 cut(s) 266
XhoI CTCGAG 1 cut(s) 132
XspI CTAG 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.