Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr14

Orthogroup ID

OG0006438

Physical Location & Seq

Genomic Coordinates Forward (+)

21039544 .. 21043060

Gene Structure 3517 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom14g19810.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1527 bp

ATGAATAACTTAACATCATTTCGAGTCAAAGTAACAGTAGCAAAAGAAATTGCCAGCCCACCCTCCTCCCCCAGTCTGAAGTTTGCAGAAGACTGCAAATTACAAATTTATCGAGAAAATAAAAAGGAGAGAGCGCGAGCGACGGCAAAGTGCGAAAGCAAACGCACAGAATCAACGGAGAATGTGACGTCACTCAGTGGCTCACCTCACTCACTCATAACTCCCTTAAATTCAAAAACCCAACGATTCTGTTCATCTCACACATTTCTCTCGTTCCGCCCCCAAGCCGCCGGCGACGACGTCGTTTGCCTCCTGAAGTTGCAGAAGCTCAGCTCTCCGCAGTTGGTAATGGAGATCTCTGAAGATGAAAAGGATACAGTCTATGAGAATTATATACCGAAAGAACTTAGTCATGCTTTATCATCTACTGGTGCAAAGTTTATAGATGAAGTCCTTAATGGTCAAAATGAACGCTGTTTAGAGAACTTTCGCATGGATAAGCATGTCTTCTACAAGTTGTGTGATATTTTGCAAGGAAAAGGCCTACTGCGCCACACAAATCGAATCAAGATTGAAGAGCAATTGGCCATGTTCTTGTTCATAATTGGTCATAATCTACGGACCCGAGCTGTTCAAGAGTTATTTCGTTATTCAGGAGAAACCATTAGTCGTCATTTCAACAATGTCTTAAACGCAGTAATGGCAATTTCGATAGGTTTCTTTCAGCCTCCAGGATCTGCTGTTCCTCCCGAAATTTCAGAAAATCCTAGATTTTATCCATATTTTAAGGATTGTGTGGGAGCAGTTGATGGCATACACTTCCCAGTGATGGTGGGGGTAGATGAGCAAGGACCTTTCCGCGACAAGAATGGCTTACTTTCACAAAATGTTCTGGCTGCTTGCTCATTTGATCTCAAGTTCCTTTATGTCTTGGCTGGTTGGGAAGGCTCTGCTTCAGATTTGCAAGTTTTGAATTCTGCTCTCACCAGACGAAACAAATTGCAGATGCCAGAAGGGAAATACTACCTCGTGGACAAGAAGTATCCAAACATGCCTGGCTTCATTGCTCCATATACTGGTATACCTTATCACTCAACGGAGTTTCCTAGTGGTTATCACCCTCAAGATGCAAAAGAGCTATTTAATCAGCGACACTCATTGCTACGAAATGCATCTGATCGCACTTTTGGGGCTTTAAAAGAACGTTTCCCTATATTGATGTCAGCCCCTCCGTACCCATTACAAACACAAGTCAAATTGGTTGTGGCAGCTTGTGCCCTACACAACTTCATCCGTGAGGAGAAACCAGATGACTGGATCTTTAAAATGTACGAAAAGGACACCATACTGCAAATGGAGGAATCAATTCCCCCGTTAGCAGAGGCGGAGCAACCAATCATTGCTTTTGAAACCTCTGCTTTGGACATTGCTTTTGAAACAGAAGAACTGGAATTCACTTCGCAGATGCGGGATTCGATTGCAAGTGAAATGTGGGATGACTATATCCATGATTTATCTCCCATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DDE_Tnp_4

Protein Analysis

509

Amino Acids

57.67

Weight (kDa)

5.88

Isoelectric Point (pI)

47.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF8040	PF26138	142 - 234	2.8e-38	Domain of unknown function (DUF8040)
Plant_tran	PF04827	265 - 435	6.9e-08	Plant transposon protein
DDE_Tnp_4	PF13359	269 - 429	3.4e-19	DDE superfamily endonuclease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006438)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G41980
fragaria_vesca	FvH4_4g05911 FvH4_4g05911 FvH4_4g05911 FvH4_4g05911 FvH4_4g05911 FvH4_4g05911 FvH4_4g05911
malus_domestica	MD00G1159500.v1.1 MD06G1227900.v1.1 MD14G1237100.v1.1
prunus_persica	Prupe.5G234400_v2.0.a1 Prupe.7G000100_v2.0.a1
pyrus_communis	pycom06g20420 pycom06g20430 pycom12g00010 pycom14g19810
rosa_chinensis	RchiOBHm_Chr1g0313091 RchiOBHm_Chr6g0248041
rosa_multiflora	Rmu_sc0001606.1_g000006 Rmu_sc0030940.1_g000001
rosa_roxburghii	Rroxscaffold_4G00332540
rosa_samantha	Rh1CG001900 Rh1CG005100 Rh6BG040400
rosa_wichuraiana	Rw0G008470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	191, 303
AccB7I	CCANNNNNTGG	1 cut(s)	1076
AccI	GTMKAC	1 cut(s)	1081
AccII	CGCG	2 cut(s)	136, 861
AciI	CCGC	6 cut(s)	277, 288, 338, 859, 1385, 1468
AclI	AACGTT	1 cut(s)	1204
AclWI	GGATC	2 cut(s)	742, 1325
AcoI	YGGCCR	1 cut(s)	585
AcsI	RAATTY	5 cut(s)	105, 229, 753, 973, 1451
AcuI	CTGAAG	4 cut(s)	98, 335, 381, 939
AcyI	GRCGYC	2 cut(s)	188, 300
AdeI	CACNNNGTG	1 cut(s)	197
AfaI	GTAC	2 cut(s)	1235, 1331
AfiI	CCNNNNNNNGG	2 cut(s)	829, 1076
AgsI	TTSAA	7 cut(s)	234, 575, 635, 679, 973, 1409, 1436
AjnI	CCWGG	2 cut(s)	730, 1054
AloI	GAACNNNNNNTCC	2 cut(s)	726, 758
AluBI	AGCT	5 cut(s)	328, 333, 629, 1138, 1271
AluI	AGCT	5 cut(s)	328, 333, 629, 1138, 1271
AlwI	GGATC	2 cut(s)	742, 1325
AlwNI	CAGNNNCTG	1 cut(s)	737
Ama87I	CYCGRG	1 cut(s)	624
AoxI	GGCC	2 cut(s)	541, 585
ApeKI	GCWGC	2 cut(s)	896, 1268
ApoI	RAATTY	5 cut(s)	105, 229, 753, 973, 1451
ArsI	GACNNNNNNTTYG	2 cut(s)	1415, 1447
Asp700I	GAANNNNTTC	1 cut(s)	1365
AspLEI	GCGC	2 cut(s)	136, 552
AspS9I	GGNCC	2 cut(s)	621, 851
AsuHPI	GGTGA	3 cut(s)	195, 976, 1109
AvaI	CYCGRG	1 cut(s)	624
AvaII	GGWCC	2 cut(s)	621, 851
BaeGI	GKGCMC	1 cut(s)	1279
BalI	TGGCCA	1 cut(s)	587
BauI	CACGAG	1 cut(s)	1028
BbsI	GAAGAC	2 cut(s)	96, 499
BbvI	GCAGC	2 cut(s)	883, 1280
BccI	CCATC	2 cut(s)	803, 823
BceAI	ACGGC	1 cut(s)	159
BciT130I	CCWGG	2 cut(s)	732, 1056
BciVI	GTATCC	2 cut(s)	367, 1053
BfaI	CTAG	2 cut(s)	768, 1107
BfuI	GTATCC	2 cut(s)	367, 1053
BglII	AGATCT	1 cut(s)	354
BisI	GCNGC	3 cut(s)	288, 897, 1269
BlpI	GCTNAGC	1 cut(s)	329
BlsI	GCNGC	3 cut(s)	289, 898, 1270
Bme1390I	CCNGG	2 cut(s)	732, 1056
Bme18I	GGWCC	2 cut(s)	621, 851
BmeT110I	CYCGRG	1 cut(s)	624
BmgT120I	GGNCC	2 cut(s)	621, 851
BmiI	GGNNCC	1 cut(s)	623
BmrFI	CCNGG	2 cut(s)	732, 1056
BmrI	ACTGGG	2 cut(s)	66, 818
BmsI	GCATC	4 cut(s)	996, 1117, 1181, 1455
BmuI	ACTGGG	2 cut(s)	66, 818
BpiI	GAAGAC	2 cut(s)	96, 499
BpmI	CTGGAG	1 cut(s)	714
Bpu1102I	GCTNAGC	1 cut(s)	329
BpuEI	CTTGAG	2 cut(s)	899, 1107
BsaHI	GRCGYC	2 cut(s)	188, 300
Bsc4I	CCNNNNNNNGG	2 cut(s)	829, 1076
Bse118I	RCCGGY	1 cut(s)	290
Bse1I	ACTGG	6 cut(s)	72, 433, 824, 1081, 1319, 1452
Bse3DI	GCAATG	4 cut(s)	1062, 1157, 1398, 1425
BseBI	CCWGG	2 cut(s)	732, 1056
BseGI	GGATG	2 cut(s)	1290, 1501
BseLI	CCNNNNNNNGG	2 cut(s)	829, 1076
BseMI	GCAATG	4 cut(s)	1062, 1157, 1398, 1425
BseMII	CTCAG	2 cut(s)	208, 343
BseNI	ACTGG	6 cut(s)	72, 433, 824, 1081, 1319, 1452
BseRI	GAGGAG	2 cut(s)	55, 1313
BseSI	GKGCMC	1 cut(s)	1279
BseXI	GCAGC	2 cut(s)	883, 1280
Bsh1236I	CGCG	2 cut(s)	136, 861
BshFI	GGCC	2 cut(s)	543, 587
BsiHKCI	CYCGRG	1 cut(s)	624
BsiSI	CCGG	1 cut(s)	291
BslI	CCNNNNNNNGG	2 cut(s)	829, 1076
BsnI	GGCC	2 cut(s)	543, 587
BsoBI	CYCGRG	1 cut(s)	624
Bsp1286I	GDGCHC	1 cut(s)	1279
Bsp143I	GATC	5 cut(s)	354, 734, 910, 1177, 1317
Bsp1720I	GCTNAGC	1 cut(s)	329
BspACI	CCGC	6 cut(s)	277, 288, 338, 859, 1385, 1468
BspANI	GGCC	2 cut(s)	543, 587
BspCNI	CTCAG	2 cut(s)	207, 342
BspFNI	CGCG	2 cut(s)	136, 861
BspLI	GGNNCC	1 cut(s)	623
BspPI	GGATC	2 cut(s)	742, 1325
BspQI	GCTCTTC	1 cut(s)	570
BsrDI	GCAATG	4 cut(s)	1062, 1157, 1398, 1425
BsrFI	RCCGGY	1 cut(s)	290
BsrI	ACTGG	6 cut(s)	72, 433, 824, 1081, 1319, 1452
BssAI	RCCGGY	1 cut(s)	290
BssMI	GATC	5 cut(s)	354, 734, 910, 1177, 1317
BssNAI	GTATAC	1 cut(s)	1082
BssNI	GRCGYC	2 cut(s)	188, 300
BssSI	CACGAG	1 cut(s)	1028
Bst1107I	GTATAC	1 cut(s)	1082
Bst2BI	CACGAG	1 cut(s)	1028
Bst2UI	CCWGG	2 cut(s)	732, 1056
Bst4CI	ACNGT	2 cut(s)	37, 379
Bst6I	CTCTTC	1 cut(s)	570
BstACI	GRCGYC	2 cut(s)	188, 300
BstAPI	GCANNNNNTGC	1 cut(s)	1274
BstC8I	GCNNGC	4 cut(s)	55, 138, 292, 901
BstDEI	CTNAG	3 cut(s)	194, 329, 407
BstF5I	GGATG	2 cut(s)	1290, 1501
BstFNI	CGCG	2 cut(s)	136, 861
BstHHI	GCGC	2 cut(s)	136, 552
BstKTI	GATC	5 cut(s)	357, 737, 913, 1180, 1320
BstMBI	GATC	5 cut(s)	354, 734, 910, 1177, 1317
BstMWI	GCNNNNNNNGC	3 cut(s)	549, 701, 1274
BstNI	CCWGG	2 cut(s)	732, 1056
BstNSI	RCATGY	2 cut(s)	506, 1054
BstSCI	CCNGG	2 cut(s)	730, 1054
BstSLI	GKGCMC	1 cut(s)	1279
BstUI	CGCG	2 cut(s)	136, 861
BstV1I	GCAGC	2 cut(s)	883, 1280
BstV2I	GAAGAC	2 cut(s)	96, 499
BstX2I	RGATCY	3 cut(s)	354, 734, 1317
BstXI	CCANNNNNNTGG	1 cut(s)	1314
BstYI	RGATCY	3 cut(s)	354, 734, 1317
BstZ17I	GTATAC	1 cut(s)	1082
BsuI	GTATCC	2 cut(s)	367, 1053
BsuRI	GGCC	2 cut(s)	543, 587
BtsCI	GGATG	2 cut(s)	1290, 1501
BtsIMutI	CAGTG	2 cut(s)	202, 831
Cac8I	GCNNGC	4 cut(s)	55, 138, 292, 901
CaiI	CAGNNNCTG	1 cut(s)	737
CfoI	GCGC	2 cut(s)	136, 552
Cfr10I	RCCGGY	1 cut(s)	290
Cfr13I	GGNCC	2 cut(s)	621, 851
Csp6I	GTAC	2 cut(s)	1234, 1330
CviAII	CATG	7 cut(s)	413, 493, 503, 589, 1051, 1508, 1522
CviQI	GTAC	2 cut(s)	1234, 1330
DdeI	CTNAG	3 cut(s)	194, 329, 407
DpnI	GATC	5 cut(s)	356, 736, 912, 1179, 1319
DpnII	GATC	5 cut(s)	354, 734, 910, 1177, 1317
DraI	TTTAAA	2 cut(s)	1197, 1324
DraIII	CACNNNGTG	1 cut(s)	197
EaeI	YGGCCR	1 cut(s)	585
Eam1104I	CTCTTC	1 cut(s)	570
EarI	CTCTTC	1 cut(s)	570
EciI	GGCGGA	2 cut(s)	266, 1400
Eco147I	AGGCCT	1 cut(s)	543
Eco47I	GGWCC	2 cut(s)	621, 851
Eco57I	CTGAAG	4 cut(s)	98, 335, 381, 939
Eco88I	CYCGRG	1 cut(s)	624
EcoO109I	RGGNCCY	1 cut(s)	851
EcoRI	GAATTC	2 cut(s)	973, 1451
EcoRII	CCWGG	2 cut(s)	730, 1054
EcoT22I	ATGCAT	1 cut(s)	1174
FaeI	CATG	7 cut(s)	416, 496, 506, 592, 1054, 1511, 1525
FalI	AAGNNNNNCTT	4 cut(s)	491, 523, 857, 889
FatI	CATG	7 cut(s)	412, 492, 502, 588, 1050, 1507, 1521
FauI	CCCGC	1 cut(s)	1461
FblI	GTMKAC	1 cut(s)	1081
Fnu4HI	GCNGC	3 cut(s)	288, 897, 1269
FokI	GGATG	2 cut(s)	1277, 1508
Fsp4HI	GCNGC	3 cut(s)	288, 897, 1269
FspBI	CTAG	2 cut(s)	768, 1107
GlaI	GCGC	2 cut(s)	135, 551
GluI	GCNGC	3 cut(s)	288, 897, 1269
GsuI	CTGGAG	1 cut(s)	714
HaeIII	GGCC	2 cut(s)	543, 587
HapII	CCGG	1 cut(s)	291
HhaI	GCGC	2 cut(s)	136, 552
Hin1I	GRCGYC	2 cut(s)	188, 300
Hin1II	CATG	7 cut(s)	416, 496, 506, 592, 1054, 1511, 1525
Hin6I	GCGC	2 cut(s)	134, 550
HinP1I	GCGC	2 cut(s)	134, 550
HinfI	GANTC	6 cut(s)	24, 170, 246, 564, 1361, 1472
HpaII	CCGG	1 cut(s)	291
HphI	GGTGA	3 cut(s)	195, 976, 1109
Hpy166II	GTNNAC	2 cut(s)	1033, 1082
Hpy188I	TCNGA	5 cut(s)	78, 361, 760, 958, 1177
Hpy188III	TCNNGA	7 cut(s)	113, 313, 568, 635, 654, 749, 1124
Hpy8I	GTNNAC	2 cut(s)	1033, 1082
Hpy99I	CGWCG	4 cut(s)	145, 299, 302, 305
HpyAV	CCTTC	2 cut(s)	938, 1007
HpyCH4III	ACNGT	2 cut(s)	37, 379
HpyCH4IV	ACGT	3 cut(s)	188, 300, 1204
HpyF10VI	GCNNNNNNNGC	3 cut(s)	549, 701, 1274
HpyF3I	CTNAG	3 cut(s)	194, 329, 407
HpySE526I	ACGT	3 cut(s)	188, 300, 1204
Hsp92I	GRCGYC	2 cut(s)	188, 300
Hsp92II	CATG	7 cut(s)	416, 496, 506, 592, 1054, 1511, 1525
HspAI	GCGC	2 cut(s)	134, 550
KroI	GCCGGC	1 cut(s)	290
KroNI	GCCGGC	1 cut(s)	292
Kzo9I	GATC	5 cut(s)	354, 734, 910, 1177, 1317
LguI	GCTCTTC	1 cut(s)	570
LmnI	GCTCC	3 cut(s)	800, 1072, 1387
Lsp1109I	GCAGC	2 cut(s)	883, 1280
LweI	GCATC	4 cut(s)	996, 1117, 1181, 1455
MaeI	CTAG	2 cut(s)	768, 1107
MaeII	ACGT	3 cut(s)	188, 300, 1204
MaeIII	GTNAC	3 cut(s)	31, 184, 189
MalI	GATC	5 cut(s)	356, 736, 912, 1179, 1319
MboI	GATC	5 cut(s)	354, 734, 910, 1177, 1317
MboII	GAAGA	5 cut(s)	101, 374, 499, 587, 1454
MfeI	CAATTG	1 cut(s)	581
MflI	RGATCY	3 cut(s)	354, 734, 1317
MhlI	GDGCHC	1 cut(s)	1279
MlsI	TGGCCA	1 cut(s)	587
MluNI	TGGCCA	1 cut(s)	587
MlyI	GAGTC	1 cut(s)	33
Mox20I	TGGCCA	1 cut(s)	587
Mph1103I	ATGCAT	1 cut(s)	1174
MreI	CGCCGGCG	1 cut(s)	290
MroNI	GCCGGC	1 cut(s)	290
MroXI	GAANNNNTTC	1 cut(s)	1365
MscI	TGGCCA	1 cut(s)	587
MseI	TTAA	8 cut(s)	11, 227, 456, 689, 786, 1143, 1196, 1323
Msp20I	TGGCCA	1 cut(s)	587
MspI	CCGG	1 cut(s)	291
MspR9I	CCNGG	2 cut(s)	732, 1056
MunI	CAATTG	1 cut(s)	581
MvaI	CCWGG	2 cut(s)	732, 1056
MvnI	CGCG	2 cut(s)	136, 861
MwoI	GCNNNNNNNGC	3 cut(s)	549, 701, 1274
NaeI	GCCGGC	1 cut(s)	292
NdeII	GATC	5 cut(s)	354, 734, 910, 1177, 1317
NgoMIV	GCCGGC	1 cut(s)	290
NlaIII	CATG	7 cut(s)	416, 496, 506, 592, 1054, 1511, 1525
NlaIV	GGNNCC	1 cut(s)	623
NmuCI	GTSAC	2 cut(s)	184, 189
NsiI	ATGCAT	1 cut(s)	1174
NspI	RCATGY	2 cut(s)	506, 1054
PceI	AGGCCT	1 cut(s)	543
PciSI	GCTCTTC	1 cut(s)	570
PdiI	GCCGGC	1 cut(s)	292
PdmI	GAANNNNTTC	1 cut(s)	1365
PfeI	GAWTC	5 cut(s)	170, 246, 564, 1361, 1472
PflFI	GACNNNGTC	1 cut(s)	299
PflMI	CCANNNNNTGG	1 cut(s)	1076
PfoI	TCCNGGA	1 cut(s)	730
PkrI	GCNGC	3 cut(s)	289, 898, 1270
PleI	GAGTC	1 cut(s)	32
PpsI	GAGTC	1 cut(s)	32
PpuMI	RGGWCCY	1 cut(s)	851
Psp1406I	AACGTT	1 cut(s)	1204
Psp5II	RGGWCCY	1 cut(s)	851
Psp6I	CCWGG	2 cut(s)	730, 1054
PspGI	CCWGG	2 cut(s)	730, 1054
PspN4I	GGNNCC	1 cut(s)	623
PspPI	GGNCC	2 cut(s)	621, 851
PspPPI	RGGWCCY	1 cut(s)	851
PstNI	CAGNNNCTG	1 cut(s)	737
PsuI	RGATCY	3 cut(s)	354, 734, 1317
PsyI	GACNNNGTC	1 cut(s)	299
RsaI	GTAC	2 cut(s)	1235, 1331
RsaNI	GTAC	2 cut(s)	1234, 1330
SapI	GCTCTTC	1 cut(s)	570
SaqAI	TTAA	8 cut(s)	11, 227, 456, 689, 786, 1143, 1196, 1323
SatI	GCNGC	3 cut(s)	288, 897, 1269
Sau3AI	GATC	5 cut(s)	354, 734, 910, 1177, 1317
Sau96I	GGNCC	2 cut(s)	621, 851
SchI	GAGTC	1 cut(s)	33
ScrFI	CCNGG	2 cut(s)	732, 1056
SduI	GDGCHC	1 cut(s)	1279
SfaNI	GCATC	4 cut(s)	996, 1117, 1181, 1455
SgrAI	CRCCGGYG	1 cut(s)	290
SinI	GGWCC	2 cut(s)	621, 851
SmlI	CTYRAG	2 cut(s)	914, 1122
SmoI	CTYRAG	2 cut(s)	914, 1122
SseBI	AGGCCT	1 cut(s)	543
SsiI	CCGC	6 cut(s)	277, 288, 338, 859, 1385, 1468
SspMI	CTAG	2 cut(s)	768, 1107
StuI	AGGCCT	1 cut(s)	543
StyD4I	CCNGG	2 cut(s)	730, 1054
TaaI	ACNGT	2 cut(s)	37, 379
TaiI	ACGT	3 cut(s)	191, 303, 1207
TaqI	TCGA	5 cut(s)	22, 112, 562, 710, 1475
TauI	GCSGC	1 cut(s)	290
TfiI	GAWTC	5 cut(s)	170, 246, 564, 1361, 1472
Tru1I	TTAA	8 cut(s)	11, 227, 456, 689, 786, 1143, 1196, 1323
Tru9I	TTAA	8 cut(s)	11, 227, 456, 689, 786, 1143, 1196, 1323
TscAI	CASTG	2 cut(s)	202, 831
TseFI	GTSAC	2 cut(s)	184, 189
TseI	GCWGC	2 cut(s)	896, 1268
Tsp45I	GTSAC	2 cut(s)	184, 189
TspDTI	ATGAA	8 cut(s)	17, 243, 381, 462, 483, 589, 1051, 1279
TspGWI	ACGGA	5 cut(s)	191, 634, 1112, 1221, 1283
TspRI	CASTG	2 cut(s)	202, 831
Tth111I	GACNNNGTC	1 cut(s)	299
Van91I	CCANNNNNTGG	1 cut(s)	1076
VpaK11BI	GGWCC	2 cut(s)	621, 851
XapI	RAATTY	5 cut(s)	105, 229, 753, 973, 1451
XceI	RCATGY	2 cut(s)	506, 1054
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1351
XmiI	GTMKAC	1 cut(s)	1081
XmnI	GAANNNNTTC	1 cut(s)	1365
XspI	CTAG	2 cut(s)	768, 1107
ZraI	GACGTC	2 cut(s)	189, 301
Zsp2I	ATGCAT	1 cut(s)	1174

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.